I HC Y DC TP.

H CH MINH KHOA KHOA HC C BN-B MN SINH HC MN DI TRUYN HC

SINH SN V TNH NG VT A BO

TO DNG NG VT

Ths. L Th L Uyn

To dng cp phn t
*To dng in vitro:PCR *To dng in vivo

- Ct - Ni - Chp gen

Ct gen
Dng enzim ct c hiu Restrictase: -Tch on DNA chn

- Ct th truyn (plasmid, phage ,) nhng im xc nh.

a on DNA ct vo th truyn

a DNA ti t hp vo t bo vi khun nhn (E.coli) v tin hnh nui cy to dng gen

NHN BN V TNH
Nhn bn phi ngi v ng vt c th xy ra trong t nhin hoc nhn to.

sinh i cng trng

Vinchnnui quc gia T Lin, H Ni, 2005

Film human cloning

LCH S NHN BN V TNH

Ngy 5/7/1996 - Ian Wilmut v Keith Campbell vin Roslin, Scotland nhn bn thnh cng cu Dolly t cc t bo tuyn v ca mt con cu m.

Sau s kin Dolly, Ian Wilmut v cng s tip tc to ra hai con cu u tin c mang gen ngi t tn l Polly v Molly.

LCH S NHN BN V TNH

Nm 2005, Hwang Woo Suk to dng 1 ch ch snuppy t tai ca 1 con ch sn c 3 tui

Ct phi lm i
(a)Ct phi giai on phi du gi (b)Ct phi giai on blastocyst sm

K thut nhn bn bng chuyn nhn t bo soma (somatic cell nuclear transfer)

Quy trnh nhn bn bng chuyn nhn t bo soma (somatic cell nuclear transfer) Ht b nhn n bi t bo trng ca c th m

Hnh . Loi nhn t bo trng

Quy trnh nhn bn bng chuyn nhn t bo soma (somatic cell nuclear transfer-SCNT)

Dolly Parton

Cu Dolly c mt trng v lng xon mu kem

1998, Dolly giao phi vi David (x Wales)- tp ch Science News. => 13/04/1998 sinh mt cu ci Bonnie. => 24/03/1999, Dolly tip tc sinh thm hai cu c v mt cu ci kho mnh. 14/02/2003, Dolly cht v bnh vim phi.

K thut to dng Hono lulu

NHN BN TR LiU

 ngha to dng ng vt
- Mc ch ca vic to dng gen ng vt c v (c bit l ngi): to ra cc sn phm ng nh trong c th vi s lng ln v c gi tr thng mi. - To 1 dng gen ng ngha vi vic thu nhn mARN v Protein ca gen . DNA mARN Protein

 ngha ca vic to dng ng vt

To dng t bo ng vt tng lng t bo ging nhau v cht liu di truyn Trong cng tc ging: to i con ci hon thin di truyn Dng ha cc ging th c nguy c cao (phc hi cc ging c nguy c tuyt chng).

Thng 12/2006 FAD (Food and Drug Administration) cng b bn d tho trong c kt lun rng tht v sa ca ng vt sinh sn v tnh l an ton i vi ngi tiu th.

I HC Y DC TP. H CH MINH KHOA KHOA HC C BN-B MN SINH HC MN DI TRUYN HC

T BO GC NG DNG V TRIN VNG

Ths. L Th L Uyn

3.1.a. T bo gc ton nng hay t bo gc thy t (totipotent stem cells):

3.1.b. T bo gc vn nng (pluripotent stem cells):

- C kh nng bit ha thnh tt c cc t bo ca c th c ngun gc t ba l phi. -TBG vn nng:

Khng to nn c mt c th sinh vt hon chnh Ch c th to nn c cc t bo, m nht nh. Cc t bo inner cell mass-ICM l nhng t bo gc vn nng.

3.1.c. T bo gc a nng (multipotent stem cells):

-L nhng t bo c kh nng bit ha thnh nhiu loi t bo ca c th t mt t bo ban u. -VD: Hematopoietic cell

3.2.a. T bo gc phi (Embryonic stem cells-ES)

S pht trin ca Blastocyst

ICM

Film D:\dai hoc y duoctp.hcm\march-04-2011\picture-a\phat trien phoi nguoi.flv

3.2.c.T bo gc ung th biu m phi (Embryonic carcinoma-EC) - c thu nhn t cc khi u ca tinh hon, bung trng mt s chng chut. - c to ra t cc t bo mm EG - EC c th bit ha thnh nhiu kiu t bo khc nhau trong cc m.

Quy trnh thu nhn mu cung rn ca ngn hng quc t ng lnh M t ti Florida (Cryobanks International, Inc)

Thao tc thu mu t dy rn-Ti mu sau khi thu nhn phim1 phim2

4 . Cc ngun ly t bo gc:

4.3. Ngun ly t bo gc trng thnh: Thng ly t cc t chc trng thnh nh: mu cung rn, trung m cung rn, ty xng, mu ngoi vi, nang lng, t chc no

ng dng t bo gc trng thnh trong iu tr Nhn bn tr liu

ng dng t bo gc trng thnh trong iu tr

- iu tr cc bnh t min, tai bin mch mu no,tn thng gic mc, cc bnh mu v bnh gan, tn thng ty sng, lin vt thng da, iu tr ung th: ung th tinh hon, a u ty, l-x-mi, ung th v, u nguyn bo thn kinh, u lympho Non-Hodgkin, carcinoma t bo thn, ti to c tim sau cn au tim, i ng type I, tn thng xng v sn, bnh Parkinson

Cc nh khoa hc th gii va thnh cng trong vic cy ghp kh qun cho mt bnh nhn sut na b ct b mt bn phi.

kh qun b tn thng

Kh qun lai c to ra t kh qun ca ngi l v t bo ca ch Claudia

Claudia Castillo, 30t ngi u tin trn th gii khng phi dng thuc chng o thi sau khi ghp b phn mi vo c th.

Claudia Castillo vi bc s phu thut Paolo Macchiarini trc khi ln bn phu thut.

Ti liu tham kho Cng ngh sinh hc trn ngui v ng vt ; Phan Kim Ngc, Phm Vn Phc; Nh xut bn gio dc Vit Nam. Cng ngh t bo gc; Phan Kim Ngc, Nh xut bn gio dc Vit Nam. Ti liu Di truyn hc, i hc y dc TP HCM Khoa khoa hc c bn b mn sinh hc, L Thy Quyn, 20092010.

 http://www.sinhhocvietnam.com/vn/module s.php?file=article&name=News&sid=971 http://www.oicr.on.ca/Portalnews/Vol3_I ssue3/lifetime.htm http://news.bbc.co.uk/2/hi/7735696.stm www.stemcellresearch.org

Ti liu tham kho

Animal Transgenesis and cloning; LouiseMarie Houdebine, 2003 John Willey and Sons, Ltd Stem cells handbook, Stewart Sell, MD;Humana Press Inc, Totowa, New Jersey, 2004. Genetic engineering applications in animal breeding- Hugo H.Montaldo, Vol.9 No.2, Issue of April 15,2006