fB Y T

TRNG I HC DC H NI

PHAN TH AN

NGHIN CU S THAY I
HM LNG V THNH PHN
TINH DU I BI THEO THI GIAN
KHA LUN TT NGHIP DC S

H NI-2013

B Y T
TRNG I HC DC H NI

PHAN TH AN

NGHIN CU S THAY I
HM LNG V THNH PHN
TINH DU I BI THEO THI GIAN
KHA LUN TT NGHIP DC S
Ngi hng dn:
DS. Nghim c Trng
Ni thc hin:
1. B mn Thc vt Trng i hc
Dc H Ni
2. X Yn Ninh huyn Ph Lng tnh
Thi Nguyn

H NI-2013

LI CM N
Kha lun ny c thc hin ti B mn Thc vt Trng i hc
Dc H Ni v x Yn Ninh - huyn Ph Lng tnh Thi Nguyn.
Vi lng knh trng v bit n su sc, ti xin by t li cm n chn
thnh ti DS. Nghim c Trng, ngi thy trc tip hng dn, tn tnh
ch bo, ng vin v to iu kin tt nht cho ti trong qu trnh hon thnh
kha lun tt nghip ny.
Ti cng xin by t li cm n chn thnh ti:
PGS.TS. Trn Vn n, ngi thy hng dn, ch bo tn tnh v
ng gp nhng kin qu bu cho ti trong qu trnh thc hin nghin cu
khoa hc.
Cng cc ging vin B mn Thc vt: TS. Hong Qunh Hoa, TS.
Nguyn Quc Huy, ThS. V Vn Anh, ThS. Phm H Thanh Tng v tt
c cc ch k thut vin B mn Thc vt lun sn sng ch bo v gip ti
hon thnh nghin cu ny.
Anh Trn Vit Vn Cng ty C phn DKNatura, em on Th
Phng Tho A4K65, bn T Khc Cng M1K63 gip ti trong
qu trnh ly mu.
Ti cng xin gi li cm n chn thnh ti cc thy c gio trng i
hc Dc H Ni nhng ngi thy du dt ti sut 5 nm hc va qua.
Cui cng, ti xin chn thnh cm n gia nh v bn b lun bn
cnh, ng vin v gip ti trong qu trnh hon thnh kha lun.
H Ni, ngy 20 thng 5 nm 2013
Sinh vin
Phan Th An

MC LC

Danh mc k hiu, cc ch vit tt

Danh mc cc bng
Danh mc cc hnh

Trang

T VN ...1
PHN 1: TNG QUAN...3
1.1. Loi i bi Blumea balsamifera (L.) DC................................................3
1.1.1. V tr phn loi loi Blumea balsamifera (L.) DC..3
1.1.2. c im thc vt...3
1.1.3. Phn b, thu hi v ch bin...4
1.1.4. Nhng nghin cu v tinh du i bi.....4
1.2. Borneol..7
1.2.1. Cng thc phn t...7
1.2.2. Ngun gc, iu ch....8
1.2.3. Tc dng, cng dng.12
CHNG 2: I TNG V PHNG PHP NGHIN CU..15
2.1. Nguyn vt liu, thit b.15
2.1.1. Nguyn liu nghin cu15
2.1.2. Thit b v ha cht nghin cu16
2.2. Ni dung nghin cu..............................................................................16
2.2.1. Nghin cu c im thc vt.......16
2.2.2. Nghin cu hm lng Borneol16
2.3. Phng php thc nghim17
2.3.1. Nghin cu c im thc vt.......17

2.3.2. Nghin cu hm lng Borneol17

CHNG 3: THC NGHIM, KT QU V BN LUN...19
3.1. Kt qu thc nghim..19
3.1.1. c im thc vt v hm lng Borneol ca cc mu i bi.... 19
3.1.2. S thay i hm lng Mai hoa bng phin theo thi gian..30
3.2. Bn lun...32
KT LUN V KIN NGH.34
Kt lun......34
Kin ngh....35
TI LIU THAM KHO
PH LC
Ph lc 1: Phiu gim nh tn khoa hc
Ph lc 2: Giy chng nhn m s tiu bn
Ph lc 3: Pic sc k GC-MS ca MHBP cc mu nghin cu

DANH MC K HIU, CC CH VIT TT

GABA

Gamma amino butyric acid

GC-MS

Sc k kh khi ph
(Gas chromatography-mass spectrometry)

IC50

Nng c ch 50% i tng th

(Inhibitory Concentration)

MHBP

Mai hoa bng phin

MS

Khi ph (Mass spectrometry)

PTMD

Phn trn mt t

TD

Tinh du

TLTK

Ti liu tham kho

TT

Th t

DANH MC CC BNG

TT

Tn bng

Trang

Bng 1.1: Thnh phn tinh du i bi thu hi ti H

Giang, H Ni, c Lc
2

Bng 1.2: Hm lng v thnh phn tinh du i bi thu

hi ti mt thi im
8

Bng 1.3: Thnh phn tinh du i bi Bangladesh

Bng 1.4: Ngun thc vt cha tinh du c Borneol

9,10,11

Bng 2.1. Danh sch mu i bi

15

Bng 3.1: Cc c im hnh thi khc nhau gia cc

23

mu
6

Bng 3.2: T l Borneol trong Mai hoa bng phin (thng

29

11)
7

Bng 3.3: Hm lng Borneol trong cc mu (thng11)

29

Bng 3.4: Hm lng Mai hoa bng phin trong cc mu

31

nghin cu

DANH MC CC HNH
TT

Tn hnh

Trang

Hnh 1.1: Cng thc cu to ca Borneol

Hnh 1.2: S tng hp Borneol t Camphor

12

Hnh 3.1: c im hnh thi c quan sinh dng

20

16

Hnh 3.2: c im hnh thi c quan sinh sn (1)

21

Hnh3.3: c im hnh thi c quan sinh sn (2)

22

Hnh 3.4: S khc nhau v mu sc thn gn im phn

24

cnh
6

Hnh 3.5: S thay i hnh thi l cc giai on

24

Hnh 3.6: So snh mt lng che ch

24

Hnh 3.7: c im ngn l bc

25

Hnh 3.8: c im mu sc u trng hoa lng tnh

25

10

Hnh 3.9: c im vi phu thn.

26

11

Hnh 3.10: c im vi phu gn l

27

12

Hnh 3.11: c im vi phu phin l

28

13

Hnh 3.12: Biu hm lng Borneol trong dc liu

30

kh tuyt i ca cc mu (thng 11)

14

Hnh 3.13: Biu s thay i hm lng Mai hoa bng

phin 8 mu i bi theo thi gian

32

T VN
Borneol l mt dn cht cha oxy ca monoterpen. Borneol c s
dng trong Y hc c truyn ca nhiu nc trn th gii nh Trung Quc,
Vit Nam, n , Philippines,Trong Y hc c truyn Trung Quc v Vit
nam, Borneol c dng vi tn gi Mai hoa bng phin, c 2 cng nng l:
(i) tiu tn mng mng v (ii) khai khiu tnh thn [9]. Hin nay, Borneol l
mt trong nhng nguyn liu c gi tr cao trong ngnh dc.Theo Dc in
Trung Quc, Borneol l thnh phn quan trng ca khong 63 sn phm t
tho dc [31]. Vit Nam, Borneol c trong thnh phn ca nhiu Dc
phm thuc cc nhm thuc khc nhau nh nhm gim au - khng vim (cao
dn Sinsinpa), nhm thuc tim mch (PC.CARDIO Vin h tm) v cc sn
phm thuc nh mt (Osla, Eyelight Cool, Vimaxx, Optamix,). Hin nay,
trn th trng ha cht c hai loi Borneol khc nhau: (i) Borneol tng hp l
hn hp ca DL-Borneol v Isoborneol (c c tnh) [4]; (ii) Borneol t nhin
c thnh phn chnh l D-Borneol. Theo mt s nghin cu, Borneol tng hp
b bin tnh, phn hy trong qu trnh bo qun v bin i thnh Camphor
(c th ti 45%-97%) l thnh phn c c tnh; trong khi Borneol t nhin
khng c c tnh [16], [30]. V vy, ngy nay ngi ta c xu hng s dng
Borneol t nhin thay v s dng Borneol tng hp.Ngun Borneol thin
nhin ch yu c ly t cc cy thuc h Dipterocarpaceae (Dryobalanops
aromatic C. F. Gaeth), h Asteraceae (Blumea balsamifera (L.)DC.). Hin
nay ngun nguyn liu sn xut Borneol thin nhin ang bi thiu ht, gi ca
Borneol trn th trng tng ln. Do , vic to ngun nguyn liu sn xut
Borneol thin nhin l rt cn thit.
Vit Nam, t thi chng Php, nhn dn ta bit khai thc Borneol
t cy i bi [7].Cy i bi c c tnh qu l d trng, d chm sc, c th

