CCH GII NHANH BI TON THY PHN PEPTIT

CCH GII NHANH BI TON THY PHN PEPTIT

PHN I: IM C S. a. Thy phn hon ton: H[NHRCO]nOH + (n-1) H2O nH2NRCOOH. b. Thy phn khng hon ton: Th d: H[NH(CH2)2CO]4OH + H2O H[NH(CH2)2CO]3OH+ H[NH(CH2)2CO]2OH+ H2N(CH2)2COOH Cch 1: Vi phn ng trn khi cho bit s mol cc cht sau phn ng ,th ta da vo s mol ri cn bng phn ng s tnh c s mol peptit ban u tham gia phn ng v suy ra khi lng.
23,75 H[NH(CH2)2CO]4OH + H2O 9H[NH(CH2)2CO]3OH+ 8H[NH(CH2)2CO]2OH+ 52H2N(CH2)2COOH 0,475 mol 0,18 mol 0.16 mol 1,04 mol

1. Phn ng thy phn ca Peptit:

Khi lng ca Peptit l: 0,475(89x4- 3x18) = 143,45(gam) Cch 2: gii nhanh hn, ta lm nh sau: t Peptit H[NH(CH2)2CO]4OH bng Cng thc gn: (X)4 ( Vi X = [NH(CH2)2CO] Ta ghi phn ng nh sau: Hoc ghi : (X)4 0,18 mol 2. Cch gii : *p dng LBTKL tnh lng nc khi bit khi lng Peptit phn ng v khi lng cht sinh ra. *p dng LBTKL tnh c lng mui khi cho Aminoacid sinh ra tc dng vi HCl,H 2SO4. * Khi tinh ton nn tnh theo cch 2 trn. 3. Tnh nhanh khi lng Mol ca Peptit: * Th d: H[NHCH2CO]4OH . H[NHCH(CH3)CO]3OH H[NHCH2CO]nOH . Ta c M= MGli x 4 3x18 = 246g/mol Ta c M= MAla x 3 2x18 = 231g/mol Ta c M= [MGli x n (n-1).18]g/mol (X)3 + X ; (X)4 2 (X)2 0,16mol v (X)4 0,215mol 4X 0,86 mol 23,75 (X)4 + H2O 9 (X)3 + 8(X)2 + 52X

0.18 mol 0,18 mol 0,08mol

T 3 phn ng ta tnh c s mol ca Peptit ban u tham gia phn ng l:(0.18+0.08+0,215)mol

* i vi 2 Peptit khi thy phn c t l s mol bng nhau,th ta xem 2 Peptit l mt Peptit v ghi phn ng ta nn ghi gp . Khi lng mol ca Petpti chnh l tng khi lng mol ca 2 Peptit . Th d: Tripeptit H[NHCH2CO]3OH v Tetrapeptit H[NHCH2CO]4OH (c s mol bng nhau) Th ta xem 2 Peptit l Heptapeptit: H[NHCH2CO]7OH v M= 435g/mol
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CCH GII NHANH BI TON THY PHN PEPTIT

4. Phn ng chy ca Peptit: * Th d: Tripeptit mch h X v Tetrapeptit mch h Y c to t mt Aminoacid no,h trong phn t c 1(-NH2) + 1(-COOH). t chy X v Y. Vy lm th no t CTPT cho X,Y? Ta lm nh sau: T CTPT ca Aminoacid no 3 CnH2n+1O2N 2H2O thnh CT C3nH6n 1O4N3(y l cng thcTripeptit) V 4 CnH2n+1O2N 3H2O thnh CT C4nH8n 2O5N4(y l cng thcTetrapeptit) ...... Nu t chy lin quan n lng nc v cacbonic th ta ch cn cn bng C,H tnh ton cho nhanh. C3nH6n 1O4N3 + pO2 C4nH8n 2 O5N4 + pO2 PHN II: 3nCO2 + (3n-0,5)H2O + N2 4nCO2 + (4n-1)H2O +N2.Tnh p(O2) dng BT nguyn t Oxi?

