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TRNG I HC DC H NI
NGHIN CU KH NNG TO
BACTERIOCIN CA VI KHUN
LACTOBACILLUS ACIDOPHILUS
H NI 2013
B Y T
TRNG I HC DC H NI
NGHIN CU KH NNG TO
BACTERIOCIN CA VI KHUN
LACTOBACILLUS ACIDOPHILUS
KHA LUN TT NGHIP DC S
H NI 2013
LI CM N
Trc ht, em xin gi li cm n su sc ti DS. L Ngc Khnh, ngi
trc tip hng dn v to mi iu kin thun li em c th hon
thnh kha lun ny.
Em xin chn thnh cm n TS. m Thanh Xun ng gp nhiu
kin qu bu v tn tnh gip em thc hin ti.
Em cng xin cm n s quan tm, gip ca cc thy c gio, cc anh
ch k thut vin B mn Cng nghip Dc i vi em trong sut qu trnh
nghin cu v lm thc nghim ti b mn.
Cui cng, em xin by t lng bit n gia nh, thy c, bn b, nhng
ngi lun ng vin, gip em trong hc tp v trong cuc sng.
Em xin trn trng cm n!
MC LC
1.1.1. Vi nt v lch s
1.1.2. Phn b
11
11
1.2.7. ng dng
13
16
PHP NGHIN CU
2.1. Nguyn vt liu, thit b
16
16
2.1.2. Ha cht
16
2.1.3. Mi trng
16
17
18
18
18
18
18
19
20
20
21
bacteriocin
2.3.6. Phng php kho st nh hng ca nhit n hot tnh
22
bacteriocin
2.3.7. Phng php kho st kh nng chit bacteriocin bng
22
(NH4)2SO4
Chng 3. THC NGHIM, KT QU V BN LUN
23
23
23
26
28
28
31
34
39
Collection)
BC (Bacterial Cellulose)
Cellulose vi khun
Dha
Dehydroalanine
Dhb
Dehydrobutyrine
Administration)
phm Hoa K
GC
Guanine v Cytosine
Safe)
kDa
kiloDalton
Vi khun lactic
Lan
Lanthionine
MeLan
MethylLanthionine
MRS
MRSA (Methicillin-Resistant
Staphylococcus aureus)
PMF (Proton Motive Force)
ng lc proton
sp. (species)
Loi
subsp. (subspecies)
Phn loi
VRE (Vancomycin-Resistant
Enterococci)
UV (Ultraviolet)
Tia cc tm
DANH MC CC BNG
STT
Tn bng
Trang
1.1
2.1
16
2.2
Cc my mc s dng trong ti
17
3.1
24
26
30
32
35
DANH MC CC HNH V, TH
STT
Tn hnh
Trang
1.1
26
26
31
33
36
T VN
Vic pht hin ra cc cht khng sinh nh du mt bc tin cch mng
ca loi ngi trong cuc chin chng li cc bnh nhim khun, cng ngh
khng sinh c nh gi l mt trong nhng thnh tu khoa hc ln nht ca
th k XX [52]. Tuy nhin, bc vo th k XXI, con ngi ang phi i mt
vi k nguyn hu khng sinh vi s thiu ht cc nhm khng sinh mi trong
khi cc chng vi khun khng thuc khng ngng gia tng [13]. Trc tnh
hnh , vic nghin cu v pht trin nhng hp cht mi c tc dng khng
khun gim s ph thuc vo khng sinh l ht sc cn thit. Bacteriocin
chnh l mt trong s [52].
