Professional Documents
Culture Documents
CHP12
1)Which of the following regulatory agencies is charged with the regulation of plant pests and plants?
A)IND
B)USDA
C)EPA
D)USPTO
E)FDA
2)Phase ________ trials occur when a drug is tested on small groups of healthy individuals to determine the
proper dose and route of administration.
A)I
B)II
C)III
D)IV
E)V
3)Phase ________ trials occur when a drug is tested on several thousand people to determine its
effectiveness compared to current drugs on the market.
A)I
B)II
C)III
D)IV
E)V
4)Which U.S. regulatory agency oversees guidelines and laws that govern environmental applications of
biotechnology and other sciences?
A)EPA
B)USDA
C)FDA
D)NSF
E)NIH
5)Which of the following U.S. agencies oversees phase testing of drugs prior to their approval for clinical
uses?
A)FDA
B)USDA
C)NSF
D)EPA
E)NIH
6)________ are quality control regulations that govern procedures for human subject experimentation.
A)Quality assurance (QA)
B)Preclinical testing
C)Good manufacturing practices (GMPs)
D)Good clinical practices (GCPs)
E)Good laboratory practices (GLPs)
7)If you were developing a transgenic strain of pest-resistant watermelons, which federal agency would
primarily be responsible for regulating this biotech product?
A)DEP
B)USDA
C)EPA
D)FDA
E)DOT
8)Which federal agency oversees, regulates, and monitors the safety of biotech and pharmaceutical
products and medical devices during consumer use?
A)Environmental Protection Agency
B)National Science Foundation
C)Food and Drug Administration
D)U.S. Department of Agriculture
E)National Institutes of Health
9)Following successful completion of a phase III trial for a particular drug, a biotechnology company would
apply for a(an)________ to receive approval to sell the drug.
A)DNA
B)NDA
C)Copyright
D)Patent
E)IND
10)The ________ is the federal agency that provides a majority of federal funding for medical research in
the United States and oversees all federally funded medical research in the United States.
A)Department of Environmental Protection
B)Food and Drug Administration
C)Environmental Protection Agency
D)U.S. Department of Agriculture
E)National Institutes of Health
11)An average time range necessary for completing phase testing of drugs during clinical trials is
approximately:
A)6 months to 1 year
B)1 to 3 years
C)2 to 4 years
D)5 to 8 years
E)8 to 12 years
12)A ________ provides an inventor with government-granted protection of an invention for a period of
time, preventing others from using or making the invention without being licensed to do so.
A)Licensing agreement
B)Patent
C)Royalty
D)NDA
E)Clinical trial
13)Phase ________ trials occur when a drug is tested on individuals with a disease to determine the
effectiveness of a drug in treating a disease.
A)I
B)II
C)III
D)IV
E)V
CHP 11
1)Which of the following is an example of in vivo gene therapy?
A)Using retroviruses to deliver therapeutic genes directly into a patient
B)Injecting stem cells into a patient
C)Pharmacogenomics
D)Using retroviruses to deliver therapeutic genes into cultured cells
E)None of these choices
2)________ is the use of genes for treating human genetic disorders.
A)Recombinant DNA technology
B)Genetics
C)Genomics
D)Pharmacogenomics
E)Gene therapy
3)What types of cells fuse together to form hybridomas for making monoclonal antibodies?
A)Monoclonal antibodies and myeloma cells
B)Liver cells and antibody-forming cells
C)Myeloma cells and B cells
D)Bacterial cells and T cells
E)None of these choices
4)Which state passed Proposition 71, an initiative to use $3 billion in taxpayer's money to fund embryonic
stem cell research?
A)Massachusetts
B)New York
C)Wisconsin
D)California
E)New Jersey
5) Which of the following genetic testing techniques would be the best choice for detecting a chromosome
translocation?
A)RFLP analysis
B)Microarray analysis
C)ASO test
D)FISH
E)VNTR analysis
6)Which of the following techniques would be the best choice for screening a person's genetic profile for
1,000 or more genes?
