IA| |K| |KAI IK|EN KK&|N, IA| l, B 4 lI

THNH PHN HA H"C V TNH KHNG OXY HA C%A NGH( *EN

Curcuma zedoaria Berg. TR+NG , VI(T NAM
Tr.n Th1 Vi3t Hoa, Tr.n Th1 Ph56ng Th7o, V9 Th1 Thanh Tm
Tr!ng $%i h(c Bch khoa, $HQG-HCM
1. GI<I THI(U
Ngh* -en (Curcuma zedoaria Berg.) thu.c lo%i thn th/o, cao -1n 1,5m. Cy m(c hoang d%i
3 nhi4u n"i: b! su5i, ru.ng b6 hoang, mi4n niNgu7n g5c 3 Hymalaya, Srilanka, 8n $.,
Indonesia, Malaysia. ; Vi*t Nam, ngh* -en ->c tr7ng nhi4u 3 Bnh D"ng, $ L%t, Gia Lai
-? lm thu5c. C@ ngh* -en c hnh trB, di 2-5cm, -!ng knh 1-3cm. V6 c mu xm, phCn thDt
c mu trFng 3 lHp bn ngoi, mu tm nh%t 3 lHp trong, c mi th"m -Jc trng [1, 2]. Trong y
h(c cK truy4n, tL lu -!i ngh* -en ->c dng -? trD b*nh xanh xao, thi1u mu, tMng c!ng bi ti1t
mNt, tMng tr"ng lOc 5ng tiu ha, km Mn, nPm mn tnh -!ng ru.t, vim lot d% dy[1, 2, 4,
5].
NhQng ph"ng php trch ly tinh dCu, thnh phCn ha h(c, ho%t tnh khng oxy ha ->c
kh/o st trn hai lo%i nguyn li*u l c@ ngh* -en t"i v kh. NhQng k1t qu/ -%t ->c gp phCn
khSng -Dnh gi trD thi1t thOc c@a loi thOc vNt ny.
2. NGUYN LI(U V PH>-NG PHP TH?C NGHI(M
2.1. Chu@n b1 nguyn li3u
Cy ngh* -en tr7ng 3 $ L%t (Vi*t Nam), thu ho%ch vo thng 2, sau khi mua v4 ->c tch
ring cc phCn rT, thn, l, c@. RVa s%ch -Pt, lo%i b6 cc phCn h. Tch ring phCn c@, thn, l v
ph"i kh, cFt lt -? dT sPy, sPy nguyn li*u 3 nhi*t -. 50 -1n 60
0
C trong kho/ng 20 gi!. Sau -,
nguyn li*u ->c xay nh6 v xc -Dnh -. Wm. $. Wm c@ ngh* t"i l (76,38 0,16%); ngh* kh
l (10,47 0,08%). Cc ha chPt sV dBng: eter dCu h6a (60
0
90
0
C), CH
2
Cl
2
, EtOH 96% (Trung
Qu5c)
2.2. *iBu chC cc cao cEa cE ngh3 Fen:
500g b.t c@ ngh* -en lCn l>t ->c trch vHi dung mi eter dCu h6a, CH
2
Cl
2
, EtOH 96% bXng
h* th5ng trch Soxhlet. Cc dDch trch c@a cng lo%i dung mi ->c tNp h>p l%i v c quay chn
khng (50
0
C, 30mmHg) -? lo%i dung mi -1n khi thu ->c cao rFn c kh5i l>ng khng -Ki.
2.3. Kh7o st FiBu ki3n ch5ng cGt tinh d.u cE ngh3 Fen
Tinh dCu ->c chng cPt tL c@ ngh* bXng h* th5ng chng cPt li cu5n h"i nHc c h7i lu.
Chng ti kh/o st hm l>ng tinh dCu thu ->c trn hai lo%i nguyn li*u ngh* t"i v ngh* kh
theo 2 ph"ng php: ph"ng php gia nhi*t -5t nng thng th!ng (PPT) v ph"ng php gia
nhi*t bXng vi sng (PPVS) vHi sO thay -Ki t l# L/R (th' tch n)*c (ml) : kh-i l)0ng nguyn li#u
(g)): 1:3, 1:4, 1:5, 1:6 (-5i vHi ngh* t"i) v 1:11, 1:15, 1:19, 1:23 (-5i vHi ngh* kh) theo th1i
gian ch)ng c3t.
$7ng th!i, nhXm -nh gi s" b. chPt l>ng tinh dCu, chng ti ti1n hnh kh/o st cc tnh
chPt ha l c@a chng nh: mu, mi, vD, tY tr(ng, chi1t suPt, gc quay cOc, ch[ s5 acid, ch[ s5 x
phng, ch[ s5 ester, ch[ s5 iod.
2.4. Kh7o st thnh ph.n ha hHc cEa tinh d.u theo ph56ng php GC/MS
Thnh phCn ha h(c c@a tinh dCu v cao ete dCu h6a (phCn khng x phng ha) ->c -Dnh
danh v -Dnh l>ng bXng ph"ng php sFc k ghp kh5i phK (GC/MS) t%i Trung tm Phn tch v
ki?m nghi*m thu5c th y Trung "ng II.
Cc m]u ->c phn tch trn my GC: FISONS GC 8000 series, -Cu d kh5i phK: FISONS
Intruments MD 800.
t|ttt & Itt|1|1) trt|1Ntl, f1| l, N1.4 lI

