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Student Instruction Sheet

Challenge
See real-time evidence germinating seeds are engaged in cellular respiration.

Equipment and Materials

computer with USB port PASPORT USB interface PASPORT CO2 Gas Sensor DataStudio software Erlenmeyer flask, 250-mL magnifying glass

paper towel, 1 sheet beans or pea seeds (40) water, 100.0 mL Student Instruction Sheet
Student Response Sheet

Safety Precautions
Keep water away from electrical outlets, the computer, the keyboard, and the PASPORT equipment! Follow standard laboratory safety procedures. Do not immerse the end of the CO2 Gas Sensor in water.

Background
When seeds become moist, and water penetrates into them, enzymes begin the process of cellular respiration to provide the new plant with the fuel that it needs for the building of new material. Cellular respiration is the process of oxidizing food molecules such as sugar into carbon dioxide and water, producing energy-rich molecules that fuel plant growth. Therefore, an increasing carbon dioxide (CO2) concentration inside the flask with the germinating seeds is evidence that cellular respiration is occurring as the seeds germinate.

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Predict
Before beginning the eLab, complete the prediction and vocabulary portions of the Student Response Sheet.

Explore
Computer Setup
1. Plug the USB interface into the computers USB port. 2. Plug the CO2 Gas Sensor into the USB interface. This will automatically launch the PASPortal window.
To computer

3. Choose the appropriate DataStudio configuration file entitled 04 Cellular Respiration CF.ds and proceed with the following instructions.
Note:

Configuration files automatically launch the appropriate display(s), sampling rate(s), etc.

Equipment Setup
1. Fold a paper towel in half along its length, and then fold it in half along its width. Now the paper towel should have 4 layers and be approximately square in shape. 2. Place about 15-20 bean or pea seeds on the towel towards one edge of the towel. 3. Fold about 1 inch of that edge of the towel over the beans.

4. With the folded end on an outside edge, roll the towel into a cylinder, trapping the beans inside. The cylinder will have one open end and one nearly closed end.

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5. Slide the paper towel cylinder into the Erlenmeyer flask with the closed end towards the bottom.
Note:

It is okay if a few beans (or seeds, if using pea seeds) fall out during this procedure.

6. Slide the end of the CO2 Gas Sensor inside the open end of the paper towel. Firmly plug the opening of the flask with the rubber stopper of the CO2 Gas Sensor.

Record Data
1. In DataStudio on the Experiment menu, click Monitor Data. Monitor the data until the levels of CO2 gas stabilizes.
Note:

USB Link

CO

The system will require a few minutes to equilibrate. You should not need to monitor the data longer than 5 minutes. Avoid bumping the equipment, because bumping or jarring will cause the CO2 Gas Sensor to behave erratically.

Gas Sensor

paper towel cylinder containing bean seeds end of sensor is inside paper cylinder 250-mL Erlenmeyer flask

2. When the CO2 gas readings stabilize, click the Stop ( ) button to stop monitoring. 3. Click the Start button ( recording data.
Note:

) to start

DataStudio has been set to record data at a rate of 1 sample every 15 seconds.

4. Record data for 20 minutes. Click the Stop button.

Result:

This data will be stored in Run 1. Make a note in your data table on your Student Response Sheet that Run 1 is data collected from dormant bean seeds.

5. Carefully remove the CO2 Gas Sensor from the flask and add 100.0 mL of water to the flask.

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6. Carefully insert the CO2 Gas Sensor into the flask as before, sealing the top of the flask with the rubber stopper. 7. Click the Start button to begin recording data. 8. Leave this setup alone and allow data recording to proceed for 24 hours. 9. After 24 hours, Stop recording data.
Result:

This data will be stored in Run 2. Make a note in your data table on your Student Response Sheet that Run 2 is data collected from germinating seeds.

Analyze
1. Carefully remove the CO2 Gas Sensor from the flask and return it to its storage bag. 2. Pull the paper towel/seed package out of the flask. 3. Use the magnifying glass to observe the seeds. 4. Record your observations on the Student Response Sheet. 5. Record the concentration of CO2 gas at the beginning and end of the data Run #1.
Hint:

Expand the scale and move the position of the x- and yaxes until you can easily see all the data. To rescale an axis of the graph, hover the cursor over the axis until the rescale symbol ( ) appears, and click and drag the axis to rescale it. To move the origin of the axis, hover the cursor over the axis until the hand symbol ( ) appears, and then click and drag the axis to move the desired range into view.

6. Record the concentration of CO2 gas at the beginning and end of the data Run #2. 7. Save your DataStudio file (on the File menu, click Save Activity As...) to the location specified by your teacher. 8. Answer all questions on the Student Response Sheet. 9. Follow your teachers instructions regarding cleaning up your work space.

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Student Response Sheet

Name: ______________________________ Date:_______________________________

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Vocabulary
Use available resources to find the definitions of the following terms: adenosine diphosphate (ADP):______________________________________ ___________________________________________________________ adenosine triphosphate (ATP): _____________________________________ ___________________________________________________________ carbon dioxide (CO2): ___________________________________________ ___________________________________________________________ cellular respiration: _____________________________________________ ___________________________________________________________ germination: __________________________________________________ ___________________________________________________________

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Predict
What do you think will happen to the seed after 24 hours of being in a moist environment? What do you think will happen to the level of CO2 inside the flask? ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ ____________________________________________________________

Data
Data Run Condition of seeds Appearance of seeds Concentration of CO2 (ppm) Before Run
1 2

After Run

Draw a sketch of your graph of CO2 concentration. Label the x- and y- axes, and show the portion that represents time when seeds were dormant and time when seeds were germinating.

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Analyze
1. Describe your graph. ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ 2. Did your observation of the levels of CO2 after you added water to the flask match your expectations? Why or why not? ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ 3. In your own words, explain why the levels of CO2 changed the way they did after you added water to the flask. ____________________________________________________________ ____________________________________________________________ ____________________________________________________________

Synthesize
1. Find an expression (formula) in your textbook that summarizes the net molecular changes occurring in cellular respiration, and write the expression here. ____________________________________________________________ ____________________________________________________________ ____________________________________________________________

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2. Judging from this expression, what gaseous molecule would you expect to be produced during cellular respiration? ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ 3. If cellular respiration occurs during germination, what would you expect would happen to the levels of CO2 inside the flask after you added water? ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ 4. Was your prediction correct? Why or why not? ____________________________________________________________ ____________________________________________________________ ____________________________________________________________

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