pht trin nhiu ni, trn cc vng t kh hn [24]. T l Borneol v

Camphor trong tinh du i bi thay i theo tng vng: H Giang: 57,82%
Borneol, 1,12% Camphor; H Ni: 50,57% Borneol, 18,71% Camphor; c
Nng: 5,70% Borneol v 70,05% Camphor [5], [11]. Vi cng mt thi im
thu hi, cc mu i bi thu hi ti cc a im khc nhau c hm lng
Borneol trong dc liu kh tuyt i rt thay i: H Ni: 0,03%; Thi
Nguyn: 0,01%; H Giang: 1,75%; Nam nh: 0,26% [8]. khai thc
Borneol t cy i bi t hiu qu cao cn phi nghin cu tm ra thi im
thu hi i bi thch hp, ging i bi v vng trng i bi cho hm lng
Borneol cao.
Vi cc l do trn, bc u nhm to c ngun nguyn liu sn xut
Borneol t nhin t cy i bi v tm ra thi gian thu hi i bi thch hp,
chng ti thc hin ti: Nghin cu s thay i hm lng v thnh
phn tinh du i bi theo thi gian vi cc mc tiu:
1. nh gi s khc nhau v c im thc vt v hm lng Borneol ca
cc mu trong cng mt iu kin sinh thi.
2. Nghin cu s thay i hm lng Mai hoa bng phin ca cc mu
i bi theo thi gian.

CHNG 1: TNG QUAN

1.1. Loi i bi Blumea balsamifera (L.) DC.
1.1.1. V tr phn loi loi Blumea balsamifera (L.) DC.
Chi Blumea DC. c khong 55 loi trn th gii, phn b chu Phi,
ng Nam ti Bc chu i Dng. Vit Nam c 30 loi, gp ph bin t
ng bng, trung du ti min ni [2].
Loi Blumea balsamifera (L.) DC. thuc phn lp cc: Asteridae; Lin b
Cc: Asteranae; B cc: Asterales; H cc: Asteraceae; Chi: Blumea.
Tn ng ngha: Conyza balsamifera L.; Baccharis salviaLour.;Blumea
balsamifera var. microcephala Kitam.; Pluchea balsamifera (L.) Less [2], [3].
1.1.2. c im thc vt
Theo cc ti liu [2], [3], [6], [38] loi Blumea balsamifera (L.) DC. c
cc c im:
Cy bi hoc cy bi thp, cy lu nm, cao 1-3 m. Thn phn cnh
dng ng pha ngn, nhiu lng, gc cnh ha g nhiu, v mu nu xm.
Cnh dng ng thng, dy lng mu trng hi vng. L mc so le, thun di,
kch thc 15-18 x 3,5-5 cm; hp gc l; mt di l ph nhiu lng mt,
lng che ch a bo nm thng so vi mt l; cung l ngn, mang tai l di
10-12 mm; mp l x rng ca tha cho n dy, rng cong ln, nh l nhn;
10-12 cp gn.
Cm hoa u 6-7 mm, c cung, hnh chy; l bc gm 3-4 vng
ngoi, mt ngoi ph nhiu lng p st vo nhau, l bc ngoi nh hn, trn
nhn, di 1-3 mm, l bc trong di hn, hnh di hp di 5-6 mm; ng knh
cm hoa 2,5-3 mm, c l t ong, ph lng; hoa ngoi hnh chy di n 6
mm, x 2-4 thy; hoa gia hnh ng, mu vng, khng cung, di 6-7 mm,
thy c gai tht nh, tha tht lng a bo.

Qu b, mu nu, thun di khong 1 mm; ph lng tha hoc dy; trn

c mo lng hi , di 4-6 mm.
1.1.3. Phn b, thu hi v ch bin
Phn b
i bi mc hoang nhiu Vit Nam, Trung Quc v cc nc nhit i
Nam nh n , Indonesia, Philippines,
Vit Nam, i bi phn b rng, hu ht cc tnh min ni thp di
1000m, trung du, ng bng v nhiu o ln, khng c trong rng su. Cy
d trng, a sng, sng 1-2 nm, c th nhn ging bng ht, cnh hoc cy
con [1], [6], [24].
Thu hi
L i bi c th thu hi 4 t trong 1 nm vi sn lng 50 tn/ha, cho
khong 50-200 kg Borneol [24]. Tinh du l i bi c khai thc t lu i
vi tn thng phm l Mai hoa bng phin. Nhn dn ta s dng Mai hoa
bng phin t th k X. T thi khng chin chng Php, nhn dn ta bit
khai thc Borneol t i bi, sau tm dng. Ma thu ng ct Mai hoa bng
phin cho hm lng Bng phin cao nht. Tinh ch Bng phin bng cch p
bt tinh du, trn Bng phin th vi bt than ci vi t l vi bt: than ci:
Bng phin l 3:5:100; cho hn hp thng hoa, thu ly Bng phin tinh ch
[6].
1.1.4. Nhng nghin cu v tinh du i bi
Nhng nghin cu trong nc
- Trong l i bi thng cha 0,21,8% tinh du vi thnh phn ch
yu l D-Borneol, L-Camphor, Cineol, Limonen, acid Palmitic, acid Myristic,
cn cha cc Sesquiterpen alcol [1], [5].

- Tinh du l i bi c thu thp 3 tnh (H Giang, H Ni, c Lc)

c cc thnh phn chnh c trnh by bng 1.1, vi t l Borneol rt thay
i t 5,70% - 57,82% (Bng1.1) [11].
Bng 1.1: Thnh phn tinh du i bi thu hi ti
H Giang, H Ni, c Lc
a im

H Giang

H Ni

c Lc

Borneol

57,82%

50,57%

5,70%

Camphor

1,12%

18,71%

70,05%

-caryophyllen

8,27%

10,06%

10,54%

-cadinol

7,95%

3,14%

Caryophyllen oxid

3,10%

Patchoulen

2,99%

Veridiflorol

2,01%

Carvacrol

5,70%

Thnh phn chnh

- Vi cng mt thi im thu hi, cc mu i bi thu hi ti cc a

im khc nhau c hm lng tinh du trong dc liu kh tuyt i, hm
lng Borneol v hm lng Camphor trong tinh du rt thay i (Bng 1.2)
[8].

Bng 1.2: Hm lng v thnh phn tinh du i bi

ti mt thi im thu hi
a im
i hc Dc H

HLTD trong DL

HL Borneol trong

HL Camphor

kh tuyt i

tinh du

trong tinh du

0,32%

8,83%

52,38%

0,97%

82,23%

1,89%

0,36%

3,34%

80,63%

2,19%

79,85%

0,72%

1,00%

25,54%

56,43%

Ni, H Ni
Cm Lnh, Ba V,
H Ni
Yn Ninh, Ph
Lng, Thi
Nguyn
V Xuyn, H
Giang
Xun Trng,
Nam nh
Nhng nghin cu trn th gii
L i bi Myanmar cha 1-9% tinh du trong 75% Camphor v 25%
Borneol. Tinh du i bi Philippines c hm lng t 0,1-0,4%. Thi
Lan, trong tinh du, Borneol chim t l 25%, Camphor chim 75%. Tinh du
i bi Trung Quc thnh phn ch yu l Borneol [1].
Tinh du l ti i bi ti Bangladesh c hm lng l 0,40%; xc nh
c 50 thnh phn, trong thnh phn chnh l Borneol (Bng 1.3) [26].

