Hunh Nguyn Anh Khoa ng Th Tuyt n

Trng i hc Bch Khoa Tp. HCM, 12/2009

Ngun thu nhn protein

ng vt Thc vt Vi sinh vt

Protein a bo

Protein n bo

Protein

Cm t Protein n bo (Single Cell Protein SCP) c dng ln u tin bi gio s Carol Wilson nhm ch protein c ngun gc t vi sinh vt. (Ware, 1977) Protein n bo dng ch cc t bo vi sinh vt cht, c sy kh. (Israelidis, 2003)

V sao vic sn xut protein n bo tr thnh vn nng?

S thiu ht ngun protein a bo Nhng u im ca protein n bo m ra tim nng thay th ngun protein a bo

Nguyn nhn dn n thiu ht ngun protein a bo

Tc tng sn lng lng thc, thc phm khng theo kp tc tng dn s S phn phi ngun protein khng ng u gia cc ni trn th gii

Mi trng nhim lm mt dn ngun li

thc n t t nhin

Vi sinh vt l ngun cung cp thc n trong tng lai

Kh nng sinh sn v pht trin nhanh Hm lng protein cao (20-80%), thnh phn cn i v y D iu khin cht lng bng cch thay i

iu kin nui cy hoc tc ng vo h gene

Ch ng trong sn xut , t chim din tch

Nng sut thu nhn protein t vi sinh vt rt cao

Ngun thu nhn Sn lng (kg/ha/nm)
Spirulina platensis Chlorella pyrenoidosa Nm u nnh u phng 24,300 15,700 8,000 716 470

u H lan
La m Sa

395
300 100

Tht

60

c - nc tin phong sn xut SCP

Vic dng to lm thc n khng phi l chuyn mi. Trong CTTG I, Saccharomyces cerevisae v Candida utilis t b ln men ru, bia c thu nhn lm ngun cung cp protein. S pht trin ca ngnh cng nghip du m nhng nm 60 m ra kh nng sn sut SCP t ph phm du m Thp k 60 u thp k 80: Pht trin sn phm thc n gia sc 1985: Xut hin thc phm cho ngi t Fusarium graminearum

ng dn n tng lai
Nghin cu thu sinh khi r nhng c gi tr
dinh dng cao

Nghin cu thu thc n gia sc t CO2

hoc cellulose Nghin cu ln men t du m Nghin cu sn xut thc n cho ngi t nguyn liu r tin

Vi sinh vt trong sn xut protein n bo

Hai loi VSV tim nng l:

To n bo v To a bo
Nm men

Hm lng protein trong mt s loi vi sinh vt

Nhm % protein i din Spirulina sp. 70% 40-55% Saccharomyces 43-56% 30-75%

To

Nm men

Nm mc
Fusarium 40-45% 30-60%

Vi khun
-

Trung bnh

Sn xut sinh khi to thu nhn protein

Cc loi to tim nng: Vi khun lam Spirulina platensis Vi to Chlorella pyrenoidosa Vi to Aphanizomenon flos-aquae
Ngun c cht Cng ngh nui cy v thu nhn sinh khi Sn phm

Thnh phn dinh dng ca Spirulina platensis v Chlorella pyrenoidosa

% khi lng kh Protein Lipid Glucide Khong Spirulina platensis Chlorella pyrenoidosa

Amino acid (% KLK)

Arginine

Spirulina platensis

Chlorella pyrenoidosa

6.9 1.6 5.6 8.7 4.7 2.3 4.5 5.2

2.39 0.65 1.69 2 2.43 0.57 2.14 1.91

46-63 4-9 8-14 4-5

51-58 2 26 10-15

Histidine

Isoleucine
Leucine Lycine Methionine Phenylalanine

Threonine
Tryptophan Valine

1.5
6.5

0.41
2.67

CO2 t khng kh l ngun carbon gin tip trong sn xut sinh khi to

Nui trng to theo qui m cng nghip

Thu hoch sinh khi to

3 cng dng chnh ca SP to Spirulina

Tng cng sc khng ca c th
H lipid mu Tng hm lng IgA, IgM Gim t l mc bnh suy gim bch cu
(Hc vin Qun Y Trung Quc)

Ngun b sung dinh dng

Tng cng sc khe cho vn ng vin (Vin Thc vt V Hn, TQ) Linavina, Pirulamin, Spirulin: Sn phm cho tr suy dinh dng v ngi gi H cholesterol, chng bo ph (i hc Tokai, Nht)

Ngun thc phm

Sn xut sa chua, ph mai, u h Sn xut m n lin, bnh m

Nm men km u vit hn to trong sn xut thc phm Amino acid To Men bia L tng
D dng Thnh phn cc cht c hiu trong nm men khng
(FAO 1975) Cysteine Isoleucine Leucine

1.0 5.6 8.7

0.74 2.76 3.83

1.41 2.8 4

Lycine
Methionine Phenylalanine

4.7
2.3 4.5

3.53
1.05 2.29

1.9
1.68 3.67

hon ton ph hp
vi ngi

Threonine
Tryptophan Valine

5.2
1.5 6.5

3.26
0.82 3.23

2.60
0.94 0.36

B nm men ngun nguyn liu sn xut thc n gia sc

Sinh khi kh Sacchromyces cerevisae, Sacchromyces carlsbergensis t cng nghip ru, bia c b sung t 30 n 60% vo hn hp u nnh/bp lm thc n cho heo con Kt qu: nh hng ca hm lng b sung n s tng trng ca heo khng r rng Gim c chi ph/1kg tng trng ca heo
Slagle and Zimmerman (1979), Landell v cng s (1994), Ikurior (1995), Miyada v cng s (1997)

Nm men l i tng ch cht trong vic sn xut protein t du m

1925, Tauson pht hin ra kh nng phn gii
hydrocarbon ca vi sinh vt Cc vi sinh vt c kh nng phn gii hydrocarbon: Vi khun: Achrobacter, Corynebacterium, Mycobacterium, Pseudomonas, Nm men: Candida (C. lipolytica, C. intermedia, C. parasilopsis, C. utilis), Cytomyces, X khun,Nm si: Acremonium, Aspergilus, Actinomyces

Sn xut protein t du m, kh t vn cn nhiu hn ch

Parafin (20% trong du m) l loi nguyn liu d ln men nht Hiu sut chuyn ha c cht thnh protein thp Cn b sung ngun nit, khong

Sn phm hin nay ch c dng trong chn nui

Mt s sn phm b sung vo thc n gia sc

Sn phm Vi sinh vt

PRUTEEN TORUTEIN TOPRINA

Methyllophilus methylotropus Candida utilis Candida lipolytica

PEKILO

Paecilomyces variotii

Thc n nhn to cho ngi

Vn loi b tp cht t mi trng nui cy Vn c t t vi sinh vt Vn cn bng dinh dng Vn gi tr cm quan, th hiu ngi tiu dng

Nhim v ca nn khoa hc Vit Nam

Cc sn phm protein t VSV vn cn mt qung ng kh xa gp phn mnh m vo cuc chin y li nn i Protein Nhim v trc mt: Sn xut protein vi sinh vt dng dung dch hoc dng bt trng khng mi trn vo thc n Sn xut protein vi sinh vt dng th lm thc n gia sc, lm tng sn lng tht, sa, trngtrong khu phn n

Xin cm n s theo di!