BO CO KT QU TH NGHIM

Chun b pha dung mi ha cht v iu kin th nghim

Np nha
Ly 2 gam nha ngm vo 50ml dung dch NaOH nng 1M t 1 n 2 gi
loi b cht hu c. Sau ra NaOH d bng nc ct ri ngm vo HNO3 1M
trong khong 1 ngy m
Np nha vo ct SPE chiu cao lp ct 10mm 2 u c nh bng tm polistiren
ng knh l 0,45m
Tin hnh hot ha ct SPE, ch khng ct b kh
Pha cc dung dch lp thit b
+ Pha dung dch HNO3 1M
Ly 7,0 ml HNO3 nng 65% c d = 1,39 vo bnh ri thm nc ct nh mc
ti 100ml
+ Pha dung dch HNO3 1,5M
Ly 10,5 ml HNO3 nng 65% c d = 1,39 vo bnh ri thm nc ct nh mc
ti 100ml
+ Pha dung dch HNO3 2M
Ly 14 ml HNO3 nng 65% c d = 1,39 vo bnh ri thm nc ct nh mc
ti 100ml
+ Pha dung dch HNO3 2,5M
Ly 17,5 ml HNO3 nng 65% c d = 1,39 vo bnh ri thm nc ct nh mc
ti 100ml
+ Pha dung dch HNO3 3M
Ly 21 ml HNO3 nng 65% c d = 1,39 vo bnh ri thm nc ct nh mc
ti 100ml

Pha dung dch chun Pb

+ Pha dung dch Pb 10ppm t dung dch gc 1000ppm
Ht 1 ml dung dch Pb2+ 1000ppm vo bnh nh mc, them nc ct 2 ln vo ri
nh mc ti 100ml
+ Pha dung dch Pb2+ 0,1ppm t dung dch Pb2+ 10ppm
Ht 1 ml dung dch Pb2+ 10ppm vo bnh nh mc, them nc ct 2 ln vo ri
nh mc ti 100ml
Pha dung dch Pb2+ dng ng chun
Pha dy dung dch Pb2+ c nng ln lt l
0,5ppm, 1ppm, 2ppm, 3ppm, 4ppm, 5ppm, 6ppm, 7ppm, 8ppm.
Chun b mu blank
Giy ch th pH
Dung dch HNO3 0,1M v NaOH 0,1M iu chnh pH.
ng chun

Kho st cc yu t nh hng n kh nng hp th ca nha

1. Kho st nh hng ca pH
Chun b 5 mu gi, mi mu cha 100ml Pb2+ c nng 0,1ppm
iu chnh pH cho 5 mu
Mu 1 c pH = 2, Mu 2 c pH = 4, Mu 3 c pH = 5,
Mu 4 c pH = 6, Mu 5 c pH = 7
Cho dung dch chy qua ct SPE tc 1,5ml/pht
Ra gii bng 5ml dung dch HNO3 nng 2,5M
Ri em o trn my quang ph hp th nguyn t AAS ( my AA-6800).
Lp bng

nh hng ca pH n hiu sut hp th ca nha

pH
Nng u
Abs
Nng sau
Hiu sut
th
Kt lun

Mu 1
2
0,1ppm

Mu 2
4
0,1ppm

Mu 3
5
0,1ppm

Mu 4
6
0,1ppm

Mu 5
7
0,1ppm

2. Kho st nh hng ca tc np mu n hiu sut hp th

Chun b 6 mu gi, mi mu cha 100ml Pb2+ c nng 0,1ppm
iu chnh pH cho 6 mu l pH = 6
Cho dung dch chy qua ct SPE tc dng khc nhau
+ Mu 1 chy vi tc 0,5 ml/pht y l tc chy t nhin
+ Mu 2 chy vi tc 1,0 ml/pht
+ Mu 3 chy vi tc 1,5 ml/pht
+ Mu 4 chy vi tc 2,0 ml/pht
+ Mu 5 chy vi tc 2,5 ml/pht
+ Mu 6 chy vi tc 3,0 ml/pht
Ra gii bng 5ml dung dch HNO3 nng 2,5M
Ri em o trn my quang ph hp th nguyn t AAS ( my AA-6800).
Lp bng
ml/pht
Nng u
Abs
Nng sau
Hiu sut

Mu 1
0,5

Mu 2
1,0

Mu 3
1,5

Mu 4
2,0

Mu 5
2,5

Mu 6
3,0

 th
Kt lun

3. Kho st qu trnh ra gii Pb2+ ra khi ct SPE

Chun b 5 mu gi, mi mu cha 100ml Pb2+ c nng 0,1ppm
iu chnh pH cho 5 mu l pH = 6
Cho dung dch chy qua ct SPE tc dng l 1,5ml/pht
Sau em ra gii bng dung dch HNO3 vi cc nng khc nhau
+ Mu 1 ra gii bng 5ml dung dch HNO3 nng 1,0 M
+ Mu 2 ra gii bng 5ml dung dch HNO3 nng 1,5 M
+ Mu 3 ra gii bng 5ml dung dch HNO3 nng 2,0 M
+ Mu 4 ra gii bng 5ml dung dch HNO3 nng 2,5 M
+ Mu 5 ra gii bng 5ml dung dch HNO3 nng 3,0 M
em mu thu c o trn my o quang ph hp th nguyn t AAS
( my AA 6800) Lp bng
nh hng ca nng axit n hiu sut ra gii
Mu 1

Mu 2

Mu 3

Mu 4

Mu 5

Nng HNO3
Nng u
Abs
Nng sau
Hiu sut
th
Kt lun

0,5M
0,1ppm

1M
0,1ppm

2M
0,1ppm

2,5M
0,1ppm

3M
0,1ppm