ABBOTT CELL-DYN 1800 Hematology Analyzer Manual

ABBOTT
CELL-DYN 1800
Automated Hematology Analyzer
Service & Support Manual
ABBOTT CD1800 SM
Theory of Operation ............................................................................................................................................................................ 4
System Overview.................................................................................................................................................................... 4
Major Subsystem Descriptions........................................................................................................................................... 6
Circuit Descriptions ............................................................................................................................................................. 15
Signal Processor Module (SPM)....................................................................................................................................... 18
Cell Count Module (CCM) ................................................................................................................................................... 21
Troubleshooting................................................................................................................................................................................. 31
Troubleshooting Charts...................................................................................................................................................... 33
Raw Data Description.......................................................................................................................................................... 40
CCM On-Board Diagnostic LEDs...................................................................................................................................... 41
CPU Hardware/Software Configuration .......................................................................................................................... 44
Service Special Commands............................................................................................................................................... 45
Sample Probe Description ................................................................................................................................................. 49
CELL-DYN 1800 Error Messages...................................................................................................................................... 60
Software Commands and Sequence ............................................................................................................................... 72
Engineering Drawings and Schematics ............................................................................................................................................. 76
CELL-DYN 1800 PCB Reference .................................................................................................................................................... 77
Removal & Replacement .................................................................................................................................................................. 96
Service Equipment Required............................................................................................................................................. 96
Covers (CD18-A1) .................................................................................................................................................................... 97
Flow Panel (CD18-B1)............................................................................................................................................................ 105
Fluid Power Supply (CD18-C1).............................................................................................................................................. 115
Syringe Assembly (CD18-E1)................................................................................................................................................. 119
RR-E1.04 Sample Syringe Driver Assembly .................................................................................................................... 125
RR-E1.06 Lyse Syringe Driver Assembly..................................................................................................................... 127
Electronics / Card Cage (CD18-F1) ........................................................................................................................................ 129
RR-F1.01 PAM (Pre-Amplifier Module) ............................................................................................................................. 129
RR-F1.02 MPM (Motor Processor Module) Board .......................................................................................................... 131
RR-F1.03 CDM (Cable Distribution Module) Board ........................................................................................................ 133
RR-F1.04 Hard Disk Drive................................................................................................................................................... 135
RR-F1.05 Floppy Disk Drive ............................................................................................................................................... 138
RR-F1.06 Signal Processor Module (SPM)...................................................................................................................... 140
RR-F1.07 Cell Count Module (CCM)................................................................................................................................. 142
RR-F1.08 Data Link Adapter (DLA) ................................................................................................................................... 143
RR-F1.09 Single Board Computer (SBC) ......................................................................................................................... 145
RR-F1.10 Card Cage Backplane PCB .............................................................................................................................. 147
LCD/Keyboard (CD18-G1)..................................................................................................................................................... 149
Power Supply (CD18-H1).................................................................................................................................................... 151
Verification Procedures ................................................................................................................................................................... 154
VP-01 Preparation for Alignment and Verification ................................................................................................................. 154
VP-01 Preparation for Alignment and Verification..................................................................................................... 154
Test Equipment and Supplies Required....................................................................................................................... 154
Preparation for Alignment/Verification ......................................................................................................................... 155
Order of Alignment/Verification...................................................................................................................................... 156

VP-02 Decontamination .................................................................................................................................................... 157
VP-03 Vacuum and Pressure Adjustments.................................................................................................................. 158
Regulator Alignment.......................................................................................................................................................... 159
Pressure Adjustment (0.5 psi)......................................................................................................................................... 160
Pressure Verification (High) ............................................................................................................................................ 161
Vacuum Adjustment (8 inch) ........................................................................................................................................... 161
VP-04 Metering System Timing Adjustments - RBC and WBC .............................................................................. 162
Metering Timing Fault Report.......................................................................................................................................... 163
RBC Metering System Timing Adjustment .................................................................................................................. 165
WBC Metering System Timing Adjustment ................................................................................................................. 166
VP-05 CMOS Setup Verification/Adjustment............................................................................................................... 167
VP-06 Card Cage Backplane Test Points ..................................................................................................................... 171
VP-07 Cable Distribution Module Test Points............................................................................................................. 172
VP-08 Pre-Amplifier Module (PAM) Adjustment ......................................................................................................... 173
VP-09 Signal Processor Module (SPM) Verification/Adjustment ........................................................................... 176
RBC Gain .............................................................................................................................................................................. 178
RER Adjustment ................................................................................................................................................................. 179
WBC Gain ............................................................................................................................................................................. 182
PLT Gain ............................................................................................................................................................................... 184
VP-10 Diluent and Sample Verification/Adjustment ........................................................................................................ 187
Diluent Volume Verification ............................................................................................................................................. 188
Sample Volume Verification ............................................................................................................................................ 188
VP-11 Stepper Motor Power Test and Verification .................................................................................................... 189
VP-12 Sample Probe Alignment Check ........................................................................................................................ 190
VP-13 Serial Transmit to LIS Verification..................................................................................................................... 191
VP-14 Hard Disk Drive Setup and Verification............................................................................................................ 194
VP-15 Software Installation/Upgrades .......................................................................................................................... 197
Theory of Operation
System Overview
The CELL-DYN 1800 Automated Hematology Analyzer is a complex system. Analyzer performance depends on several
components that together make up the complete hematology system. The system is comprised of the following components
and subsystems:
Flow Panel System [3]
Fluid Power Supply [2]
Reagent Inlet Panel [6]
Syringe Drive Assemblies [7]
Electronics Card Cage [8]
LCD Display System [4]
Touchpad (Membrane) Keyboard [5]
Power Supply Assembly [1]
Flow Panel
The Flow Panel consists of tubing, solenoid valves, and other hardware components used for sample aspiration, dilution,
measurement and waste removal.
Fluid Power Supply
The Fluid Power Supply contains the vacuum and pressure pumps, accumulators, waste bottles, and associated solenoids
and hardware.
Reagent Inlet Panel
The Reagent Inlet Panel provides connections for incoming reagents and outgoing waste. The Lyse solenoid is also mounted
on this panel.
Syringe Drive Assembies
The Syringe Drive Assemblies include the Sample Syringe for aspirating samples, the Diluent Syringe for supplying Diluent
throughout the Flow Panel, and the Lyse Syringe for dispensing Lyse to the WBC transducer for the HGB measurement
process.
Electronics Card Cage
The electronics card cage, with associated PCBs, provides command and control signals for the various electronic
components of the instrument. This assembly contains the Backplane PCB, Cell Count Module, Signal Processor Module,
Data Link Adaptor and Single Board Computer.
LCD Display Screen and Keyboard
The LCD Display Screen provides a visual data display and the keyboard provides data input by the operator.
Power Supply Assembly
The Power Supply Assembly provides an AC and DC voltage source to various components on the CELL-DYN 1800 System.
The DC Regulator PCB provides power to the Backplane PCB for use with the digital circuitry on various PCBs. It also
provides power to the Cable Distribution Module and fans.
The AC Regulator PCB provides power to the Backplane PCB (for use with the analog circuitry on various PCBs). It also
provides power to the Pump Relay Module.
The +28VDC Switching Power Supply provides power for the stepper motors via the Motor Processor Module. It also
provides power to initially energize solenoids.
Major Subsystem Descriptions
To aid in understanding the overall system, the electronic modules are divided into the following major functional
subsystems:
Data Interface and Control Subsystem
Measurement Subsystem
Solenoid Motor and Pump Subsystem
Single Board Computer Subsystem
AC and DC Power Distribution Subsystem
Data Interface and Control Subsystem

The purpose of this subsystem is to interface the user data, control data, measurement data, and system status data in the
system. This data is connected via four independent data busses:
DLA/CCM (Data Link Adapter/Cell Count Module)
CCM/SPM (Cell Count Module/Sample Processor Module)
CCM/CDM (Cell Count Module/Cable Distribution Module)
CCM/MPM (Cell Count Module/Motor Processor Module)
See the figure below for a diagram showing the data connections.
Data Interface and Control Block Diagram
When power to the instrument is turned ON, the system is operating software is loaded from the hard disk into RAM on the
SBC (Single Board Computer). The SBC then uses various handshaking signals and data bytes to communicate with the
CCM (Cell Count Module) via the DLA (Data Link Adapter).
The CCM functions as the master controller with all system functional commands residing in firmware (PROM). The CCM
sends control data and receives status data from the CDM (Cable Distribution Module).
The CCM provides current control to the von Behrens RBC and WBC Transducers and the two metering PCBs and serves as
the system's analog voltmeter for use in converting the HGB signal. Data is written and read via the CCM/CDM and
CCM/MPM data buses.
The CDM (Cable Distribution Module) acts as a controller for the solenoids and also interfaces data from various system
sensors.
The MPM (Motor Processor Module) acts as a controller for all Stepper Motor Drive PCBs.
Measurement Subsystem
The measurement subsystem provides detection, amplification, and processing of the signals from the von Behrens
RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/PLT and WBC metering is also included in
this subsystem.
See the figure below for a diagram of the measurement process.
Measurement Block Diagram
The PAM (Pre-Amplifier Module) supplies constant current for the von Behrens RBC/PLT and WBC Transducers and HGB
LED voltage.
The RBC/PLT and WBC cell pulses are input to the PAM where they are amplified and routed to the SPM (Signal Processor
Module).
When the SPM receives signals from the RBC/PLT and WBC, the following occurs:
The RBC/PLT signal is amplified (gain) and split into independent RBC and PLT signals.
The WBC signal is amplified (gain).
The RBC signal is routed to the cell editing circuitry.
Cell editing is performed on the RBC signal to eliminate invalid RBC pulses.
The SPM discriminates cell size by converting pulse height to a proportional digital value. The amplitude of each valid pulse
is measured by a fast A/D converter then sent across the data bus to the CCM.
The A/D data for RBC, PLT, and WBC are individually divided by the CCM into 256 discrete size channels. The cell count in
each channel is accumulated in discrete memory locations and is used to generate count data, percentage data, and
histogram data for RBC, PLT, WBC, and other derived parameters.
Signals from the upper and lower detectors on the RBC/PLT and WBC metering PCBs are converted to TTL levels by
comparators on the CDM. The signals are then routed to the CCM where they are used to control RBC/PLT and WBC
sample timing.
The HGB analog signal from the HGB Flow Cell is captured by the PAM where it is amplified and routed to the CCM. The
HGB signal is then measured and converted to a digital format by a voltmeter-A/D converter.
Solenoid, Motor Drive, and Pump Subsystem
Solenoid control commands reside in firmware on the CCM. These commands are sent to the CDM where they are
multiplexed to the appropriate SDM (Solenoid Drive Module). The SDM then provides the current to open and close
individual drive solenoids.
Stepper Motor commands are handled in much the same manner as described above. However, the final multiplexing of the
Stepper Drive PCBs is controlled by the MPM.
There are two pressure pumps and one vacuum pump in the CELL-DYN 1800 System. These pumps are described as
follows:
A pressure pump provides air to bubble-mix samples in the Pre-Mixing Cup and the mixing chambers of the von
Behrens RBC/PLT and WBC Transducers. A pressure regulator regulates the 0.5 psi in the pressure accumulator for
this process.
An unregulated pressure pump provides air to push waste from the waste bottles inside the instrument to the waste
container attached to the instrument and to apply back pressure to clear the apertures in the von Behrens RBC/PLT
and WBC Transducers.
An 8" Hg vacuum accumulator, vacuum sensor, and vacuum pump supply a constant vacuum to the entire system to
transport Diluent, Detergent, and Lyse throughout the flow system and to maintain a constant vacuum to the
RBC/PLT and WBC metering tubes. A vacuum regulator maintains a constant vacuum source to both metering
tubes.
See Solenoid, Motor Drive and Pump Block Diagram for a diagram of the solenoid and motor drive connections.
Solenoid, Motor Drive and Pump Block Diagram
Single Board Computer Subsystem
The Single Board Computer subsystem consists of the following components:
Single Board Computer PCB
Data Link Adapter
Input/Output Ports (serial/parallel)
Keyboards (PC and membrane)
LCD Display Screen
Disk Drives (hard and floppy)
The figure below illustrates the major components of the User Interface Computer.
User Interface Computer
SBC (Single Board Computer) PCB

The Single Board Computer (SBC) PCB is a complete Celeron 850 Megahertz PC computer system with 128 Megabytes of
RAM that is self-contained on one board. It utilizes a PC compatible BIOS with DOS capability that directly interfaces with the
LCD Display Screen, Hard Disk Drive, Floppy Disk Drive, PS/2 Keyboard, one (1) Parallel Port and two (2) Serial Ports. It
connects directly into the Backplane PCB along with the Data Link Adapter (DLA) and utilizes the ISA bus and Backplane
PCB to communicate with one another.
DLA (Data Link Adapter)
The DLA PCB connects directly into the Backplane PCB. It provides a program-controlled data channel from the SBC to the
CCM. The DLA performs the following three functions:
Receives measurement and analyzer status data from the CCM
Transfers commands from the SBC to the CCM
Sends control data from the UIC to the CCM.
Serial I/O Ports

The CELL-DYN 1800 contains two (2) Serial I/O Ports for transferring data to other computer systems:
COM1 RS-232 Data Output

COM2 Spare
Parallel I/O Port

The CELL-DYN 1800 contains one parallel I/O port for transferring data to a printer.
PC/2 Keyboard
The external 101 key PC keyboard is used to enter alphanumeric data, such as demographic information, into the instrument.
Touch Pad (Membrane) Keyboard
The touch pad (membrane) keyboard is located below the LCD Display Screen. The keyboard includes a row of eight (8)
unmarked rectangular-shaped keys corresponding to labels displayed at the bottom of the screen. These keys activate the
indicated function or display the indicated submenu.
LCD Display Screen (Color)
The LCD Display Screen has the following characteristics:
Size: 8.5 x 6.4 inches (10.4 inches measured diagonally)
Number of colors: 16
Pixels: 640 width x 480 height (or 800 x 600)
Backlight on/off control (software controlled screen saver)
An LCD Adapter, connected directly on the SBC PCB, drives the LCD Display Screen. The adapter supports SVGA 640 x
480 and 800 x 600 graphics modes.
Hard Disk Drive
The hard disk drive stores the User Interface Software program and the Patient Data Log.
Floppy Disk Drive

The 1.44MB 3.5" floppy disk drive is used for program installation and provides the QC (Quality Control) data
upload/download capability.
Printer
The User Interface Software supports Epson ESC-P or PCL-3 languages. The printer has its own buffer and is capable of
printing on 8.5" x 11" (letter size) or A4 paper size. The printer supports alphanumeric and graphics reports from stored data
and screen displays.
Speaker
The PC speaker or buzzer is controlled by software and is mounted on the SBC PCB.
AC and DC Power Distribution Subsystem
The Power Supply Assembly is comprised of three components: AC Regulator PCB, DC Regulator PCB and +28VDC
Switching Power Supply. These components are mounted together as an assembly and are located at the right/rear side of
the instrument.
When the system is turned on, the AC line is routed into the AC Regulator PCB and +28VDC Switching Power Supply. The
AC Regulator PCB automatically accommodates line voltages of 90 - 130VAC and 200 - 260VAC by sensing the input
voltage and utilizing an internal comparator bank and power transformer to produce the 120VAC necessary for the
subsystem's function. The Power Supply Assembly then provides an AC and DC voltage source to various components on
the CELL-DYN 1800 System.
+28VDC Switching Power Supply
The +28VDC Switching Power Supply provides the voltage source to the DC Regulator PCB, which the board then uses to
produce other voltages. The power supply cooling fan is thermistor controlled, which means that when the internal
temperature rises above 70C, the fan operates at full speed. The fan is then turned off at 50C and kept off until the
temperature rises above 70C.
AC Regulator PCB
The AC Regulator PCB provides the 120VAC used by the Pump Relay Module (PRM) for vacuum and pressure pump
operation.
The 12VDC (analog) is provided to the Backplane PCB, which is used by the Cell Count Module (CCM) and Signal
Processor Module (SPM). The 12VDC is also provided to the Cable Distribution Module (CDM), Motor Processor Module
(MPM) and Pre-Amplifier Module (PAM) (for its circuitry).
The +100VDC is provided to the PAM for use in its constant current circuitry. In between the AC Regulator PCB and the PAM
is the Pre Amp Filter that is used to filter out noise.
DC Regulator PCB
The DC Regulator PCB provides +5VDC, 12VDC (digital), +14VDC and +28VDC. The +5VDC is provided to the Backplane
PCB, which is used by the SPM, CCM, Data Link Adapter (DLA) and Single Board Computer (SBC). This voltage is also used
by the CDM, MPM, Hard and Floppy Disk Drives.
The 12VDC (digital) is provided to the Backplane PCB for use on the CPU fan. The Hard and Floppy Disk Drives receive
+12VDC for their operation.
The +14VDC is provided through the CDM to the Solenoid Driver Modules (SDM) and is used to hold the solenoids closed or
open for normally closed solenoids.
The +28VDC is provided to the MPM for operating stepper motors and through the CDM to the SDMs to initially close
solenoids or open for normally closed solenoids. This voltage is also used for the system's internal cooling fans. The cooling
fans are thermistor controlled, which means that when the ambient temperature inside the instrument drops below 25C, the
fans operate at half speed. Once the temperature rises above 25C, the speed is increased linearly until it reaches 35C,
when the fans operate at full speed.
The DC Regulator PCB also provides +5VDC and +14VDC to the AC Regulator PCB.
Circuit Descriptions
This section contains a description of the circuitry for the following printed circuit boards:
Pre-Amplifier Module (PAM)
Signal Processor Module (SPM)
Cell Count Module (CCM)
Cable Distribution Module (CDM)
Solenoid Driver Module (SDM)
Motor Processor Module (MPM)
Stepper Drive Printed Circuit Board (SDP)
Pressure/Vacuum Regulator Module (PVRM)
Pump Relay Module (PRM)
Single Board Computer (SBC)
Data Link Adapter (DLA)
Pre-amplifier Module (PAM)

Note
Refer to PAM PCB Diagram.
PAM PCB Diagram
The PAM performs the following functions:
Provides constant current control to the von Behrens RBC/PLT and WBC Transducers.
Amplifies the initial RBC/PLT, WBC and HGB signals.
The constant current bias (+100VDC) is switched by U6, then routed to Q2 and Q3, which supplies constant current to the
von Behrens RBC/PLT transducers. The RBC/PLT current is adjusted with R72. Once received, the RBC/PLT signals are
initially amplified by U7, then routed to U5, where they are re-inverted and further amplified. The combined RBC/PLT signal is
then routed to the SPM PCB.
Transistors Q4, Q5 and associated circuitry provide constant current for the von Behrens WBC Transducer. The WBC
current is adjusted via R66. Once received, the WBC signal is initially amplified by U12, then routed to U11 where it is
re-inverted and further amplified. The WBC signal is then routed to the SPM PCB.
The output of the HGB Flow Cell is amplified by U1 and U2 prior to being routed to the SPM PCB. The HGB self test and gain
voltages are adjusted with R14 and R4 respectively.
Signal Processor Module (SPM)
Note
Refer to SPM Architecture.
SPM Architecture
EPLD (SPM)
The EPLD is an enhanced programmable logic device. The SPM EPLD is used to control data acquisition.
There are three state machines that run in the EPLD. One each is used for controlling data acquisition elements such as
Multiplexers and Peak/Hold Amplifiers. The inputs to the EPLD state machines are the outputs of the various Threshold and
Slope Detectors as well as Bubble and Area Comparators.
There is a data transfer state machine which controls data flow to the CCM.
Signal Processing
There are two signals from the RBC and WBC transducers that are processed through two basic data acquisition circuits.
The Platelet signal is derived from the RBC signal. There are three gain adjustments associated with each of these signals.
In addition to the three gain adjustments there is a integration adjustment for detecting pulses that are too long.
WBC Signal
The WBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the
differential amplifier but before the Test Signal injection point.
The WBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying
duty cycle of the blood cells.
A threshold detector signals to the EPLD the presence of valid pulses.
Simultaneously the Bubble detector signals if the pulse is determined to be a bubble. The bubbles are much larger
than pulses from blood cells. The EPLD discards the information from the pulse if it is determined to be a bubble.
The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector senses
another rising edge before the falling edge of the Threshold Detector. The data is processed immediately if there is
another rising edge before the falling edge of the Threshold Detector.
The WBC Held Peak is converted and sent to the CCM on a 15S cycle. Average pulses from the impedance
transducers are 35S.
RBC and PLT Signals

There are two modes for RBC/PLT data acquisition:
RBC MCV
RBC Count/PLT
RBC MCV
RBC MCV is for RBC MCV data only. In this mode, an integrator is enabled to determine if the cell is moving through the
middle of the aperture. If the cell is not moving through the center of the aperture, the data is falsely high and the pulse is
abnormally long. The integrated signal is compared to a proportion of its height. If the pulse is too long for its height, it is
discarded.
RBC Count/PLT
RBC Count mode is for count information. During this run, Platelets are counted simultaneous with RBCs and no integration
is used. The Platelet signal is derived from the RBC signal by an adjustable gain.
The RBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the
differential amplifier but before the Test Signal injection point.
The RBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying duty
cycle of the blood cells. At this point an additional gain stage is added to create the Platelet signal.
A threshold detector(s) signals to the EPLD the presence of valid pulses. The Platelet Threshold Detector is used if in
RBC Count mode.
If in Count mode, the Platelet Lower and Upper Threshold detectors are used. If the Upper Threshold Detector is
triggered, the pulse is a RBC and the RBC pulse is accumulated into the RBC Count histogram. If only the Lower
Detector is triggered, the PLT pulse is accumulated into the PLT histogram.
The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector(s)
senses another rising edge before the falling edge of the Threshold Detector. If this occurs, the data is processed
immediately.
The RBC Held Peak or the PLT Held Peak is converted and sent to the CCM on a 15s cycle.
Cell Count Module (CCM)

Note
Refer to PAM PCB Diagram.
CCM Architecture
System Clock and Microprocessor Description

The CCM uses a 16 MHz crystal as a time base. The fundamental cycle time for the MC68HC11KW1 processor is 4 MHz.
The processor has many built in functions such as:
16 bit address bus
8 bit data bus
7 - 8 bit multi-purpose I/O ports (CCM and SBC ports)
Small amounts of Internal RAM and Internal EEPROM (analyzer serial number)
Internal Timers (system timer)
Internal UART (debug port)
EPLD (CCM)
The EPLD is an enhanced programmable logic device and is used for address decode and histogram building functions. Its
program is used in coordination with the processor software.
LEDs
Note
Refer to CCM On-Board Diagnostic LEDs.
Scratch Pad RAM
This RAM is used for general purpose programming.
Histogram Memory
This RAM is used for histogram building.
Histogram Building (cell counting)
The following sequence is executed to build histograms:
1. Histogram Memory is cleared.
2. Counting is enabled.
This disconnects Histogram Memory from the processor buss.
3. Wait for an End of Conversion (EOC) signal from SPM.
4. At the EOC, data is read from the SPM ADC
5. The data then becomes the address for the histogram memory.
6. The data from the histogram memory is read.

