Tng quan v h enzyme cellulase

Cng ngh sn xut

ng dng trong sn xut bioethanol
Ti liu tham kho
Tng quan v h enzyme cellulase
Enzyme Cellulase l mt phc h ezyme c tc dng thy
phn cellulose thng qua phn ct lin kt 1,4-- glucoside
trong cellulose to sn phm glucose cung cp cho cng
nghip ln men. Ngun thu cellulase ln nht hin nay l vi
sinh vt.
H Cellulase gm
- endo-(1,4)--D-glucanase (EC.3.2.1.4)
- exo-(1,4)--D-glucanase (EC.3.2.1.91)
- -glucosidases(EC.3.2.1.21)
hot ng phi hp thy phn cellulose thnh glucose.
Tnh cht enzyme



- Nhit thch hp hot ng 55
o
C,
bn 30-45
o
C
- Bn pH=5,5 v hot tnh cao pH= 6
- t nh hng bi cc dung mi hu
c (tr n- butanol)
- Cc ion kim loi v EDTA nng 4-
12mM nh hng lm gim hot tnh
enzyme.

C ch phn ng


Cc ng dng ca Cellulase

Ngnh cng
nghip
ng dng
Nng nghip Ch phm bo v thc vt, nng cao kh nng tng trng cy trng, bo v
t
Ha sinh Bioethanol, dung mi hu c, protein n bo, axit hu c
Cht ty ra Loi b cc cht bn trn cc loi vi bng th, khng gy nh hng h si
Ln men Ci thin qu trnh sn xut malt, gim nht ca dch nha, ci thin mu
sc v mi v ru vang
Thc phm Cht chng oxy ha, nng cao cht lng tinh bt v protein; ci thin
nht, kt cu, hng v ca nc hoa qu
Giy v bt giy H tr qu trnh nghin bt giy, gim lng cht ty ha hc, lng nc
tiu th, sn xut giy d phn hy sinh hc
Dt may Lm mm hng may mc, tng tnh n nh ca cc loi vi cellulose, ty
thuc nhum
Ngun enzyme:
ng vt: dch tit d dy b, cc nhm thn mm
Vi sinh vt: cc loi x khun, vi khun, nm si, nm
men
Trong thc t ngi ta thng thu nhn enzyme cellulase
t vi sinh vt. Cc chng vi sinh vt thng s dng:
Nm mc: Aspergillus niger, Aspergillus oryzae,
Aspergillus candidus
X khun: Actinomyces griseus, Streptomyces reticuli
Vi khun: Acetobacter xylinum, Bacillus Subtilis, Bacillus
pumilis
Cng ngh sn xut cellulase
Phi trn
Cng ngh sn xut cellulase
Cng ngh sn xut enzyme

Ln men lng

J. Zhuang, M. A. Marchant, S. E. Nokes, H. J. Strobel(2007). ECONOMIC ANALYSIS OF CELLULASE
PRODUCTION METHODS FOR BIOETHANOL Applied Engineering in Agriculture Vol.23(5): page 681,2007
Tinh sch
Ngun thu
nhn enzyme
(Trichoderma
harzianum)
Ly tm
Dch
chit
Kt ta bng
(NH
4
)
2
SO
4

Ly tm
Dch enzyme
th
Ly tm
Dch trong
Ta mui
(NH
4
)
2
SO
4
25%
Thm tch
loi mui
Qua ct Sephadex G200,
G50(30cm/h)
Enzyme tinh
sch
Thit b
Cc dng ch phm enzyme
Dng lng Dng rn
http://www.alibaba.com/promotion/promotion_cellulase-enzyme-promotion-list.html
http://www.21food.com/products/cellulase-663645.html


cellulase
Unikzyme CEL
Thng s k thut ch phm
Hot
solid product: 10000u/g, 20000u/g
liquid product: 10000u/ml, 20000u/ml
hot tnh theo phng php dns, n v l mgg / g.h.
pH 4.5 -6.0 (ti u pH5.0)
Nhit : 40-60 (ti u 55)
Bo qun 0-4
o
C
Thi gian 12 thng
iu kin s dng
Cng nghip thc n:0.1 % sn phm rn
Cng nghip giy: ty theo cng ngh
Novozyme
Cellulclast 1.5 LFG
Novozyme188(-glucosidase )
NovozymeNS50013(Cellulsase)
NovozymeNS5001(-glucosidase )
NovozymeNS50012(hn hp -glucosidase , xylanase)

ng dng trong sn xut bioethanol
Nguyn liu l vn ang c th gii quan tm
Bioethanol t ph phm nng,lm nghip l ngun nguyn liu
pht trin bn vng bn cnh cc phng php khc nh
biodiesel, biogas,
S dng cellulase trong qu trnh tin x l v thy phn
cellulose thnh glucose cho qu trn ln men ethanol.
Cc hng nghin cu hin nay tp trung vo ci thin qu trnh
tin x l v to chng sinh enzyme thy phn cellulase c hot
lc mnh
Sn xut bioethanol t b ma v
rm r
Tim nng ng dng
Xu hng pht trin ng dng:
Cng nghip nng lng
Cng nghip hng tiu dng

Cng ngh sinh hc l xu hng cng
ngh hin i cng vi pht trin bn
vng
Ti liu tham kho
1. J. Zhuang, M. A. Marchant, S. E. Nokes, H. J. Strobel(2007). ECONOMIC
ANALYSIS OF CELLULASE PRODUCTION METHODS FOR BIOETHANOL
Applied Engineering in Agriculture Vol.23(5): 679687,2007.
2. K.Karthikeyan, Research Scholar Junior Research Fellow, UGC NET Department of
Environmental Sciences Bharathiar university Coimbatore- 641 046- 2010.
3. SIBTAIN AHMED, AMMARA BASHIR, HUMA SALEEM, MUBSHARA SAADIA AND AMER
JAMIL-PRODUCTION AND PURIFICATION OF CELLULOSEDEGRADING ENZYMES
FROM A FILAMENTOUS FUNGUS TRI CHODERMA HARZI ANUM Pak. J. Bot., 41(3): 1411-
1419, 2009.
4. Ramesh Chander Kuhad,Rishi Gupta,and Ajay Singh; Review Article Microbial Cellulases and Their
Industrial Applications , Received 14 May 2011; Accepted 9 July 2011, SAGE-Hindawi Access to
Research, Enzyme Research, Volume 2011, Article ID 280696, 10 pages
5. QI XU, WILLIAM S. ADNEY, SHI-YOU DING AND MICHAEL E. HIMMEL, chap 3
CELLULASES FOR BIOMASS CONVERSION, Industrial Enzymes, 2007 Published by
Springer,P.O. Box 17, 3300 AA Dordrecht, The Netherlands.





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