VIN NN ACETAZOLAMID Tabellae Acetazolamidi L vin nn cha acetazolamid Ch phm phi p ng cc yu cu trong chuyn lun "Thuc vin nn"

(Ph lc 1.20) v cc yu cu sau y : Hm lng acetazolamid, C4H6N4O3S2, t 95,0 - 105,0% so vi hm lng ghi trn nhn. Tnh cht Vin mu trng. nh tnh A. Cn mt lng bt vin tng ng vi khong 0,5 g acetazolamid, thm 2 ml dung dch natri hydroxyd 1 M (TT). Lc k, lc, trung tnh dch lc bng acid acetic bng (TT), lc ly ta, sy ta thu c 105oC n cn kh. Ph hng ngoi (Ph lc 4.2) ca cn thu c phi ph hp vi ph i chiu ca acetazolamid. B. Nghin mt lng bt vin tng ng vi khong 0,5 g acetazolamid vi hn hp gm 5 ml nc v 1 ml dung dch natri hydroxyd 1 M (TT), cho hn hp thu c vo ng nghim, thm 0,2 g km bt (TT) v 0,5 ml dung dch acid hydrocloric (TT), lp tc y mnh giy c tm dung dch ch acetat (TT) ln trn ming ng nghim, trn giy s xut hin mu en nu. C. Cn mt lng bt vin tng ng vi khong 25 mg acetazolamid thm 5 ml nc, 0,15 ml dung dch natri hydroxyd 1 M (TT), lc, thm 0,1 ml dung dch ng sulphat 10 % (TT), s xut hin mu lam nh lc hoc c ta. Tp cht lin quan Phng php sc k lp mng (Ph lc 5.4) Bn mng: Silicagel GF254 Dung mi khai trin: Propan-2-ol - ethyl acetat - dung dch ammoniac 13,5 M (50 : 30 : 20) Dung dch (1): Cn chnh xc mt lng bt vin tng ng vi khong 50 mg acetazolamid, thm 10 ml hn hp dung mi ng th tch ethanol 96% v ethyl acetat, lc k trong 20 pht, lc. Dung dch (2): Ht chnh xc 1 ml dung dch (1), pha long vi hn hp dung mi trn va 100 ml. Cch tin hnh: Chm ring bit ln bn mng 20 l mi dung dch trn. Trin khai sc k n khi dung mi i c khong 15 cm. Ly bn sc k ra v kh ngoi khng kh. Quan st di nh sng t ngoi bc sng 254 nm. Trn sc k , bt k vt ph no ca dung dch (1) cng khng c c mu m hn mu ca vt chnh ca dung dch (2) (1%). nh lng Phng php chun o in th (Ph lc 10.2) Cn 20 vin, tnh khi lng trung bnh vin, nghin thnh bt mn. Cn chnh xc mt lng bt vin tng ng khong 0,4 g acetazolamid, thm 90 ml dung dch dimethylformamid (TT). Chun bng dung dch tetrabutylammonium hydroxyd 0,1 M (C) . Xc nh im kt thc bng phng php chun o in th. 1 ml dung dch tetrabutylammonium hydroxyd 0,1 M (C) tng ng vi 22,22 mg C4H6N4O3S2 Bo qun ng trong bao b kn, ni kh mt, trnh nh sng. Loi thuc Thuc chng glcm. Hm lng thng dng 125 mg, 250 mg.