Faculty of Applied Chemistry

Chit xut tinh du bc h v nh lng menthol

GVHD:

Nhm 1.1:
L Ngc Dim Bi Vn Hu L Th Nhi Nguyn Thanh Thy Tin

Nguyn Xun Th Dim Trinh

Ni dung
Tng quan
- Cy bc h
- Menthol

Faculty of Applied Chemistry

- Cy hng cy

Cc phng php chit xut tinh du nh tnh, nh lng menthol Thc nghim - Kt qu - Tho lun
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Gii thiu chung v cy bc h

Faculty of Applied Chemistry

Gii: Lp: B: H: Ging:

Plantae

Phn nhm: Magnoliophyta

Magnoliopsida Lamiales Lamiaceae Mentha

Loi:

Mentha piperita L,
Mt s b phn ca bc h.

Mentha arvensis L.
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Gii thiu chung v cy bc h Ngun gc v phn loi:

Bc h chu u (Mentha piperita L.):
Trng ch yu Anh. - Dng thn tm (bc h en, Black

Faculty of Applied Chemistry

Bc h Nht (Mentha arvensis L.): Trng nhiu Nht Bn, Trung Quc, n , Brazil - Dng thn tm - Dng thn xanh

mint ). Mentha piperita var. officinalis

forma rubescens Camus. - Dng thn xanh (bc h trng,

White mint). Mentha piperita var.

officinalis palles-cens Camus.

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Gii thiu chung v cy bc h Thnh phn ca tinh du bc h:

Faculty of Applied Chemistry

Trong bc h c: Menthol, Menthone, Menthyl Acetate,

Camphene, Limonene, Isomenthone, Pinene, Menthenone, Rosmarinic acid, d-Neomenthol, Ethyl-n-Amylketone, Piperitone, Piperitenone, Pulegone (Trung Dc Hc).

8/8/2012

Gii thiu chung v cy bc h Cng dng ca tinh du bc h:

Faculty of Applied Chemistry

Mt s cng dng ca tinh du bc h

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Menthol

Faculty of Applied Chemistry

Tn gi: (1, 2, 5) 5-methyl-2-(1-metyl etyl)-cyclohexanol, 3p-

Menthol; 1-Menthol; hexahydrotymol; peppermint camphor

L thnh phn chnh (60-90%) ca tinh du bc h

Menthol t do l thnh phn ch yu dng 1-Menthol vi nhm

CH3 v OH v tr trans so vi nhm C3H7

Cng thc cu to ca menthol

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Menthol
Tnh cht vt l:

Faculty of Applied Chemistry

L nhng tinh th khng mu, bng, hnh su cnh di, mng, mi v

c bit ging mi bc h

T trng: 0,890

Nhit nng chy: 42-44oC

Nhit si: 217oC quay cc D20 = -48o28 ha tan: rt d tan trong alcol, eter, cloroform, acid acetic, d tan
trong du m, parafin v hu nh khng tan trong nc
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Menthol
Tnh cht ha hc:
S th hin tnh cht ha hc ca menthol

Faculty of Applied Chemistry

8/8/2012

Menthol
C lp menthol t tinh du bc h:

Faculty of Applied Chemistry

Tinh du dng chit menthol phi c kh ht nc bng

Na2SO4 khan v lc trong.

Nguyn tc ca s ly trch menthol bao gm ba giai on:

1. Kt tinh menthol bng phng php lm lnh t tinh du ton phn. 2. Tch tinh th menthol ra khi s tinh du cn li bng s ly tm 3. Lm kh tinh th

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10

Hng cy

Faculty of Applied Chemistry

Tn khoa hc: Mentha arvensis L. var. javanica Hook

Tn thng: hng cy (hay Thy bc h)

H: Hoa mi (Lamiaceae)
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Hng cy
Cng dng

Faculty of Applied Chemistry

L hng cy c v cay thm, tnh

m, khng c, c tc dng gip n
ngon ming, kch thch cc tuyn m hi khin mt ngui, tiu phong nhit, thng t v, tr gip cho gan, phi.

Hng cy dng cha mt s bnh

nh cm st, nhc u, au bng, kh tiu,
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Hng cy
Tn cu phn - Phelandren - Pinen Sabinen - Pinen - Mircen 3- Octanol Limonen Linalol Trans--2,8-Mentadien Trans-Menton Cis-Menton C10H20O Menthol
8/8/2012

Faculty of Applied Chemistry

% cu phn Vt 0.06 0.12 0.93 0.26 0.49 20.70 0.21 0.50 0.73 0.20 0.51 1.37

Tn cu phn C7H12O C10H16O Carvol Carvon 3,7,7-Trimetilcaran C10H17 Acetat carvil Isocariophilen Cariophilen - Cariophilen C15H27 Oxid Cariophilen

% cu phn Vt 4.63 0.46 57.30 0.30 3.08 1.86 0.10 2.26 0.47 0.10 0.14
13

Hng cy

Faculty of Applied Chemistry

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Cc phng php sn xut tinh du

Faculty of Applied Chemistry

Nhng yu cu c ban khi san xut tinh du:

Tinh du thu c c mi v t nhin ban u

Qui trnh ch bin phi ph hp, thun li v nhanh chng Phi tch c trit tinh du trong nguyn liu, tn tht tinh du
trong qu trnh ch bin cng thp cng tt.

