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,

-
-

Escherichia coli
Salmonella
Pseudomonas
aeruginosa
Staphylococcus
aureus

Enterobacteriaceae

Escherichia coli
Salmonella
Pseudomonas
aeruginosa
Staphylococcus
aureus
Clostridia


Gr(-)
Escherichia coli
Salmonella
Pseudomonas
aeruginosa
Staphylococcus
aureus
Clostridia
Candida albicans

Escherichia coli
Gr(-), ,


, 20-50
G


,

,

Salmonella
Gr(-), ,



,
,
, ,
, ,
,
, ,

Pseudomonas aeruginosa
Gr(-), , 1-2


-
, , ,


, (
),

Staphylococcus aureus

Gr(+), ,



(20% )
,

, , ,
,
(, ),
, , ,
, ,

-

Clostridium
Gr(+), -

(closter- ),



- 2S, ,
N2,
, ,
,

(<100-130)
, ,

, , -

Candida albicans



( ) (
80%),

.
(HIV-, ,
)


(, ),


, MO
American Type Culture Collection
NCIMB National Collection of Industrial and Marine Bacteria Ltd
CIP
Collection de Bacteries de lInstitut Pasteur
NCPF National Collection of Pathogenic Fungi

Staphylococcus aureus ATCC 6538


Bacillus subtilis ATCC 6633
Pseudomonas aeruginosa ATCC 9027
Clostridium sporogenes ATCC 19404
Candida albicans ATCC 16404
Salmonella ATCC
Escherichia coli ATCC


,

,


. -70, - t,

,

,

;

2

,
,
-, .

:
- t
- ()



/ / .
ATCC, CIP, NCIMB, NCPF.
.

.
/.
-,
,

,

5
.




70 %


(),


.

.


.

,



30 tC


,


>1,5
(127) 45-60 .


()
Nutrien agar ,
.

2-8 6 .

(Nutrie agar).
1
.

.
(working stock)
V
V .




CCLS (Clinical and Laboratory Standards Institute)
:
-50 -70 1
- -70

2-8 1 2


(American Type Culture Collection)
:
-80

-20
(- 1 )
2 8
- 2 8 - 1


30-

. 3 000 .

.
1911
.
,

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1.
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. , .
Pharmaceutical Microbiology Forum Newsletter vol.12(3)
US Pharmacopeia. Antimicrobial Effectiveness Testing. In: US
Pharmacopeia 26th rev. Rockville, MD: US Pharmacopeia; 2003,
2nd supplement (51)
US Pharmacopeia. Microbiological Good Laboratory Practices,
(1117). Pharmacopeial Forum 29(3): Pharmacopeial Reviews,
2003
NCCLS. Performance standards for antimicrobial disk susceptibility
tests. 8th ed. Wayne, PA: NCCLS; M2-A8, 2003
NCCLS. Reference method for broth dilution antifungal
susceptibility tasting of yeasts; approved standard. Wayne, PA:
NCCLS; M27-A, 1997
ATTC. Reference Strains: How many passages are too many?
Technical Bulletin 6
T. Uzunova-Doneva, T. Donev Anabiosis and conservation of
microorganisms, Journal of Culture Collections, vol.4, 2004-2005,
pp.17-28

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