Bi 1 : PHA DUNG DCH CHUN IOD 0,1N 1.

Nguyn tc : Dung dch chun iod 0,1N c pha t ha cht iod v Kali iodid. Xc nh h s hiu chnh K bng dung dch chun Na2S2O3 (k = 1,000): I2 + 2Na2S2O3 2 NaI + Na2S2O4 H s hiu chnh K ca dung dch Iod 0,1N : K = V0K0/V K : h s hiu chnh phi tm. V0 : th tch dung dch Na2S2O3 tiu th K0 : H s hiu chnh ca dung dch Na2S2O3 V : th tch dung dch Iod 0,1N dng. 2. Ha cht : - Dung dch chun Na2S2O3 0,1N - Iod - Kali iodid - Dung dch h tinh bt 1% 3. Dng c : - 1 bnh nh mc 250ml - 3 bnh nn nt mi 100ml - 1 cc c m 250ml - 2 cc c m 100ml - Phu thy tinh - Burette v gi - 1 pipet bu 25ml - 3 pipet vch 5ml, 10ml - a thy tinh - 1 ng ong 50ml 4. Cch tin hnh : Cn 3,25g Iod tinh th, sau cn tip 5,00g Kali Iodid trong mt cc c m 250ml. Thm 30ml nc v khuy trong t ht cho n khi tan hon ton. Thm khong 100ml nc v lc qua phu c bng ht nc vo bnh nh mc 250ml. Trng cc bng 50ml nc ct v lc tip vo bnh nh mc ny. Thm nc n vch, y nt, lc u. Ly ng 25,0ml dung dch Iod va pha, cho vo bnh nn c nt mi, thm 30ml nc v chun bng dung dch Na2S2O3 0,1N cho n khi c mu vng nht, thm 2ml dung dch h tinh bt v chun tip cho n khi mt mu. Nu k > 1,000 ta tnh c lng nc cn thm vo theo cng thc : (k1)V, vi V l th tch ca dung dch chun cn phi hiu chnh c k=1,000. Sau khi thm nc hiu chnh, chun li nh trn xc nh li h s K.
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Bi 2 : KIM NGHIM NGUYN LIU NATRI HYDROCARBONAT

1. Tnh cht : Bt kt tinh trng, khi un nng trng thi kh hay trong dng dch, ch phm chuyn dn sang Natri Carbonat. Tan trong nc, thc t khng tan trong ethanol 96%. 2. nh tnh : a. Thm vo 5ml dung dch S (dung dch 5%) 0,1ml dung dch phenolphtalein (CT), mu hng xut hin, un nng c bt kh bay ln v dung dch chuyn sang mu . b. Ly 2ml dung dch S cho vo ng nghim, thm 2ml dung dch Magnesi sulfat 5%, dung dch vn trong, un si s cho ta trng. c. Ly 2ml dung dch S, acid ha bng acid acetic long, thm 1ml thuc th Streng (Magnesi uranyl acetat), c thnh ng nghim bng a thy tinh (nu cn) s xut hin ta vng mn. 3. trong v mu sc dung dch : Dung dch S : Ha tan 5,0g Natri hydrocarbonat trong 90ml nc khng c CO2 v nh mc thnh 100ml. Dung dch S phi trong v khng mu. 4. Carbonat : pH ca dung dch S mi khng c qu 8,6. 5. Clorid : Khng c qu 0,015%. Thm vo 7ml dung dch S 2ml acid nitric long v 6ml nc (thnh 15ml) v tin hnh th. 6. Sulfat : Khng c qu 0,015%. Thm acid hydroclorid (TT) v dung dch cha 1,0g ch phm trong 10ml nc trung tnh (khong 1ml), pha long thnh 15ml v tin hnh th. * Pha dung dch sulfat mu 10ppm : Ht 1ml dung dch sulfat mu 1000ppm cho vo bnh nh mc 100ml v thm nc n vch. Trn u, ta c dung dch sulfat mu 10ppm. - ng th : thm 1ml dung dch BaCl 2 25% vo 1,5ml dung dch sulfat mu 10ppm, lc v yn 1 pht, thm 15ml dung dch ch phm th, yn 5 pht. - ng mu : thm 1ml dung dch BaCl 2 25% vo 1,5ml dung dch sulfat mu 10ppm, lc v yn 1 pht, thm 15ml dung dch sulfat mu 10ppm, lc u, yn 5 pht. So snh c ca ng th v ng mu.

