Amin-Aminoaxt

PHNG PHP GII BI TON THY PHN PEPTIT

PHN I: Cc dng ton
1. Phn ng thy phn ca Peptit:
a. Thy phn hon ton: theo phng trnh phn ng
H[NHRCO]nOH + (n-1) H2O nH2NRCOOH.
b. Thy phn khng hon ton
Cch gii :
*p dng LBTKL tnh lng nc khi bit khi lng Peptit phn ng v khi lng cht sinh ra.
*p dng LBTKL tnh c lng mui khi cho Aminoacid sinh ra tc dng vi HCl, H2SO4.
* Khi tinh ton nn tnh theo cch 2 trn.
Tnh nhanh khi lng Mol ca Peptit:
H[NHCH2CO]4OH .
Ta c M= MGli x 4 3x18 = 246g/mol
H[NHCH(CH3)CO]3OH
Ta c M= MAla x 3 2x18 = 231g/mol
H[NHCH2CO]nOH .
Ta c M= [MGli x n (n-1).18]g/mol
* i vi 2 Peptit khi thy phn c t l s mol bng nhau,th ta xem 2 Peptit l mt Peptit v ghi phn ng ta nn ghi gp. Khi lng
mol ca Petpti chnh l tng khi lng mol ca 2 Peptit .
V d: Tripeptit H[NHCH2CO]3OH v Tetrapeptit H[NHCH2CO]4OH (c s mol bng nhau) th ta xem 2 Peptit l Heptapeptit:
H[NHCH2CO]7OH v M= 435g/mol
V d:
Bi 1: X l mt Tetrapeptit cu to t Aminoacid A, trong phn t A c 1 nhm(-NH2), 1 nhm (-COOH) ,no, mch h. Trong A Oxi
chim 42,67% khi lng. Thy phn m gam X trong mi trng acid th thu c 28,35(g) tripeptit; 79,2(g) ipeptit v 101,25(g) A.
Gi tr ca m l?
A. 184,5.
B. 258,3.
C. 405,9.
D. 202,95.
Hng dn:
T % khi lng Oxi trong A ta xc nh c A l Gli ( H2NCH2COOH) vi M=75
Cng thc ca Tetrapeptit l H[NHCH2CO]4OH vi M= 75x4 3x18 = 246g/mol
Tnh s mol: Tripeptit l : 28,35: 189 = 0,15(mol)
ipeptit l : 79,2 : 132 = 0,6 (mol)
Glyxin(A) : 101,25 : 75 = 1,35(mol).
Gii gn nh sau: t mt xch NHCH2CO = X
Ghi s phn ng :
(X)4
0,15

(X)3 +
0,15

(X)4
0,3

2 (X)2
0,6

X
0,15

mol
mol

(X)4
4X
0,3
1,2
mol
T s trn ta tnh c: S mol X phn ng l: (0,15+0,3+0,3)=0,75mol
m = 0,75.246 =184,5(g)
Bi 2: Thy phn hon ton 143,45 gam hn hp A gm hai tetrapeptit thu c 159,74 gam hn hp X gm cc Aminoacid (Cc
Aminoacid ch cha 1nhm -COOH v 1 nhm -NH2 ) . Cho tan b X tc dng vi dung dch HCl d,sau c cn dung dch th nhn
c m(gam) mui khan. Tnh khi lng nc phn ng v gi tr ca m ln lt bng?
a. 8,145(g) v 203,78(g). b. 32,58(g) v 10,15(g).
c. 16,2(g) v 203,78(g) d. 16,29(g) v 203,78(g).
Hng dn:
t Cng thc chung cho hn hp A l H[NHRCO]4OH
Ta c phn ng : H[NHRCO]4OH + 3H2O
Hay:

(X)4 + 3H2O

nH2O =

4
n
3

T phn ng

nX=

4X

mH2O = 16,29 gam.