Bng 1.3: Thnh phn tinh du i bi Bangladesh

TT

Thnh phn

T l (%)

-pinene

0,48

-pinene

1,2

D-limonene

0,2

Borneol

33,2

Camphor

0,1

Linalool

1,3

Caryophyllene

8,2

Ledol

7,1

Phytol

4,6

10

Caryophyllene oxide

4,1

11

-eudesmol

3,2

12

Guaiol

3,4

13

Thujopsene

4,4

1.2. Borneol
1.2.1. Cng thc phn t
Borneol l dn cht monoterpen 2 vng cha oxy, cng thc phn t:
C10H18O (Hnh 1.1).

Hnh 1.1: Cng thc cu to ca Borneol

- Tn IUPAC: endo-1,7,7-Trimethyl bicyclo [2.2.1] heptan-2-ol.
- Tn ng ngha: endo-2-bornanol; endo-2-camphanol; endo-2hydroxycamphane; Bornyl alcohol; Borneo camphor; Dryobalanops camphor;
Camphol [21], bng phin, ngi phin, long no hng, i ngi [7].
- Khi lng phn t: 154,25 g/mol. T trng: 1,011 g/cm3 20C. D
thng hoa nhit thng, kh nng thng hoa km hn Camphor; tinh th
khng mu, mi thm c bit v d chu.
- Nhit si: 206C-212C. Nhit nng chy 204C-208C.
- tan: Khng tan trong nc, tan trong alcol: 176:100, ether (1:6);
Benzene (1:5); Toluen; Aceton [21]. Cho phn ng mu vi dung dch
Vanilin sulfuric hay Vanilin clohydric [7].
1.2.2. Ngun gc, iu ch
iu ch t thin nhin
Borneol c th ct cng nghip t: l cy i bi Blumea balsamifera
(L.) DC. (Asteraceae), qu cy Sa nhn Amomum villosum var. xanthioides
(Wall. ex Baker) T.L.Wu & S.J.Chen (Zingiberaceae), cy Long no hng
Dryobalanops aromatic C. F. Gaertn. (Dipterocarpaceae), cy Cinnamomum
burmannii (Ness. & T.Ness) Blume (Lauraceae) [6], [7], [15]. Hn na
Borneol cn l thnh phn trong tinh du ca nhiu cy khc (Bng 3.4).

Bng 1.4: Ngun thc vt cha tinh du c Borneol

TT

Tn khoa hc

Achillea distans Waldst. & Kit. ex

Hm

T l

TL

Tn

phn

lng

Borneol

TK

Vit

khai

TD

(%)

Nam

thc

(%)

Asteraceae

7,5

[13]

Willd.
2

Amomum villosum var. xanthioides

Zingiberaceae

Sa nhn

Ht

2-3

19

[6]

1,5

5,1

[19]

6,24

[25]

30-65

[34]

(Wall. ex Baker) T.L.Wu & S.J.Chen

3

Artemisia afra Jacq. ex Willd

Asteraceae

Blumea martiniana Vaniot

Asteraceae

i bi l
ln, Kim
u

Dipterocarpus turbinatus C. F. Gaernt.

Dipterocarpaceae

Thn

Cinnamomum burmanii (Nees &

Lauraceae

0,60

78,6

[15]

2,4-3,9

2,7

[6]

T.Nees) Blume
7

Kaempferia galanga L.

Zingiberaceae

a lin

10

TT

Tn khoa hc

Micromeria cristata (Hampe) Griseb.

Hm

T l

TL

Tn

phn

lng

Borneol

TK

Vit

khai

TD

(%)

Nam

thc

(%)

Lamiaceae

27-39

[34]

Lamiaceae

1,0-2,3

[17]

Pinaceae

11.52

[22]

Lamiaceae

L,

1,1-6,3

[10]

5,40

[27]

subsp. phrygia P. H. Davis

9

Origanum vulgare subsp. gracile

(K.Koch) Lestw.

10 Pinus koraiensis Siebold & Zucc.

11 Rosmarinus officinalis L.

0,2-1,0

cnh
12 Rosmarinus tournefortii (Noe ex Jord.

Lamiaceae

PTMD

13 Salvia candelabrum Boiss.

Lamiaceae

0,25

4,4

[20]

14 Salvia fruticosa Mill.

Lamiaceae

0,46

1,00

[20]

15 Salvia officinalis subsp. lavandulifolia

Lamiaceae

0,89

2,2

[20]

& Fourr.) Jahand. & Maire

(Vahl) Gams.

11

TT

Tn khoa hc

16 Salvia officinalis L.

Lamiaceae

Hm

T l

TL

Tn

phn

lng

Borneol

TK

Vit

khai

TD

(%)

Nam

thc

(%)

Xn,

1,3-2,6

0,6-7

[10]

0,39

9,3

[20]

Cu tho
17 Salvia tomentosa Mill.

Lamiaceae

17 Satureja kitaibelii Wierzb. ex Heuff

Lamiaceae

PTMD

8,91

[32]

19 Salvia macrochlamys Boiss. & Kotschy

Lamiaceae

13

[33]

20 Thymus algeriensis Boiss. & Reut

Lamiaceae

PTMD

23,48

[16]

12

iu ch t tng hp ha hc
Borneol tng hp bng cch kh ha Camphor (Hnh 1.2) hoc Pinen
(t tinh du thng) cho hn hp ng phn Borneol vi thnh phn ch yu l
Isoborneol [4], [7].

Hnh 1.2: S tng hp Borneol t Camphor

1.2.3. Tc dng, cng dng
Trong y hc c truyn
Bng phin c dng trong ng y t lu i, c xp vo nhm
thuc Phng hng khai khiu.
- Tnh v: v cay, ng, tnh hi hn.
- Quy kinh: tm, t, ph.
- Cng nng ch tr:
Khai khiu tnh thn: dng i vi hu hng sng au, au rng, phi
hp vi phn phi, hong b, ng tm tho.
Tiu tn mng mng: dng khi mt au , mt c mng mng.
- Liu dng: 0,22-0,44g [9].
Ngoi ra, Bng phin cn dng bi ngoi cha mn nht l lot; pha
cn dng xoa bp cha: thp khp, chn thng t mu, st trng ngoi da
[1], [7].

13

Trong y hc hin i
- Tc dng lm tng tnh thm, ci thin sinh kh dng ca thuc dng
phi hp:
Borneol lm tng tnh thm qua gic mc ca thuc thn lipid:
Indomethacin v Dexamethasone l 1,67; 2,00 ln; thuc thn nc:
Ofloxacin, Ribavirin v Tobramycin l 2,15; 2,18 v 3,39 ln. Mt khc, cc
biu m gic mc khng b tn thng v suy yu trc tc ng tng cng
ca Borneol. Borneol l hot cht tim nng c s dng nhm tng s hp
thu ca thuc qua hng ro mu-mt [18].
Borneol lm ko di thi gian gy m ca 2,6-Diisopropylphenol khi s
dng kt hp do Borneol c ch s chuyn ha ca 2,6-Diisopropylphenol
qua gan bng con ng lin hp vi acid Glucuronic [14].
Borneol lm tng s hp thu ca Gastrodin qua ng tiu ha v thc
y phn b n no. So vi vic s dng Gastrodin mt mnh, vic dng kt
hp vi Borneol c th c hp thu nhanh qua ng tiu ha, thi gian t
nng ln nht ca Gastrodin trong huyt tng ngn hn l 5 15 pht so
vi bnh thng l 30 pht, sinh kh dng ca Gastrodigenin trong no tng
33,6%-108,8% [37].
- Tc dng chng vim, st khun
Tinh du i bi c tc dng c ch hot ng ca Bacillus cereus
nng c ch ti thiu (MIC) l 150g/ml, Staphylococus aureaus v
Candida albicans nng c ch ti thiu (MIC) l 120g/ml [35].
- Tc dng gim au, gy m:
Lin quan n tc dng trn cc th th GABA: receptors GABA(A)
kt hp (1) (2) (2L); p ng ca GABA t nht l tng ng vi cc
thuc gy m Etomidate v ln hn nhiu so vi Diazepam hoc 5-pregnan3-ol-20-one [29]. Mt khc, Borneol cn c tc dng c ch receptor

14

Nicotinic acetylcholine (nAChR), c ch nAChR qua ch vn 1,1-dimethy-4phenyl piperazinium iodide vi IC50= 56 9 M. Tc dng c ch nAChR
ca Borneol mnh hn Lidocain, v theo phng thc khng cnh tranh [28].
- Tc dng h huyt p:
Borneol c tc dng h ng k huyt p trn th, lm gim s peroxy
ha lipid, tng hot ng v nng cc enzyme chng oxy ha [23].
- Tc dng chng ng mu:
Nh c tnh ko di cc thng s ng mu nh thi gian Prothrombin
(PT) v thi gian Thrombin (TT) m khng tc ng ln hot ng chng kt
tp tiu cu, Borneol c tc dng chng ng mu; c s dng trong cng
thc phi hp nhm phng nga cc bnh v tim mch [36].
Hin nay, Borneol c dng h tr iu tr cc bnh v phi, dng
bi ti ch kt hp vi cc dng thuc khc nhm iu tr cc chng bnh:
sng hng, l ming, nhim trng tai, bnh vy nn, mn nht, bnh v mt
v thp khp. Dung dch Borneol dng tim, ung tr tim v st khun [4].