7. Amoni : Khng c qu 20ppm. Ly 10ml dung dch S, pha long thnh 15ml, ri tin hnh th. - ng th : thm vo ng nghim cha 15ml dung dch trn 0,3ml thuc th Nessler, lc u, yn 5 pht. - ng mu : ly chnh xc 10ml dung dch Amoni mu 1ppm, pha long thnh 15ml, thm 0,3ml thuc th Nessler, lc u, yn 5 pht. So snh mu to thnh trong 2 ng th v ng mu. * Pha dung dch Amoni mu 1ppm : Ht 1ml dung dch Amoni mu 100ppm cho vo bnh nh mc 100ml, thm nc n vch, lc u ta c dung dch Amoni mu 1ppm. 8. Calci : Khng c qu 0,01%. Thm HCl (TT) vo hn dch cha 1g ch phm trong 10ml nc cho n khi trung tnh v pha long vi nc thnh 15ml v tin hnh th. - ng th : thm 1ml amoni oxalat 4% vo 0,2ml dung dch Calci mu 100ppm trong ethanol 96%, sau 1 pht, thm vo hn hp gm 1ml CH 3COOH 2M v 10ml Calci mu 10ppm, thm tip 5ml nc. Lc u, yn 15 pht. * Dung dch Calci mu 100ppm trong ethanol 96% : ly 1ml dung dch Calci mu 100ppm, cho vo 9ml ethanol 96%, nh mc thnh 10ml. * Dung dch Calci mu 10ppm : ly 1ml dung dch Calci mu 1000ppm cho vo bnh nh mc 100ml, thm nc n vch, trn u. 9. St : Khng c qu 20ppm. Ha tan 0,5g ch phm trong 5ml HCl long, pha long thnh 10ml vi nc v tin hnh th. 10. Kim loi nng : Khng c qu 10ppm. Ha tan 2,0g ch phm trong hn hp gm 2ml HCl v 18ml nc. Ly 12ml dung dch ny v tin hnh th. 11. nh lng : Natri hydrocarbonat phi cha t 99,0 n 101,0% NaHCO3 . Ha tan 1,500g ch phm trong 50ml nc khng c CO 2 . Chun bng dung dch acid hydroclorid 1M, dng 0,2ml da cam methyl lm ch th. 1ml HCl 0,1M tng ng vi 84,0mg NaHCO3 . Cch tnh : n(ml)* K * 84,0(mg) * 100 C% = P vi : K l h s hiu chnh P l khi lng bt cn nh lng (mg).