4
.0,905( mol )
3
H2O =

Phn ng ca X tc dng vi HCl :

( Trong X = HNRCO)

mX mA
0,905(mol )

18

p dng LBTKL

4 H2NRCOOH

X + HCl

X.HCl

4
.0,905( mol )
3

p dng BTKL
m(Mui) = mX + mHCl = 159,74 +
.36,5 = 203,78(g)
Bi 3:
Tripeptit M v Tetrapeptit Q c to ra t mt aminoacid X mch h ( phn t ch cha 1 nhm NH2 ). Phn trm khi lng
Nito trong X bng 18,667%. Thy phn khng hon ton m(g) hn hp M,Q(c t l s mol 1:1) trong mi trng Acid thu c
0,945(g) M; 4,62(g) ipeptit v 3,75 (g) X.Gi tr ca m?
A. 4,1945(g).
B. 8,389(g).
C. 12,58(g).
D. 25,167(g).

Amin-Aminoaxt
Hng dn:

14 18,667

MX 75
MX
100
Cch 1: Ta c %N =
X l Glyxin
Do hai peptit c t l s mol phn ng 1:1 nn xem hn hp M,Q l mt Heptapeptit : H[NHCH 2CO]7OH v c M = 435g/mol.

27
7
S phn ng :

(Gli)7 + H2O

(Gli)3

7 (Gli)2

+ 10 (Gli)

27
7
0,005mol

0,005mol

0.035mol

0.05mol

27
7

m(M,Q) =

0,005mol.435 = 8,389(g)

Cch 2
(Gli)7
2(Gli)3 + Gli ; (Gli)7
3 (Gli)2 + Gli v (Gli)7
7(Gli)
0,0025mol
0,005mol 0,0025
0,035/3
0,035mol
0,035/3 0,0358/7
0.0358
T cc phn ng tnh c s mol ca (Gli)7 l : 0.01928(mol)
2. Phn ng chy ca Peptit:
V d: Tripeptit mch h X v Tetrapeptit mch h Y c to t mt Aminoacid no, h trong phn t c 1nhm (-NH2 ) v 1nhm (COOH). t chy X v Y. Vy lm th no t CTPT cho X,Y? Ta lm nh sau:
T CTPT ca Aminoacid no 3 CnH2n+1O2N 2H2O thnh CT C3nH6n 1O4N3(y l cng thcTripeptit) V
4 CnH2n+1O2N
3H2O thnh CT C4nH8n 2O5N4(y l cng thcTetrapeptit) ...... Nu t chy lin quan n lng nc v cacbonic th ta ch cn cn
bng C,H tnh ton cho nhanh.
C3nH6n 1O4N3 + pO2

3nCO2 + (3n-0,5)H2O + N2

C4nH8n 2 O5N4 + pO2

4nCO2 + (4n-1)H2O + N2
Tnh p(O2) dng BT nguyn t Oxi?
Bi 4: Tripeptit mch h X v Tetrapeptit mch h Y u c to ra t mt aminoacid no,mch h c 1 nhm COOH v 1 nhm
NH2 .t chy hon ton 0,1 mol X thu c sn phm gm H2O,CO2 v N2 trong tng khi lng CO2 v H2O bng 36,3(g) .Nu t
chy hon ton 0,2 mol Y th s mol O2 cn phn ng l?
a. 2,8(mol).
b. 1,8(mol).
c. 1,875(mol).
d. 3,375 (mol)
Hng dn:
R rng X,Y u sinh ra do Aminoacid c CT CnH2n+1O2N.
Do vy ta c CT ca X,Y tng ng l: C3nH6n 1O4N3(X) , C4nH8n 2O5N4(Y).
Phn ng chy X: C3nH6n 1O4N3 +

pO2
0,1mol

3nCO2 + (3n-0,5)H2O + N2
0,3n(mol) 0,3(3n-0,5)mol

Ta c phng trnh tng khi lng H2O v CO2 : 0,3[44.n + 18. (3n-0,5)] = 36.3
Phn ng chy Y: C4nH8n 2 O5N4 + pO2
0,2mol
p dng BT nguyn t Oxi :