i!u ki$n s&c k [3, 6, 7, 14]
C.t: DB5MS, chi4u di 30m, -!ng knh 0,25mm; = 0,25 m.
Ch"ng trnh nhi*t -.:
90
0
C (1 pht) 130
o
C 180
o
C 270
o
C (5 pht)

Nhi*t -. bu7ng ha h"i 250
o
C, th? tch tim 1l, chia dng 1%. Kh mang l Helium vHi p
suPt kh mang: P
He
= 18 psi.
i!u ki$n kh*i ph,
Ch"ng trnh MS: Theo ch1 -. Full Scan FC 43 (29 500 a.m.u). BFn ph ion
:EI
+
. Electron energy: 70eV. C!ng -. dng Emission:150A. Nhi*t -. b. ngu7n: 200
0
C. Nhi*t
-. b. phNn giao ti1p (Interface): 250
0
C. Sau -, cc kh5i phK m]u ->c so snh vHi kh5i phK
chuWn trong th vi*n NIST Ver 2.0a.
2.5. Kh7o st tnh khng oxy ha cEa Ngh3 Fen:
Ho%t tnh khng oxy ha c@a tinh dCu v cc dDch trch Ngh* -en ->c kh/o st bXng ph"ng
php Ferric Thiocyanat (FTC) c@a Mitsuda, Osawa (1967). Sau -, ph"ng php ny ->c c/i
ti1n b3i Kikuzaki v Nakatani (1993) [8, 9, 10, 11].
Cc m]u cCn kh/o st lCn l>t ->c pha 3 cc n7ng -. khc nhau, tL 100 400mg trong 8ml
c7n tuy*t -5i. Sau -, thm vo m^i dung dDch 4ml acid linoleic 2,51% trong EtOH v 8ml dung
dDch -*m phosphat (pH 7,0). Cu5i cng, cc dung dDch ->c -Ny kn, lFc 3 nhi*t -. phng v -?
trong t5i.
Sau m^i kho/ng th!i gian nhPt -Dnh (24 48 gi!), lPy ra 0,1 ml dung dDch. LCn l>t cho vo
9,7ml dung dDch EtOH 75% (theo th? tch); 0,1ml dung dDch NH
4
SCN 30% v 0,1ml dung dDch
FeCl
2
20mM (trong HCl 3,5%). Chnh xc 3 pht sau khi cho FeCl
2
vo dung dDch ph/n _ng, ti1n
hnh -o -. hPp thu c@a dung dDch t%i bHc sng 500nm. Th nghi*m k1t thc khi m]u trFng -%t
-. hPp thu cao nhPt t%i bHc sng ny.
% khng oxy ha = 100 x
A
A - A
trang mau 500nm,
hoa oxy ng chat kha co mau 500nm, trang mau 500nm,
%
3. KJT QUL V BN LUMN
3.1. *iBu ki3n ch5ng cGt tNi 5u cho ngh3 kh v ngh3 t56i:
+ Tinh dCu ngh* t"i theo ph"ng php chng cPt cK -i?n: NTT
+ Tinh dCu ngh* t"i theo ph"ng php chng cPt c h^ tr> vi sng: NTVS
+ Tinh dCu ngh* kh theo ph"ng php chng cPt cK -i?n: NKT
+ Tinh dCu ngh* kh theo ph"ng php chng cPt c h^ tr> vi sng: NKVS
+ Cao eter dCu h6a trch ly bXng Soxhlet: cao EDH
B7ng 1.Hm l>ng tinh dCu v th!i gian chng cPt giQa cc ph"ng php dOa
trn cc -i?m t5i u.
ChO tiu so snh NTT NTVS NKT NKVS Cao EDH
Hm l>ng tinh
dCu* (%)
6,68 0,05 6,10 0,07 8,66 0,14 7,76 0,06 4,60 0,06
Th!i gian (pht) 170 140 110 90 90
5
0
C/pht
2
o
C/pht
15
o
C/pht
IA| |K| |KAI IK|EN KK&|N, IA| l, B 4 lI