15

CHNG 2: I TNG V PHNG PHP

NGHIN CU
2.1. Nguyn vt liu, thit b
2.1.1. Nguyn liu nghin cu
Mu cy Blumea balsamifera (L.) DC. c thu hi ti Yn Ninh Ph
Lng Thi Nguyn. Cc mu cy c ngun gc khc nhau, c ly ging
t cc tnh khc nhau min Bc: H Ni, H Giang, in Bin, Thi
Nguyn. Bt u t thng 3 nm 2011, cc mu c trng ti mt a im
(x Yn Ninh huyn Ph Lng tnh Thi Nguyn) vi cng mt iu
kin chm sc. Khong cch gia cc mu l 2,5 m. Ti thi im bt u
nghin cu, cc mu cao khong 2,5 m. Thi im ly mu trong ngy: 12h13h.
C tng cng 8 mu c thu, x l kh, c gim nh tn khoa
hc v lu tr ti Phng tiu bn B mn Thc vt Trng i hc Dc
H Ni (HNIP) (Bng 2.1).
Bng 2.1. Danh sch mu i bi
TT

S hiu

a im ly ging

A1

Yn Ninh Ph Lng Thi Nguyn

A2

V Xuyn H Giang

A3

ng M Sn Ty H Ni

A4

Qun B - H Giang

A5

Thanh Lung in Bin in Bin

A6

Yn Bi Ba V H Ni

A7

Tng B V Xuyn H Giang

A8

Bc M H Giang

16

- Cc mu c m t c im hnh thi phn trn mt t bao gm:

C quan sinh dng v c quan sinh sn.
- Nghin cu hm lng borneol: l v cnh non cc mu cy i bi
c thu hi vo tun th 4 ca cc thng 9, 10, 11, 12 nm 2012 v cc
thng 1, 2, 3, 4 nm 2013. Nguyn liu c phi kh v bo qun trong ti
nylon.
2.1.2. Thit b v ha cht nghin cu
Thit b:
- Knh hin vi Leica, knh lp soi ni Leica, my nh Canon IXY 30S,
t sy Shellab, kim mi mc, chi lng, phin knh, lamen,...
- B nh lng tinh du nh hn nc ca B mn Thc vt i
hc Dc H Ni.
- B my phn tch GC-MS:
My Agilent Technologies 6890N, detector Agilent Technologies
5973i; ct HP-5MS (30m x 0,25mm x 0,25m).
Ha cht:
- Nc Javen, acid acetic, xanh methylen, son phn, nc ct lm
tiu bn,...
2.2. Ni dung nghin cu
2.2.1. Nghin cu c im thc vt
Nghin cu v c im hnh thi v v cu to gii phu ca 8 mu i
bi. nh gi s khc nhau v c im thc vt ca cc mu trong cng mt
iu kin sinh thi.
2.2.2. Nghin cu hm lng Borneol
Nghin cu s khc nhau v hm lng Borneol ca cc mu i bi
trong cng mt iu kin sinh thi v s thay i hm lng Mai hoa bng
phin trong l i bi theo thi gian.

17

- nh lng hm lng Borneol trong Mai hoa bng phin v trong

dc liu kh tuyt i ca 8 mu i bi thu hi vo thng 11 nm 2012.
- nh lng hm lng Mai hoa bng phin trong dc liu kh tuyt
i ca 8 mu i bi vo cc thng 9, 10, 11, 12 nm 2012 v cc thng 1, 2,
3, 4 nm 2013.
2.3. Phng php thc nghim
2.3.1. Nghin cu c im thc vt
- Nghin cu v c im hnh thi c quan st bng mt thng,
knh lp soi ni v m t theo phng php m t phn tch.
- Nghin cu v cu to gii phu: s dng phng php nhum kp,
quan st trn knh hin vi, m t theo phng php m t phn tch.
2.3.2. Nghin cu hm lng Borneol
nh lng Mai hoa bng phin trong i bi
Nguyn liu c ct nh, ct tinh du bng b nh lng tinh du nh
hn nc, theo phng php ct ko hi nc, cho n khi khng cn tinh
du trong dc liu, Mai hoa bng phin thu c di dng rn, em cn
xc dnh hm lng MHBP.
Song song tin hnh xc nh hm m dc liu theo phng php
nhit , s dng my o hm m nhit l 100C.
Hm lng Mai hoa bng phin trong dc liu kh tuyt i c xc
nh theo cng thc:

Trong : a: khi lng Mai hoa bng phin (g)

H: hm m dc liu (%)
m: khi lng nguyn liu (g)

18

X: hm lng Mai hoa bng phin (%)

Xc nh hm lng Borneol trong dc liu
Phn tch thnh phn tinh du bng phng php sc k kh khi ph
(GC-MS) ti Vin Php y Qun i. iu kin phn tch: Tc dng kh He
0,9 ml/pht, nhit bung bm mu 265C, nhit detector 280C, nhit
MS source 230C, nhit quard 150C. Chng trnh nhit ca ct l:
70C gi 2 pht, tng 30C/pht n 190C, tng tip 5C/pht n 270C,
gi 15 pht. Bm mu theo ch chia dng 10:1, th tch bm mu 1l.
Hm lng Borneol trong dc liu c tnh theo cng thc:
Y= X.A
Trong :
Y: Hm lng Borneol trong dc liu (%)
X: Hm lng MHBP trong dc liu (%)
A: T l Borneol trong MHBP (%)

19

CHNG 3: THC NGHIM, KT QU V BN LUN

3.1. Kt qu thc nghim
3.1.1. c im thc vt v hm lngBorneol ca cc mu i bi
c im hnh thi
Cc mu i bi thu c c c im hnh thi tng i ging nhau,
cc c im chung ca cc mu c m t nh sau:
Cy bi, cao 1-3m, mu xanh phn thn non, nu xanh phn thn gi.
Thn tit din gn trn, c kha dc, ng knh 5-10 mm. Ton cy c mi
thm v c lng t mn. Phn cnh dng ng pha ngn. L n, mc cch.
Phin l thun di, kch thc 8-27 x 2,0-7,6 cm, mt trn mu xanh m, t
lng hn mt di. Mt di l ph nhiu lng mt. Mp l x rng ca.
Gn l hnh lng chim ni r mt di, c 9-17 cp gn.Cung l hnh tr,
mu xanh, hi phng mt trn; mang 1-4 cp ph b kch thc nh dn v
pha y cung (Hnh 3.1). Cm hoa u thng hp thnh chm xim ngn
cnh hay nch l, kch thc rt thay i. u hnh trng khi l n, khi hoa n
u c hnh chung hi tht gia, kch thc khong 0,61,0 x 0,30,5 cm;
cung ngn 0,10,6 cm, c 1-3 vy ngn. Tng bao l bc 57 hng l bc
xp kt lp, hnh bu dc n di hp, vng trong di hn vng ngoi, kch
thc 15 x 0,20,5 mm, mu xanh, nh mu nu hoc nu tm, ra mng,
mt ngoi c nhiu lng. cm hoa hi li, ng knh khong 2 4 mm,
l t ong. u mang hoa hnh ng, c hai loi hoa: hoa ci rt nhiu xp 3-4
vng ngoi, hoa lng tnh 18-25 hoa trong (Hnh 3.2). Hoa ci mu vng
nht, nhng hoa ngoi cng thng c hnh tr cong; i bin i thnh tm
lng di mu trng; trng hoa mu vng nht, dnh thnh mt ng hp, di 46mm, trn chia 2-3 thy tam gic nh, c lng tit tinh du thm; bu di 1