Chi ph u t vo sn xut l t nht

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Cc phng php sn xut tinh du

Faculty of Applied Chemistry

Nhng phng phap khai thac tinh du nh:

Phng php ha l (chng ct v trch ly) Phng php c hc Phng php kt hp (ha l v c hc hoc sinh ha (ln
men) v c hc, hoc sinh ha v ha l...

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Cc phng php sn xut tinh du

Phng phap chng ct

Faculty of Applied Chemistry

Phng php chng ct bng nc

Nguyn liu Xay nhuyn Bnh chng ct dietyl ete

H thng chng ct Chng ct Tinh du v nc

Tinh du

Bnh lng

Chit
Nc

Tinh du sn phm

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17

Cc phng php sn xut tinh du

Phng php chng ct bng hi nc

Faculty of Applied Chemistry

Yu cu: hi nc khng qu
nng v qu m. (qu nng s lm kh hay phn hy nguyn liu, qu m s gy hin tng ngng t).

Quy trnh chng ct tinh du bng hi nc

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Cc phng php sn xut tinh du

Phng phap chng ct u im: - Quy trnh k thut tng i n gin. - Thit b gn, d ch to. - Thi gian tng i nhanh.

Faculty of Applied Chemistry

- Khng i hi vt liu ph nh cc phng php tm trch, hp th.

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19

Cc phng php sn xut tinh du

Phng phap chng ct
Khuyt im:

Faculty of Applied Chemistry

- Khng c li i vi nhng nguyn liu c hm lng tinh du thp. - Cht lng tinh du c th b nh hng nu trong tinh du c nhng

cu phn d b phn hy.

- Khng ly c cc loi nha v sp c trong nguyn liu ( l nhng cht nh hng thin nhin rt c gi tr). - Trong nc chng lun lun c mt lng tinh du tng i ln. - Nhng tinh du c nhit si cao thng cho hiu sut rt km.
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Cc phng php sn xut tinh du

Phng phap tm trch

Faculty of Applied Chemistry

Tm trch bng dung mi d bay hi, da trn hin tng thm thu, khuch tn v ha tan ca tinh du c trong cc m cy i vi cc dung mi hu c
Yu t quan trng nht cho s thnh cng ca phng php ny l phm cht v c tnh ca dung mi s dng: - Ha tan hon ton v nhanh chng cc cu phn c mi thm trong nguyn liu. - Khng c tc dng ha hc vi tinh du. - Khng bin cht khi s dng li nhiu ln. - Hon ton tinh khit, khng c mi l, khng c, khng n mn thit b.

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Cc phng php sn xut tinh du

Phng phap tm trch
Nguyn liu X l Ngm trong dung mi

Faculty of Applied Chemistry

u im: Sn phm thu c theo phng php ny thng c mi thm t nhin. Hiu sut sn

Lc
Dung mi thch hp Tch sp thm

phm thu c thng cao hn cc phng

php khc. Khuyt im:

- Yu cu cao v thit b
Tinh du v dung mi Ct tch dung mi Tinh du thnh phm
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- Tht thot dung mi - Quy trnh tng i phc tp.

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Cc phng php sn xut tinh du

Faculty of Applied Chemistry

Ly trch tinh du di s h tr ca vi sng Di tc dng ca vi sng, nc trong cc t bo thc vt b nng ln, p sut bn trong tng t ngt lm cc m cha tinh du b v ra. Tinh du thot ra bn ngoi, li cun theo hi nc sang h thng ngng t (phng php chng ct hi nc) hoc ha tan vo dung mi hu c ang bao ph bn ngoi nguyn liu (phng php tm trch).

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Cc phng php sn xut tinh du

Tach tinh du bng phng phap c hc

Faculty of Applied Chemistry

Phng php ny ch yu dng tch tinh du trong cc loi v

qu nh cam, chanh, qut....

Tch tinh du bng phng phap hp ph

Phng php hp ph thng s dng tch tinh du ca cc loi

hoa, c bit l cc loi hoa c kh nng sinh thm tinh du dng

kh sau khi thu hi khi cy nh hoa nhi, hoa hu...

