32-Tìm Hiểu Quy Trình Sản Xuất Một Số Chế Phẩm Probiotic Và Prebiotic

TRNG I HC CNG NGHIP THNH PH H CH MINH
VIN CNG NGH SINH HC V THC PHM

TIU LUN MN DINH DNG HC

TI S 32: TM HIU QUY TRNH SN XUT MT S CH PHM
PROBIOTIC V PREBIOTIC

TP HCM, thng 03 nm 2014.
ti s 32: Tm hiu quy trnh sn xut mt s ch phm probiotic v prebiotic

MC LC
M U
NI DUNG
1. CH PHM PROBIOTIC ................................................................................................... 1
1.1. Tng quan ...................................................................................................................... 1
1.1.1. Khi nim ............................................................................................................... 1
1.1.2. Cc th h probiotic ............................................................................................... 1
1.1.3. Li ch ca probiotic .............................................................................................. 1
1.1.4. Hot ng ca vi sinh vt probiotic ....................................................................... 1
1.1.5. ng dng ca ch phm probiotic ......................................................................... 2
1.1.6. Cc dng ch phm probiotic ................................................................................. 2
1.2. Sn xut ch phm probiotic ......................................................................................... 2
1.2.1. Chn la vi sinh vt probiotic ................................................................................ 2
1.2.2. Quy trnh sn xut tng qut .................................................................................. 3
1.2.3. Thuyt minh quy trnh sn xut ............................................................................. 3
1.2.4. Mt s ch phm probiotic .................................................................................... 4
2. CH PHM PREBIOTIC .................................................................................................... 6
2.1. Tng quan ...................................................................................................................... 6
2.1.1. Khi nim ............................................................................................................... 6
2.1.2. Li ch v tc hi ca prebiotic i vi sc khe .................................................. 6
2.2. Sn xut ch phm Prebiotic ......................................................................................... 6
2.2.1. Sn xut FOS ......................................................................................................... 7
2.2.2. Sn xut GOS ....................................................................................................... 10
3. S KHC NHAU GIA PROBIOTIC V PREBIOTIC ................................................ 12

KT LUN
PH LC
TI LIU THAM KHO

LI M U

Cng vi s pht trin ca x hi, khoa hc cng ngh th cm t ch phm sinh hc
khng cn qu xa l vi mi ngi. Nhc ti ch phm sinh hc probiotic, prebiotic ta thy ngay
c nhng li ch m chng mang li cho sc khe con ngi cng nh li ch trong chn nui,
trng trt. Ch phm sinh hc ngy nay c ng dng rng ri trong nhiu lnh vc ca i
sng nh y t, lm p, sn xut thc phm, s dng trong nng nghip. Tuy nhin, chng ta vn
cha bit v hiu r c v ngun gc, quy trnh sn xut ca nhng ch phm . Vn ny
s c trnh by trong ti Tm hiu quy trnh sn xut mt s ch phm probiotic v
prebiotic. Trong phm vi mn Dinh dng hc, em ch tm hiu ti ny trn i tng ngi
v c trnh by trong phn Ni dung bn di.


