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3.30. Phng php nh lng Bacillus cereus gi nh trn a thch (ISO 7932:2004) 3.30.1. Phm vi p dng Phng php nh lng Bacillus cereus gi nh c th p dng cho cc sn phm dng cho con ngi v thc n chn nui v cc mu mi trng trong khu vc sn xut v x l thc phm. 3.30.2. nh ngha Bacillus cereus gi nh l nhng vi sinh vt hnh thnh cc khun l in hnh trn b mt ca mi trng cy chn lc v cho phn ng khng nh dng tnh di cc iu kin c quy nh trong tiu chun ny. 3.30.3. Nguyn tc Cy mt lng mu th c nng pha long thch hp nu sn phm ban u dng lng hoc mt lng huyn ph ban u nu sn phm cc dng khc ln b mt cc a mi trng thch chn lc, cc a iu kin hiu kh 18 48 gi/ 30oC. Tnh s lng Bacillus cereus trong 1ml hay trong 1 gam mu t s khun lc khng nh thu c trn cc a cc nng pha long chn sao cho kt qu c ngha v c khng nh theo php th quy nh. 3.30.4. Mi trng ha cht: Dung dch pha long Mi trng thch MYP Thch mu
3.30.5. Thit b chnh: B iu nhit 41.5 1.0oC hoc t m 41.5 1.0oC. B iu nhit c th hot ng t 44 n 47oC T m 30.0 1.0oC. 3.30.6. Quy trnh 3.30.6.1. Chun b mu: (theo mc 3.27. s tay phng php vi sinh) 3.30.6.2. Pha long mu: (theo mc 3.27. s tay phng php vi sinh) - Chuyn 1 phn mu vo 9 phn dung dch pepton saline v trn u. - mu trng thi ng yn trong 20 30 pht 18 27oC (nhit phng) trc khi ng nht mu bng cch lc u.

3.30.6.3. Cy mu: - Ht 1ml dch cy ln b mt a thch MYP ln (140mm) hoc ln khp b mt ca 3 a nh (90mm). Trong c hai trng hp, chun b cho php xc nh kp s dng 2 a ln hoc 6 a nh.
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04.2-CL4/ST - S tay phng php Kim nghim vi sinh

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- Trang kh dch mu bng que cy hnh tam gic c thanh trng, c gng khng chm vo mp a, cc a c np y cho n kh trong khong 15 pht nhit phng th nghim.

- Lt p cc a v 30oC/ 18 24 gi. Nu khng th nhn r cc khun lc th thm 24h. 3.30.6.4. m khun lc. - Chn cc a 2 nng pha long lin tip c t hn 150 khun lc. m cc khun lc Bacillus cereus gi nh trn mi a (khun lc ln mu hng v thng c bao quanh bi mt vng kt ta), nu c t hn 15 khun lc c trng trn cc a th c th ly s m c tnh. Ghi ch: Nu cc a cha nhiu vi sinh vt ln men ng Mannitol dn n sinh acid, th mu hng c trng ca khun lc Bacillus cereus c th b nht i hoc b bin mt hon ton. Mt s khun Bacillus cereus ch sinh t hoc khng sinh Lecitinase. Cc khun lc thuc cc chun ny s khng c vng kt ta bao quanh do cn c th khng nh. 3.30.6.5. Khng nh:

Chn v lc khun lc khng nh: Nu trn a c t hn 5 khun lc th ly tt c cc khun lc gi nh c mt em khng nh. Nu trn cc a, cc khun lc mc qu dy v khng th chn cc khun lc phn lp tt th cy ria 5 khun lc gi nh ny ln cc a MYP 18 24 gi/ 30oC. Chn trn mi a t nht 1 khun lc c mu hng phn lp tt. Th hng cu trn thch mu. Cy ria, cy m su hoc chm cc khun lc chn ln mt thch mu cu sao cho th hin c tt phn ng hng cu, 24 2 gi/ 30oC. Gii thch phn ng sinh ha Bng 1: Cc kt qu th Php th Thch MYP Th hng cu Kt qu khng nh Bacillus cereus gi nh Hnh thnh khun lc mu hng c bao quanh bi mt vng kt ta Phn ng dng tnh ( rng ca vng hng cu c th thay i)

3.30.7. Biu th kt qu: Khng c khun lc < 1 vi sinh vt trong 1ml cc sn phm dng lng. < 1/d vi sinh vt trong 1g sn phm cc dng khc, trong d l h s pha long.
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