B CNG THNG TRNG I HC CNG NGHIP THC PHM TP.

HCM KHOA CNG NGH THC PHM

Th nghim decarboxylase
GVHD: Phan Th Kim Lin Nhm: 2

NI DUNG
1. Nguyn tc

2. C s sinh ha
3. Phng php tin hnh

Nguyn tc: S dng pht hin vi khun sinh ezyme decarboxylase Enzyme decarboxylase xc tc phn ct nhm carboxyl ca acid amin trong iu kin k kh

iu kin to enzyme decarboxylase:

o Mi trng c tnh axit o Cha cht cm ng c bit( acid amin)

C s sinh ha:
(H2N)aR(COOH)b (amino acid)
e.decarborxylase

R-CH2-NH2(amine)

+ CO2

hoc 2HN-R-NH2(diamine)

C 3 loi decarboxylase quan trng:

lysine decarboxylase (LDC) ornithine decarboxylase (ODC) arginnine decarboxylase (ADC) / arginine dehydrolase (ADH)

lysine

lysinedecarboxylase

Cadaverine (diamine) + CO2

ornithine

ornithine decarboxylase

Putrescine (diamine) + CO2

arginine decarboxylase

arginine
arginine dehydrolase

Putrescine (diamine) + CO2

Mi trng:
Decarboxylase Basal medium

pH=6.0
Bromocresol purple

Hp kh trng mi trng => phn vo cc ng nghim

Cy vi sinh vt b sung du khong hoc parafin 370C trong 24 gi n 4 ngy

c kt qu:
Kim tra (+): vn c v c mu tm. Kim tra (): trong, mu vng.

bromocresol purple