B CNG THNG TRNG I HC CNG NGHIP THC PHM KHOA CNG NGH THC PHM

BO CO THC HNH
Mn GVHD
: :

Kim tra cht lng Tht inh Hu ng

Nhm 1

Nhm thc hin :

LP 04CDNKN2 Nin kha: 2011 2014 Tp.H Ch Minh

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

LI M U
Knh cho thy, y l bi bo co ghi li ton b cng vic v kin thc hc c trong mn Kim tra cht lng Tht ca Nhm 1 trong thi gian thc hnh qua. Nhm 1 cm n thy inh Hu ng tn tnh ch dy chng em trong qu trnh hc tp v thc hnh, cn nhiu thiu st trong qu trnh hc cng nh trong qu trnh lm bo co nn mong thy b qua v cho chng em li nhn xt cng nh gp hon thin hn v mt kin thc trnh by trong bi bo co ny. Mt ln na xin cm n thy, chc thy nhiu sc khe v thm nhiu thnh cng trong cng vic ging dy.

Danh sch Nhm 1

H v tn Phng Thin Tm (NT) Nguyn Th Ngc Nhung L Th Hng Phng L Th Thu Phng Nguyn Th Kim Tho M s sinh vin 3305110568 3305110468 3305110516 3305110517 3305110616 Mc lm vic 100% 90% 90% 90% 90%

GVHD inh Hu ng 2

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

BI 1: Kim tra cht lng tht ti ------------------1.1 Phm vi p dng: Tiu chun ny p dng cho tht gia sc, gia cm v tht chim, th nui trng thi ti dung lm thc phm. 1.2 Dng c, thit b v ha cht:

- Dao - Tht - Ni inox - R - Bp in - T nha - a - Mu tht g, b, heo - Cn k thut v cn phn tch 1.3 Tin hnh - Quan st mu v nh gi cc ch tiu v trng thi, mu sc , mi, v, n hi, ty xng (nu c) , trng thi m (nu c). - Luc tht ( 100oC, thm NaCL 0.5 ~ 0.7% cn bng p sut) v nh gi nc luc tht v vng m, mi, c c, git m, bt.

GVHD inh Hu ng 3

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

- Lp bng tnh im cm quan theo thang im (5). 1.4 c kt qu:

nh gi cht lng tht heo: - nh gi cm quan: Thang im Rt tt Ch tiu Mu sc Mi Trng thi n hi Mu sc: Rt tt: c mu ti Tt: hi Trung bnh: hi ng mu sm Khng tt: lt, hi thm Km: lt hn v thm nhiu hn Mi : Rt tt: mi tanh t nhin ca tht heo x x x x Tt Trung bnh

Khng tt

Km

GVHD inh Hu ng 4

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Tt: mi tanh hi mc va phi Trung bnh: mi tanh hi nng hn Khng tt: mi hi nng hn Km: mi thi xut hin r rt Trng thi: Rt tt: lin kt gia cc si c, cc m cht ch Tt: lin kt hi ri rc Trung bnh: lin kt c tnh ri rc Khng tt: lin kt ht cht ch, c xu hng t ri hoc v khi c tc ng mnh Km: lin kt li rc, d b t ri n hi: Rt tt: vt lm phc hi rt nhanh (khong 5 giy) Tt: vt lm phc hi nhanh (khong 10 giy) Trung bnh: mc phc hi chm Khng tt: phc hi nhng khng tr li nh hnh dng ban u Km: khng phc hi c

GVHD inh Hu ng 5

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

- nh gi nc luc tht (mNaCl = 0.65g): Thang im Rt tt Ch tiu Vng m Mi v c Git m B t Vng m: Rt tt: xut hin t, mu sc trong Tt: mu sc c Trung bnh: hi ng mu Khng tt: ng mu, xut hin nhiu Km: mu c, xut hin rt nhiu Mi v: Rt tt: mi thm Tt: mi thm nh Trung bnh: mi thm c ln mi hi Khng tt: c mi hi x x x x Tt Trung bnh x

