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I. Nguyn tc: - Tt c cc dng nito c trong c th hay cc m c gi l nito tng s. Nito c trong thnh phn amino acid ca protein l nito protein. Nito khng c trong thnh phn protein nh ca cc mui v c, acid nitric, cc amino acid t do, cc peptid, ure v cc dn xut ure, purin v pirimidin l nito phi protein. Nito tng s = nito protein + nito phi protein - Trc tin, mu c v c ha bng H2SO4 c nhit cao v c cht xc tc. Phn ng xy ra nh sau: H2SO4 2H2O + 2SO2 + O2 - Oxi to thnh trong phn ng li oxy ha cc nguyn t khc. Cc phn t cha nito di tc dng ca H2SO4 li to thnh NH3. V d cc protein b thy phn thnh amino acid, C v H ca amino acid to thnh CO2 v H2O, cn N c gii phng di dng NH3 kt hp vi H2SO4 d to thnh (NH4)2SO4. Tan trong dung dch 2NH3 + H2SO4 (NH4)2SO4 - Cc nguyn t P, K, Ca, Mg chuyn thnh dng oxid: P2O5, K2O, CaO, MgO, - ui amoniac ra khi dung dch bng NaOH (NH4)2SO4 + 2NaOH Na2SO4 + H2O + 2NH3 - NH3 bay ra cng vi nc sang bnh hng, bnh hng cha H3BO3 2NH4OH + 4H3BO3 = (NH4)2B4O7 + H2O Hoc H2SO4, phn ng xy ra nh sau: 2NH4OH + H2SO4 (NH4)2SO4 + 2H2O 2. Cch thc hin: Thc hin ln lt cc thao tc: - V c ha mu - Ra my - Chun b bnh hng - Li cun hi nc v Chun S khi c th cc bc
Mu K2SO4 + CuSO4 (9:1)
H2SO4, 5ml
V c ha mu
- Ra my
M nc lm lnh un si bnh A Cho nc ct vo phu
Hi nc
Ngng t erlen F
5ml nc erlen
Ngng un A
Nc t C B
Ht nc C
Thm nc vo phu, m kp P1 Nc C
Nc rt qua B
Nc B y m kp P1
M kp P1 dd C
Trng phu
M kp dd C
Dd trong erlen F
Kt thc chun
- H s hiu chnh x c tnh theo cng thc X= - Gi Vo tr s trung bnh ca 2 ln th khng V1 tr s trung bnh ca 3 ln th tht Vy V = Vo Vt l lng NaOH tng ng vi NH3 phng thch bi 10ml dd m v c ha v pha long. S mol amoniac phng thch tng ng vi s mol NaOH, Do , s mol amoniac phng thch bi 10ml dd m v c ha v pha long l mol - S gram m c trong 10ml dd m v c ha v pha long l: 14. V.X.10-5 (g) - S gam m c trong 100ml dd m v c ha l: 14. V.X.10-5. - Nu nguyn liu l cht rn: %N = 14. V.X.10-4. - Nu nguyn liu l cht lng: g N/l = 14. V.X.10-4. ; Vmau th tch mu em v c ha ; m l lng mu em xc nh m = 14. V.X.10-4 (g)
M rng: xc nh hm lng nito tng s, ngoi phng php Kjehdahl, cn c phng php so mu, s dng thuc th Nessler Nguyn tc: - Khi ta cho tc dng vi mt dung dch mui amonium (NH4+) vi thuc th Nessler ( dung dch mui ca Hg v K vi Iodur) s to thnh mt phc cht c mu vng. Cng ca mu ny l mt hm s tuyn tnh vi nng ca mui amonium, v th ta c th dng phng php so mu xc nh hm lng amonium c trong mu