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vn
Nguyn l ca k thut di tru n uyn ngc c Re everse genetics (k thu di truy ngc) l mt thu ng c s dng t ut n l t c trong ngnh virus hc ph t ch vic to r virus t cDNA tch dng. H thng di tr hn h ra h ruyn u g s nm 1978. Khc vi v virus ngc tin c p dng cho virus RNA si dng t n RNA s dng, bn thn c gen ca virus RNA si m k i b c A khng c k nng t ly kh nhim. D vic phin m v ti bn v Do v virus si m i hi s ng biu hin ca p m u phc h polym merase viru v s to thnh phc h gia RN v nucl us c NA leoprotein. ` N 1994, Conzelmann v cng s vi virus di ti t h m , s hp, ln tin ch u hng minh kh to ra virus RN si m h c NA hon ton t cDNA tc dng. Y t quyt nh ch u thnh c ny l s tng hp (+) antige ng s p enome t cD DNA kh hng bt cp vi (+)mR p RNA m ha c protein L, N, P, v th khng cn tr qu trnh ti b virus. cho n n

Hnh 1: Quy trnh t ra virus R o RNA si m khng phn on. T b c ng nui c vi n bo y nhng pla asmid biu hin protein L, N, P, v nhng pla n asmid cha antigenome ca e virus di, di s i khin ca T7 RNA polymerase p u a promoter. B c cui c l ng m p p ly nhim t bo vi virus vaccinia ti t hp cung c T7 polymerase, khi ng qu trnh ti bn virus s

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S qui trnh ti thit virus ti t hp bng k thut di truyn ngc

Hnh tri: 8 plasmid bao gm 6 plasmid mang gen ca chng virus nhc c, 1 plasmid mang gen N1 nguyn ven v 1 plasmid mang ggen H5 bin i c nhim bin vo t bo 293T v MDCK. Th virus mi c ti thit hon chnh v nhn ln nh t bo dung hp gia 293T v MDCK. Hnh phi: Nhn th virus ti thit bng trng c phi 10 ngy tui. Kt qu thu c th virus ti t hp vi nhng c im mi nh sau: (1) Mang 2 gen ca chng virus c; (2) Mang tnh nhc c do b loi 4 amino acid kim; (3) Cho php nhn nhanh trong trng c phi.

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K K thut di tr ruyn ngc l nh du mt bc n c u nhy vt tron s hiu bit virus sinh hc ng h ph t v virus gy bn v pht t hn nh trin vaccine hiu qu c e chng li nh hiu cn bn do nh virus ng v v gia cm t m Gip pht tri thuc ch nga c hiu qu v cm gia c ngn c G n ng i m chn i dch h C C th ni vi s dng k thut di truyn ng l mt h ic c ng i hp l to c p chng virus phc v cho sn xu vacxin cm gia cm v tc nh t vi hanh, p dn c trn dng ng t bo cng nhn p dng vi mi chng virus gy bnh th c n, c i s huc cc sub btype kh nhau cng b, loi b on c trn gene H trnh s tp n hc c c c HA, c nhim

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(virus hoang di t dng t bo khng chun hoc virus cha cc c t khc).

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