This data is the count information for the respective pulse height.
7. The count is incremented and stored back into histogram memory.
8. Counting is disabled. And the histogram can be read by the processor.

HGB Measurement
A 12 bit ADC with an input multiplexer is used to measure the HGB signal from the PAM. This data is read by the processor.
Along with the HGB measurement, various DC voltages are read by the 12 bit ADC as a diagnostic.
CDM Port
The CDM port is connected directly to microprocessor ports E, G and K.
SBC Port
The SBC port is connected directly to microprocessor ports J and part of port A.
Cable Distribution Module
Note
Refer to Solenoid, Motor Drive and Pump Block Diagram.
Solenoid, Motor Drive and Pump Block Diagram
The CDM performs the following functions:
Status Sensor Interface
Control of Solenoid Driver Module
Pump Relay Module interface and control
Start Board (Touch Plate) Interface
The CDM communicates with the CCM via the CCM/CDM data bus at J2. Analog outputs of the Metering Modules are
converted to TTL levels by comparators (U12) and placed directly on the CCM/CDM data bus. Signals from the Pump Relay
board, Probe Position Switches, and Start Board (Touch Plate) are interfaced by Data Drivers (U5, U10).
Data is interfaced to the Solenoid Driver Modules via J32. This data is then multiplexed by One-of-Eight Decoders (U1, U2)
via J3, J4, J6, J7, and J9.
Vacuum and pressure control data is latched by U14 and routed to the Pump Relay Module via J11. Pump status signals
(Vac On, Pres On) are converted to TTL levels by U3 and placed on the data bus by U5.
LED drive signals are routed to the Start Board (Touch Plate) via J17. The start signal enters at J17 and is placed on the data
bus by U5.
Solenoid Driver Module (SDM)
Note
The purpose of the SDM is to provide drive current to the solenoids. Each SDM has eight Darlington drivers (Q1-Q8) which
are individually controlled by data bits (D0-D7) and data latch (U3).
There are two power modes available for each solenoid - activate (+28V) and hold (+14V). This is controlled by the Hi CLK
signal in conjunction with data bits (D0-D7) and the current control latch (U1).
Motor Processor Module (MPM)
Note
The MPM controls drive data to the Stepper Drive printed circuit boards and also provides self-test capability for motor
winding current. The MPM is comprised of the following major circuits:
Microprocessor
Program Control EPROM
I/O Peripheral Interface Adapter (PIA)
Direct Memory Access (DMA) Control
Motor Phase Latches
Motor Current Latches
Motor Winding Self Test
Control functions of the MPM are performed by microprocessor (U5).
The operating program for the microprocessor is stored in Program Control EPROM (U3).
Data communications between the CCM and MPM are controlled by I/O PIA (U6) and serial data is interfaced via ACIA (U2)
and Data Bus Connector (J1).
Phase data, motor direction, and step rate are stored in RAM (U7). This data is sent to the Motor Phase Latches under
control of the DMA Control circuitry, which consists of U11, U12, U15, U16, U18, U21 and associated circuitry. The data is
strobed into the appropriate Motor Phase Latch by ALG0 through ALG2.
The Motor Phase Latches U23, U26, and U29 provide phase data to the Stepper Drive printed circuit boards. Each is an 8-Bit
Addressable Latch which can control up to four Stepper Drive printed circuit boards and subsequently four Stepper Motors.
Four levels of motor current for each motor is controlled by the Motor Current Latches (U22, U25, and U28). Each latch can
control up to four stepper drive printed circuit boards. Data is strobed into the appropriate latch by WR0 through WR2.
The Feedback- and Feedback+ inputs at J3 through J14 are connected, via resistors on the Stepper Drive printed circuit
board, to the stepper motor windings. This allows the circuitry consisting of U30, U31, and U32 to monitor the winding current
during an internal self-test. These values can be read by the CCM to isolate a defective Stepper Drive or Stepper Motor.
Stepper Drive Printed Circuit Board
Note
Refer to MPM section, blocks 20, 23, and 24 of Solenoid, Motor Drive and Pump Block Diagram.
The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives a winding of the
Stepper Motor. Bits I0 and I1 are used to control four motor current levels:
P0 - High Current
P1 - Medium Current
P2 - Low Current
P3 - Current Off
Bits PH0 and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+ and Feedback- are used
to generate a motor self-test.
Pressure/Vacuum Regulator Module
Note
Pressure (or vacuum) is sensed by a transducer that is internally configured as a Wheatstone Bridge. Transistor Q1and
resistors R4 and R5 are used to generate a stable reference voltage for the Wheatstone Bridge. The output of the
Wheatstone Bridge is partially amplified (U1-7), stabilized against long term drift (voltage follower U1-1) and made
offset-adjustable by R18 and associated resistors.
Maximum transducer sensitivity can only be achieved when the output is zero volts at TP-1 and when there is no pressure
differential across the transducer. To accomplish this, R18 is adjusted for zero volts when both transducer inlet ports (P1 and
P2) are open to atmospheric pressure.
In order to maintain the operating point of comparator U2-14 at the fixed 2-volt trip level, it is necessary to maintain the output
of U2-8 within a relatively narrow range. This is accomplished by making the differential amplifier (whose inputs are U1-10
and U2-10) adjustable by selecting 1 of 4 possible jumper positions. A stable reference point for the DC operating level of
U1-8 and U2-8 is established by U1-14 in conjunction with R6 and resistor network RP1.
Measurement of pressure in the range of approximately 0.5 lbs/sq. inch is accomplished by using transducer inlet port P1 in
conjunction with jumper setting A/B.
Vacuum pressure in the range of 8 inch Hg is accomplished by using inlet port P2 in conjunction with jumper setting C/D. The
regulation point for either vacuum or pressure is established by the setting of potentiometer R16.
When the output of comparator U2-14 goes positive, the collector of Darlington transistor Q2 is pulled to ground, thereby
turning on either the pressure or vacuum pump. When the pumps are running, LED DS1 is lit and stays lit until either the
pressure or vacuum increases past the hysteresis point established by R8 of U2-14.
The output of the pressure/vacuum regulator can be inhibited by a logic low at J1-1. This completes the circuit description.
Note
Refer to major subsystem descriptions of AC and DC Power Distribution Subsystem.
Pump Relay Module (PRM)
The PRM provides drive to the vacuum and pressure pumps, via three Solid State Relays: K1, K2, and K3.
Single Board Computer (SBC)
The Single Board Computer (SBC) is designed around a Celeron 850MHz microprocessor and connects directly into the
Backplane PCB. The SBC receives power from the Power Supply Assembly via the Backplane PCB and receives status and
measurement data from the CCM through the DLA. The board contains EPROM, CMOS, RAM, input/output circuitry for the
interface ports, disk drives (hard and floppy) circuitry, LCD Display Screen Interface, and PS/2 Keyboard connectivity.
Data Link Adapter (DLA)
The Data Link Adapter provides interfaces from the SBC to both the CCM (Cell Count Module) and the Touch Pad
(membrane) keyboard. These two interfaces function independently under user interface software control. However, some of
the circuits on the DLA board are shared. The DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to the figure
below for an illustration of the DLA board.
DLA (Data Link Adapter) Block Diagram
Interface to CCM
A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel interface that is
software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (D0 - D7) in one direction at a time. The
SBC (Single Board Computer) initiates an inquiry message handshake on a periodic basis or when it has a command to send.
The CCM responds by sending data or replying to the command sent.
The interface is controlled cooperatively by the UIC and CCM according to the state of the handshake signals. Refer to Cell
Count Module (CCM). The UIC always sends data first. The CCM responds by sending data back (if any is available). Thus,
the 82C55 switches its A-port from input to output and back to input during every message transaction.
The key handshake signals are REQ1 and REQ2. REQ2 going low initiates the communication protocol. The CCM responds
by bringing REQ1 low. After the DLA has sent its data, it brings REQ2 high. Then the CCM sends its data.
Each byte received by the DLA generates an IRQ. The application software responds to the IRQ by putting the byte into a
buffer. When the CCM brings REQ1 high, the communication is complete; the DLA returns to an idle state, and port A is set
to input. Both REQ1 and REQ2 remains high until the next message/data transfer.
The two LEDs on the printed circuit board, DS1 and DS2, indicate the active state of REQ2 and REQ1, respectively. They
should always be flickering when the application software is running because the User Interface Software program is
constantly polling the CCM to check its state. (There are some exceptions to this during power up and certain diagnostics/test
modes.)
The I-O address of the DLA board is assigned by four jumpers. The default I-O address (pins 3 - 6 on S1 hard-wired) is: [off
off on off], 340 hexadecimal. The DLA interrupt level is assigned by a jumper.
Jumpers W1 - W6 assign the DLA interrupt to one of the following:
W#
IRQ #
W1
IRQ 5 *
W2
IRQ 10
W3
IRQ 10
W4
IRQ 11
W5
IRQ 12
W6
IRQ 15
* Since IRQ 5 is used by the DLA, W1 is hard-wired (this is the default).
Interface to Touch Pad (membrane) Keyboard

A 14-pin ribbon cable connects the DLA to the key panel. The key panel is also polled by the User Interface Software
program. This interface is not interrupt-controlled.
The keyboard is interfaced as a parallel switch matrix circuit with 4 lines out and 8 lines in. Four scan rows are driven active
one at a time via an LS175 latch. Then the columns of the key panel matrix are read from port B of the 82C55. The software
interprets a low active signal as a key is pressed.
The DLA board must be plugged into a 16-bit slot. There are no adjustments on the DLA board.
Troubleshooting
Diagnostics Menu Usage
Utilization of the DIAGNOSTICS Menu enables the operator and/or service representative to identify and correct both
operator-correctable and service-correctable faults. When the computer senses a fault, the message <NOT READY: SEE
DIAGNOSTICS> displays in the System Status Box. The following keys are available in the DIAGNOSTICS Menu.
Level One
INITIALIZATION: Used to perform an Initialization cycle: returns movable components to home position and
performs internal self-tests.
RAW DATA: Used to display raw measurement data for the last specimen.
COUNT TEST: Used to run specimens without returning to RUN Menu and display Raw Data.
MORE: Used to display additional functions (levels).
PRINTER OUTPUT: Used to toggle printer output ON and OFF.
HELP/ERROR: Used to display help information regarding the diagnostics menu screens. The fault log can also be
accessed through this key function.
MAIN: Used to return to Main Menu.
Level Two
WBC HISTOGRAM: Used to display WBC count and histogram data accumulated in each of 256 size channels.
RBC HISTOGRAM: Used to display RBC histogram data accumulated in each of 256 size channels.
PLT HISTOGRAM: Used to display PLT count and histogram data accumulated in each of 256 size channels.
SMOOTHING ON/OFF: Used to toggle histogram display status. With Smoothing Off, only raw counts are displayed.
With Smoothing On, channels are numbered, data is normalized and the number of the peak channel displays.
Smoothing On/Off (example) gives an example of Smoothing On/Off.
MORE: See Level One description.
PRINTER OUTPUT: See Level One description.
HELP/ERROR: See Level One description.
Level Three
PROBE HOME: Moves Sample Probe up and above RBC Cup. Displays probe assembly sensor status.
PROBE UP: Moves Sample Probe up. Displays probe assembly sensor status.
Level Four
SYSTEM STATUS: Used to display all pending alarms.
FAULT REPORT: Used to display all pending faults or warnings.
SERVICE HEX CODES: Hex system codes. Not used for operator or service troubleshooting.
SERVICE DEC CODE: Used to initiate individual actions in the CELL-DYN 1800 hardware and software.
Smoothing On/Off (example)
Fault Report Description

A detailed list of all faults generated by the CELL-DYN 1800 System software and hardware is contained in CELL-DYN 1800
Error Messages. The fault classifications reported in the Fault Report primarily contains data pertaining to the last CCM fault.
If a fault occurs, pressing the [HELP/ERROR] key immediately displays the [FAULT LOG] in the DIAGNOSTICS Menu. This
log may contain up to 16 faults, with the most current fault at the top of the list. An alternative procedure is to go to the MAIN
MENU and press [DIAGNOSTICS]. In this case, the [FAULT REPORT], not the Fault Log, is immediately displayed.
The Fault Log can be viewed from any of the menus, except SETUP. To view the Fault Log, enter the desired menu, followed
by [HELP/ERROR] and [FAULT LOG]. The system displays up to 16 past faults. From the MAIN MENU, press
[DIAGNOSTICS] followed by [MORE] three (3) times and [FAULT REPORT] to display the FAULT REPORT screen. A
display of <NO FAULTS OR WARNINGS PENDING> indicates that all faults have been cleared.
CELL-DYN 1800 Troubleshooting Guide
A list of symptoms, probable causes, and corrective actions for the most common problems encountered on the
CELL-DYN 1800 System is given in the Troubleshooting Chart. The probable causes and corrective actions for each
symptom are arranged in descending order from most likely to least likely. When troubleshooting a problem, start with the
most likely cause first.
If possible, thoroughly verify that a component is defective before replacement. Some problems can be verified visually, but
other problems may require a measurement tool such as a DVM (Digital Volt Meter).
When troubleshooting DATA PROBLEMS, only the measured parameters RBC, PLT, WBC, HGB, and MCV should be used
for reference. Using the calculated parameters can become confusing when trying to isolate a problem.
When troubleshooting CLOG AND FLOW ERROR PROBLEMS, refer to VP-04 Metering System Timing Adjustments - RBC
and WBC for the MIN and MAX specifications for the RBC and WBC Upper (T1) and Lower (T2) times.
Troubleshooting Charts
Nonfunctional Instrument Problems
Symptom
No Functions. No Fans
Probable Cause
Corrective Action
Power Cord
Check Power Cord
Power Source
Check Power Source
No 5VDC
Check that 5VDC LED on Card Cage Backplane is On

Note
If off replace Power Supply Assembly.
No Functions. Fans Run
Video Display Problems
Defective CCM
Replace CCM
Defective SBC
Replace SBC
Symptom
LCD Display Screen
Probable Cause
Corrective Action
Loose Connection
Check all LCD Display Screen Connections
Flash BIOS or
Connect a computer monitor to the top of the SBC PCB. Power ON
CMOS settings
instrument and open CMOS setup screen. Check that Advanced
reset to Default
Chipset Setup is configured correctly. Refer to VP-05 CMOS Setup
Blank/Solenoids OK
Verification/Adjustment.
Note
Do not measure voltage
on backlight inverter
Defective Backlight Replace Backlight Interconnect PCB

Interconnect PCB
PCB. Measuring voltage
Defective LCD
can damage PCB.
Interconnect PCB
Defective LCD
Replace LCD Interconnect PCB
Replace LCD Display Screen
Display Screen
Characters Out Of Focus
Defective LCD
Display Screen
Defective SBC
Replace SBC PCB
PCB
Characters OK But Garbled
Defective LCD
Display Screen
Defective SBC
Replace SBC PCB
PCB
Missing Characters
Defective LCD
Display Screen
Displayed Error and Fault Problems
Symptom
Probable Cause
Restriction
Corrective Action
Check Lines
Check In-Line Sensor
Detergent Empty
Defective In-Line Sensor
Replace Sensor PCB
Defective CDM
Replace CDM
Solenoid 3-1, 3-3, 3-4 Tubing
Check Solenoid 3-1, 3-3, 3-4
Diluent Empty
Restriction
Check Lines
Check In-Line Sensor
Defective In-Line Sensor

Defective CDM
Replace Sensor PCB

Replace CDM
Check Solenoid 3-3
Solenoid 3-3
Replace Solenoid 3-3

Check Solenoid 3-4
Solenoid 3-4

Check Solenoid 3-1
Pressure Overlimit
Solenoid 3-1

Check Pressure Switch
Defective Pressure Switch
Replace Switch
Defective CDM
Replace CDM
Leak 8" Hg
Check for air leaks in Fluid Power Supply and

Flow Panel
Vacuum Low Error
Defective Pump
Replace Pump
Defective Vac Regulator
Replace Vacuum Regulator
Leak 0.5 Psi
Check for air leaks in Fluid Power Supply and

Flow Panel
Pressure Low Error
Defective Pump
Replace Pump
Defective Pres Reg
Replace Pressure Regulator

Check Unreg Pressure
Waste Overflow Into

Accumulators
No Air Pressure
Replace Unreg Pump

Replace CDM
Check Solenoid 5-3
Solenoid 5-3

Check Solenoid 5-7
Solenoid 5-7

Check Solenoid 1-6
Solenoid 1-6
Check Accumulator Sensor Connections

Accumulator Sensor Falsely Detecting Flush Accumulator with DI Water
Liquid
Replace CDM
Check Internal Waste Sensor Connections
Defective Internal Waste Sensor
Replace Sensor
Check Unreg Pressure
Waste Empty Timeout
Unreg Air Pressure Low

Restriction
Replace Pump
Check tubing at Fluid Power Supply, Waste
Bottles, A and B
CCM/DLA Communication Error
Defective CDM
Replace CDM
DLA/CCM Cable connection
Check DLA/CCM Cable connection
Defective DLA/CCM Cable
Replace DLA/CCM Cable
Defective CCM Board
Replace CCM Board
Defective DLA Board
Replace DLA Board
Defective SBC PCB
Replace SBC PCB
Power Source
Check 5VDC and +12VDC (Digital) at Card Cage

Backplane PCB
Check CMOS Setup
Disk Errors (Hard Disk or Floppy

Drives)
Defective SBC PCB

Defective Disk Drive (Hard Disk or
Replace SBC PCB

Replace Disk Drive
Floppy Drives)
Defective Switch
Replace Switch and Perform Alignment
Defective CDM
Replace CDM
Run Motor Power test
Defective Stepper Drive Printed

Circuit Board
Replace Chopper Drive PCB
Position Faults
Exercise Probe (Diagnostic Menu, Probe Home)
Defective Sample Probe Assembly
Replace Sample Probe Assembly

Run Motor Power Test
Defective Motor
Replace Motor
Data Problems
Symptom
Probable Cause
Corrective Action
Check 12VDC (Analog) LEDs on Card Cage Backplane

PCB
All Results Are "0"
No 12VDC (Analog)
Replace Power Supply Module

Replace CCM
Check that PAM 100VDC LED is On
No 100VDC
HGB OK All Others "0"
Replace Power Supply Assembly
Defective SPM
Replace SPM
Defective PAM
Replace PAM
Reference lower than
Check for bubbles in Detergent line and Solenoid 2-6
sample reading
No sample aspiration
Check associated tubing, solenoids and sample syringe

Perform VP-08 Pre-Amplifier Module (PAM) Adjustment
HGB "0" all others OK
Defective PAM
Replace PAM
Defective CCM
Replace CCM
Defective HGB Flow Cell
Replace HGB Flow Cell

Check 0.5 psi (Bubble Mix) pressure
0.5 psi (Bubble Mix)
Check associated tubing and hardware
pressure
Perform Pressure Adjustment (0.5 psi)
Inadequate Probe Cleaning
Imprecision on all Parameters
Perform Sample Volume Verification

Imprecise Sample Aspiration
Imprecision on all Parameters,

HGB OK
Check associated wash block tubing and hardware
Check associated sample aspiration tubing and hardware
Imprecise Diluent Dispense
Perform Diluent Volume Verification
Carryover
Check for correct draining of Pre Mix Cup
Defective SPM
Replace SPM
Defective PAM
Replace PAM
Dirty RBC/PLT Transducer
Clean RBC/PLT Transducer
Imprecision on RBC and PLT,