A)Microarray analysis
B)VNTR analysis
C)ASO test
D)FISH
E)RFLP analysis
7)Which of the following is the source of embryonic stem cells?
A)Inner cell mass
B)Trophoblast
C)Spermatozoa
D)Enucleated egg cells
E)Blastocoel
8)The first human genetic disease to be successfully treated by gene therapy was:
A)Albinism
B)Cystic fibrosis
C)Chronic myelogenous leukemia
D)Adenosine deaminase deficiency
E)Sickle cell anemia
9)Which of the following genetic disorders is created by a translocation between chromosome 9 and
chromosome 22?
A)Chronic myelogenous leukemia
B)Cystic fibrosis
C)Adenosine deaminase deficiency
D)Sickle cell anemia
E)None of these choices
10)A limitation of retrovirus-mediated gene transfer is:
A)Retroviruses integrate DNA randomly in the genome
B)Retroviruses can only be used to make transgenic plants
C)Retroviruses only work with Ti vectors
D)Retroviruses only integrate DNA at specific sites
E)Retroviruses cannot be used to make transgenic animals
11)Which of the following techniques would be used to prepare a fetal karyotype to detect trisomy 21
(Down syndrome)?
A)STR analysis
B)RFLP analysis
C)Chorionic villus sampling
D)Microarray analysis
E)ASO test
12)All of the following phrases about embryonic stem cells are true except:
A)Self-renewing
B)Can differentiate into all body cells
C)Derived from the inner cell mass of a blastocyst
D)Pluripotent
E)Are present in all adult tissues
13)All of the following statements about telomeres and telomerase are true except:
A)Telomerase minimizes telomere shortening
B)Telomerase activity is high in adult cells
C)Telomeres shorten as cells age (senescence)
D)Telomerase activity is high in embryonic stem cells
E)None of these choices
14)Transplanting a tissue or organ from one species into another species is known as:
A)Autotransplantation
B)Transgenesis
C)Regenerative medicine
D)Transformation
E)Xenotransplantation
15)Which of the following best describes RNAi techniques for gene silencing?
A)siRNAs inhibit transcription of a gene
CHP 10
1)Known for their great strength and adhesive properties, components of byssal fibers produced by
________ are being studying for a variety of applications from medical sutures to adhesives.
A)Crassotrea virginica
B)Pfiesteria piscicida
C)Vibrio cholera
D)Conus magus
E)Mytilus edulis
2)Polyploid organisms:
A)Have different numbers of each chromosome
B)Are created from ES cells
C)Are transgenic
D)Are knockout organisms
E)Have extra complete sets of chromosomes
3)The gene for green fluorescent protein (GFP), a commonly used reporter gene, was cloned from:
A)Vibrio cholerae
B)Aequorea victoria
C)Sus domesticus
D)Danio rerio
E)Pfiesteria piscicida
4)Growing aquatic plants and finfish species together with shared water resources is an example of:
________.
A)Polyploidy
B)Polyculture
C)Hydroponics
D)Fish farming
E)None of these choices
5)Known as the "cell from hell," this toxin-producing dinoflagellate is responsible for significant fish kills in
the mid-Atlantic region.
A)Mytilus edulis
B)Vibrio cholera
C)Crassotrea virginica
D)Pfiesteria piscicida
E)Conus magus
6)Which of the following is a pigment used to alter the color of salmon?
A)Green fluorescent protein
B)Auxin
C)Hydroxyapatite
D)AFP
E)Astaxanthin
7)Transgenic salmon engineered to grow faster than nontransgenic strains contain extra copies of the
________ gene.
A)AFP
B)GFP
C)Calcitonin
D)GH
E)HA
8)Marine cone snails, Conus magus, are the source of:
A)Calcitonin used to treat osteoporosis
B)Cells for the LAL test used to detect bacterial contamination of surgical tools
C)Prialt, a peptide used as a pain reliever
D)Growth hormone used to treat dwarfism
E)Hydroxyapatite used to treat arthritis
CHP 9
1)Which bacterium is capable of withstanding doses of radiation that are several times higher than human
cells can tolerate?