Khng tt

Km

GVHD inh Hu ng 6

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Km: mi hi nng nc c: Rt tt: nc luc trong Tt: hi trong Trung bnh: hi c v ng mu Khng tt: c Km: c v ng mu Git m: Rt tt: git m trong, to, trn, c mu c trng Tt: kch thc va, trong, trn Trung bnh: kch thc nh, hi c Khng tt: kch thc nh hn, c Km: c, khng quan st r Bt: Rt tt: rt t, mu trng, gn nh khng c Tt: c xut hin v mu trng Trung bnh: xut hin nhiu, ng mu hi xm Khng tt: nhiu, c mu xm Km: nhiu, c mu xm en

GVHD inh Hu ng 7

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

nh gi cht lng tht b: - nh gi cm quan: Thang im Rt tt Ch tiu Mu sc Mi Trng thi n hi Mu sc: Rt tt: mu ti, sng Tt: ti Trung bnh: hi sm Khng tt: sm Km: sm en Mi : Rt tt: tanh c trng ca b Tt: mi tanh nh Trung bnh: mi tanh ln mi hi x X X Tt Trung bnh x

Khng tt

Km

GVHD inh Hu ng 8

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Khng tt: mi hi r rt Km: mi hi thi nng Trng thi: Rt tt: lin kt gia cc m v c cht ch Tt: lin kt hi cht ch Trung bnh: lin kt khng cht ch lm Khng tt: lin kt khng cht ch Km: lin kt d dng b pht v bi tc ng n hi: Rt tt: vt lm phc hi rt nhanh (khong 5 giy) Tt: vt lm phc hi nhanh (khong 10 giy) Trung bnh: mc phc hi chm Khng tt: phc hi nhng khng tr li nh hnh dng ban u Km: khng phc hi c

GVHD inh Hu ng 9

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

- nh gi nc luc tht (mNaCl = 0.58g): Thang im Rt tt Ch tiu Vng m Mi v c Git m B t Vng m: Rt tt: nh vng, trong Tt: nh vng sm Trung bnh: vng m tha, khng c mu Khng tt: bm thnh ni, mu hi sm Km: vng m c v ri rc Mi v: Rt tt: thm c trng ca tht b Tt: thm nh Trung bnh: ch ngi thy khi gn nc luc Khng tt: c mi l X X X x x Tt Trung bnh

Khng tt

Km

GVHD inh Hu ng 10

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Km: c mi l nng nc, khng cn mi thm c: Rt tt: trong, khng c cn Tt: trong, c cn li ti Trung bnh: hi c Khng tt: c Km: c v nhiu cn Git m: Rt tt: to, trn, sng Tt: to va, trn, sng Trung bnh: va, trn, hi sng Khng tt: nh Km: nh li ti, hi ng mu Bt: Rt tt: t bt, c mu trng sng Tt: bt va, c mu trng Trung bnh: va, c mu hi c Khng tt: nhiu, c Km: rt nhiu, c mu c ng en

GVHD inh Hu ng 11

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

nh gi cht lng tht g: - nh gi cm quan: Thang im Rt tt Ch tiu Mu sc Mi Trng thi n hi Ty xng Tri thi m Mu sc: Rt tt: c mu vng ti Tt: vng nht Trung bnh: vng sm Khng tt: vng sm, ng sang Km: mu cam sm Mi : Rt tt: tanh c trng x x x x x x Tt Trung bnh

Khng tt

Km

GVHD inh Hu ng 12

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Tt: mi tanh nh Trung bnh: tanh v hi Khng tt: hi, khng c mi tanh Km: mi i thiu xut hin Trng thi: Rt tt: cc m v c lin kt cht ch Tt: lin kt tt Trung bnh: khng cht ch lm Khng tt: khng cht ch Km: ri rc n hi: Rt tt: vt lm phc hi rt nhanh (khong 5 giy) Tt: vt lm phc hi nhanh (khong 10 giy) Trung bnh: mc phc hi chm Khng tt: phc hi nhng khng tr li nh hnh dng ban u Km: khng phc hi c Ty xng: Rt tt: hi c, c mu, vn cn lin kt cht, cha r Tt: c, c du hiu r

