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Phn I Thuc tim & thuc nh mt

THUC NH MT CHLORAMPHENICOL
1. YU CU
Thuc nh mt Chloramphenicol 0,4%:
Nng ca hot cht: 0,4%.
Lng thuc nh mt cn iu ch: 200 ml.
Yu cu pH: hi acid. Ta chn pH = 6,8.
2. TNH TON
Lng hot cht cn s dng: 200 0,4% = 0,80 g.
H m: H m s dng t c pH= 6,8: H m Acid boric borax [1]
Dung dch Borax 0,05M
Dung dch Acid boric 0,2M
pH
Na2B2O7.10H2O P.t.l: 381,83
H3BO3 P.t.l: 61,84
3 ml
97 ml
6,8
pha 200 ml dung dch c pH = 6,8 chng ta cn:
381,83 0,05 3
61,84 0,2 97
2 0,12 g Borax v
2 2,40 g Acid boric.
1000
1000
ng trng ha:[2]
Cng thc LUMIRE CHEVROTIER:
Trong :
0,52: h bng im ca dung dch NaCl 0,9%.
0,52 t1 t
x=
1 : h bng im ca dung dch nhc trng.
t 2
t 2 : h bng im ca dung dch 1% ca cht dng ng
trng ha. y chng ta dng NaCl th t 2 = 0,58 .
h bng im ca mt s cht nng 1%:
T f1% (oC)
Dung dch
Chloramphenicol
- 0,06
Acid boric
- 0,288
Borax
- 0,241
(Tham kho bng 6, trang 53, British Pharmaceutical Codex 1973)
h bng im ca dung dch khi cha thm NaCl (Chloramphenicol, acid boric,
borax)
0.80 100
0,12 100
2,4 100
Tf =
()0,06 +
()0,241 +
()0,288 = ()0,38( o C)
200
200
200
Lng NaCl cn thm vo c 200 ml dung dch ng trng l:
0,52 0,38 200
m NaCl =

0,48 g.
0,58
100

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Phn I Thuc tim & thuc nh mt

Cht bo qun: [3] Cht bo qun c s dng l Nipagin. Ta dng Nipagin M vi t

200 0,05%
= 0,50 ml. ( thun tin
l 0,05% nn lng Nipagin M 20% cn dng l:
20%
hn trong thc tp, ta s dng dung dch m Nipagin 20%).
3. CNG THC HON CHNH
Thnh phn
Chloramphenicol
Borax
Acid boric khan
Natri chloride
Dung dch Nipagin M 20%
Nc ct pha tim

Lng dng
0,80 g
0,12 g
2,40 g
0,48 g
0,50 ml
v 200 ml

Thnh phm
Chloramphenicol
0,4%
(pH 6,8)

Ti liu tham kho:

[1] [2] [3]: NXB Y hc, Bo ch v sinh dc hc - Tp 1, 2011, Thuc nh mt Collyria,
trang 201, 203, 197.

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Phn I Thuc tim & thuc nh mt

THUC NH MT KM SULFATE
Bi tp cho pH la chn theo dc in Anh. Tuy nhin theo tm hiu thng tin,Dc in
Anh qui nh dung dch km sulfate dng cho thuc nh mt c pH=4,4 - 5,6 [1] ,hon ton
ging Dc in Vit Nam IV. Do nhm xut la chn cng thc iu ch thuc nh
mt km sulfate c pH = 5,9 nm trong vng pH = 5,8 - 6,2 c qui nh theo Dc in
M.[2]
I. KM SULFATE 0,5%:
1. Tnh ton pH
Ta c pH = 5,9 [ H + ] = 10-5,9
Ta c hng s phn ly ca acid boric pKa = 9,2.
+
H3BO3 H 2 BO 3 + H +
x
10-5,9
10-5,9 10-5,9
-5,9
(x-10 )
10-5,9 10-5,9
2
10 5,9

= 10 9, 2 x = 2,5.10-3
x 10 5,9
nH3BO3 = 2,5.10-3 0,2 = 5.10-4(mol)
mH3BO3 = 5.10-4 61,8 = 0,03(g).
2. Tnh ton ng trng:
Cng thc LUMIRE CHEVROTIER:
Trong :
0,52: h bng im ca dung dch NaCl 0,9%.
0,52 t1 t
x=
1 : h bng im ca dung dch nhc trng.
t 2
t 2 : h bng im ca dung dch 1% ca cht dng ng
trng ha. y chng ta dng NaCl th t 2 = 0,58 .