A)Staphylococcus aureus
B)Deinococcus radiodurans
C)Escherichia coli
D)Saccharomyces cerevisiae
E)Conus magus
2)Which is not an indigenous microbe used for bioremediation?
A)Piscirikettsis salmonis
B)E. coli
C)Phanerochaete sordida
D)Pseudomonas aeruginosa
E)Deinococcus radiodurans
3)Ananda Chakrabarty received the first U.S. patent for a GM organism. This organism was:
A)A transgenic mouse expressing the growth hormone gene
B)Dolly the cloned sheep
C)Cloned E. coli
D)The GloFish
E)Pseudomonas engineered to degrade petroleum
4)During which stage of wastewater treatment are methanogenic microbes most important?
A)Primary treatment B)Sludge digestion C)Biological oxidation D)Secondary treatment E)Disinfection
5)Anaerobic bacteria often play important roles in bioremediation. Which of the following is not an electron
acceptor used by anaerobes during biodegradation reactions?
A)CO 2
B)NO 3C)Fe(III)
D)H 2 O
E)SO 4-2
6)Bioaugmentation is a process that involves:
A)Using plants for bioremediation
B)Bioventing
C)Sludge removal
D)Adding microbes to a cleanup site
E)Ex situ bioremediation
7)Which bioremediation approach involves mixing contaminated soil with water, carbon dioxide, and
fertilizers in a bioreactor to stimulate biodegradation?
A)In situ hybridization
B)Slurry-phase bioremediation
C)Biopile treatment
D)In situ bioremediation
E)Composting
8)Which bioremediation approach involves using plants to degrade pollutants?
A)Biopile
B)Phytoremediation
C)Composting
D)Land farming
E)None of these choices
9)During which stage of wastewater treatment is the primary effluent aerated to allow for biodegradation
by aerobic microbes?
A)Sedimentation
B)Secondary treatment
C)Sludge digestion
D)Disinfection
E)Primary treatment
10)Which cleanup approach involves removing groundwater or soil from its natural setting to allow for
bioremediation?
A)In situ bioremediation
B)Ex situ bioremediation
C)Bioaugmentation
D)Phytoremediation
E)None of these choices
11)Known for their great strength and adhesive properties, components of byssal fibers produced by
________ are being studying for a variety of applications from medical sutures to adhesives.
A)Conus magus
B)Pfiesteria piscicida
C)Vibrio cholera
D)Mytilus edulis
E)Crassotrea virginica
12)Known for their great strength and adhesive properties, components of byssal fibers produced by
________ are being studying for a variety of applications from medical sutures to adhesives.
A)Crassotrea virginica
B)Vibrio cholera
C)Pfiesteria piscicida
D)Mytilus edulis
E)Conus magus
13)Polyploid organisms:
A)Are transgenic
B)Are knockout organisms
C)Have different numbers of each chromosome
D)Are created from ES cells
E)Have extra complete sets of chromosomes
14)The gene for green fluorescent protein (GFP), a commonly used reporter gene, was cloned from:
A)Vibrio cholerae
B)Sus domesticus
C)Danio rerio
D)Aequorea victoria
E)Pfiesteria piscicida
15)Growing aquatic plants and finfish species together with shared water resources is an example of:
________.
A)Hydroponics
B)Polyploidy
C)Fish farming
D)Polyculture
E)None of these choices
16)Known as the "cell from hell," this toxin-producing dinoflagellate is responsible for significant fish kills
in the mid-Atlantic region.
A)Conus magus
B)Vibrio cholera
C)Pfiesteria piscicida
D)Crassotrea virginica
E)Mytilus edulis
17)Which of the following is a pigment used to alter the color of salmon?