GVHD inh Hu ng 13

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Trung bnh: hi r v c nc chy ra Khng tt: r Km: r v c mi hi Trng thi m: Rt tt: dnh cht vo tht, c mu vng ti Tt: mu vng nht Trung bnh: ng mu vng sm Khng tt: vng sm Km: sm, c - nh gi nc luc tht (mNaCl = 0.58g): Thang im Rt tt Ch tiu Vng m Mi v c Git m B t Vng m: Rt tt: trong, c mu vng x x x x x Tt Trung bnh

Khng tt

Km

GVHD inh Hu ng 14

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Tt: hi trong, mu vng nht Trung bnh: c, hi ng mu Khng tt: c Km: rt c v mu sm Mi v: Rt tt: mi thm Tt: thm nh Trung bnh: t thm, c mi hi Khng tt: khng cn mi thm Km: c mi hi nng c: Rt tt: trong, khng c cn Tt: hi trong Trung bnh: hi c Khng tt: c Km: rt c v c cn Git m: Rt tt: to, trn, c mu vng Tt: va, mu vng nht

GVHD inh Hu ng 15

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Trung bnh: nh, mu sm Khng tt: nh, ri rc, mu sm Km: nh li ti Bt: Rt tt: t, trng sng Tt: t, c mu trng sm Trung bnh: va, c mu trng ng vng Khng tt: nhiu, mu hi sm Km: rt nhiu, ng mu xm nh gi: c 3 loi tht u c trng thi tt v c th s dng c.

GVHD inh Hu ng 16

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

BI 2: o pH, nh tnh H2S, NH3 ------------2.1 2.1.1 Xc nh pH: Phm vi p dng: Tiu chun ny qui nh phng php chun gi tr pH trong tt c cc loi tht v cc sn phm ch bin t tht gia sc, gia cm. 2.1.2 Dng c, thit b v ha cht:

- Cn k thut - My xay tht - Cc 50ml - a thy tinh - Phu 8cm - Giy lc - Bnh tia - Nc ct - KCl 0.1N - pH k - Giy o pH - Mu tht b v tht heo

GVHD inh Hu ng 17

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

2.1.3

Tin hnh:

- Dng giy pH: dn giy pH trc tip ln b mt tht, quan st giy pH c i mu hay khng sau 10-15 pht. - Dng my o pH: cn 5g tht, xay v ng ha mu bng nc ct, lc bng giy lc hoc bng gn. Ht 10ml dch lc v thm KCl 0.1N vi lng bng 10 ln mu. iu chnh pH k v o dch mu. Sau khi o phi v sinh k pH k. 2.1.4 c kt qu:

pH khi o bng giy pH: - tht heo: 6 - tht b: 6 pH khi o bng pH k: - tht heo: 4.8 - tht b: 4.9 Nhn xt: kt qu ca 2 cch o sai lch l do qu trnh lc khi chun b mu o bng pH k. 2.2 2.2.1 nh tnh H2S: Phm vi p dng:

GVHD inh Hu ng 18

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Tiu chun ny xc nh ti i ca tht gia sc, gia cm, tht chim c dng lm thc phm. 2.2.2 Dng c, thit b v ha cht:

- Cn k thut - My xay - a petri - Cc 100ml, 250ml - a thy tinh - Acid photphoric 5% - Giy ch acetat 2.2.3 Tin hnh:

- Cn 20g tht, xay nhuyn v cn 5g mu xay dn u ln 2 mt ca a petri, dn giy ch acetat ln b mt mu, sau 15 pht quan st 1 ln. Nu giy ch acetat xut hin chm en => c H2S, nu khng c chm en => khng c H2S.

