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B Y T

TRNG I HC DC H NI

NGUYN TH THU HOI

SNG LC CC CY THUC
VIT NAM C TC DNG C CH
XANTHIN OXIDASE IN VITRO
KHA LUN TT NGHIP DC S

H NI - 2014

B Y T
TRNG I HC DC H NI

NGUYN TH THU HOI

SNG LC CC CY THUC
VIT NAM C TC DNG C CH
XANTHIN OXIDASE IN VITRO
KHA LUN TT NGHIP DC S

Ngi hng dn
1. TS. Nguyn Thy Dng
2. TS. Nguyn Qunh Chi
Ni thc hin
B mn Dc lc
B mn Dc liu

H NI 2014

LI CM N

hon thnh kho lun ny, ti nhn c s gip v cng qu bu ca cc thy


c gio, bn b v ngi thn.
Trc tin ti xin by t li cm n chn thnh v lng bit n su sc ti TS. Nguyn
Thu Dng, TS. Nguyn Qunh Chi, TS. Nguyn Hong Anh, DS. Phm c Vnh - nhng
ngi thy lun quan tm, gip , tn tnh hng dn v ng vin ti trong sut qu trnh
hon thnh ti ny.
Ti xin chn thnh cm n ng y, Ban gim hiu cng ton th cc thy c phng o
to, cc b mn, phng ban khc ca Trng i hc Dc H Ni to iu kin cho ti hc
tp v tch ly nhng kin thc b ch trong sut 5 nm hc va qua.
Tip theo ti xin chn thnh cm n cc thy, c v cc anh ch k thut vin B mn Dc
lc nhit tnh gip , ng vin v to iu kin cho ti trong sut qu trnh nghin cu v
thc hin ti.
Ti xin gi li cm n n TS. Phng Thin Thng, Vin Dc liu; TS. Trn Th Bch,
Vin Sinh thi v Ti nguyn sinh vt Vit Nam; PGS.TS. Nguyn Th Hoi, khoa Dc, Trng
i hc Y dc Hu cung cp mu nghin cu ti thc hin ti.
Trong qu trnh tm kim ti liu v tra cu thng tin, ti cn nhn c s gip rt
nhit tnh ca cc anh ch ang hc tp ti nc ngoi trong Goup Nhm ti bo v Group
VietPhD.org. Ti xin chn thnh cm n s gip ht sc qu bu .
Li cui cng, ti xin gi li cm n n nhng ngi thn trong gia nh v bn b,
nhng ngi lun bn cnh v ng h ht lng, to iu kin tt nht, gip , ng vin v
ng gp kin ti hon thnh kho lun ny.
H Ni, thng 5 nm 2014.
Sinh vin
Nguyn Th Thu Hoi

MC LC
LI CM N
MC LC
DANH MC CC CH VIT TT V K HIU
DANH MC CC BNG
DANH MC CC HNH
T VN .............................................................................................................1
Chng 1. TNG QUAN ...........................................................................................3
1.1. TNG ACID URIC MU V CH TC DNG CA THUC .....3
1.1.1. Sinh tng hp v chuyn ha acid uric ...........................................3
1.1.2. T ng
1.1.3. h t

i uri m u ..........................................................................4
ng ca cc thuc h acid uric mu ................................6

1.2. ENZYM XANTHIN OXIDASE ...........................................................8


1.2.1. Vai tr ca xanthin oxidase trong tng hp acid uric .....................8
1.2.2. V tr, im l h v im cu trc ...................................8
1.2.3. Cc yu t nh hng n hot ng ca enzym xanthin oxidase .9
1.2.4. Mt s phng ph p x

nh hot ca enzym xanthin oxidase

............................................................................................................................10
1.3. CC NGHIN CU SNG LC DC LIU C TC DNG H
ACID URIC THNG QUA C CH XANTHIN OXIDASE IN VITRO ...........11
1.3.1. C

hng nghin cu trn th gii ..............................................11

1.3.2. Cc nghin cu Vit Nam ..........................................................14


Chng 2. NGUY N VT LIU, NI DUNG, PHNG TIN V PHNG
PHP NGHIN CU...............................................................................................17

2.1. NGUYN VT LIU .........................................................................17


2.1.1. Mu nghi n u ..........................................................................17
2.1.2. Chun b mu nghin cu..............................................................18
2.1.3. Thu , h

ht .............................................................................19

2.1.4. M y m , thit , ng ............................................................19


2.2. NI DUNG NGHIN CU................................................................19
2.3. PHNG PHP NGHI N CU .......................................................20
2.3.1. Nguy n t .....................................................................................20
2.3.2. Chun

ht

n thit .....................................................20

2.3.3. Tin hnh .......................................................................................21


2.3.4. Phng ph p x l s liu .............................................................23
Chng 3. KT QU V BN LUN ...................................................................24
3.1. KT QU ............................................................................................24
3.1.1. Kt qu sng lc tc dng c ch xanthin oxidase in vitro ca cc
mu c liu, thc vt ......................................................................................24
3.1.2. Kt qu x

nh IC50 ca cc mu c liu, thc vt tim n ng

............................................................................................................................39
3.2. BN LUN .........................................................................................41
3.2.1. V kt qu sng lc .......................................................................41
3.2.2. V kt qu c ch xanthin oxidase ca cc cy thuc tim n ng .46
KT LUN ...............................................................................................................50
XUT .................................................................................................................51
TI LIU THAM KHO
PH LC

DANH MC CC CH VIT TT V K HIU

BP dng

: B phn dng

CCTT

: Ch

Chng NK

: Chng nhim khun

: i th o ng

HA

: Huyt p

HGPRT

: Hypoxanthine-guanin phosphoribosyltransferase

DMSO

: Dimethyl sulfoxid

Oxh

: Oxy ha

PRPP

: Phosphoribosyl pyrophosphatase

TDKMM

: Tc dng khng mong mun

VQG

: Vn quc gia

XO

: Xanthin oxidase

thng tin

DANH MC CC BNG

Bng 2.1. B tr hn hp phn ng trong tng ging ...............................................21


Bng 3.1. nh hng ca cc mu c liu ln hot XO in vitro ti cc nng
100 g/ml, 50 g/ml, 10 g/ml .................................................................................25
Bng 3.2. nh hng ca cc mu thc vt ln hot XO in vitro ti cc nng
100g/ml, 50g/ml, 10 g/ml ...................................................................................34
Bng 3.3. Nng c ch 50% hot tnh enzym xanthin oxidase (IC50) ca cc mu
c liu, thc vt tim n ng ....................................................................................39

DANH MC CC HNH

nh 1.1. Qu tr nh h nh thnh

i uri trong th ..............................................3

Hnh 1.2. Cu trc khng gian ca xanthin oxidase ...................................................9


Hnh 2.1. Quy trnh th nghim nh gi t

ng c ch xanthin oxidase ca cc

mu th ......................................................................................................................22

T VN
T ng

i uri m u, c bit t ng

i uri m u mn tnh l nguyn nhn v

l yu t nguy dn n cc bnh l nh gt, t ng huyt p, bnh tim mch, bnh


thn, bnh i th o ng typ II [51], [82], [100]. Trong , gt l bnh l ri lon
chuyn ha, vim khp mn tnh ph bin nht v xu hng ngy cng gi t ng.
Cc thu iu tr t ng

i uri m u theo y hc hin i u h r

rng, tc dng nhanh v hiu qu iu tr tng i tt. Mt trong nhng h t


dng c l quan trng ca cc thuc h acid uric mu l xanthin oxidase, enzym
then cht trong on ng sinh tng hp acid uric. Tuy nhin, t ng

i uri m u l

bnh l ri lon chuyn ha nn bnh nhn thng phi dng thuc lu di. Trong
khi ,

thu tn thng c gi thnh

o v li cho bnh nhn nhiu

tc dng khng mong mun. V vy, vic tm kim ngun thuc thay th vi hiu
qu iu tr tng t ho vt tri hn, ng thi n ton hn

thu tn c

ng l hng nghin cu c quan tm pht trin.


Vic tm kim cc hp cht lm thuc t ngun nguyn liu thin nhin lun
l hnh s h u ti n trong hin lc pht trin ngnh Dc nhiu nc. Khai
thc ngun ti nguyn sinh hc t ngun c liu c s dng theo kinh nghim
trong nhn dn v sng lc tc dng c l ca dch chit t

c liu ny l

hng tip cn pht hin v pht trin cc thuc mi phc v iu tr [83].


Vn l mt t n thi n nhi n u i, nm trong vng nhit i gi
ma, Vit Nam c thm thc vt v ng

ng v phong ph vi nhiu loi h

c nghin cu v tc dng sinh hc, nn y hc c truyn pht trin t rt lu i.


Dn gian s dng rt nhiu cc bi thuc, v thu iu tr cc bnh v xng
khp nh thng phong (bnh gt theo y hc c truyn), phong thp... Cho n nay
ghi nhn nc ta c 3948 loi cy thuc, thuc 307 h ca 9 ngnh thc vt
b

o ng nh c thp (k c Nm) [15].


Li n qu n n tc dng c ch xanthin oxidase in vitro, trn th gii

nhiu nghin cu sng lc t ngun c liu v cc cht/nhm cht phn lp t


c liu c thc hin bi cc nhm nghin cu khc nhau [21], [46], [56],

[71], [76], [91], [110], [116]. Tuy nhin, ti Vit Nam, nghin cu theo hng ny
cn hn ch. Nguyn Th Thanh Mai v cng s sng lc c tc dng c ch
xanthin oxidase in vitro ca 96 c liu thu hi t 4 tnh min Trung b v min
Nam (Ph Y n, Kh nh H , Lm ng v An Giang) [85]. Nhm nghin cu ti b
mn Dc lc, Trng i h Dc H Ni trin kh i phng ph p nh
gi tc dng c ch xanthin oxidase in vitro tr n UV 96 ging Costar 3635 v p
dng sng lc c tc dng c ch xanthin oxidase in vitro ca 91 mu thc vt,
c liu thu hi cc tnh min Bc v min Trung Vit Nam [11]. Phng ph p
ny c trin khai vi nhiu u im ni bt to iu kin thun li cho cc
nghin cu sng lc tip theo. Vi m rng th m i tng c liu, thc vt
vo sng l t m kim, ph t hin
thit. V vy, ti S

loi hiu qu h
thu

xanthin oxidase in vitro th hin vi


1. Sng l t

ng

Vit N

i uri tt l rt
t

m ti u th s u:

h x nthin oxi se in vitro

mu

liu, th vt (khong 200 loi thu h i Vit N m.


2. X

nh IC50 cc mu liu, th vt tim n ng nht.

Ch g 1. TNG QUAN
1.1. TNG ACID URIC MU V CH TC DNG CA THUC
1.1.1. Si h t g hp v

hu h

i uri

Acid uric l sn phm chuyn ha cui cng ca

nu leo-protein h

nhn purin (adenin, guanin) trong th ngi vi s th m gi

enzym:

phosphoribosyl pyrophosphatase (PRPP), nucleoside phosphorylase, xanthin


oxidase (XO) [36], [41] (H nh1.1 . Trong , c base purin (adenin v guanin) l
sn phm trung gian ca qu trnh thoi ha cc acid nucleic (ADN, ARN). Tip
theo, adenin v guanin b thy phn gii phng nhm amin to thnh hypoxanthin
v x nthin, i tc dng ca enzym xanthin oxidase hypoxanthin b oxy ha thnh
xanthin ri thnh acid uric [3].

nh

. Qu tr nh h nh thnh

i uri trong th [41]

h u ht

loi ng vt v (tr loi ngi v

loi linh trng ,

ui on ng huyn h purin th m uri se, mt loi enzym g n v i tr


huyn h

i uri thnh ll ntoin, l ng t n hn v o thi r ngoi

theo thn [9], [40], [96].


A i uri thi tr qu r ngoi th theo h i on ng: khong 2/3
lng

i uri to r mi ngy thi tr qu thn, 1/3 thi tr qu rut

[58], [63].
1.1. . T

i uri

B nh thng nng

i uri trong m u lun gi n nh m

7,0 mg/ l (420 mol/l i vi n m v 6,0 mg/ l (360 mol/l i vi n o s


n ng gi qu tr nh tng hp v o thi [12], [36]. Bt k nguy n nhn no
lm mt n ng qu tr nh ny (t ng tng hp ho gim thi tr u lm t ng
i uri trong m u. T ng

i uri m u x

nh khi nng

id uric mu

vt qu 7,0 mg/ l (420 mol/l i vi n m v 6,0 mg/ l (360 mol/l i vi n


[12].
2

Nguyn nhn gy tng acid uric mu

Gim o thi acid uric qua thn


- Gim kh n ng i xut
nhn,

i uri ng thn ti n ph t o nhiu nguy n

it trong trng hp nghin ru [41], [63]. Loi ny thng tnh

ht gi nh, khi ph t thng o ung qu nhiu ru. y l nhm h y gp nht


(90%

trng hp [63].
- Gim kh n ng i xut acid uric thn do s dng thuc hoc suy thn

dn n ri lon ch n ng thn. Cc thu thng gp l aspirin liu thp;


phenylbutazone liu thp;

s cc thuc li tiu dng ko di (tr nhm

spironol tone nh thi zi e, furosemi e; thu

iu tr lao nh eth m utol,

pyrazinamind; cc thuc acid ethacrynic, acid nicotinic... [40], [99].


Tng sn xut acid uric
- T ng to

i uri nguy n ph t ( m sinh) l nhm nguy n nhn him

gp ( i 1% ; o ri lon hot ng

enzym th m gi vo qu tr nh huyn h

purin nh t ng hot tnh PRPP v x nthin oxi se ho thiu ht mt ph n h y


ton ph n HGPRT (enzym x t

ho phn ng ngh h huyn

i gu nyli v

hypoxanthin thnh xanthin) [12], [36], [41].


- T ng to
nhn purin (

it

i uri th ph t ( him khong 10% : o n nhiu th


tht mu nh h, ,

gp trong nh thiu m u t n m u, nh h
ng ho

; hi sn...); o t ng hu t bo,

u (leucemia), nh u ty xng,

ht gy t bo trong iu tr ung th; nh vy nn...[36], [41].

1.1.2.2. Hu qu ca tng acid uric mu


Tng acid uric mu v bnh gt
Khi nng acid uric trong huyt th nh

o hn nng bo ha dn ti

t h ly tinh th urat ti cc m, to nn cc ht microtophi. S lng ng ca cc


ht ny cc v tr khc nhau gy ra cc tn thng kh

nh u. Khi cc ht tophi ti

sn khp b v s khi ph t n gt cp; s lng ng vi tinh th cnh khp, mng


hot dch khp, trong m sn v m xng s dn n bnh xng khp mn tnh
do gt; cc vi tinh th urat lng ng ti m mm, bao gn to nn ht tophi [12],
[40].
T ng

i uri m u l yu t nguy , l nguy n nhn

i uri m u ng

o, thi gi n t ng

nh gt cng cao [128].

nh gt [40]. Nng

i uri m u ng ko i th nguy

Tng acid uric mu v cc bnh khc lin quan


G n y, nhiu cc nghin cu lm sng h ra rng t ng acid uri m u
mn tnh lm t ng nguy mc bnh thn, nh t ng huyt p, bnh tim m h, nh
tiu ng [51], [82], [96], [118]. Cc nghin cu can thip g n y ng ung p
bng chng cho thy, gim urat khi s dng allopurinol c th lm chm tin trin
ca suy thn mn tnh, bnh tim mch [40], [52], [96].
Nh vy, h
nh l kh

i uri m u l m ti u iu tr quyt nh trong nh gt v

li n qu n ti t ng

i uri .

