PHNG PHP PHN TCH PH HUZNH QUANG V NG DNG TRONG CNG NGH THC PHM

GVHD: NGUYN KHC KIM

NI DUNG CHNH
PP PHN TCH PH HUZNH QUANG CC YU T NH HNG NG DNG

S PHT X HUZNH QUANG

trng thi kch thch, phn t c th tr v trng thi in t c bn v pht ra nng lng di dng bc x, bc x huznh quang c bc sng ln hn so vi cc bc x c hp thu trc Phng php huznh quang l phng php xc nh bn cht v nng ca mt cht nh phn tch bc sng v cng ca bc x huznh quang.

PP PHN TCH PH HUZNH QUANG

S TO THNH PH HUZNH QUANG

 ng pha di th hin trng thi c bn nng lng ca phn t, tng ng vi trng thi n c k hiu l S0. ng m nt pha trn l trng thi kch thch nng lng ca phn t tng ng vi cc trng thi n S1 v S2 . Cc vch mng pha di v pha trn ng vi s phc hi dao ng.

 Phn t b kch thch c th b hp thu nng lng ca 2 di bc x 1 v 2 ng vi bc chuyn S0 S1 va S0 S2 Sau xy ra qu trnh hi phc dao ng v trng thi S1 ri pht huznh quang(bc x 3) tr v trng thi c bn.

Qu trnh kh hot
phn t c th b kch thch n mt mc dao ng bt k trong qu trnh kch thch e nng lng d b mt i tc thi khi c s va chm gia phn t b kch thch v phn t dung mi => s pht x huznh quang trong dung dch xy ra

NGUYN TC NG DNG PH HUZNH QUANG TRONG PHN TCH

Phn tch nh tnh (Dng quang ph so snh) Phn tch bn nh lng Phn tch nh lng: xc nh nng ca cc cht trong dung dch

CC YU T NH HNG
Bc sng nh sng kch thch Nng pH v dung mi Nhit Tp cht Cu to phn t v s pht quang huznh quang Lng oxi khng ha tan

CC B PHN MY O

Hnh s khi thit b o huznh quang

CC KIU T CUVET
Vung gc vi tia ti Nghing 37 so vi tia ti Cuvet quay

Nguyn l{ hot ng

s ph k huznh quang

Nguyn l{ hot ng ph k huznh quang

Bc x pht ra t ngun sng n xenon 1 i qua b n sc kch thch 2 n mu o 3 pht ra bc x huznh quang . Trn ng i mt phn nh sng i qua cuvet so snh 4. Chm bc x huznh quang ny qua gng cu lm 5 i n b n sc pht x 6, sau i n ng nhn quang 7 (etect) ri sang b phn c tn hiu v ghi nhn kt qu 8.

Bng so snh s khc nhau gia ph k huznh quang v mt s loi ph k khc

Phng php ph k hunh quang Dng c Huznh quang k v ph k huznh o quang Ngun -Cng mnh sng - n h quang thu ngn pht ra cc vch ph 254, 366, 405, 436, 546, 577, 691, 773 nm

Phng php ph kch thch Phng php ph hng ngoi electron Ph k t ngoi v kh kin Ph k hng ngoi - n teri cho vng t ngoi -n Nernst: l ng di 2-5cm, ( 200 350 nm) 1-3mm cha oxit kim loi t him - n tungsen cho vng kh kin ( 350 1000 nm) -n Globar:di 40-60mm, 46mm lm t silic cacbit

- n xenon pht bc x lin tc t 300-1300nm -C 2 u pht tia hng ngoi B phn -knh lc hp th v knh lc giao - lng knh Littrow bng thch -H thng tch nh sng n anh sc gm lng knh hoc cch t chn sng thoa - cch t l nhng tm thu -dng LiF, CaF2, NaCl... ch to tinh c vch nhng ng lng knh cho tia hng ngoi -Thit b lun c 2 b phn chn song song (300-3600 sng -cch t l nhng tm thu tinh vch/mm) c vch nhng ng song -Thit b ch c 1 b tch n song(20-300vch/mm) sc -ng nhn quang c cng cao -ng nhn quang -bin tn hiu nh sng thnh Detect tn hiu in - dng etect dn diot cho ph -photodiot -nh b phn khuch i m k huznh quang -dn diot dng in mnh ln nhiu ln truyn sang b phn t ghi Cuvet mu -hnh tr trn hoc hnh tr ch -lng knh v ca s cuvet u -cuvet c cc tm ca s lm t nht lm bng thch anh tinh th KBr hay NaCl -lm bng thu tinh hoc silic oxit -cch t

 Ngoi ra, s sp xp v tr ngun sng v mu o gia cc loi cng c s khc bit. kiu (I) - ph k huznh quang b n sc c t vung gc vi hng i ca ngun sng ban u v nh sng i qua b n sc l nh sng huznh quang t mu pht ra kiu (II) ph k t ngoi kh kin ngun sng i qua mu o ri i thng vo b n sc. kiu (III) ph k pht x - ngun sng trc tip t mu ri sau i vo b n sc.