Bacteriocin l nhng peptid hay protein c tc dng dit khun do vi
khun sinh ra [34]. Chng c pht hin t u th k XX nhng mi
c nghin cu rng ri trong mt vi thp nin tr li y khi nhu cu v
nhng hp cht khng khun nhm thay th khng sinh v cc cht bo qun
ha hc tr nn cp thit [26]. Vi c tnh an ton c FDA xc nhn, vi
khun lactic (LAB) l mt nhm vi khun sinh bacteriocin thu ht s quan
tm c bit ca cc nh khoa hc [52]. Nhiu cng trnh nghin cu
chng minh tc dng c ch vi khun c hi ca cc bacteriocin sinh ra bi
LAB [20]. Nisin mt bacteriocin ca Lactococcus lactis spp. c s
dng rng ri trn th gii nh mt cht bo qun ngun gc sinh hc an ton
v hiu qu [23]. Thnh cng ca nisin bc u cho thy tim nng ng
dng to ln ca bacteriocin t LAB [22].
Ti Vit Nam, bacteriocin ca LAB bt u c nghin cu trong
nhng nm gn y, tuy nhin s lng nghin cu cha nhiu v tp trung
ch yu trong nhm ngnh thc phm v th y. Vi mong mun gp phn
nh vo nghin cu bacteriocin ca LAB Vit Nam, ti Nghin cu
II
III
c im
- Cn gi l cc lantibiotic
- Peptid nh (thng < 5 kDa)
- Bn nhit
- Bin i mnh sau dch m, dn ti s to thnh
cc acid amin him gp: Lan, MeLan, Dha, Dhb
- Chia lm 2 typ: typ A v typ B
- Peptid nh (thng < 10 kDa)
- Bn nhit
- t bin i sau dch m, khng cha Lan
- Chia lm 4 phn lp: IIa, IIb, IIc, IId
- Protein kch thc ln (thng > 30 kDa)
- Km bn nhit
V d
Nisin, lactocin S,
epidermin
Pediocin PA-1,
lactacin F,
enterocin AS48
Helveticin J,
millericin,
enterolysin
10
b. Ph tc dng
Ph tc dng ca cc bacteriocin nhn chung rt khc nhau (Ph lc 2).
Cn c ph tc dng, c th chia bacteriocin ca LAB lm 2 nhm. Nhm th
nht l cc bacteriocin ch c tc dng trn cc loi gn gi vi loi sinh ra
n, v d nh lactobin A sinh ra bi Lactobacillus amylovorus ch c ch mt
s Lactobacillus sp. gn gi. Nhm th 2, t gp hn, c ph tc dng rng, v
d nh nisin sinh ra bi mt s dng Lactococcus lactis subsp. lactis c kh
nng c ch nhiu vi khun Gram (+). Ni chung, cc bacteriocin ca LAB
thng t th hin hot tnh trn vi khun Gram (-) [54].
Bacteriocin c th c tc dng dit khun hoc km khun trn cc chng
vi khun nhy cm, mc tc dng khc nhau do nh hng bi nhiu yu
t nh nng , mc tinh sch bacteriocin, cc iu kin thc nghim
[17]
c. C ch tc dng
C ch tc dng ca bacteriocin rt a dng, mt bacteriocin c th c
nhiu hn mt c ch tn cng t bo ch [52]. Mt s c ch c
xut: thay i hot tnh enzym ni bo, c ch bo t ny mm, to l trn
mng t bo Nhn chung, lp v t bo c coi l ch tc dng chnh ca
cc bacteriocin t LAB. Bacteriocin ph v tnh ton vn ca lp v t bo vi
khun nhy cm, gy mt cc cht ni bo, tiu hao PMF, cui cng gy cht
t bo [54].
C ch tc dng c th ca bacteriocin tng lp l khc nhau. Cc
bacteriocin lp I (lantibiotic) c ch tng hp vch t bo v / hoc to l trn
mng t bo bng cch gn vo lipid II [52]. Cc bacteriocin lp II c cu trc
xon c v lng thn nn c th xen vo mng t bo ch, t to l, kh
cc v lm cht t bo [18]. C ch tc dng ca cc bacteriocin lp III ni
chung cha r [54]; mt s tc gi cho rng l cc lysin tiu khun hay tiu
11
12
13
14
15
kt [57]. Mt liu duy nht mutacin B-Ny266 tim mng bng vo chut
nhim S. aureus nhy cm vi methicillin cho t l t vong l 0%, trong khi t
l t vong nhm chut i chng (khng dng mutacin B-Ny266) l t 70
n 100% [47].