TY l* (R:L) t5i u 1:4 1:4 1:15 1:15 1:10
(*): Tnh trn l)0ng nguyn li#u kh tuy#t 5-i
PPT cho hm l>ng tinh dCu cao h"n PPVS. Tuy nhin, PPVS tiu t5n t th!i gian h"n PPT
(kho/ng 9-12,5%). Hm l>ng tinh dCu thu ->c bXng PPVS c th? cao hay thPp h"n PPT l ty
thu.c vo thnh phCn ha h(c c@a d>c li*u.
Khi chng cPt, d l ph"ng php no, ngh* kh lun lun cho l>ng tinh dCu cao v tiu t5n
t th!i gian chng cPt h"n ngh* t"i v ngh* t"i c hm l>ng Wm cao (> 76%).
Hm l>ng tinh dCu cao nhPt 3 tY l* 1:4 -5i vHi ngh* t"i v tY l* 1:15 -5i vHi ngh* kh. VHi
l>ng nHc qu t (t[ l* 1:1; 1:2), 3 nhi*t -. si c@a nHc nguyn li*u s` dT bD vn cBc, chy kht
lm bi1n tnh chPt l>ng tinh dCu. ; tY l* R/L cao h"n, khi l>ng nHc chng qu nhi4u, nhi*t -.
chng cao v th!i gian ko di s` lm cho m.t s5 thnh phCn c@a tinh dCu bD th@y phn, bD oxy
ho khi c mJt c@a enzym, hoJc cc thnh phCn phn cOc trong tinh dCu bD ho tan trong nHc,
lm gi/m l>ng tinh dCu thu ->c.
3.2. Tnh chGt ha l cEa tinh d.u Ngh3 Fen:
B7ng 2.Cc tnh chPt ha l c@a tinh dCu.
ChO sN ha l NTT NTVS NKT NKVS
Mu Vng sNm Vng t"i Xanh lBc -Nm Xanh lBc
Mi RPt n7ng RPt n7ng N7ng N7ng
Tr%ng thi Trong, snh Trong, rPt snh Trong, h"i snh Trong, rPt snh
VD $Fng, cay $Fng, cay $Fng, cay $Fng, cay
TY tr(ng,
30
30
d 0,986 0,005 0,992 0,003 0,981 0,009 0,993 0,009
Chi1t xuPt,
30
D
n 1,521 0,013 1,523 0,018 1,520 0,012 1,524 0,015
$. quay cOc,
] [
30
D

