Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bi 1 XC NH HM LNG NITROGEN DIOXIDE TRONG KHNG KH BNG PHNG PHP GRIESS- SALTZMAN
I. NGUYN TC Nitrogen dioxide c hp th vo dung dch hp thu Griess- Saltman , NO2 chuyn thnh ion nitrit v ion ny tc dng vi amine to phc azo mu tm hng CCH THC TIN HNH a/ iu kin thi tit: Tri ma , c gi nh b/ Ly mu: Ngy ly mu: Sng ngy 13/12/2010 Thi gian ly mu: 2h (8h45 - 10h45) V tr ly mu : t thit b ly mu pha bn tri 10 m ca cng chnh trng H KHTN v cch mp ng Nguyn Vn C, qun 5 : 30cm. Cch ly mu: - Cho 10 ml dung dch hp thu Saltman vo impinger kh, sau dng giy bc qun xung quanh impinger trnh phn hy cht hp th bi nh sng . - Lp impinger vo ga , bt bm ht vi tc 0.5 l/pht, ly mu trong 2 gi. Thi gian ly mu di ngn c th t chn thng qua cch d on nng NO 2 c trong khng kh lc ly mu, bui sng nng s thp hn bui tra v chiu. - Sau 2h tt bm, tho impinger chuyn dung dch trong impinger sang bnh nh mc 25ml, trng impinger bng nc ct 2 ln. II. c/ Phn tch: Xy dng thang chun : Bnh nh mc 25 ml DD chun nitrit( 10g NO2/ml) ml DD hp thu (ml) NO2(g/ml) tng ng 0 1 2 3 4 5 0 0.2 0.4 0.6 0.8 1.0 nh mc n vch bng dd hp thu 0 0.08 0.16 0.24 0.32 0.4

Phn tch mu: nh mc bnh cha mu bng dung dch hp thu v tin hnh o mu cng vi dy chun bc sng 550 nm. Ch nn phn tch mu cng sm cng tt trnh mt mu do phn ng vi cht oxy ha trong khng kh. Nn tin hnh o mu v dy chun ng thi trnh sai s. III. KT QU THU C

1|P ag e- Bi 1

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139 Bnh nh mc 25 ml DD chun nitrit( 10g NO2/ml) ml DD hp thu (ml) NO2(g/ml) tng ng hp th A 0 0 1 0.1 2 0.2 3 0.4 4 0.6 5 0.8 6 1.0 M1 M2

nh mc n vch bng dd hp thu 0 0 0.04 0.009 0.08 0.07 0.16 0.129 0.24 0.185 0.32 0.23 0.40 0.292 0.038 0.042

Ta c phng trnh hi quy: A = 0.742C 0.0006 H s tng quan R2 = 0.9897 Mu 1: Nng NO2 c trong bnh nh mc 25 ml : C= -

0.052 g/mL

Hm lng NO2 trong mu kh thu c g NO2 = 0.052* 10 = 0.52(g)

Th tch khng kh ly (L) quy v iu kin 250C, 1atm + Vn tc ly mu: 0.5 lit/pht + thi gian ly mu: 120 pht th tch mu ly: V = 0.5 * 60 = 60 (L)
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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Nhit trung bnh khng kh: T = 280C p sut khng kh ti ni ly mu : P = 1atm Suy ra: Nng NO2 i sang n v ppmV
V0 P *V *T0 1*60*298 59.4( L) P0 * T 1*301

Nng NO2 c trong khng kh:

8.75

4.65 ppm
Mu 2 Nng NO2 trong bnh nh mc 25 ml: C= -

0.057 g/mL

Hm lng NO2 trong mu kh thu c g NO2 = 0.057* 10 = 0.57(g)

Th tch khng kh ly (L) quy v iu kin 250C, 1atm + Vn tc ly mu: 0.5 lit/pht + thi gian ly mu: 120 pht th tch mu ly: V = 0.5 * 60 = 60 (L) Nhit trung bnh khng kh: T = 280C p sut khng kh ti ni ly mu : P = 1atm

Suy ra: -

V0

P *V *T0 1*60*298 59.4( L) P0 * T 1*301

Nng NO2 c trong khng kh:

9.6

Nng NO2 i sang n v ppmV

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

NO2(ppm) =

5,1

Nhn xt: Mu ta thu trc cng trng ni c nhiu xe c qua li - l nguyn nhn chnh sinh ra NO2 (NO2 sinh ra t ng c t trong), hm lng NO2 khng qu cao do nh hng ca thi tit hm ly mu (tri ma). H s tng quan R2=0.9897, vi h s tng quan nh vy kt qu c m bo tin cy nh gi hm lng NO 2 c trong khng kh ti ni ly mu.