20

, mu trng trong m, hnh tr hp gc; vi nhy vng nht, dng si di

khong 57 mm, tn cng l 2 nhnh u nhy mu vng m hnh mng hp
di 12 mm. Hoa lng tnh ln hn hoa ci, mu vng nht; trng hoa hnh
ng di, loe dn, trn chia 5 thy hnh tam gic c mu vng m; nh 5, ch
nh ri, dng si, di khong 12 mm, mu vng nht, bao phn dnh nhau
thnh ng di khong 23 mm, bao ly vi nhy, mu vng m; vi nhy
dng si di khong 57 mm, u nhy 2, di khong 12 mm, mu vng
m, dng mng cn, thun hp. Qu b mu vng nu, hnh tr di khong
0,51mm, mt ngoi v c lng ngn mu trng v c 4-6 g dc, trn mang
tm lng mo di, mu trng ca i tn ti, gc c vng g ch gn vi
hoa; 1 ht (Hnh 3.3).

1: Cnh, 2: Chiu di l, 3: Chiu rng l

Hnh 3.1: c im hnh thi c quan sinh dng

21

1: Cm hoa; 2: L bc; 3: Hoa ci v hoa lng tnh; 4: cm hoa ct dc

Hnh 3.2: c im hnh thi c quan sinh sn (1)

22

1: u ng trng hoa lng tnh, 2: Ch nh v bao phn,

3: Bao phn ct dc, 4: Qu mang mo lng
Hnh3.3: c im hnh thi c quan sinh sn (2)

23

Hnh thi cc mu i bi c mt s c im khc nhau (Bng 3.1):

Bng 3.1: Cc c im hnh thi khc nhau gia cc mu
c im

M t

A1 A2 A3 A4 A5 A6 A7 A8

Mu sc thn gn im
phn cnh (Hnh 3.4)

Xanh

Mt lng che ch
(Hnh 3.6)
Hnh thi l qua cc giai
on (Hnh 3.5)
Mu sc nh thy ca
hoa lng tnh (Hnh 3.8)

c im ngn l bc
(Hnh 3.7)

Hi
tm
t
Nhiu

Mu
nu
tm
T l trung bnh chiu di l/
chiu rng l

x
x
x

Thay
i
Khng x
i
Vng x
Hi
tm
Mu
nu

x
x

x
x

x
x

x
x

x
x

x
x

x
x

x
x

5,4 3,7 6,2 4,4 6,7 3,6 3,6 5,0

24

1: Thn mu xanh, 2: Thn mu tm

Hnh 3.4: S khc nhau v mu sc

Hnh 3.5: S thay i hnh

thi l cc giai on

thn gn im phn cnh

1: Lng che ch t v ngn, 2: Lng che ch nhiu v di

Hnh 3.6: So snh mt lng che ch

25

Mu nu tm

Mu nu

Hnh 3.7: c im ngn l bc

Mu tm hng

Mu vng

Hnh 3.8: c im mu sc u trng hoa lng tnh

26

c im vi phu
c im vi phu ca cc mu khng c s khc bit, c m t nh sau:
- Vi phu thn: Hnh 3.9.
2

1 6 7

10
8

11

10

1: Biu b; 2: Lng che ch; 3: Lng tit a bo; 4: M dy; 5: T bo tit

tinh du; 6: M cng; 7: Libe; 8: Tng pht sinh libe - g; 9: G; 10: M mm
rut.
Hnh 3.9: c im vi phu thn
Mt ct ca thn c hnh gn trn, t ngoi vo trong bao gm:
(1) Lp biu b: l mt lp t bo nh xp ngoi cng, hnh ch nht
hoc hnh trn, khng u. Trn lp biu b c nhiu lng che ch a bo v
lng tit a bo. (2) Lng che ch a bo c cu to gm mt dy 3 5 t bo
di pha trn v nhiu t bo nh, ngn xp trn 1 3 hng gn gc. (3)
Lng tit a bo c chn di hoc ngn, u hnh trng thun cu to bi
nhiu hng t bo c lp cutin ph thnh m ch. (4) M dy l 4 8 lp t
bo trn hoc a gic gn trn, bt mu hng m, kch thc khng u
nhau, ln hn hoc bng t bo biu b. (5) T bo tit tinh du ri rc trong

27

m dy, m mm v, m mm rut. Cc b libe-g xp theo mt vng trn.

(6) M cng xp thnh tng cm khng u trn libe. (7) Libe nhiu t bo
hnh a gic, kch thc nh, bt mu hng. (8) Tng pht sinh libe-g gia
libe v g, gm nhiu lp t bo nh, c vch t bo mng. (9) G c mch
g hnh trn, m mm g gm nhiu t bo hnh a gic vch cellulose. (10)
M mm rut chim mt vng rng, gm nhiu t bo hnh trn hoc gn
trn, kch thc khng u.
- Vi phu gn l: Hnh 3.10.

6
6

5
2
4

7
1 3

1: Biu b; 2: Lng tit a bo; 3: Lng che ch; 4: M dy; 5: Libe; 6: G; 7:

T bo tit; 8: M mm
Hnh 3.10: c im vi phu gn l
Gn l mt trn li cao hi bng nh, mt di phnh trn tht hai
bn phin l, i khi c g li ph. (1) T bo biu b hnh bu dc hay ch
nht kch thc khng u, c rt nhiu lng che ch v lng tit a bo. (2)
Lng tit bo v (3) lng che ch c cu trc ging thn. (4) M dy: 5 7

28

lp t bo di biu b trn v 1-6 lp t bo trn biu b di, t bo kch

thc khng u. (5) Libe: t bo kch thc nh, hnh a gic, xp ln xn,
bt mu hng. (6) Mch g hnh trn, xp thnh 3-8 dy xen k vi 1-2 dy t
bo m mm g vch cellulose. (7) T bo tit ri rc trong m mm. (8) M
mm: gm nhiu lp t bo hnh trn, thnh mng.
- Vi phu phin l: Hnh 3.11.

1: Biu b trn; 2: Biu b di; 3 M du; 4: Lng che ch;

5: Lng tit; 6: T bo tit
Hnh 3.11: c im vi phu phin l
(1) Biu b trn t bo hnh bu dc, kch thc khng u. (2) Biu b
di t bo c kch thc nh hn biu b trn nhiu ln. C 2 lp biu b u
c nhiu lng che ch v lng tit a bo c cu to ging nh thn. (3) M
giu n su vo phn gn gia, gm 1 lp t bo. T bo m giu hnh ch
nht c chiu di gp 3 ln chiu rng.T bo tit nm ri rc trn phin l.
Hm lng Borneol
Cc mu Mai hoa bng phin ca 8 mu i bi thu hi vo thng 11
nm 2012 c phn tch cho thy: tt c cc mu u cha Borneol v
Camphor. Pic sc k ca cc mu MHBP c trnh by Ph lc 3.

29

T l Borneol trong cc mu Mai hoa bng phin thay i t 95,6% 99,7% (Bng 3.2).
Bng 3.2: T l Borneol trong Mai hoa bng phin (thng 11)
Mu

T l Borneol (%)

A1

99,5

A2

99,3

A3

95,6

A4

99,2

A5

98,3

A6

98,3

A7

99,7

A8

99,5

Hm lng Borneol trong dc liu kh tuyt i ca cc mu thay i

t 0,63%-0,84% (Bng 3.3).
Bng 3.3: Hm lng Borneol trong cc mu (thng 11)
Mu

Hm lng Borneol (%)

A1

0,84

A2

0,67

A3

0,73

A4

0,64

A5

0,73

A6

0,68

A7

0,68

A8

0,63

30

Hm lng Borneol trong dc liu kh tuyt i ca cc mu c s

chnh lch khng nhiu (Hnh 3.12).