9,26 0,34 32,01 0,25 9,38 0,23 34,08 0,35
$. ho tan tinh
dCu trong c7n
90
o

(ml/ml)
1:40 1:25 1:35 1:23
Ch[ s5 acid 0,52 0,01 0,78 0,02 0,72 0,01 0,82 0,02
Ch[ s5 ester 12,9 0,8 21,8 0,2 26,0 0,4 31,6 1,2
Ch[ s5 x
phng
13,4 0,8 22,6 0,2 26,7 0,4 32,4 1,2
Ch[ s5 iod 194 4 146 2 198 2 184 1

Cc ch[ s5 ha l c@a tinh dCu Ngh* -en chng cPt theo cc ph"ng php khc nhau khng
hon ton gi5ng nhau. $i4u ny l ph h>p v k1t qu/ phn tch GC/MS cho thPy hm l>ng v
thnh phCn ho h(c trong tinh dCu Ngh* -en c sO khc nhau rPt nhi4u giQa cc ph"ng php
chng cPt.
3.3. Thnh ph.n ha hHc cEa tinh d.u Ngh3 Fen
t|ttt & Itt|1|1) trt|1Ntl, f1| l, N1.4 lI


Hnh 1. K1t qu/ GC c@a tinh dCu c@ ngh* t"i tL chng cPt cK -i?n.


Hnh 2.K1t qu/ GC c@a tinh dCu c@ ngh* t"i tL chng cPt cK -i?n c h^ tr> vi sng

Hnh 3.K1t qu/ GC c@a tinh dCu c@ ngh* kh tL chng cPt cK -i?n

Hnh 4. K1t qu/ GC c@a tinh dCu c@ ngh* kh tL chng cPt cK -i?n c h^ tr> vi sng
IA| |K| |KAI IK|EN KK&|N, IA| l, B 4 lI


Hnh 5.K1t qu/ GC c@a cao ete dCu h6a c@ ngh* -en (phCn khng x phng ha)
t|ttt & Itt|1|1) trt|1Ntl, f1| l, N1.4 lI

B7ng 3.K1t qu/ MS c@a m.t s5 thnh phCn chnh trong cc lo%i tinh dCu v cao ete dCu h6a (phCn
khng x phng).
Thnh phCn
ThQi gian
l5u
KhNi phS
-Elemen
11,940
(mainlib) -Elemene
20 40 60 80 100 120 140 160 180 200
0
50
100
29
41
53
67
79
93
107
121
133
147
161
175
189
204

Curzeren
14,145
(mainlib) Benzofuran, 6-ethenyl-4,5,6,7-tetrahydro-3,6-dimethyl-5-isopropenyl-, trans-
10 30 50 70 90 110 130 150 170 190 210 230
0
50
100
15
27
39
53 65
79
91
108
115
133
148
159
173
187
201 216
O

Germacron
20,742
(mainlib) 3,7-Cyclodecadien-1-one, 3,7-dimethyl-10-(1-methylethylidene)-, (E,E)-
20 40 60 80 100 120 140 160 180 200 220
0
50
100
27
41
53
67
82
91
107
121
135
147
161
175
190
203
218
O

6-Tert-
butyl-4-
metyl-
coumarin
30,871
(mainlib) 6-tert-Butyl-4-methylcoumarin
10 30 50 70 90 110 130 150 170 190 210 230
0
50
100
15 27
39
51
63
77
91
115
128
145 161
173
201
216
O O


B7ng 4.Thnh phCn tinh dCu c@ ngh* -en phn tch bXng GC/MS
STT Thnh ph.n Thnh ph.n ph.n trTm (%)
IA| |K| |KAI IK|EN KK&|N, IA| l, B 4 lI