1

NI DUNG
1. CH PHM PROBIOTIC
1.1. Tng quan
1.1.1. Khi nim
Probiotic l vi sinh vt sng trong khi c qun l ph hp v mt em li li ch
cho sc khe trn vt ch (FAO 2001). C th hn, probiotic l cc vi khun sng c li ng
rut, khi b sung vo c th em li cc li ch sc kho cho con ngi nh ci thin h vi sinh
ng rut v chc nng ca rut.
1.1.2. Cc th h probiotic
Sn phm ln men t nhin: sa chua, da chua, ph mai, kim chi,c dng c li v
mt dinh dng, nhng cha c tnh h tr tr liu mt cch r rng.
Th h th 1, probiotic khng bao (non-coated): c cc sn phm nh: Antibio, Abio Gran,
Biolac, Bio - Acimin, Bio - Baby,; vi khun c phn lp v tng s lng s dng,
nhng vn l vi khun khng bao. Nhc im: d b hao ht khi bo qun v s dng.
Th h th 2, dng bao tan trong rut (enteric- coated): vi khun cha trong nang c lp
bo v ch tan trong rut.
Th h th 3, dng bao vi nang (micro-encapsulated): nh Lactomin, gip vi khun qua d
dy tt hn. Nhc im: tan chm trong rut.
Th h th 4, bao kp (DuolacTM): nh Kidlac, Lacclean Gold, Chewable 7.1, Yamyam,
probiotic c bao 2 lp, hiu qu cao nh kh nng sng cao n rut v ng thi khc
phc nhc im phng thch chm rut nh cch bao cc th h c, nh lp bao th 2 (lp
bao peptide) c tnh thc y phng thch ti ch.
1.1.3. Li ch ca probiotic
Probiotic c nhng li ch nh sau: tng cng kh nng tiu ha lactose v hot ng ca
cc enzyme khc; tr tiu chy do khng sinh; tr vim ng tiu ha; c ch v tiu dit vi
khun c hi; gim nguy c ung th rut kt; iu ha min dch, gim phn ng min dch gy
vim; tng cng sc khe sinh sn ca ph n.
1.1.4. Hot ng ca vi sinh vt probiotic
Cnh tranh v tr dnh bm v ngun dinh dng vi vi sinh vt gy bnh.
Sn sinh cc cht khng khun.
Sn sinh cc enzyme tiu ha.
Kch thch h min dch vt ch.
Cn bng h vi sinh vt ng rut.
c ch c ch kim sot mt ti hn ca h vi sinh vt.

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Chuyn ha cc cht nhim mi trng RNA, protein.
Tng khng th.
Sn xut cc sn phm th cp.
Sn xut cht khng khun, enzyme tiu ha
1.1.5. ng dng ca ch phm probiotic
1.1.5.1. Trong y hc v thc phm
S dng vi khun mui chua rau qu, chua thc n gia sc (lm chn sinh hc cc loi
qu): To c sinh khi vi khun c ch, t c sinh vt gy thi. Gy chua, to hng v thm
ngon cho sn phm. Chuyn rau qu v dng chn sinh hc do m hiu sut tiu ha tng.
Mt s chng dng sn xut sa chua c: Lactobacillus bulgaricus, Strepticoccus faecalist
Mt s chng c thm vo sa bt: Lactobacillus, Bifidobacterium Ti nhng nc pht
trin, cc nh sn xut, ch bin ng dng b sung Probiotics vo nhiu loi thc phm nh
sa chua n, phomt, kem.
Cha bnh ng rut: Php sn xut v a ra th trng t hng chc nm nay mt
sn phm mang tn Biolactyl chuyn tr tiu chy bng nhiu khun Lactic. Vt liu sinh hc.
1.1.5.2. Trong nng nghip
Probiotic c ng dng rng ri trong nng nghip: sc sn, thy sn, trng trt Probiotic
gip ci thin sc khe ca ng vt, gip tng trng, gim t l cht non v ngn chn tc nhn
gy bnh. S lm dng khng sinh trong ngnh chn nui v kh nng khng khng sinh
lm tng mi quan tm n probiotics.
1.1.6. Cc dng ch phm probiotic
Probiotic c lnh (yophilis): khi h nhit xung tht thp vi khun s tr nn kh nh
bt nhng khng b tiu dit. Chng s sng tr li khi c a vo c th.
Vin nang (capsule): thng c hai hoc bn loi phi hp vi nhau nh L.casei,
L.acidophilus, L.bulgaricus, L.rhamnosus, Streptococcus thermophilus, B.lactis, B. longum.
Bt (poudre): dng bt pha trong nc. Nh lu li bao t trong thi gian ngn nn khi n
rut, mt s lng ln vi khun vn cn sng.
Sa chua tr liu: Nn ung trc ba n, c th cha mt t l probiotic rt cao (50 t
CFU/ liu).
1.2. Sn xut ch phm probiotic
1.2.1. Chn la vi sinh vt probiotic
Hin nay, cc ch phm probiotic ang c sn xut nhiu u ch yu cung cp cho
ng vt nui, gia sc, gia cm ring i vi ngi th ang rt hn ch. V nhng ch phm
s dng cho ngi nh hng trc tip n sc khe con ngi, vic sn xut cc ch phm ny

3

cn phi lun cn thn v ch tng giai on. V vy khi sn xut trc ht cn chn ng loi
vi sinh vt, sau cn kim sot cht ch cc yu t c th nh hng n cht lng sn phm.
Khi chn la vi sinh vt probiotic cn quan tm n cc yu t sau: kh nng bm dnh ln
thnh rut; khng sinh c t, khng gy hi vt ch; to sn phm vt ch s dng c; c kh
nng sinh trng sinh sn tt; d nui cy, s lng t bo qun th ln; chu c pH thp;
sng st c khi bao gi (bo t); nhit .
1.2.2. Quy trnh sn xut tng qut
Di y l quy trnh sn xut tng qut ch phm probiotic dng kh v dng lng.