WBC/HGB OK
Check 0.5 psi (Bubble Mix) Pressure

Incorrect 0.5 psi (Bubble
Mix) Pressure
Defective SPM
Replace SPM
Carryover
Check for correct draining of RBC/PLT Transducer
Dirty WBC Transducer
Clean WBC Transducer

Check 0.5 psi (Bubble Mix) Pressure
Incorrect 0.5 psi (Bubble

Imprecision on WBC/HGB,
Mix) Pressure
RBC and PLT OK

Defective SPM
Replace SPM
Carryover
Check for correct draining of WBC Transducer
Dirty Flow Cell
Clean Flow Cell

Check reference reading (Diagnostics Menu, Raw Data)
Reference reading out of

Imprecision on HGB, Others OK specification
Perform HGB Ref Adjustment on PAM VP-08 Pre-Amplifier

Module (PAM) Adjustment
Defective Flow Cell
Replace Flow Cell
Solenoid operation
Check Solenoid 3-6, 2-6 and 2-7
Dirty Aperture.
Clean RBC/PLT Transducer and Aperture Plate
RBC RER
Perform VP-09 Signal Processor Module (SPM)
Imprecision on MCV
Verification/Adjustment
Defective SPM
Replace SPM
Dirty Transducer
Clean WBC Transducer and Aperture Plate

Replace Lyse Syringe
Incorrect Lyse Volume

WBC "R" Codes, Reagents OK
WBC Gain
Replace Lyse Syringe Drive Assembly

Perform VP-09 Signal Processor Module (SPM)
Verification/Adjustment
Replace SPM
Defective PCBs
Replace CCM
Check power
High Electrical Backgrounds
Environmental
Check overhead lighting

Check for other items on same power line
Install filter (line conditioner)

Isolate line (dedicated line)
Check grounding cable on Front Cover
Poor instrument cover
Replace grounding cable
grounding
Check EMI Shielding on Card Cage Backplane PCB
Check correct routing of WBC and RBC/PLT Transducer
cables to PAM
Defective PAM
Replace PAM
Check Power Supply Module
"Noisy" Power Supply
Module
Replace Power Supply Module

Check Connections on Pre-Amplifier Filter PCB
Defective Pre-Amplifier Filter

PCB
Defective Transducer(s)
Replace Pre-Amplifier Filter PCB

Replace Transducer(s)
Clog and Flow Error Problems
Symptom
Probable Cause
Corrective Action
Perform VP-03 Vacuum and Pressure Adjustments

Incorrect vacuum
Diluent and Detergent lines
"CLOG" both sides constant
Check Solenoid 1-4 and 1-5 (closed during metering)

Check Reagent lines
reversed
Metering Tube position
Top of Metering Tube (tapered edge) should be visible on

top of upper DET sensor
Check Vent Tubing
"CLOG" T1 = Max T2 = 0, No Vent Restriction

Dirty Transducer
"CLOG" T1 = Max T2 = 0, No
Meniscus
Check Vent Solenoid 3-6, 2-1 and 1-3

Clean Transducer and Aperture Plate
Check plumbing
Restriction
Check Count Solenoid 1-2 and 4-3

Check Vent Solenoid 1-3 and 2-1
Check for bubbles on right side of Transducers

Dirty Transducer
Clean Transducer and Aperture Plate

Check associated tubing
"CLOG" T1 = Max T2 = 0, Slow
Restriction
Check Vent Solenoid 3-6
Meniscus
Perform VP-03 Vacuum and Pressure Adjustments
Incorrect Vacuum
"CLOG" T1 = Max Meniscus

speed OK
"CLOG" T1 =OK T2 = Max

Meniscus speed OK
"FLOW ERR" T1 = Min T2 = Max
"FLOW ERR" T1 = OK T2 = Min
Check Solenoid 1-4 and 1-5 (closed during metering)
Defective Upper Detector
Replace Metering Printed Circuit Board
Defective CDM
Replace CDM
Defective Lower Detector
Defective CDM
Replace CDM
Defective Upper Detector
Defective CDM
Replace CDM
Defective Lower Detector
Defective CDM
Replace CDM
Miscellaneous Problems
Symptom
Garbled Histograms
Probable Cause Corrective Action
Defective SPM
Replace SPM
Defective CCM
Replace CCM
Raw Data Description

From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key displays raw data obtained
from the last count cycle.
When a single count is done, all data is contained in the first column. When a PLT recount occurs, data from the first cycle
displays in column #2 and data from the recount displays in column #1.
Raw Data Display Description
RBC, WBC and PLT counts are RAW, uncorrected total counts.
HGB Error is not used.
HGB Reference is the output of the A/D Converter when reading reference (2000 = 5 volts).
HGB Sample is the output of the A/D Converter when reading sample (2000 = 5 volts).
WBC and RBC Up Times are the upper times in milliseconds for the last sample.
WBC and RBC Count Times are the times in milliseconds for the last sample.
Flow Error is coded Clog or Flow Error data.
RBC RER is RBC Cell Editing percentage.
WBC and RBC Upper max and Upper min are the maximum and minimum Upper Times, respectively.
WBC and RBC Avg. Time are the averages of the previous count times. The WBC and RBC Avg. time is reset when
the [Clear Orifice] key is pressed.
WBC and RBC Time-Outs are the floating Upper Clog Alarm Limits calculated by the "Running Average Program".
Note
An example of a raw data report displays in Raw Data (example).
Raw Data (example)
CCM On-Board Diagnostic LEDs

The seven LEDs on the CELL-DYN 1800 CCM can reveal much about the fundamental CCM and overall machine state. In
general, the LEDs indicate whether the CCM is in a normal functioning mode or in a fault state, and in either case, help to
characterize the CCM state. Also, LED2 gives some information about the state of the SBC.
The CCM tests itself on power-up. These fundamental tests include MC68HC11KW1, RAM, and SPM interfaces. If any test
fails, the CCM attempts to execute an endless loop routine which flashes the green LED on the board. Also, it places a 4-bit
fault code into the adjacent yellow LEDs (see Power-on LED Patterns - Fault States on Startup).
LED Function & Counting Control
The LEDs on the CCM (labeled LED1 through LED7) are entirely under program control. Their use is as follows:
Note
Refer to CELL-DYN 1800 CCM for location of LEDs on PCB.
LED7, a green LED, should not be not flashing after the CCM has successfully completed its internal power-on
self-check diagnostics, otherwise if it is flashing slowly (~1Hz.), there is a fundamental CCM fault.
LED5 and LED6, for CER and CEW, indicate the state of the CCM firmware generated signals CER (Count Enable
Red) and CEW (Count Enable White). These signals enable cell counting. When the associated LED is on, the DMA
cell counting circuitry is active.
LED3 and LED4, for WCP and RCP, are on during the metered count time intervals, LED3 for the white count time
interval and LED4 for the red count time interval.
LED2 is driven by the signal NREQ1, and indicates the UIC/CCM communication activity. This LED state directly
relates to the hi/lo state of NREQ1. When LED2 is flickering, which should always be the case during normal system
operation, it indicates that the UIC/CCM communications link is active. (There is a corresponding LED on the DLA
(UIC comm. board) that will also be flickering in sync with LED2; it is controlled by the DLA output signal NREQ2.)
LED1 is used to indicate that a self-test is in progress. This indicates that the tests for the pulse processing A to D
circuitry on the SPM with the pulse counting circuitry on the CCM are active.
Power-on LED Patterns - Normal Setup
LED2
LED3 LED4
LED5
LED6
LED7
Step
State
NREQ1
WCP
RCP
ON
ON
ON
CEW
CER
READY
ON
Power on
RAM testing
Flashing
Flashing
4
5
Flashing
Flashing
RBC/PLT testing
WBC testing
ON
ON
CCM tests done; Homing flowscript running
ON
ON
UIC program comm. started

Initialized (running/idle)
Power-on LED Patterns - Fault States on Startup
LED2
LED3 LED4 LED5 LED6
LED7
CCM
NREQ1 WCP
ON
RCP
CEW
CER
READY
Fault
ON
Flashing MC68HC11KW1 CONFIG reg
ON
Flashing MC68HC11KW1 RAM test
ON
Flashing 8K RAM bit test

Flashing 8K RAM clear test
ON
ON
ON
ON
ON
Flashing MC68HC11KW1 timer test

ON
CELL-DYN 1800 CCM
Flashing Histogram RAM test
Flashing CDM init. test
Cell Count Monitor (CCM) PCB
J6 - NOT USED
S1 RESET
LEDs 2-7
S3 BUFFALLO
10
E2 DGND
J2 MPM
11
J8 - NOT USED
J1 CDM
12
E3 DGND
J3 SPM
13
E1 DGND
J5 DLA
14
LED 1
CPU Hardware/Software Configuration

RS-232 Communications Test Procedure
Detailed information on the CELL-DYN 1800 System Interface Specifications is available on the WWCS Intranet website.
CMOS Setup
The CMOS Setup contains all the information needed by the Basic Input/Output System (BIOS) to establish proper
communications between the single board computer (SBC) and the various computer system devices. Refer to VP-05 CMOS
Setup Verification/Adjustment:
Special Function
Probe Check
There are two probe check functions activated by softkeys in the DIAGNOSTICS menu.
The [PROBE UP] softkey moves the probe up and maintains position without rotational motion. (The [PROBE
DOWN] softkey then displays to restore the probe to the down position.)
The [PROBE HOME] softkey places the probe over the RBC/PLT transducer. (The [PROBE DOWN] softkey then
displays to restore the probe to the down position.)
Note
Neither procedure puts the probe in the STANDBY position (on the left).
Service Special Commands

Discussion
Several commands are available to initiate individual actions in the CELL-DYN 1800 System hardware and software. These
commands are used for troubleshooting and/or alignment when a single action is desired or required to be repeated several
times.
The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press [DIAGNOSTICS] followed by
[MORE] three (3) times, then [SERVICE DEC CODE]. When this softkey is pressed, the message Test Select --- FOR
SERVICE USE ONLY --- displays.
A command can now be entered. Pressing the Enter key on the keyboard initiates the action. Only one command can be
entered at a time and [SERVICE DEC CODE] must be pressed before a command is entered.
All commands available by direct softkey can be accessed by pressing [MORE].
Note
Use only the commands listed in DEC Service Commands and always verify that the correct number has been
entered before initiating the action. Use only those numbers listed in DEC Service Commands. Other numbers may
refer to engineering commands which are not used in the field and which may cause damage if used improperly. Be
fully aware of the purpose of any of the DEC Service Commands before using them. This is a direct-activation
method which should be used with caution because the physical state of the CELL-DYN 1800 System may not be in
agreement with the function to be performed. After using service commands, always re-initialize the system by
turning the power OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to
ensure the instrument is in the proper configuration for normal operation.
DIAGNOSTICS Menu Service Code Function List

When the [SERVICE DEC CODE] key is pressed, the (Enter number (currently, 102):____ prompt displays.
The number above corresponds to the decimal code for the last code entered.
DEC Service Commands lists the decimal-coded (DEC) service commands that can be invoked by pressing the [SERVICE
DEC CODE] key in the DIAGNOSTICS Menu and entering the appropriate number.
DEC Service Commands
UIC DEC Codes
Function
07
NOT USED
08
NOT USED
09
NOT USED
11
NOT USED
15
fill lyse into system
16
NOT USED
17
NOT USED
18
NOT USED
19
fill Diluent & detergent
20
mini-wash
22
NOT USED
23
NOT USED
24
NOT USED
25
NOT USED
26
NOT USED
33
NOT USED
34
NOT USED
36
NOT USED
37
pre-dilute sample run setup
38
pre-dilute sample run exit
39
aperture current off (uses whole blood script)
40
open all valves
41
NOT USED
47
platelet recount
48
initialization (homing)
49
open sample run
50
clean orifice (back-flushing)
51
pre-dilute sample run
52
background count run
53
prime system with all reagents
54
daily shutdown
55
empty transducers and cups
56
gain adjust
57
unpinching normally closed valves
59
fill transducers and cups after empty
60
gain adjustment setup
61
dispense 10 ml saline
62
open sample wash
63
clean-for-shipping
64
clean sample syringe setup
65
aspirate 40 l sample for 1/250 dilution
66
dispense 10 ml saline for 1/250 dilution
67
aspirate 100 l sample for 1/50 dilution
68
dispense 5 ml for 1/50 dilution
69
NOT USED
71
lyse syringe down
72
NOT USED
73
NOT USED
74
lyse syringe up and home
75
lyse syringe down restore
76
pre-dilute sample wash
77
NOT USED
78
NOT USED
81
NOT USED
83
diluent syringe down
84
enzyme clean setup
85
probe up and rotate and home
86
back to ready position from probe home
87
probe up for probe adjustment
88
probe down (when finished, operator should initialize the instrument to place the probe in the home
position)
89
sample syringe up and restore
90
sample syringe down and home
91
enzyme clean the system
92
diluent syringe up and home
93
diluent syringe down and restore
117
NOT USED
118
NOT USED
119
NOT USED
120
NOT USED
121
cycle solenoids on waste assy
122
cycle solenoids on flow panel assy
123
sample syringe aspirate
124
sample syringe dispense
125
vacuum test
126
check mixing pressure
127
check backflush pump
128
motor power test (see Service DEC Code 128)
129
motor power level test (see Service DEC Code 129)
130
exercise motors (see Service DEC Code 130)
999
auto-cycle (see Auto-Cycling (Code 999))
Note
Certain commands are not sent to the CCM when the system is in an interlock state, such as STANDBY or
UNINITIALIZED.
Auto-Cycling (Code 999)

The CELL-DYN 1800 can be pre-set to do a specified number of RUN cycles without user intervention. This capability
applies only to normal RUN Count Test, Pre-Dilute RUN, (PRE-DIL TEST), Gain Adjust (GAIN ADJ), and Electrical
Background (ELEC BKGD). This capability helps reduce test time for the instrument. The following entry screen displays
after entering code 999:
-- Auto Cycle Test Set Up -Use Spacebar to accept current number
Use "<--" key to delete a digit
Use "ESC" key to cancel
Enter Number of Times to Repeat Test (currently, 10):
Sample Probe Description
The motors that enable the Sample Probe to move up/down and to rotate are stepper motors which are under direct
computer control. Since there is no direct positional feedback sent to the computer, position switches are employed to verify
critical positions during normal operation. It is important to understand that these switches only verify and do not control the
movement of the Sample Probe.
In the DIAGNOSTICS Menu, Service DEC Codes 128, 129, and 130 allow the service representative to control and exercise
all stepper motors in the CELL-DYN 1800 System. This description focuses on the Probe Up/Down Motor (B/2) and the
Probe Rotate Motor (C/3) which control the movement of the Sample Probe.
Service DEC Codes 128, 129, and 130 Descriptions
These commands reside in the SERVICE DEC CODE screen of the DIAGNOSTICS Menu and are used to test, control, and
exercise CELL-DYN 1800 stepper motors. A description of each of these three commands is given.
Service DEC Code 128
This code runs a computer generated test (Motor Power Test) of all stepper motors, motor driver boards, and associated
circuitry.
The Motor Power Test should be run whenever a problem is suspected with any assembly that is driven by a stepper motor.
The following entry screen displays after entering code 128:
Motor Power Test Started.
To MPM: {I }
To MPM: {pD32}
To MPM: {mC1!2} AC}
To MPM: {C1}
inp: 0415
A report (Motor Power Test (example)) automatically displays and can be printed. Refer to VP-11 Stepper Motor Power Test
and Verification.
Note
Press the [INITIALIZE] key before leaving the DIAGNOSTICS Menu.
Motor Power Test (example)
This code allows the Run and Idle power levels to be set when exercising a stepper motor. The four levels are:
0) Full Power
1) Medium Power
2) Low Power
3) Off
This code tests mechanical assemblies at various power levels or to remove idle power so the mechanism can be more
easily moved or checked manually. The Motor Power Level Test (example) screen displays after entering code 129 (press
the ENTER key after each entry):
Motor Power Level Test (example)
Note
After the entries are made, a message, such as Motor "A" set to running power of 1 and idle power of 3,
displays.
This code allows the direction, speed, and number of steps to be set when exercising a stepper motor.
The Motor Check (example) screen displays after entering code 130:
Motor Check (example)
Note
After the entries are made, a message, such as Motor "B": motion in direction "0" at speed "&" for 100 steps,
displays.
Motor Direction Commands
The table below contains information on the motor designation, command and direction of the motor to be tested. Motor
Speed Commands lists the motor speed commands to determine the speed of the motor being tested. Both tables are
needed to properly test the motor.
Motor Direction Commands
Motor Designations
A/1
B/2
C/3
D/4
Function
Sample Syringe
Probe Up/Down
Probe Rotation
Diluent Syringe
Command
Direction
Down/Aspirate
Up/Dispense
Up
Down
CCW/To RBC cup
CW/To Pre-Mixing Cup
Down/Aspirate
Up/Dispense
CCW/Dispense
E/5
Directional Valve
F/6
Spare
G/7
Spare
H/8
Lyse Syringe
Motor Speed Commands
Command Speed in Steps per Second
50
75
283
300
166
200
250
10
151
10
222
11
25
12
182
13
100
14
125
15
91
16
67
17
111
Sample Probe Normal Operation
CW/Aspirate
Down/Aspirate
Up/Dispense
Probe Up/Down INITILIZE and RUN Modes illustrates the Sample Probe's up/down sequence during the INITIALIZE and
RUN cycles. Probe Rotate "INITALIZE" Mode shows the probe's rotation movement during the INITIALIZE cycle.
Probe Up/Down INITILIZE and RUN Modes
Probe Rotate "INITALIZE" Mode
Initialization Mode
The Initialization cycle places mechanical and electrical components in the "home" position, drains any liquid in the tubing,
Pre-Mix Cup, and the Mixing Chamber of the von Behrens RBC Transducer to the Waste System, then places the instrument
in the INITIALIZED state.
Stepper Motor Homing
Homing a stepper motor is the process of setting up the initial position from which all future movement is referenced. In the
CELL-DYN 1800 System, this is accomplished by forcing the motor to move against a physical stop (Hard Stop). When the
mechanical assembly, driven by the motor, reaches the Hard Stop, the stepper motor electrically slips until it is forced to stop.
This mechanical position then becomes the zero reference position for the motor.
Operation:
1. The Sample Probe moves up at a fast speed until the Upper Switch (#2) is activated. It is then changed to a slow
speed, and homed against the Upper Hard Stop, which is the metal plate at the top of the Sample Probe Assembly.
2. The probe moves down six steps and the Upper Switch (#2) is checked.
3. The probe moves CCW at a fast speed until the Right Switch (#4) is activated. It is then changed to a slow speed,
and homed against the Right Hard Stop, which is the mounting bracket for Right Switch (#4).
4. The probe moves CW to the Pre-Mix Cup and Left Switch (#3) is checked. The probe then moves into the Pre-Mixing
Cup.
5. The probe moves up and Upper Switch (#2) is checked.
6. The probe moves CCW to center and down positions; and the Lower Switch (#1) is checked.
7. This completes the Initialization cycle.

Run Mode
The figure below illustrates the probe's movements during the RUN cycle.
Probe Rotate "RUN" Mode
Operation:
1. When the Start Switch is pressed, 30 L of sample is aspirated and Lower Switch (#1) is checked.
2. The Sample Probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
3. The probe moves CW to Pre-Mix Cup and Left Switch (#3) is checked.
4. The probe moves down eight steps and into the Pre-Mix Cup, where dispense, probe shake, and aspiration of RBC
sample takes place.
5. The probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
6. The probe moves CCW to the Mixing Chamber of the von Behrens RBC/PLT Transducer, stops three steps from
Right Hard Stop, and Right Switch (#4) is checked.
7. The probe moves down into the RBC/PLT Mixing Chamber and RBC sample is dispensed.
8. The probe moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2) is checked.
9. After completion of the count cycle, the probe moves CW to center position.
10. The probe moves down and Lower Switch (#1) is checked.
11.This completes the RUN cycle.