%Borneol
,900%

,800%

,700%

,600%

,500%
%Borneol
,400%

,300%

,200%

,100%

,000%
A1

A2

A3

A4

A5

A6

A7

A8

Mu

Hnh 3.12: Biu hm lng Borneol trong dc liu

kh tuyt i ca cc mu (thng 11)
3.1.2. S thay i hm lng Mai hoa bng phin theo thi gian
Kt qu nh lng hm lng Mai hoa bng phin trong dc liu kh
tuyt i ca cc mu i bi vo cc thng 9, 10, 11, 12 nm 2012 v cc
thng 1, 2, 3, 4 nm 2013 nh sau (Bng 3.4):

31

Bng 3.4: Hm lng Mai hoa bng phin trong cc mu nghin cu

Nm 2012
Mu

Thng

Nm 2013

Thng

Thng

Thng

Thng

Thng

Thng

Thng

9 (%)

10 (%) 11 (%) 12 (%)

1 (%)

2 (%)

3 (%)

4 (%)

A1

1,54

1,51

0,84

0,78

0,52

0,44

0,42

0,61

A2

1,40

1,41

0,67

0,39

0,29

0,26

0,23

0,36

A3

1,37

1,36

0,76

0,74

0,26

0,24

0,23

0,33

A4

1,31

0,72

0,65

0,39

0,31

0,25

0,22

0,39

A5

1,15

1,02

0,74

0,45

0,29

0,28

0,23

0,29

A6

1,12

1,00

0,69

0,68

0,32

0,25

0,24

0,34

A7

1,08

0,94

0,68

0,38

0,28

0,24

0,22

0,38

A8

1,00

0,89

0,63

0,49

0,30

0,28

0,26

0,35

Hm lng Mai hoa bng phin trong dc liu kh tuyt i ca cc

mu i bi nm trong khong 0,22% - 1,54%. Cc mu i bi c hm lng
MHBP cao nht vo thng 9/2012, gim dn v thp nht vo thng 3/2013 l
thi im cy ra hoa, sau li tng ln vo thng 4/2013 l thi im cy
u qu (Hnh 3.13).

32

1,800%

%Borneol
A1
A2

1,600%

A3
1,400%

A4
A5

1,200%

A6
1,000%

A7
A8

,800%

,600%

,400%

,200%

,000%
T9

T10

T11

T12

T1

T2

T3

T4 Thi gian

Hnh 3.13: Biu s thay i hm lng Mai hoa bng phin

8 mu i bi theo thi gian

3.2. Bn lun
Theo mt nghin cu v tnh a dng sinh hc cy i bi min Bc
Vit Nam, cc cy thuc loi Blumea balsamifera (L.) DC. kh a dng. Vi
cng mt thi im thu hi, cc mu i bi thu hi cc a im khc nhau
c s khc bit ln v hm lng tinh du (0,32%-2,19%) v hm lng
Borneol trong tinh du (3,34%-82,23%) [10]. c nh cc bin v iu
kin kh hu, th nhng v chm sc, cc mu i bi c ly ging t cc
tnh khc nhau: H Giang, H Ni, in Bin, Thi Nguyn; trng ti mt a
im (Yn Ninh Ph Lng Thi Nguyn).

33

Kt qu nghin cu v hnh thi cho thy cc mu i bi c s khc

nhau v mu sc thn, mu sc nh thy hoa lng tnh, c im ngn l
bc, mt lng che ch. Kt qu nh lng hm lng v thnh phn Mai
hoa bng phin ca cc mu i bi ti cng mt thi im thu hi (thng 11
nm 2012) khng c s khc bit r rt: Hm lng Mai hoa bng phin t
0,63%-0,84%; hm lng Borneol trong MHBP t 95,6%-99,7%; hm lng
Borneol trong dc liu kh tuyt i ca cc mu t 0,63%-0,84%. Nh vy,
sau khi c nh cc bin thuc iu kin sinh thi th s sai khc v hm
lng MHBP v Borneol gia cc mu gim i ng k, cc mu c xu
hng ging nhau v hm lng v thnh phn MHBP. Kt qu ny bc u
ch ra rng iu kin sinh thi c nh hng nhiu n hm lng v thnh
phn tinh du i bi. Tuy nhin, ti thi im hm lng MHBP ln nht
(thng 9), hm lng MHBP trong cc mu i bi c s khc bit r rt hn
(1,00%-1,54%). iu ny cho thy yu t ngun gen c nh hng n hm
lng MHBP ca cc mu i bi.
Kt qu nh lng hm lng Mai hoa bng phin cho thy hm lng
MHBP ca cc mu i bi cao nht vo thng 9 nm 2012 (1,00% - 1,54%),
gim dn v thp nht vo thng 3 nm 2013 (0,22 0,42%), sau li tng
ln vo thng 4 nm 2013 (0,29%-0,61%). Thi im hm lng MHBP thp
nht l ma xun, lc cy ra hoa v thi im hm lng MHBP cao nht l
ma thu, iu ny ph hp vi kinh nghim thu hi i bi ca nhn dn ta v
mt nghin cu trc ti Vit Nam [8]. Tuy nhin, cn phi tip tc nh
lng hm lng Mai hoa bng phin vo cc thng 5, 6, 7, 8 ca nm 2013
v thnh phn MHBP ca tt c cc thng trong nm tm ra thi im thu
hi i bi ti u nht.
Kt qu phn tch thnh phn tinh du cho thy hm lng Borneol
trong Mai hoa bng phin ca cc mu t 95,6% - 99,7% l rt cao, trong khi

34

hm lng ca Borneol trong tinh du ca mt s cy c s dng ct

trong

cng

nghip

nh

Dipterocarpus

turbinatus

C.

F.

Gaertn.

(Dipterocarpaceae) 30%-65% [40], Amomum villosum var. xanthioides (Wall.

ex Baker) T.L.Wu & S.J.Chen (Zingiberaceae) 19% [8], Micromeria cristata
(Hampe) Griseb.subsp. phrygia P. H. Davis (Lamiaceae) 27% - 39% [40].
Chng t y l ngun nguyn liu ph hp dng khai thc Borneol thin
nhin. Hm lng Borneol trong Mai hoa bng phin ca tt c cc mu
nghin cu ln hn nhiu so vi hm lng Borneol trong tinh du ca cc
mu i bi trong cc nghin cu trc ti Vit Nam (3,34% - 82,23%)
[14], [10], [7]. Do cn tip tc nghin cu cc c im sinh thi c lin
quan n hm lng Borneol to c vng trng nguyn liu khai thc
Borneol t cy i bi.

KT LUN V KIN NGH

Kt lun:
Nghin cu m t chi tit c c im hnh thi, vi phu ca 8 mu
i bi c ngun gc khc nhau t H Ni, H Giang, Thi Nguyn, in Bin
c trng ti cng mt a im (Yn Ninh - Ph Lng - Thi Nguyn) c
s khc nhau v hnh thi: mu sc thn, mu sc nh trng hoa lng tnh,
mu sc nh l bc, mt lng che ch, Vi cng mt thi im thu hi
l thng 11/2012, 8 mu i bi khng c s khc bit r rt v hm lng
Borneol trong dc liu kh tuyt i (0,63%-0,84%). Hm lng Borneol
trong MHBP ca cc mu xp x bng nhau v rt cao (95,6%-99,7%).
Nghin cu s thay i hm lng Mai hoa bng phin trong cc mu
i bi theo thi gian t thng 9/2012 n thng 4/2013: hm lng MHBP
trong dc liu kh tuyt i cao nht vo thng 9/2012 (1,00% - 1,54%),
gim dn v thp nht vo thng 3/2013 (0,22% 0,42%) l thi im cy ra

35

hoa, sau li tng ln vo thng 4/2013 (0,29%-0,61%) l thi im cy u

qu.
Kin ngh:
Trn y l cc kt qu bc u m chng ti t c trong iu kin
thi gian v kh nng cho php. tip tc thc hin ti, chng ti kin
ngh:
-

Nghin cu s thay i hm lng Borneol trong MHBP theo thi

gian v nh lng hm lng MHBP trong cc mu vo cc thng 5, 6, 7, 8

ca nm 2013.
-

Theo di , kim tra s thay i v hnh thi, hm lng Borneol ca

cc mu i bi c cng ngun gc trong cc iu kin sinh thi khc nhau.