Mc d vn cn thm nhiu nghin cu c th a bacteriocin vo
ng dng trn thc tin lm sng nhng nhng kt qu trn cho thy tim
nng to ln ca bacteriocin trong vic iu tr cc bnh nhim khun [27].
Trong tng lai, s dng bacteriocin ha hn s l mt liu php an ton v
hiu qu thay th cc khng sinh truyn thng [59].
b. Trong cng ngh thc phm
Bacteriocin ca LAB c nghin cu ng dng lm cht bo qun
trong cng ngh thc phm bi c nhiu u im: c ch hiu qu nhiu vi
khun gy hng v gy bnh trong thc phm (in hnh l Listeria
monocytogenes) [34], thn thin vi mi trng v sc khe con ngi [54].
Chng c s dng di 2 dng: trc tip hoc gin tip [20]. Dng trc tip
l cc bacteriocin c tinh ch, hin nay mi ch c nisin v pediocin PA1 c dng rng ri lm cht bo qun thc phm vi cc tn thng mi
nh Nisaplin, ALTA 2341 [20] [52] [54] Dng gin tip l cc LAB c
dng lm ging khi ng (starter culture) hoc b sung vo cc thc phm
ln men, bacteriocin c chng sinh ra s pht huy tc dng khng khun,
gip tng tui th v tnh an ton cho sn phm [20]. tng hiu qu khng
khun, bacteriocin c th c s dng kt hp vi cc phng php khc
nh to mi trng acid, b sung NaCl[34]
16
Xut x
Ha cht
Xut x
Acid lactic
Trung Quc
MgSO4.7H2O
Trung Quc
Amoni citrat
Trung Quc
MnSO4.H2O
Trung Quc
Cao nm men
NaCl
Trung Quc
Cao tht
NaOH
Trung Quc
Ct thy tinh
Trung Quc
Natri acetat
Trung Quc
Ch th mu
Trung Quc
(NH4)2SO4
Trung Quc
Glucose
Trung Quc
Pepton
HCl 1N
Trung Quc
Thch
Vit Nam
K2HPO4
Trung Quc
Xanh methylen
Trung Quc
KH2PO4
Trung Quc
2.1.3. Mi trng
a. Cng thc mi trng MRS lng
17
Pepton
: 1,0 g
Amoni citrat
: 0,2 g
Cao tht
: 1,0 g
Natri acetat
: 0,5 g
Cao nm men
: 0,5 g
MgSO4.7H2O
: 0,02 g
Glucose
: 2,0 g
MnSO4.H2O
: 0,004 g
K2HPO4
: 0,2 g
Nc my va
: 100 ml
: 1,0 g
Cao tht
: 0,5 g
NaCl
: 0,5 g
Thch
: 2,3 g
Nc my va : 100 ml
2.1.4. My mc, dng c
a. My mc
Bng 2.2: Cc my mc s dng trong ti
Tn my
Xut x
Tn my
Xut x
c (Rotofix)
Bp cch thy
Trung Quc
My li tm
Cn k thut
c (Sartorius)
My li tm cao c (Universal)
tc
Cn phn tch
c (Sartorius)
L vi sng
Hn Quc (Daewoo) T m
My o vng v
Ty Ban Nha
khun
(IUL)
My khuy t
Nht (Sanyo)
My lc Vortex
c (IKA)
T lnh
Hn Quc (LG)
Knh hin vi
M (Labomed)
T sy
c (Memmert)
b. Dng c
c (Memmert)
Nht (Sanyo)
18
ng nghim
Chy ci s
Pipet Pasteur
Cc c m
n cn
a petri ng knh 9 cm
Que cy u trn
ng ong
19
20
21
22
23
24
Acid lactic
pH 4
Acid lactic
pH 4
Ln 1
16,0
(-)
18,7
(-)
Ln 2
16,2
(-)
19,8
(-)
Ln 3
17,7
(-)
22,5
(-)
Trung bnh
16,6
20,3
Nhn xt v bn lun:
Kt qu bng 3.1 cho thy: trong c 3 ln th nghim, dch ln men ca
L. acidophilus ATCC 4653 u to c vng c ch trn cc chng vi khun
kim nh B. subtilis ATCC 6633 v E. coli ATCC 25922. Mu trng l dung
dch acid lactic c pH 4 (tng ng pH dch ln men) khng to vng c
ch trong tt c cc th nghim tng ng, iu ny cho php loi tr tc dng
ca acid hu c c trong dch ln men. T , c th rt ra 2 kt lun sau:
Mt l, bacteriocin c trong dch ln men ca L. acidophilus ATCC
4653. Kt qu ny ph hp vi nhiu nghin cu c v bacteriocin ca L.
acidophilus [24] [39] [49] [67] [72]. Trong cc nghin cu ny, cc tc gi
u s dng dch ln men ca vi khun thu nhn bacteriocin.
25
26
nghim
Dch ph t
Dch ph t
bo
NaCl 0,9%
bo
NaCl 0,9%
Ln 1
(-)
(-)
(-)
(-)
Ln 2
(-)
(-)
(-)
(-)
Ln 3
(-)
(-)
(-)
(-)
27
Nhn xt v bn lun:
Kt qu bng 3.2 cho thy: trong c 3 ln th nghim, dch ph t bo
ca L. acidophilus ATCC 4653 u khng to vng c ch trn cc chng vi
khun kim nh B. subtilis ATCC 6633 v E. coli ATCC 25922. Mt khc,
theo kt qu mc 3.1.1, bacteriocin ca L. acidophilus ATCC 4653 c hot
tnh trn 2 chng vi khun kim nh ny. V vy, c th s b kt lun
bacteriocin khng c trong dch ni bo ca L. acidophilus ATCC 4653.
Theo Tagg v cng s, c im mi trng l yu t quyt nh t l cc
dng tn ti ca bacteriocin vi khun Gram (+) [66]. Trong ti ny, L.
acidophilus ATCC 4653 c nui cy trong mi trng MRS lng 37oC
trong 18 gi v khng kim sot pH mi trng trong qu trnh nui cy.
Khng loi tr kh nng bacteriocin ca L. acidophilus ATCC 4653 c th
tn ti dng ni bo nu thay i iu kin nui cy vi khun.
Phng php x l sinh khi c thc hin trong ti l nghin hn
hp sinh khi t ca L. acidophilus trn vi ct thy tinh bng chy ci s
(mc 2.3.3). y l mt phng php ph t bo thch hp vi qui m phng
th nghim [4]. u im ca phng php ny l n gin, d thc hin,
khng yu cu ha cht, thit b t tin, khng c nguy c gy nh hng ti
hot tnh bacteriocin nh mt s phng php khc. Tuy nhin, vi phng
php nghin, t bo vi khun mi c ph v v mt c hc. Khng loi tr
kh nng phng php ny c th cha thch hp gii phng bacteriocin t
sinh khi.
Trong phm vi ti ny, do cha c iu kin th nghim cc iu kin
nui cy v cc phng php x l sinh khi khc, vic xc nh bacteriocin
trong sinh khi ca L. acidophilus ATCC 4653 tm dng li vic kt lun
bacteriocin khng c trong dch ni bo ca vi khun.
28
29
30
Ln th
nghim
pH 2
pH 4
pH 6
pH 8
Ln 1
17,6
16,5
11,7
9,3
Ln 2
18,5
16,8
11,2
9,7
Ln 3
20,2
18,1
12,1
10,2
Trung bnh
18,8
17,1
11,7
9,7
(%)
9,0
37,8
48,4
31
trng pH cao.