Switch Failure Descriptions
Example of fault reports are shown in the following figures:
Lower Switch (#1) Fault Report

Upper Switch (#2) Fault Report
Left Switch (#3) Fault Report
Right Switch (#4) Fault Report
When a switch is checked by the computer and found to be deactivated (open) in normal operation, the message "Not Ready:
SEE DIAGNOSTICS" displays on the RUN Menu.
From the MAIN MENU, press [DIAGNOSTICS]. The screen immediately displays one of the Fault Reports shown in Lower
Switch (#1) Fault Report, Upper Switch (#2) Fault Report, Left Switch (#3) Fault Report, and Right Switch (#4) Fault Report.
The message <SWITCH: 1 CHECK> indicates that Lower Switch (#1) failed when checked. The message <* NOT ON ANY
SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to Lower Switch (#1) Fault
Report.
Lower Switch (#1) Fault Report
The message <SWITCH: 2 CHECK> indicates that Upper Switch (#2) failed when checked. The message <* NOT ON ANY
SWITCH *> indicates that none of the switches were activated when the failure occurred. Refer to Upper Switch (#2) Fault
Report.
Upper Switch (#2) Fault Report

The message <SWITCH: 3 CHECK> indicates that Left Switch (#3) failed when checked. The message <ON SWITCH(ES):
2 TOP> indicates that Left Switch (#3) was activated when the failure occurred. Refer to Left Switch (#3) Fault Report.
Left Switch (#3) Fault Report

The message <SWITCH: 4 CHECK> indicates that Right Switch (#4) failed when checked. The message <ON SWITCH(ES):
2 TOP> indicates Right Switch (#4) was activated when the failure occurred. Refer to Right Switch (#4) Fault Report.
Right Switch (#4) Fault Report

The above conditions do not necessarily indicate that a switch has actually failed. They only indicate that the switch was not
read as activated when checked by the computer. A failure could also be caused by improper switch alignment, an electronic
hardware failure, or a mechanical hardware failure.
CELL-DYN 1800 Error Messages

The table below lists error messages on the CELL-DYN 1800 System.
Description
Error Message
(Status box)
Time-out at N seconds
A CCM process initiated by the user took longer to complete than allowed (usually indicating
a failure of the CCM). The process ran approximately N seconds before the time-out
occurred.
Process Aborted
A count test was stopped either by the user or because of a fault detected by the CCM.
Fix then press [CLEAR
A user-correctable fault condition was detected.
ALARM]
Process Monitoring Aborted
A process was stopped by the user (using the asterisk (*) key.
Error Message
Description of the error.
(Display Area II)

Printer Time-out
The Printer Output option was ON and the printer did not print the requested report in the
expected time.
Code N is Invalid
The user has entered a command for the CCM whose numeric value exceeds 127. The
value entered was N.
Cannot do this Function
The user has attempted to issue a command to the CCM that cannot be executed because
of a pending fault condition.
WBC Meniscus Detection,
During the most recent count, a meniscus was not detected or was detected at an
RBC Meniscus Detection
unexpected time.
WBC Count Time-out (clog),
During the most recent count, a Clog occurred.
RBC Count Time-out (clog)

CCM Pulse Height Memory
During the most recent count, there was an overflow in one of the pulse-height arrays
Saturation Warning
(histograms).
External Waste Full
The external waste bottle has been filled.
Lyse Empty, Detergent
The indicated reagent has run out.
Empty, Diluent Empty

Invalid Alarm Set
A bit was set in the fault message from the CCM that has no valid interpretation.
*NOT ON ANY SWITCH*
After some mechanical motion, a reading of all the position sensors indicates that none are
activated. (This message does not necessarily mean that a mechanical fault has occurred.)
Waste Overflow Into
A reading of the sensor in the Waste Accumulator suggests that there is liquid in the
Accumulators
accumulator.
Error Message (Status box)
Description
Vacuum
There was a vacuum failure during power-up or the instrument is unable to maintain vacuum
level while in the READY state.
Pressure
There was a pressure failure during power-up.
Position Fault
A mechanical assembly is not in the correct position for the most recent function to be
performed, as indicated by position sensors.
Sensor Fault - Internal Waste
A Time-Out fault occurred in draining one of the waste bottles. This error is also associated
Empty
with positive pressure.
Canceling Auto-Cycling
This message displays when the user cancels Auto-Count Testing.
Invalid UIC Command Sent to The UIC sent a command to the CCM that it cannot interpret.
CCM
Error in Flow System Timing
An error in the timing of a flow script has occurred. This occurs during instrument
initialization when the flow script takes more than three minutes to complete.
Histogram Memory Clear
The CCM was unable to clear the pulse-height memory.
CCM Program, RAM Memory
The CCM detected a failure in its RAM.
CCM/MPM Message Fault

Other error-related messages
MPM to CCM, Message
Transmit Error
Command to be Sent to MPM
is Incorrect
An error in CCM/MPM interprocessor communications occurred. A fault was generated in an
Abnormal Time-out/No MPM
attempt to send or receive motor or other command to or from MPM, or the MPM was unable
Response
to perform the function.
Fault Response from MPM

Incorrect Command to be
Sent to MPM
Attempt to Send MPM a New
Command while Busy
No Such Script in ROM or
Error in loading a flow script.
RAM
CCM/UIC Message Fault
An error in UIC/CCM interprocessor communications occurred.
No Response from CCM
The CCM is not functioning or the signal cable connecting the CCM and UIC is faulty or
disconnected. Turn the instrument OFF, check the CCM/UIC cable, then turn the instrument
ON.
CCM is Initializing
The CCM is in the middle of its Initialization process.
Undefined Event
An undefined event or process occurred.
Count Test
The [COUNT TEST] key in the DIAGNOSTICS Menu is used to run specimens and display Count Check data without
returning to the RUN Menu. Coded data relating to specific cycle functions, raw measurement, and flow count time are
displayed for use in troubleshooting or service.
Event Messages During Diagnostic Menu Count Test lists the event messages that are displayed during the Diagnostic Menu
Count Test.
Event Messages During Diagnostic Menu Count Test
Event Messages
Description
SampSw. pressed
Touch Plate was pressed.
Remove specimen
Specimen should be removed.
Count valve open
The counting valve is open.
RBC histogram avail RBC histogram is available.

WBC upper det
WBC upper meniscus detection.
WBC lower det
WBC lower meniscus detection.
RBC upper det
RBC upper meniscus detection.
RBC lower det
RBC lower meniscus detection.
Plt recount strt
Platelet recount starts.
CCM initing
CCM initializing.
Data invalid
Data entered is invalid.
WBC histo avail
WBC histogram is available.
Proc complete
Process is completed.
Data avail
Data is available.
CCM init done
CCM initialization is completed.
Canceled
Canceled operation.
Operator-Correctable Alarm or Fault Messages

This table lists operator-correctable alarm or fault messages.
Event Messages
Description
External Waste Full Waste full sensor is activated.

Detergent Low
Detergent is low as detected by reagent sensor in reagent inlet tube.
Diluent Low
Diluent is low as detected by reagent sensor in reagent inlet tube.
Lyse Empty
No lyse is detected by reagent sensor in reagent inlet tube.
Invalid alarm set
Incorrect error or message was set.
Detergent Empty
No detergent is detected by reagent sensor in reagent inlet tube.
Diluent Empty
No diluent is detected by reagent sensor in reagent inlet tube.
CCM is initializing
CCM module is currently in the INITIALIZED state.
Summary of Error Messages

Summary of Error Messages gives a complete listing of error messages available on the CELL-DYN 1800 System.
Summary of Error Messages
Error Message (Status box)
DOS Errors
Arithmetic overflow
Bad drive request structure length
Cannot remove current directory
Cannot rename across drives
Collection index out of range
Collection overflow
CRC error in data
Device read fault
Device write fault
Disk full
Disk is write-protected
Disk read
Disk seek
Division by zero
Drive not ready
File access denied
File not assigned
File not found
File not open
File not open for input

File not open for output
Floating point overflow
Floating point underflow
Hardware failure
Heap overflow
Invalid drive number
Invalid file access code
Invalid file handle
Invalid floating point operation
Invalid function number
Invalid numeric format
Invalid pointer operation
Path not found
Printer out of paper
Range check
Sector not found
Stack overflow
Too many open files
Range check
Sector not found
Stack overflow
Too many open files
Unknown command
Unknown media type
Unknown unit
General
Demographic entry disabled
No data to print
Stopping
Unable to Load Flowscript
Unable to Save Cal Factors
Unable to Set Volume
System Fault
Not Ready: See DIAGNOSTICS
Uninitialized
Normal Operator-Correctable
Detergent empty
Diluent empty
Lyse empty
Waste full
DLA Faults
<-? Invalid error code
CCM command echoed does not match
CCM REQ1 high during data send at char
CCM REQ1 is stuck LOW
DLA buffer overflow on receiving char
DLA/CCM error on command
Incorrect checksum on CCM data receive
NAK received from CCM
No response from CCM
Time-out 1 on wait for CCM REQ1
Time-out 2 on message send to CCM
Time-out 3 on message receive from CCM
Date
Battery failure or system date/time not set
Disk I/O
Cannot open CD1800.ini configuration file
Cannot read CD1800.ini configuration file
Cannot write to CD1800.ini configuration file
CD1800.ini configuration file size error
CD1800.ini configuration file version error
Configuration file error
Error when reading CRC values from disk
Memory error when creating CRC tables
Ticket Printer
Data err
Inc. asp.
No Ticket Detected
QC Log
Can not accept specimen
Can not reject specimen
No loading, QC file has to be empty
Purge log failed
Read QC file failed
Data Log
Cannot do if uninitialized
Cannot write Data Log header to disk
Count overrange
Data Log write error
Failed to read from Data Log
Flow err
Initialize
Data Log initialization failed
QC Log initialization failed
Communications
Break interrupt
Comm open error
Comm error
Framing error
Parity error
Re-transmit started
Re-transmit time-out
Receiver overrun
Transmit started
Unable to re-transmit
Printer Driver
Printer Fault
Printer Not Ready
Printer Off-line
Printer Out Of Paper
Printer Time-out
Ticket Printer Not Ready
QC Log I/O
Failure to read from QC Log
Failure to write to QC Log
QC Log size error
QC Log version error, bytes short for creation of QC Log
Help
Unable to open help file
Reagent Log
Unable to create file
Unable to open file
Data Log Errors

Cannot Access Data Log
CRC Reading Failed
Data Log initialize failed, bytes short for creation of Data Log
Data Log Print Error
Data Log size error, Call Technical Support
Data Log version error
Data Transmission Error
Not enough memory error
X-B File
Failed to write to Data Log Header
Failed to write to Data Log
No response from CCM
Data Log I/O

Data Log write failure
Write Header failure
Write version error
General Faults
Abnormal time-out / no MPM response

Attempt to send MPM a new command while busy
CCM pulse height memory saturation warning
CCM is in Fault State
CCM is in Unknown State
CCM Program, RAM Memory Fault
CCM real time clock has failed
CCM/DLA Message Communication Fault
Command to be sent to MPM is incorrect
Count Overrange
Count time-out (clog)
Data Capture Fault
Detected Probe Assembly Switch: # in Incorrect State
Detergent sensor
Diluent sensor
Error in Flow System Timing
Fault response from MPM
Guard electrode voltage warning
Histogram Memory Clear Failure
Incomplete Aspiration
Incorrect command to be sent to MPM
Initial Communication with DLA Failed
Invalid Command Sent to CCM
Invalid Data
Mechanical Position Fault
MPM to CCM, message transmit error
No response from MPM
Press [INITIALIZATION] key to clear fault and re-initialize meniscus detector: "True" sensed at metering start meniscus: not
detected during valid time interval
Pressure Level Time-out
Pressure Over-Limit Detected

Printer is not Ready: Cannot RUN uninitialized
RBC Clog
RBC Flow Error
Run time error: # at:
Time out after approximately __ minutes
Turn off instrument and drain accumulators manually
Unexpected response from MPM
Unknown MPM/CCM fault
Vacuum Level Time-out
Waste Drain to Empty Time-out
Waste Overflow into accumulators
WBC Clog
WBC Flow Error
Wrong software for this instrument
Fault Log
Error in writing to Fault Log file
Error in writing header to Fault Log file
Fault Log I/O

Allocating heap memory error
Bytes short to create the Fault Log
Fault Log file size error
Fault Log Header IO Error
Incorrect version of Fault Log
Lab ID Setup
Drive A is not ready
Fail to read assay file
Fail to write LAB ID file

Incorrect assay format
Incorrect disk. QC values do not apply to this instrument
Incorrect Exp. Date
Incorrect Lot Number
Incorrect parameter in assay file
Software Commands and Sequence

CD1800 File and Directory Structure
Accessing DOS
There are two ways to access the DOS program from the CD1800 program:
With the CD1800 program still loaded,
By exiting the CD1800 to DOS. The Exiting from CD1800 to DOS method is the preferred method.
Switching from CD1800 to DOS

1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.
2. Press ESC on the PC keyboard. The following message displays on the screen.
CD1800 Program Exit

It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the
instrument off after doing any service work while in DOS.
3. Press the Alt and d keys simultaneously. The DOS prompt C:\CD1800> displays.
4. Execute the desired DOS commands. Most commands can be executed. However, computer memory is limited
since the CD1800 program is still loaded.
Exiting from CD1800 to DOS
2. Press ESC on the PC keyboard. The following message displays on the screen.
CD1800 Program Exit
It is recommended that the Cell-Dyn be put in Stand-by before exiting the program. Otherwise, turn the
instrument off after doing any service work while in DOS.
3. Press the Alt and x keys simultaneously. The CD1800 program is removed from computer memory. The DOS
prompt (C:\1800>) displays.
4. Execute the desired DOS commands.

Exiting DOS
To exit the DOS program and return to the CD1800 program in the case where the CD1800 program remains loaded
(Alt and d was used to access DOS), type exit at the C:\> prompt and press the Enter key. The DIAGNOSTICS Menu
displays.
To exit the DOS program in the case where the CD1800 program is not loaded, (Alt and x was used to exit the CD1800
program), turn the instrument OFF then ON again to reinitialize the system.
Note
Turning the instrument OFF then ON again prepares the system for normal operation.
Common DOS Commands
Function
Change drives
Change directory
Copy
Command
c: or a:
cd
copy
Description
switch C and A drives

filename
source to destination
Delete
del
file name(s)
Directory
dir
current or specified drive
Exit
exit
quits the current program
Undelete
undelete
file name
DOS Command Usage

Change Drives
When accessing DOS from the CD1800 program, the C:\> prompt displays, indicating the C drive is selected. To change to
the A drive (floppy disk), type A: and press Enter. The prompt changes to A:\> indicating the A drive is selected.
Change Directories/Files Within a Drive
To move from a directory to the root directory (C:\) type cd\ and press the Enter key.
To move one level closer to the root directory type cd.. (where .. are two periods) and press the Enter key. For example, to
move from CD1800\HELP to CD1800 subdirectory type cd.. and press the Enter key.
To move one level away from the root directory type cd_[directory name] (where _ indicates a space) and press the Enter
key. For example, to move from the root directory (c:\) to the CD1800 directory type cd cd1800 and press the Enter key.
Copy
To copy a file on the CELL-DYN 1800 hard drive (C drive) to a floppy disk (on the A drive):
1. Exit the CD1800 program procedure above. Change to A drive, if the file to be copied is on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be copied.
3. At the C:\> prompt, type Copy_C:\[filename]_A:\ (where [filename] is the complete file name and _ indicates a
space between characters).
Example: to copy file CONFIG.SYS from the C drive to the A drive, type copy c:\config.sys a:\ and press the Enter
key.
To copy this file from the A drive to the C drive, type copy a:\config.sys c:\ and press the Enter key.
Delete a File
To delete a file:
1. Access DOS (Exiting from CD1800 to DOS). Change to A drive if the file to be deleted is on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be deleted.
3. At the C:\> prompt, type delete_ [filename] (where [filename] is the complete file name and _ indicates a space
between characters) and press the Enter key. For example, to delete a file called Sample from the C drive type
delete sample and press the Enter key.
Undelete a File
To undelete a file which had previously been deleted:
1. At the C:\> prompt, type undelete_ [filename] (where [filename] is the complete file name and _ indicates a space
between characters) and press the Enter key. For example, to undelete a file called Sample from the C drive, type
undelete sample and press the Enter key.
View Files in a Directory
To view all the files in a particular directory:
1. Access DOS (Exiting from CD1800 to DOS). Change to A drive if the files to be viewed are on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be viewed.
3. At the C:\> prompt type dir and press the Enter key.
Note
If there are many files, the file names scroll down the screen too rapidly for the user to see. The user can prevent this
by adding "switches" to the DIR command using the format: DIR_[switch] (where the underscore ("_") indicates a
space). For example, to display the file names a screen at a time, type dir_/p and press the Enter key. To display the
file names in columns across the width of the page: type dir_/w and press the Enter key.
Engineering Drawings and Schematics

CD1800
CELL-DYN 1800 PCB Reference

AC Regulator PCB
AC Regulator PCB
14
J1
J2
15
J3
TP6
16
J8
TP7 (+14V)
17
LD3 (+14V)
TP5 (VR)
18
LD4 (+5V)
TP4 (GND)
19
J5
R22
20
LD1 (+12V)
J4
21
LD2 (-12V)
J7
22
TP2 (GND)
10
TP9 (GND)
23
TP3 (+12V)
11
TP8 (+100V)
24
TP1 (-12V)
12
R28
25
J6
13
J9
Backlight PCB
Backlight PCB
CN1
CN2
Backplane PCB (front)
Backplane PCB
LD4 (+12V Digital)
J2
LD5 (-12V Digital)
SBC
LD2 (+12V Analog)
DLA
10
LD3 (-12V Analog)
CCM
SPM
11
LD1 (+5V)
Backplane PCB (rear)
Backplane PCB
TP3 (-12V Analog)
J1
TP2 (+12V Analog)
TP6 (DGND)
J10
TP1 (+5V)
10
J9
TP5 (-12V Digital)
11
J11
TP4 (+12V Digital)
CCM PCB
CCM PCB
J6
S1 (RESET)
LEDs 2-7
S3 (BUFALLO)
10
E2 (DGND)
J2 (MPM)
11
J8
J1 (CDM)
12
E3 (DGND)
J3 (SPM)
13
E1 (DGND)
J5 (DLA)
14
LED1
CDM PCB
CDM PCB
18
J25
TP1 (GND)
19
J26
J11
20
J27
J12
21
J28
J13
22
J29
J14
23
J30
J15
24
J31
J16
25
J10
J17
26
J9
10
J18
27
J8
11
J19
28
J7
12
J32
29
J6
13
J20
30
J5
14
J21
31
J4
15
J22
32
J3
16
J23
33
J2
17
J24
34
J1
DC Regulator PCB
DC Regulator PCB
J1
LD2 (+14V)
10
J11
J5
11
LD3 (+12V)
J7
12
J8
J6
13
J9
J4
14
J10
J2
15
LD1 (+5V)
J3
DLA PCB
DLA PCB
DS2 LED
J1
J2
TP1
TP2
DS1 LED
Indicator PCB
Indicator PCB
LD2 (yellow)
LD1 (green)
LD3 (red)
Internal Waste Empty PCB
Internal Waste Empty PCB
J1
E2
J2
E1
LCD Interconnect PCB
1 LCD Interconnect PCB
J2
2 J8
J3
3 J7
JP1B
4 J5
JP1A
5 J6
10 J4 (USED FOR CELL-DYN 1800)
MPM PCB
MPM PCB
TP4 (+28V)
DS1 thru DS4 LED'S
TP2 (GND)
TP1 (GND)
TP6 (+12V)
TP3 (RESET)
10
J2
J3 thru J14
11
J1
TP7 (+5V)
PAM PCB
PAM PCB
13
TP3 (GND)
J3
14
JMP3
J4
15
R14
JMP1
16
J5
J1
17
R72
TP7
18
LD3
R4
19
TP5 (GND)
TP1
20
R66
JMP2
21
TP6 (GND)
10
TP2
22
LD1 (-12V)
11
J2
23
LD2 (+12V)
12
TP8 (GND)
24
TP4 (+100V)
Pre-Amp Filter PCB
Pre-Amp Filter PCB
-12V
+12V
GND
PRM PCB
PRM PCB
J1
J5
J2
J8
J3
J7
J4
J6
10
F1
RBC Metering PCB
J1
RBC Metering PCB
TP2
J1
TP3
TP1
Reagent Empty Sensor PCB
Reagent Empty Sensor PCB
J1
E1
E6
E2
E5
E3
E4
SBC PCB
SBC PCB
LPT1
Fan
COM1
Power
COM2
Hard Disk Drive
PC Keyboard
Floppy Disk Drive
10
LCD Display Screen
SDM PCB
SDM PCB
DS1 thru DS8 LEDs
TP2 (DGND)
J1 thru J8
J9
J10
TP1 PWR GND
SPM PCB
SPM PCB
E2 (AGND)
R22 (WBC)
J1
R27 (RBC)
J4
R41 (PLT)
E1 (DGND)
R51 (RER)
10
E3 (AGND)
Touchpad PCB
Touchpad PCB
J1
J2
Vacuum/Pressure Regulator PCB (front)
Vacuum /Pressure Regulator PCB
J1
P2
DS1
P1
R18
TP3
TP2
TP1
10
E1
Vacuum/Pressure Regulator PCB (rear)
Vacuum /Pressure Regulator PCB
WBC Metering PCB
R16
WBC Metering PCB
J1
TP1
TP3 (GND)
TP2
Removal & Replacement

Service Equipment Required
Every system subassembly and module can be removed and replaced using the tools listed below. Substitute equipment
having the same characteristics as those listed can also be used. Whenever possible, choose non-magnetic tools to avoid
damaging sensitive printed circuit board mounted components and magnetic media.
Required Tool Recommended Model
Description
Cable Ties
Self-locking
Flashlight
Pen light
Knife
Utility
Nut driver
1/4"
8-inch
Nut driver
1/2"
8-inch
Pliers
5"
Diagonal Cut
Pliers
8"
Long Nose
Screwdriver
Phillips No. 2
18-inch
Screwdriver
Phillips No. 1
Stubby
Screwdriver
Phillips No. 2
Stubby
Screwdriver
Slotted 3/16 X 10
8-inch
Screwdriver
Slotted 3/16 X 8
8-inch
Screwdriver
Hex 7/64"
8-inch
Spare tubing
Silicone
Wrench
Various lengths
Adjustable
Covers (CD18-A1)
Removal
Action
Steps
Reference
1. Grasp under the lower right side [2] and
Remove
Upper Front
open the cover [1] toward the left.
Cover
Note
Cover is hinged on the left side and is
kept closed with a magnetic latch.
2. Disconnect cable connector J1 [3] on
the Status PCB [4].
3. Disconnect the ground wire [5] from the
instrument chassis.
4. Use a slotted screwdriver to remove the

C clip [6] from the lower hinge.
Note
Be careful when removing the C clip.
The hinge contains a plastic washer [7]
and a spring [8] which can be lost.
5. Remove the plastic washer and spring.
6. Lift the cover from the hinge pegs and

remove it from the instrument.
Replacement
Action
Install Upper Front Cover
Steps
1. Install in reverse order of disassembly.
Reference
Verification
Action
Steps
Reference
Verification of Proper Cover
1. Place instrument into READY mode.
Installation
2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then
NORMAL BACKGRND.
3. Press the touch plate to run a cycle.