Bit: t ca km sulfat 1% l - 0,083 0C

t ca km sulfat 0,5% l - 0,041 oC
t ca acid boric 1% l - 0,288oC
Thay cc gi tr vo cng thc LUMIRE CHEVROTIER, lng NaCl cn thm vo
c 100 ml dung dch ng trng l:
0,03

0,52 0,083 0,5 +

100 0,288
200

= 0,82(g )
x=
0,58
Lng NaCl cn thm vo c 100 ml dung dch ng trng= 0,82 2=1,62(g)
3. Tnh ton lng cht bo qun:
Nipagin M dng theo t l l 0,05%
pha 200ml thuc nh mt trn cn lng Nipagin M 20% l:

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(0,05 x 200)/20 = 0,5ml

4. Cng thc hon chnh:
Km sulfat:
1,00 g
Acid boric:
0,03 g
NaCl
1,62 g
Nipagin M 20%:
0,5 ml
Nc ct pha tim:
v 200 ml
II. KM SULFATE 0.25%
1. Tnh ton pH:
Ta c pH = 5,9 [H+] = 10-5,9
H3BO3 H2BO3- + H+
x
10-5,9
10-5,9
10-5,9
-5,9
-5,9
(x-10 )
10
10-5,9
Ta c hng s phn ly ca acid boric pKa=9,2
2
10 5,9
= 10 9, 2 x=2,5 10-3
5,9
x 10
nH3BO3 = 2,5 10-3 0,2 = 5.10-4(mol).
mH3BO3 = 5 10-4 61,8 = 0,03(g).
2. Tnh ton ng trng:
Cng thc LUMIRE CHEVROTIER:
Trong :
0,52: h bng im ca dung dch NaCl 0,9%.
0,52 t1 t
x=
1 : h bng im ca dung dch nhc trng.
t 2
t 2 : h bng im ca dung dch 1% ca cht dng ng
trng ha. y chng ta dng NaCl th t 2 = 0,58 .

Bit:

t ca km sulfat 1% l -0,083 oC
t ca km sulfat 0,5% l -0,041 oC
t ca acid boric 1% l -0,288oC

(Tham kho bng 6, trang 53, British Pharmaceutical Codex 1973)

Thay cc gi tr vo cng thc LUMIRE CHEVROTIER, lng NaCl cn thm vo c
100 ml dung dch ng trng l:
0,03

0,52 0,083 0,25 +

100 0,288
200

= 0,85(g)
x=
0,58
Lng NaCl cn thm vo c 100 ml dung dch ng trng = 0,85 2=1,7(g)
3. Tnh ton lng cht bo qun:

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Phn I Thuc tim & thuc nh mt

Nipagin M dng theo t l l 0,05%

pha 200ml thuc nh mt trn cn lng Nipagin M 20% l:
(0,05 x 200)/20 = 0,5ml
4. Cng thc hon chnh:
Km sulfat:
0,5 g
Acid boric:
0,03 g
NaCl
1,7 g
Nipagin M 20%:
0,5 ml
Nc ct pha tim:
v 200 ml
Ti liu tham kho:
[1]: Zinc Sulphate Heptahydrate,Medicinal and Pharmaceutical Substances,Volume I&II,
British Pharmacopoeia 2009, The Stationery Office, tr 6454.
[2]: Zinc Sulfate Ophthalmic Solution, Ophthalmic and Dermatologicals,USP31-NF26, tr
3551.

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