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bi 2 PHNG PHP XC NH SO2 TRONG KHNG KH

NGUYN TC

I.

SO2 trong khng kh c hp thu bng dung dch potassium tetrachloromercurate K2HgCl4 hnh thnh phc dichlorosulfnatomercurate (II) ([HgCl2SO3] 2-). Sau cho tc dng vi pararoaniline methylsulfonic acid. hp thu ca dung dch c o ti bc sng 548 nm. 2KCl + HgCl = 2K+ + [HgCl4]2SO2 + [ HgCl4]2- +H2O = [ HgCl2SO3]3- + 2H+ +2Cl[HgCl2SO3]2- + HCHO + 2H+ = HO-CH2-SO3H + HgCl2 HO-CH2-SO3H + C19H18N3Cl+ HCl = pararoaniline methylsulfonic acid( mu tm)

II.

CCH LM Chun li dung dch Na2SO3: cho vo erlen 250mL 20mL Iodine 0.01N, thm tip 10mL dung dch Na2SO3. y kn phn ng khong 5 pht. Sau chun li bng dung dch thiosulfate 0.01N n mu vng nht. Sau thm vi git ch th h tinh bt s xut hin mu xanh, chun n mt mu xanh. Tnh nng SO2 nh sau: SO2 (g/mL) = Trong : A: s mL thiosulfate 0.01N chun trng B:s mL thiosulfate 0.01N chun mu N: nng ng lng ca thiosulfate K: micro ng lng gam ca SO2, K=32030 Dung dch chun lm vic: ly chnh xc 5mL dung dch Na2SO3 chun b phn trn v nh mc thnh 100mL bng TCM 0.04M Ly mu: Mu kh c thu qua bnh hp thu cha 10mL dung dch hp thu TCM. Tc ly mu lit/pht, thi gian ly mu 120 pht. Mu khng di nh nng Mt Tri, trong v sau khi ly mu, che bnh bng giy nhm Mu c chuyn qua bnh nh mc 25mL, ly 5mL nc ct trng. Thm 1mL acid sulfamic phn ng 10 pht. Thm chnh xc 2mL formaldehyde 0.4% v 5mL thuc th pararoaniline. o mu bc sng 548nm sau 30 pht Song song , ta dng ng chun nh sau:
Thc hnh ha ng dng mi trng 2010

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Dd sulfite pha long(mL) Dd hp thu (mL) Acid sulfamic 0.6 % Formaldehyde 0.4 % Pararosaniline Tnh kt qu SO2 (mg/m3) =

0.00 1.00 2.00 10.00 9.00 8.00 1.00 1.00 1.00 yn 10 pht 2.00 2.00 2.00 5.00 5.00 5.00 o mu sau 30 pht

3.00 7.00 1.00 2.00 5.00

4.00 6.00 1.00 2.00 5.00

5.00 5.00 1.00 2.00 5.00

V0: th tch khng kh quy v ktc( 250C, 101.3kPa) P: p sut khng kh ni ly mu V: th tch mu kh T: nhit trung bnh ca khng kh trong thi gian ly mu Po: 101.3 kpa To: -298 oK III. KT QU THU C SO2 (g/mL)

310

Nng SO2 trong dung dch lm vic: SO2 (g/mL) = = 15,5

Nng SO2 xy dng dy chun: Bnh 1: SO2 (g/mL) = Bnh 2: SO2 (g/mL) = Bnh 3: SO2 (g/mL) = Bnh 4: SO2 (g/mL) = Bnh 5: SO2 (g/mL) = =0 = 0,62 = 1,24 = 1,86 = 2,48

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bnh 6: SO2 (g/mL) = ng chun: Bnh Nng SO2 (g/mL) hp thu quang A 1 0 0

= 3,1 6 3.1 1.164

2 0.62 0.261

3 1.24 0.446

4 1.86 0.7

5 2.48 0.839

Dy chun nng SO2 v hp thu A

1.4 1.2 1 0.8

hp thu A

0.6 0.4 0.2 0

0
y = 0.3598x + 0.0106 R = 0.9921

0.5

1.5

2.5

3.5

Nng SO2 (g/mL)