Note
During the cycle, make sure that cover does not interfere with
instrument operation.
4. Once cycle is complete, verify acceptable background results. Refer to

Specifications & Checklist.
Removal
Action
Open Upper
Steps
1. Open the upper front cover.
Front Cover
Check
Caution
Sample
Be sure the sample probe is out of the
Probe
way before attempting to remove the
Position
lower front cover to prevent damage to

the probe.
If the instrument is ON, from the MAIN

MENU press [SPECIAL PROTOCOLS]
then [MORE] followed by [PROBE
HOME] to place the probe over the
RBC/PLT Mixing Chamber.
If the instrument is OFF, make sure the

Sample Probe is raised all the way (if not,
turn the lead screw to raise the probe) so
Reference
that it clears the lower front panel.
Remove
1. Remove the thumbscrew [2] from the
Lower Front
lower front cover (to the bezel).
2. Slide the lower front cover [1] to the left
Cover
approximately 1/2-inch until it clears the

bezel, then lift up and out.
Note
It may be necessary to lift the right side of
the cover to clear the bezel indentation
while sliding the cover to the left.
3. Verify that the upper left hand corner of

the cover clears the clip. [3]
Replacement
Action
Install Lower Front Cover
Steps
Reference
Verification
Action
Steps
1. Place instrument into READY mode.
Installation
2. From the MAIN Menu, press RUN followed by SPECIMEN TYPE, then
NORMAL BACKGRND.

Note
During the cycle, make sure that cover does not interfere with
instrument operation.
4. Once cycle is complete, verify acceptable background results. Refer to

Specifications & Checklist.
Reference
Removal
Action
Remove
Top Cover
Steps
Reference
1. Using a Phillips-head screwdriver, remove

the two (2) mounting screws [2] on the rear
of the instrument securing the top cover to
the chassis.
2. Lift and remove the top cover [1] by sliding

it to the rear.
Replacement
Action
Install Top Cover
Steps
Reference
Verification
Action
Steps
Verification of
1. Verify that cutouts on the front
Proper Cover
portion of the top cover [1] are
Installation
placed under the locking nuts [2].
2. Be sure that rear of the cover is

flush against the rear instrument
panel.
Reference
Removal
Action
Steps
Open Upper
1. Open the upper front cover to the left.
Front Cover
Remove
Lower Front
1. Remove Lower Front Cover (RR-A1.02

Lower Front Cover).
Cover
Remove
Right Bezel
the left screw [2] on the right side
Screw
cover [3].
Note
The Phillips screw secures the bezel to
the instrument.
Remove
1. Lift the bezel up and away from the
Reference
Bezel
instrument.
Note
Do not pull bezel too far away from
instrument. Several cables must be
removed before completely removing the
bezel from the instrument.
2. Disconnect the following:

o
Ground wire to chassis [7]
Power [3] and data [2] (ribbon)

cable connectors from the rear of
the floppy disk drive [4]
Ribbon cable connector J2 [5] on

the Touch Pad Interconnect
PCB [6].
Note
Mark all cable connectors and note pin 1

(red stripe on ribbon) on the floppy drive
data cable.
3. Remove the screw [8] (Phillips-head)
securing the LCD Interconnect PCB
access cover [1].
4. Disconnect the ribbon cable connected to
JP1A [9] on the LCD Interconnect
PCB [10].
5. Remove the bezel.
Replacement
Action
Install
Steps
Bezel
Note
During assembly, verify that all cables/connections are secured and are not pinched
between the cover and the chassis.
Verification
Reference
Action
Steps
Reference
Verification of Membrane
1. Power ON instrument, and allow it to initialize.
Keypad Operation
2. During initialization, verify that the LED on the floppy disk drive turns on
and that the LCD display screen is operational.
3. Once instrument is initialized, press RUN to prime system.
4. When in READY mode, press keys on membrane to check for proper
instrument response.
5. If system does not respond, check membrane keypad connection.
Removal
Action
Remove
Steps
Reference
1. Remove all cable connectors
Right Cover
(keyboard [3], COM1 [4], COM2 [5],

LPT1 [6]) from the right side of the
instrument.
the left and right screws [2] securing the
right side cover to the instrument frame.
3. Slide the right side cover [1] to the rear of

the instrument about 1/2-inch to clear the
bezel locking nuts, and lift off.
Replacement
Action
Install Right Cover
Steps
Reference
Verification
Action
Steps
Reference
1. Verify that cutouts on front and rear portion of cover are placed
Installation
under locking nuts.
2. Be sure cover is secure against right side of instrument.
Removal
Action
Steps
Remove
Reference
Left Cover
the left and right screws [2] securing the

left side cover [1] to the instrument frame.
2. Slide the left cover to the rear of the

instrument about 1/2-inch to clear the
bezel locking nuts and lift off.
Replacement
Action
Steps
Install Left Cover
Reference
Verification
Action
Steps
Reference
1. Verify that cutouts on front portion of cover are placed under
Installation
locking nuts.
2. Be sure cover is secure against left side of instrument.
Flow Panel (CD18-B1)
Removal
Action
Steps
Prerequisite
1. Verify that the instrument is ON.
Open Upper
Front Cover
Remove Lower
Front Cover
Remove RBC
Aperture Plate
1. Remove Lower Front Cover

(RR-A1.02 Lower Front Cover).
1. From the MAIN MENU, press

[SPECIAL PROTOCOLS] then
[MORE] followed by [DRAIN
BATHS]:
2. Locate the von Behrens transducer
and swivel the red Aperture Plate
Retaining Arm [2] outward.
3. Pull the Aperture Plate [1] straight

out.
Remove WBC
1. Repeat the same procedure to
Reference
Aperture Plate
remove the WBC Aperture Plate.

Note
Note the location of the cutout notch
and WBC etched in the Aperture
Plate.
Replacement
Action
Steps
Reference
Install
Aperture
Note
Plate
The cutout notch [3] in the Aperture Plate

must be facing down and toward the
instrument when the plate is reinserted into
the transducer. Also note the RP etched in
the RBC Aperture Plate and WBC in the
WBC Aperture Plate.
Verification
Action
Verify Background
Counts
Verify Quality
Controls
Steps
1. Run a normal background count cycle and verify results are within acceptable
limits. Refer to Specifications & Checklist.
1. Run three (3) levels of CELL-DYN controls and verify results are within
acceptable assay limits.
Reference
Both the von Behrens RBC and WBC transducers are removed according to the following procedure:
Removal
Action
Prerequisite
Steps
1. If any fluid remains in the

transducers, make sure the
instrument is turned ON.
[MORE] followed by [DRAIN
BATHS].
3. Turn the instrument OFF after

the transducers are drained.
Open Upper Front
Cover
Remove Lower Front
Cover
Remove Top Cover

1. Remove Top Cover (RR-A1.03

Top Cover).
Remove Bezel
1. Remove the bezel (RR-A1.04

Bezel).
Remove PAM PCB
1. Remove the Pre-Amplifier

Module (PAM) PCB (RR-F1.01
PAM (Pre-Amplifier Module)).
Prepare to Remove
1. Route cable connector J3
von Behrens WBC
(connected to PAM) through the
Transducer
Flow Panel.
2. Label and disconnect all

tubing [1] from the von Behrens
WBC Transducer [3].
Reference
Remove von Behrens
1. Using a Phillips-head
WBC Transducer
screwdriver, remove the three

(3) mounting screws [2] from the
transducer holding plate.
2. Cut all cable ties securing the
transducer cable to other
cables/chassis.
3. Route transducer cable away

from front panel and remove
transducer.
Prepare to Remove
1. Route cable connector J4
von Behrens
(connecting to PAM) through the
RBC/PLT Transducer
Flow Panel.
2. Label and disconnect all

tubing [3] from the von Behrens
RBC/PLT Transducer [1].
Remove von Behrens
1. Using a Phillips-head
RBC/PLT Transducer
screwdriver, remove the three

(3) mounting screws [2] from the
transducer holding plate.
2. Lift the transducer assembly [1]

out of the instrument.
Replacement
Action
Steps
Install von Behrens Transducer
Reference
Verification
Action
Verify Gain Settings
Steps
1. Perform VP-09 Signal Processor Module (SPM) Verification/Adjustment.
Reference
Verify Background
Counts
Verify Quality
Controls
WBC Metering PCB Removal
Removal
Action
Prerequisite
Steps
1. Instrument must be in Initialized or

Ready mode.
2. From the MAIN menu, press [SPECIAL
PROTOCOLS] then [MORE] followed
by [DRAIN BATHS]:
3. When the drain baths procedure is

complete, turn OFF the instrument.
Open Upper
Front Cover
Remove Lower
Front Cover

Lower Front Cover).
Remove Bezel
1. Remove the bezel (RR-A1.04 Bezel).
Remove
1. Using a Phillips-head screwdriver,
Plastic Shield
remove the two (2) screws on the

metering PCB transparent plastic
shield.
Disconnect
Metering Tube
Tubing
1. Disconnect the tubing [4] at the top and

bottom of the metering tube [3].
Reference
Caution
When removing tubing, take extreme
care not to break the glass ends. The
metering tube ends are fragile.
2. Use absorbent tissue to remove any

fluid in the metering tube.
Caution
Take extreme care not to allow the
liquid to touch the printed circuit
boards; it can short them out when
power is turned on. If the board does
get wet, make sure it is dry before
turning power back ON.
Remove WBC
1. Using a 1/4 inch nut driver, remove the
Metering PCB
two (2) standoffs [7] on the board.

2. Remove the ground wire [5] on the
bottom standoff.
3. Disconnect cable connector J1 [6] on
the metering PCB.
remove the two (2) mounting screws [2]
securing the metering PCB to the Flow
Panel.
5. Lift the metering PCB [1] off of the Flow

Panel.
RBC/PLT Metering PCB Removal
Removal
Action
Prerequisite
Steps
1. Instrument must be in Initialized or

Ready mode.
2. From the MAIN menu, press [SPECIAL
PROTOCOLS] then [MORE] followed
by [DRAIN BATHS]:
3. When the drain baths procedure is
Reference
complete, place the instrument in

Standby and turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover

Lower Front Cover).
Remove Bezel
Remove
Plastic Shield
remove the two (2) screws on the

metering PCB transparent plastic
shield.
Disconnect
Metering Tube
1. Disconnect the tubing [4] at the top and

bottom of the metering tube [3].
Tubing
Caution
When removing tubing, take extreme
care not to break the glass ends. The
metering tube ends are fragile.
2. Use absorbent tissue to remove any

fluid in the metering tube.
Caution
Take extreme care not to allow the
liquid to touch the printed circuit
boards; it can short them out when
power is turned on. If the board does
get wet, make sure it is dry before
turning power back ON.
Remove
RBC/PLT
Metering PCB
1. Using a 1/4 inch nut driver, remove the

two (2) standoffs [7] on the board.
2. Remove the ground wire [5] on the
bottom standoff.
3. Disconnect the cable connector J1 [2]

on the side of the metering PCB.
Note
The RBC Metering PCB [1] is installed
upside down. Push the metering tube

all the way down to access the top
screw securing the metering board to
the flow panel.
remove the two (2) mounting screws [6]
securing the metering PCB to the Flow
Panel.
5. Lift the metering PCB off of the flow

panel.
Replacement
Action
Install Metering PCB
Steps
Reference

Note
Verify that tubing is not pinched when installing the plastic shield.
Verification
Action
Verify Count
Times
Steps
Reference
1. Verify appropriate count times (see VP-04 Metering System Timing Adjustments RBC and WBC:
o
Verify RBC/PLT count times after installing RBC/PLT metering PCB.
Verify WBC count times after installing WBC metering PCB.
Removal
Action
Prerequisite
Steps

Reference
[MORE] followed by [DRAIN BATHS]:
2. Turn instrument power OFF.

Open Upper
Front Cover
Remove Lower
Front Cover

Remove Bezel
Remove Top
1. Remove Top Cover (RR-A1.03 Top
Cover
Open Top Inner

Cover
Cover).

loosen the two (2) screws [1] securing
the top inner cover [2].
2. Lift the top inner cover upward and to

the right.
Note
This cover is hinged and stands in a
folded back position without support.
Remove Top
Shield from
PAM PCB
the top shield [1] on the PAM PCB [2].
2. Remove the shield.

Route HGB
Cable
1. Disconnect the cable connector from

J2 [3] and route the HGB [7] cable
through the hole [5] at the right side of
the WBC metering PCB [6] and pull it
through the hole.
Remove Tubing Caution

The tubing is stiff. Use care when
removing the tubing to avoid breaking
the HGB Flow Cell.
1. Remove the black tubing from the

T-fitting [2] above and below the HGB
Flow Cell [1].
Note
Use absorbent tissue to capture any
excess liquid in the HGB Flow Cell.
Remove
Hemoglobin
remove the two (2) screws [3]
Flow Cell
securing the HGB flow cell to the flow

panel.
2. Route the HGB cable from behind the

tubing installed at solenoid 2-6 and
remove the HGB Flow Cell module.
Replacement
Action
Steps
Install Hemoglobin Flow Cell
Reference
Verification
Action
Steps
Verify HGB Offset, Gain,
1. Perform HGB Self Test and Adjust HGB Gain (see Pre-Amp Alignments).
Precision
2. Verify HGB precision.
Verify Background
1. Run a normal background count cycle and verify results are within
Counts
Reference
acceptable limits. Refer to Specifications & Checklist.
Verify Quality Controls
Fluid Power Supply (CD18-C1)
Removal
Action
Prerequisite
Steps
1. Place the instrument in Standby and

turn OFF the power.
Remove Top
Cover
Reference
Cover).
Open Top
1. Open Top Inner Cover (RR-B1.04
Inner Cover
Remove
Tubing
Hemoglobin Flow Cell).
Note
Cut any cable ties on cables
surrounding the fluid power supply
assembly as needed.
1. Label and disconnect the tubing from
the T-connector at the top of
solenoid 5-6 [4].
solenoid 5-5 [3].
3. Label and disconnect the silicone
tubing [1] from the top of the 0.5 psi
accumulator located at the rear of the
fluid power supply.
solenoid 5-1 [2].
5. Disconnect the tubing [5] from the
chassis floor fitting [6] next to the left
pump assembly.
6. Disconnect the tubing [7] connected to

the reagent panel waste line (at the rear
of the Reagent Inlet Panel).
Remove
1. Locate solenoid 1-6 and its
Tubing
corresponding silicone tubing [1].
(continued)
Follow the tubing to the back of the

Flow Panel, then label and disconnect
the tubing at the in-line connector.
2. Remove the clamp [2] and plug [3] from

the Vacuum Accumulator drain line [4],
and pull the tubing through the rear of
the Reagent Inlet Panel [5].
Disconnect
Connectors
1. Disconnect cable connector J6 [3] from

the Pump Relay PCB [1].
the Pump Relay PCB [1].
3. Disconnect cable connector J1
(three-pin cable) from the internal waste
sensor PCB located directly under the
Pump Relay PCB.
the Solenoid Driver board.
5. Disconnect cable connector J10 [4]
from the Solenoid Driver board.

(bottom connector) from the Solenoid
Driver Module (SDM).
Remove Fluid
Power Supply

remove the three (3) corner mounting
screws and the one (1) central
mounting screw securing the Fluid
Power Supply to the base of the
chassis.
2. Lift the Fluid Power Supply directly up

and out of the instrument.
Note
Verify that all cable connectors and
tubing are disconnected.
Replacement
Action
Install Fluid Power Supply
Steps
Reference
Verification
Action
Steps
Verify Vacuum, Pressure,
1. Perform VP-03 Vacuum and Pressure Adjustments.
Count Times
2. Verify all count times (see VP-04 Metering System Timing
Reference
Adjustments - RBC and WBC).
Syringe Assembly (CD18-E1)
Removal
Action
Prerequisite
Steps

turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover
Remove Top
Cover
Open Top
Inner Cover
Remove
Connectors

Lower Front Cover).

Cover).

1. Mark and remove the cable connectors

at the top of the cable distribution
board [1]. There are four cable
connectors that must be unplugged
from this board: J20, J21, J22, and
J23.
2. Cut all cable ties securing cables to

other cables (there are approximately
6-8 cable ties).
Reference
Remove Wash
Block Tubing
1. Using a 3/32-inch hex wrench, remove

the clamp securing the aspiration
tubing [3].
2. Using an adjustable wrench, loosen the
screw securing the two (2) clamps [2]
securing the wash block tubing.
3. Label and disconnect the two (2) tubes
from the Wash Block (top is diluent and
bottom is waste).
4. Route the clamps with attached tubing

through the cutout [4] on the left side of
the Sample Probe Driver assembly
chassis [2].
Remove
Sample Probe
1. Disconnect the tubing from the top of

the Sample Probe.
Tubing
Remove
1. Disconnect the cable connectors
Sample Probe
J1 [2] [5] (ribbon) and J2 [3] [6] (power)
Motor Cable
from both the top [1] and bottom [4]

chopper PCBs.
Remove
Sample Probe
remove the two (2) top and two (2)
Assembly
bottom mounting screws [1] securing

the Sample Probe Driver Assembly to
the chassis.
2. Lift the Sample Probe Driver Assembly

out of the instrument.
Note
Before lifting the Sample Probe Driver
Assembly completely out of the
instrument, check to make sure that all
cables are disconnected. Disconnect
any cables that you find still attached,
making sure to mark them before
unplugging.
Replacement
Action
Install Sample Probe Assembly
Steps
Reference
Verification
Action
Verify Normal Operation
Steps
1. Run three (3) cycles and verify normal operation, e.g. binding, liquid
Reference
leaks, etc.
1. Perform VP-12 Sample Probe Alignment Check.
Verify Sample Probe

Alignment
Hazard
Removal
Action
Prerequisite
Steps

turn OFF the power.
Remove Top
Cover
Open Top Inner
Cover).
Cover
Remove Left Side
1. Remove the left side cover (see
Cover
Remove Fan
RR-A1.06 Left Cover).
Note
The fan above the Diluent Syringe
Driver Assembly must be removed
before the driver assembly can be
removed.

remove the two (2) top fan
screws [1].
Note
Screws are secured by a nut. Verify
that ALL nuts are removed prior to
removing fan.
Reference
loosen the two (2) bottom fan

bracket screws [2].
Note
If necessary, rotate the split pin
installed on the directional valve
drive linkage of the Diluent Syringe
to allow the fan to be removed.
3. Move the fan to the rear.
Disconnect
1. Disconnect the tubing [3] on both
Diluent Syringe
sides of the directional valve [2] at
Driver Assembly
the top of the Diluent Syringe [1].

Note
Use absorbent tissue to capture any
excess liquid.
2. Disconnect ribbon cable 9520444
from the back of the bottom chopper
PCB.

from the top chopper PCB.
Remove Diluent
Note
Syringe Driver
Lyse Syringe Driver Assembly [1],
Assembly
Diluent Syringe Driver Assembly [2],

and Sample Syringe Driver
Assembly [3].
1. Using a 7/64-inch hex wrench,
remove the two (2) hex screws [5] on
the front of the base plate [4] located
at the bottom of the diluent driver
assembly [2].
loosen the hex screw [6] from the
rear base plate [4] of the Diluent

Syringe Driver Assembly [2].
3. Slide the Diluent Syringe Driver

Assembly [2] forward, tilt it back, and
remove it through the right side of
the chassis.
Replacement
Action
Steps
Install Diluent Syringe Driver Assembly
Reference
Verification
Action
Steps
Verify Operation
1. Verify VP-10 Diluent and Sample Verification/Adjustment.
Verify Quality
Reference
Controls
RR-E1.04 Sample Syringe Driver Assembly
Removal
Action
Prerequisite
Steps
1. Place the instrument in Standby

and turn OFF the power.
Remove Top
Cover
Open Top Inner

Cover
Remove Left Side

Cover
Remove Fan

Cover).