NTT NTVS NKT NKVS Cao KXP
1. Camphor 3,810,11 4,600,21 2,490,22 2,880,12 0,230,03
2. Isoborneol 0,890,11 1,640,10 0,390,02 0,730,12 0,100,01
3. Borneol 0,270,02 0,650,01 0,130,02 0,270,02 -
4. -Elemen 0,940,02 0,300,05 0,830,02 0,240,02 0,480,02
5. -Elemen 1,750,02 0,580,01 1,820,01 0,590,02 2,460,02
6. -Elemen 18,791,45 15,191,13 18,491,22 14,181,37 15,231,25
7. -Humulen 1,540,10 0,720,10 1,960,22 1,720,13 0,900,23
8. Germacren D 1,470,21 0,600,11 1,920,23 1,120,21 1,430,02
9. -Cubeben 0,450,02 0,310,02 0,710,02 0,690,03 -
10. Curzeren 15,222,11 15,792,02 14,281,99 16,672,06 34,272,02
11. -Guaien 0,770,02 0,230,01 0,750,01 0,670,02 -
12. Guaian 0,460,02 0,510,04 0,540,03 0,850,03 -
13.
5H-Inden-5-on-
1,2,3,6,7,7a-
hexahydro-7a-
metyl
- - - - 1,170,05
14.
Cycloisolongif
olen,8,9-
dihydro-9-
formyl
3,580,05

4,490,07

3,170,04

3,900,04

-
15.
2-Cyclohexen-
1-on, 4-etylnyl-
4-hydroxy-
3,5,5-trimetyl
- - - -
1,840,07

16. Spathulenol 3,140,06 4,180,06 4,580,07 5,390,09 0,780,10
17. Germacron 23,942,21 24,282,19 22,532,18 23,102,23 3,330,04
18.
Isoaromadendr
en epoxid
0,630,02 0,720,02 1,100,04 1,260,05 -
19.
Acid acetic, 3-
hydroxy-6-
isopropenyl-
4,8a-dimetyl-
1,2,3,5,6,7,8,8a
-
octahydronapht
alen
- - - -
4,460,07

20.
Aristolen
epoxid
- - - - 0,780,03
21.
4,4-Dimetyl-3-
(3-metylbut -2-
enyliden)
octan-2,7-dion
- - - - 1,850,04
22.
9-Octadecynoid
acid, metyl este
2,330,04 2,780,03 2,710,04 3,460,06 -
23. Cedren-13-ol - - - - 1,790,13
24.
Spiro[4,5]dec-
6-en-on,1,7-
- - - - 0,960,20
t|ttt & Itt|1|1) trt|1Ntl, f1| l, N1.4 lI