0,2.p

n=2

4nCO2 + (4n-1)H2O + N2 .
0,8n
(0,8n -0,2)

0,2.5+ 0,2.2p = 0,8.2.2 +(0,8.2 -0,2)

p = 9.
nO2 = 9x0,2 = 1,8(mol)
PHN II:
BI TP P DNG
Bi 1: Thy phn hon ton 60(g) hn hp hai ipeptit thu c 63,6(g) hn hp X gm cc Aminoacid no mch h (H2NRCOOOH).
Nu ly 1/10 hn hp X tc dng vi dung dch HCl d thu c m(g) mui. Gi tr ca m l?
a. 7,82.
b. 8,72. c. 7,09.
d.16,3.
Bi 2: Thy phn ht m(g) Tetrapeptit Ala-Ala-Ala-Ala thu c hn hp gm 28,48(g) Ala ; 32(g) Ala-Ala v 27,72(g) Ala-Ala-Ala.
Gi tr ca m?
a. 66,44.
b. 111,74.
c. 81,54.
d. 90,6.
Bi 3: X l mt Hexapeptit cu to t mt Aminoacid H2N-CnH2n-COOH(Y). Y c tng % khi lng Oxi v Nito l 61,33%. Thy
phn ht m(g) X trong mi trng acid thu c 30,3(g) pentapeptit, 19,8(g) ieptit v 37,5(g) Y. Gi tr ca m l?
a. 69 gam.
B. 84 gam.
c. 100 gam.
d.78 gam.
Bi 4: X l mt tetrapeptit cu to t mt amino axit (A) no, mch h c 1 nhm COOH ; 1 nhm NH2. Trong A %N = 15,73% (v
khi lng). Thy phn m gam X trong mi trng axit thu c 41,58 gam tripeptit ; 25,6 gam ipeptit v 92,56 gam A. Gi tr ca m
l : a. 149 gam.
b. 161 gam.
c. 143,45 gam.
d. 159 gam.
Bi 5: X l tetrapeptit Ala-Gly-Val-Ala, Y l tripeptit Val-Gly-Val. un nng m gam hn hp X v Y c t l s mol nX : nY = 1 : 3 vi
780 ml dung dch NaOH 1M (va ), sau khi phn ng kt thc thu c dung dch Z. C cn dung dch thu c 94,98 gam mui. m
c gi tr l a.. 68,1 gam.
b. 64,86 gam.
c. 77,04 gam.
d. 65,13 gam.
Bi 6 ipeptit mch h X v Tripeptit mch h Y u c to ra t mt Aminoacid no,mch h c 1 nhm COOH v 1 nhm NH2
.t chy hon ton 0,1 mol Y thu c sn phm gm H2O,CO2 v N2 trong tng khi lng CO2 v H2O bng 54,9(g) .Nu t chy
hon ton 0,2 mol X,sn phm thu c cho li qua dung dch nc vi trong d th c m(g) kt ta . Gi tr ca m l?
a. 45.
b. 120.
c.30.
d.60.

Amin-Aminoaxt
Bi 7: X v Y ln lt l cc tripeptit v tetrapeptit c to thnh t cng mt amino axit no mch h, c mt nhm COOH v mt
nhm NH2. t chy hon ton 0,1 mol Y thu c sn phm gm CO2, H2O, N2, trong tng khi lng ca CO2 v H2O l
47,8 gam. Nu t chy hon ton 0,3 mol X cn bao nhiu mol O2 ?
a. 2,8 mol.
b. 2,025 mol.
c. 3,375 mol.
d. 1,875 mol.