S 1: Quy trnh sn xut ch phm probiotic.
i vi cc ch phm probiotic, khng c mt quy trnh c th sn xut mt loi ch phm
c th c cng b. V vy em xin trnh by quy trnh tng qut sn xut ch phm probiotic v
tm hiu v mt s ch phm c th l Viabiovit v Kidlac.
1.2.3. Thuyt minh quy trnh sn xut
Nhn ging
Ln men
Dch men
Chuyn ha
n nh
C nh vi sinh vt
Sn phm
dng lng
Ging
Sn phm
dng kh
ng gi ng chai

4

Chun b ging
Chng vi sinh vt sau khi c tuyn chn, thun ha gip vi sinh vt tng kh nng thch
nghi vi iu kin thc t v lnh vc phc v. Tip theo, chng vi sinh vt c em i nhn
ging to thnh ging s dng cho sn xut. H thng lu gi ging vi sinh thun chng phi
c bo qun theo tiu chun k thut c bit. Cn kim tra mt v trng thi hot ng ca
vi sinh vt. Cc thng s phi c ghi nhn v bo co hng gi.
Nhn ging
Mi trng dch th chun b cho qu trnh nhn ging m bo y cc cht dinh
dng, cc nguyn t a lng v vi lng. Trong mi trng ny, vi sinh vt sinh trng, pht
trin nhanh chng v dn n nh thch nghi trc khi c a vo quy trnh ln men. Nhn
ging cn m bo to ra v duy tr ngun ging thun chng mang y cc u th ca ging
gc.
Ln men
Ty vo mc ch sn xut v chng vi sinh vt s dng chun b cc thng s nh
nhit , pH, mi trng ln men ph hp.
X l n nh hot tnh men v c nh vi sinh vt
Vi sinh vt s c a vo mi trng c bit. Mi trng ny c b sung v thay i
cc t cht v iu kin cn thit cho qu trnh hnh thnh bo t. Giai on ny s hnh thnh
nn bo t, t bao tim sinh.
Sn xut men kh
Dch men chun b c em i ng kh to khi vi sinh vt. Khi vi sinh vt ny
c nghin thnh dng bt, c nng vi khun cao. Sau bt c trn, nn. Cui cng men
vi sinh c ng gi trong nito, nhm mc ch bo qun, ta c thnh phm.
1.2.4. Mt s ch phm probiotic
1.2.4.1. Ch phm Viabiovit
Viabiovit c thnh phn l cc vi khun lnh tnh h Lactobacillus (Lactobacillus
sprorogenes, Lactobacillus kefir, Lactobacillus acidophilus) l thnh phn chnh v cng quan
trng v khng th thiu trong cc ch phm men vi sinh kt hp vi cc vitamin nhm B v
vitamin PP c tc dng tng cng kh nng tiu ho v hp thu tt cc cht b dng cho tr.
Sn phm c phi hp 3 chng vi khun trong h Lactobacillus rt c li cho ng rut. Vi
hm lng vi khun ln v dng ng kh nn kh nng sng bo tn lu khi c bo qun
nhit thng. Cc chng vi sinh vt ny s gip cn bng h thng vi khun c ch trong
ng rut, gip thc y qu trnh tiu ho v hp thu dinh dng.

C ch tc dng ca Viabiovit: lm tng cng hp thu thc n nh cc hot tnh ca 3
chng vi khun c ch.