20
18.8
17.1
18
16
14
11.7
12
9.7
10
8
6
4
2
0
2
pH
32
trnh ch bin thc phm yu cu phi s dng nhit [62]. V vy, bacteriocin
ca chng L. acidophilus th nghim c nghin cu v nh hng ca
nhit n hot tnh bacteriocin.
Mc ch:
nh gi bn nhit ca bacteriocin sinh ra bi L. acidophilus ATCC
4653.
Cch tin hnh:
Thu dch bacteriocin th (dch ln men) tng t mc 3.2.1. Kho st
nh hng ca nhit n hot tnh bacteriocin theo phng php nu
mc 2.3.6, trong cc gi tr nhit c nghin cu l 70oC, 80oC, 90oC
v 100oC. Vi khun kim nh c s dng l B. subtilis ATCC 6633. Th
nghim c lp li 3 ln trn 3 m nui cy c lp ca L. acidophilus
ATCC 4653.
Kt qu:
Kt qu nh gi hot tnh bacteriocin ca L. acidophilus ATCC 4653
sau khi c x l nhit c th hin trong bng 3.4.
Bng 3.4: Hot tnh bacteriocin ca L. acidophilus ATCC 4653 sau khi c
x l nhit trn vi khun kim nh B. subtilis ATCC 6633
Ln th
nghim
70oC
80oC
90oC
100oC
Ln 1
18,1
18,7
19,4
19,4
19,3
Ln 2
18,7
18,4
18,0
18,3
18,3
Ln 3
16,3
16,7
16,2
16,1
16,3
Trung bnh
17,7
17,9
17,9
17,9
18,0
33
20
17.7
18
17.9
17.9
17.9
18.0
16
14
12
10
8
6
4
2
0
Khngx
xl un 70oC
70oC/ / un
un80oC
80oC// un
un 90oC
90oC// un
un100oC
100oC//
Khng
lnhit
nhit
30
30 pht
30 pht
pht
30 pht
pht
30 pht
30
Hnh 3.4: nh hng ca nhit n hot tnh bacteriocin ca L.
acidophilus ATCC 4653
Nhn xt v bn lun:
Kt qu bng 3.4 cho thy sau khi x l nhit dch ln men cc gi tr
nhit nghin cu, hot tnh bacteriocin ca L. acidophilus ATCC 4653 hu
nh khng thay i. C th, ng knh vng c ch trung bnh qua 3 ln th
nghim sau khi un dch ln men ca chng L. acidophilus th nghim cc
nhit 70oC, 80oC, 90oC, 100oC trong 30 pht u xp x nhau v xp x vi
ng knh vng c ch trung bnh khi dch ln men khng c x l nhit
(17,7mm). T , c th nhn nh bacteriocin ca L. acidophilus c tnh bn
nhit.
Trong cc bacteriocin ca LAB, tnh bn nhit l c tnh c bn ca lp
I v lp II bao gm cc bacteriocin c cu trc l cc peptid phn t lng
34
35
Nng
(NH4)2SO4 (%)
Phn ta
20
Phn dch
Khng to ta
30
13,2
16,6
40
13,7
15,3
50
13,9
14,6
60
14,7
14,2
70
15,6
13,7
80
Nhn xt v bn lun:
Kt qu bng 3.5 cho thy, trong dy nng (NH4)2SO4 nghin cu (t
20 n 80%), nng mui thp nht cho php tch c ta bacteriocin t
36
18
16
14
12
10
Phn ta
Phn dch
6
4
2
0
30
40
50
60
Nng (NH4)2SO4 (%)
70
Hnh 3.5: S thay i hot tnh bacteriocin ca phn ta v phn dch theo
nng (NH4)2SO4 s dng
37
38
39
1. Kt lun:
ti hon thnh c cc mc tiu ra v thu c mt s kt qu
nh sau:
- Trong cc iu kin thc nghim ca ti, bacteriocin ca chng L.