NG DNG
TRONG DC PHM:
Xc nh hm lng quinin trong dc t (mt h ln cc dc cht c hi cho sc khe)

NG DNG V D: Xc nh LA-mt loi cht gy o gic

Ly mu: kim ha nh mu huyt tng hay nc tiu v chit bng hn hp n - heptan v ru Bc 1: izoamylic trong t s th tch 98:2LA
X l mu: chit LA vo pha hu c v ti chit n bng dung dch nc ca HCl 0,004M Bc 2: o trc tip huznh quang ca mi dch ti chit axit dung bc sng kch thch - 335nm v bc sng Bc 3: huznh quang - 435nm.

NG DNG
TRONG NGNH MI TRNG: Xc nh nng cht c hi trong c cht gy ung th trong khng kh

NG DNG Xc nh cht Benzipiren

Bc 1:

Cho mu khng kh qua dung dch ca axit sunfuric Phn tch tip bng php o ph huznh quang, bc sng kch thch bng 520nm bc sng huznh quang 545nm

Bc 2:

NG DNG
TRONG Y HC: Xc nh hm lng ion v c trong mu v nc tiu.

NG DNG
Ly mu huyt tng ca mu hoc nc tiu. Cho to phc vi dung dch m (pH=6,5) ca 8 Oxyquinolin phc chelat. o hunh quang ca phc chelat nhn c 510nm khi kch thch bng bc x c bc sng 380 nm.

ng Dng
Trong cng ngh thc phm (TP): Ph huznh quang nguyn t : Phn tch hm lng thy ngn trong TP nh c, ung, ng cc, phn tch vt trong TP do gii hn xc nh tuyt vi v di o rng. Ph huznh quang tia X: Phn tch cc nguyn t ch yu, th yu, vi lng v nhng cht c hi trong TP.

ng Dng

V D Xt nghim vi khun E.coli nhm ngn chn nhim c thc phm:

To phc khng th v ht nano silica pht quang pht hin nhanh VK gy bnh pht hin cc on ADN ca cc loi VK gy bnh trong TP

Kim nghim TP bng tia hunh quang DNAzymes

C ch xc nh vi khun vi khun gy bnh

Bc 1 To c phc hp khng th c hiu + ht nano silica. Bc 2 Tin hnh k thut min dch huznh quang. Bc 3 Xc nh s lng vi khun (VK) ch bng ng chun gia cng pht quang ca VK gn ht nano v s lng VK trong dung dch chun.

nh huznh quang: Phc hp ht nano silica khng th; VK gn vi phc hp khng th - Silica; VK gn vi phc hp khng th - QDs

QUAN ST V M TRC TIP CAC T BAO VI KHUN PHT QUANG DI KNH HIN VI HUYNH QUANG

Ph huznh quang ca phc hp ht silica khng th gn kt vi vi khun E. coli O157:H7.

y l c s xy dng ng chun: cng ph - s lng E.coli O157:H7 pht hin nhanh, nhy s lng vi khun ny mt thp

Phn tch lng d CBM (thuc bo v thc vt trong thc phm) bng phng php sc k{ lng cao p (HPLC) u d huznh quang (FD)
Carbamate (CBM) l nhm thuc bo v thc vt rt ph bin c cng thc chung : O || R1NH - C - OR2 Trong R1 v R2 l aryl hoc ankyl.

Thc hin qu trnh

SP l dn xut HQ 1hydroxytylthio 2 metylisoindol c cc i hp thu khong 340 nm v cc i pht x 440-460 nm. Methyl amin+ thuc th O phthalaldehyde (OPA) v 2 mercaptoethanol Thy phn trong mi trng baz sinh ra methyl amin CBM tch trn ct sc k pha o

HT
CHN THNH CM N THY V CC BN LNG NGHE