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- To mi trng axit nh tit ra axit lactic, y l mi trng trng thun li cho h vi
sinh ln men ng rut pht trin v ngc li, c ch s pht trin ca vi khun gy
bnh dng Gram(-).
- Tit ra cc cht dit khun nh: acid acetic, acid benzoic
- Thc y qu trnh tng hp vitamin v mt s acid amin cn thit cho c th, do rt
c li cho qu trnh tiu ho v hp thu.
- Tc dng ca mt s vitamin nhm B v PP: nng cao sc khng ca c th, c bit
gip tng cng kh nng tiu ho v hp thu tt cc cht b dng cho tr s sinh.
- Cn bng ri lon h vi khun ng rut do dng khng sinh. Gip to cm gic ngon
ming v tng cng tiu ho, dng tt cho nhng ngi ri lon tiu ho (tiu chy, to
bn, i trng mn tnh, y hi) khng ch vi tr em m cn c tc dng tt vi nhng
ngi c nguy c bnh tim mch v chng lo ho tui gi.
1.2.4.2. Ch phm Kidlac
Ch phm Kidlac l ch phm vi sinh dnh cho tr em. L s kt hp synbiotic gia 4
chng probiotic vi prebiotic s dng cng ngh probiotic bao kp. Sn phm thuc th h
probiotic th 4. Probiotic c bao 2 lp, hiu qu cao nh kh nng sng cao n rut, c tnh
thc y phng thch ti ch nh lp bao th 2. Probiotic nh lp bao polysaccharid gip bo
m sng tt trong qu trnh sn xut v bo qun, lp bao protein c c tnh ng vn trong
mi trng acid gip chng c tc ng khi qua dch d dy v mui mt n rut. Ti
rut, nh lp protein c phn tn tt trong pH trung tnh hoc hi kim rut, probiotic s phng
thch tt nh sn sinh nhanh chng pht huy ti a tc dng.
Kidlac c phi hp nhiu chng, c tc dng tt hn dng ring l. Kidlac l s kt hp
ca bn chng vi khun: Lactobacillus acidophilus (100 billion cfu/g), Bifidobacterium longum
(50 billion cfu/g), Bifidobacterium breve (50 billion cfu/g), Enterococcus faecium (100 billion
cfu/g).
Hiu qu khi s dng Kidlac:
- Gim nhanh chng cc ri lon ng rut do bnh l v/hoc dng khng sinh ko di
hoc nhim nhng loi khun gy hi, thuc ha tr liu, gy nn cc triu chng nh
tiu chy, vim rut, chng bng, y bng, i phn sng, ri lon tiu ha, tng s thi
ra trong rut.
- Ci thin cc tnh trng mn tnh do ri lon ng rut thng xuyn v lu di nh: to
bn, vim i trng, thng xuyn b trc trc tiu ha v tiu chy, b tiu chy khi ung
sa do khng dung np lactose
- Ti lp cn bng t nhin trong h tiu ha, kch thch s pht trin ca cc vi khun c
li cho sc khe, gip ng rut khe mnh.
- Gip gim nhim khun v d ng i vi cc khng nguyn c trong thc phm.
- Tng kh nng hp th dinh dng, cung cp cc vitamin cho c th nhm nng cao sc
khe, tng sc khng ca c th.

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- Gip tng cng kh nng min dch, phng nga v khng nhiu bnh khc nhau.
- Cung cp cc vitamin cho c th nhm nng cao sc khe, colostrum gip tng kh nng
min dch v DHA gip pht trin tr no.
2. CH PHM PREBIOTIC
2.1. Tng quan
2.1.1. Khi nim
Prebiotic c nh ngha nh l mt thnh phn thc phm khng tiu ha c tc ng
c ch n vt ch bng cch kch thch c chn lc s pht trin hoc hot ng ca mt hay
mt s lng gii hn cc loi vi khun trong rut kt v do gip ci thin sc khe ca vt
ch (Gibson v Roberfroid, 1995). Prebiotic ch cc loi cht x ha tan c mch ngn, ch yu
l cc hp cht oligosaccharide.
2.1.2. Li ch v tc hi ca prebiotic i vi sc khe
Prebiotic l ngun thc n cho probiotic, to iu kin cho li khun pht trin mnh m
v ci thin h tiu ha ca c th. Prebiotic khng b tiu ha d dy, rut non nn n cn
nguyn khi xung rut gi, prebiotics nui dng vi khun rut gi v lm tng hot ng ca
chng y.
Prebiotic tng cng hp thu canxi, magie v cc cht khong khc; nng cao v tng
cng h min dch ca c th; gim nguy c ung th trc trng v ngn nga ung th rut kt
giai on u; duy tr mc ng huyt, h tr ngi bnh tiu ng, gim cholesterol xu, h
tr n nh huyt p, duy tr thoi mi cho ng rut, ngn chn s xm nhim ca mt s vi
khun gy bnh, chng su rng, gim nguy c d ng tr em, h tr tiu ha d dng hn v
hn ch cc trng hp kh tiu do y hi, to bn.
Tuy nhin, prebiotic ch c tc ng tch cc ti c th c sn cc vi sinh vt hu ch,
bn thn prebiotics khng sinh ra cc vi sinh vt ny. S dng nhiu prebiotics gy tnh trng
y hi, i tiu nhiu hn 2 ln/ngy. Cc loi ng c ngun gc t FOS c th kch thch s
tng trng ca vi khun Klebsiella l mt vi khun gy bnh ng rut. Tiu th prebiotic
lm tng vi khun to kh gas trong h tiu ha, do cc sn phm mi pht trin gn y
thng hng ti vic y mnh s pht trin ca vi khun khng to kh. Prebiotic c tc dng
khc nhau ln cc i tng khc nhau. V d FOS khng nh hng n s hp thu canxi
thanh nin nhng li kch thch s hp thu ny thiu nin. V vy cn ch khi s dng
prebiotics.
2.2. Sn xut ch phm Prebiotic
Prebiotic c sn xut ch yu bng bn cch:
- Chit tch trc tip t nguyn liu th thc vt nh: inulin t rau dip xon,
oligosaccharide soybean t u tng
- Thy phn c kim sot: thy phn c kim sot polysaccharide bng enzyme, c th s
dng phng php sc k tinh sch prebiotic nh fructooligosaccharide t inulin.

