Ecophysiological characterization of zebra mussel

populations (Dreissena polymorpha) invading the

Ebro and the Jcar hydrographic basins.
basins
Roco Urea, Olga Ruiz, Jose del Ramo, Miguel ngel
Lpez, Carlos Barata, Amparo Torreblanca

 CEBRAPOPprojectdealswiththestudyofthegenetic
structureandtheecophysiologicaldifferentiationdegreeof
the Spanish zebra mussel populations in response to the
theSpanishzebramusselpopulationsinresponsetothe
environmentalandanthropogenicstress.

Thestudyincludespopulationssampledatdiverselocations,
differingintheirwaterquality,fromtheEbroandJcarbasins.

 General indexes of health status

Enzime activityy related to ion transport
p and
osmoregulation.

Chemical analysis of essential and toxic metals

metals.
Proteomic analysis

MARCHANDOCTOBER2009

TISSUECONDITIONINDEX:
TISSUE
CONDITION INDEX:
softtissue(dryweight)/shellvolume

TISSUECONDITIONINDEX:
TISSUE
CONDITION INDEX:
softtissue(dryweight)/shellvolume

TISSUECONDITIONINDEX:
TISSUE
CONDITION INDEX:
softtissue(dryweight)/shellvolume

%DRYWEIGHT/WETWEIGHT

%DRYWEIGHT/WETWEIGHT

Chemical analysis of tissues

Element

Tissue

(g g-1 ww)

March
Sitjar
b 0.21 0.01

Caspe1
c
0.34 0.02

Cd

dg
gills (x10 -2)

bc

cd

Hg

dg (x10 -22 )

ad

Pb

gills (x10 -2 )
dg

add1.31 0.42
c 0.56 0.11

Cu
Zn
K
P
Fe
Mn
Ca

gills (x10 -2 )
dg
gills
dgg
gills
dg
gills
dg
gills
dg
gills
dg
gills
dg
gills

1.33 0.11

d 0.37 0.10
a

8.06
ab 1.36
a
1.45
a
15.89
a
9.84
a 398.75
a 314.03
a
693.17
a
387.28
a
62.39
b
49.54
ab3.21
a1.72
b 609.74
abc
353.16

1.74
0.32
0.55
0.94
1.74
36.98
30.11
134.40
72.01
11.18
15.46
0.60
0.55
170.03
112.42

1.06 0.23
1.46 0.39

0.65 0.06
bc
0.08 0.06
ab

1.96
abc
1.38
a
1.05
a
16.80
a
11.50
e
1369.18
ab
361.48
d
1292.49
ab458.62
ab
85.77
a
20.83
abb
3.45
a
1.42
ab
516.59
ab
153.98

3.12
0.25
0.34
1.26
0.65
79.41
21.31
98.28
73.50
28.71
7.34
0.57
0.39
83.28
38.05

Ribarroja
bc
0.25 0.03
de 0.82 0.12
a 2.14 0.26
d 0.44 0.07
ab

0.03 0.02

0.41
bcd 2.01
a 1.19
a
15.67
a
10.71
de
1223.45
bc
412.21
cd
1160.22
ab 510.46
b
88.34
a
17.78
abb
4.54
a
1.66
ab
351.72
ab
179.54

0.26
0.42
0.22
1.08
1.30
89.29
36.27
92.85
33.77
6.40
2.80
1.46
0.52
81.41
38.37

Flix Factory
d 0.98 0.14
a

7.17 0.33

b 14.91 1.80
a 6.86 0.95
bcd0.10 0.05
b

0.41
d
2.13
a 1.00
a
16.42
a
9.69
bc
1046.49
a
272.40
bc
1007.12
a 418.02
b
147.89
a
20.93
abb
5.79
a1.93
b 598.45
a 185.43

0.23
0.34
0.26
1.39
0.88
70.44
29.31
70.37
46.02
36.38
3.64
1.55
0.49
102.46
50.40

Mequinenza
0.29 0.06

Flix Reserve
0.26 0.04

bc

bc

cd 1.00 0.13
a

ab

c 0.64 0.08
cd

2.06 0.48

0.24 0.18

ab

2.12 2.83

abcd 1.60 0.43

a 0.76 0.18
a 16.35 2.23
a

10.19 0.62

cd
1096.94 98.92
ab
336.81 15.76
cd
1099.29 141.89
a

443.54 49.62

ab 123.58 44.21
a30.48 16.83
abb
3.68 0.95
a

1.89 1.17

b
583.56 222.64
abc

243.39 95.44

Caspe2 Lake
0.32 0.05

2.31 0.42

bc1.48 0.68

1.89 0.51

cd

0.65 0.12
a 0.01 0.01

ab

1.40
1.99
a
0.83
a
17.87
a
11.17
a
553.13
c
485.08
ab
812.72
bc
578.98
ab
77.23
a
27.50
a 3.86
a 1.84
a171.79
abc
352.14
bcd

1.66
0.38
0.18
1.16
1.35
50.87
75.47
132.81
104.07
14.83
3.36
0.34
0.44
65.71
181.27

Fayn
0.12 0.04

e 0.69 0.12
c

0.87 0.03

0.64 0.14

0.95 0.11
bc
0.06 0.03

cd 0.55 0.05
bcd 0.14 0.18

b 1.70 1.27
cd

ab 1.82 1.58
a

2.07
a 0.65
a
18.08
a
11.06
b
900.17
d 610.03
abc
935.62
a 439.79
a
53.72
ab
34.13
abb
3.25
a
1.38
b 654.02
c
447.14

0.33
0.08
1.13
1.98
100.96
74.22
131.93
28.50
13.00
4.30
0.38
0.07
435.55
108.30

1.06 0.25

a
1.34 0.52
a
17.49 2.57
b

14.31
1149.64
c 497.43
cd1165.42
c
673.92
a
48.41
a
25.11
b 2.64
a 1.87
ab 411.33
abc
245.67

cd

1.30
79.14
28.35
216.38
80.79
9.71
4.75
0.35
0.62
105.27
29.56

Ionoregulation andosmoregulation
and osmoregulation
inDreissena polymorpha
p y
p
Watergainandionlossesare themainosmoticproblemsfor
freshwater animals
freshwateranimals.

Wesuspectedofosmoticregulationdisturbancesinthe
populationsatthemostanthropogenicimpactedsites.

Asallfreshwateranimalsdo,Dreissena
As all freshwater animals do, Dreissena regulatesitsblood
regulates its blood
osmoticpressuresatlevelshyperosmotictofreshwater.

of ion composition inthe

in the extracellular
Regulation ofioncomposition
mediaby ephithelial transport inthe gills.

Proteomicstudy
d

2D Electrophoresis

IMAGEANALYSIS

3626 spots
detected
STATISTICS
242 proteinsshowed
proteins showed
differencesinabundance
amongpopulations(p<0.05)

Cl
Cluster
analysis
l i

Caspe1

Mequinenza Caspe2

j
Sitjar

Fayon
y

PCA
PES=ProteinExpresionSignature

Mass Spectrometry and data base

search

79 proteinswere
identified

V
VtypeprotonATPasesubunitB
ATP
b i B

V
VtypeprotonATPasesubunitB
ATP
b i B

V
VtypeprotonATPasesubunitB
ATP
b i B

V
VtypeprotonATPasesubunitB
ATP
b i B

Summarizing
Inter-population differences were found in

Osmoregulatory performance
K content in gills and midgut gland
Gill NaK ATPase activity
Gill V-type proton ATPase protein abundance

Along with a unique protein expresion signature (PES)

in g
gills for each p
population.
p

High fenotype plasticity.