Phng trnh hi quy tuyn tnh gia nng SO2 v hp thu quang A A = 0,3598.C + 0,0106 Kt qu hp thu quang A o c l: 0,511 Vy t phng trnh trn, suy ra kt qu nng SO2 trong mu c nh mc l : C SO2 (g/mL) = = 1,39

Khi lng SO2 trong 10mL mu: M SO2 (g) =

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. 10= 34,75
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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Nhit ti thi im v v tr ly mu: 280C Tc ly mu: 2l/pht Gi s ly mu trong 15 pht V0 = = 29,3 (L)

Vy nng SO2 trong khng kh: SO2 = x 1000 = 1.186 (g/m3) = 1,186 (mg/m3)

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Thc hnh ha ng dng mi trng 2010

Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bi 3 XC NH NHU CU OXY SINH HA

GII THIU

I.

_ Nhu cu oxy sinh ha BOD (Biochemical Oxygen Demand): l lng oxy cn thit dng oxy ha cc cht hu c di tc dng ca vi sinh vt trong iu kin hiu kh. _ n v: mg/L Vi sinh vt _ Cht hu c + O 2 ngha mi trng: BOD c ngha biu th lng cht hu c trong nc c th b phn hy bi vi sinh vt. CO2 + H2O + t bo mi +

II.

PHNG PHP XC NH C 2 phng php xc nh BOD: phng php pha long (Dillution) v phng php p k (Manometric method) Trong phn thc hnh ny, chng ta ch tm hiu phng php pha long. Phng php pha long: da trn php o oxy ha tan DO

DO l lng oxy ho tan trong nc cn thit cho s h hp ca cc sinh vt nc (c, lng th, thu sinh, cn trng v.v...) thng c to ra do s ho tan t kh quyn hoc do quang hp ca to. V vy, DO l mt ch s quan trng nh gi s nhim nc ca cc thu vc. Nguyn tc: Trung ha mu nc cn phn tch v pha long nhng t l khc nhau bng nc pha long (l nc ct c b sung cc cht dinh dng nh N, K, Fe v bo ha oxy, c hoc khng c cht c ch s nitrat ha). nhit 200C trong thi gian 5 ngy, trong ti. Xc nh nng oxy ha tan trc v sau khi . T tnh c lng oxy tiu tn trong 1 lt nc, tc gi tr BOD. I. Nhng lu khi tin hnh th nghim: 1. Ly mu v bo qun mu: Khng cn bo qun mu nu phn tch ngay trong vng 2 gi.
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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bo qun mu nhit 40C nu phn tch trong vng 6 gi. Bo qun lnh th phi lm mu ln 200C trc khi phn tch. 2. Chun b nc pha long: thm mi 1ml dung dch m phosphate, MgSO4, CaCl2, FeCl3 v dung dch cy( nu cn) cho mi lt nc pha long, a v nhit 200C, sc khng kh trong khong 2-3 gi, DO t 7-8 mg/L. Nc pha long c dng trong vng 24 gi. 3. X l mu s b: pH 6.5-8.0. Ch hm lng clo d. III. TIN HNH XC NH MU i vi nc pha long: c pha sn nc pha long khong chai BOD. Cho c t vo chai BOD,t ln bn in, nhng u d o DO xc nh c DO1 . Ly c t ra, y nc pha long vo chai BOD, y kn nt, trnh to bt kh. em 5 ngy 200C xc nh DO5. i vi dung dch BOD chun: Do bit c c im, tnh cht, v tr ly mu, ta c th d on vic pha long mu vi mt nng thch hp. D on khong BOD t 100 600 (mg/L), thc hin 2 t l pha long 1% v 2%. T l pha long 1%: ly 10 ml mu nh mc ln thnh 1L bng 990 ml nc pha long. T l pha long 2%: ly 20 ml mu nh mc ln thnh 1L bng 980ml nc pha long. Tin hnh tng t, khang mu nc pha long vo 2 chai BOD (t l pha long 1% v 2%) Cho c t vo 2 chai, xc nh DO1 bng u o. Ly c t ra, y mu nc pha long ( 1% v 2%) vo 2 chai BOD, y kn nt, trnh to bt kh. em 2 chai 5 ngy 200C xc nh DO5. Sau 5 ngy, o DO5 ca nc pha long v mu nc pha long (1% v 2%) bng my o DO (c t v u d).

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

IV.