Bi 8:Thy phn 14(g) mt Polipeptit(X) vi hiu sut t 80%,thi thu c 14,04(g) mt - aminoacid (Y). Xc nh Cng thc cu
to ca Y?
a. H2N(CH2)2COOH.b. H2NCH(CH3)COOH.
c. H2NCH2COOHd. H2NCH(C2H5)COOH
Bi 9: un nng alanin thu c mt s peptit trong c peptit A c phn trm khi lng nit l 18,54%. Khi lng phn t ca
A l : a. 231.
b. 160.
c. 373.
d. 302.
Bi 10: Khi thy phn hon ton 55,95 gam mt peptit X thu c 66,75 gam alanin (amino axit duy nht). X l :
a. tripeptit.
b. tetrapeptit.
c. pentapeptit. d. ipeptit.
Bi 11: Khi thy phn hon ton 65 gam mt peptit X thu c 22,25 gam alanin v 56,25 gam glyxin. X l :
a. tripeptthu c.
b. tetrapeptit.
c. pentapeptit. d. ipeptit.
Bi 12: Thu phn hon ton 500 gam mt oligopeptit X (cha t 2 n 10 gc -amino axit) thu c 178 gam amino axit Y v 412
gam amino axit Z. Bit phn t khi ca Y l 89. Phn t khi ca Z l :
a. 103.
b. 75.
c. 117.
d. 147.
Bi 13: Tripeptit X c cng thc sau :
H2NCH2CONHCH(CH3)CONHCH(CH3)COOH
Thy phn hon ton 0,1 mol X trong 400 ml dung dch NaOH 1M. Khi lng cht rn thu c khi c cn dung dch sau phn ng l :
a. 28,6 gam.
b. 22,2 gam.
c. 35,9 gam.
d. 31,9 gam.
Bi 14: Protein A c khi lng phn t l 50000 vC. Thu phn 100 gam A thu c 33,998 gam alanin. S mt xch alanin trong
phn t A l :
a. 191.
b. 38,2.
c. 2.3.1023 d. 561,8.
Bi 15: Thy phn 1250 gam protein X thu c 425 gam alanin. Nu phn t khi ca X bng 100000 vC th s mt xch alanin c
trong X l : a. 453.
b. 382.
c. 328.
d. 479.
Bi 16:Xc nh Phn t khi gn ng ca mt Polipeptit cha 0,32% S trong phn t. Gi s trong phn t ch c 2 nguyn t S?
a. 20.000(vC)
b.10.000(vC). c. 15.000(vC). d. 45.000(vC).
Bi 17: Mt hemoglobin (hng cu ca mu) cha 0,4% Fe (mi phn t hemoglobin ch cha 1 nguyn t Fe). Phn t khi gn ng
ca hemoglobin trn l : a. 12000.
b. 14000.
c. 15000.
d. 18000.
Bi 18: Thy phn hon ton 1 mol Pentapeptit(X) thu c 3 mol Gli; 1 mol Ala; 1 mol Phe. Khi thy phn khng hon ton (X) thu
c hn hp gm Ala-Gli ; Gli-Ala v khng thy to ra Phe-Gli. Xc nh CTCT ca Petapeptit?
Hng dn: T cc ipeptit ta thy Gli gia Ala-Gli-Ala hoc Ala gia Gli-Ala-Gli. Nhng v thu c 1 mol Ala nn chc
chn Ala phi gia Gli-Ala-Gli. Do khng c Phe-Gli to thnh nn Phe khng ng trc Gli m ng sau Gli.
Vy CTCT l: Gli-Gli-Ala-Gli-Phe