dimetyl-4-(1-
metyletyl)
25. Leden oxid 1,790,06 3,090,07 2,440,03 3,430,05 -
26.
Hexadeca-
2,6,10,14-
tetraen-1-ol
- - - - 0,980,13
27.
6-Tert-butyl-4-
metylcoumarin
- - - - 6,720,31
28.
Cyclocostunoli
d
0,270,01 1,950,01 0,800,02 1,570,02 -
29.
Acid acetic,7-
isopropenyl-
1,4a-dimetyl-3-
oxo-
2,3,4,4a,5,6,7,8
-
octahydronapht
alen
- - - - 1,090,02
30.
1-Eicosanol
(C
20
H
41
OH)
- - - - 0,670,02
31.
13-Tetradecen-
1-ol acetat
- - - - 0,670,03
32.
Tertratriaconta
n (C
34
H
70
)
- - - - 0,700,04
33.
1-
Hentetracontan
ol (C
41
H
83
OH)
- - - - 0,450,08
34.
Pentratriaconta
n (C
35
H
72
)
- - - - 0,270,02
TSng cVng 82,042,21 82,612,34 81,642,22 82,722,31 83,612,19
Thnh phCn chnh c@a tinh dCu c@ ngh* -en trch ly theo PPT hay PPVS l -Elemen (14,18
1,37% FCn 18,79 1,45%), Curzeren (14,28 1,99% FCn 16,67 2,06%), Germacron (22,53
2,18% FCn 24,28 2,19%). $7ng th!i, hm l>ng cc thnh phCn ny trong tinh dCu ngh* -en
t"i cao h"n trong tinh dCu ngh* -en kh.
Cc thnh phCn km phn cOc (-Elemen, -Elemen , -Elemen, -Humulen, Germacren D,
-Cubeben, -Guaien) trong tinh dCu ngh* chng bXng PPVS thPp h"n so vHi PPT. Ng>c l%i,
nhQng thnh phCn phn cOc h"n do c ch_a Oxi nh Camphor; Isoborneol; Borneol; Curzeren;
Guaian; Cycloisolongifolen,8,9-dihydro-9-formyl; Spathulenol; Germacron; Isoaromadendren
epoxid; 9-Octadecynoid acid, methyl ester, Leden oxid; Cyclocostunolid l%i chi1m hm l>ng cao
h"n. V vi sng u tin tc dBng trn nhQng thnh phCn phn cOc, gip nhQng thnh phCn ny
nhanh tMng nhi*t -., thot ra kh6i t1 bo v bD li cu5n theo h"i nHc dT h"n.
Thnh phCn chnh trong cao eter dCu ho/ l: -Elemen (15,23 1,25%), Curzeren (34,27
2,02%), 6-Tert-butyl-1-4-metylcoumarin (6,72 0,31%), cn Germacron ch[ chi1m 3,33
0,04%.
DDch trch eter dCu h6a (cao EDH) ch_a rPt nhi4u h>p chPt h"n so v" tinh dCu thu ->c tL
chng cPt. V cc chPt khng v km phn cOc ho tan t5t trong dung mi eter dCu h6a. Cc h>p
chPt phn cOc nh: 9-Octadecynoid acid, methyl ester, -Guaien; Guaian;
Cycloisolongifolen,8,9-dihydro-9-formyl; Isoaromadendren epoxid; Borneol; -Cubeben l%i
khng hi*n di*n trong dDch trch eter dCu h6a v chng khng tan trong dung mi khng phn cOc.
IA| |K| |KAI IK|EN KK&|N, IA| l, B 4 lI

3.4. KCt qu7 khng oxy ha cEa Ngh3 Fen
0
20
40
60
80
100
A B C D E F
%

k
h
a
n
g

o
x
y

h
o
a
5.0 (mg/ml) 10.0(mg/ml)
15.0 (mg/ml) 20.0(mg/ml)

Hnh 6.Kh/ nMng khng oxy ha c@a ngh* -en theo n7ng -. (Ph"ng php FTC)
40
50
60
70
80
90
100
5.0 10.0 15.0 20.0
No ng do (mg/ml)
%

k
h
a
n
g

o
x
y

h
o
a
Cao eter dau hoa cu Nghe den Cao diclometan cu Nghe den
Cao con cu Nghe den Tinh dau Nghe kho thuong
Tinh dau Nghe kho vi song Acid Ascobic