acidophilus ATCC 4653 c tm thy c trong dch ln men v khng
c trong sinh khi. Bacteriocin ny th hin tc dng c ch trn c vi
khun Gram (+) (B. subtilis ATCC 6633) v vi khun Gram (-) (E. coli
ATCC 25922). Trn B. subtilis, bacteriocin to vng c ch trong, r
hn v c ng knh nh hn so vi trn E. coli. ng knh vng c
ch trung bnh ca 3 ln th nghim trn B. subtilis l 16,6mm, cn trn
E. coli l 20,3mm.
- Nhn nh s b mt s tnh cht ca bacteriocin sinh ra bi chng L.
acidophilus ATCC 4653:
Bacteriocin a pH acid, hot tnh tng khi pH gim. Trong cc
gi tr pH nghin cu, bacteriocin th hin hot tnh cao nht
pH 2 vi ng knh vng c ch trung bnh qua 3 ln th
nghim l 18,8mm.
Bacteriocin c tnh bn nhit. Sau khi un 100oC trong 30 pht,
hot tnh bacteriocin hu nh khng thay i.
Bacteriocin c th c chit t dch ln men bng phng php
kt ta (NH4)2SO4 vi nng mui t 30% n 70% (khi
lng/th tch), lng bacteriocin kt ta tng dn khi tng lng
(NH4)2SO4 s dng. nng (NH4)2SO4 70%, phn ta
bacteriocin to vng c ch c ng knh 15,6mm; phn dch
sau khi loi ta bacteriocin vn to vng c ch c ng knh
40
Ting Vit
1. Nguyn Th , Hoa Th Minh T, L Thanh Bnh (2008), Mt s tnh
cht ca bacteriocin c tng hp bi vi khun lactic phn lp t sa b
ti, Tp ch Khoa hc v Cng ngh, 46(6), 33-41.
2. Phan Th Khnh Hoa, Nguyn Vit Cng, L Thanh Bnh (2001), nh
hng ca mt s ngun khong v nit ln sinh trng v sinh tng hp
nisin ca Lactococcus lactis subsp. lactis, Tp ch Khoa hc v Cng ngh,
39(5), 37-43.
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bng phng php ln men bi t bo Lactococcus lactis c nh trn cht
mang Cellulose vi khun (BC) v ng dng trong bo qun tht ti s ch ti
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Linh, Th Huyn, L Vn Trng, Trng Nam Hi (2008), Tng hp v
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uses
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calcium
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nisin, Antonie
van
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PH LC
Ph lc 1: Phn loi s b cc bacteriocin v c im ca mt s bacteriocin tiu
biu cho mi nhm [26]
(A)
Proteinbacteriocin
Vi khun Gram
(-)
Escherichia coli
Lp
Phn
lp
Tn
Khi lng
phn t
(kDa)
C ch tc
dng
Colicin
A, E1 E9,
K, L, N, S4,
U, Y
B, D, H, Ia,
Ib, M, 5, 10
Pyocin R2
Pyocin S1,
S2, AP41
Pyocin F
Alveicin A, B
40 n 80
Nuclease /
To l
40 n 80
Nuclease /
To l
To l
Ging phage
408/358 aa
Ging phage
To l
Klebicin C, D
96
Nuclease
Serracin
Serracin P
66
Ging phage
Glynericin
Glynericin A
50
Ging phage
669
Ging phage
68/76
Ging phage
B
Pseudomonas
aeruginosa
Pyocin
Hafnia alvei
Alveicin
Klebsiella
pneumonia
Serratia
plymithicum
Xanthomonas
campestris
Yersinia
enterocolitica
Erwinia
carotovora
Vi khun Gram
(+)
Lactobacillus
helveticus
Streptococcus
milleri
Enterococcus
faecalis
Staphylococcus
aureus
Klebicin
Typ R
Typ S
Typ F
Ging
colicin
Ging
colicin
Enterocoliticin