1.1.3. h t

thu

i uri

H i h ca cc thuc iu tr t ng

u
i uri m u hin nay l c ch

xanthin oxidase hoc gy thi tr acid uric qu nc tiu. Ngoi ra, nhm thuc tiu
i uri

ng ng trong gi i on th nghim lm sng, tuy nhin nhm thuc ny

gp phi nhng hn ch nht nh n n h c ng dng trong iu tr t ng

uric mu mn tnh.
1.1.3.1. Gim tng hp acid uric thng qua c ch enzym xanthin oxidase
Allopurinol v fe uxost t l h i thu in h nh t
ng s

ng trong iu tr uy tr

ng

h XO hin

nh gt mn tnh.

Allopurinol
- C ch tc dng: Allopurinol t
v th t

ng nh mt ht

- Ch nh: Allopurinol s
nh gt mn tnh v
xut

tin ht

thn hn

ng nh ht

h nh tr nh enzym

h khng nh tr nh nng
ng h nng

trng hp t ng

o [2], [92].

i uri m u trong iu tr

i uri m u [2]. Thu lm t ng i

i uri qu n tiu n n t gy si thn v n u qun

thu t ng o thi

i uri qu thn [2]. V vy, thu

qu n trng vi nhng nh nhn khng p ng vi

v i tr

thu t ng o thi

uri v nh nhn si ur t [2], [23].


- Tc dng khng mong mun: Vi m g n, tn thng thn, ng/hi hng qu
mn v lm t ng tnh 6-mercaptopurin [2], [67].
Febuxostat
Febuxostat l thuc mi r i sau allopurinol, g n y l n lt C
quan qun l thuc chu u - EMEA v FDA ph duyt iu tr di hn t ng
acid uric mu mn tnh trn bnh nhn gt. So vi allopurinol, febuxostat c
chng minh lm gim ucid uric mu hiu qu hn trong th nghim tin lm
sng v lm sng [22].

- C ch : Fe uxost t h

i uri theo h

h khng nh tr nh

enzym xanthin oxidase [42].


- Ch nh : Febuxost t l l

hn th h i s u llopurinol khi m nhng liu

ph p gy t ng thi tr khng th s

ng tr n nhng nh nhn m

nh

thn mn tnh, si thn [100].


- Tc dng khng mong mun : Gy ri lon h n ng g n, u u, un nn,
hng mt; nguy
s

ng h nhiu,

nh tim m h [42]. Fe uxost t l thu mi, kinh nghim


TDKMM

n tip t theo i.

1.1.3 2 Tng thi acid uric thn


C

thu gy t ng thi

tt trong vi lm h

i uri nh pro ene i , sulfinpyr zon hiu qu

i uri m u,

it l gim k h th v s lng

ht

tophi [9]. Tuy nhi n, thu t r km hiu qu vi nh nhn suy gim h n ng


thn v th gy si ur t v nhiu t

ng khng mong mun kh

[100]. Hin n y, nhm thu ny khng n s

[2], [80], [97],

ng ph in tr n lm sng.

1.1.3.3. Tiu acid uric


H u ht

loi ng vt c v, tr on ngi v cc loi linh trng trong

th u c enzyme uricase, mt loi enzym chuyn ha acid uric thnh allantoin


d t n v o thi tt hn [40], [97]. V vy, truyn tnh m h uri se c
chng minh c tc dng phn gii acid uric [90]. h t

ng ny m r hng

iu tr mi cho nhng bnh nhn khng p ng vi cc thu iu tr t ng

uric mu mn tnh ng hin nay.


Rasburicase: l mt uricase ti t hp, c FDA cho php s dng h
acid uric ngn ngy trn trn tr em mc hi chng phn gii khi u (tumour lysis
syndrome) vo n m 2002 [115]. Tuy nhin qu trnh s dng r s uri se ko i
p ng yu c u iu tr t ng

i uri m u mn tnh b hn ch bi tnh khng

nguyn cao, t1/2 ngn v kh n ng dung np km.

PEGylated uricase: l mt dng dn cht c uri se (c gn thm chui


polyethylen gly ol lm gim tnh khng nguyn v ko di thi gian bn thi
hn so vi uricase [49].

1.2. ENZYM XANTHIN OXIDASE


1.2.1. V i tr x

thi oxi

se tro g t g hp

i uri

Xanthin oxidase l mt enzym c vai tr then cht trong on ng to ra


i uri . C

protein nhn purin c chuyn ha thnh hypoxanthin v xanthin,

i tc dng xc tc ca enzym XO chng c oxy ha thnh acid uric. Enzym


XO c vai tr quan trng trong qu tr nh iu ha nng acid uric mu, bt k tc
ng no nh hng n hot ng c XO ng nh hng n s to thnh acid
uric [3], [20]. V vy, enzym XO l mt trong nhng h t

ng c l chnh

m cc thu iu tr gt ng hng ti.


1.2.2. V tr, i

v i

u tr

1.2.2.1. Ngun gc, phn b


Enzym XO t m thy nhiu loi ng vt v, him, n trng v vi
khun [14]. Trong c

loi ng vt v, enzym XO phn rng ri trong

m nhng nng
trong h u ht

o nht l g n v rut [25]. ngi XO t m thy

t o

th, tuy nhi n n nhiu nht trong t o g n v

t o rut [20]. Enzym XO dng trong cc nghin u hit xut t s b l


hiu nht, ng thi ng th hit t g n hut, g v t tuyn v
hut [54].
1.2.2.2. c im cu trc
Xanthin oxi se l mt protein khi lng khong 300 kD , gm 2 tiu
n v. Mi tiu n v gm 4 trung tm oxy h kh : mt ph n ph moly en, mt
FAD v hai Fe2S2 [20],[54]. Ph n ph molybden bao gm mt dn xut pterin hu
li n kt cng ha tr vi 2 nguyn t lu hunh ca molybdopterin, 2 nguyn t
oxy v 1 nguyn t lu hunh khc [54] (Hnh 1.2).

Hnh 1.2. Cu tr khng gi n x nthin oxi se [92]


(A: Cu trc khng gian ca xanthin oxidase, B: Phn ph molybden)
1.2.3. C

u t h h g

hot g e z

xanthin oxidase

- Nng c cht: Khi nng ht xanthin t ng th t phn ng


huyn h s t ng, lng
no , enzym s o h

i uri to r s ng nhiu. Tuy nhi n, n mt m


ht v t phn ng khng t ng thm [3].

- Nng enzym: Khi nng enzym t ng l n, t phn ng ng t ng.


Tuy nhi n, khi nng enzym t ng n mt m nht nh, sn phm to r qu
nhiu s t

ng vo trung tm lp th enzym, phn ng o h v t

phn ng s khng t ng l n n [3].


- Nhit : Khi t ng nhit l n 100C, t phn ng s t ng l n 2 l n.
Tuy nhi n, n mt nhit qu

o, enzym s in tnh o ph v

li n kt

trong u tr . Theo mt s nghi n u, nhit ti u ho x nthin oxi se t s


l 100C v ho x nthin oxi se t s

l 200C [44], [65]. Nhit ng l mt

yu t qu n trng trong qu tr nh o qun enzym. X nthin oxi se o qun


nhit 40C s khng gim hot tnh trong vng 6 th ng.
- pH: Mi enzym mt gi tr pH ph hp nht ho hot ng enzym
. Vi x nthin oxi se, pH th h hp t 7,5 8,0 [65].

10

- Cc yu t khc: mt s ion kim loi nng (V : Hg2+, Ag+ , mt s


ht

h (purin, pyrimi in, mt s h hit liu

ng th lm gim

hot tnh enzym [3], [14].


1.2.4. Mt s ph g ph p x

h hot e z

thi oxi

se

Phng php o quang


-

Phng ph p o qu ng tr n nh lng
oxy h x nthin i x t

XO theo phn ng :

Xanthin + H2O + O2
Lng

i uri to thnh t qu tr nh

Acid uric + H2O2

i uri to thnh t l thun vi hot enzym v nh

lng ng phng ph p o qu ng sng 290nm [89].


-

Phng ph p o qu ng

tr n

ht ABTS (2,2-azino-di (3-

ethylbenzothiazoline-6-sulphonate)
Nguyn tc chung : Hot x nthin oxi se x
ABTS ng oxy h to thnh theo

phn ng:

Hypoxanthin + 2H2O + 2O2


Acid uric + O2
H2O2 + ATBSred

nh thng qu lng

Acid uric + 2H2O2

Allantonin + H2O2 + CO2

ATBSOX + 2H2O

Hot x nthin oxi se x

nh thng qu vi o hp th

ATBSox to thnh s u 10 pht sng 410nm [79].


Phng php o p
X nthin oxi se l mt ht nhng ele tron n n th x
enzym thng qu vi nh gi s kh

nh hot

ht nhn ele tron ph hp nh oxy,

x nh methylen v yto rom C. V vy, th x

nh hot enzym thng

qua t l oxy ti u th vi ht x nthin ng phng ph p o p 370C [39].

11

Phng php sc k lng hiu nng cao vi detector hunh quang


Nguyn tc : tr n phn ng oxy h 2-amino-4-hydroxypteridin (AHP)
thnh isox nthopterin (IXP

i s x t

x nthin oxi se. Lng IXP to r

t l vi hot x nthin oxi se phn t h qu

t s k v ph t hin,

nh lng ng ete tor hunh qu ng vi sng k h th h v sng ph t


hin tng ng l 343nm v 410nm [103].

1.3. CC NGHIN CU SNG LC DC LIU C TC DNG H


ACID URIC THNG QUA C CH XANTHIN OXIDASE IN VITRO
Xanthin oxidase l mt trong nhng h t
thu h

i uri hng ti. Nghi n u sng l in vitro l u t m kim

v ph t hin
t

ng l qu n trng m

liu tim n ng, t th nghi n u theo nh hng

ng sinh h v nh gi su hn khng nh t

tr n ng vt th nghim

ng tr n m h nh gy nh

liu kh n ng

h x nthin oxidase

mnh nht. T , to tin ho vi nghi n u v ph t trin thu mi.


1.3.1. C

h g ghi

Li n qu n n t

u tr th gii

ng

nghi n u sng l

h x nthin oxi se in vitro, tr n th gii nhiu

liu,

nhm ht/hot ht phn lp t liu,

h yu theo 3 hng tip n hnh :


Tip cn sng lc da trn kinh nghim s dng dc liu trong nhn dn
hoc nn Y hc c truyn ca mt s nc
Hng tip cn ny kh ph bin v c thc hin nhiu nc trn th gii.
N m 1999, d tr n kinh nghim s

ng liu trong iu tr gt v

triu hng li n qu n th n B M , Owen v ng s tin hnh sng l


kh n ng

h XO in vitro 26 loi thu 18 h th vt. Kt qu ho thy,

m liu 100 g/ml, 20% s loi th


enzym, trong Larix laricina t

ng

h tr n 50% hot tnh

ng mnh nht (86,33% [91].

12

N m 2000, Kong v cng s sng l 122 liu ng trong Y h C


truyn Trung Qu v ph t hin 69 h hit meth nol th hin tc dng c ch
enzym XO (56,56%), trong 29 ch chit c ch trn 50% hot tnh enzym
nng 100 g/ml (23,77%); 40 h hit n

ng

h XO (32,79%),

trong 13 ch chit c ch tr n 50% nng 100 g/ml (9,84%). nng


50 g/ml, c 58 dch chit methanol (47,54%), 24 dch chit nc th hin tc dng
c ch (19,67%); trong 15 dch chit methanol (47,54%) v 2 dch chit nc c
ch XO trn 50% (1,64%). Dch chit nc ca Polygonum cuspidatum t

ng

mnh nht (IC50 = 38 g/ml), tuy nhin vn yu hn nhiu so vi allopurinol (IC50 =


1,06 g/ml) [71].
N m 2001,

nh kho h Austr li nghin cu trn 28 dch chit t 17

loi thc vt bn c nhn dn s dng vi tc dng chng vim. nng


100 g/ml, c 9 loi th hin tc dng c ch XO; nng 50 g/ml c 4 loi th
hin tc dng c ch XO v pht hin

h hit Clerodendrum floribundum

(Verbenaceae), Eremophila maculata Ker Gawler (Myoporaceae) v Stemodia


Grossa Benth. (Scrophulariaceae), Stemodia grossa t

ng mnh nht vi IC50

< 50 g/ml [110].


N m 2007, nghin cu sng lc tc dng c ch XO c tin hnh vi 18
dch chit nc, methanol v hn hp nc - methanol t 6 c liu c ngi
dn bn a n s dng theo kinh nghim iu tr bnh gt v cc triu
chng lin quan. Kt qu cho thy 14 dch chit th hin tc dng c ch XO
nng 100 g/ml, trong 10 ch chit c tc dng c ch ln hn 50% hot
tnh XO v IC50 < 100 g/ml. Bn dch chit methanol th hin tc dng c ch XO
in vitro mnh nht: Coccinia grandis (IC50 = 29,79 g/ml), Datura metel (IC50 =
76,75 g/ml), Strychnos nux-vomica (IC50 = 6,8 g/ml) v Vitex negundo (IC50 =
78,75 g/ml vo nh gi tc dng h acid uric in vivo trn m hnh gy
t ng

i uri m u p bng kali oxonat [116].