1. Remove the fan located above the

Diluent Syringe Driver Assembly
(see Remove Fan, RR-E1.02
Diluent Syringe Driver Assembly).
Disconnect
Tubing
1. Disconnect the tubing [2] on top of

the Sample Syringe Driver
Assembly [1].
Reference
Disconnect
1. Cut the cable ties securing the cable
Connectors
harness to the back of the syringe

assembly.

from the chopper driver PCB.
Remove Sample
Note
Syringe Driver
Assembly
Diluent Syringe Driver Assembly [2],

and Sample Syringe Driver
Assembly [3].
remove the two (2) hex screws [5]
on the front base plate [4].
rear base plate [4] of the Sample
3. Slide the Sample Syringe Driver

Assembly [3] forward, tilt it back,
and remove it through the right side
of the chassis.
Replacement
Action
Steps
Reference
Install Sample Syringe Driver Assembly
Verification
Action
Verify Sample
Steps
Reference
1. Verify sample volume (VP-10 Diluent and Sample Verification/Adjustment).
Volume
Verify Quality
Controls
1. Run three (3) levels of CELL-DYN controls and verify results are within acceptable
assay limits.
RR-E1.06 Lyse Syringe Driver Assembly
Removal
Action
Prerequisite
Steps
Reference
Remove Top Cover
1. Remove Top Cover (RR-A1.03

Top Cover).
Open Top Inner
Cover
Remove Left Side
Cover
Disconnect
1. Disconnect the tubing [2] from the
Tubing/Cables
top of the Lyse Syringe [1].

from the chopper driver PCB.
Remove Lyse
Note
Syringe Driver
Assembly
Diluent Syringe Driver

Assembly [2], and Sample Syringe
Driver Assembly [3].
remove the two (2) hex screws [5]
on the front base plate [4].
rear base plate [4] of the Lyse
3. Slide the Lyse Syringe Driver
Assembly forward, tilt it back, and

remove it through the right side of
the chassis.
Replacement
Action
Steps
Reference
Install Lyse Syringe Driver Assembly
Verification
Action
Verify Quality
Controls
Steps
Reference
Electronics / Card Cage (CD18-F1)

RR-F1.01 PAM (Pre-Amplifier Module)
Removal
Action
Prerequisite
Steps

turn OFF the power.
Reference
Open Upper
Front Cover
Remove Lower
Front Cover
Remove Top
Cover
Cover).
Remove Bezel
Flip Bezel Latch
Bracket UP
remove the lower screw [1] on the

bezel latch bracket [2] attached to the
chassis.
2. Loosen, but do not remove the top
screw [3] on the latch.
3. Flip the latch up and to the right.

Remove Top
Shield from
PAM PCB
the top shield [5] on the PAM PCB.
2. Remove the shield.

Remove PAM
Cover

remove the two (2) left retaining
screws [4] [6] on the left side of the
PAM cover plate and loosen the two
(2) right retaining screws [7].
2. Slide the PAM cover [8] to the left and

off the PAM PCB.
Disconnect
Cables
1. Mark and disconnect the five (5) cable

connectors J1 [1], J2 [2], J3 [3],
J4 [4], and J5 [5] from the PAM PCB.
Note
The J3 connector is for WBC and the
J4 connector is for RBC/PLT.
Remove PAM
Board
1. Using a 1/4-inch nut driver, remove

the four (4) metal standoffs [6], on the
corners of the PAM PCB.
2. Lift the PAM PCB off the Flow Panel.
Replacement
Action
Install
Steps
Reference
PAM
Board
Note
Be sure that the metal standoffs securing the PCB in place and the screws securing the
metal cover over the PCB are securely fastened. Failure to properly secure the standoffs
and cover screws may result in electrical noise being induced due to improper grounding.
Verification
Action
Verify PAM Printed Circuit Board
Steps
1. Perform VP-08 Pre-Amplifier Module (PAM) Adjustment.
Adjustments
Verify SPM Printed Circuit Board
Adjustments
1. Perform VP-09 Signal Processor Module (SPM)

Verification/Adjustment.
RR-F1.02 MPM (Motor Processor Module) Board
Reference
Removal
Action
Prerequisite
Steps

turn OFF the power.
Remove Top
Cover
Open Top
Inner Cover
Remove
Cables
Remove MPM
Board

Cover).

1. Label and disconnect cable connectors

(leading to the MPM PCB):
o
J1 [1]
J2 [2]
J3 [3]
J4 [4]
J5 [5]
J6 [6]
J7 [7]
J10 [8]
1. At each corner of the MPM PCB,

compress the plastic snap-in pins and
pull the corner of the PCB free from the
pin.
Note
Repeat this procedure for the snap-in
pin at the center of the PCB.
2. Lift the MPM PCB off the top inner

cover.
Reference
Replacement
Action
Steps
Reference
Install MPM Board
Verification
Action
Steps
Verify Motor Operation
Reference
1. Perform VP-11 Stepper Motor Power Test and Verification.
RR-F1.03 CDM (Cable Distribution Module) Board
Removal
Action
Prerequisite
Steps

turn OFF the power.
Remove Top
Cover
Open Top
Inner Cover
Disconnect
Cables

Cover).

1. Label and disconnect the following

cable connectors (leading to the CDM
PCB):
o
J1 [15]
J2 [14]
J3 [13]
J4 [12]
J5 [11]
Reference
Remove CDM
Board
J6 [10]
J7 [9]
J8 [8]
J9 [7]
J11 [16]
J14 [17]
J15 [18]
J16 [19]
J17 [20]
J18 [21]
J19 [22]
J20 [1]
J21 [2]
J22 [3]
J23 [4]
J31 [5]
J32 [23]
1. At each corner of the CDM PCB,

compress the plastic snap-in pin and
pull the corner of the PCB free from the
pin.
Note
Repeat this procedure for the snap-in
pin at the center of the PCB.
2. Lift the CDM PCB off the top inner

cover.
Replacement
Action
Install CDM Board
Steps
Reference
Verification
Action
Steps
Reference
1. Perform VP-07 Cable Distribution Module Test Points.
Verify CDM PCB

Voltage
1. Run three (3) cycles and verify normal operation, e.g. binding, liquid leaks,
etc.
RR-F1.04 Hard Disk Drive
Removal
Action
Prerequisite
Steps

Open Upper Front
Cover
Remove Lower
Front Cover
Remove Bezel


Bezel).
Remove Right
Side Cover
Remove Front
Card Cage Shield
1. Remove the right side cover

(RR-A1.05 Right Cover).

securing the Front Card Cage
Shield [2] to the chassis.
2. Remove the shield from the

instrument.
Reference
Prepare to
1. Disconnect the Backplane PCB
Remove Card
Power cable from the DC Regulator
Cage from
PCB.
Chassis
2. Disconnect the Disk Drive Power

Cable from the DC Regulator PCB.
3. Remove the two (2) screws securing
the top section of the backplane
PCB shield and remove the shield.
4. Disconnect cable connectors J9 and

J10 on the Backplane PCB.
Remove Card
Cage

remove the four (4) screws [1]
securing the card cage [2] to the
chassis.
Caution
Once the screws are removed, the
card cage drops down. Excessive
movement on the card cage can
damage connectors on the
Backplane PCB.
Remove Hard
Disk Drive
1. Slide the bottom of the card cage

forward and tilt it backward.
Note
Verify that all four (4) screws

securing the hard disk drive to the
card cage are accessible.
securing the hard disk drive [2] to
the card cage.
3. Note the alignment of the red

stripe [5] on the ribbon cable in
relationship to the hard disk drive
connector.
Note
The same alignment must be
maintained during replacement.
4. Disconnect the power [3] and data
cable [4] connectors (rear of hard
disk drive).
5. Remove the hard drive from the

instrument.
Replacement
Action
Steps
Reference
Install Hard Disk Drive
Verification
Action
Steps
Verify Hard Disk Drive Setup
Reference
1. Perform VP-14 Hard Disk Drive Setup and Verification.
RR-F1.05 Floppy Disk Drive
Removal
Action
Prerequisite
Steps

turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover

Remove Bezel
Remove Floppy
Disk Drive
loosen the four (4) screws [1] securing

the floppy drive mounting bracket [2]
to the bezel.
securing the floppy drive to the
mounting bracket [4].
3. Remove the floppy disk drive from the

instrument.
Reference
Replacement
Action
Install Hard Disk Drive
Steps
Reference
Verification
Action
Steps
Verify Floppy Disk Drive
1. Power ON instrument, and allow it to initialize.
Operation
2. During initialization, verify that the LED on the floppy disk drive turns on and
that the LCD display screen is operational.
Reference
RR-F1.06 Signal Processor Module (SPM)
Removal
Action
Prerequisite
Steps

turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover
Remove Bezel
Remove Front
Card Cage
Shield


instrument.
Remove SPM
PCB
Note
Card cage PCBs from left to right:
SPM, CCM, DLA and SBC.
from the SPM PCB [1].
2. Open the top and bottom plastic

mounting tabs.
Note
Reference
Pulls the board slightly out of the card

cage.
3. Pull the board out of the instrument.
Replacement
Action
Install SPM
Steps
Reference
PCB
Note
When reinserting the SPM PCB into its slot at the rear of the card cage, press it firmly
into place to ensure proper seating.
Verification
Action
Verify SPM PCB
Steps
1. Perform VP-09 Signal Processor Module (SPM) Verification/Adjustment.
Adjustments
Note
Make SPM adjustments in the following order:
Verify Background
Counts
RBC
RER
WBC
PLT
Reference
RR-F1.07 Cell Count Module (CCM)
Removal
Action
Prerequisite
Steps

turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover
Remove Bezel
Remove Front
Card Cage
Shield


instrument.
Remove CCM
PCB
Note
1. Disconnect cable connectors J1
through J3, and J5 from the CCM
Reference
PCB.
2. Open the top and bottom plastic

mounting tabs [1].
Note
Pulls the board slightly out of the card
cage.
3. Pull the board out of the instrument.
Replacement
Action
Install CCM
Steps
Reference
PCB
Note
When reinserting the CCM PCB into its slot at the rear of the card cage, press it firmly
Verification
Action
Verify Background
Counts
Verify Quality
Controls
Steps
RR-F1.08 Data Link Adapter (DLA)
Removal
Reference
Action
Prerequisite
Steps

turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover
Remove Bezel
Remove Front
Card Cage
Shield


instrument.
Remove DLA
PCB
Note
1. Disconnect cable connectors J1 [2]
and J2 [3] from the DLA PCB [1].
remove the screw securing the DLA
PCB bracket to the top of the card
cage.
3. Grasp the top and bottom of the DLA

PCB and pull the card from the card
cage and away from the instrument.
Reference
Replacement
Action
Install DCM
Steps
Reference
PCB
Note
When reinserting the DLA PCB into its slot at the rear of the card cage, press it firmly
Verification
Action
Verify Background
Counts
Steps
Reference
RR-F1.09 Single Board Computer (SBC)
Removal
Action
Prerequisite
Steps

turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover

Remove Bezel
Remove Front
Card Cage
Shield

Reference
instrument.
Remove SBC
Note
PCB

1. Label and disconnect all cable
connectors from the SBC PCB [1].
remove the screw securing the SBC
PCB bracket to the top of the card
cage.
3. Grasp the top and bottom of the SBC

PCB and pull the card from the card
cage and away from the instrument.
Replacement
Action
Install SBC
Steps
Reference
PCB
Note
When reinserting the SBC PCB into its slot at the rear of the card cage, press it firmly
Verification
Action
Steps
Reference
Verify Background
Counts
RR-F1.10 Card Cage Backplane PCB
Removal
Action
Prerequisite
Steps

turn OFF the power.
Open Upper
Front Cover
Remove Lower
Front Cover

Remove Bezel
Remove Front
Card Cage
Shield


instrument.
Remove SPM
1. Remove SPM PCB.
PCB
Remove CCM
PCB
1. Remove CCM PCB.
Reference
Remove DLA
1. Remove DLA PCB.
PCB
Remove SBC
1. Remove SBC PCB.
PCB
Remove
1. Using a Phillips-head screwdriver
Backplane PCB
(stubby), remove the top three (3)
Shield
screws [2] securing the Backplane

PCB Shield [1] to the card cage [3].
2. Using a Phillips-head screwdriver
(stubby), remove the bottom three (3)
screws [4] securing the Backplane
PCB Shield [2] to the card cage [3].
3. Disconnect cable connectors J1, J9,
and J10 from the Backplane PCB.

instrument.
Remove
Backplane PCB
1. Verify that all screws and cable

connections are removed from the
Backplane PCB.
2. Remove the Backplane PCB from the

instrument.
Replacement
Action
Steps
Reference
Install Backplane
PCB
Note
When reinserting the backplane PCB into its slot at the rear of the card cage, press
it firmly into place to ensure proper seating.
Verification
Action
Steps
Reference
1. Place instrument in Ready mode.
2. Verify that instrument properly initializes and primes.

Verify Card Cage Backplane
1. Perform VP-06 Card Cage Backplane Test Points.
PCB Voltages
Verify Background Counts
1. Run a normal background count cycle and verify results are within
acceptable limits. Refer to Specifications & Checklist.
1. Run three (3) levels of CELL-DYN Controls and verify results are within
LCD/Keyboard (CD18-G1)
Removal
Action
Prerequisite
Steps

turn OFF the power.
2. Unplug power cord from rear of

instrument.
Open Upper
Front Cover
Remove Lower
Front Cover
Reference
Remove Bezel

Bezel).
Remove LCD
Display Screen
Assembly
1. Place bezel cover face down on a

flat surface.
2. Disconnect cable connector CN2 [3]
from the backlight inverter PCB [4].
3. Release the backlight wires from the
cable clip.
securing the LCD Display Screen
mounting plate to the bezel cover.
securing the LCD Display Screen [6]
to the mounting plate.
6. Disconnect the LVDS cable

connector [7] at the rear of the LCD
Display Screen.
Note
When disconnecting the LVDS cable
connector, pull the connector, NOT
the wires.
Replacement
Action
Install the LCD Display
Steps
Screen Assembly
Note
During replacement, verify that the LCD Display Screen assembly is
centered in the bezel cover, before securing the assembly to the cover.
Reference
Verification
Action
Verify LCD
Display Screen
Installation
Steps
Reference
1. Turn the instrument Power ON and

allow the instrument to initialize.
2. Verify that the LCD Display Screen
is turned on and centered in the
bezel.
3. Verify that the LVDS cable (between

the LCD display [3] and the LCD
Interconnect PCB [1]) is routed
correctly [1-3]
Note
Failure to properly route and secure
the LVDS cable may result in
damage to the wires and connector.
Power Supply (CD18-H1)
Removal
Action
Prerequisite
Steps

Note
Never touch a PCB when the power
is ON.
2. Unplug power cord from rear of

instrument.
Open Upper Front
Cover
Remove Lower
Reference
Front Cover
Remove Top
Cover
Remove Bezel
Cover).

Bezel).
Remove Right
1. Remove the right side cover
Side Cover
Remove Cable
(RR-A1.05 Right Cover).
1. Label and disconnect the four (4)
Connectors from
cable connectors at the rear of the
Power Switch
power switch [1].

Note
The power switch contains two (2)
black and two (2) white cable
connectors.
Remove Cable
1. Label and disconnect cable
Connectors from
connectors J3 through J9, and J11
DC Regulator PCB
from the DC Regulator PCB [2].
Remove Cable
Note
Connectors from
Cut cable ties from cable bundles
AC Regulator PCB
containing cable connector J7.

1. Label and disconnect the cable
connectors J5, J7 and J9 from the
AC Regulator PCB [1].
2. Label and disconnect the chain
cable connector J6 from the AC
Regulator PCB and J2 from the DC
Regulator PCB.
3. Release the chain cable connector
from the cable clamp attached to

the switching power supply.
Remove Power
Supply Assembly

remove the four (4) screws [1] from
the rear of the instrument securing
the power supply assembly to the
instrument chassis.
2. Carefully lift the power supply up,
then partially out of the instrument.
3. Verify that no cable connectors are

connected to the power supply
assembly.
Caution
The power switch is vulnerable to
damage during this process.
4. Remove the power supply

assembly.
Replacement
Action
Steps
Install Power Supply Assembly
Reference
Verification
Action
Verify System Voltages
Steps
1. Perform VP-06 Card Cage Backplane Test Points.
Reference
Verification Procedures
VP-01 Preparation for Alignment and Verification
VP-01 Preparation for Alignment and Verification
Purpose
Preparation for Alignments/Verifications.
Module CELL-DYN 1800
Materials Required See Test Equipment and Supplies Required. Time
Test Equipment and Supplies Required
ITEM
DESCRIPTION
QTY
Digital Volt Meter
5-inch Jumper Leads
Stopwatch
Vacuum Gauge 0-30 Inches
Pressure Gauge 0-5 lb.
Pressure Gauge 0-10 lbs.
Polystyrene Microspheres, 5.0 or 5.01 m
Polystyrene Microspheres, 3.31 m
10
Hemostats
11
500 mL Flask or Beaker
12
Silicone Tubing
AR
01:00 hr
13
Standard Tool Kit
14
Fresh Blood Samples With Reference Values
AR
15
Assayed Controls for CELL-DYN 1800
AR
16
20k Ohm 1% Resistor
17
15k Ohm 1% Resistor
18
10 mL Graduated Cylinder
19
40 L Micropipettes
AR
20
100 L Micropipettes
AR
21
50 mL Counting Cups
AR
22
25 mL Graduated Cylinder
23
12-inch Ruler With 1/16 Increments
24
Oscilloscope (Optional)
AR = As Required
Preparation for Alignment/Verification

Perform the following procedure to prepare the CELL-DYN 1800 System for alignment/verification:
Action
Steps
Check Reagents
1. Verify all reagents are correct and are 1/3 full.
Remove Upper
1. Remove Upper Front Cover (RR-A1.01 Upper Front Cover).
Front Cover
Remove Lower
1. Remove Lower Front Cover (RR-A1.02 Lower Front Cover).
Front Cover
Remove Left
1. Remove Left Cover (RR-A1.06 Left Cover).
Cover
Remove Right
1. Remove Right Cover (RR-A1.05 Right Cover).
Cover
Remove Top
1. Remove Top Cover (RR-A1.03 Top Cover).
Reference
Cover
Clean Aperture
Plates
1. Remove and clean both RBC/PLT and WBC aperture plates following the
procedure in Section 9: Service and Maintenance, Subsection:
Cleaning/Replacing Aperture Plates of the CELL-DYN 1800 Operator's Manual.
Clean HGB Flow

Cell
Re-initialize
1. Clean the HGB Flow Cell following the procedure in Section 9, Subsection:
Cleaning the HGB Flow Cell of the CELL-DYN 1800 Operator's Manual.
1. Re-initialize the instrument by turning the system OFF then ON again.
Instrument
Check Tubing,
1. When Initialization is complete, press [PRIME/RUN] to prime the instrument.
Look For Leaks
Observe the flow system for leaks, tubing placement, pinched tubing, etc.
Install Covers
1. Reinstall the covers on the instrument.
Run Background
1. Run a Background count. Verify all background values are within the following
Count
specifications:
WBC
0.5 K/L
RBC
0.05 M/L
HGB
0.1 g/dL
PLT
10.0 K/L
Record
1. In the MAIN MENU, press [CALIBRATION].
Calibration
2. Record all Calibration Factors for Open and Pre-Dilute modes.
Factors
Record Dilution
Factors
Enter Service
Personnel ID
1. Type 94043 to display the DILUTION FACTORS screen. Record all Dilution
Factors.
1. In the MAIN MENU, type 999 for Operator ID and press Enter on the PC keyboard
or membrane keypad. This is to identify all runs performed by service personnel.
Order of Alignment/Verification
The following procedures are presented to ensure proper alignment of the CELL-DYN 1800 System.
Sequence
Procedure
VP-03 Vacuum and Pressure Adjustments
VP-04 Metering System Timing Adjustments - RBC and WBC
VP-06 Card Cage Backplane Test Points
VP-07 Cable Distribution Module Test Points
VP-08 Pre-Amplifier Module (PAM) Adjustment
VP-09 Signal Processor Module (SPM) Verification/Adjustment
VP-10 Diluent and Sample Verification/Adjustment
VP-11 Stepper Motor Power Test and Verification
VP-12 Sample Probe Alignment Check
When performing adjustments:
At the completion of each Vacuum and Pressure Adjustment procedure, verify the WBC and RBC count times and
normal system operation
Prior to starting any electronic alignment, verify the power supply voltages in VP-06 Card Cage Backplane Test
Points and VP-07 Cable Distribution Module Test Points.
Whenever an alignment adjustment is performed on the Pre-Amplifier Module (PAM), verify all parameters on the
Signal Processor Module (SPM).
VP-02 Decontamination
Purpose
To decontaminate all surfaces before servicing. Module CELL-DYN 1800
Materials Required Sodium hypochorite solution
Action
Decontamination
Ratio
Time
00:15 min
Steps
1. The OSHA Bloodborne Pathogen Rule (29 CFR Part 1910.1030) requires the
decontamination of laboratory equipment prior to servicing or shipment:
o
Decontaminate the instrument by performing the Auto-Clean cycle. This

cycle flushes all of the fluid pathways with reagents to purge any waste from
the fluid pathways. The Open Mode Sample Probe is automatically rinsed
Reference
after every cycle. The surfaces of the instrument should be wiped with a
nonabrasive detergent solution to remove any soiling, then wiped with a
tuberculocidal disinfectant, such as a 0.5% sodium hypochlorite solution.
2. To calculate the percent (%) sodium hypochlorite concentration desired, use the
following formula:
a. A = Percent (%) of sodium hypochlorite solution desired
b. B = Percent (%) of sodium hypochlorite stock solution (as purchased)
c. X = Parts of water to be mixed with one part of the sodium hypochlorite stock
solution:
d. Example: If you need a 0.5% solution of sodium hypochlorite for a cleaning

procedure, and the label on the bottle of bleach states that it is 5.25%
sodium hypochlorite, then:
e. Add 9.5 parts deionized water to 1 part bleach to obtain a 0.5% sodium
hypochlorite solution, or 9.5 mL of deionized water to 0.5 mL of bleach
(5.25% sodium hypochlorite) to obtain 10 mL of a 0.5% solution of sodium
hypochlorite.
3. If the instrument is to be shipped, it must be decontaminated prior to shipment. This

is accomplished by pressing the [CLEAN FOR SHIPPING] key in the SPECIAL
PROTOCOLS menu. Instructions for this procedure are given in the
CELL-DYN 1800 Operator's Manual, (Section 9 Preparing the Instrument for
Extended Periods of Non-Use or Shipping).
VP-03 Vacuum and Pressure Adjustments
Purpose
Align and adjust pressure and vacuum. Module CELL-DYN 1800
Materials Required Digital Volt Meter (DVM)