Bi 3 : PYRIDOXIN HYDROCLORID
1. Tnh cht : Bt kt tinh trng hay gn nh trng, chy 250C km theo phn hy. D tan trong nc, kh tan trong ethanol 96%, thc t khng tan trong cloroform v ether. 2. nh tnh : a. Ph hp th nh sng t ngoi ca dung dch 0,001% trong acid hydroclorid 0,1M c 1 cc i khong 288-290nm bc sng cc i ny t 420-445. b. Ha tan 0,1g ch phm trong 10ml nc, ha bng 0,5ml acid nitric 2M, thm 0,4ml dung dch bc nitrat 2%, lc u to thnh ta trng ln nhn. 3. trong v mu sc dung dch : Dung dch S : Ha tan 2,50g ch phm trong nc khng c carbon dioxyd c 50ml, dung dch S phi trong v khng c c mu m hn mu mu V7. - Ha tan 46g st III clorid trong acid hydroclorid (25ml HCl + 975ml nc) 1000ml. - Ha tan 60g CoCl2 (Cobalt Clorid) trong dung mi A (25ml HCl + 975ml nc) 1000ml. Ly 24ml mu vng + 6ml mu + 70ml HCl 1% Sau ly 2,5ml hn hp trn + 97,5ml HCl 1% dung dch V7. 4. pH : ca dung dch S t 2,4 n 3,0 5. Kim loi nng : Khng c qu 20ppm Ly 12ml dung dch S th theo phng php 1 Dng dung dch ch mu 1ppm chun b mu i chiu 6. Mt khi lng do lm kh : Khng c qu 0,05% (1g, 100 - 105C) 7. nh lng : Ha tan 0,15g ch phm trong hn hp gm 5ml acid acetic khan (TT) v 6ml dung dch thy ngn II acetat (TT). Chun bng dung dch acid perclorid 0,1M, dng 0,05ml dung dch tm tinh th (CT) lm ch th cho n khi dung dch c mu xanh lc. 1ml dung dch acid perclorid 0,1M tng ng vi 20,56mg C8H11NO3.HCl Pyridoxin hydroclorid phi cha t 99,0- 101,0% C8H11NO3.HCl tnh theo ch phm lm kh.

Bi 4 : KIM NGHIM NC OXY GI LONG (Chai 25,0ml hoc 30,0ml)

1. Nguyn tc : Nc oxi gi long c xc nh nng bng phn ng oxy ha kh vi dung dch chun Kali permanganat trong mi trng acid sulfuric. Hm lng H2O2 c trong nc H2O2 long c xc nh bng phn ng vi KMnO4 nh sau: 2KMnO4 + 5H2O2 + 3H2SO4 = K2SO4 + 2MnSO4 + 8H2O + 5/2O2 2. Kim nghim : 2.1. Tnh cht : Chai phi kn, nhn ng quy nh, ng dung dch trong khng mu. 2.2. ng u th tch : Dng ng ong kh sch c dung tch 25,0ml hoc 50ml xc nh th tch ca 5 n v ng gi bt k. C 5 n v u phi nm trong gii hn cho php. - L 25,0ml : phi t t 25,0 n 27,5ml - L 30ml : phi t t 30,0 n 33ml. 2.3. nh tnh : - Lc 0,5ml ch phm vi 2ml dung dch H2SO4 10%, 2ml ether ethylic v 0,5ml dung dch Kali dicromat 5%, lp ether c mu xanh m. - Lc 2ml ch phm vi 0,2ml dung dch H 2SO4 10%, 0,25ml KMnO4 0,1M. Sau vi giy, dung dch mt mu hng hay c mu hng rt nht. 2.4. Acid : Pha long 10ml ch phm vi 20ml nc un si ngui, thm 0,25ml dung dch methyl, lng NaOH 0,1N thm vo lm chuyn mu dung dch khng c t hn 0,05ml v khng nhiu hn 1,0ml. 2.5. nh lng : Nc oxi gi long phi cha t 2,5 n 3,5g H2O2 trong 100ml. - Tin hnh : Ly chnh xc 2,0ml dung dch ch phm vo bnh nn nt mi cha sn 20ml nc, thm 20ml dung dch H 2SO4 10% v chun bng dung dch KMnO4 0,1N. 1ml dung dch KMnO4 0,1N tng ng vi 0,001701g H2O2 . Lng oxi gi long c trong 100ml dung dch c tnh theo cng thc : n(ml)*K*0,001701*100(ml) Cg/100ml = 2(ml) Vi n l s ml dung dch KMnO4 0,1N chun .