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- Glycosyl ha: qu trnh tng hp bng cch s dng enzyme thu phn to
oligosaccharide t cc disaccharide v c tinh sch bng qu trnh sc k nh GOS t
lactose, FOS t sucrose
- Cc qu trnh ha hc: s chuyn i xc tc t cc cacbohydrate nh Lactulose t s
ng phn ha lactose trong mi trng kim.

Hnh 1: Quy trnh sn xut ca cc oligosaccharide khng tiu ha.
(phng theo Sako v cng s, 1999)
2.2.1. Sn xut FOS
2.2.1.1. Gii thiu v FOS
FOS (fructooligosaccharide) l cht x ha tan, l cht to ngt nng lng thp. FOS kch
thch tiu ha, gim cholesterol, phospholipid, triglyceride, chng bo ph, an ton cho ngi
bnh tiu ng, nga su rng, tng kh nng hp th cc ion Ca
2+
, Mg
2+
.
FOS l nhng oligosaccharide c 1 phn t sucrose lin kt vi 1, 2 hay 3 gc fructosyl
bng lin kt 2,1 glucoside.
Cng thc cu to ca FOS:

Hnh 2: Cng thc cu to ca FOS
Trong t nhin, FOS c nhiu trong cc loi c qu nh hnh ty, ti, to, mng ty, hoa
atiso, chui. Mt ong cng l ngun cha FOS. Tuy nhin, FOS trong t nhin c nng thp,

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enzyme chuyn ha gc fructose b gii hn bi iu kin thi tit. V vy FOS c sn xut
trong cng nghip bng qu trnh thy phn polysaccharide hoc tng hp t ngun nguyn liu
sucrose v enzyme chuyn ha to FOS t vi sinh vt, ch yu l nm mc.
Syrup FOS thng mi gm 25 30 % 1 kestore (GF
2
), 10 15 % nystose (GF
3
) v 5 10 %
1
F
- fructofuranosylnystose (GF
4
) v 25 30 % glucose nh mt sn phm ph (Kim v cng s,
2000).
2.2.1.2. Quy trnh sn xut FOS trong cng nghip

S 2: Quy trnh sn xut FOS gin on trong cng nghip. (phng theo Yun, 1996)
Nui cy t bo
Chit tch enzyme
Phn ng enzyme
Tinh sch
C c
Tit trng
Dung dch
saccharose
Sn phm