Bi 19: Thu phn hon ton 1 mol pentapeptit A th thu c 3 mol glyxin ; 1 mol alanin v 1mol valin. Khi thu phn khng hon
ton A th trong hn hp sn phm thy c cc ipeptit Ala-Gly ; Gly-Ala v tripeptit Gly-Gly-Val. Amino axit u N, amino axit u C
pentapeptit A ln lt l :
a. Gly, Val.
b. Ala, Val.
c. Gly, Gly. d. Ala, Gly.
Bi 20: Thu phn khng hon ton tetrapeptit (X), ngoi cc a-amino axit cn thu c cc ipetit: Gly-Ala ; Phe-Val ; AlaPhe. Cu to no sau y l ng ca X ?
a. Val-Phe-Gly-Ala.
b. Ala-Val-Phe-Gly. c. Gly-Ala-Val-Phe.
d. Gly-Ala-Phe-Val.
Bi 21: Thy phn hon ton 1 mol pentapeptit X, thu c 2 mol glyxin (Gly), 1 mol alanin (Ala), 1 mol valin (Val) v 1 mol
phenylalanin (Phe). Thy phn khng hon ton X thu c ipeptit Val-Phe v tripeptit Gly-Ala-Val nhng khng thu c ipeptit
Gly-Gly. ht X c cng thc l
a. Gly-Phe-Gly-Ala-Val.
b. Gly-Ala-Val-Val-Phe. c. Gly-Ala-Val-Phe-Gly.
d. Val-Phe-Gly-Ala-Gly.
Bi 22: Cng thc no sau y ca pentapeptit (A) tha iu kin sau ?
hy phn hon ton 1 mol A th thu c cc - amino axit l : 3 mol glyxin, 1 mol alanin, 1 mol valin. Thy phn khng hon ton A,
ngoi thu c cc amino axit th cn thu c 2 ipeptit : Ala-Gly ; Gly-Ala v 1 tripeptit Gly-Gly-Val.
a. Ala-Gly-Gly-Gly-Val.
b. Gly- Gly-Ala-Gly-Val. c. Gly-Ala-Gly-Gly-Val.
d. Gly-Ala-Gly-Val-Gly.
Bi 23: Thu phn hp cht :
H2NCH2CONHCH(CH3)CONHCH(CH(CH3)2)CONHCH2CONHCH2COOH s thu c bao nhiu loi amino axit no
sau y ?
a. 3.
b. 4.
c. 5.
d. 2.
Bi 24: Thu phn hp cht : s thu c bao nhiu loi amino axit no sau y ?
H2NCH(CH3)CONHCH(CH(CH3)2)CONHCH(C2H5)CONHCH2CONHCH(C4H9)COOH.
a. 2.
b. 3.
c. 4.
d. 5.
Bi 25: Cho 3 cht X,Y,Z vo 3 ng nghim cha sn Cu(OH)2 trong NaOH lc u v quan st th thy: Cht X thy xut hin mu
tm, cht Y th Cu(OH)2 tan v c mu xanh nht, cht Z th Cu(OH)2 tan v c mu xanh thm. X, Y, Z ln lt l :
a. H tinh bt, HCOOH, mantoz.
b. Protein, CH3CHO, saccaroz.
c. Anbumin, C2H5COOH, glyxin.
d. Lng trng trng, CH3COOH, glucoz.
Bi 26: Thuc th c dng phn bit Gly-Ala-Gly v Gly-Ala l :
a. dd HCl.
b. Cu(OH)2/OH- c. dd NaCl.
d. dd NaOH.
Cu 1: Khi thy phn 500 g protein A thu c 170 g alanin. Nu phn t khi ca A l 50000 vC th s mt xch alanin trong phn t
ca A l
A. 190. B. 191. C. 192.
D. 193.
Cu 2: Cng thc no sau y ca pentapeptit (A) tha iu kin sau:

+ Thy phn hon ton 1 mol A th thu c cc - amino axit l: 3 mol Glyxin , 1 mol Alanin, 1 molValin.
+ Thy phn khng hon ton A, ngoi thu c cc amino axit th cn thu c 2 i peptit: Ala-Gly ; Gly- Ala vGly-Gly-Val.
A. Ala-Gly-Gly-Gly-Val.
B. Gly-Gly-Ala-Gly-Val.
C. Gly-Ala-Gly-Gly-Val.
D. Gly-Ala-Gly-Val-Gly.

Amin-Aminoaxt

Cu 3: Thu phn khng hon ton tetra peptit (X), ngoi cc - amino axit cn thu c cc i petit: Gly-Ala; Phe-Val; Ala-Phe. Cu
to no sau y l ng ca X.
A. Val-Phe-Gly-Ala.
B. Ala-Val-Phe-Gly.
C. Gly-Ala-Val-Phe
D. Gly-Ala-Phe Val.
Cu 7: Ly 14,6g mt ipeptit to ra t glixin v alanin cho tc dng va vi dung dch HCl 1M. Th tch dung dch HCl tham gia
phn ng:
A. 0,1 lit
B. 0,2 lt
C. 0,3 lt
D. 0,4 lt
Cu 8 : Thu phn 1250 gam protein X thu c 425 gam alanin. Nu phn t khi ca X bng 100.000 vC th s mt xch alanin c
trong phn t X l
A. 328.
B. 453.
C. 479.
D. 382.
Cu 15: tn gi cho peptit sau:
H2N-CH-CO-NH-CH2-CO-NH-CH-COOH
CH3
CH3
A. alanylglyxylalanyl.
B. glixylalanylglyxin.
C. glixylalanylglyxin. D. alanylglixylalanin.
Cu 25: Nu thu phn khng hon ton pentapeptit Gly-Ala-Gly-Ala-Gly th thu c ti a bao nhiu ipeptit khc nhau? A. 4.
B. 2.
C. 3.
D. 1.
Cu 30: Thu phn hon ton 1 mol pentapeptit A th thu c 3 mol glyxin; 1 mol alanin v 1mol valin. Khi thu phn khng hon ton
A th trong hn hp sn phm thy c cc ipeptit Ala-Gly; Gly-Ala v tripeptit Gly-Gly-Val. Aminoaxit u N, amino axit u C
pentapeptit A ln lt l A. Gly, Val.
B. Ala, Val.
C. Gly, Gly.
D. Ala, Gly.
Cu 35: Ly 8,76 g mt ipeptit to ra t glyxin v alanin cho tc dng va vi dung dch HCl 1M. Th tch dung dch HCl tham gia
phn ng l:
A. 0,1 lt
B. 0,06 lt
C. 0,24 lt
D. 0,12
Cu 14: ipeptit mch h X v tripeptit mch h Y u c to nn t mt aminoaxit (no, mch h, trong phn t cha mt nhm
-NH v mt nhm -COOH). t chy hon ton 0,1 mol Y, thu c tng khi lng CO v H O bng 54,9 gam. t chy hon ton
2
2
2
0,2 mol X, sn phm thu c cho li t t qua nc vi trong d, to ra m gam kt ta. Gi tr ca m l
A. 120. B. 45. C. 60. D. 30.
Cau 10. Cong thc nao sau ay cua pentapeptit (A) thoa ieu kien sau:
+ Thuy phan hoan toan 1 mol A th thu c cac - amino axit la: 3 mol Glyxin , 1 mol Alanin, 1
mol Valin.
+ Thuy phan khong hoan toan A, ngoai thu c cac amino axit th con thu c 2 i peptit:
Ala-Gly va Gly- Ala va mot tri peptit Gly-Gly-Val.
A. Ala-Gly-Gly-Gly-Val.
B. Gly-Gly-Ala-Gly-Val.
C. Gly-Ala-Gly-Gly-Val.
D.
GlyAla-Gly-Val-Gly.
Cau 11. Thuy phan khong hoan toan tetra peptit (X), ngoai cac - amino axit con thu c cac
i petit: Gly-Ala; Phe-Va; Ala-Phe. Cau tao nao sau ay la ung cua X.
A. Val-Phe-Gly-Ala.
B. Ala-Val-Phe-Gly.
C. Gly-Ala-Val-Phe
D. Gly-Ala-Phe
Val.
Cau 17. Bradikinin co tac dung lam giam huyet ap, o la mot nonapeptit co cong thc la:
Arg Pro Pro Gly-Phe-Ser-Pro-Phe-Arg.
Khi thuy phan khong hoan toan peptit nay co the thu c bao nhieu tri peptit ma thanh
phan co cha phenyl alanin ( phe).
A.3
B.4
C.5
D.6