Bi 5 : KIM NGHIM VIN BAO PHIM VITAMIN C 500MG

1. Nguyn tc : Vitamin C (acid ascorbic) c chun bng php o iod theo phn ng : C6H8O6 + I2 C6H6O6 + 2HI cui nh lng, 1 lng tha Iod s tc dng vi h tinh bt v cho mu xanh en. 2. Ha cht, dng c v thuc th : a. Ha cht v thuc th : - Dung dch chun iod 0,1N - Dung dch NaOH 2M - Dung dch Natri nitro pusiat 5% - Dung dch Diclorophenol indophenol - H tinh bt 1% - Dung dch HCl 25% - Dung dch AgNO 3 b. Dng c : - 3 bnh nn 100ml - 3 cc c m 100ml, 250ml - ng nghim - ci chy - Burette v gi - ng ong 10ml - Pipet 10ml, 5ml 3. Cch tin hnh : a. Tnh cht : Vin phi lng nhn, mu u, cnh v thn phi lnh ln. b. ng u khi lng : Xc nh trn 20 vin, khi lng trung bnh vin : m 5%. c. tan r : Khng c qu 30 pht. d. nh tnh : - Ly mt lng ch phm c nghin mn, tng ng vi khong 0,1g acid ascorbic, lc vi 10ml nc. - Dch lc lm giy qu xanh. - 3ml dch lc + 0,5ml AgNO3 2% cho ta en. e. nh lng : Cn 20 vin, tnh khi lng trung bnh M, nghin bt mn v cn mt lng bt vin tng ng vi 200mg Vitamin C, cho vo bnh nn nt mi
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dung tch 100ml, thm 20ml nc ha tan. Chun bng dung dch Iod 0,1N cho n khi xut hin mu xanh bn vng trong 30 giy ( ch th H tinh bt 1% ) 1ml dung dch Iod 0,1N tng ng vi 8,806mg Vitamin C Tnh kt qu : N(ml)* K* 8,806(mg)*M(mg) C% = P(mg)*500(mg) Vin bao phim Vitamin C phi cha t 95,0 n 110% hm lng ghi trn nhn.

Bi 6 : KIM NGHIM THUC GI SORBITOL

1. Tnh cht : Thuc bt mu trng, kh ti, khng mi, v ngt mt. Th bng cm quang. 2. ng u khi lng : 5,0g 5% Cn bng cn k thut 5 n v ng gi bt k, tt c cc gi phi nm trong gii hn cho php. 3. Gim khi lng do sy kh : Khng c qu 2%. Cn khong 1g ch phm v sy nhit 70OC trong 3 gi. Khi lng mt i khng c qu 2%. 4. nh tnh : Ha tan 0,1g ch phm trong 2ml nc, thm 1 git NaOH 1N v 5 git KMnO4 0,1M, em un ti si s mt mu hng ca thuc tm, thm 2ml thuc th Fehling, un si s c ta . 5. nh lng : Hm lng Sorbitol phi nm trong khong 4,750 n 5,250g tnh theo khi lng trung bnh gi. Cn chnh xc khong 0,100g ch phm vo bnh nh mc 100ml, thm khong 70ml nc, lc ha tan ri thm nc ti vch. Ly chnh xc 10ml dung dch cho vo bnh nn nt mi, thm chnh xc 25ml dung dch Kali periodat 0,3% v 1ml acid sulfuric m c, un cch thy khong 15 pht, sau khi lm ngui, thm 2ml dung dch Kali iodid 30%. yn 5 pht. Sau , chun Iod phng thch bng dung dch Na2S2O3 0,1N, ch th h tinh bt. Thc hin song song mu trng trong cng iu kin. 1ml dung dch Na2S2O3 0,1N tng ng vi 0,00182g Sorbitol. Hm lng Sorbitol trong mt gi c tnh theo cng thc : (N-n)*0,00182*100(ml)*m*100 C% = 10(ml)*p Trong : N : s ml Na2S2O3 0,1N chun mu trng n : s ml Na2S2O3 0,1N chun mu th p : khi lng bt cn nh lng m : khi lng trung bnh gi.