Carotovoricin
Carotovoricin
Er
270 aa
75/84/
94
Helveticin J
III
37,5
Cha r
Millericin
III
30
Enterolysin
III
34,5
Lysostaphin
III
25
Thy phn
peptidoglycan
Thy phn
peptidoglycan
Thy phn
peptidoglycan
(B)
Lp
Phn
lp
Tn
Khi lng
phn t
(kDa)
C ch tc
dng
Microcin
Microcin
B17
Microcin V
Microcin
E492
3,1
Enzym ni
bo
To l
To l
Nisin
Lacticin 481
Mersacidin
Pediocin
Plantaricin
E/F
Carnocyclin
A
Lactococcin
A
3,5
3
2
4,6
3,5/3,7
To l
To l
5,8
To l
5,8
To l
Patellamides
0,7
Peptidbacteriocin
Vi khun Gram
(-)
Escherichia coli
II
IIa
IIb
Typ A
A1
A2
8,8
7,9
Vi khun Gram
(+)
I
(Hay
lantibiotic)
II
Typ B
IIa
IIb
IIc
IId
Cyanobacteria
Prochloron
didemni
Ging
microcin
To l
To l
Lactococcus
lactis subsp.
cremoris
Lactobacillus
acidophilus
Bacteriocin
Ph tc dng
c im
Nisin
Lacticin 3147
Lactococcin B
Clostridium sp.
Listeria monocytogenes
Staphylococcus aureus
Streptococcus
dysgalactiae
Enterococcus faecalis
Propionibacterium acne
Streptococcus mutans
Lactobacillus
Acidocin CH5
Lactacin F
Lactacin B
Lactobacillus
amylovorus
Lactobin A
Lactobacillus
casei
Lactocin 705
Leuconostoc
gelidum
Leucocin A
Leuconostoc
mesenteroides
Mesentericin
Y105
Pediococcus
acidilactici
Pediocin F
Bacteriocin lp II, xp x
5 kDa, ph tc dng hp
Bacteriocin lp II, to kt
tp khi lng ln
Lactobacillus fermentum
Bacteriocin lp II, 6,3
Enterococcus faecalis
kDa, 57 acid amin, bn
Lactobacillus delbrueckii
nhit
Lactobacillus helveticus
Lactobacillus delbrueckii
Bacteriocin lp II, 6,3
Lactabacillus helveticus kDa, bn nhit, ch c
Lactobacillus bulgaricus
pht hin khi pH mi
Lactococcus lactis
trng nui cy c gi
n nh t 5,0 6,0
Lactobacillus acidophilus
Bacteriocin lp II, 4,8
Lactobacillus delbrueckii kDa, 50 acid amin, ph
tc dng hp
Listeria monocytogenes Bacteriocin lp II, gm 2
Lactobacillus plantarum
peptid; mi peptid gm
33 acid amin, khi lng
phn t 3,4 kDa
Lactobacillus
Bacteriocin lp II, 3,9
Enterococcus faecalis
kDa, 37 acid amin, bn
Listeria monocytogenes
pH acid, bn nhit
Enterococcus faecalis
Bacteriocin lp II, 3,8
Listeria monocytogenes
kDa, 37 acid amin, bn
nhit
Vi khun Gram (+)
Bacteriocin lp II, 4,5
kDa, bn vi nhit v
Pediocin PA-1
Listeria monocytogenes
Pediocin AcH
Pediococcus
pentosaceous
Pediocin A
Enterococcus
faecium
Enterocin A
Lactobacillus
sake
Lactocin S
Sakacin P
Lactobacillus
curvatus
Lactobacillus
helveticus
Curvacin A
Helveticin J
Lactobacillus
Leuconostoc
Pediococcus
Listeria monocytogenes
Listeria monocytogenes
Enterococcus faecalis
Lactobacillus bulgaricus
Lactococcus lactis
dung mi hu c, hot
ng trong khong pH
rng
Bacteriocin lp II, 4,6
kDa, 44 acid amin
Bacteriocin lp II, 4,6
kDa, 44 acid amin, ph
tc dng rng
Bacteriocin lp II, 2,7
kDa, bn nhit
Nguyn tc
Phng php
spot-on-lawn
(Spot-on-lawn
bn c, do mu th cha
assay) [57]
bacteriocin s c ch s pht
gi vng c ch to thnh.
