FLAVONOID

Cho bt cn tm vo trong bnh nn. Cho 30ml dung dch Ethanol 96%. un si. Lc qua bng gn. Chia lm 5 ng nghim, mi ng 2ml (1 ng lm ng chng kim tra khi tc dng vi NaOH, 4 ng tc dng vi th). 1. Phn ng nhm OH phenol: 2ml dch chit + vi git NaOH tng mu (so vi ng chng). 2ml dch chit + vi git FeCl3 xanh en 2ml dch chit + vi git Pb(CH3COO)2 vng ngh Trong c OH phenol 2. Phn ng vng C (vng - pyron) (c hiu): 2ml dch chit + bt Mg + HCl mu thanh long, mn (si bt kh H2) (Bt Mg cho bng u bt, cho HCl t t v khong 1ml)

Ghi trong bi thi:

Tm xem trong bt dc liu s c cha Flavonoid Dch chit sau khi thu c thc hin cc phn ng sau: 1. Dch chit + thuc th NaOH tng mu (hay khng tng) (+) hay (-) 2. Dch chit + thuc th FeCl3 xanh en (hay mu ) (+) hay (-) 3. Dch chit + thuc th Pb(CHCOO)2 vng ngh (hay mu ..) (+) hay (-) 4. Dch chit + thuc th Mg, HCl mu thanh long, mn (hay mu .) (+) hay (-) Kt lun: Bt s c cha Flavonoid (hay khng c cha Flavonoid).

ALKALOID
Cho bt cn tm vo trong bnh nn. Cho NaOH 10% vo (va m, cho t t v dng a thy tinh khuy u). 10 pht. Cho 30ml dung dch CH2Cl2 vo v un nh. Lc qua bnh lng cn (bng bng gn). Cho 15 ml dung dch H2SO4 long ri lc xoay vng (lc nh). B lp di, ly lp trn dch chit. Chia thnh 3 ng nghim (mi ng khong 2ml) 1. 2ml dch chit + thuc th Mayer vng 2. 2ml dch chit + thuc th Dragendorff cam 3. 2ml dch chit + thuc th Bouchardat nu (Phn ng ng thi 3 ng nghim, phi c v c i mu)
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n thi TH Dc liu

Ghi trong bi thi:

Tm xem trong bt dc liu s . cha Alkaloid. Dch chit sau khi thu c thc hin cc phn ng sau: 1. 2ml dch chit + thuc th Mayer vng (+) 2. 2ml dch chit + thuc th Dragendorff cam (+) 3. 2ml dch chit + thuc th Bouchardat (+) Kt lun: Bt s . c cha Alkaloid (hay khng c cha Alkaloid).

ANTRAGLYCOSID
1. Tm dng t do: Cho bt cn tm vo trong bnh nn. Cho 30ml dung dch CH2Cl2, lc u. Lc thng vo bnh lng cn. Cho 10ml dung dch NaOH 10% v, lc u, c lp trn c mu (+) C cha Antraglycosid dng t do. 2. Tm dng kt hp: Nu th nghim tm dng t do m tnh th ly b lm th nghim tm dng kt hp, b qua giai on ra b. Nu dng tnh th ta phi ra b: Cho b vo trong bnh nn. Cho 30ml dung dch CH2Cl2 vo, lc u v lc. Ta lm giai on ny 2 - 3 ln. Lm kh b: Cho b vo trong bnh nn v cho vo ni cch thy. Sau khi lm kh b, ta cho 30ml dung dch H2SO4 25%, lc u, un si nh ri lc, vo bnh lng cn. Cho 10ml dung dch CH2Cl2 vo v lc. Ly lp di khong 2-3ml cho vo ng nghim. Cho 3ml dung dch NaOH 10% vo ng nghim, lc mnh c mu hng, . c dng kt hp.

Ghi trong bi thi:

Tm xem trong bt dc liu s . cha Antraglycosid. Dch chit sau khi thu c thc hin cc phn ng sau: 1. Tm Antraglycosid dng t do: Dch chit + thuc th NaOH Lp trn c mu (+) Kt lun: c Antraglycosid dng t do a gio vin k tn. 2. Tm Antraglycosid dng kt hp: Dch chit + thuc th NaOH lp trn c mu hng () (+)
n thi TH Dc liu Trang 2

 Kt lun: c Antraglycosid dng kt hp. a gio vin k tn.

TANIN
Cho bt cn tm vo trong bnh nn. Cho 50ml H2O (nc si) vo, lc mnh, mang i lc lin (bng bng gn). Xp giy lc, lm t giy lc. Lc ln 2 bng giy lc trn (lc k dch chit trong). Chia thnh 4 ng nghim, mi ng khong 2-5ml. 1. Tm Tanin: Dch chit + thuc th Gelatin vng ng (+) Dch chit + thuc th FeCl3 xanh en (+) C Tanin (phi c ng thi 2 phn ng trn). 2. Tm Tanin Pyrocatechic PC (un ch cn chuyn sang mu hng da): 5ml dch chit + thuc th Stiasny (khng 20 git) + un nng vn gch (+) C cha Tanin PC 3. Tm Tanin Pyrogallic PG: Lc ng nghim phn ng trn (bng bng gn). Loi thuc th tha bng cch cho Na2CO3 vo si bt kh (cho tng git, lc u cho n khi ht si bt kh). Cho 1 - 3 git FeCl3 xanh en (+)

Ghi trong bi thi:

Tm xem trong bt dc liu s . cha Tanin. Dch chit sau khi thu c thc hin cc phn ng sau: 1. Tm Tanin: Dch chit + thuc th Gelatin vng ng (+) Dch chit + thuc th FeCl3 xanh en (+) Kt lun: bt c Tanin a Gio vin k tn. 2. Tm Tanin Pyrocatechic: Dch chit + thuc th Stiasny gch (+) Kt lun: c cha Tanin PC a Gio vin k tn. 3. Tm Tanin Pyrogallic: Dch chit + thuc th Na2CO3 + FeCl3 xanh en (+) Kt lun: c cha Tanin PG. a gio vin k tn.

n thi TH Dc liu

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