GVHD inh Hu ng 19

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Mu tht heo

Mu ch

GVHD inh Hu ng 20

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

2.2.4

c kt qu: Giy ch acetat khng xut hin chm en, vy mu tht heo v tht b

khng c H2S chng t tht cn ti. 2.3 2.3.1 nh tnh NH3: Phm vi p dng: Tiu chun ny dng s bc hi ca HCl trong dung dch hn hp cn ethanol phn ng xy ra vi NH3 v to thnh mui clorua amoni (xut hin khi trng). 2.3.2 Dng c, ha cht v thit b:

- ng nghim c nt y - Dao - Tht - Gi ng nghim - Mc treo tht - Mu tht heo - Dung dch Herber 2.3.3 Tin hnh:

GVHD inh Hu ng 21

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

- Cho vo ng nghim 5ml dung dch Herber, ct tht thnh lt mng sau mc vo mc treo tht, vo trong ng nghim cch b mt dung dch t 1-2cm. Sau mt thi gian quan st xem trn thnh ng nghim c khi trng hay khng, nu c => c NH3 v ngc li.

Mu tht 2.3.4 c kt qu: Trn thnh ng nghim c lp khi trng m chng t tht c b i v khng cn ti mc va phi.

GVHD inh Hu ng 22

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

BI 3: Phn ng sa lng Protein, nh tnh Borax --------------------3.1 3.1.1 Phn ng sa lng Protein: Phm vi p dng: Tiu chun ny xc nh ti, i ca tht gia sc, gia cm, tht chim c dng lm thc phm da trn phn ng CuSO4 ha tan Protein trong mu. 3.1.2 Dng c, ha cht v thit b:

- My xay tht - ng ong 50ml - Giy lc - a thy tinh - Cc 100ml - Bnh nh mc - Pipet 1ml - Nc ct - Bnh tam gic - CuSO4 1% - Mu tht heo

GVHD inh Hu ng 23

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

3.1.3

Tin hnh:

- Cn 12,5g tht xay, cho vo bnh nh mc 250ml v nh mc ti vch, yn trong 15 pht sau em i lc.Chun b 2 cc 100ml, cc 2 ht 5ml dch lc, cc 1 ht 5ml dch lc + 5 git CuSO4 1%. trong 5 pht v nhn xt c ca cc 1 (c cho CuSO4 1%) so vi cc 2.

Mu tht 3.1.4 c kt qu: Dung dch trong cc 1 (c cho CuSO4 1%) trong hn dung dch trong cc 2. Da trn nguyn l lng protein b ha tan bi CuSO4 nn nu tht cn ti v khng b i th lng protein b ha tan s t. Nh vy mu tht

GVHD inh Hu ng 24

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

khng cn ti (thi gian t lc git m n lc kim tra khong 5 gi) nhng vn c th s dng c. 3.2 3.2.1 nh tnh Borax: Phm vi p dng: Xc nh tht v cc sn phm tht c Borax hay khng. 3.2.2 Dng c, ha cht v thit b:

- Cn phn tch - Giy thm ngh - Pipte 1ml, 5ml - Bnh nh mc 250ml - Phu thy tinh - a petri - Gi pipet - Giy lc - HCl 5N - Mu tht heo v mu ch la 3.2.3 Tin hnh:

- Cn mu xay (mtht = 25.12g, mch = 12,57g) cho vo bnh nh mc 250ml v nh mc ti vch. Sau 15 pht em i lc, ht 5ml dch lc

GVHD inh Hu ng 25

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

cho vo a petri + 2ml HCl 5N. Nhng giy ngh vo dung dch trong a, ri kh, sau 15 pht quan st v c kt qu. Giy ngh khng i mu => khng c borax, giy ngh i sang mu hoc hng => c borax.

Mu v giy ngh 3.2.4 c kt qu: Mu ch c Borax. Mu tht heo khng c Borax.