mu th.
Nh mu th ln cc khoanh giy
Phng php
khuch tn qua
lc v trng, kh ri t ln b
khoanh giy lc
mt mi trng c cy vi sinh
(Disc diffusion
vt ch th. qua m, nh gi
assay) [57]
vng c ch to thnh.
Phng php
Trn mi trng c cy vi
khuch tn qua
ging thch
ging. Nh mu th vo cc
(Well diffusion
assay) [57]
c ch to thnh.
Cho mu th vo ng mi trng
th cha bacteriocin s c ch
(Turbidometric
assay) [41]
S lng t bo c nh gi
c ch ca mu th i vi vi sinh
thng qua c ca mi
vt ch th.
trng.
Tinh ch
Plantaricin ST31
Nisin Z
Enterocin AS48
Plantaricin C19
Pediocin ACM
Salivaricin
Pediocin PA-1
Lactococcin
Mesentericin
Y105
Sakacin A
Sakacin P
Enterocin A
Pediocin A-1
Divercin V41
Acidocin D20079
S
Hiu
bc sut
(%)
3
0,8
1
1
2
2
2
2
5,9
90,0
24,3
15,0
40,4
7,3
3
3
3
29,0
41,0
60,0
3
3
3
3
3
3
10,0
10,0
66,0
25,0
10,0
16,0
64,0
0,07
10,0
2,0
4,4
4
4
1,0
6,4
1,6
7,0
Propionicin F
Pediocin PD-1
0,5
34,0
Chng vi khun
L. acidophilus N
L. acidophilus
11088
Acidophilucin L. acidophilus
A
LAPT 1060
Acidocin
L. acidophilus
8912
TK8912
Acidocin B
L. acidophilus M46
Acidocin A
L. acidophilus
TK9201
Acidocin
L. acidophilus CH5
CH5
Acidocin
L. acidophilus JCM
J1229
1229
Acidocin
L. acidophilus JCM
J1132
1132
Acidophilicin L. acidophilus LA-1
LA-1
Acidocin
L. acidophilus
LF221A
LF221
Acidocin
LF221B
Acidophilin
L. acidophilus IBB
801
801
Acidocin
L. acidophilus
D20079
DSM20079
Acidocin
L. acidophilus
AA11
AA11
Acidocin 1B L. acidophilus
GP1B
Ph tc dng
Cc vi khun thuc h Lactobacillaceae
Cc lactobacilli, Enterococcus faecalis
L. delbrueckii, L. helveticus
Lactobacillus v Lactococcus sp.
L. fermentum, Clostridium sporogenes
Mt s LAB gn gi v Listeria
monocytogenes
Cc chi Lactobacillus, Bacillus, Micrococcus
v Corynebacterium
Lactobacillus v Lactococcus sp.
Lactobacillus sp.
L. delbrucki, L. casei, L. jugurti
Mt s LAB, B. cereus, Clostridium sp., L.
innocua, S. aureus
Mt s lactobacilli gn gi, E. coli v S.
panama
Lactobacillus spp.
Salmonella, Shigella, E. coli, S. aureus, B.
cereus, B. subtilis
Shigella, E. coli, P. aeruginosa, L.
monocytogenes, Yersinia enterocolitica