KT QU THU C Nc pha long 7.9 DO1 (mg/L) 6.9 DO5 (mg/L) Kt qu BOD c tnh theo cng thc: BOD = (DO1 ca mu pha long 1% - DO5 ca mu pha long 1%- DO1 ca nc pha long DO5 ca nc pha long x h s pha long = (8.0 4.7-7.9 6.9) x 100 = 230 (mg/L) ( H s pha long 1% = 1000/10 =100) BOD = (DO1 ca mu pha long 2% - DO5 ca mu pha long 2%- (DO1 ca nc pha long DO5 ca nc pha long) x h s pha long = (7.9 - 4-7.9 6.9 ) x 50 = 145 (mg/L) ( H s pha long 2% = 1000/20 = 50) 4.7 4 Mu nc pha long 1% 8.0 Mu nc pha long 2% 7.9

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bi 4 XC NH PHOSPHAT, PHOSPHO TNG TRONG NC

PHNG PHP

I.

Xc nh nng ca phosphat, phospho tng da vo phng php hp thu ph nguyn t UV-Vis bng cch dng ng chun

II.

NGUYN TC

Trong mi trng acid, cc orthophosphat (PO 43-, HPO42-, H2PO4- ) s phn ng vi Ammonium molybdate v kali antimonyl hnh thnh phc antimony phosphomolybdate, sau phc ny b kh bng acid ascorbic to thnh phc molybden mu xanh. Sau em o hp thu quang ti bc sng 880 nm xc nh phospho tng, mu c v c ha chuyn cc dng phospho v orthophosphate, sau xc nh hm lng orthophosphat nh trn. Lu : Khi p dng nh lut Lambert-Beer th phi lu n khong tuyn tnh ca th c th o quang ca dung dch, t xc nh c nng ca cht cn phn tch. Nu A nm ngoi khong tuyn tnh th ty vo ngun gc ca mu m ta tin hnh pha long hoc lm giu mu A nm trong khongtuyn tnh ca nh lut.

III.

CHUN B

3.1 Pha hn hp thuc th Mu hn hp thuc th do 2 nhm pha chung Hn hp gm: 100 mL dd H2SO4 5N 10 mL dd Potassium antimonul tartrate 30 mLdd Ammonium molybdate 60 mL dd acid ascorbic
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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Lc sau mi ln thm cc ha cht vo, dd thuc th thu c c mu vng nht v n nh trong 4 gi

3.2 Chun b mu phosphat: Nu mu b c th ta phi lc trc khi x l mu Cho 5 mL mu vo bnh nh mc 50mL, sau cho thm 1 git phenolphthalein, dung dch vn gi nguyn mu ban u, khng b ha nn khng cn cho thm acid Tip theo cho 4 mL hn hp thuc th pha ch trn vo bnh nh mc, lc u, sau dng nc nh mc ln 50 mL, lc ny dung dch xut hin mu xanh dng nht (mu sau khi cho thm hn hp thuc th th c hin tng kt ta xut hin), tip tc lc u, sau dng phu v giy lc lc dung dch trong bnh dnh mc vo erlen 125 mL ri chun b em o quang. Dung dch sau khi lc trong erlen c mu nht hn sao vi dung dch ban u trong bnh nh mc. 3.3 Chun b mu phospho tng Cho 5 mL mu vo erlen 125 mL, thm 1 git ch th phenolphthalein, dung dch khng i mu, sau cho thm 1 git acid H2SO4 m c 98%, cho thm t t khong 30-40 mL nc vo erlen, lc u. Cn khong 0.5g K2S2O8 (cht rn mu trng), sau cho tip vo erlen cha mu, lc u cho cht rn tan hon ton, dung dch vn gi nguyn mu ban u. em dung dch chun b un trn bp in t trong t ht ph mu, chuyn cc dng khc nhau ca phospho c trong mu v dng orthophosphat, un khong 30 pht n khi dung dch trong erlen cn khong 10 mL th ta tt bp in, dung dch nhit phng n khi ngui Cho t t vo dung dch ngui khong 20 mL nc ct, cho thm 1 mL NaOH 6N, vi git ch th phenolphthalein, lc ny dung dch khng mu, sau ta tip tc trung ha dung dch bng cch thm t t dung dch NaOH 6N cho n khi dung dch c mu hng nht th ngng li. Chuyn ton b dung dch mu hng trong erlen 125mL vo bnh nh mc 50 mL, sau dng nc ct trng li erlen ri chyn ton b phn nc trng vo bnh nh mc cha sn dung dch trnh hin tng mt mu. Sau thm 4 mL hn hp thuc th vo bnh nh mc, lc u, lc ny dung dch chuyn sang mu xanh dng nht, ri dng nc ct nh mc thnh 50 mL ri tin hnh o mu cng dy chun.