N m 2010, H vlik v

ng s tin hnh nghin cu sng lc tc dng

c ch XO in vitro ca 84 dch chit ethanol v methylen clorid-methanol t 27

13

c liu c s dng theo kinh nghim iu tr bnh gt, vim khp hoc
bnh thp khp ca Cng ha Sc v khu vc min Trung - ng u. Kt qu cho
thy, khong 50% s loi th hin tc dng c ch XO nng 200 g/ml. Trong
th hin tc dng mnh nht l dch chit methylen chlorid-methanol ca
Populus nigra (IC50 = 8,3 g/ml) v Betula pendula (IC50 = 25,9 g/ml), dch chit
ethanol ca Caryophyllus aromaticus v Hypericum perforatum vi IC50 < 50 g/ml
[56].
N m 2011, cc tc gi Phillipines nh gi kh n ng c ch XO ca dch
chit t 15 c liu s dng vi tc dng chng vim trong y hc c truyn, kt
qu cho thy 5 c liu (33,33%) c tc dng c ch trn 50% hot tnh XO
nng 100

g/ml nhng t

ng yu hn rt nhiu so vi thu i chng

allopurinol [21]. Cng n m , Hudaib v cng s tin hnh nghin cu trn


dch chit methanol t 23 dc liu thuc 12 h thu hi vng Jordan, kt qu cho
thy 3 c liu (13,04%) c tc dng c ch trn 50% nng 200 g/ml
[59].
Tip cn sng lc theo h hoc chi thc vt
Mt s tc gi la chn hng tip cn sng lc theo h hoc chi thc vt
nh gi tc dng c ch XO. Hng nghin cu ny tp trung nghin cu su theo
mt h, mt s h hoc chi nht nh tm kim v pht hin cc cy thuc c
tim n ng nht. C

c liu thuc cc h c cc nh nghin cu vo sng

lc nhiu nht, bao gm: h Sim (Myrt e e [113], h Dy gi (Celastrace e v


h Bc h (Lamiaceae) [53] v g n y l

c liu thuc chi Lychnophoras h

C (Aster e e [46].
D liu thu

h ny thng s

c truyn ca nhiu n vi t

ng ph in trong y hc

ng hng vi m, gim u ng thi h

fl vonoi , nhm hp ht nhiu hot tnh sinh h trong t


oxy h , c ch xanthin oxidase.

ng hng

14

Tip cn theo nhm hp cht t nhin phn lp t dc liu


C

nhm hp

ht

tim n ng

h x nthin oxi se nh fl vonoi ,

polyphenol, oum rin h y t nin sng l t


t m kim

ht t

ng

fl vonoi vi rt nhiu

h mnh nht [27], [29], [38], [40]. in h nh l

nghi n u.

N m 2002, nghi n u li n qu n u tr t
Lim v ng s l
tng t

ng tr n m h nh in vitro

hn

pigenin t

ng sinh h
ng

fl vonoi ,

h XO mnh nht o

thun li vi trung tm hot ng enzym [76]. N m 2011, kt hp vi

nghi n u u tr , nh gi tng t
enzym, Li v ng s l
s 2092 fl vonoi

khng gi n gi phn t hp ht vi

hn 2 fl voni

ng tt nht (trong tng

liu) l: myricetin (IC50=62,7g/ml), luteolin

(IC50=3,29 g/ml) [74]. N m 2013, Hum m hesw ri nh gi t

ng

XO in vitro 6 hp ht fl vonoi : sepigallocatechin, acacatechin, myricetin,


naringenin, daidzein n gly itein, kt qu ho thy 6 hp ht ny u t
ng

h XO tt vi IC50 t 22-62 g/ml nh n ng lng li n kt thp vi

trung tm hot ng enzym ( h


hin t

h nh tr nh ; trong gly itein th

ng mnh nht vi IC50 t 24 0,28 g/ml [117].

1.3.2. C

ghi

u Vit N

Ti nguy n th vt Vit N m rt phong ph v


truyn ph t trin t rt lu i, nhiu loi s
gi n vi m h h
thu mi [106]. C
sng l t

ng

ng, nn y h

ng theo kinh nghim n

nh, nhiu loi tnh hu, tim n ng ph t trin

nh kho h Vit N m ng th hin


h XO in vitro

kt qu kh qu n. Tuy nhi n

nghi n u

mu th vt, liu, u ho

nghi n u theo hng ny n kh hn h.

N m 2004, phi hp vi trng i h Y D Toy m (Nht Bn , t


gi Nguyn Th Th nh M i v ng s nhm nghi n u ti Kho H , trng
i h Kho h t nhi n thu i h Qu gi thnh ph H Ch Minh th
hin nghi n u theo nh hng sng l

liu tim n ng

h XO t

15

ngun liu Vit N m phn lp


liu hot tnh
96 liu s
nh gt v

ht hot tnh sinh h t

h mnh nht. Nghi n u sng l 288 h hit t

ng trong y h

truyn v theo kinh nghim n gi n iu tr

triu hng li n qu n, h yu thu h i 4 tnh min N m Vit N m

(Ph Yn, Kh nh H , Lm ng v An Gi ng). T

ng

h XO in vitro

nh gi 4 nng 100 g/ml 50 g/ml 25 g/ml 10 g/ml. Kt qu ho


thy, nng 100 g/ml 46 h hit (15,97%)
enzym; 21 h hit (7,29%) t

ng

50 g/ml; 8 h hit (3,51%)


g/ml; 2 h hit (0,69%) t

h tr n 50% hot tnh

h trn 50% hot tnh enzym nng

h tr n 50% hot tnh enzym nng 25

ng

h tr n 50% hot tnh enzym nng

10 g/ml. Trong h hit meth nol 4 loi Artemisia vulgaris, Caesalpinia


sappan, Blumea balsamifera, Chrysanthemum sinense v
methanoln

Tetracera scandens t

g/ml [85]. T kt qu sng l


u h th vt t

ng

hit trong

h mnh nht vi IC50 < 20

n u nhm nghi n u tip t tin hnh nghi n

liu tim n ng. N m 2005, nghi n u h th vt t

thn g Caesalpinia sappan phn lp 17 hp ht t nhi n trong 9


hp ht t
t

ng

ng

h XO vi IC50

h mnh nht l s pp n h l one vi IC50 = 3,9 M [84]. N m 2006,

nghi n u h th vt t ho
fl nvon gly osi e mi l

o ng t 3,9 n 93,2 M. Hp ht c

Chrysanthemum sinense phn lp 1

etin v 27 hp ht fl vonoi it kh , n ht

ffeoyl quini , monoterpenoi gly osi . C

h XO mnh vi IC50 dao ng t 0,13-2,31

hp ht ny hot tnh
M, thm

h mnh hn

allopurinol (IC50 = 2,5 M) [86]. G n y nht, n m 2012, t liu Tetracera


scandens phn lp 1 hp ht nor-lup n triterpen mi kh n ng

XO mnh (IC50 = 1,9 M) so vi llopurinol IC50 = 2,5 M) [87].


N m 2013, nhm nghi n u ti mn D l , Trng i h D H
Ni trin kh i phng ph p nh gi t
vitro tr n UV 96 ging v p ng sng l t
th vt, liu thu h i ti

ng

h x nthin oxi se in

ng

h XO 91 mu

tnh min B v min Trung Vit N m. C

16

liu vo sng l s
ho

ng theo kinh nghim trong iu tr gt

ng li n qu n nh hng vi m, gim u, hng oxy h . C

liu nh gi t

ng

h XO 3 nng 100 g/ml 50 g/ml

10 g/ml. Ti nng 100 g/ml, 68 mu th th hin t


trong 11 mu
ng

mu

ng

h XO,

h trn 50%. Ti nng 50 g/ml, 38 mu th hin tc

h XO, 2 mu

h tr n 50% hot tnh enzym v khng mu no

h tr n 50% hot tnh enzym nng 10 g/ml. Trong , 4 liu tim n ng


nht l Smilax perfoliata Lour. (thu h i ti vn qu gi B h M , Clinacanthus
nutans (thu h i ti S P - Lo Cai), Homalomena occulta Lour s hott (thu h i ti B
V- H Ni , Archidendron clyearia (J k. , I. Niels (thu h i ti Qung Tr . Mu
kh n ng

h x nthin oxi se in vitro mnh nht l M n (Archidendron

clyearia (Jack.), I. Niels vi IC50 l 15,6 g/ml [11].


Trong ti ny, nhm nghi n u tip t m rng
sng l t m kim, ph t hin th m

liu t

i tng vo
ng

h XO mnh

theo h i hng tip n.


Mt l,

liu ho

liu thu cc chi th vt

ng ng tr nh xng khp, t
ho

ng h

i uri ,

h XO, h

it n
i uri

ng li n qu n nh hng vi m, hng oxy ha thu thp t n

Bo tn ngun y thu

truyn Vin D liu [4],

y thu

ng o n t P ko-Vn Kiu, Qung Tr.


Hai l,

mu th vt h

n Tim n ng sinh h

thng tin v t

ng sinh h thu thp t

ngun nguy n liu sinh h Vit N m Vin

Sinh th i v Ti nguy n sinh vt [1].

17

Ch g . NGUY N VT LIU, NI DUNG, PHNG TIN


V PHNG PHP NGHI N CU
2.1. NGUY N VT LIU
2.1.1. Mu ghi

ti tin hnh sng l 244 mu liu, th vt thu h i Vit N m theo


h i hng tip n sng l :

47 mu dc liu c r sot thng tin, c thu thp t cc ngun:

nh

- D n Bo t

gu

nh t n kho h

mu liu o

liu x

thu

tru Vin D liu [4]. Vic


nhn Ng V n Tri, Vin D

nh. Mu ti u n lu gi ti Vin D liu.


-C

y thu

ng o n t P ko, Vn Kiu Qung Tr v

ng ng phng v h

nh li n qu n n t

ng hng oxy ho . C

mu

cy thu h i i PGS.TS Nguyn Th Hoi Kho D i h Y D


Hu thu h i v PGS. TS. Ninh Kh Bn, ThS. Nguyn Th Cng Vin
Sinh th i v Ti nguy n Sinh vt Vin Kho h v Cng ngh Vit N m x

nh

t n kho h . Mu ti u n lu gi ti Kho D H Y D Hu.


Ti u h l

hn liu vo

nh s h sng l :

liu m hi ghi nhn t

h x nthin oxi se, h


-

ng hng vi m, hng oxy h ,

i uri , iu tr nh xng khp.

s liu s

+C

liu ho

ng r so t thng tin v l

ti liu huy n ngnh Th vt D liu D

hn:
truyn: Nhng

cy thuc v v thuc Vit Nam Tt Li, Cy thuc Vit Nam V V n


Chi v

o iu tr v tri th s

ng thu

nhn n

phng

[6], [13], [16].


+C
i uri

nghi n u v t

ng hng vi m, hng oxy h ,

mu liu thu ho

h XO, h

hi liu

ng

18

[8], [10], [19], [24], [28], [31], [32], [33], [34], [37], [43], [45], [47], [48], [55],
[60], [61], [62], [64], [68], [69], [70], [72], [75], [77], [81], [88], [91], [93], [95],
[98], [99], [101], [104], [105], [108], [111], [112], [114], [119], [120], [124], [125],
[126], [127], [129], [130], [131].
Danh sch 147 mu liu l

hn vo sng l t

ng

h x nthin oxi se in vitro th hin trong ph l 1.


97

u th vt h

D n Ti

th g ti v t

g si h h

gu

g, thu thp t gu :

gu

iu si h h Vit N

Vin Sinh th i v Ti nguy n sinh vt o nhm nghi n u TS.Tr n Th B h


thu thp ti nhiu vng sinh th i kh
C

mu th vt ny h

nh u Vit N m [1].

thng tin v t

D nh s h 97 mu th vt l

ng sinh h , ng ng.

hn vo sng l t

ng

xanthin oxidase in vitro th hin trong ph l 2.


2.1.2. Chu b

u ghi

- Chun b mu cao dc liu, thc vt: Cn 100 g mu liu sy


kh ho vo

nh

u 1 lt, thm m vi MeOH ri tip t MeOH ngp

liu khong 3 m. Chit (2 l n hi lu

h thu trong 2 gi, l qu giy l ly

h hit. Ct thu hi ung mi, sy hn khng thu


hit xut

o ton ph n

ung p mu:

o ton ph n. Vi

mu liu, th vt th hin ti ni

mu liu

hit xut ti Kho Phn t h, Vin D

liu v i nhm nghi n u PGS. TS Nguyn Th Hoi, Kho D , Trng


i h Y D Hu;

mu th vt

hit xut ti Vin sinh th i v Ti

nguy n sinh vt i nhm nghi n u TS.Tr n Th B h.


- Chun b mu th in vitro: C o ton ph n tng mu liu, th
vt x

nh hm m, h t n trong ung mi dimethyl sulfoxid (DMSO

ung h g nng 10 mg/ml. T ung h g ph long ng m

phosphat pH 7,5 thnh

nng th h hp th hin

phn ng enzym.

19

.1.3. Thu , h

ht

- Thu i hiu: llopurinol (Sigm Al ri h .


- X nthin 99% (Sigm Al ri h .
- Xanthine oxidase t sa b (0,8 U/mg protein, 9 mg protein/ml, Sigma
Aldrich).
- H

ht ph mu liu: dimethyl sulfoxid (Merck).

- H

ht ph m N 2HPO4.2H2O, KH2PO4, HCl, NaOH (Merck) t tiu

chun phn tch.


.1. . M

, thi t b, g

- Cn phn tch AY 220 (SHIMADZU).


- My o pH (EUTECH .
- My ly tm (HERMLE Z300).
- My siu m (Power sonic 405).
- My ct nc 2 l n (Halminton, Hoa K).
- Thit b khuy t (Hei olph, c).
- T iu nhit (Memmert).
- T sy (Memmert).
- H thng ELISA gm m y c khay vi tinh th (Biotek, Hoa K) v my
lc khay (Awareness, Hoa K).
- UV 96 ging y phng Costar 3635 (Corning).
- Mi ropipet mt u k nh v k nh vi

loi th tch: 0,5-10 l,

2-20 l, 10-100 l, 100-1000 l, 1000-5000 l (Eppendorf).


- Microtube cc loi th tch: 0,5 ml, 1 ml, 1,5 ml, 2 ml, 5 ml (Eppendorf).

2.2. NI DUNG NGHI N CU


th hin
- Th hi

m ti u r , ti thit k vi
tiu 1: Sng l t

h x nthin oxi se in vitro


Chun mu th in vitro.

ng h

mu th vt, liu.

ni ung s u:
i uri thng qu

20

nh gi t

ng

h x nthin oxi se in vitro

o ton ph n

liu, th vt 3 nng 100 g/ml, 50 g/ml v 10 g/ml l


hn r

liu tim n ng.

- Th hi

tiu : X

nh IC50

liu tim n ng

h x nthin oxi se .
Kho s t kh n ng
nh u x

nh IC50. T l

mu th tim n ng
hn r

liu t

nng kh
ng mnh nht.

2.3. PHNG PHP NGHI N CU


p ng phng ph p nh gi t

ng

h x nthin oxi se in vitro tr n

UV 96 ging Cost r 3635 trin kh i i nhm nghin u ti mn


D l , trng i h D H Ni trong t

gi l thnh vi n nhm

nghi n u [11]. Phng ph p ny trin kh i theo phng ph p


Noro trong ng nghim, Nguyn Th Th nh M i tr n 96 ging v
iu hnh ph hp vi iu kin phng th nghim [85], [89].
2.3.1. Nguyn t
D tr n s to thnh
oxidase:

Xanthin + H2O + O2

Lng

i uri t x nthin nh s x t

x nthin

Acid uric + H2O2

i uri to thnh t l thun vi hot enzym v nh lng

ng phng ph p o qu ng 290nm. Mu ht th so s nh vi mu
khng ht th nh gi t

ng

2.3.2. Chu b

thi t

ht

h enzym

ht th .

- m phosph t 70mM, pH = 7,5.


- Enzym x nthin oxi se 0,01 U/ml trong m phosph t.
- C ht: ung h x nthin 150 M trong m phosphat.
- H

ht ng phn ng: ung h HCl 1N.

- Allopurinol (nng 0,5 g/ml) lm i chng ng.