Hnh 7.Kh/ nMng khng oxy ha c@a ngh* -en theo n7ng -. (Ph"ng php FTC)
VHi:
A: Cao eter dCu h6a c@a c@ Ngh* -en. D: Tinh dCu Ngh* -en kh (PPT)
B: Cao CH
2
Cl
2
c@a c@ Ngh* -en E: Tinh dCu Ngh* -en kh (PPVS)
C: Cao c7n c@a c@ Ngh* -en F: Acid ascorbic (Vitamin C)
K1t qu/ kh/o st cho thPy m_c -. khng oxy ha c@a cc m]u thV trong kho/ng n7ng -. 5-
20mg/ml ->c sFp x1p nh sau:
Vitamin C (70,5 1,1% - 94,1 1,5%) > cao eter c@ (61,4 0,8% - 84,5 1,2%) > cao
CH
2
Cl
2
c@ (57,2 2,1% - 81,8 1,8%) > Tinh dCu NKVS (47,4 0,9% -77,8 0,7%) > cao c7n
c@ (45,3 1,3% - 75,2 1,7%) tinh dCu NKT (45,8 0,5% - 74,8 1,1%).
4. KJT LUMN
Qua qu trnh kh/o st, c th? rt ra ->c nhQng k1t luNn sau:
; PPT, -5i vHi ngh* t"i, hm l>ng tinh dCu (tnh trn l)0ng nguyn li#u kh tuy#t 5-i) l
6,68 0,05% trong th!i gian chng cPt 110 pht. $5i vHi ngh* kh, hm l>ng tinh dCu cao h"n
(-%t 8,66 0,14%) trong 170 pht.
; PPVS, hm l>ng tinh dCu -%t ->c 6,10 0,07% trong 90 pht (-5i vHi ngh* t"i) v
7,76 0,06% trong 140 pht (-5i vHi ngh* kh).
t|ttt & Itt|1|1) trt|1Ntl, f1| l, N1.4 lI

PPVS tuy cho th!i gian chng cPt ngFn h"n nhng l%i lm gi/m hm l>ng tinh dCu. $7ng
th!i, vHi chi ph cao, PPVS khng -%t hi*u qu/ t5t trong vi*c ly trch tinh dCu tL c@ Ngh* -en.
Thnh phCn chnh c@a tinh dCu c@ Ngh* -en trch ly theo PPT hay PPVS l -Elemen (14,18
1,37% FCn 18,79 1,45%), Curzeren (14,28 1,99% FCn 16,67 2,06%), Germacron
(22,53 2,18% FCn 24,28 2,19%). $7ng th!i, hm l>ng cc thnh phCn ny trong tinh dCu
ngh* -en t"i cao h"n trong tinh dCu ngh* -en kh. Trong khi -, Furanogermenon l thnh phCn
chnh trong tinh dCu tL ngh* -en Trung Qu5c, Curcumenol l thnh phCn chnh c@a tinh dCu ngh*
$i Loan cn Dehydrocurdion l h>p chPt ch@ y1u c@a tinh dCu tL ngh* NhNt B/n [2, 12, 13, 15].
Thnh phCn chnh trong cao eter dCu ho/ l: -Elemen (15,23 1,25%), Cuzeren (34,27
2,02 %), 6-Tert-butyl-1-4-metylcoumarin (6,72 0,31%), cn Germacron ch[ chi1m 3,33
0,04%.
Thnh phCn tinh dCu thu ->c tL cc ph"ng php khc nhau th khc nhau rPt nhi4u. V vNy,
khi _ng dBng trong thOc t1 cCn xem xt -i4u ki*n cB th? -? p dBng ph"ng php ph h>p.
K1t qu/ kh/o st tnh khng oxy ha cho thPy tinh dCu Ngh* -en c m_c -. khng oxy ha
t"ng -5i cao 3 n7ng -. 20mg/ml (74,8 1,1% - 77,8 0,7%). Cao eter dCu h6a c kh/ nMng
khng oxy ha cao nhPt (61,4 0,8% - 84,5 1,2%) 3 n7ng -. tL 5,0-20,0mg/ml. $i4u ny
ch_ng t6 cc h>p chPt c ho%t tnh sinh h(c m%nh tNp trung nhi4u 3 lo%i cao ny.
K1t qu/ nghin c_u bHc -Cu -%t ->c nhXm gp phCn -Dnh hHng cho nhQng nghin c_u
ti1p theo v4 thnh phCn v tc dBng d>c l (tnh khng nPm, khng vim, khng ung th) c@a
tinh dCu c@ ngh* -en Curcuma zedoaria 3 Vi*t Nam.
TI LI(U THAM KHLO
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IA| |K| |KAI IK|EN KK&|N, IA| l, B 4 lI

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