HNG DN GII CC BI TON MIMH HA:

Bi 1: X l mt Tetrapeptit cu to t Aminoacid A,trong phn t A c 1(-NH2) + 1(-COOH) ,no,mch h.Trong A Oxi chim 42,67% khi lng . Thy phn m gam X trong mi trng acid th thu c 28,35(g) trpeptit; 79,2(g) ipeptit v 101,25(g) A. Gi tr ca m l? a. 184,5. b. 258,3. c. 405,9. d. 202,95. Hng dn: T % khi lng Oxi trong A ta xc nh c A l Gli ( H2NCH2COOH) vi M=75 Cng thc ca Tetrapeptit l H[NHCH2CO]4OH vi M= 75x4 3x18 = 246g/mol Tnh s mol: Tripeptit l : 28,35: 189 = 0,15(mol) ipeptit l : 79,2 : 132 = 0,6 (mol) Gii gn nh sau: t mt xch NHCH2CO = X. Ghi s phn ng : (X)4 0,15 (X)3 + 0,15 X ; 0,15 (X)4 0,3 2 (X)2 0,6 v (X)4 0,3 4X 1,2 Glyxin(A) : 101,25 : 75 = 1,35(mol).

T s trn ta tnh c: S mol X phn ng l: (0,15+0,3+0,3)=0,75mol m = 0,75.246 =184,5(g) Bi 2: Thy phn hon ton 143,45 gam hn hp A gm hai tetrapeptit thu c 159,74 gam hn hp X gm cc Aminoacid (Cc Aminoacid ch cha 1nhosm COOH v 1 nhm NH 2 ) . Cho tan b X tc dng vi dung dch HCl d,sau c cn dung dch th nhn c m(gam) mui khan. Tnh khi lng nc phn ng v gi tr ca m ln lt bng? a. 8,145(g) v 203,78(g). b. 32,58(g) v 10,15(g). 203,78(g). Hng dn: t Cng thc chung cho hn hp A l H[NHRCO]4OH
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c. 16,2(g) v 203,78(g)

d. 16,29(g) v

CCH GII NHANH BI TON THY PHN PEPTIT

Ta c phn ng : H[NHRCO]4OH + 3H2O Hay: (X)4 + 3H2O 4X 4 H2NRCOOH ( Trong X = HNRCO)

p dng LBTKL nH2O = T phn ng nX= n H2O =

4 3

mX mA 0,905(mol) mH2O = 16,29 gam. 18 4 .0,905(mol ) 3

Phn ng ca X tc dng vi HCl :

X + HCl

X.HCl
4 .0,905(mol ) .36,5 = 203,78(g) 3

p dng BTKL m(Mui) = mX + mHCl = 159,74 +

Bi 3: Tripeptit M v Tetrapeptit Q c to ra t mt Aminoacid X mch h ( phn t ch cha 1 nhm NH2 ) .Phn trm khi lng Nito trong X bng 18,667%. Thy phn khng hon ton m(g) hn hp M,Q(c t l s mol 1:1) trong mi trng Acid thu c 0,945(g) M; 4,62(g) ipeptit v 3,75 (g) X.Gi tr ca m? a. 4,1945(g). b. 8,389(g). c. 12,58(g). d. 25,167(g).

Hng dn: Ta c %N =

14 18,667 MX 75 X l Glyxin MX 100

Do hai peptit c t l s mol phn ng 1:1 nn xem hn hp M,Q l mt Heptapeptit : H[NHCH2CO]7OH V c M = 435g/mol. Ghi phn ng :
27 (Gli)7 + H2O 7 27 0,005mol 7

(Gli)3 0,005mol

7 (Gli)2 0.035mol

+ 10 (Gli) 0.05mol

m(M,Q) =

27 0,005mol.435 = 8,389(g) 7

Gii theo cch khc: (Gli)7 0,0025mol 2(Gli)3 + 0,005mol Gli ; (Gli)7 0,0025 0,035/3 3 (Gli)2 + Gli v (Gli)7 0,0358/7 7(Gli) 0.0358