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

3.4. Dng dy chun Dy chun gm 7 bnh nh mc 50 mL vi hm lng phospho t 0.1 1.0 mg/L Thc hin pha dy chun nh sau: Bnh Dd phosphat 10 g/mL (mL) Hn hp thuc th (mL) 1 0.0 4 2 0.5 4 3 1.0 4 4 2.0 4 5 3.0 4 6 4.0 4 7 5.0 4

Sau dng nc nh mc thnh 50 mL v dy chun ny c mu xanh dng t nht n m, ring bnh s 1 th khng c mu th l mu trng. Mu xanh ca bnh phosphat nht hn mu xanh ca bnh phospho tng.

IV.

KT QU THU C

Sau khi thc hin xong cc bc chun b mu v dy chun th ta tin hnh o quang, nn o dy chun trc 30 pht trnh hin tng cc phc mu b phn hy gy ra sai s Kt qu bng s liu o c: Bnh Nng hp thu A 1 0 0 2 0.1 0.07 3 0.2 4 0.4 5 0.6 6 0.8 7 1 phosphat 0.44 P tng 0.68 0.413

0.128 0.299 0.421 0.477 0.541 0.275

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Dy chun hp thu A theo hm lng P (g/50mL)

0.7 0.6

0.5
0.4

hp thu A

0.3 0.2 0.1 0 0 0.2 0.4 0.6 0.8 1 1.2

y = 0.5615x + 0.0279

Nng (g/mL)

Phng trnh hi quy tuyn tnh gia hm lng P (g/50mL) v hp thu A A = 0,5615. C + 0,0297 Th kt qu hp thu A o c vo phng trnh trn s thu c kt qu sau: Nng phosphate l = 0.44 (g/mL)

Nng phospho tng l Nhn xt:

0.68 (g/mL)

Kt qu o c nm trong khong tuyn tnh Nng phospho tng ln hn nng phosphat

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bi 5 XC NH HM LNG CN L LNG
I. CCH TIN HNH I.1. Cn khi lng ca giy lc: t giy lc vo phu lc, ra sch bng nc ct. Bt my ht chn khng lm kh giy lc. Ly giy lc ra bng kp nha v vo a thy tinh. Mang a thy tinh i sy kh trong t sy bng nhit 105oC khong 2h. Sau khi sy, ly a thy tinh ra v em i lm ngui trong bnh ht m trong khong 20 pht, ri ly giy lc ht m ra i cn. Tip tc em giy lc i ht m trong vng 20 pht ri li tin hnh cn. Nu chnh lch mi ln cn l 5x10 -4g th ta chp nhn kt qu. Ta ghi ra kt qu khi lng tht ca giy lc. Trong trng hp, kt qu chnh lch ln hn 5x10-4g th tip tc ht m v cn tha mn chnh lch trn. I.2. Cn khi lng giy lc c cn l lng: ong dung dch cn l lng (mu chun b sn) bng ng ong n 150 ml. t giy lc trn vo phu lc. Trng qua mt ln bng nc ct. Cho ton b dung dch trn i qua giy lc. Sau dng nc ct trng li ng ong dung dch cn l lng ( khng b st lng cn cn bm trn thnh ng ong) ri tip tc cho qua giy lc (Thc hin trng ng ong 2 ln). Bt my ht chn khng n khi no mt giy lc kh li. Sau khi ht chn khng , b giy lc cho cn i qua ln a thy tinh v em i sy kh trong vng 2h nhit 105oC trong t sy. Sau , em giy lc i lm ngui trong bnh ht m trong vng 20 pht. Tin hnh cn i vi giy lc va ht m. Ghi li kt qu. Sau li tip tc mang i ht m trong 20 pht. Ri li tin hnh cn. Ghi li kt qu. Nu kt qu chnh lch khng qu 5x10 -4g th kt qu chp nhn c. Trong trng hp, kt qu chnh lch ln hn 5x10-4g th tip tc ht m v cn tha mn chnh lch trn. II. KT QU THU C Ln o Khi lng giy lc (g) Khi lng giy lc c cn(g) 1 0.087 0.107 2 0.087 0.107 K hiu B A

Hm lng cn l lng c tnh theo cng thc: SS (mg/L) =

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= 0,133
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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