0-5 psi Pressure Gauge
0-30 psi Pressure Gauge
0-30 HG Vacuum Gauge
Phillips Screwdriver
Thin Flat Blade Screwdriver
Time
00:30 min
This procedure consist of four parts (perform in sequence):
Regulator Alignment
Pressure Adjustment (0.5 psi)
Pressure Verification (High)
Vacuum Adjustment (8 inch)
The CELL-DYN 1800 System utilizes one vacuum and two pressure levels to accomplish the following tasks: moving sample,
reagents, and waste, bubble mixing of sample, and back flushing RBC and WBC apertures. The vacuum and bubble mix
pressures are adjustable by a solid-state regulator. The backflush pressure is not critical and the pump is under direct
computer control.
The solid state regulator has two input ports: P1 for pressure and P2 for vacuum. It also has jumper terminals which
accommodate all desired vacuum and pressure ranges. The jumper positions are:
Jumpers Pressure (psi) Vacuum (inches Hg)
A-B
0.0 - 3.0
0.0 - 6.0
C-D
2.5 - 5.1
5.0 - 10.2
E-F
4.3 - 6.6
8.6 - 13.2
G-H
6.0 - 8.0
12.0 - 16.0
Regulator Alignment
Action
Access Pump
Relay Board
Steps
Note
This offset adjustment must be performed with no pressure or vacuum applied to
the regulator.
1. Disconnect the appropriate pumps on the Pump Relay Board and bleed off
Reference
pressure from the Accumulators.

o
J4 for low pressure
J2 for vacuum on new-style pumps
2. Remove the pressure or vacuum line from the top of the regulator.
Measure Voltage
1. On the Pump Relay Board the voltage should be 5.0 0.15 volts at J7 pin 7 for
vacuum, and J8 pin 5 for pressure.
Note
You must remove the board mounting screws and free the rest of the board to
make the remaining adjustments. Disconnect J6 or J7 while relocating the
appropriate printed circuit board to avoid shorting out components.
2. Note the current position of jumper E1 and set E1 to the C-D position.
3. Connect the DVM (Digitial Volt Meter) negative lead to TP3 (GND) on regulator
board. Connect the positive lead to TP2 (REF). The voltage should be
1.00V 0.14 volts.
o
If voltage is not correct, re-check Step 1.
If voltage is still not correct, replace the regulator.
4. Connect the DVM negative lead to TP1 and the positive lead to TP2, and read the
voltage. Adjust R18 for a voltage of 0.000 0.005.
Note
If voltage is negative, turn R18 (offset) clockwise; if the voltage is positive, turn R18
counterclockwise.
Reposition E1
1. Move jumper E1 back to the proper operating position.
Reconnect
1. Reconnect the pressure or vacuum line to the top of the regulator and reconnect
Pressure / Vacuum
the cable(s) on the pump relay module.
Line
Pressure Adjustment (0.5 psi)

Follow the steps below for pressure adjustment:
Action
Remove Top
Cover
Steps
Reference
Open Top Inner
1. Open Top Inner Cover (RR-B1.04 Hemoglobin Flow Cell).
Cover
Connect
Pressure Gauge
1. Locate the small silicone tubing connected to the in-line fitting at top of the 0.5 psi
Pressure Accumulator (located closest to the rear of the instrument).
2. Connect a 0-5 psi gauge in-line with the silicone tubing and fitting (the Pressure
Accumulator is located on the part of the Fluid Power Supply which is closest to the
rear of the instrument).
Adjust
1. Adjust R16 on the Regulator board for 0.55 psi 0.02.
Regulator
Note
Clockwise adjustment increases pressure and counterclockwise adjustment
decreases pressure.
2. Remove the gauge and reconnect the silicone tubing to in-line connector.
Pressure Verification (High)

Follow the steps below for pressure verification:
Action
Locate In-line
Connector
Steps
1. Locate solenoid valve 1-6 and trace the tubing back through the flow panel to the
in-line connector.
Connect Pressure
1. Connect a 0-30 psi gauge in-line.
Gauge
2. In the RUN screen, press [CLEAR ORIFICE] while observing the gauge.
Verify Correct
1. When the pump activates, verify a pressure of no less than 4 psi.
Pressure
If the pressure is less than 4 psi, check for leaks. Replace the Pressure
Pump if necessary. (Refer to RR-C1.01 Fluid Power Supply.)
Vacuum Adjustment (8 inch)

Follow the steps below for solid state regulator adjustment:
Reference
Action
Locate Vacuum
Steps
Reference
Note
This is a coarse adjustment only. The fine adjustment is predicated upon metering
Regulator
system count times. Make this coarse adjustment only if there is reason to believe that
the vacuum is grossly misadjusted.
1. Locate the solid state vacuum regulator on the rear side of the Fluid Power Supply
(closest to the flow panel).
Adjust R16
1. Remove the TYGON tubing from the top of the Vacuum Accumulator (located on the
back of the Fluid Power Supply closest to the flow panel) and connect a 0-30" Hg
gauge in-line between the tubing and the Accumulator.
2. Adjust R16 (accessible only from the front of the Fluid Power Supply) for
8.25" Hg 0.25".
Note
Clockwise adjustment increases vacuum and counterclockwise adjustment decreases
vacuum.
Reconnect
1. Once R16 is adjusted, remove the vacuum gauge and reconnect the tubing line to the
Tubing Line
regulator.
VP-04 Metering System Timing Adjustments - RBC and WBC
Purpose
Adjust Metering System to regulate count times. Module CELL-DYN 1800
Materials Required Phillips Screwdriver
This procedure consist of two parts:
Links
RBC Metering System Timing Adjustment

WBC Metering System Timing Adjustment
Time
00:15 min
The instrument uses the Volumetric Metering process to regulate the count cycle and to ensure that a precise volume of
sample is analyzed for the measurement. This table lists the results of a fault report displayed on the screen if a flow error or
clog occurs during a run cycle.
Metering Timing Fault Report
Detector Masked Flow Error Time Valid Meniscus Time

(in seconds)
(in seconds)
Clog Time
(in seconds)
(in seconds)
WBC Upper
0 - 0.5
0.5 - 1.0
1.0 - 3.0
3.0+
WBC Lower
0 - 3.5
3.5 - 4.0
4.0 - 6.5
6.5+ or greater than the moving average
RBC/PLT Upper 0 - 3.0
3.0 - 4.0
4.0 - 6.0
6.0+
RBC/PLT Lower 0 - 5.5
5.5 - 6.0
6.0 - 7.5
7.5+ or greater than the moving average
This figure illustrates the timing relationships for WBC and RBC measurements.
CELL-DYN 1800 Metering Timing Chart (in Seconds)
For each transducer there are two distinct counting periods, T1 and T2. RBC Metering Tube and WBC Metering Tube
illustrate the counting periods for the RBC and WBC metering tubes, respectively.
RBC Metering Tube
WBC Metering Tube
All count times are based on the vacuum level of the 8-inch vacuum reservoir and tubing length. These count times are
critical in that all clog and flow system alarms are generated via these count times. The vacuum adjustment (Vacuum
Adjustment (8 inch)) sets the coarse vacuum requirement, but further adjustments are necessary to ensure proper timing for
the flow system alerts.
RBC Metering System Timing Adjustment
Follow the steps below for adjusting the timing of the RBC metering system:
Action
Verify
Steps
1. Ensure that the RBC aperture plate has been removed, cleaned, and reinstalled following
Count
the procedure in Section 9: Service and Maintenance, Subsection: Cleaning/Replacing
Time
Aperture Plates of the CELL-DYN 1800 Operator's Manual.
2. Run a background count and verify that the RBC displayed count time (T2) is 6.7 seconds
0.2 seconds.
o
If the specification is met, go to Step 4.
If the specification is not met, go to Step 3.
3. Readjust the vacuum to correct the count time if the time is out of specification (refer to
Adjust R16, Step 2 of the Vacuum Adjustment (8-inch) procedure previously discussed).
4. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].
5. Verify the time displayed for RBC UPTIME is 4.8 to 5.2 seconds. (This time, displayed in
milliseconds, represents T1, which is the time when the RBC valve 1-2 opens until the
meniscus reaches the upper detector.)
Reference
If the specification is met, the procedure is complete.
If the specification is not met, go to Step 6.
6. Adjust the metering tube.

o
Raise the metering tube to increase the upper time.
Lower the metering tube to decrease the upper time.
7. Repeat Step 2 through Step 5 until the RBC Up Time/Count Time is within specification.
WBC Metering System Timing Adjustment

Follow the steps below for adjusting the timing of the WBC metering system:
Action
Prerequisite
Steps
Note
The RBC count times (T1 & T2) must be within specification before performing this
procedure.
Verify Count
Time
1. Remove and clean the WBC aperture plate as described in Section 9: Service and
Maintenance, Subsection: Cleaning/Replacing Aperture Plates of the
CELL-DYN 1800 Operator's Manual.
2. Run a background count and verify the count time displayed to the right of the WBC
histogram is 5.0 0.5 seconds.
Note
There is no count time adjustment procedure.
3. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].
4. Verify that the time displayed for WBC UPTIME is 1.8 to 2.2 seconds. (This time
represents T1 which is the time when the WBC valve 4-3 opens until the meniscus
reaches the upper detector. See WBC Metering Tube.)
o
If the WBC UPTIME specification is met, the procedure is complete,
If the WBC UPTIME specification is not met, go to Step 5.
5. Adjust the metering tube.

o
Raise the metering tube to increase the upper time.
Lower the metering tube to decrease the upper time.
6. Repeat Step 1 through Step 4 until WBC UPTIME/COUNT TIME is within specification.
Reference
VP-05 CMOS Setup Verification/Adjustment
Purpose
Provide instructions to verify and/or modify CMOS settings. Module CELL-DYN 1800
Materials Required None
Time
Action
00:10 min
Steps
Preparation
1. Power OFF the system.
Access CMOS Setup
1. Power ON the analyzer and press the Delete key, when prompted, to display
Utility Screen
the CMOS Setup Utility Screen.
1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.
2. Press the Enter key.
3. Refer to the figure (H2_6006) and make any necessary adjustments to the
Standard CMOS Setup screen.
Verify/Modify
Standard CMOS
Setup
Note
Press the up/down arrow keys to select a setting and then press PgUp/PgDn
keys to modify.
4. Press ESC to exit the screen.
Reference
1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.
3. Refer to the figure and make any necessary adjustments to the Advanced
CMOS Setup screen.
Note
keys to modify.
Verify/Modify
Advanced CMOS
Setup
1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.
3. Refer to the figure and make any necessary adjustments to the Advanced
Chipset Setup screen.
Note
keys to modify.
Verify/Modify
Advanced Chipset
Setup
Note
The SAMSUNG LCD assembly is Type 8, while the TOSHIBA LCD assembly is Type 7.
1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.
3. Refer to the figure and make any necessary adjustments to the Power
Management Setup screen.
Note
Verify/Modify Power
Management Setup
keys to modify.
1. Use the up/down arrow keys to select the PERIPHERAL SETUP.

3. Refer to the figure and make any necessary adjustments to the Peripheral
Setup screen.
Note
keys to modify.
Verify/Modify
Peripheral Setup
1. Press F10.
Save CMOS Settings

and Exit CMOS Setup
The message Save current settings and exit (Y/N)? displays.
2. Type Y and press Enter.

Note
Saves the CMOS settings, exits the CMOS Setup Utility and restarts the
computer.
Verification
1. Verify that the system boots up properly to the application software.
VP-06 Card Cage Backplane Test Points
Purpose
Identify test points on Backplane PCB. Module CELL-DYN 1800
Time
00:10 min
The following table lists the test points located on the backplane board and the figure illustrates the test points on the
backplane board.
Test
Function
TP1 +5V
Range
+5.00V 0.20V
TP2 +12V Analog
+12V 0.50V
TP3 -12V Analog
-12V 0.50V
TP4 +12V Digital
+12V 0.50V
TP5 -12V Digital
-12V 0.50V
Card Cage Backplane (Rear Side)
Ripple
Card Cage Backplane (Card Side)
LD4 +12VDC (DIG)
LD2 -12VDC (ANALOG)
LD5 -12VDC (DIG)
LD1 +5VDC
LD3 +12VDC (ANALOG)
Note
LEDs indicate voltages supplied to the board.
VP-07 Cable Distribution Module Test Points
Purpose
Identify test points on CDM. Module CELL-DYN 1800
Time
00:10 min
The following table lists the test points located on the Cable Distribution Module (CDM).
Location
Pin
Function Voltage Ripple

(J5 and J8)
Brown
Ground
< 0.05V
N/A
Blank
N/A
N/A
N/A
Orange
Ground
< 0.05V
N/A
Yellow
+14V
0.5V
N/A
Green
+28V
1.00V
N/A
Blank
N/A
N/A
N/A
Note
On the CDM, +28V is used to initially energize solenoids and +14V is used to keep them closed.
VP-08 Pre-Amplifier Module (PAM) Adjustment
Purpose
Adjust HGB self test and gain.
Adjust RBC/PLT and WBC aperture currents.

Time
00:40 min
Flat Blade Screwdriver

5-inch Jumper Leads
20K Ohm 1% Resistor
15K Ohm 1% Resistor
The Pre-Amplifier Module (PAM) is located on the upper right corner of the Flow Panel. Hemoglobin circuitry, and the
RBC/PLT and WBC aperture currents require verification and/or adjustment on this module. The HGB flow cell should be
cleaned before performing hemoglobin alignments (refer to Section 9, Subsection: Cleaning the HGB Flow Cell in the
CELL-DYN 1800 Operator's Manual).
Pre-Amplifier Module (PAM) Test Points
The following table lists the test points located on the PAM.
Location
Function
Voltage
TP5
Analog Ground
N/A
TP4
+100V
TP7
HGB Self Test/Gain Adjust +1.84V 0.02V (Self Test)
+100V 0.5V
+5.2V 0.2V (Gain Adjust)
Test points for the PAM are shown in the figure below.
Pre-Amp Module (PAM) Test Points
Follow the steps below to align the PAM:
Action
Steps
Reference
Open Upper Front Cover
Remove Lower Front Cover
1. Remove Lower Front Cover (RR-A1.02 Lower Front Cover).
Remove Top Cover
Remove Bezel
Remove Pre-amp Cover
1. Remove the pre-amp cover (RR-F1.01 PAM (Pre-Amplifier Module)).
HGB Self Test
1. Remove jumper JMP3 (lower right corner).

2. Press the touch plate to cycle the instrument and fill the HGB flow cell
with fresh reagent.
3. Connect the positive lead of the DVM to TP7.
4. Connect the ground of the DVM to TP5.
5. Adjust R14 to 1.84 volts 0.02 volts.
6. Verify the voltage at TP7.
7. Connect jumper JMP3 and verify voltage at TP7 returns to 5 volts.

Note
If voltage remains unchanged, the jumper may be in the wrong position.
Adjust HGB Gain
with fresh reagent.
3. Adjust R4 (HGB Gain Adjust) for 5.2 volts 0.2 volts.
with fresh reagent.

Note
If voltage does not meet specification, repeat Step 2 and Step 3.
Verify +100 Volts
1. Connect the positive lead of the DVM to TP4, and the negative lead to
TP5 (Gnd).
2. Verify 100 volts 0.5 volts.

Adjust RBC/PLT Aperture
1. Disconnect the RBC signal cable from J4.
Current
2. Connect a 20K Ohm 1/4 watt resistor across pins 4 and 5 of J4.
3. Connect the DVM across the resistor.
4. Run a background count.
5. Adjust R72 (RBC/PLT aperture current adjust) for 11.0 volts 0.05 volts.
6. Remove the resistor and reconnect cable to J4.

Adjust WBC Aperture
1. Disconnect the WBC signal cable from J3.
Current
2. Connect a 15K Ohm 1/4 watt resistor across pins 4 and 5 of J3.
3. Connect the DVM across resistor.
4. Run a background count.
5. Adjust R66 (WBC aperture current adjust) for 12.0 volts 0.05 volts.
6. Remove the resistor and reconnect cable to J3.

1. Reinstall the top cover, upper and lower front covers, bezel and pre-amp
Install Covers
cover.
The following table contains the specifications for the Pre-Amp Module alignment.
Pre-Amp Alignments
HGB SELF TEST
TP7
R14 +1.84V 0.02 V
HGB GAIN
TP7
R4
5.2 V. 0.2 V
RBC/PLT APERTURE CURRENT 20K Ohm Resistor R72 11.0 V. 0.05 V
WBC APERTURE CURRENT
15K Ohm Resistor R66 12.0 V. 0.05 V
VP-09 Signal Processor Module (SPM) Verification/Adjustment
Purpose
Adjust WBC, RBC, PLT Gain. and RBC Edit Ratio (RER) percentage (%). Module CELL-DYN 1800

Thin Flat Blade Screwdriver
Polystyrene Microspheres, 5.0 or 5.01 m
Polystyrene Microspheres, 3.31 m
Clean 50 mL Container
Note
Time
00:50 min
The Signal Processor Module (SPM) is located in the main electronics card cage. Alignment of the WBC, RBC, and
PLT gains are critical adjustments that must be verified and/or adjusted before instrument accuracy can be
established. It is important to maintain the adjustment order for this verification procedure. The adjustment order is:
Sequence
Activity
RBC Gain
RER Adjustment
WBC Gain
PLT Gain
Latex particles are used to perform these adjustments. The particles must be mixed vigorously before diluting to obtain
accurate results.
The Gain and RBC Cell Editing adjustments are performed in the Gain Adjust Mode, which allows multiple counts to be run
on the same sample. When Gain Adjust Mode is entered, whatever is in the Pre-Mix Cup is transferred to the WBC Cup and
5 mL of diluent is left undisturbed in the RBC Cup.
When performing RBC or PLT adjustments, 10 mL of diluent must be placed in Pre-Mix Cup before entering Gain Adjust
Mode to prevent air from being pulled into the von Behrens WBC Transducer during a count cycle.
Test points for the SPM are shown in the figure below:
SPM Test Points
RBC Gain
Action
Prepare Latex
Dilution
Steps
1. Pour 10 mL of diluent into the Pre-Mix Cup and add 1 drop of well-mixed 5.0 latex
particle solution to the 5 mL of diluent already in the RBC Mixing Chamber.
2. From the RUN screen, press [SPECIMEN TYPE].
3. Press Shift and # (simultaneously) to activate the Gain Adjust Mode.

Note
The message <PREPARING FOR GAIN ADJUST.> displays and WBC dilution is
transferred to the WBC Mixing Chamber. When the preparation cycle is complete,
the RUN screen displays.
Set RBC Gain
1. Press the Touch Plate to run a cycle.

Note
Always return to the RUN screen to run a cycle.
Note
When the procedure is completed, or if there is a need to exit the Gain Adjust Mode
while performing this procedure, such as constant clogs or improper dilution ratio,
press [SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
Note
The RBC count should be between 3.5 and 4.5. If the count is outside this range,
modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].
3. Press [SMOOTHING OFF].

Note
The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing
function is ON.
4. Press [RBC HISTOGRAM].
5. Observe the lower part of the histogram data to determine if the channel number for
peak of 100 is Channel 98 1 channel.
o
If the specification is met, go to RER Adjustment.
If the specification is not met, adjust R27.