21

2.3.3. Ti
2.3.3

nh gi nh hng ca cc mu cao dc liu, thc vt ln hot

xanthin oxidase in vitro


B tr th nghim:
Trn mi UV 96 ging Costar 3635 gm c: ging hng, ging i hiu
(allopurinol) v cc ging th . B tr hn hp th trong

ging th hin

ng 2.1.
B g .1. B tr hn hp phn ng trong tng ging
Gi

g h g

Gi

g i hi u

gi

g th

Dung h th

50l

Allopurinol 0,5 g/ml

50l

m phosph t pH 7,5

85l

35l

35l

Xanthin 150 M

60l

60l

60l

XO 0,01 U/ml

30l

30l

30l

HCl 1 N

25l

25l

25l

Tng th t h

200l

200l

200l

Quy trnh th nghim


Qui trnh tin hnh nh h nh 2.1.
-

Mu th l

o ton ph n

liu ph trong m 3 nng

10 g/ml, 50 g/ml, 100 g/ml t ung h g nng 10 mg/ml trong


DMSO.
-

Song song vi mi mu hng, mu i hiu, mu th

mt mu trng

hng, trng i hiu v trng th tin hnh tng t

nhng th y i tr nh t ho enzym vo ging (enzym

ho vo s u HCl

1N).
-

Th nghim lp li 3 l n (Mi nng : th 3 l n/ngy, trong 3 ngy


khc nhau).

22

Hnh 2.1. Quy tr nh th nghim nh gi t


nh gi kt qu:
Tnh I (%

h theo ng th :

Trong :
OD: hp th qu ng
OD hng = OD hng ODtrng
ODth

= ODth ODtrng th

hng

ng

h XO

mu th

23

2.3.3.2. Xc nh IC50 ca cc mu thc vt, dc liu c tim nng c ch XO


T

ng

h XO

y nh gi theo tr s IC50, l gi tr nng

(tnh ton theo l thuyt ti mu th

h 50% s to thnh

i uri trong

hn hp phn ng so vi mu hng [9].


Vi tng mu th l

hn, thit lp y nng kho s t, t x

nh

IC50 v gii hn khong tin y.


B tr th nghim: nh m t m 2.3.3.1.
-

Mu th l

mu th vt, liu tim n ng nht ph

trong m thnh mt y 5 ho 6 nng kh


-

nh u.

Song song vi mi mu hng, mu i hiu, mu th

mt mu trng

hng, mu trng i hiu, mu trng th tin hnh tng

t nhng th y i tr nh t ho enzym vo ging (enzym

ho vo s u

HCl 1N).
-

Th nghim lp li 3 l n.

nh gi kt qu:
-

Tnh I (%

h theo ng th (1).

nh nng

h 50% hot x nthin oxi se (IC50

2.3.4. Ph g ph p x s

tng mu.

iu

S liu lu tr ng ph n mm Mi rosoft Ex el v tnh trung bnh I%


nng 10 g/ml, 50 g/ml, 100 g/ml ng Microsoft Excel.
Gi tr OD

iu in i ng M SD (M: gi tr OD trung bnh

tng mu, SD: l h hun , so s nh gi tr trung


hng ng test T-Student s

nh

ng ph n mm SPSS 18.0. S kh

mu th so vi
it

oi l

ngh thng k khi p < 0,05.


Tnh IC50 v khong tin y 95% IC50 ng phng ph p phn t h hi
quy pro it tr n ph n mm SPSS 18.0.

24

Ch g 3. KT QU V BN LUN
3.1. KT QU
3.1.1. K t qu s

g t

h x

thi oxi

se in vitro

iu, th vt
p dng phng ph p nh gi t

ng c ch xanthin oxidase in vitro trn

UV 96 ging Costar 3635 nh gi tc dng c ch XO in vitro ti 3 nng


100 g/ml 50 g/ml 10 g/ml ca 244 mu c liu, thc vt thu hi ti Vit
Nam.
3.1.1.1. Kt qu sng lc ca 47 mu dc liu c r sot thng tin v tc
dng
Kt qu sng l

147 mu liu th hin ng 3.1.

Nh xt:
-

Ti nng 100

g/ml, 116 mu th

th hin t

ng

(p < 0,05 , him 78,91% tng s mu th . Trong , 106 mu


r rt (p < 0,01 , him 72,12% tng s mu th ; 42 mu

h XO
h XO

h tr n 50%

hot tnh enzym, him 28,57% tng s mu th .


-

Ti nng 50 g/ml, 80 mu th

th hin t

ng

h XO

(p < 0,05 , him 54,42% tng s mu th . Trong , 69 mu t

h XO r rt (p < 0,01 , him 46,94% tng s mu th ; 23 mu

ng
h

tr n 50% hot tnh enzym, him 15,65% tng s mu th .


-

Ti nng 10 g/ml, 40 mu t
34 mu
enzym.

ng

h XO (p < 0,05 . Trong

h XO r rt (p < 0,01 ; 7 mu

h tr n 50% hot tnh

25

B g 3.1. nh hng ca cc mu dc liu ln hot XO in vitro ti cc nng 100 g/ml, 50 g/ml, 10 g/ml
N g 100 g/ml
STT

Tn Khoa hc

OD

I%

N g 50 g/ml
OD

N g 10 g/ml

I%

OD

I%

Afzelia xylocarpa Craib

0,152 0,024**

24,3

0,208 0,012**

13,3

0,207 0,008

2,0

Ageratum conyzoides L.

0,166 0,019**

23,5

0,179 0,013**

16,3

0,213 0,013

8,2

Alangium chinensis (Lour) Harms

0,098 0,025**

55,9

0,103 0,009**

51,7

0,141 0,013**

19,7

Alangium kurzii Craib

0,033 0,009**

84,3

0,058 0,005**

73,9

0,090 0,009**

48,4

Alocasia macrorrhizos (L) G. Don

0,191 0,014

11,7

0,204 0,015

5,0

0,215 0,012

7,4

Ancistrocladus wallichii Planch.

0,135 0,027**

38,8

0,173 0,019**

20,0

0,164 0,045**

23,6

Anoectochilus roxburghii (Wall.)

0,076 0,013**

61,9

0,133 0,008**

43,3

0,178 0,011**

15,9

Antidesma ambigumPax & Hoffm

0,025 0,015**

87,6

0,029 0,009**

87,3

0,044 0,007**

79,0

Antidesma montanum Bl

0,042 0,022**

79,3

0,055 0,024**

77,1

0,091 0,006**

57,1

10

Ardisia gigantifolia Stapf

0,150 0,014**

26,9

0,203 0,018

12,2

0,209 0,018

3,9

11

Ardisia gracillima

0,090 0,023**

58,6

0,168 0,012*

18,5

0,220 0,008

12

Area catechu L.

0,171 0,023**

22,9

0,170 0,015**

20,1

0,163 0,009

7,1

13

Artocarpus integrifolia (Thunb.) Merr.

0,158 0,017**

24,6

0,161 0,028

19,5

0,174 0,018

7,9

14

Asarum caudigerum Hance

0,197 0,006*

10,4

0,202 0,013

7,0

0,213 0,008

0,8

15

Baekea frutescens L.

0,084 0,030**

62,3

0,133 0,021**

37,4

0,144 0,009**

18,0

16

Balanophora sp

0,122 0,014**

38,5

0,186 0,017

17,9

0,207 0,011

1,0

17

Balanophora sp

0,128 0,019**

33,6

0,178 0,014**

21,1

0,187 0,010

8,5

18

Boehavia diffusa L.

0,217 0,016

2,1

0,226 0,016

0,169 0,007

3,2

19

Bohmerianivea (L.) Gaud.

0,030 0,023**

85,8

0,049 0,011**

79,1

0,106 0,013**

52,9

26

N g 100 g/ml
STT

Tn Khoa hc

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

0,171 0,023

11,1

0,208 0,019

8,0

0,200 0,009

2,4

0,007 0,003**

96,7

0,045 0,028**

77,7

0,083 0,007**

56,1

20

Bridelia balansae

21

Brucea javanica (L.)

22

Caesalpinia pulcherrima (L.) Sw

0,211 0,029

5,1

0,210 0,010

3,1

0,228 0,011

23

Callerya speciosa (Champ. Ex Benth.)Schot

0,259 0,029

0,253 0,055

0,175 0,008

24

Callicarpa heterotricha Merr.

0,179 0,016

10,5

0,222 0,005

7,6

0,214 0,016

25

Calophyllum inophyllum L.

0,166 0,014**

24,8

0,188 0,019

13,3

0,209 0,009

2,4

26

Calotropis gigantea (L.) Dryand ex Ait.f.

0,178 0,029**

17,8

0,176 0,011**

17,8

0,205 0,008

11,6

27

Campsis radicans (L.) Seem

0,154 0,013**

28,3

0,186 0,019*

13,3

0,174 0,015

5,5

28

Cancora petelotii

0,107 0,020**

46,4

0,169 0,011**

27,8

0,187 0,008**

11,7

29

Caryodaphnosis tonkinensisAiry-Shaw

0,123 0,026**

38,2

0,179 0,020**

21,0

0,191 0,011

8,8

30

Casytha filiformis L.

0,247 0,031

0,258 0,018

0,200 0,027

2,2

31

Cinnamomum iners Reinw.ex Blume

0,190 0,006**

13,7

0,196 0,009**

9,5

0,217 0,007

32

Cinnamomum iners Reinw.ex Blume

0,135 0,023**

35,2

0,190 0,018**

14,8

0,166 0,018

5,2

33

Cissus triloba (Lour.) Merr.

0,092 0,031**

58,0

0,140 0,031*

35,3

0,194 0,010

9,3

34

Clerodendrum cyrtophyllum Tucz

0,147 0,016**

26,3

0,199 0,013

12,6

0,214 0,009

35

Clerodendrum paniculatum L.

0,224 0,016

0,223 0,017

0,221 0,008

36

Coptis teeta wall

0,177 0,015

10,9

0,225 0,022

0,7

0,216 0,011

37

Cornus oligophlebia Merr.

0,116 0,018**

43,2

0,177 0,019**

22,1

0,191 0,018

10,9

38

Crotalaria assamica Benth

0,088 0,016**

57,1

0,145 0,018**

36,6

0,175 0,006**

18,2

39

Curculigo capitulata (Lour) Kuntze

0,153 0,013 **

28,7

0,167 0,014**

22,1

0,171 0,013

7,1

27

N g 100 g/ml
STT

Tn Khoa hc

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

40

Curculigo latifolia Dryand. Ex Ait.

0,184 0,008**

15,3

0,176 0,006

14,7

0,208 0,008

1,9

41

Cyclea fansipanensis Gagnep

0,190 0,022*

18,6

0,193 0,011

10,9

0,227 0,025

42

Dendrobium nobile Lindl

0,147 0,017**

31,6

0,179 0,017**

16,3

0,158 0,009**

14,3

43

Derris pachyloba

0,153 0,015**

25,1

0,199 0,014**

12,8

0,199 0,022

6,9

44

Desmodium rodgeri (Schindl.) Ohashi

0,184 0,016**

21,4

0,186 0,008*

14,0

0,215 0,028

2,1

45

Dichoroa febrifuga Lour.

0,040 0,019**

80,3

0,053 0,020**

76,9

0,080 0,008**

63,0

46

Dicliptera chinensis (L.) Nees

0,170 0,022**

19,0

0,196 0,034

2,0

0,178 0,019

5,9

47

Diptecarpus sp.

0,157 0,012**

21,7

0,222 0,012

5,2

0,208 0,011

1,9

48

Dracaena gracilis Wall. Ex Hook.f.

0,074 0,024**

62,9

0,106 0,012**

53,5

0,177 0,012**

17,3

49

Ehretia acuminate

0,118 0,016**

38,9

0,160 0,014**

29,1

0,182 0,017

11,2

50

Ehretia acuminate

0,189 0,015**

19,2

0,205 0,008

5,2

0,213 0,017

2,7

51

Eleaocarpus nitidus Jack

0,146 0,023**

26,7

0,210 0,014

7,5

0,201 0,010

4,2

52

Elsholtzia penduliflora W.W.Smith

0,102 0,012**

51,0

0,12 0,009**

46,8

0,178 0,012**

20,5

53

Equisetum diffusum D.Don

0,053 0,028**

75,6

0,072 0,022**

65,4

0,161 0,016**

24,1

54

Erythropalum scandens Blume

0,195 0,010*

10,3

0,203 0,022

1,7

0,220 0,008

55

Erythropalum scandens Blume

0,058 0,019**

80,1

0,088 0,011**

69,3

0,165 0,017**

43,0

56

Euria ciliata Merr L

0,070 0,018**

63,7

0,131 0,018**

42,2

0,176 0,028

14,1

57

Fallopia multiflora (Thunb.) Haraldson

0,056 0,013**

74,2

0,061 0,023**

71,4

0,200 0,038

13,9

58

Ficus elastica Roxb. Ex Horn

0,161 0,018**

27,5

0,176 0,011*

17,4

0,158 0,018

9,5

59

Flueggea virosa (Roxbex willd) Voigt

0,148 0,017**

22,8

0,183 0,022**

19,0

0,187 0,015

8,4

28

N g 100 g/ml
STT

Tn Khoa hc

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

0,149 0,022**

25,4

0,214 0,014

5,8

0,217 0,012

60

Glochidion eriocarpum Champ.

61

Glycosmis pentaphylla (Retz.) Correa

0,225 0,026

0,192 0,016

9,6

0,164 0,007

6,2

62

Hedyotis capitellata var mollis

0,220 0,017

1,0

0,222 0,010

0,167 0,012

4,4

63

Hedyotis capitellata var.mollis T.N.Ninh

0,100 0,032**

50,0

0,173 0,014**

26,2

0,189 0,012

10,6

64

Hedyotis hirsuta Spreng

0,203 0,016

2,4

0,239 0,017

0,225 0,017

65

Hedyotis ovata Thwaites ex Hook.f.

0,182 0,021

11,1

0,227 0,022

1,8

0,215 0,014

1,0

66

Helicteres hirsute Lour.

0,094 0,014**

53,0

0,155 0,012**

35,3

0,198 0,011

5,9

67

Helminthostachys zeylanica (L.) Hook

0,196 0,010

11,8

0,206 0,012

4,7

0,215 0,013

68

Hex godajam Colebr. Ex. Well.

0,164 0,016*

14,7

0,211 0,017

6,7

0,189 0,016

7,6

69

Hex godajamColebr. Ex.Well.

0,162 0,025

15,6

0,208 0,019

8,0

0,205 0,011

70

Hibiscus sagistifolius

0,065 0,024**

68,9

0,097 0,012**

58,1

0,156 0,013**

30,6

71

Hydnocarpus kurzii Warb.

0,140 0,025**

30,3

0,196 0,007**

18,5

0,191 0,009*

9,4

72

Hypericum patulum Ham. Ex. D.Don

0,159 0,015**

20,1

0,201 0,017

11,6

0,203 0,013

3,3

73

Hypericum uralum Thumb.