-

Nghin cu s thay i hm lng Borneol trong l i bi theo s

pht trin ca l: L non, l bnh t v l gi.

TI LIU THAM KHO

Ti liu ting Vit
1. Huy Bch, ng Quang Trung v cng s (2004), Nhng cy thuc v
ng vt lm thuc Vit Nam, NXB Khoa hc v K thut, trang 722-724.
2. L Kim Bin (2007), Thc vt ch Vit Nam, NXB Khoa hc v K thut,
tp 7, tr. 218-219.
3. V Vn Chi (1997), T in cy thuc Vit Nam, NXB Y hc, trang 1461.
4. Nguyn Duy Cng, Nguyn Hu Qunh v cng s (1999), T in bch
khoa dc hc, NXB T in bch khoa H Ni, tr. 80.
5. Phm Thanh K (2007), Dc liu hc tp 2, NXB Y hc, tr. 174-187, tr.
240-241.
6. Tt Li (1999), Nhng cy thuc v v thuc Vit Nam, NXB Y hc, tr.
605-607, tr. 402-403.
7. Tt Li (1985), Tinh Du Vit Nam, NXB Y hc, tr 118-120.
8. Phm Th Thy Oanh (2012), iu tra tnh a dng sinh hc cy i bi
min Bc Vit Nam, Trung tm thng tin Th vin H Dc H Ni.
9. Phm Xun Sinh (2006), Dc hc c truyn, Nh xut bn Y hc, tr. 212215.
10. Nguyn Th Tn (2003), Nhng tinh du lu hnh trn th trng, NXB
Khoa hc v K thut H Ni, tr. 125-127.
11. Nguyn Th Tm, L Nguyn L Thu, Nguyn Vit Thn, Nguyn Quang
Thy (2004), Nghin cu thnh phn tinh du l i bi Blumea balsamifera
(L.) DC. Vit Nam, Tp ch dc hc, S (6), tr. 12-13.
Ti liu ting Anh
12. Ait-Ouazzou A (2011), Chemical composition and antimicrobial activity
of essential oils of Thymus algeriensis, Eucalyptus globulus and Rosmarinus

officinalis from Morocco, Journal of the Science of Food Agriculture, 91

(14), p.2643-2651.
13. Konakchiev A (2011), Composition and antimicrobial activity of
Achillea distans essential oils, Natural Product Communication, 6(6), p.905906.
14. Li Lin A, Shangari N, Chan TS, Remirez D, OBrien PJ (2006), Herbal
momoterpene alcohols inhibit propofol metabolism and prolong anesthesia
time, Life Science, 79(1), p.21-29.
15. Lei Chen, Jianyu Su, Lin Li, Bing Li, Wang Li (2011), A new source of
natural D-borneol and its characteristic, Journal of Medicinal Plants
Research, 5(15), p.3440-3447.
16. Zeng CQ , He GF (2004), Analysis of ten batches of borneol on market
by GC-MS, The Journal Chiness of Medicine, (27), p.347.
17.Sharopov FS, Kukaniev MA, Setzer WN (2001), Composition of the
essential oil of Origanum tyttanthum from Tajikistan, Natural Product
Communication, 6(11), p.1719-1722.
18. Yang H, Xun Y, Li Z, Hang T, Zhang X, Cui H (2009), Influence of
borneol on in vitro corneal permeability and on in vivo and in vitro corneal
toxicity, The Journal of International Medical Reseach, 6(3), p.791-802.
19. Mwang I (1995), Essential oil constituents of Artemisia afra
Willd.,Journal of Essential Oil Research, 7(1), p.97-99.
20. Masthes, Imre, Masthes, Askos, Hohmann Judit, Janicsask, Gasbor
(1999), Evaluation of the essential oil production and composition of Salvia
species beyond their native area of distribution, Journal of Medicinal Plants
Research, 52(2), p.263-208.
21. Maryadele J. ONeil (2006), The Merck Index.

22. Kim JH, Lee HJ, Jeong SJ, Lee MH, Kim SH (2012), Essential Oil of
Pinus koraiensis Leaves Exerts Antihyperlipidemic Effects via Up-regulation
of Low-density Lipoprotein Receptor and Inhibition of Acyl-coenzym A:
Cholesterol Acyltransferase, Phytotherapy Research, (10), p.1002.
23. Murugesan Saravana Kumar, Subramaniyan Kumar, Boobalan Raja
(2010), Antihypertensive and Antioxidant Potential of Borneol-A Natural
Terpene in L NAME Induced Hypertensive Rats, International Journal
of Pharmaceutical & Biological Archives, 1(3), p.271-279.
24. A.P.L, Oyen, Nguyen Xuan Dung (1999), Plant Resources of South-East
Esia 19, Essential-oil Plants, Bachuys Publisher, Leiden, p.68-70.
25. Zhu L, Tian YJ. (Chemical composition and larvicidal effects of essential
oil of Blumea martiniana against Anopheles anthropophagus, Asian Pacific
Journal of Tropical Medicine, 4(5), p.371-374.
26. Md. Nazrul Islam bhuiyan, Jasim Uddin Chowdhury, Jaripa Begum
(2009), Chemical components in volatile oil from Blumea balsamifera (L.)
DC.,Bangladesh Journal of Botany, 38(1), p.107-109.
27. Bendeddouche MS, Benhassaini H, Hazem Z, Romane A (2011),
Essential oil analysis and antibacterial activity of Rosmarinus tournefortii
from Algeria, Natural Product Communication, 6(10), p.1511-1514.
28. Tea Ju Park (2003), Inhibition of acetylcholine-mediated effects by
Borneol, Biochemical Pharmacology, (65), p.83-90.
29. Granger RE Campbell EL, Johnston GA (2005), (+)-And (-)-borneol:
efficacious positive modulators of GABA action at human recombinant
[alpha]1[beta]2[gamma] 2L GABAA receptors, Biochemical pharmacology,
69(7), p.1101-1111.

30. Manoguerra S., Pharm.D., Andrew R. Erdman (2006), Camphor

Poisoning: An Evidence-Based Practice Guideline for Out-of-Hospital
Management, Clinical Toxicologi, (44), p.357-370.
31. State Pharmacopoeia Committee (2005), The Pharmacopoeia of Peoples
Republic of China, First edition, Chemical Industry Press, p.98-99.
Mihajilov-Krstev T (2011), Chemical composition and antimicrobial activity
of Satureja kitaibelii essential oil against pathogenic microbial strains,
Natural Product Communication, 6(8), p.1167-1172.
32. Tabanca T, Demirci B, Kirimer N, Husnu CBK, Zeki A, Murat Ekici
(2006), Chemical Composition and Antifungal Activity of Salvia
macrochlamys and Salvia recognita Essential Oils, Jounral of Agricultura of
Food Chemistry, 54(18), p.6593-6597.
33. Tabanca T, Kirimer N, Demirci Betl, Demirci F, Hsn K, Baser C
(2001), Composition and antimicrobial activity of the essential oils of
Micromeria cristata subsp. phrygia and the enantiomeric distribution of
borneol, Jounral of Agricultural of Food Chemistry, 49(9), p.4300-4303.
34. Cushnie TP, De-Eknamkul W, Maneerat S, Sakee U (2011),
Antimicrobial activity of Blumea balsamifera (L.) DC. extracts and essential
oil, Natura Product Research, 25(19), p.49-56.
35. Li YH, Sun XP, Zhang YQ, Wang NS (2008), The antithrombotic effect
of borneol related to its anticoagulant property, The American Journal of
Chinesse Medicine, 36(4), p. 719-727.
36. Cai Z, Hou S (2008), Effect of borneol on the distribution of gastrodin to
the brain in mice via oral administration, Journal of Drug Targeting, 16(2),
p. 178-184.
Ti liu trn Internet
http://www.efloras.org/florataxon.aspx?flora_id=3&taxon_id=200023545

PH LC 1
TRNG I HC DC H NI
B MN THC VT
*****
PHIU GIM NH TN KHOA HC
S: 02/2013
Ngi thu mu:

Phan Th An

Ngi gi mu:

Phan Th An A3K63 H Dc H Ni

Ngy thu mu:

(Bng km theo)

Ni thu mu:

(Bng km theo)

Tn a phng:
Yu cu:

Gim nh tn khoa hc

M t mu:

8 mu, gm thn mang l, hoa

Kt qu gim nh: Cn c vo cc ti liu thc vt hin c ti Trng H Dc

H Ni (bao gm: Thc vt ch Vit Nam H Asteraceae; Cy c Vit Nam Tp
3; T in Thc vt thng dng Tp 1; Flora of China, Vol. 20-21;Flora of
Taiwan Compositae;Flora Reipublicae Popularis Sinicae Tomus 75; v cc
mu lu ti phng tiu bn National Taiwan University TAI; Herbier Musum
Paris Musum National dHistoire Naturelle vi cc c im ca cc b phn
mu cy, xc nh mu trn c:
-

Tn khoa hc:

Blumea balsamifera (L.) DC.