Note
Turning R27 clockwise decreases the channel number; turning R27

counterclockwise increases the channel number.
Reference
6. Return to the RUN screen and run another cycle.
7. Observe the channel number for peak count of 100.

o
If the gain is still outside of specification, repeat this process until the peak
count meets the specification (100 is in Channel 98 1 channel). Refer to
RBC Gain Adjustments (Example)
Note
Turning R27 clockwise decreases the channel number; turning R27

counterclockwise increases the channel number.
Continue with
1. Go to RER Adjustment.
RER Adjustment
RBC Gain Adjustments (Example)
RER Adjustment
Action
Determine
Steps
1. Verify that the dilution level on both the WBC
Adequate
and RBC transducers (left side) is above the
Dilution Amount
aperture (orifice) plates.

o
If the dilution level in transducers is

met, go to Determine Edit Ratio
Percentage.
If the specification is not met, go to

Step 2.
2. From the RUN screen, press [SPECIMEN

TYPE], then [PATIENT SPECIMEN].
3. Press the touch plate to run a cycle (exits Gain
Adjust Mode).
4. Pour 10 mL of diluent into the Pre-Mix Cup and
add 1 drop of well-mixed 5.0 m latex particle
solution to the 5 mL of diluent already in the
RBC Mixing Chamber.
5. In the RUN screen, press [SPECIMEN TYPE].
6. Press Shift and # (simultaneously) to activate

the Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJ.>
displays and diluent in the Pre-Mix Cup is
transferred to the WBC Mixing Chamber. When
the preparation cycle is complete, the RUN
screen displays.
Determine Edit
Ratio
1. Press the Touch Plate to run a cycle. Make a

note of the RBC results.
Percentage
Note
The RBC count should be between 3.5 and 4.5.
If the count is outside this range, adjust the
dilution ratio of the latex particles by adding
more diluent or latex to the RBC Mixing
Chamber.
2. Repeat Step 1 two more times for a total of
three (3) runs. Calculate the average of the
three RBC counts.
3. Refer to Cell Edit Table, to find the target value

for the following:
Reference
a. Use the average RBC calculated in

Step 2 to determine the Edit Ratio
Percentage that coincides with the
calculated average.
Note
Example: A count of 4 million relates to an edit
ratio of 28.0% 1.3.
Adjust RER
Cell Edit Table
Note
Use the edit ratio calculated in Determine Edit
Ratio Percentage above to perform the
following procedure:
1. From the MAIN Menu, press [DIAGNOSTICS]
followed by [RAW DATA].
2. Observe the RBC RER displayed on the screen

from the last cycle.
o
If the specification is met (see Cell Edit

Table), go to WBC Gain.
If the specification is not met, go to

Step 3.
3. Adjust R51.
Note
Turning R51 clockwise decreases the RER
percentage; turning R51 counterclockwise
increases the RER percentage.
4. Return to the MAIN Menu and run another
cycle.
5. Observe the RBC RER in the RAW DATA
screen.
6. If the ratio is still outside of specification, repeat
this process until the RBC Editing Ratio is within
the specified value.
7. Return to the MAIN Menu and press [RUN] to
access the RUN screen.
8. Observe the RBC Histogram.
9. The trailing edge of the histogram should be

straight with almost no humps. Refer to Editing
Ratio Displays.
Continue with
1. Go to WBC Gain.
WBC Gain
Editing Ratio Displays
WBC Gain
Action
Steps
Reference
Prepare Latex
1. From the [RUN] screen, press [SPECIMEN TYPE], then [PATIENT SPECIMEN].
Dilution
2. Press the touch plate to run a cycle (exits Gain Adjust Mode).
3. Obtain a clean container.
4. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice to
display the [10 mL DISPENSE] key.
5. Press [10 mL DISPENSE] four times to dispense 20 mL of diluent into the container.
Note
Each 10 mL dispense requires key to be pressed twice.
6. Add 1 drop of well mixed 5.0 m latex particle solution into the 20 mL of diluent and mix
well.
7. Before entering the Gain Adjust Mode, use the prepared WBC latex dilution to fill the
Pre-Mix cup up to the level of the diluent inlet port.
8. From the MAIN Menu, press [RUN] followed by [SPECIMEN TYPE].
9. Press Shift and # (simultaneously) to activate the Gain Adjust Mode.

Note
The message <PREPARING FOR GAIN ADJ.> displays and WBC dilution is
transferred to the WBC Mixing Chamber. When the preparation cycle is complete, the
RUN screen displays.
Set WBC
Gain
Note
Always return to the RUN screen to run a cycle.
Note
When the procedure is completed, or if there is a need to exit the Gain Adjust Mode
while performing this procedure, such as constant clogs or improper dilution ratio, press
[SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
Note
The WBC count should be between 10.0 and 30.0. If the count is outside this range,
modify the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
2. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].

Note
The key changes to [SMOOTHING ON] and is highlighted, indicating the smoothing
function is ON.
4. Press [WBC HISTOGRAM].
5. Observe the lower part of the histogram data to determine if the channel number for
peak of 100 is Channel 56 1 channel.
o
If the specification is met, go to PLT Gain.
If the specification is not met, adjust R22, and repeat Step 1 through Step 5.
Note
Turning R22 clockwise decreases the channel number; turning R22 counterclockwise
increases the channel number.
Continue with
1. Go to PLT Gain.
PLT Gain
WBC Gain Adjustments
PLT Gain
Action
Prepare
Latex
Steps
1. From the [RUN] screen, press [SPECIMEN TYPE],

then [PATIENT SPECIMEN].
Reference
Note
The PLT channel cannot be correctly
2. Press the touch plate to run a cycle (exits Gain Adjust

Mode).
3. From the MAIN Menu, press [SPECIAL

PROTOCOLS] followed by [MORE] twice. Place a
clean container under the Sample Probe and press
[10 mL DISPENSE] four times to dispense 20 mL of
diluent.
Note
Each 10 mL dispense requires key to be pressed
twice.
4. Add one (1) drop of well-mixed 3.31 m latex particle
solution and mix well.
5. Hold the diluted latex solution under the Sample Probe
and press [1/50 DILUTION] to aspirate for 1:50
dilution, while in the SPECIAL PROTOCOLS Menu.
6. Place a clean container under the Sample Probe and
press [1/50 DISPENSE] to dispense the 1:50 dilution.
7. Pull open Solenoid 2-5 (located directly under the left
side of the RBC/PLT Transducer) to drain the existing
solution in the RBC Mixing Chamber.
8. Pour the 1:50 dilution of 3.31 latex particles into RBC

Mixing Chamber.
Enter Gain
Adjust
Mode
1. Pour 10 mL of diluent into the Pre-Mix Cup (do not add

any latex particle solution).
2. From the RUN screen, press [SPECIMEN TYPE].
3. Press Shift and # (simultaneously) to activate the

Gain Adjust Mode.
Note
The message <PREPARING FOR GAIN ADJ.>
displays and WBC dilution is transferred to the WBC
Mixing Chamber. When the preparation cycle is
complete, the RUN screen displays.
Note
When running PLT counts, the lower front cover must
be on the instrument to prevent noise from interfering
with PLT results.
Adjust PLT
Gain
Note
adjusted unless the RBC channel is at

the correct gain. The RBC channel
must be verified prior to adjusting the
PLT channel.
The PLT count should be between 150 and 350. If the

count is outside this range, adjust the dilution ratio of
the latex particles by adding more dilution or latex.
2. From the MAIN Menu, press [DIAGNOSTICS]
followed by [MORE].

Note
The key changes to [SMOOTHING ON] and is
highlighted, indicating the smoothing function is ON.
4. Press [PLT HISTOGRAM].
5. Observe the lower part of the histogram data to

determine if the channel number for peak of 100 is
Channel 136 1 channel (refer to PLT Gain
Adjustments (Example).).
o
If the specification is met, the procedure is

complete.
If the specification is not met, adjust R41.

Note
Turning R41 clockwise decreases the channel

number; turning R41 counterclockwise increases the
channel number.
6. Return to the RUN screen and run another cycle.
7. Repeat Step 2 through Step 5.
8. To exit Gain Adjust mode, go to the RUN Menu and
press [SPECIMEN TYPE] followed by [PATIENT
SPECIMEN]
9. Run two to three background cycles.
10.Verify acceptable background results (refer to

Specifications & Checklist).
PLT Gain Adjustments (Example)
VP-10 Diluent and Sample Verification/Adjustment
Purpose
Verify diluent dispense to maintain proper dilution ratios. Module CELL-DYN 1800
Materials Required 10 mL Graduated Cylinder
Time
00:15 min
40 L Micropipettes
100 L Micropipettes
Clean 50 mL Container
WARNING
Potential Biohazard
This procedure consist of both Diluent and Sample adjustment that can be accessed with the following links:
Diluent Volume Verification

Sample Volume Verification
To minimize problems like coincidence passage, the CELL-DYN 1800 uses two different dilution ratios of whole blood to
diluent. The ratio for WBC/HGB is 1:285; the ratio for RBC/MCV/PLT is 1:12,801. The following procedure is used to verify
the diluent dispense to maintain proper dilution ratios and thereby optimize instrument performance.
Diluent Volume Verification
Action
Steps
Verify
1. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.
Diluent
2. Place an empty 10 mL graduated cylinder under the Sample Probe and press [10 mL
Volume
Reference
DISPENSE] twice to dispense 10 mL of diluent.

3. Verify a volume of 10 mL 0.2 mL.
4. Place an empty 10 mL graduated cylinder under Sample Probe and press [1/50
DILUTION]. Once the probe has returned to the aspirate position, press
[1/50 DISPENSE] to dispense.
5. Verify a volume of 5 mL 0.1 mL.

Note
The volume dispensed is under direct computer control. If the volume is out-of-range, the
Dispenser and Stepper Motor drive circuitry must be repaired. (Refer to RR-E1.02
Diluent Syringe Driver Assembly.)
Sample Volume Verification
Action
Steps
Sample Volume
1. Remove the 1/32" silicone tubing attached to the top of the sample probe.
Verification
2. Attach a 100 microliter pipette to the silicone tubing.
3. Place the tip of the pipette on the bottom of a small container and press [10 mL
DISPENSE] twice.
Note
Keep the tip of the pipette submerged when dispensing.
Reference
4. Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
5. Press [1/50 DILUTION].
6. Verify that the column of liquid is no more than 1/16 inch above or below 100
microliter mark on the pipette.
7. Replace the 100 microliter pipette with a 40 microliter pipette.
8. Place the tip of the 40 microliter pipette on the bottom of a small container and press
[1/50 DISPENSE].
9. Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
10. Press [1/250 DILUTION].
11.Verify that the column of liquid is no more than 1/16" above or below 40 microliter
mark on the pipette.
Remove Pipette
1. Place a waste container under the tip of the pipette and press [1/250 DISPENSE].
2. Remove the pipette and re-attach the silicone tubing to the sample probe.
Note
The volume aspirated is under direct computer control. If the volume is out-of-range,
the Sample Syringe and Stepper Motor Drive must be repaired. (Refer to RR-E1.04
Sample Syringe Driver Assembly.)
VP-11 Stepper Motor Power Test and Verification
Purpose
Verify stepper motor power. Module CELL-DYN 1800
Materials Required None
Action
Test Stepper
Motor Power
Time
00:30 min
Steps
1. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times.
Then press [SERVICE DEC CODE].
2. Type in 128 from the keyboard and press Enter.
3. The test runs for approximately 30 seconds.
4. When the test is complete, the results are displayed on the screen.
5. Compare the results displayed on the screen with the nominal values listed in Motor
Reference
Power Specifications. Ensure that all values fall within the specified ranges.
Motor Power Specifications
VP-12 Sample Probe Alignment Check
Purpose
Check Probe Alignment.
Materials Required Standard Set of Hex Wrenches Time
Action
Check
Steps
1. From the MAIN Menu, press
Probe
[DIAGNOSTICS], followed by [MORE]
Alignment
twice, then [PROBE HOME].
2. Observe the wash block in relationship

to the probe tip. Verify that the tip of the
probe is between the waste removal
channel and the top of the countersink,
located at the bottom of the wash block.
Note
Proper probe tip location is essential for
proper cleaning of the probe, and
prevents carryover and CV problems.
3. If alignment is required, loosen the set
screw on the probe collar and raise or
lower probe until proper height is
00:10 min
Reference
achieved.
4. Retighten set screw on probe collar.
5. Press PROBE DOWN to exit.
VP-13 Serial Transmit to LIS Verification
Purpose
Verify operation of serial port.
Materials Required Laptop computer w/Windows 2000

Time
00:40 min
Copy of CELL-DYN 1800 Interface Specification (Latest Revision)

Nine (9) Pin Null Modem Serial Cable
This verification procedure is used to verify the operation of the serial port (COM 1) that is used for communication with a
Laboratory Information System (LIS). This procedure only verifies the transmit function.
A 9 pin, "null modem", serial cable is needed for this procedure.
Note
The screens shown in this procedure are given for example only. Each model instrument has slightly different field
structure and content to the count data message that is downloaded. Consult the applicable interface specification
document for details.
Action
Create New
Connection
Steps
1. Place the laptop near the instrument and

connect the null modem serial cable to
COM 1 on the right side of the instrument.
Reference
Connect the other end to COM 1 on the

laptop.
2. From the Windows 2000 desktop, use the
mouse to click on Start.
3. Highlight Programs, Accessories,

Communications, and click Hyper
Terminal.
The Connection Description window
displays.
4. In the Name field, type in the filename
sertest1.
5. Select the first icon and click the OK

button.
Select
1. The Connect To window displays:
Connection
2. At the bottom, in the box Connect

using:, use the mouse to select COM 1.
Note
All the other boxes are blocked (gray).
3. Click the OK button.
Configure
1. The COM1 Properties window displays:
COM1
2. Configure the Bits per second: field to

match the Baud Rate in the CELL-DYN
SETUP menu.
3. Verify that the Data bits, Parity and Stop
bits are configured according to the
CELL-DYN SETUP menu.
4. In the CELL-DYN SETUP menu, verify
the CTS enabled setting:

o
If CTS enabled is off set Flow

control: to Xon/Xoff.
If CTS enabled is on set Flow

control: to Hardware.
5. Click the OK button.
sertest1
Active
1. The test session window named sertest1

is now active.
Note
If the message "Unable to open COM1.
Please check your port settings"
displays, there may be another device
assigned to the COM1 port, such as the
modem. Contact the local area IS
administrator for assistance with resolving
device interrupt conflicts.
2. Compare the results to the interface

specification. The screen is shown for
example only. Transmit specimen data as
many times as desired.
Transmit
Data
1. From the MAIN Menu, press

[DATALOG], followed by [TRANSMIT
DATA].
2. Enter starting and ending sequence
numbers of data to be transmitted.
3. Compare the results to the
CELL-DYN 1800 Interface Specification.

Note
The screen below is shown for example
only.
4. If desired, click on File then Print to

obtain a printout. When testing is
complete, click on File then Save. After
saving, click on Call, then Disconnect,
then exit the window by clicking the X.
Close out the Hyper Terminal window by
clicking the X.
VP-14 Hard Disk Drive Setup and Verification
Purpose
Provide instructions to verify/modify CMOS Settings, partition and format
replacement hard disk drives

Materials
DK, Drive, Hard, IDE (8200570701)
Required
DKS (C) SRV ABBOTT CD3200 (8938070001)
Time
00:30 min
CELL-DYN 1800 Installation Disk

Action
Preparation
Steps
Note
If replacing an existing customer hard disk drive, print copies of all customer
settings, quality control data files, and the data log if the drive is accessible. It
may not be possible to access this data if the hard disk drive is corrupt or
defective.
1. Power OFF the system.
2. Verify jumper setting is set to Master according to Jumper Configuration Table

on hard disk drive.
Install Hard Drive
1. Install the new hard drive using the existing hardware and cables (refer to
RR-F1.04 Hard Disk Drive).
Reference
Access CMOS Setup

Utility Screen
Verify CMOS Settings:

Standard CMOS Setup
1. Power ON the analyzer and press the Delete key, when prompted, to display
the CMOS Setup Utility Screen.
1. Use the up/down arrow keys to select the STANDARD CMOS SETUP.
3. Set Pri Master to Auto.
4. Set 32 Bit Mode to ON.

Note
Pri Slave, Sec Master and Sec Slave show Not Installed.

1. Use the up/down arrow keys to select the ADVANCED CMOS SETUP.
Advanced CMOS Setup

3. Set Quick Boot to Enabled.
4. Set 1st Boot Device to Floppy.
5. Set 2nd Boot Device to IDE-0.
6. Set 3rd Boot Device to Disabled.
7. Set 4th Boot Device to Disabled.

1. Use the up/down arrow keys to select the ADVANCED CHIPSET SETUP.
Advanced Chipset
Setup
3. Set LCD CRT Selection to Simultaneous.

4. Set LCD Type to Type 8.

1. Use the up/down arrow keys to select the POWER MANAGEMENT SETUP.
Power Management
Setup
3. Set Power Management/APM to Disabled.

4. Set CPU Critical Temperature to 60C/140F.

Peripheral Setup
1. Use the up/down arrow keys to select the PERIPHERAL SETUP.

3. Set OnBoard FDC to Enabled.

Save CMOS Settings
and Exit CMOS Setup
1. Place the DKS (C) SRV ABBOTT CD3200 (8938070001) (service disk) into
the floppy drive.
2. Press F10.
The message Save current settings and exit (Y/N)? displays.
3. Type Y and press Enter.

Note
Saves the CMOS settings, exits the CMOS Setup Utility and restarts the
computer.
Create DOS Partition
1. At the A:\> prompt, type FDISK and press Enter.

2. At the FDISK Options menu, enter choice 1 and press Enter.
3. At the Create DOS Partition or Logical DOS Drive menu enter choice 1 and
press Enter.
Note
If you get the message Primary DOS Partition already exists, press Esc to
exit. Then go to Format Hard Drive, Step 1.
The message Do you wish to use the maximum available size for a
Primary DOS Partition and make the partition active (Y/N)? displays.
4. Type Y, and press Enter.

Note
Uses the maximum size for the hard drive DOS partition and makes it active.
5. When the screen displays System will now restart. Insert DOS system
diskette in drive A:. Press any key when ready, press Enter.
The system reboots and displays the A:\ prompt.
Format Hard Drive
1. At the A:\> prompt, type FORMAT C: /S and press Enter.

The message WARNING: ALL DATA ON NON-REMOVABLE DISK DRIVE
C: WILL BE LOST! Proceed with Format (Y/N)? displays.
2. Type Y, and press Enter.

The message Volume label (11 characters, ENTER for none)? displays.
3. Press Enter for None.

Install DOS and System
1. At the A:\> prompt, type INSTALL and press Enter.
Software
Note
Installs DOS and system files.

2. When the system installation is complete, remove the service disk and insert
the CELL-DYN 1800 Installation disk into the floppy drive.
3. Type Install and press Enter.

Note
When the installation is complete, the message "Power off the instrument
and remove diskette. Power on the instrument." displays.
4. Remove the CELL-DYN 1800 Installation disk and power OFF the instrument.
Verification
1. Power ON the instrument
2. Verify that the system boots up properly to the application software.

Note
During initial power up after a hard disk drive installation, DO NOT turn off the
instrument while the Data Log is being created. This corrupts the Data Log.
3. If printouts are available, re-enter the data from the printouts. If not, perform
instrument calibration procedure and verify control recovery.
VP-15 Software Installation/Upgrades
Purpose
Install or Upgrade Software
Materials Required Disk, installation (8938144001) Time
Action
00:10 min
Steps
Software
Installation/Upgrade
2. Press ESC on the PC keyboard. A warning message displays on the screen.

3. Press Alt and x (simultaneously). The following DOS prompt displays:
C:\CD1800>.
4. Insert the CELL-DYN 1800 Installation disk into Drive A.
5. Type a: and press Enter.
Reference
6. Type install and press Enter.

Note
When the installation is complete, the message "Power off the instrument
and remove diskette. Power on the instrument." displays.
7. Remove the diskette and power OFF the instrument.
8. Power ON the instrument and allow it to initialize.

Note
During initial power up after a hard disk drive installation, DO NOT turn off the
instrument while the Data Log is being created. This corrupts the Data Log.

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Order of Alignment/Verification...................................................................................................................................... 156

Flow Panel System [3]

Fluid Power Supply [2]

Reagent Inlet Panel [6]

Syringe Drive Assemblies [7]

Electronics Card Cage [8]

LCD Display System [4]

Touchpad (Membrane) Keyboard [5]

Power Supply Assembly [1]

Data Interface and Control Subsystem

Solenoid Motor and Pump Subsystem

Single Board Computer Subsystem

AC and DC Power Distribution Subsystem

Data Interface and Control Subsystem

DLA/CCM (Data Link Adapter/Cell Count Module)

CCM/SPM (Cell Count Module/Sample Processor Module)

CCM/CDM (Cell Count Module/Cable Distribution Module)

CCM/MPM (Cell Count Module/Motor Processor Module)

The WBC signal is amplified (gain).

The RBC signal is routed to the cell editing circuitry.

Single Board Computer Subsystem

The Single Board Computer subsystem consists of the following components:

Single Board Computer PCB

Data Link Adapter

Input/Output Ports (serial/parallel)

Keyboards (PC and membrane)

LCD Display Screen

Disk Drives (hard and floppy)

SBC (Single Board Computer) PCB

Receives measurement and analyzer status data from the CCM

Transfers commands from the SBC to the CCM

Sends control data from the UIC to the CCM.

Serial I/O Ports

COM1 RS-232 Data Output

Parallel I/O Port

Size: 8.5 x 6.4 inches (10.4 inches measured diagonally)

Pixels: 640 width x 480 height (or 800 x 600)

Backlight on/off control (software controlled screen saver)

Floppy Disk Drive

Pre-Amplifier Module (PAM)

Signal Processor Module (SPM)

Cell Count Module (CCM)

Cable Distribution Module (CDM)

Solenoid Driver Module (SDM)

Motor Processor Module (MPM)

Stepper Drive Printed Circuit Board (SDP)

Pressure/Vacuum Regulator Module (PVRM)

Power Supply Assembly

Pump Relay Module (PRM)

Single Board Computer (SBC)

Data Link Adapter (DLA)

Pre-amplifier Module (PAM)

The PAM performs the following functions:

Amplifies the initial RBC/PLT, WBC and HGB signals.

A threshold detector signals to the EPLD the presence of valid pulses.

RBC and PLT Signals

Cell Count Module (CCM)

System Clock and Microprocessor Description

16 bit address bus

8 bit data bus

7 - 8 bit multi-purpose I/O ports (CCM and SBC ports)