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S 3: Quy trnh sn xut FOS lin tc trong cng nghip. (phng theo Yun, 1996)
Trong hai phng php trn, phng php lin tc c nhiu u im ni tri v kh nng
cng nghip ha cao hn.
2.2.1.3. Cc yu t nh hng n sn xut FOS
Loi vi sinh vt thng s dng lm ngun enzyme sn xut FOS l A. niger,
Auerobasidium pullulans v Auerobasidium japonicus (tt nht sn xut FOS loi 1
F
)
Enzyme t nhng ngun khc nhau thng c khong dao ng t 232 kDa (Imamura v
cng s, 1994) n 246 kDa (Hayashi v cng s, 1991).
Qu trnh sn xut FOS cn nh hng bi cc yu t nh: pH, nhit . pH ti u sn
xut FOS l t 5 n 6, nhit ti u t 50
o
C n 60
o
C. Nhng gi tr ny c th thay i ty
theo ngun enzyme. Nng sut ti a ca FOS th khc nhau theo ngun v phn ng c thit
lp, t 42% (Kim v cng s, 1998) t Bacillus macerans n 80% (Madlova v cng s, 1999)
t Auerobasidium pullulans v A. niger.
Mc d phng php sn xut FOS tn ti c thit lp rt tt nhng nhiu nh nghin
cu vn tip tc tm ra cch lm tng nng sut v hiu qu ca phn ng ti quy m cng
nghip, cng nh cc phng php n gin ha v thc y phn ng ca chnh n.
Ngun enzyme khc nhau v iu kin phn ng c s dng trong cc n lc ti
u ha k thut c nh enzyme.
Nui cy t bo
C nh t bo
Phn ng theo ct
Tinh sch
C c
Tit trng
Dung dch
saccharose
Sn phm

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Chien v cng s (2001) hon thnh sn xut FOS qua vic c nh t bo ca
A.japonicus ti gluten trong phn ng gin on. Mt gam si c c nh c vi 100ml
ca 400g l
-1
dch ng saccharose. Lm tng 61% nng sut sn xut FOS sau 5 gi, sn phm
gm ch yu l 1 kestose v nystose. S tp trung ca saccharose v t bo trong gluten c
nh hng ng k n tc phn ng ca enzyme.
2.2.2. Sn xut GOS
2.2.2.1. Gii thiu v GOS
GOS (galactooligosaccharide) l mt prebiotic c ngun gc t ng vt, c chit sut
t lactose c trong sa b, d. Mt s GOS c s dng nh mt loi cht to ngt nng lng
thp, thnh phn ca thc phm v cht thm vo m phm.
Cu trc ha hc ca GOS gm galactose v lactose lin kt vi nhau.
GOS bn hn nhiu so vi FOS. Chng khng thay i sau khi x l 120
o
C trong 10
pht ti pH bng 3 v 100
o
C trong 10 pht ti pH bng 2.
2.2.2.2. Quy trnh sn xut GOS
GOS c sn xut t syrup ng lactose nh enzyme thy phn - galactosidase
(Gibson v cng s, 2000). - galactosidase c th c tch ra t thc vt nh o, m, hnh;
ng vt t rut, no, m da; vi sinh vt nh nm men, vi khun, nm mc. Vi khun thng cho
nng sut cao hn khi sn xut cc sn phm vi mc ch thng mi (Shukla, 1975).

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Quy trnh sn xut GOS c trnh by bn di.

S 4: Quy trnh sn xut GOS trong cng nghip.
(phng theo Matsumoto, 1990 v Sako cng cng s, 1999)
2.2.2.3. Cc yu t nh hng n sn xut GOS
Ta c phn ng to thnh GOS nh sau:

Ph thuc vo ngun thu enzyme - galactosidase m kt qu sn xut GOS t lactose c
th khc nhau v sn phm cui cng v nng sut sn phm. Tng lng sn phm
oligosaccharide ph thuc vo ngun enzyme v c th thay i t 1 n 45 % tng lng ng
hin c (Zarate v Lopez Leiva, 1990). V oligosaccharide lin tc c tng hp v gim st,
Enzyme c th thay i trong khong t 36kDa (Tsumura v cng s, 1991) n 362 kDa
(Dumortier v cng s, 1994). pH ti u dao ng t 4 n 9 (Tsumura v cng s, 1991). Nhit
ti u t 40
o
C (Shin v Yang) n 95
o
C (Hansson v Aldercreutz, 2001) cho
Galactosidase t Pyrococcus furiosus. Nhit qu cao hay qu thp u nh hng n
hot tnh ca enzyme. Nh vy khi la chn pH cho enzyme cn quan tm n c nhit la
chn. Khi tng nng enzyme th GOS tng hp tng.
Phn ng enzyme