0,146 0,015**

28,5

0,212 0,017

7,0

0,205 0,020

4,3

74

Illigera parviflora Dunn

0,140 0,023**

30,0

0,206 0,016

9,1

0,211 0,015

1,5

75

Iodes cirrhosa Turcz.

0,185 0,015**

14,9

0,191 0,020

7,7

0,219 0,018

76

Iodes cirrhoza Turcz

0,127 0,017**

39,5

0,150 0,024*

25,0

0,178 0,024

5,7

77

Isotoma longifolia

0,180 0,027

10,0

0,215 0,011

10,6

0,210 0,012

0,4

78

Itoa orientalis

0,154 0,025**

24,8

0,205 0,026

9,9

0,207 0,012

3,4

79

Ixora coccinea L.

0,151 0,011**

30,2

0,169 0,016**

21,3

0,214 0,009*

8,0

29

N g 100 g/ml
STT

Tn Khoa hc

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

80

Ixora spp.

0,191 0,021

0,9

0,218 0,021

3,6

0,197 0,009

3,7

81

Jasminum subtriplinerve Blume

0,215 0,014

1,0

0,206 0,009

4,0

0,214 0,007*

7,7

82

Justicia gendarussa Burm .f.

0,209 0,007

5,3

0,217 0,013

0,0

0,211 0,010

1,8

83

Kaempferia rotunda L.

0,182 0,030**

15,2

0,197 0,010

8,2

0,182 0,009

0,8

84

Kalanchoe pinnnata (Lamk.) Pers.

0,189 0,019*

11,7

0,209 0,014

2,7

0,171 0,013

6,8

85

Knema globularia Warb.

0,162 0,024

18,9

0,234 0,008

2,6

0,214 0,008

86

Leea aequata L.

0,075 0,020**

62,8

0,113 0,012**

51,7

0,173 0,019*

18,1

87

Leucaena leucocephala (Lam.) De Wit

0,085 0,021**

61,7

0,093 0,007**

56,0

0,120 0,011**

30,0

88

Limacia scandes Lour.

0,192 0,019*

18,1

0,202 0,007

6,5

0,205 0,009

6,4

89

Lygodium scandens

0,110 0,021**

44,9

0,167 0,015**

26,5

0,206 0,010

3,5

90

Melastoma sanguineum Sims

0,186 0,020

6,6

0,234 0,013

0,226 0,011

91

Mesona chinensis Benth.

0,212 0,015

4,9

0,210 0,013

3,1

0,219 0,007

92

Microdesmis casearifolia Planch. Ex Hook

0,163 0,018**

18,2

0,199 0,019

12,4

0,200 0,008

4,5

93

Microdesmis casearifolia Planch. Ex Hook

0,192 0,019

3,5

0,218 0,018

4,1

0,206 0,013

1,8

94

Miliusa balansae Fin.& Gagnep.

0,056 0,021**

71,9

0,068 0,016**

71,7

0,094 0,016**

55,5

95

Mimusops elengi L.

0,119 0,016**

43,4

0,146 0,023**

27,2

0,164 0,017

13,4

96

Musra barjoo sieb.

0,132 0,029**

31,1

0,176 0,015**

22,3

0,193 0,018

5,7

97

Mussaenda cambodiana Pierre ex Pitard

0,040 0,011**

80,5

0,133 0,013**

42,4

0,201 0,010

7,4

98

Mussaenda cambodiana Pierre ex Pitard

0,197 0,016

9,5

0,204 0,011

1,3

0,204 0,011

4,0

99

Mussaenda densiflora Li

0,197 0,016

3,7

0,236 0,018

0,211 0,010

2,8

30

N g 100 g/ml
STT

Tn Khoa hc

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

100

Nepenthes mirabilis (Lour.) Druce

0,112 0,015**

49,7

0,138 0,010**

36,2

0,198 0,010

6,3

101

Nervilia fordii (Hance) Schlechter

0,148 0,010**

30,9

0,158 0,018**

26,3

0,149 0,012**

19,1

102

Oroxylon indicum

0,140 0,012**

31,6

0,184 0,014**

19,2

0,197 0,015

8,0

103

Osmanthus pedulculatus Gagnep.

0,075 0,020**

62,0

0,133 0,016**

41,5

0,188 0,015

10,1

104

Paederia consimilis Pierre ex Pitard

0,160 0,010**

23,1

0,214 0,016

7,8

0,214 0,019

4,6

105

Pandanus tinctorius

0,071 0,015**

68,3

0,09 0,007**

56,5

0,173 0,014**

18,1

106

Passiflora foetida L.

0,062 0,032**

70,3

0,083 0,010**

62,7

0,103 0,012**

41,2

107

Phyllanthus chamaepeuce Ridl

0,179 0,017*

13,9

0,232 0,009

0,0

0,216 0,017

3,5

108

Piper bavinumn C.DC.

0,090 0,010**

55,1

0,149 0,006**

36,2

0,174 0,012**

18,1

109

Piper sp

0,146 0,021**

28,7

0,190 0,019**

16,6

0,200 0,018

6,7

110

Pluchea indica (L.) Less

0,186 0,005**

14,3

0,186 0,011*

13,2

0,209 0,009**

9,8

111

Polygonum lanigerum R.Br.

0,093 0,024**

55,7

0,126 0,028**

37,0

0,183 0,017

3,1

112

Prunella vulgaris L.

0,100 0,015**

50,0

0,153 0,011**

34,6

0,182 0,016*

14,0

113

Pseuderanthemum bracteatum

0,179 0,017

12,2

0,203 0,023

12,1

0,201 0,015

7,4

114

Rauvolfia verticilata (Lour.) Bail

0,085 0,016**

63,9

0,107 0,016**

50,5

0,172 0,014**

21,6

115

Rhabdosia

0,084 0,023**

58,7

0,173 0,011**

24,0

0,200 0,019

6,5

116

Rhodomyrtus tomentosa (Ait.) Hassk

0,154 0,009**

29,9

0,174 0,011**

19,9

0,210 0,020

2,0

117

Rourea minor (Gaertn) Leenh.

0,201 0,016*

13,9

0,203 0,023

6,1

0,227 0,010

118

Rubus cochinchinensis Tratt.

0,204 0,009**

12,9

0,196 0,014

9,5

0,226 0,015

119

Rubus leucanthus Hance

0,186 0,013**

20,5

0,188 0,014

13,1

0,214 0,009

2,3

31

N g 100 g/ml
STT

Tn Khoa hc

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

120

Rumex chinensis Campd

0,126 0,018**

37,0

0,117 0,019**

36,3

0,183 0,013**

17,4

121

Sabia parviflora Wall.ex Rox

0,072 0,026**

64,9

0,102 0,013**

55,1

0,146 0,020**

32,0

122

Salacia cochinchinensis

0,201 0,023

0,234 0,016

0,206 0,013

123

Scoparia dulcis L.

0,144 0,018**

31,3

0,169 0,022

15,7

0,166 0,015

12,2

124

Selaginella uncinata (Desv.) Spring

0,054 0,030**

73,5

0,169 0,030**

27,1

0,202 0,018

7,1

125

Smilax corbularia Kunth

0,026 0,018 **

87,4

0,041 0,009**

82,1

0,097 0,005**

55,2

126

Smilax poilanei Gagnep.

0,055 0,013**

72,4

0,087 0,007**

61,7

0,119 0,008**

44,4

127

Solanum diphyllum L.

0,191 0,013

8,1

0,226 0,016

2,8

0,223 0,016

0,3

128

Solanum torvum Swartz.

0,075 0,010**

62,6

0,139 0,014**

42,1

0,175 0,011**

17,2

129

Stachytarpheta jamaicensis (L.) Vahl

0,214 0,011

3,9

0,217 0,008

0,1

0,217 0,016

130

Stachytarpheta jamaicensis (L.) Vahl

0,174 0,013**

20,8

0,195 0,014

10,2

0,206 0,011

4,0

131

Stephania longa Lour.

0,186 0,012**

20,4

0,201 0,019

7,1

0,211 0,019

3,7

132

Streptocaulon juventas (Lour.) Merr.

0,127 0,018**

38,0

0,202 0,015*

12,7

0,193 0,013*

11,1

133

Strobilanthes cystolithigera Lindau

0,190 0,011**

19,0

0,186 0,013*

14,2

0,212 0,021

3,4

134

Strobilanthes mucronatoproductus Lindau

0,195 0,011*

10,5

0,206 0,020

0,2

0,221 0,008

135

Styrax tonkinensis (Pierre) Craib ex Hartwiss

0,186 0,026**

13,2

0,202 0,011

5,6

0,174 0,009

5,2

136

Tabernaemontana bufalina (Lour)

0,176 0,036**

16,1

0,193 0,035

3,7

0,183 0,019

3,5

137

Tabernaemontana buffalina

0,178 0,021*

19,9

0,195 0,013

10,1

0,209 0,006

1,4

138

Tinospora crispa (L.) Miers.

0,189 0,010**

12,8

0,189 0,017

12,0

0,218 0,008

6,1

139

Uncaria acida (Hunt.) Roxb.

0,079 0,035**

62,2

0,101 0,025**

56,6

0,170 0,014**

24,3

32

N g 100 g/ml
STT

Tn Khoa hc

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

140

Uncaria cordata (Lour.) Merr

0,097 0,027**

51,3

0,150 0,013**

34,1

0,193 0,015

9,6

141

Uncaria macrophylla Wall. Ex Roxb.

0,151 0,013**

26,2

0,200 0,018*

12,1

0,202 0,006

5,7

142

Verbena officinalis L.

0,084 0,017**

60,9

0,113 0,011**

47,2

0,130 0,008**

29,4

143

Viburnum lutescens Bl.

0,154 0,014**

23,3

0,207 0,017*

14,0

0,201 0,012

4,7

144

Wikstroemia indica (L.) C.A Mey

0,062 0,025**

69,2

0,078 0,016**

57,6

0,173 0,008**

21,9

145

Wrightia dubia Spreng.

0,120 0,012**

39,9

0,172 0,016**

24,2

0,207 0,014

3,2

146

Wrightia laevis Hook.f.

0,155 0,020**

22,5

0,203 0,009**

13,5

0,205 0,014

3,0

147

Zizyphus mauritiana Lamk.

0,195 0,028

9,1

0,205 0,017

4,4

0,185 0,014

p < 0,05 so vi hng


p< 0,01 so vi hng

33

3.1.1.2. Kt qu sng lc 97 mu thc vt cha c thng tin v tc dng


Kt qu sng l

97 mu th vt th hin ng 3.2.

Nh xt:
-

Ti nng 100

g/ml, 70 mu th th hin t

ng

h XO

(p < 0,05 , him 72,16% tng s mu th . Trong , c 65 mu


XO r rt (p < 0,01 , him 67,01% tng s mu th ; 26 mu

h tr n

50% hot tnh enzym, him 26,80% tng s mu th .


-

Ti nng 50

g/ml, 50 mu th th hin t

ng

h XO

(p < 0,05 , him 51,55% tng s mu th . Trong , 45 mu t


ng
mu

h XO r rt (p < 0,01 , him 46,39% tng s mu th ; 11


h tr n 50% hot tnh enzym, him 11,34% tng s mu

th .
-

Ti nng 10 g/ml, 19 mu t
Trong , 11 mu

ng

h XO (p < 0,05 .

h XO r rt (p < 0,01 ; khng mu no

h tr n 50% hot tnh enzym.