0,035mol 0,035/3

T cc phn ng tnh c s mol ca (Gli)7 l : 0.01928(mol) Bi 4: Tripeptit mch h X v Tetrapeptit mch h Y u c to ra t mt Aminoacid no,mch h c 1 nhm COOH v 1 nhm NH2 .t chy hon ton 0,1 mol X thu c sn phm gm H2O,CO2 v N2 trong tng khi lng CO2 v H2O bng 36,3(g) .Nu t chy hon ton 0,2 mol Y th s mol O2 cn phn ng l? a. 2,8(mol). b. 1,8(mol). c. 1,875(mol). d. 3,375 (mol) Hng dn: R rng X,Y u sinh ra do Aminoacid c CT CnH2n+1O2N. Do vy ta c CT ca X,Y tng ng l: C3nH6n 1O4N3(X) , C4nH8n 2O5N4(Y).
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CCH GII NHANH BI TON THY PHN PEPTIT

Phn ng chy X: C3nH6n 1O4N3 + 0,1mol Phn ng chy Y: C4nH8n 2 O5N4 + 0,2mol pO2 3nCO2 + (3n-0,5)H2O + N2

0,3n(mol) 0,3(3n-0,5)mol

Ta c phng trnh tng khi lng H2O v CO2 : 0,3[44.n + 18. (3n-0,5)] = 36.3 n = 2 pO2 0,2.p 4nCO2 + (4n-1)H2O + N2 . 0,8n (0,8n -0,2)

p dng BT nguyn t Oxi : 0,2.5+ 0,2.2p = 0,8.2.2 +(0,8.2 -0,2) p = 9. nO2 = 9x0,2 = 1,8(mol) PHN III: BI TP T GII QUYT. Bi 5: ( H-2011) Thy phn hon ton 60(g) hn hp hai ipeptit thu c 63,6(g) hn hp X gm cc Aminoacid no mch h (H2NRCOOOH). Nu ly 1/10 hn hp X tc dng vi dung dch HCl d thu c m(g) mui. Gi tr ca m l? a. 7,82. b. 8,72. c. 7,09. d.16,3. Bi 6: ( H-2011) Thy phn ht m(g) Tetrapeptit Ala-Ala-Ala-Ala thu c hn hp gm 28,48(g) Ala ; 32(g) Ala-Ala v 27,72(g) Ala-Ala-Ala. Gi tr ca m? a. 66,44. b. 111,74. c. 81,54. d. 90,6. Bi 7: X l mt Hexapeptit cu to t mt Aminoacid H2N-CnH2n-COOH(Y) . Y c tng % khi lng Oxi v Nito l 61,33%. Thy phn ht m(g) X trong mi trng acid thu c 30,3(g) petapeptit, 19,8(g) ieptit v 37,5(g) Y. Gi tr ca m l? a. 69 gam. B. 84 gam. c. 100 gam. d.78 gam. Bi 8: Thy phn hon ton 1 mol Pentapeptit(X) thu c 3 mol Gli; 1 mol Ala; 1 mol Phe. Khi thy phn khng hon ton (X) thu c hn hp gm Ala-Gli ; Gli-Ala v khng thy to ra Phe-Gli. Xc nh CTCT ca Petapeptit? Hng dn: T cc ipeptit ta thy Gli gia Ala-Gli-Ala hoc Ala gia Gli-Ala-Gli. Nhng v thu c 1 mol Ala nn chc chn Ala phi gia Gli-Ala-Gli. Do khng c Phe-Gli to thnh nn Phe khng ng trc Gli m ng sau Gli. Vy CTCT l: Gli-Gli-Ala-Gli-Phe Bi 9: Thy phn 14(g) mt Polipeptit(X) vi hiu sut t 80%,thi thu c 14,04(g) mt aminoacid (Y). Xc nh Cng thc cu to ca Y? a. H2N(CH2)2COOH.b. H2NCH(CH3)COOH. Bi 10: Gi s trong phn t ch c 2 nguyn t S? a. 20.000(vC) Bi 11 ( H 2010) b.10.000(vC). c. 15.000(vC). d. 45.000(vC). ipeptit mch h X v Tripeptit mch h Y u c to ra t mt c. H2NCH2COOH d. H2NCH(C2H5)COOH Xc nh Phn t khi gn ng ca mt Polipeptit cha 0,32% S tromh phn t.