Bi 7 : KIM NGHIM THUC TIM VITAMIN C

1. Tnh cht : ng hn kn, nhn ng quy ch ng dung dch trong khng mu hay mu vng nht. 2. ng u th tch : Th tch mi ng phi t V n V + 15% V l th tch ghi trn nhn Xc nh trn 5 ng thuc bt k, dng bm tim kh, sch xc nh th tch 3. pH : 5,0 - 7,0 Cho khong 20 - 30ml ch phm vo cc c m dung tch khong 5ml kh sch v o bng my o pH. 4. Mu sc : Nu cn pha long mt th tch ch phm vi nc c 1 dung dch c nng 50mg Acid ascorbic/ml. o hp th ca dung dch ny bc sng 420nm, cc o 1cm, mu trng l nc ct. hp th khng c ln hn 0,06. 5. nh tnh : a. Ly 1 lng ch phm c cha khong 50mg Acid ascorbic, thm 0,2ml HNO3 2M v 0,2ml dung dch AgNO3 2% : Xut hin ta mu xm en. b. Ly 1 lng ch phm c cha khong 50mg Acid ascorbic, thm 0,1ml 2,6-Diclophenol indophenol mu xanh ca thuc th b mt. 6. Acid oxalic : Khng c qu 0,3%. - Dung dch th : Ly chnh xc mt lng ch phm cha 250ml Acid ascorbic, nu cn pha long va 5ml trung ha bng dung dch NaOH 10%, thm 1ml dung dch CH3COOH 2M v 0,5ml dung dch CaCl2 10%. - Dung dch i chiu : Ha tan 700mg Acid oxalic trong 500ml nc. Ly 5ml dung dch ny, thm 1ml dung dch CH 3COOH 2M v 0,5ml CaCl2 10%. yn trong 1 gi. Dung dch th khng c c hn dung dch i chiu. 7. nh lng : Ch phm thuc tim Vitamin C phi cha Acid ascorbic, thm 0,25ml dung dch Formaldehyd 1%, 4ml dung dch HCl 2%, 0,5ml dung dch KI 10% v 2ml dung dch h tinh bt. nh lng bng dung dch Kali iodat 0,1N cho n khi xut hin mu xanh lam nht bn vng.

1ml dung dch Kali iodat 0,1N tng ng 8,806mg C6H8O6 Cch tnh kt qu: n(ml) * K * 8,806mg * 100 C% = V(ml) * a V : Th tch ch phm ly nh lng A : Nng ch phm (mg/ml).

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Bi 8 : KIM NGHIM NGUYN LIU MAGNESI SULFAT

1. Nguyn tc : Magnesi sulfat c nh lng bng phng php Complexon, cc ion kim loi nh ch, calci, bismuth, magnesi s to phc vi dung dch EDTA (Ethylen diamin tetra acetic acid), do c ch Chelat (kim ha). 2. Kim nghim : a. Tnh cht : Bt kt tinh trng hay tinh th khng mu, bng. D tan trong nc, rt d tan trong nc si, thc t khng tan trong ethanol 960. b. nh tnh : Ha tan 0,5g ch phm trong 10ml nc. Ta c dung dch A. - Magnesi : Ly 2ml dung dch va pha, thm 1 2ml dung dch amoniac 6M ta trng. - Sulfat : Ly 5ml dung dch A, thm 1ml HCl 10%, 1ml dung dch Bari clorid 5% ta trng. c. trong v mu sc dung dch : Dung dch S : Ha tan 0,5g ch phm trong nc va 50ml. Dung dch S phi trong sut v khng mu. d. Gii hn acid kim : Thm 0,05ml phenol (CT) vo 10ml dung dch S, dung dch phi chuyn mu khi thm khng qu 0,2ml HCl 0,01N hay dung dch NaOH 0,01N. e. Clorid : Khng c qu 0,03%. Ly 1,7ml dung dch S pha long thnh 15ml v tin hnh th. - ng th : 15ml dung dch v pha tip thm 1ml HNO 3 2M v 1ml Bc nitrat 2%. Lc u, yn 15 pht v so snh c to thnh trong ng th vi ng chun. - ng chun : + Pha dung dch ion clorid mu 5ppm : Ht 1ml dung dch clorid mu 500ppm, cho vo bnh nh mc 100ml, thm nc n vch, trn u. + Ly 10ml dung dch clorid 5ppm cho vo ng nghim, thm 5ml nc, 1ml acid citric 2M, 1ml bc nitrat 2%, lc u, yn 5 pht ri so snh. f. Kim loi nng : Khng c qu 10ppm. - ng th : Cho vo ng nghim 12ml dung dch S, 2ml dung dch m acetat pH = 3,5 v 1,2ml thuc th Thioacetamid, lc u v so snh mu vi ng mu sau 2 pht.

