Mi trng nui cy nm mc (mi trng Czapek): l mi trng tng hp Chun b: Mi trng Czapek 3 a petri x 25ml 4 phng m 4 a petri cha:

cha: giy lc, 2 que tre, lame, 2 lamelle

Thnh phn mi trng Czapek Saccharose: 3g NaNO3:0. 3g K2HPO4: 0.1g KCl: 0.05g MgSO4.7H2O: 0.05g FeSO4.7H2O: 0.001g Agar: 3g Nc ct: 100ml pH= 6, kh trng 1atm/30 pht Cch pha ch mi trng nui cy: Cn chnh xc v cho cc thnh phn ca mi trng vo trong cc cha sn 1 t nc. Khuy cho tan ht v them lng nc theo yu cu. un ln cho tan hon ton. ngui v lng dung dch va un. Hp kh trng chai mi trng. Phn phi mi trng vo cc dng c v trng.

Phng php cy: phng m Thng qua mu sc ca mc c th nhn din. Mc c mu trng: Mucor hoc Rhizopus Mc c mu en: Aspergillus niger Mc c mu xanh lc: Penicillum italicum Chun b a mi trng Czapek agar Chun b 1 a petri v trng cha cc mnh giy lc, 2 thanh ua tre v 1 lame. S dng 1 con dao v trng ct mt khi agar vi kch c va t ming lamelle.

Nhc khi thch ny ra khi a mi trng v chuyn sang a ni cy trn lame chun b sn. Dng dy cy hnh thc th ly 1 t sinh khi nm mc v cy vo 4 mt bn ca khi thch. Sau y lamelle ln trn khi thch va mi cy nm mc. Nh nc ct v trng cho t ton b giy thm trong a. y np a petri v a nhit phng cho n khi s tng trng v hnh thnh bo t xy ra. Ly lamelle ra khi khi thch. Nh 1 git Methylene blue ln lame sch v t lamelle ln Quan st di knh hin vi.

Mi trng nui cy nm men (mi trng Hansen): l mi trng bn tng hp Chun b: Mi trng Hansen Nc mui sinh l a petri: 16 a

Thnh phn mi trng Hansen: Glucose hoc Maltose: 50g Pepton: 10g KH2PO4: 3g MgSO4.7H2O: 2 5g Agar: 20g Nc ct: 1000ml Cch pha ch mi trng nui cy: Cn chnh xc v cho cc thnh phn ca mi trng vo trong cc cha sn 1 lt nc. Khuy cho tan ht v thm lng nc theo yu cu. un ln cho tan hon ton. ngui v lng dung dch va un. Hp kh trng chai mi trng. Phn phi mi trng vo cc dng c v trng.

Phng php cy : cy trang C th phn lp nm men d dng t cc mi trng nh: o B mt tri cy hoc dch p mt s tri cy nh to, l, nho, du.

Trong ru np, trong cc bnh men ru, bia, nc ma vi hm, ht kefir Mi trng phn lp: Hansen agar Cn 10g bnh men cm ru cho vo erlen v trng, b sung 90ml nc ct v trng v ng nht => nng pha long l 10-1. Sau tin hnh pha long thnh cc nng 10-2 10-3 10-4. mi nng pha long, tin hnh ht 0.1ml cho vo a mi trng Hansen agar v s dng que trang trang u dch trn mi trng Hansen. nhit 300C trong 24 - 48h. o