34

B g 3. . nh hng ca cc mu thc vt ln hot XO in vitro ti cc nng 00 g/ml, 50 g/ml,


10 g/ml

STT

Mu

Tn khoa hc

3512

N g 100 g/ml

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

Aegiceras corniculatum

0,183 0,026*

18,1

0,219 0,021

2,3

0,219 0,011

3393

Albizia nigricans

0,052 0,012**

76,5

0,056 0,017**

74,0

0,122 0,021**

40,5

3340

Alchornea annamica

0,167 0,018**

27,1

0,189 0,020**

16,0

0,202 0,010

3,3

2993

Allamanda cathartica

0,077 0,019**

62,9

0,120 0,020**

42,8

0,194 0,008

8,1

3341

Allophylus cobbe

0,209 0,033

8,9

0,225 0,017

0,0

0,208 0,012

0,5

3023

Alpinia laosensis

0,166 0,011**

17,4

0,159 0,030

15,7

0,212 0,012

6,5

3403

Anisoptera costata

0,067 0,020**

71,0

0,112 0,020**

50,6

0,153 0,007**

26,0

2987

Antidesma montanum

0,225 0,015

0,198 0,024

5,6

0,235 0,020

3684

Archidendron lucidum

0,181 0,018**

21,1

0,212 0,018

5,8

0,214 0,010

10

2606

Artabotrys hienianus

0,161 0,007**

19,6

0,157 0,023

14,4

0,227 0,024

11

3088

Artocarpus asperulus

0,122 0,020**

42,0

0,118 0,011**

37,0

0,212 0,016

2,2

12

3324

Barringtonia pauciflora

0,146 0,023**

34,2

0,189 0,019

12,6

0,205 0,014

0,2

13

3097

Bauhinia cardinalis

0,109 0,011**

47,6

0,132 0,021**

37,1

0,210 0,007

0,3

14

3548

Bauhinia lakhonensis

0,088 0,010**

61,2

0,119 0,012**

46,7

0,183 0,010**

15,7

15

3312

Bauhinia purpurea

0,175 0,017**

21,1

0,212 0,023

2,1

0,204 0,021

0,6

16

3073

Bauhinia racemosa

0,223 0,030

0,1

0,233 0,021

0,212 0,009

17

2482

Biota onentalis

0,156 0,018*

22,4

0,155 0,029

15,3

0,216 0,010

2,6

35

STT

Mu

18

3368

19

Tn khoa hc

N g 100 g/ml

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

Bridelia retusa

0,071 0,016**

68,0

0,084 0,016**

61,2

0,136 0,011**

33,6

3757

Buchanania arborescens

0,076 0,018**

66,7

0,113 0,026**

49,7

0,153 0,018*

26,7

20

2891

Callicarpa arborea

0,101 0,008**

54,7

0,122 0,027**

45,8

0,174 0,013*

14,9

21

3773

Canarium subulatum

0,120 0,037**

47,7

0,138 0,013**

38,6

0,172 0,011*

17,7

22

3434

Canthium dicoccum

0,207 0,025

10,9

0,220 0,012

3,2

0,210 0,014

23

3635

Capparis annamensis

0,213 0,023

7,1

0,202 0,017

10,4

0,208 0,006

0,3

24

3428

Capparis beneolens

0,200 0,035

13,8

0,207 0,014

8,7

0,209 0,010

25

3086

Cissus adnata

0,158 0,017**

23,9

0,165 0,030

21,5

0,211 0,012

26

2948

Cleome viscosa

0,179 0,038

11,0

0,169 0,032

10,0

0,208 0,009

8,4

27

3977

Clerodendrum mandarinorum

0,170 0,012**

25,6

0,187 0,014**

16,5

0,202 0,008

6,9

28

3047

Colona auriculata

0,164 0,011**

21,2

0,177 0,030

15,7

0,211 0,014

29

2668

Coscinium fenestratum

0,200 0,010

5,0

0,178 0,029

5,1

0,216 0,012

0,5

30

3069

Croton caudatus

0,180 0,015

10,5

0,173 0,032

8,2

0,212 0,005

6,7

31

3075

Croton glandulosus

0,199 0,014

1,0

0,168 0,023

10,6

0,218 0,011

3,9

32

2790

Dacrycarpus imbricatus

0,133 0,020**

36,7

0,142 0,028*

24,3

0,201 0,009

7,4

33

3429

Dalbergia vietnamensis

0,163 0,028 **

29,7

0,206 0,017

9,3

0,024 0,008

1,5

34

2678

Decaspermum parviflorum

0,102 0,016**

51,5

0,120 0,035*

36,3

0,214 0,019

1,1

35

2507

Dillenia indica

0,065 0,020**

69,1

0,093 0,011**

58,4

0,118 0,019**

32,6

36

2745

Diospyros crumenata

0,095 0,016**

52,9

0,104 0,032**

43,3

0,197 0,011*

11,0

37

2793

Diospyros variegate

0,119 0,011**

43,4

0,121 0,029**

35,6

0,215 0,018

0,9

38

2744

Elaeocarpus grandiflorus

0,071 0,035**

66,2

0,095 0,025**

49,4

0,165 0,036*

24,1

36

STT

Mu

Tn khoa hc

39

3405

40

N g 100 g/ml

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

Elaeocarpus japonicas

0,112 0,012**

51,7

0,165 0,020**

27,1

0,189 0,009

8,8

3749

Elaeocarpus petiolatus

0,115 0,016**

49,7

0,149 0,011**

33,8

0,190 0,009

9,0

41

3064

Elaeocarpus stipularis

0,041 0,012**

79,8

0,041 0,020**

78,3

0,141 0,007**

38,0

42

2390

Equisetum diffusum

0,194 0,020

3,3

0,178 0,043

2,9

0,209 0,014

5,7

43

3370

Eriobotrya bengalensis

0,093 0,012**

57,9

0,134 0,015**

38,2

0,179 0,014

12,7

44

3700

Fernandoa brilletii

0,066 0,011**

71,4

0,097 0,015**

57,0

0,1530,005**

26,9

45

2868

Fissistigma oldhamii

0,181 0,022**

18,9

0,216 0,016

3,7

0,214 0,009

46

3381

Flueggea spirei

0,078 0,030**

64,7

0,157 0,032**

27,4

0,188 0,018

8,4

47

2657

Garcinia merguensis

0,183 0,018

13,0

0,175 0,038

6,7

0,215 0,008

0,7

48

3406

Glochidion littorale

0,040 0,020**

82,8

0,071 0,015**

68,8

0,106 0,009**

48,6

49

3353

Grewia hirsute

0,162 0,037**

29,3

0,198 0,030

11,9

0,190 0,016

9,0

50

3081

Grewia oligandra

0,203 0,021

2,1

0,190 0,022

9,5

0,225 0,012

51

2908

Helicia cochinchinensis

0,141 0,024**

36,8

0,162 0,009**

28,1

0,195 0,014

4,5

52

3666

Helicia nilagirica

0,215 0,022

6,4

0,236 0,020

0,217 0,009

53

2625

Hibiscus macrophyllus

0,187 0,018

10,2

0,200 0,017

10,3

0,162 0,015

7,5

54

3019

Hibiscus schizopetalus

0,155 0,013**

23,0

0,155 0,024

17,5

0,217 0,022

4,7

55

3076

Holarrhena crassifolia

0,206 0,034

7,8

0,214 0,027

4,6

0,224 0,004

56

3689

Homalium dasyanthum

0,113 0,023**

50,6

0,133 0,010**

40,8

0,183 0,007

12,4

57

3402

Hoya obovata

0,207 0,015*

10,7

0,218 0,019

4,1

0,209 0,014

58

2812

Jasminum longisepalum

0,140 0,013**

30,5

0,147 0,030

19,6

0,218 0,029

1,4

59

2766

Kadsura heteroclite

0,160 0,036

20,1

0,176 0,040

3,8

0,218 0,012

1,4

37

STT

Mu

60

3045

61

Tn khoa hc

N g 100 g/ml

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

Lagerstroemia crispa

0,112 0,016**

44,1

0,119 0,023**

36,8

0,207 0,015

9,0

2726

Lagerstroemia loudonii

0,083 0,032**

60,8

0,108 0,031**

42,3

0,203 0,009

6,2

62

2722

Lepisanthes rubiginosa

0,037 0,010 **

82,1

0,056 0,013**

75,0

0,117 0,032**

32,9

63

3778

Linociera ramiflora

0,070 0,008**

69,2

0,091 0,007**

59,4

0,150 0,006**

30,8

64

2936

Liquidambar formosana

0,173 0,012**

16,8

0,176 0,028

16,1

0,216 0,008

65

2562

Lithocarpus rhabdostachyus

0,112 0,023**

44,2

0,117 0,022**

35,9

0,189 0,010**

14,8

66

2953

Litsea glutinosa

0,135 0,019**

34,8

0,138 0,024**

34,4

0,199 0,010

5,5

67

3004

Lumnitzera racemosa

0,129 0,026**

38,5

0,123 0,018**

34,7

0,217 0,010

68

3439

Macaranga trigonostemonoides

0,127 0,022**

45,4

0,171 0,018**

24,8

0,190 0,009

8,2

69

2608

Macrosolen cochinchinensis

0,091 0,024**

56,5

0,121 0,008**

45,5

0,145 0,013**

17,0

70

3011

Malpighia glabra

0,193 0,009

3,9

0,176 0,033

6,5

0,216 0,009

5,0

71

3306

Merremia orientalis

0,159 0,020**

28,6

0,182 0,019*

16,0

0,190 0,012

7,1

72

2646

Metadina trichotoma

0,163 0,029*

21,7

0,189 0,009**

15,3

0,168 0,013

3,9

73

3322

Mitrephora thorelii

0,125 0,012**

43,9

0,179 0,022**

17,2

0,193 0,014

5,8

74

3052

Morus alba

0,129 0,017**

37,9

0,146 0,027*

30,4

0,202 0,009

4,0

75

3693

Mucuna biplicata

0,172 0,007**

24,7

0,196 0,009**

12,6

0,217 0,010

0,3

76

3334

Mussaenda frondosa

0,229 0,020

0,219 0,021

0,203 0,025

0,8

77

2841

Naravelia laurifolia

0,208 0,013

0,176 0,034

6,3

0,218 0,012

4,0

78

3459

Ormosia simplicifolia

0187 0,013**

18,0

0,190 0,017**

15,1

0,215 0,015

1,2

79

2884

Planchonella clemensii

0,167 0,025

16,7

0,164 0,039

12,9

0,223 0,010

1,7

80

3386

Polycarpaea corymbosa

0,152 0,012**

31,3

0,189 0,008**

12,6

0,210 0,021

38

STT

Mu

Tn khoa hc

81

3441

Prunus balansae

82

2764

83

N g 100 g/ml

N g 50 g/ml

N g 10 g/ml

OD

I%

OD

I%

OD

I%

0,229 0,024

1,3

0,230 0,010

0,222 0,011

Quercus franchetii

0,113 0,019**

43,7

0,134 0,033

26,6

0,216 0,006

2,6

2931

Ricinus communis

0,139 0,019**

37,9

0,181 0,018**

19,4

0,193 0,015

5,5

84

2921

Saraca dives

0,097 0,013 **

51,7

0,096 0,021**

48,9

0,212 0,017

6,8

85

3006

Sonneratia caseolaris

0,071 0,009**

65,9

0,074 0,020**

64,6

0,159 0,009**

24,5

86

3471

Styrax argentifolia

0,176 0,007**

22,6

0,186 0,017**

17,0

0,219 0,009

87

2915

Suregada multiflora

0,179 0,026*

19,9

0,208 0,027

7,3

0,198 0,012

3,4

88

2871

Tabernaemontana bovina

0,206 0,027

8,0

0,222 0,020

1,0

0,213 0,015

89

2733

Tarenna hoaensis

0,200 0,008

4,7

0,173 0,024

8,0

0,215 0,007

0,9

90

3421

Tephrosia purpurea

0,194 0,017**

16,3

0,204 0,015

10,0

0,203 0,012

2,0

91

3542

Terminalia corticosa

0,218 0,016

2,6

0,121 0,019

5,8

0,199 0,012

2,7

92

2971

Thespesia populnea

0,140 0,017**

32,6

0,141 0,021**

32,6

0,204 0,005

3,4

93

3663

Toona surenii

0,178 0,016**

20,3

0,211 0,018

6,2

0,219 0,010

94

3305

Toxocarpus villosus

0,144 0,006**

35,2

0,195 0,013

10,0

0,213 0,028

95

3423

Triumfetta bartramia

0,112 0,009**

51,6

0,155 0,016**

31,6

0,180 0,014

13,2

96

2801

Xantolis barauensis

0,053 0,013**

73,8

0,051 0,015**

72,2

0,112 0,022**

49,6

97

2763

Zanthoxylum scandens

0,190 0,015

9,5

0,180 0,030

4,1

0,225 0,010

* p<0,05 so vi mu chng
** p<0.01 so vi mu chng

39

3.1. . K t qu x

h IC50

u iu, th vt ti

S u khi nh gi s kh n ng c ch XO in vitro, x

nh c 68 mu

c tc dng c ch XO mnh nht (42 mu c liu v 26 mu thc vt) l cc


mu c tc dng c ch trn 50% hot tnh ca enzym nng 100 g/ml v c
kh n ng c ch r rt XO in vitro c 3 nng thc nghim (p < 0,01). Cc mu
ny c thit lp dy nng kho s t x

nh IC50.

Allopurinol mt thuc c ch mnh XO c in vitro v in vivo, c tc dng


lm h acid uric huyt th nh tr n lm sng c s dng lm cht i chiu trong
th nghim.
Kt qu IC50 ca cc mu c liu, thc vt tim n ng c trnh by
bng 3.3.
B g 3.3. Nng c ch 50% hot tnh enzym xanthin oxidase (IC50) ca cc mu
dc liu, thc vt tim nng
STT
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25

Tn ph thng/ M
Allopurinol
Qu ng
Qu ng lng
3393
2993
3403
Kim tuyn Sapa
Chi mi m
Chi mi gn lm
Sang trng
Chi xu
3548
Gai
3368
S u u t chut
3757
2891
Cha vi
Lc lc l i di
2678
Th g s
2507
2745
Pht d mnh
2744
3405

Tn khoa hc
Alangium chinensis (Lour) Harms
Alangium kurzii Craib
Albizia nigricans Gagnep.
Allamanda cathartica L.
Anisoptera costata Korth.
Anoectochilus roxburghii (Wall.)
Antidesma ambigum Pax & Hoffm
Antidesma montanum Bl.
Ardisia gracillima
Baekea frutescens L.
Bauhinia lakhonensis Gagnep.
Bohmeria nivea (L.) Gaud
Bridelia retusa (L.) Spreng.
Brucea javanica (L.)
Buchanania arborescens Blume
Callicarpa arborea Roxb.
Cissus triloba (Lour.)
Crotalaria assamica Benth
Decaspermum parviflorum (Lamk.) J.Scott.
Dichoroa febrifuga Lour.
Dillenia indica L.
Diospyros crumenata
Dracaena gracilis Wall. Ex Hook.f.
Elaeocarpus grandiflorus Smith
Elaeocarpus stipularis Sieb

IC50 (g/ml)
0,15 (0,14-0.17)
65,00 (50,76-90,06)
13,01 (8,03-17,76)
18,01 (12,19-23,64)
71,21 (59,55-85,60)
36,35 (28,82-45,44)
69,13 (57,41-85,21)
3,30 (2,70-3,85)
8,76 (7,80-10,20)
98,17 (81,75-123,57)
66,22 (33,11-202,88)
53,289 (32,55-80,17)
10,60 (7,92-27,46)
32,50 (23,82-42,80)
10,83 (8,07-24,40)
43,78 (32,08-56,80)
78,34 (61,82-102,76)
80,89 (54,57-144,91)
94,90 (63,97-183,26)
100,64 (66,48-207,85)
8,58 (7,90-9,48)
33,87 (26,01-43,19)
77,18 (63,62-95,34)
50,15 (40,97-62,92)
48,11 (36,16-61,82)
85,93 (44,34-269,45)

40

STT
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68

Tn ph thng/ M
3064
Kinh gii r
C thp bt
3370
Dy hng
Sm lng
H th
3700
3381
3406
D cm
T kn
Bo sm
3689
2726
Gi hc bng
2722
Keo du
3778
2608
Mi liu
Bm bc campuchia
V san l di
Da di
Lc tin
Tiu ba v
Ngh trng
H kh tho
Ba gc
Mt gu
Hoa khoe cy nho
2921
Quyn b
Kim cang
Kim cang poilane
C di hoa trng
3006
3423
Cu ng
Cu ng l hnh tim
C roi nga
Nit gi
2801

Tn khoa hc
Elaeocarpus japonicas Blume
Elsholtzia penduliflora W.W. Smith
Equisetum diffusum D.Don
Eriobotrya bengalensis (Roxb.) Hook. F.
Erythropalum scandens Blume
Euria ciliata Merr L
Fallopia multiflora (Thunb.) Haraldson
Fernandoa brilletii (Dop)Steen.
Flueggea spirei Beille
Glochidion littorale Blume
Hedyotis capitellata var. mollis T.N.Ninh
Helicteres hirsuta Lour.
Hibiscus sagistifolius
Homalium dasyanthum (Turcz.) Warb.
Lagerstroemia loudonii Teysm. & Binn.
Leea aequata L.
Lepisanthes rubiginosa (Roxb.) Leenh.
Leucaena leucocephala (Lam.) De Wit
Linociera ramiflora (Roxb.) Wall. Ex G.
Macrosolen cochinchinensis (Lour.)Tiegh.
Miliusa balansae Fin.Gagnep.
Mussaenda cambodiana Pierre ex Pitard
Osmanthus pedulculatus Gagnep.
Pandanus tinctorius
Passiflora foetida L.
Piper bavinumn C.DC.
Polygonum lanigerum R.Br.
Prunella vulgaris L.
Rauvolfia verticilata (Lour.) Bail
Rhabdosia
Sabia parviflora Wall.ex Rox
Saraca dives Pierre
Selaginella uncinata (Desv.) Spring
Smilax corbularia Kunth
Smilax poilanei Gagnep.
Solanum torvum Swartz.
Sonneratia caseolaris (L.) Engl.
Triumfetta bartramia L.
Uncaria acida (Hunt.) Roxb.
Uncaria cordata (Lour.) Merr.
Verbena officinalis L.
Wikstroemia indica (L.) C.A Mey
Xantolis barauensis (Lecomte) P. Royen
( ???) Khng x

nh c

IC50 (g/ml)
16,23 (???)
83,47 (64,82-115,89)
30,86 (24,94-37,36)
91,30 (73,18-119,69)
18,43 (12,43-24,23)
61,94 (52,99-73,05)
34,28 (21,34-51,12)
46,27 (29,22-44,65)
79,98 (37,39-219,77)
14,28 (8,84-19,46)
121,36 (108,10-139,77)
94,53 (78,88-118,10)
31,05 (23,23-39,95)
97,26 (76,39-132,80)
74,18 (62,48-88,66)
50,68 (41,43-63,62)
18,32 (13,65-22,88)
43,66 (32,80-59,68)
25,42 (17,33-33,89)
68,87 (54,33-88,64)
9,40 (7,02-18,38)
51,61 (41,35-63,68)
73,62 (63,08-87,43)
49,27 (40,97-60,32)
25,52 (17,15-34,34)
85,70 (69,39-111,36)
84,77 (74,25-98,39)
123,00 (93,70-199,24)
53,08 (43,11-67,58)
94,49 (69,39-148,20)
39,98 (30,45-42,82)
83,43 (47,14-200,02)
75,43 (47,08-147,66)
8,57 (6,83-22,65)
20,00 (11,71-28,06)
77,91 (46,87-179,94)
34,35 (???)
91,49 (74,16-117,97)
46,26 (36,80-59,48)
110,29 (89,09-156,18)
52,39 (25,86-114,43)
44,60 (36,26-55,58)
12,52 (5,18-19,49)