Aminoacid no,mch h c 1 nhm COOH v 1 nhm NH2 .t chy hon ton 0,1 mol Y thu c
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CCH GII NHANH BI TON THY PHN PEPTIT

sn phm gm H2O,CO2 v N2 trong tng khi lng CO2 v H2O bng 54,9(g) .Nu t chy hon ton 0,2 mol X,sn phm thu c cho loiij qua dung dch nc vi trong d th c m(g) kt ta . Gi tr ca m l? a. 45. b. 120. c.30. d.60. Bi 12: X l mt tetrapeptit cu to t mt amino axit (A) no, mch h c 1 nhm COOH ; 1 nhm NH2. Trong A %N = 15,73% (v khi lng). Thy phn m gam X trong mi trng axit thu c 41,58 gam tripeptit ; 25,6 gam ipeptit v 92,56 gam A. Gi tr ca m l : a. 149 gam. b. 161 gam. c. 143,45 gam. d. 159 gam. Bi 13: X v Y ln lt l cc tripeptit v tetrapeptit c to thnh t cng mt amino axit no mch h, c mt nhm COOH v mt nhm NH2. t chy hon ton 0,1 mol Y thu c sn phm gm CO2, H2O, N2, trong tng khi lng ca CO2 v H2O l 47,8 gam. Nu t chy hon ton 0,3 mol X cn bao nhiu mol O2 ? a. 2,8 mol. b. 2,025 mol. c. 3,375 mol. d. 1,875 mol. Bi 14: X l tetrapeptit Ala-Gly-Val-Ala, Y l tripeptit Val-Gly-Val. un nng m gam hn hp X v Y c t l s mol nX : nY = 1 : 3 vi 780 ml dung dch NaOH 1M (va ), sau khi phn ng kt thc thu c dung dch Z. C cn dung dch thu c 94,98 gam mui. m c gi tr l : a.. 68,1 gam. b. 64,86 gam. c. 77,04 gam. d. 65,13 gam. Bi 15: Mt hemoglobin (hng cu ca mu) cha 0,4% Fe (mi phn t hemoglobin ch cha 1 nguyn t Fe). Phn t khi gn ng ca hemoglobin trn l : a. 12000. Bi 16: b. 14000. c. 15000. d. 18000. un nng alanin thu c mt s peptit trong c peptit A c phn trm khi lng

nit l 18,54%. Khi lng phn t ca A l : a. 231. Bi 17: duy nht). X l : a. tripeptit. Bi 18: b. tetrapeptit. c. pentapeptit. d. ipeptit. Khi thy phn hon ton 65 gam mt peptit X thu c 22,25 gam alanin v 56,25 gam d. ipeptit. b. 160. c. 373. d. 302. Khi thy phn hon ton 55,95 gam mt peptit X thu c 66,75 gam alanin (amino axit

glyxin. X l : a. tripeptthu c. Bi 19: ca Z l : a. 103.

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b. tetrapeptit.

c. pentapeptit.

Thu phn hon ton 500 gam mt oligopeptit X (cha t 2 n 10 gc -amino axit)

thu c 178 gam amino axit Y v 412 gam amino axit Z. Bit phn t khi ca Y l 89. Phn t khi

b. 75.

c. 117.

d. 147.
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CCH GII NHANH BI TON THY PHN PEPTIT

Bi 20: Tripeptit X c cng thc sau :

H2NCH2CONHCH(CH3)CONHCH(CH3)COOH Thy phn hon ton 0,1 mol X trong 400 ml dung dch NaOH 1M. Khi lng cht rn thu c khi c cn dung dch sau phn ng l : a. 28,6 gam. Bi 21: b. 22,2 gam. c. 35,9 gam. d. 31,9 gam. Protein A c khi lng phn t l 50000 vC. Thu phn 100 gam A thu c