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nh tnh menthol
Phng phap sc k lp mng

Faculty of Applied Chemistry

Bnh khai trin s dng hn hp dung mi ethylacetate (5%) v

toluen (95%)

Thuc th: ho tan 1g vanilline trong 100 ml ethanol 950,

trn nc . Sau cho vo 2 ml H2SO4 m c.

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nh lng menthol

Faculty of Applied Chemistry

nh lng menthol

Menthol este ha Menthol ton phn

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NH LNG MENTHOL ESTE HA

Faculty of Applied Chemistry

Tinh du (2g)

3ml ethanol 96% Trung ha bng dd KOH 0.5N trong ethanol 96% 10 git phenolphthalein 2% trong ethanol 96%

X phng ha

20ml dd KOH 0.5N trong ethanol 96%

un cch thy

60 pht c lp sinh hn ngc ngui Pha long vi 60ml nc ct

Chun
8/8/2012

Dung dch HCl 0.5N n khi mt mu

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NH LNG MENTHOL ESTE HA

Faculty of Applied Chemistry

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NH LNG MENTHOL TON PHN

Faculty of Applied Chemistry

Tinh du (10ml) Acetyl ha

20ml Anhydride acetic 2g Sodium acetate un 60 pht, ngui Thm 50ml nc ct un, lc cch thy 15 pht, ngui

Kh anhydride
Loi nc

Ra tinh du
Tinh du acetyl ha
8/8/2012

50ml dd chloride 20ml nc ct Lm khan bng Na2SO4, lc

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NH LNG MENTHOL TON PHN

Faculty of Applied Chemistry

Tinh du acetyl ha (2g)

3ml ethanol 96% Trung ha bng dd KOH 0.5N trong ethanol 96% 10 git phenolphthalein 2% trong ethanol 96%

X phng ha

20ml dd KOH 0.5N trong ethanol 96%

un cch thy

60 pht c lp sinh hn ngc ngui Pha long vi 60ml nc ct

Chun
8/8/2012

Dung dch HCl 0.5N n khi mt mu

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NH LNG MENTHOL TON PHN

Faculty of Applied Chemistry

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THC NGHIM
Nguyn liu Xay nhuyn Bnh chng ct dietyl ete

Faculty of Applied Chemistry

H thng chng ct Chng ct Tinh du v nc

Tinh du

Bnh lng

Chit

Tinh du sn phm

Nc
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THC NGHIM
Phng php chng ct bng nc

Faculty of Applied Chemistry

Quy trnh chng ct tinh du bng nc

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THC NGHIM

Faculty of Applied Chemistry

S thay i ap sut v lng nc qua cac ln ly trch

Thng s Ln Ln 1 Khi p sut (mbar) (g) 300 400 85 Nhit (0C) Lng Tc Thi gian

lng

nc
(ml) 300

quay
(rpm) 50

un
(h) 2

Ln 2
Ln 3 Ln 4 Ln 5

300
300 300 300

400
400 500 700

85
85 85 85

250
200 300 300

50
50 50 50

2
2 2 2

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KT QU

Faculty of Applied Chemistry

Thng s Ln Ln 1 Ln 2 Ln 3

Khi lng (g) 300 300 300

p sut (mbar) 400 400 400

Nhit (0C) 85 85 85

Lng nc (ml) 300 250 200

Tc quay (rpm) 50 50 50

Thi gian un (h) 2 2 2

Kt qu (ml) 3 3 3

Ln 4
Ln 5

300
300

500
700

85
85

250
250

50
50

2
2

3
0

Ch : sn phm tinh du thu c cha tin hnh thu hi dung mi.

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KT LUN

Faculty of Applied Chemistry

S dng phng php sc k lp mng khng thu c kt qu

Hm lng tinh du trong cy hng cy rt thp Phng php chng ct bng nc khng th p dng cho vic
ly trch tinh du t nhng nguyn liu hm lng tinh du thp (cy hng cy).

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Ti liu tham kho

Faculty of Applied Chemistry

[1] Tt Li, Nhng cy thuc v v thuc Vit Nam, Nh Xut Bn Y Hc 2004.

[2] L Ngc Thch, Tinh du, NXB HQG TP H Ch Minh 2003. [3] Nguyn B, Giao trnh thc vt hc, NXB Gio dc H Ni 2007. [4] V Th Ngc M (1999), lun vn thc s Khao sat tinh du mt s loi cy ging Mentha trng min Nam Vit Nam h Hoa mi (Lamiaceae), Trng i hc khoa hc t nhin HQG TPHCM. [5] Ci ngum thc phm. Hng cy. http://coinguonthucpham.com/node/64 (cp nht ln cui ngy 18/7/2012).

Tn tc gi

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Faculty of Applied Chemistry

Chn thnh cm n !!!!!