41

Nhn xt: Cc mu c liu, thc vt c la chn u th hin tc dng


c ch XO kh tt, t ng theo nng . Kt qu x
sng l

nh IC50 ph hp vi kt qu

n u. Trong , cc mu th c tc dng c ch mnh XO th hin

gi tr IC50 thp bao gm:


Chi mi m (Antidesma ambigum Pax & Hoffm) vi IC50 = 3,30 g/ml.
Kim cang (Smilax corbularia Kunth) vi IC50 = 8,57 g/ml.
Thng sn (Dichoroa febrifuga Lour.) vi IC50 = 8,58 g/ml.
Chi mi gn lm (Antidesma montanum Bl.) vi IC50 = 8,76 g/ml.
Mi liu (Miliusa balansae Fin. & Gagnep) vi IC50 = 9,40 g/ml.
Gai (Bohmeria nivea (L.) Gaud) vi IC50 = 10,60 g/ml.
S u u t chut (Brucea javanica L.) vi IC50 = 10,83 g/ml.
Xantolis barauensis (Lecomte) P. Royen vi IC50 = 12,52 g/ml.

3.2. BN LUN
3.2.1. V k t qu s

3.2.1.1. V i tng a vo sng lc


Vi cc mu dc liu c r sot thng tin v tc dng trc khi sng lc
N m 2013, nhm nghin cu ti B mn Dc lc Trng i h Dc
H Ni tin hnh nghin cu tip cn sng lc da trn kinh nghim s dng cc
c liu trong dn nhn vi tc dng chng vim, chng oxy h v iu tr cc
bnh v xng khp. Tuy nhin, kt qu thu c h

o v h t m r c

nhiu cy thuc c tim n ng c ch mnh XO so vi cc nghin cu trn th gii.


Ti nng 100 g/ml, c 74,73% mu th hin tc dng c ch enzym, 9,89%
mu c ch trn 50% hot tnh enzym; trong M n a (Archidendron clyearia) l
mu c kh n ng c ch xanthin oxidase mnh nht vi IC50 = 15,6 g/ml [11].
Trong khi

nghi n u trn th gii cho kt qu khong 20% n 50% cc

mu c ch trn 50% hot tnh ca enzym nng 100 g/ml [21], [56], [59],
[71], [91], [110], [116]. Hn na, nghin cu sng lc tr n UV 96 ging Costar
3596 ca Tr n Ngc Yn Chi cho thy loi c tc dng c ch mnh enzym xanthin
oxidase (Asarum balansae, Microdesmis casearifolia, Antidesma ambigum) li l

42

loi m h

thng tin v vic s dng trong dn gi n ng nh h

nghin cu trn th gii v tc dng chng vim, chng oxy ha, h

i uri nhng

cc loi khc trong hi c nghin cu nhiu v cc tc dng ny [5]. V


vy, khng b st cc cy c tc dng tt, c bit l cc loi phn b trng
Vit N m h c nghin cu, ti tin hnh r sot thng tin v cc tc
dng trn khng ch trong cc ti liu y v n,

o iu tra tri thc v kinh

nghim s dng trong nhn dn m cn m rng r sot, tm kim thng tin trn c
cc nghin cu c cng b v loi v v chi ca loi .
147 mu c liu la chn vo sng lc l

c liu c ghi nhn

mt trong cc tc dng chng vim, chng oxy ha, h acid uric, c ch XO, cha
bnh xng khp v cc triu chng lin quan hoc thuc cc chi thc vt c
nghin cu v cc tc dng ny.
Vi cc mu thc vt cha c thng tin v tc dng trc khi a vo sng
lc
Phng ph p nh gi t

ng c ch xanthin oxidase in vitro tr n UV

96 ging Costar 3635 c trin khai cho php tin hnh vi c mu ln, tit
kim thi gian v chi ph nghin cu so vi phng ph p o qu ng truyn thng
tr y, to iu kin thun li v n gin ha cc nghin cu sng l . Hn
na, vic r sot thng tin tn km kh nhiu thi gian, i khi th b b st
thng tin. Cc thc vt ( h phi c liu thng h c nghin cu nhiu
v thng tin cn hn ch. Chnh v nhng l do trn, ti m rng i tng sng
lc trn c cc mu thc vt h

thng tin v vic tc dng, cng dng nhm

tm kim, pht hin thm cc loi c tc dng c ch xanthin oxidase tt t ngun


thc vt h c nghin cu nc ta. ng thi, thng qua kt qu sng lc
ny c th so snh c t l cc mu c tim n ng gi 2 hng tip cn sng lc
c r sot thng tin v tc dng v h

thng tin. T , c th c nhng

nhn nh v nh hng cho cc nghin cu sng lc tip theo.


Mi mu c th 3 nng 100 g/ml, 50 g/ml, 10 g/ml. y ng l
cc mc nng thng c cc nghin cu thc hin [21], [71], [85], [116]. Mi

43

th nghim u c lp li 3 l n/ngy, trong 3 ngy khc nhau m bo tnh


chnh xc v lp li ca kt qu.
3.2.1.2. V kt qu sng lc
Cc nghin cu trn th gii theo hng tip cn sng lc da trn kinh
nghim s dng c liu trong dn gian hoc nn y hc c truyn cho thy kt qu
khong 20-50% cc mu c ch trn 50% hot tnh ca enzym nng 100 g/ml
[56], [59], [71], [91], [116]. Cng theo hng tip cn sng lc ny, nghin cu ca
tc gi Nguyn Th Thanh Mai vi 288 dch chit t 96 c liu thu hi ti 4 tnh
min Nam Vit Nam cho thy 46 dch chit ( khong 15,97%) c s c ch ln hn
50% hot tnh enzym nng 100 g/ml [85]. Nghin cu trn 91 mu c liu
c s dng theo kinh nghim, thu hi ti cc tnh min Bc v min Trung Vit
Nam ca nhm nghin cu ti B mn Dc lc, Trng i h Dc H Ni cho
thy kt qu c 9 mu (khong 9,89%) c ch trn 50% hot tnh enzym nng
100 g/ml [11]. Nh vy, cc nghin cu sng lc Vit Nam c t l c liu
tim n ng thp hn so vi cc nghin cu trn th gii.
ti ny, tip cn sng l theo h i hng t cc ngun mu khc nhau.
Kt qu thu kh kh qu n khi so s nh vi
nghi n u trong n v s lng

nghi n u tr n th gii v

mu tim n ng

hng tip cn sng lc th nht,

h x nthin oxi se.

n u c 187 mu c liu c r

sot thng tin. T thng tin tra cu c v tc dng chng vim, chng oxy ha,
c ch XO, h acid uric trong cc ti liu y v n,

o iu tra tri thc v kinh

nghim s dng trong dn gian (m rng r sot thng tin trn c chi ca c liu)
la chn c 147 mu vo sng lc.
Kt qu tng i kh quan, pht hin c nhiu c liu c tc dng c
ch XO mnh. nng 100g/ml, c 42 mu (28,57%) c ch trn 50% hot tnh
enzym. nng 10 g/ml, c 7 mu (4,67%) c ch trn 50% hot tnh ca
enzym. Vic m rng r sot thng tin trn c hi c liu c th gip trnh b st
cc loi c tim n ng tt m h c nghin cu v ghi nhn tc dng (c bit l

44

nhng loi ch phn b trng nc ta), trong khi th gii nhiu nghin
cu v hi c liu ny.
hng tip cn sng lc th hai, bao gm 97 mu thc vt h

thng

tin v tc dng sinh hc.


Nghin cu sng lc p dng phng ph p o qu ng tr n UV 96 ging
vi h thng ELISA to iu kin thun li cho vic tm kim, pht hin v nh
gi tc dng h acid uric in vitro t ngun ti nguy n c liu, thc vt phong ph
c nc ta.
Kt qu thu c rt kh quan, c 70 mu (72,16%) th hin tc dng c ch
enzym, 26 mu (26,80%) c ch trn 50% nng 100/ml. nng thp hn
50 g/ml, 10g/ml t l s mu th hin tc dng c ch, t l s mu c ch trn
50% hot tnh enzym thp hn so vi hng tip cn sng l u tin. nng
50 g/ml, c 11 mu (11,34%) c ch ln hn 50% hot tnh ca enzym. nng
10 g/ml, khng cn mu no c kh n ng c ch trn 50% hot tnh XO.
Kt qu ny cng khng nh nc ta c tim n ng rt ln v c liu ni
hung v

c liu c tc dng h acid uric ni ring. Nhiu loi h c ghi

nhn v tc dng trong cc ti liu, y v n ng nh h

nghi n u v tc

dng sinh hc lin qu n n iu tr bnh gt nhng ho thy kh n ng c ch XO


kh tt trn th nghim in vitro.
Cc mu c liu, thc vt c tc dng c ch mnh XO (68 mu c liu,
thc vt c la chn tip t x
b ri rc cc h kh

nh IC50. Cc loi c tc dng tt ny phn

nh u, trong 4 h c nhiu loi c tc dng l: H C ph

- Rubiaceae (6 loi), h Th u d u - Euphorbiaceae (5 loi) v h u - Fabaceae (4


loi), h Hoa mi - Lamiaceae (3 loi).
Trong cc mu vo sng lc, c mt s mu cng chi hay cng loi
nhng phn dng kh nh u, ni thu hi khc nhau c th so snh kh n ng
c ch XO gia cc mu , t t m r mu c tc dng tt hn. kt qu sng
lc, nhng c liu cng chi hoc cng loi cho kt qu kh tng ng, nhiu
loi thuc cng mt chi c tim n ng tt nh Chi mi m (IC50 = 3,30 g/ml) v

45

Chi mi gn lm (IC50 = 8,76 g/ml), Kim cang (IC50 = 8,57 g/ml) v Kim cang
poilante (IC50 = 20,00 g/ml), Cu ng (IC50=46,26 g/ml) v Cu ng l hnh
tim (IC50 = 110,29 g/ml).
3.2.1.3. Mt s lu khi p dng phng php nh gi tc dng c ch XO in
vitro sng lc
Vic p dng phng ph p nh gi t

ng c ch xanthin oxidase in vitro

tr n UV 96 ging Costar 3635 c trin khai ti Trng i h Dc H


Ni mang li nhiu u im vt tri cho nghin cu sng lc: tit kim thi gian,
chi ph nghin cu, chnh x

v lp li cao.

Trong qu trnh nghin cu, c n lu n mt s vn c th gp phi nh


hng n kt qu nghin cu.
+ Cc yu t nh hng n hot tnh ca enzym mc 1.2.3. u lm nh hng
n t v m phn ng gy sai s cho kt qu o quang. V vy c n kim
sot cht ch cc yu t ny gim thiu ti

s i s.

Nhit : Yu t ny khng ch nh hng n t phn ng, m cn nh


hng n hot tnh enzym, t

ng n kt qu o. Mc d enzym c 15 pht

nhit 250C v tin hnh phn ng nhit 250C nhng thc t nhn thy vn
c s

o ng t 5 n 10% v kt qu gi iu kin thi tit m ng v m

h. V vy, gim thiu sai s do nhit


m iu nhit Memmert 250C v khi l

th o t

u c tin hnh trong t

vi my l kh y ng i t

nhit 250C m bo iu kin tin hnh th nghim lun l 250C. Xanthin


oxi se c bo qun 40C m bo hot tnh, c pha long v hot 0,01
U/ml ng y trc khi tin hnh phn ng v lun bo qun trong iu kin lnh
(n

n khi cho vo tng ging.

Dung dch c cht xanthin, dung dch th pha ngay trong ngy lm th nghim.
C ht x nthin tan thp (1g/14,5L 160C, 1g/1,4L 1000C t n t ng theo
nhit . V vy, phi ha tan nng xanthin trn my khuy t khi bt u ngy lm
th nghim m bo xanthin ha tan ht t nng 150M. Mu c liu,
thc vt c pha thnh dung dch g 10 mg/ml trong DMSO bo qun. T

46

dung dch gc pha long thnh cc nng thch hp trong m phosphat pH 7,5
trong ngy lm th nghim. Vi ph long nh vy m bo ha tan, bo qun
c cc mu cao c

c liu, thc vt v nng DMSO trong ging nh hn

1% khng lm nh hng n hot tnh ca enzym [85].


+ Phn ng oxy ha xanthin c n s tham gia ca oxy khng kh, v vy m bo
phn ng xy ra ti c n tr nh y np h y lm kn

, tr nh trong

my lc khay trong khong thi gian 30 pht phn ng.


3.2.2. V k t qu

h x

thi oxi

se

thu

ti

3.2.2.1. V kt qu xc nh IC50
Theo cc tc gi, c liu c IC50 i 100 g/ml c coi l c tim n ng
[9], [21], [59], [71], [85], [91], [110], [116]. V vy, nhm nghin cu ng a trn
s ny la chn mu vo x

nh IC50.. ti x

nh IC50 ca 68

mu th c kh n ng c ch xanthin oxidase mnh (cc mu c ch trn 50% hot


tnh ca enzym nng 100 g/ml v tc dng c ch th hin r rt c 3 nng
kho st 100 g/ml, 50 g/ml, 10 g/ml).
Cc c liu tim n ng nh gi thng s IC50 bng phng ph p
nh gi t

ng c ch XO in vitro. Cc loi c IC50 thp, cho thy kh n ng c

ch XO mnh l Chi mi m (Antidesma ambigum) vi IC50 = 3,30 g/ml, Kim


cang (Smilax corbularia) vi IC50 = 8,57 g/ml, Thng sn (Dichoroa febrifuga)
vi IC50 = 8,58 g/ml, Chi mi gn lm (Antidesma montanum) vi IC50 = 8,76
g/ml, Mi liu (Miliusa balansae) vi IC50 = 9,40 g/ml, Gai (Bohmeria nivea)
vi IC50 = 10,60 g/ml, S u u t chut (Brucea javanica) vi IC50 = 10,83
g/ml, Xantolis barauensis vi IC50 = 12,52 g/ml. Mc d IC50 c
ny

c liu

o hn (22-82 l n) so vi thu i chiu allopurinol ( IC50= 0,154 g/ml)

nhng kt qu ny vn rt kh quan. Mu c s dng trong nghin cu l cao


ton ph n c

c liu, khng phi l cht tinh khit nh llopurinol n n kh n ng

c ch km hn so vi allopurinol. Trong cc nghin cu ng ,


c IC50 i 100 g/ml c coi l c tim n ng v IC50 c

c liu

c liu c tim n ng

47

ng thng cao gp 60-600 l n so vi allopurinol [9], [21], [56], [59], [85], [91],
[110], [116].
3.2.2.2. V cc mu tim nng nht
C

c liu ny u c IC50 thp hn so vi y M n a (IC50=15,6

g/ml) l c liu c tc dng c ch XO mnh nht trong nghin cu tr


ca cng nhm nghin cu [11].
Kt qu thu c c s ph hp v c mi lin quan vi kinh nghim s
dng

c liu ny trong thc t v cc nghin cu trn th gii c cng

b v loi, chi ca

c liu ny.