Bi 6 HNG DN XC NH COLIFORM
NGUYN TC. Coliform c nh lng bng phng php MPN (Most Probale number) cn gi l phng php pha long ti hn, hay l phng php chun . Phng php ny cho php nh gi s lng vi sinh vt theo xc xut ln nht c th c trong mt n v th tch mu vi chnh xc tng i cao. Phng php MPN trong bi ny c thc hin trn mu 3 nng pha long khc nhau. CCH TIN HNH. 2.1. chun b nt bng bng bng khng thm: 2.2 chun b dung dch mi trng: Dung dch Lauryl tryptose broth l mi trng pht trin ca vi khun, dng pht hin chng vi khun khc nhau. Cch pha: Ta cn pha mi trng cho 9 ng nghim, mi ng cha 9mL dd mi trng, vy ta cn khong 120mL dd lauryl tryptose broth Lauryl tryptose broth: cn khong 2.4 g ri cho vo 120 mL nc ct, khuy u, sau chuyn vo 9 ng nghim c sn ng durham, y li bng nt bng Hp kh trng 9 ng nghim 121 C trong vng 15 pht. BGBL: l mi trng sng c trng ca Coliform, nu l ng (+) th trong cc loi vi khun pht hin c Coliform. Cn pha mi trng BGBL khong 120 mL cho 9 ng nghim Cn 4.8g BGBL cho vo khong 120 mL nc ct, khuy u cho mi trng vo ng nghim c cha durham, y kn bng nt bng v hp kh trng 121 C trong vng 15 pht. Dung dch mui sinh l: dng pha long mu Mui NaCl 8.5 g/L, ta cn pha khong 250 mL dung dch mui dng chung cho 2 nhm Cn khong 2.2 g NaCl cho vo 250 mL nc ct, khuy u, gi kn bng giy bc sau em hp kh trng cng lauryl trytose broth, BGBL trong ng nghim v cc u ca micropipet. 2.3 Pha long mu. Mu c pha long tun t thnh cc nng thp phn 1/10; 1/100; 1/1000 . Cch pha l ly 1ml mu hoc l dung dch c pha long trc thm vo 9ml NaCl trong mt ng nghim sau khi lc k s c nng pha long 1/10.
Thc hnh ha ng dng mi trng 2010

I.

II.

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Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

2.4. Tin hnh Sau khi pha long mu 3 nng nh trn th by gi cho mi nng pha long s dng 3 ng nghim - 3 nng s dng 9 ng nghim. Ly ly 1 mL mu c pha long cho vo ng nghim c cha mi trng lauryl tryptose broth, sau 37 C trong 48h. Sau 48h mnh ghi nhn s ng c sinh hi. Sau dng que cy, vng cy chuyn dch t nhng ng sinh hi sang cc ng cha BGBL v tip tc 37 C trong vng 48h. Sau 48h ghi nhn s ng c kt qu (+) ng vi mi pha long. III. KT QU THU C S lng ng c kt qu dng tnh trong mi trng lauryl tryptose sau 48h l 7 c th nh sau S ml mu s dng 10 3 1 3 0.1 1

S lng ng c kt qu dng tnh trong mi trng BGBL sau 48h l 6 ng c th nh sau S ml mu s dng 10 3 1 3 0.1 0 S MPN/100 ml 240

Ch :

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Thc hnh ha ng dng mi trng 2010

Nhm 27: Kiu Th Phng Loan - 0717053 _ V Th Hong Yn - 0717139

a s cc bng MPN cung cp s liu dng s MPN trong 100ml dung dch c s dng phn tch cc liu lng l 10ml, 1ml, 0.1 ml. Trn thc t phn tch, ngi ta thng dng 1ml cho mu pha long 10-1, 10-2, 10-3. Lng mu vi cc pha long ny tng ng vi 1/100 lng mu s dng theo bng MPN/100ml tnh theo lng mu l 10ml, 1ml, 0.1 ml nu trn. Vy s liu ca bng MPN/100ml tnh theo lng mu l 10ml, 1ml, 0.1 ml tng ng vi MPN/ml mu cha pha long khi 1ml ca cc dung dch c pha long 10-1, 10-2, 10-3 c dng phn tch. Trng hp mt vi sinh vt qu cao, cn phi s dng lot nng pha long tip theo cn phi nhn tr s tra bng MPN nu trn vi h s pha long. Trong bng l s dng kt qu cho 10 ml mu, m mu nhm lm ch c 1ml vy kt qu cui cng nhn thm vi 10 Kt qu l 240*10 = 2400 MPN

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Thc hnh ha ng dng mi trng 2010