GVHD inh Hu ng 26

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

BI 4: Kim tra cht lng hp ----------4.1 Phm vi p dng: hp tht cc loi hoc cc hp cc sn phm t tht. 4.2 Ha cht, dng c v thit b:

- Km - Cc 250ml - Mung inox - Kp o - Cn k thut - Giy o pH - Bp in - Ni inox - Mu hp 4.3 Tin hnh: Kim tra cc ch tiu theo th t t ngoi vo trong, do gii hn thi gian v iu kin phng th nghim nn ch kim tra c 1 s ch tiu, cc ch tiu cn li ch hc trn l thuyt.

GVHD inh Hu ng 27

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Mu hp 4.3.1 Kim tra bn ngoi:

- Hn s dng, ngy sn xut, thnh phn, khi lng, ni sn xut, tn sn phm, m vch, thnh phn, ch tiu cht lng. - Kim tra np v y (trng thi) - kn (g hp, ngm nc nng) 4.3.2 Kim tra bn trong:

GVHD inh Hu ng 28

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

- Cm quan (mi v, mu sc) - Khi lng tnh (% khi lng, t l ci nc) - Vch hp (lp vecni) - Ha hc (pH; NaCl, acid, cht bo qun; kim loi nng; kim tra S t joint cao su trong m ghp) - Ha sinh (vi sinh vt) - Ha l ( chn khng, m ghp) 4.4 c kt qu:

Kim tra bn ngoi: - Tn sn phm: Heo hai lt (Two bits of pork) - Cng ty sn xut: 3 BNG MAI - Sn xut ti Cng ty TNHH mt thnh vin Vit Nam k ngh sc sn (VISSAN) - Thnh phn: Nc heo: 34% M heo Protein u nnh Tinh bt bp ng Mui Tiu
GVHD inh Hu ng 29

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

Hnh Ti Cht n nh (452) Cht iu v (621) - Ch tiu cht lng ch yu: Protid 7% weight NH3 < 4 mg/100grs - Ngy sn xut: 09/11/2012 VISSAN - Hn s dng: 09/11/2015 04HO50 - M vch: 8934572002006. y l m vch thuc h thng m s EAN loi s dng 13 s (EAN-13) c cu to t tri sang phi nh sau: M quc gia (ca Vit Nam l 893): hai hoc ba s u tin M doanh nghip: t bn, nm hoc su s. M mt hng: c th l nm, bn hoc ba s ty doanh nghip. S kim tra: thng l s cui cng - Khi lng tnh: 150g ( 10g) - Np v y: bnh thng, khng mp mo - Trng thi: khng b phng - kn: G m thanh: m thanh nghe trong, thanh l hp tt

GVHD inh Hu ng 30

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

kn: un si nc 70 - 80OC (nu si hn s c bt kh v kh c kt qu) sau cho hp mu vo v quan st xem c bt kh hay khng. Khng c bt kh => kn. Kim tra bn trong: - Cm quan: Mu sc: hng, c trng ca sn phm Mi: thm V: mn du, ngt Trng thi sn phm: cn nguyn, khng b v - Khi lng tnh: Khi lng tnh 102,68g so vi 150g trn bao b T l ci nc 60:40 so vi 50:50 trn bao b - Vch hp: lp vecni khng b try xt, khng b r st, khng b nm v sn phm bm vo - pH: o c bng giy o pH l 6 - Ha l (kim ra m ghp): dng km ct ri phn thn v np o di mc thn v di mc np. Sau tnh ton theo cng thc cho v so snh kt qu vi bng tiu chun m ghp nh gi. - Di y l bng tiu chun ghp m dnh cho sn phm hp quy cch 307:

GVHD inh Hu ng 31

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

307 Gii hn bnh thng chp nhn su ca m (mm) rng (mm) dy (mm) Mc thn(mm) Mc np (mm) ph thc t (mm) % cht (%) % mc thn (%) Ti a khong trng (mm) ng p lc 3.10 - 3.40 2.85 3.15 1.0 1.21 1.85 2.20 1.85 2.20 Thp nht 1.02 mm 70 90% Thp nht 70% 0.01 0.22 Tt Gii hn ti a chp nhn 2.09 - 3.50 2.65 3.25 0.80 1.31 1.65 2.30 1.65 2.30 Thp nht 1.02 mm 70 90% Thp nht 70% 0.01 0.22 Tt

di m ghp, k hiu l SL (Seam Length) = 2.8 mm

GVHD inh Hu ng 32

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

dy m ghp, k hiu l ST (Seam Thickness) = 1.5 mm

di mc thn, k hiu l BH (Body hook) = 2 mm

GVHD inh Hu ng 33

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

di mc np, k hiu l CH (Cover hook) = 1.8 mm

di mc thn, k hiu l BH (Body hook) = 2 mm dy st lm np, k hiu l Te (0.28 ~ 0.3) = 0.3 mm dy st lm thn, k hiu l Tb (0.2 ~ 0.24) = 0.2 mm ph thc t ca m ghp = BH + CH + Te + SL = 6.9 mm % chng m =
% mc thn =

= 65% = 92.7%

Khong trng = ST (2Tb + 3Te) = 0.2 mm Nhn xt: cc ch tiu nh gi m ghp c sai s do thao tc o v c kt qu khng chun xc nn khi so snh vi bng tiu chun khng khp.

GVHD inh Hu ng 34

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

BI 5: Xc nh hm lng NH3 -----------------5.1 Phm vi p dng: Xc nh hm lng NH3 trong tht v cc sn phm t tht. Nguyn l: dng s bc hi ca acid trong dung dch hn hp phn ng xy ra vi NH3 v to thnh mui. Mc ch: xc nh NH3 trong thc phm nh gi h hng ca thc phm v nh gi ng gi tr v protid v acid amin ca thc phm. 5.2 Dng c, thit b v ha cht: - My xay - Cn k thut - Bp in gia nhit bnh cu - Bnh tam gic - Bnh cu - H thng chng ct - Thau nha - Cc 100ml - Pipet

GVHD inh Hu ng 35

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

- Bnh tia - Gi pipet - ng nghim - Buret - Gi buret - Qu bp cao su - MgO - H2SO4 0.1N - Metyl Red - NaOH 0.1N - Giy qu - Mu tht heo - PP 1% - Nc ct 5.3 Tin hnh: Cn 5.06g mu tht heo xay nhuyn cho vo bnh cu + 50ml nc ct, thm vo 0.5ml PP1%, thm MgO bo ha cho n khi dung dch trong bnh cu c mu hng bn th dng li. Lp b chng ct sau un nng bnh cu nhit va phi trnh dung dch trong bnh cn v kht.

GVHD inh Hu ng 36

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

H thng chng ct t lp t Bnh tam gic chun b sn 20ml H2SO4 0.1N + 5 git Metyl red. Dng bnh tam gic hng dung dch chng cht c t b chng ct. Sau khong 30 pht dng giy pH th xem qu trnh chng ct kt thc hay cha. Dng giy pH chm th vo u ra ca b chng ct, nu c mu xanh -> vn cn NH3 v tip thc chng ct, nu khng i mu ->

GVHD inh Hu ng 37

Nhm 1

Trng i hc Cng nghip thc phm TP.HCM Khoa Cng ngh thc phm

Kim tra cht lng Tht

ht, dng qu trnh chng ct m mang bnh tam gic i chun xc nh hm lng NH3. Chun bng NaOH cho n khi dung dch chuyn sang mu vng nht th dng v ghi li th tch NaOH tiu tn. 5.4 Tnh kt qu:

= 16.8 (g/ml) Vi: V1: th tch H2SO4

V2: th tch NaOH tiu tn 0.0017: s g NH3 tng ng trong 1ml H2SO4 m: khi lng mu Nhn xt: kt qu cha ng tin cy do cn sai st trong qu trnh chng ct v chun .

GVHD inh Hu ng 38

Nhm 1