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- ng mu : Cho vo ng nghim 10ml dung dch ch mu 1ppm v 2ml dung dch S, thm 2ml dung dch m acetat pH = 3,5, ri 1,2ml thuc th Thioacetamid, lc u, yn 2 pht. * Pha dung dch ch mu 1ppm : - Ly 1ml dung dch ch mu 1000ppm, thm 9ml nc, th tch cui l 10ml. - Ly 1ml dung dch mi pha cho vo bnh nh mc 100ml, thm nc n vch. Ta c dung dch ch mu 1ppm. g. St : Khng c qu 20ppm. - ng th : Ly 5ml dung dch S, thm 5ml nc trong ng Nessler, thm 2ml dung dch acid citric 20% v 0,1ml acid mercaptoacetic, lc u, kim ha bng dung dch amoniac 10M v pha long vi nc n 20ml. - ng chun : Cho vo ng Nessler 10ml dung dch st mu 1ppm, 2ml acid citric 20%, thm tip 0,1ml acid mercaptoacetic (TT), kim ha bng dung dch amoniac 10M v pha long vi nc n 20ml. yn 5 pht ri so snh mu to gia ng chun vi ng th. - Pha dung dch st 1ppm : Ly 0,5ml dung dch st mu 200ppm v pha long trong bnh nh mc 100ml bng nc ct ti vch. h. Mt khi lng do lm kh : T 48,0 n 52,0%. Sy 0,500g nguyn liu 110 120OC trong 1gi, sau 400OC. i. nh lng : Magnesi sulfat phi cha t 99,0 n 100,5% MgSO4 tnh theo ch phm lm kh. Ha tan 0,45g ch phm trong 100ml nc, thm 10ml dung dch m Amoniac (pH = 10) v thm khong 50mg hn hp ch th Ericrom en T. Lc u, un nng dung dch n 40OC v chun nhit bng dung dch Trilon B 0,1M cho n khi mu tm chuyn sang mu xanh lam. 1ml dung dch Trilon B 0,1M tng ng vi 12,04mg MgSO4.

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Bi 9 : XC NH M BT PENICILIN V O T TRNG CC DUNG DCH

1. Xc nh m bng phng php sy : m ca bt Penicilin c xc dnh bng phng php sy nhit 100 105OC trong t sy. - Cn xc nh khi lng ca cc cc cn sau khi sy n khi lng khng i nhit 100 105OC v trong bnh ht m c Silicagel trong 10 pht. - Sau , cn mt lng bt cn xc nh m P khong 500mg. Sau , em sy nhit 100 105 OC trong t sy, thi gian sy 1,5 2 gi. Sau ly khi t v trong bnh ht m. Cn v ghi khi lng cc v bt sau khi cn. Gi : Po l khi lng cc cn cha c bt po l khi lng bt Penicilin em sy Pt l khi lng cc cn v bt trc khi sy ( Pt = Po + Pb ) Pt l khi lng cc cn v bt sau khi sy Mt khi lng do lm kh ca bt tnh theo % c tnh theo cng thc : Pt Pt x% = * 100 Pb 2. o t trng cc dung dch : T trng biu kin ca cc dung dch 1, 2, 3 c xc nh bng phng php t trng k. a. iu chnh nhit dung dch v 20OC : t cc ng ong ng dung dch cn o vo cc thy tinh ng nc p vn v dng nhit k xc nh nhit ca dung dch chc chn l dung dch c o t trng 20OC. b. Tin hnh o : Nhng nh nhng t trng k vo dung dch cho ti khi t trng k ngp su vo nc v th tay ra. T trng k khng c chm vo y ng ong hay thnh ng ong. c gi tr t trng ca dung dch trn vch chia ca t trng k.