Lm mt mu
Kh khong
Lc
C c Lm kh
6GOS
- galactosidase t
A.oryzae v/hoc S.
thermophilus
GOS dng
lng
Dch Lactose
GOS dng
bt
4GOS
- galactosidase t
B.clrculans hoc
Cryptococcus laurenill

12

Khi tng nng lactose, lng GOS tng hp tng. Khi lactose t n gii hn ti a,
lng GOS to thnh khng tng.
nh hng ca nng ng glucose v galactose: s c mt ca cc ng n nh
hng khng tt n qu trnh hnh thnh GOS, lm gim lng GOS.
Thi gian cng nh hng n s tng hp GOS. thi im ban u, lng GOS to
thnh chm v tc tng nhanh. Sau lng GOS to thnh cc i v cui cng lng GOS
gim dn theo thi gian giai on sau.
3. S KHC NHAU GIA PROBIOTIC V PREBIOTIC
Probiotic Prebiotic
L nhng vi khun sng c li ng rut. L dng c bit ca cht x thc phm.
C th b tiu dit bi nng, lnh hoc thi
gian tri qua.
Khng b tc ng bi nng, lnh, axit hay thi
gian.
T mt n mt s loi vi khun c thm
vo ng rut.
Nui hng nghn loi vi khun c li c sn
trong ng rut.
Tm thy trong thc phm ln men nh sa
chua, da chua.
L cht t nhin, c tm thy hng nghn
loi thc vt.
C th tc ng n vi khun c hi bng cch
cn tr chng.
Nui dng mi trng trong ng rut, ni
l k th ca vi khun c hi.

Bng 1: s khc nhau gia Probiotic v Prebiotic

KT LUN
Ch phm sinh hc lun l vn ng quan tm trong x hi hin nay ni chung v trong
gii sinh hc thc phm ni ring. Li ch t cc ch phm probiotic v prebiotic l rt ln.
hiu r v su hn cc vn xung quanh n, ta cn u t mt cch nghim tc thi gian v
cng sc.
Trn y l nhng tm hiu v trnh by ca em v ti Tm hiu quy trnh sn xut mt
s ch phm probiotic v prebiotic. V kin thc v kh nng tm hiu c hn nn em khng th
trnh by y v chi tit ni dung ca ti c. Bi trnh by cn kh s si, em s c gng
tm hiu k hn v nhng vn ny.

PH LC

Hnh a : Cu trc ca FOS (phng theo Spiegel v cng s, 1994)

Hnh b: Probiotic sau khi a vo rut ngi. Hnh c: h vi sinh vt trong rut ngi.

Hnh d: 4 th h probiotic c tc dng tr liu. Hnh e: ng i ca prebiotic trong c th ngi

Bng g. Cc thng s ti u cho hot ng ca cc loi vi sinh vt trong sn xut FOS.

Hnh h: Cc thng s ti u cho hot ng ca cc loi vi sinh vt trong sn xut FOS.

TI LIU THAM KHO

[1] Development and Application, G.R. Gibson and R.A. Rastall, Copyright 2006 John Wiley &
Sons, Ltd. Trang 13, 30 42.
[2] Hanbook of prebiotics and probiotics ingredients, edited by Susan sungsoo cho E. Terry
finocchiaro, copyright 2010 by Taylor and Francis Group, LLC. Trang 14, 15, 194.
[3] Bi ging Cng ngh probiotic, ging vin L Phng Chung, Vin Cng ngh sinh hc v
mi trng, i hc Bch khoa H Ni nm 2013.
[4] Bi bo Probiotic th h th 4: Sn phm ca cng ngh bao kp, Gio s Nguyn Vn
Thanh, nm 2008. http://www.khoahocphothong.com.vn/newspaper/detail/3324/-probiotic-the-
he-thu-4:-san-pham-cua-cong-nghe-bao-kep.html
[5] http://www.cesti.gov.vn/su-i-ngu-n-tri-th-c/n-n-hi-u-ung-v-prebiotic.html
[6] http://chattrosinh.vn/tin-tuc/59/loi-ich-cua-probiotic.html
[7]http://www.thuocbietduoc.com.vn/hoi-dap-1-1-313/kha-nang-tang-hap-thu-cua-viabiovit.aspx
[8] http://www.prebiotin.com/prebiotics/health-benefits/
[9] http://www.prlog.org/10374468-prebiotics-vs-probiotics-whats-the-difference.html

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