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  • Capillary Electrophoresis by Sunil Budke

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    This was a small presentation I made to the class on Capillary electrophoresis. The system shown here is the one I used at Therapeutic Drug Monitoring Center - Sion Mumbai. It is a auto timed presentation where slides and points keep changing in the background at preset timings in Sync with my talk. I know that without the talk this slide show may not be that useful but still I thought of sharing it with you guys. Hope you can make something out of it.

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    This was a small presentation I made to the class on Capillary electrophoresis. The system shown here is the one I used at Therapeutic Drug Monitoring Center - Sion Mumbai. It is a auto timed presentation where slides and points keep changing in the background at preset timings in Sync with my talk. I know that without the talk this slide show may not be that useful but still I thought of sharing it with you guys. Hope you can make something out of it.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    143 views34 pages

    Capillary Electrophoresis by Sunil Budke

    Uploaded by

    sunilbudke
    This was a small presentation I made to the class on Capillary electrophoresis. The system shown here is the one I used at Therapeutic Drug Monitoring Center - Sion Mumbai. It is a auto timed presentation where slides and points keep changing in the background at preset timings in Sync with my talk. I know that without the talk this slide show may not be that useful but still I thought of sharing it with you guys. Hope you can make something out of it.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 34

    CAPILLARY

    ELECTROPHORESIS
    BASIC CONCEPTS & HARDWARE

    by Sunil Budke
    CE by SUNIL BUDKE
    11/02/21 18:40

    Capillary tube

    Destination Vial
    Source vial

    +
    _
    CE by SUNIL BUDKE
    11/02/21 18:40

    +
    _
    CE by SUNIL BUDKE
    11/02/21 18:40

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    CE by SUNIL BUDKE
    11/02/21 18:40
    CE by SUNIL BUDKE
    11/02/21 18:40
    CE by SUNIL BUDKE
    11/02/21 18:40
    CE by SUNIL BUDKE
    11/02/21 18:40
    CE by SUNIL BUDKE
    11/02/21 18:40
    CE by SUNIL BUDKE
    11/02/21 18:40
    CE by SUNIL BUDKE
    11/02/21 18:40

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    CE by SUNIL BUDKE
    11/02/21 18:40

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    CE by SUNIL BUDKE
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    CE by SUNIL BUDKE
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    +
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    CE by SUNIL BUDKE
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    +
    _ N
    +
    + _N
    CE by SUNIL BUDKE
    11/02/21 18:40

    +
    _ N
    +
    + _N
    CE by SUNIL BUDKE
    11/02/21 18:40

    _ N ++
    _ N +
    CE by SUNIL BUDKE
    11/02/21 18:40

    +
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    CE by SUNIL BUDKE
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    _ N ++
    _ N
    CE by SUNIL BUDKE
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    _ N
    ++
    _ N
    CE by SUNIL BUDKE
    11/02/21 18:40

    _ N
    ++
    _ N
    CE by SUNIL BUDKE
    11/02/21 18:40

    +
    _
    Electropherogram from CE
    CAPILLARY
     Made of fused silica
     Polyamide coating for protection
     Pyrex or Teflon capillaries are available
     Effective length 10 - 100 cm
     Internal diameter (ID) 20 -100 micro meter
     C8 and C18 coated capillaries are
    available
    SAMPLE INTRODUCTION
    Electrokinetic Injection
    5kv for 5 – 10 sec

    Gravimetric (Syphonic)
    Sample vial held at higher level

    Differential pressure
    Pressure applied on sample vial or vacuum applied on
    Destination vial
    DETECTORS
     Conductivity Detectors
     UV Detectors
     Fluorescence Detector
     Electrochemical Detector
     Mass Spectrometer
    CE by SUNIL BUDKE
    11/02/21 18:40

    DISCUSSION
    References
     Capillary Electrophoresis
    -by Dale R. Baker

     Capillary Electrophoresis for Food Analysis – Method


    Development
    -by Richard A. Frazier; Jennifer M. Ames and Harry E. Nursten

     Principles and Practice of Bioanalysis


    -by Richard F. Venn

     McGraw Hill - Year book of Science and Technology – 2002


    -Capillary electrophoresis
    Thank You

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