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Bi 10 : KIM NGHIM VIN NANG AMOXICILIN 500MG

1. Nguyn tc : Trong mi trng kim hay m, cc khng sinh nhm lactam b m vng lactam v mt tc dng khng khun, ta c th gn iod vo cc phn t b m vng trong mi trng acid nh, lng iod tha c xc nh bng dung dch chun Natri thiosulfat. 2. Kim nghim : 2.1. Tnh cht : Nang cng, bt thuc trong nang mu trng hay trng ng, khng mi hay gn nh khng mi. 2.2. ng u khi lng : Khi lng trung bnh bt thuc trong nang : m 7,5%. Cn 20 nang c cha thuc v ghi khi lng Mn, sau cn tng nang mt v tip bt thuc ra, dng tm bng chi sch v nang, ri cn v, hiu s ca nang cha thuc tr i v nang l tr s khi lng bt thuc trong nang, sau khi ln lt xc nh bt thuc ca tng nang mt trong s 20 vin, ta cn 20 v nang rng, ta c Mv. Mn - Mv C% = - m ( khi lng trung bnh bt thuc trong nang ) 20 So snh khi lng bt thuc ca tng nang vi gi tr m 7,5%. Thuc t ng u khi lng nu c khng qu 2 vin vt ra ngoi gii hn cho php v khng c vin no vt gp i sai s cho php. 2.3. nh tnh : Lc 1 lng bt vin tng ng 0,2g Amoxicilin vi 2ml nc trong 5 pht. Lc ra cn lc u vi ethanol, sau vi ether, ly khong 2mg cn thu c, lm m bng 0,05ml nc v thm 2ml dung dch Formaldehyd trong acid sulfuric. Dung dch thc t khng mu, un trong cch thy 1 pht, dung dch c mu vng sm. 2.4. Nc : Khng c qu 14,5% dng trong phng php Karl Fisher. Dng khong 0,1g bt thuc. 2.5. tan r : Khng c qu 30 pht. Th trn 6 vin nhit 36-380C, c 6 vin u phi tan r trong thi gian quy nh. 2.6. nh lng : Nang Amoxicilin 500mg phi cha Amoxicilin (C16H19N3O5S) t 92,5% n 110,0% hm lng ghi trn nhn.

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- Tin hnh : Dung dch th, cn 1 khi lng bt vin tng ng 0,1g Amoxicilin vo bnh nh mc 100ml, thm nc ct khong 60ml v lc cho tan hon ton, thm nc n vch, trn u. Lc, b khong 20ml dch lc u. - Ly chnh xc 2,0ml dch lc cho vo bnh nt mi dung tch 100ml, thm 2,0ml dung dch NaOH 1N, lc u yn 15 pht. Chun Iod tha bng dung dch Natri Thiosulfat 0,01N cho n khi mt mu. Ch th l h tinh bt cho vo lc mi nh lng. Ghi nt ml. Ly 2ml dung dch th cho vo bnh nn dung tch 100ml khc, thm 0,12ml acid hydroclorid 1N v 10ml dung dch Iod 0,01N. Chun ngay bng dung dch Natri Thiosulfat 1N cho n khi mt mu. Ghi Nt ml. Dung dch chun : Cn chnh xc 1 lng bt Amoxicilin chun khong 0,1g trong bnh nh mc 100ml, ha tan vi nc va 100ml. Song song tin hnh ghi dung dch th. Hm lng % Amoxicilin c tnh theo cng thc : Nt - nt Pc m C% = * * C% * * 100 Nc - nc Pt 500mg Trong : Pt l khi lng bt th cn chun Pc l khi lng bt chun cn C% l hm lng bt Amoxicilin chun.