A)Auxin
B)AFP
C)Hydroxyapatite
D)Astaxanthin
E)Green fluorescent protein
18)Transgenic salmon engineered to grow faster than nontransgenic strains contain extra copies of the
________ gene.
A)HA
B)AFP
C)Calcitonin
D)GH
E)GFP
19)Marine cone snails, Conus magus, are the source of:
A)Hydroxyapatite used to treat arthritis
B)Prialt, a peptide used as a pain reliever
C)Calcitonin used to treat osteoporosis
D)Growth hormone used to treat dwarfism
E)Cells for the LAL test used to detect bacterial contamination of surgical tools
20)Limulus polyphemus are the source of:
A)Calcitonin used to treat osteoporosis
B)Prialt, a peptide used as a pain reliever
C)Cells for the LAL test used to detect bacterial contamination of surgical tools
D)Hydroxyapatite used to treat arthritis
E)Growth hormone used to treat dwarfism
21)Marine ________ is/are a primary source of agar and agarose used in laboratories for making culture
medias and gels.
A)Kelp
B)Mollusks
C)Fish
D)Shrimp
E)Cone snails
22)Which of the following statements is incorrect about triploid species?
A)Are an example of a polyploidy organism
B)Are usually infertile
C)Have an extra copy of one chromosome
D)Have three complete sets of chromosomes
E)All of these choices are correct
23)________ is the farming of a variety of shellfish, crustaceans, finfish, and algae.
A)Organ culture
B)Mariculture
C)Protoplast culture
D)Tissue culture
E)Aquaculture
CHP 8
1)Which scientist is credited with the first example of using DNA fingerprinting evidence to solve a crime?
A)Francis Crick
B)Barry Sheck
C)Alex Jefferies
D)Kary Mullis
E)Colin Pitchfork
2)The physical location of a gene on a chromosome is a gene's ________.
A)Allele
B)Telomere
C)Locus
D)Genome
E)None of these choices
3)________ are tandemly repeated sequences of DNA also called short tandem repeats.
A)VNTRs
B)Microsatellites
C)Telomeres
D)Centromeres
E)Minisatellites
4)Variations in restriction enzyme cutting patterns of DNA from different individuals are called:
A)VNTRs
B)RFLPs
C)Microsatellites
D)Minisatellites
E)STRs
5)Which of the following techniques is most commonly used to separate DNA molecules by size?
A)Chromatography
B)PCR
C)RFLP
D)DNA sequencing
E)Gel electrophoresis
6)The Innocence Project is an effort to use DNA evidence:
A)To study familial inheritance
B)To convict the accused
C)To exonerate convicted individuals
D)For paternity testing
E)For the identification of remains
7)DNA is present in all of the following human cell types except:
A)Hair
B) Erythrocytes (mature red blood cells)
C)Leukocytes (white blood cells)
D) Skin cells
E)Buccal (cheek) cells
8)Single-stranded DNA molecules that can bind to and be used to detect other DNA molecules are called:
A)Primers
B)STRs
C)RFLPs
D)LINES
E)Probes
9)VNTR analysis involves:
A)Analyzing specific loci for 2 base repeating units usually less than 100 bp in size
B)Analyzing specific loci for 2 to 4 bp repeating units
C)PCR amplification of specific genes
D)Cutting DNA with restriction enzyme and analyzing the banding pattern of fragments
E)None of these choices
10)Which of the following cell type would not be suitable for DNA fingerprinting?
A)Hair
B)Buccal (cheek) cells
C)Erythrocytes (mature red blood cells)
D)Skin cells
E)Leukocytes (white blood cells)
11)The DNA profiling database maintained by the FBI is called:
A)GenBank
B)APHIS
C)The Human Genome Project
D)CODIS
E)Innocence Project
12)The source of most mitochondrial DNA an organism inherits is:
A)The nucleus of the sperm
B)The cytoplasm of the sperm
C)The nucleus of the egg
D)The cytoplasm of the egg
E)None of these choices