33,998 gam alanin. S mt xch alanin trong phn t A l : a. 191. Bi 22: b. 38,2. c. 2.3.1023 d. 561,8. Thy phn 1250 gam protein X thu c 425 gam alanin. Nu phn t khi ca X bng

100000 vC th s mt xch alanin c trong X l : a. 453. Bi 23: b. 382. Thu phn hon ton 1 mol pentapeptit A c. 328. d. 479. th thu c 3 mol glyxin ; 1 mol alanin v

1mol valin. Khi thu phn khng hon ton A th trong hn hp sn phm thy c cc ipeptit Ala-Gly ; Gly-Ala v tripeptit Gly-Gly-Val. Amino axit u N, amino axit u C pentapeptit A ln lt l : a. Gly, Val. Bi 24: b. Ala, Val. c. Gly, Gly. d. Ala, Gly. Thu phn khng hon ton tetrapeptit (X), ngoi cc a-amino axit cn thu c

cc ipetit: Gly-Ala ; Phe-Val ; Ala-Phe. Cu to no sau y l ng ca X ? a. Val-Phe-Gly-Ala. Bi 25: b. Ala-Val-Phe-Gly. c. Gly-Ala-Val-Phe. d. Gly-Ala-Phe-Val. Thy phn hon ton 1 mol pentapeptit X, thu c 2 mol glyxin (Gly), 1 mol alanin

(Ala), 1 mol valin (Val) v 1 mol phenylalanin (Phe). Thy phn khng hon ton X thu c ipeptit Val-Phe v tripeptit Gly-Ala-Val nhng khng thu c ipeptit Gly-Gly. ht X c cng thc l a. Gly-Phe-Gly-Ala-Val. c. Gly-Ala-Val-Phe-Gly. Bi 26: b. Gly-Ala-Val-Val-Phe. d. Val-Phe-Gly-Ala-Gly.

Cng thc no sau y ca pentapeptit (A) tha iu kin sau ?

Thy phn hon ton 1 mol A th thu c cc - amino axit l : 3 mol glyxin, 1 mol alanin, 1 mol valin. Thy phn khng hon ton A, ngoi thu c cc amino axit th cn thu c 2 ipeptit : Ala-Gly ; Gly-Ala v 1 tripeptit Gly-Gly-Val. a. Ala-Gly-Gly-Gly-Val. c. Gly-Ala-Gly-Gly-Val. Bi 27: Thu phn hp cht : b. Gly- Gly-Ala-Gly-Val. d. Gly-Ala-Gly-Val-Gly.

H2NCH2CONHCH(CH3)CONHCH(CH(CH3)2)CONHCH2CONHCH2COOH s thu c bao nhiu loi amino axit no sau y ?

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CCH GII NHANH BI TON THY PHN PEPTIT

a. 3. Bi 28: b. 4. c. 5. d. 2.

Thu phn hp cht : s thu c bao nhiu loi amino axit no sau y ?

H2NCH(CH3)CONHCH(CH(CH3)2)CONHCH(C2H5)CONHCH2CONHCH(C4H9)COOH. a. 2. b. 3. c. 4. d. 5. Bi 29: Cho 3 cht X,Y,Z vo 3 ng nghim cha sn Cu(OH)2 trong NaOH lc u v quan st th thy: Cht X thy xut hin mu tm, cht Y th Cu(OH)2 tan v c mu xanh nht, cht Z th Cu(OH)2 tan v c mu xanh thm. X, Y, Z ln lt l : a. H tinh bt, HCOOH, mantoz. c. Anbumin, C2H5COOH, glyxin. Bi 30: a. dd HCl. b. Cu(OH)2/OHb. Protein, CH3CHO, saccaroz. d. Lng trng trng, CH3COOH, glucoz.

Thuc th c dng phn bit Gly-Ala-Gly v Gly-Ala l : c. dd NaCl. d. dd NaOH.

Chc cc em hc tp hiu qu nht!

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