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Bi 11 : KIM NGHIM VIN NN SULFAGUANIDIN 500MG

1. Nguyn tc : Sulfaguanidin c chun bng dung dch Natri nitrit theo phn ng diazo ha, im tng ng c th c xc nh bng ch th ni, giy tm h tinh bt c KI hoc o in th. 2. Kim nghim : 2.1. Tnh cht : Vin nn mu trng, mt vin nhn, cnh v thnh vin phi lnh ln. Th bng cm quan, mu th phi t cc yu cu trn. 2.2. ng u khi lng : Khi lng trung bnh vin m 5% Xc nh trn 20 vin bt k, khng c qu 2 vin ra ngoi sai s cho php v khng c vin no nm ngoi gii hn gp i sai s cho php. 2.3. tan r : Khng c qu 15 pht. Th trn 6 vin, tt c phi r trong khng qu 15 pht. 2.4. nh tnh : a. Ly 1 lng bt vin tng ng vi 200mg sulfaguanidin, thm 5ml dung dch NaOH 10%, un si s c hi amoniac bay ra (lm xanh giy qu thm t bt trn ming ng nghim). b. Ly 1 lng bt vin tng ng vi 50mg sulfaguanidin, thm 2ml HCl 10%, lc k, lc, lm lnh dch lc trong nc , thm 4ml dung dch Natri nitrit 1%, lc u, ly 1ml dung dch thu c, thm 5ml dung dch 2Naphtol trong kim cho ta thm. 2.5. nh lng : Vin nn Sulfaguanidin 500mg phi cha Sulfaguanidin C7H10N4O2S.H2O t 95,0% n 105,0% hm lng ghi trn nhn. Cn 20 vin, tnh khi lng trung bnh vin, nghin thnh bt mn, cn chnh xc 1 lng bt vin tng ng vi khong 0,200g sulfaguanidin, cho vo bnh nn nt mi 100ml, thm 15ml dung dch HCl 25% v 15ml nc, lc k. Thm vo mi bnh 2g Kali bromid v lm lnh n khong 10 0C ri chun bng dung dch Natri nitrit 0,1N. Ch th : 2 git Tropeolin 00 + 1 git xanh methylen Giy tm h tinh bt c KI in th k 1ml dung dch Natri nitrit 0,1N tng ng vi 23,27mg C7H10N4O2S.H2O Hm lng phn trm ca vin c tnh theo cng thc :
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n(ml) * k * 23,27 * m * 100 C% = 500(mg) * p Trong : m l khi lng trung bnh vin (mg) P l khi lng bt cn nh lng (mg)

Bi 12 : KIM NGHIM THUC TIM VITAMIN B12

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1000mcg (ng 1ml)

1. Tnh cht : ng hn kn, nhn ng quy ch, ng dung dch trong mu mn. 2. ng u th tch : Phi t t 1,0 - 1,15ml. Kim tra trn 5 n v bng bm kim tim sch, kh, cc ng thuc phi c th tch nm trong gii hn quy nh. 3. nh tnh : Ph hp th ca dung dch mu th ghi c phn nh lng phi c 3 cc i hp th 278 1nm, 361 1nm v 550 2nm. T s hp th 278nm v 550nm so vi hp th 361nm l 0,57 0,02 v 0,3 0,02. 4. pH : 4,0 - 5,5 Cho khong 30ml dung dch ch phm vo cc c m 50ml v xc nh pH ca ch phm bng pH meter, pH ca dung dch phi nm trong khong quy nh. 5. nh lng : Dung dch tim vitamin B12 1000mcg ng 1ml phi cha Cyanocobalamin (C63H88CoN14O14P) t 95,0 n 115,0% hm lng ghi trn nhn. - Tin hnh : Pha long ch phm vi nc c nng 25mcg trong 1ml. o hp th bc sng 361nm vi cc o dy 1cm, mu trng l nc ct. - Tnh hm lng Cyanocobalamin (C63H88CoN14O14P) theo A (1%, 1cm), ly 207 l gi tr ca A (1%, 1cm) cc i 361nm. At At C% = = .n A(1%,1cm) A(1%,1cm) Trong n l pha long.

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