H:

Asteraceae

Tn thng gi:

i Bi
H Ni, ngy 16 thng 05 nm 2013
B MN THC VT
Ngi gim nh

DS. Nghim c Trng

Bng danh sch cc cy gi gim nh

TT

Ni thu mu

Ngy thu mu

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

Yn Ninh Ph Lng
Thi Nguyn

30/03/2013

K hiu
mu

A1
A2
A3
A4
A5
A6
A7
A8

Ngi thu mu

Phan Th An
Phan Th An
Phan Th An
Phan Th An
Phan Th An
Phan Th An
Phan Th An
Phan Th An

TRNG I HC DC H NI

B MN THC VT

PHNG TIU BN CY THUC (HNIP)

***

GIY CHNG NHN M S TIU BN

1. Tn mu cy
- Tn khoa hc: (bng km theo)
- Tn thng dng: (bng km theo)
- Tn a phng: (bng km theo)
2. Ngun gc: Yn Ninh Ph Lng Thi Nguyn
3. Ngy thu mu: (bng km theo)
4. Ngy np mu: 16/05/2013
5. Ngi thu mu: Phan Th An

C quan: A3K63, H Dc HN

6. Ngi np mu: Phan Th An

C quan: A3K63, H Dc HN

7. S hiu phng tiu bn: (bng km theo)

8. Ngi gim nh tn khoa hc: DS. Nghim c Trng
9. S lng mu np: 23

Ngi np mu

Phan Th An

Ngi nhn mu

TS. Nguyn Quc Huy

T
T

MS

A1

A2

A3

A4

A5

A6

A7

A8

Tn khoa hc
Blumea
balsamifera (L.)
DC.
Blumea
balsamifera (L.)
DC.
Blumea
balsamifera (L.)
DC.
Blumea
balsamifera (L.)
DC.
Blumea
balsamifera (L.)
DC.
Blumea
balsamifera (L.)
DC.
Blumea
balsamifera (L.)
DC.
Blumea
balsamifera (L.)
DC.

Tn
thng
gi

Asteraceae i bi
Asteraceae i bi
Asteraceae i bi
Asteraceae i bi
Asteraceae i bi
Asteraceae i bi
Asteraceae i bi
Asteraceae i bi

Tn a
phng

a im ly
ging

MSTB

S
TB

i ngi

Ph LngThi Nguyn

HNIP/18041
/13

i ngi

V Xuyn-H
Giang

HNIP/18042
/13

i ngi

Sn Ty-H
Ni

HNIP/18043
/13

Qun B-H
Giang
Thanh
Lung-in
Bin

HNIP/18044
/13

HNIP/18045
/13

HNIP/18046
/13

HNIP/18047
/13

HNIP/18048
/13

i ngi
i ngi

i ngi

Ba V-H
Ni
Tng B-V
Xuyn-H
Giang

i ngi

Bc M-H
Giang

i ngi

PH LC 3
PIC SC K GC-MS CC MU MAI HOA BNG PHIN

A b u n d a n c e
T IC : 9 5 B O R N E O L A 1 L A N 2 .D \ d a ta .m s

1 .2 e + 0 7
1 5 .0 5 7
1 .1 e + 0 7

1 e + 0 7

9 0 0 0 0 0 0

8 0 0 0 0 0 0

7 0 0 0 0 0 0

6 0 0 0 0 0 0

5 0 0 0 0 0 0

4 0 0 0 0 0 0

3 0 0 0 0 0 0

2 0 0 0 0 0 0

1 0 0 0 0 0 0
1 4 .4 3 7
1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A1

2 8 .0 0

A b u n d a n c e
T IC : 9 5 B O R N E O L A 2 .D \ d a ta .m s
1 .1 e + 0 7
1 5 .0 4 6

1 e + 0 7

9 0 0 0 0 0 0

8 0 0 0 0 0 0

7 0 0 0 0 0 0

6 0 0 0 0 0 0

5 0 0 0 0 0 0

4 0 0 0 0 0 0

3 0 0 0 0 0 0

2 0 0 0 0 0 0

1 0 0 0 0 0 0
1 4 .4 3 0
1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

2 8 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A2

Abundance
T IC : 9 5 B O R N E O L A 3 .D \ d a ta .m s
7000000
6500000

1 5 .0 2 1

6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000

1 4 .4 1 6
1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A3

2 8 .0 0

A b u n d a n c e

T IC : 9 5 B O R N E O L A 4 .D \ d a ta .m s
1 5 .0 6 5

1 .2 e + 0 7

1 .1 e + 0 7

1 e + 0 7

9 0 0 0 0 0 0

8 0 0 0 0 0 0

7 0 0 0 0 0 0

6 0 0 0 0 0 0

5 0 0 0 0 0 0

4 0 0 0 0 0 0

3 0 0 0 0 0 0

2 0 0 0 0 0 0

1 0 0 0 0 0 0
1 4 .4 3 0
8 .0 0

1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

2 8 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A4

A b u n d a n c e
T IC : 9 5 B O R N E O L A 5 .D \ d a ta .m s

9 0 0 0 0 0 0

1 5 .0 3 3
8 0 0 0 0 0 0

7 0 0 0 0 0 0

6 0 0 0 0 0 0

5 0 0 0 0 0 0

4 0 0 0 0 0 0

3 0 0 0 0 0 0

2 0 0 0 0 0 0

1 0 0 0 0 0 0
1 4 .4 2 5
1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A5

2 8 .0 0

A b u n d a n c e

T IC : 9 5 B O R N E O L A 6 .D \ d a ta .m s
1 5 .0 4 0

9 0 0 0 0 0 0

8 0 0 0 0 0 0

7 0 0 0 0 0 0

6 0 0 0 0 0 0

5 0 0 0 0 0 0

4 0 0 0 0 0 0

3 0 0 0 0 0 0

2 0 0 0 0 0 0

1 0 0 0 0 0 0
1 4 .4 2 7
8 .0 0

1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

2 8 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A6

A b u n d a n c e

T IC : 9 5 B O R N E O L A 7 .D \ d a ta .m s
1 5 .0 5 4

1 .1 e + 0 7

1 e + 0 7

9 0 0 0 0 0 0

8 0 0 0 0 0 0

7 0 0 0 0 0 0

6 0 0 0 0 0 0

5 0 0 0 0 0 0

4 0 0 0 0 0 0

3 0 0 0 0 0 0

2 0 0 0 0 0 0

1 0 0 0 0 0 0
1 4 .4 4 3
8 .0 0

1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A7

2 8 .0 0

A b u n d a n c e
T IC : 9 5 B O R N E O L A 8 .D \ d a ta .m s
1 .1 e + 0 7

1 5 .0 5 2

1 e + 0 7

9 0 0 0 0 0 0

8 0 0 0 0 0 0

7 0 0 0 0 0 0

6 0 0 0 0 0 0

5 0 0 0 0 0 0

4 0 0 0 0 0 0

3 0 0 0 0 0 0

2 0 0 0 0 0 0

1 0 0 0 0 0 0
1 4 .4 3 4
1 0 .0 0

1 2 .0 0

1 4 .0 0

1 6 .0 0

1 8 .0 0

2 0 .0 0

2 2 .0 0

2 4 .0 0

2 6 .0 0

T im e - - >

Pic sc k GC-MS Mai hoa bng phin mu A8

2 8 .0 0