S u u t chut (Brucea javanica) cn c tn gi khc l S u u rng,


Xoan rng, Kh sm nam l cy thu c s dng trong nhn n cha l,
vim rut, st trng vt thng [13]. Trn th gii nhiu nghin cu v c
liu ny: Brucea javanica c chng minh c tc dng chng oxy ha lin quan
n cc hp cht T nin c phn lp [18]; chng vim in vitro [30], [35], [122];
tc dng khng t bo ung th c nghin cu rng ri [50],[73], [121].
Gai (Bohmeria nivea) c s dng theo kinh nghim tr vi m ng tit
niu, li tiu, vim t cung, phong thp t bi [6], [13]. Cy G i c chng
minh c tc dng chng vim, bo v gan [76], [109]; chng oxy ha [75].
Mi liu (Miliusa balansae): trong dn gian s

dng cy Mi liu lng

Milius velutina vi cng dng tiu vim, st trng, c m mu [6], [13]. Hp cht
Chrysosplenol C ( 4,5,6 trihydroxy -3,3,7- trimethoxyflavone ) phn lp t cy
Miliusa balansae c chng minh c tc dng chng oxy ha, khng t bo ung
th [62], [107].
Thng sn (Dichoroa febrifuga) l mt v thu c s dng lu i
trong ng y cha bnh st rt [13]. G n y, Thng Sn c chng minh
c tc dng chng vim rt tt c in vitro v in vivo [32], [94].
Kim cang (Smilax corbularia) l cy thu c s dng trong dn gi n
tr phong thp, gn xng u nhc, cha d ng v c tc dng chng vim [6],

48

[13]. Trn th gii ng nghin cu v tc dng chng vim c

c liu ny

[101].
H i c liu Chi mi m (Antidesma ambigum) v Chi mi gn lm
(Antidesma montanum) thuc cng chi Antidesma (Chi Chi mi hay Chi mi) u
th hin tc dng c ch XO rt tt, trong y Chi mi m th hin tc dng c
ch XO mnh nht vi IC50 = 3,30 g/ml g n vi IC50 ca allopurinol (IC50 = 0,15
g/ml). Chi Antidesma thuc h Th u d u (Euphorbiaceae), c ngun gc t vng
ng N m vi khong 100 loi, phn b cc vng nhit i v cn nhit i
hu , hu ,

o thuc Thi B nh Dng v hu Phi [7], [57]. Thi

Lan v Malaysia c khong 13 loi [57]. Vit Nam c 26 loi, thuc 2 phn chi, 6
nh nh, trong khong 6 loi c hu mc hoang vng rng th v i khi
vng ng bng [7]. L mt chi c s phong ph

ng v loi,

thnh ph n

ha hc vi cu trc ha h c bit, c hot tnh sinh hc v cng dng dn gian


qu bu [7]. Theo ng y s dng cc b phn ca cy Chi mi vi cng dng
khc nhau: v cy cha vim rut, a chy; qu cha ho, vim phi; l cha nga
da, l u; ho cha t thp [17]. M

o kho hc no v 2 loi

Chi mi m (Antidesma ambigum ) v Chi mi gn lm (Antidesma montanum)


phn b nc ta nhng

hp cht polyphenolic cng tc dng chng oxy ha,

chng nhim khun, khng t bo ung th c bo co vi mt s cy trong chi


Antidesma. Antidesma thwaitesianum (Chi mi l dai) c chng minh c kh
n ng hng oxy h vt tri vi cc hp cht phenolic, chng nhim khun [37],
[98]; Antidesma madagascariense ng c nghin cu kh n ng hng oxy
ha [78]; Antidesma acidum (Chi mi chua) c ghi nhn tc dng chng ung th
do cc hp cht phenolic c trong cy [66]. Nghin cu ha thc vt t qu
Antidesma bunius (Chi mi t

phn lp c cc loi flavonoid khc nhau, cc

hp cht phenolic v chng minh c tc dng chng oxy ha ca mt s hp cht


ny [26], [102]. Nh vy hot ng c ch xanthin oxidase ca Antidesma ambigum
v Antidesma montanum ng th do s hin din ca cc thnh ph n phenolic
v fl vonoi tng t nh

y kh

trong hi Antidesma.

49

Xantolis barauensis l loi cy ly g, hin nay h thy c bo co v


thnh ph n ha h

ng nh

C 8 c liu tr n h

tc dng ca n.

nghi n u no v tc dng h

i uri

ng

nh c ch enzym x nthin oxi se c cng b c trong n v ngoi nc. T


kt qu sng l , ti ng gp ph n chng minh s

ng sinh hc Vit Nam,

vi vic pht hin nhiu loi c tim n ng c ch XO in vitro m h tng c


nghin cu. V vy, y l nhng ng gp mi c ti.

50

KT LUN
Trong khun kh ti "Sng lc cc cy thuc Vit Nam c tc dng c
ch xanthin oxidase in vitro", hng ti thu c nhng kt qu sau nh s u:
1. sng lc tc dng c ch xanthin oxidase in vitro ca 244 mu thc
vt, c liu. Trong :
- Ti nng 100 g/ml, c 186 mu th th hin tc dng c ch XO
(p < 0,05), chim 76,2% tng s mu th . Trong , c 171 mu c ch XO r rt
(p < 0,01), chim 70,1% tng s mu th ; 68 mu c ch trn 50% hot tnh enzym.
- Ti nng 50 g/ml, c 130 mu th

th hin tc dng c ch XO

(p < 0,05 , him 53,3% tng s mu th . Trong , c 114 mu c tc dng c ch


XO r rt (p < 0,01 , him 46,7% tng s mu th ; 34 mu c ch trn 50% hot
tnh enzym.
- Ti nng 10 g/ml, c 59 mu c tc dng c ch XO (p < 0,05 . Trong
, 45 mu
- x
2. x

h XO r rt (p < 0,01 ; 7 mu c ch trn 50% hot tnh enzym.


nh c 68 mu tim n ng.
nh c IC50 ca 68 mu tim n ng. La chn c 8 mu c

kh n ng c ch XO mnh nht l:
Antidesma ambigum (IC50 = 3,30 g/ml), Smilax corbularia (IC50 = 8,57
g/ml), Dichoroa febrifuga (IC50 = 8,58 g/ml), Antidesma montanum (IC50 = 8,76
g/ml), Miliusa balansae (IC50 = 9,40 g/ml), Bohmeria nivea (IC50 = 10,60 g/ml),
Brucea javanica (IC50 = 10,83g/ml), Xantolis barauensis (IC50 = 12,52 g/ml).
Trong , Antidesma ambigum l mu c kh n ng c ch XO vt tri so vi cc
mu khc vi IC50 l 3,30 g/mg.

51

XUT
ti sng lc c tc dng c ch XO in vitro ca 244 mu thc vt,
c liu v t m r c Antidesma ambigum (Chi mi m), Smilax corbularia
(Kim cang), Dichoroa febrifuga (Thng sn , Antidesma montanum (Chi mi
gn lm), Miliusa balansae (Mi liu), Bohmeria nivea (Gai), Brucea javanica (S u
u t chut) , Xantolis barauensis l cc loi c kh n ng c ch XO in vitro tt
nht. Tuy nhi n, c th khai thc, s dng hiu qu hn ngun c liu Vit
N m trong vi phng, iu tr nh gt v

nh kh

li n qu n n t ng

uri , ti xin xut mt s ni dung nghin cu tip theo:


-

M rng quy m sng l tip tc t m kim cc liu c tim n ng


c ch XO ti Vit Nam.

Phn on dch chit, phn lp cht v x

nh hot tnh v h c

ch xanthin oxidase in vitro t cc loi c tc dng c ch XO mnh


nht: Antidesma ambigum, Smilax corbularia, Dichoroa febrifuga,
Antidesma montanum, Miliusa balansae, Bohmeria nivea, Brucea
javanica, Xantolis barauensis.
-

nh gi t

ng h acid uric, cc tc dng li n qu n n bnh gt

(gim u, hng vim, chng oxy ha) in vivo v th c tnh c

liu: Antidesma ambigum, Smilax corbularia, Dichoroa febrifuga,


Antidesma montanum, Miliusa balansae, Bohmeria nivea, Brucea
javanica, Xantolis barauensis ph t trin

c liu tim n ng ny

trong iu tr ri lon chuyn h li n qu n n t ng

i uri m u.

TI LIU THAM KHO


TING VIT
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Hong Qunh Hoa, Phm Thanh K, Phng L n (2009 , "T


v gan c

ng bo

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tn thng g n ng paracetamol chut nht ", Tp ch Y hc thc hnh.


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PH LC
Ph lc 1. Thng tin v cc mu dc liu a vo sng lc
STT

Tn ph thng

Tn Khoa hc

Ni thu

H KH

BP dng

Tc dng

G
1

Afzelia xylocarpa Craib.

Fabaceae

Ba V, H Ni

Ht

Kit l, vim gan [13]

Ng sc

Ageratum conyzoides L.

Asteraceae

H Giang

CCTT

St trng [13]

Qu ng

Alangium chinensis (Lour)

Alangiaceae

H Giang

CCTT

Chng oxy ha [130]

Qu ng lng

Alangium kurzii Craib

Alangiaceae

Tn K, Ngh An

CCTT

Chng vim [13]

Ry

Alocasia macrorrhizos (L) G. Don

Araceae

H Giang

CCTT

Mn nht, gh l [13]

Trung qun

Ancistrocladus wallichii Planch.

Ancistrocladaceae

i T, Thi Nguyn

CCTT

Cha st virus [45]

Kim tuyn Sapa

Anoectochilus roxburghii (Wall.)

Orchidaceae

Sa Pa, Lo Cai

Ton cy

Chng oxy ha [33]

Chi mi m

Antidesma ambigumPax & Hoffm

Euphorbiaceae

Ba V, H Ni

Cnh non, l

Chi: chng oxh [37], [98]

Chi mi gn lm

Antidesma montanum Bl.

Euphorbiaceae

Ba V, H Ni

thn, l

Chi: chng oxh [37], [98]

10

L khi

Ardisia gigantifolia Stapf

Myrsinaceae

VQG Bch M

Chng ung th [77]

11

Sang trng

Ardisia gracillima

Myrsinaceae

Phong Nha-K Bng

Trn mt t

12

Cau

Area catechu L.

Arecaceae

H Nam

CCTT

Cha vim rut [13]

13

Mt t n

Artocarpus integrifolia Merr.

Moraceae

CCTT

CCTT

Ch sng v [13]

14

Th t tn

Asarum caudigerum Hance

Aristolochiaceae

Sa Pa, Lo Cai

CCTT

Cha vim hng [13]

15

Chi xu

Baekea frutescens L.

Myrtaceae

Ngh An

CCTT

Tr u nhc [13]

16

C gi t

Balanophora sp

Balanophoraceae

Ba V, H Ni

Cnh,l

Chng oxy ha [120]

Ardisia tinctoria: chng nhim


khun [68]

STT

Tn ph thng

Tn Khoa hc

H KH

Ni thu

BP dng

Tc dng

17

C gi t

Balanophora sp

Balan phoraceae

Ba V, H Ni

Trn mt t

Chng oxy ha [120]

18

Sm t

Boehavia diffusa L.

Nyctaginaceae

Hng Ha, Qung Tr

CCTT

Vim gan [13]

19

Gai

Bohmeria nivea (L.) Gaud.

Urticaceae

VQG Bch M

R: Chng vim [75]

20

Thu mt

Bridelia balansae

Euphorbiaceae

Ba V, H Ni

Thn, l

Vim hng [6]

21

S u u t chut

Brucea javanica (L.)

Simaroubaceae

Qunh Lu, Ngh An

CCTT

St trng, l [13]

22

Kim phng

Caesalpinia pulcherrima (L.) Sw

CCTT

Vn Kiu, Qung Tr

CCTT

Cha si thn [16]

23

Ct sm

Fabaceae

H Ni

CCTT

24

T chu lng

Callicarpa heterotricha Merr.

Verbenaceae

Ba V, H Ni

Cnh, l

Vim da [13]

25

M u

Calophyllum inophyllum L.

Clusiaceae

TP H Ch Minh

CCTT

Thp khp, l lot [13]

26

Bng bng l hen

Calotropis gigantea (L.) Dryand

Asclepiadaceae

Chng M , H Ni

CCTT

Vi m ng h hp [13]

27

ng ti u

Campsis radicans (L.) Seem

Bignoniaceae

Hng Y n

CCTT

Chng oxh [34]

28

Cy xuyn l

Cancora petelotii

Gentiliaceae

Sa Pa, Lo Cai

Ton cy

Chng vim [13]

29

Ca l bc

Caryodaphnosis tonkinensis

Lauraceae

Ba V, H Ni

Cnh, l

Cha vim khp [13]

30

T x nh

Casytha filiformis L.

Lauraceae

Ba V, H Ni

ton cy

Chng oxy ha [6]

31

Qu rng

Cinnamomum iners Reinw.ex Blume

Lauraceae

Tn Lc, Ha Bnh

CCTT

Phong thp [13]

32

D hng

Cinnamomum iners

Lauraceae

Tnh min ni pha Bc

CCTT

Vim rut, l [13]

33

Cha vi

Cissus triloba (Lour.) Merr.

Vitaceae

Duy Tin, H Nam

CCTT

Cha thp khp [13]

34

B my

Clerodendrum cyrtophyllum Tucz

Verbenaceae

Vn quc gia Bch M

Trn mt t

S pht ban [13]

35

M ho

Clerodendrum paniculatum L.

Verbenaceae

Phong Nha-K Bng

Cnh, l

Vim gan [13]

Callerya speciosa (Champ.ex Benth.)


Schot

Thp khp, u lng, vi m g n


mn tnh [13]

STT

Tn ph thng

Tn Khoa hc

H KH

Ni thu

BP dng

Tc dng

36

Hong lin

Coptis teeta wall

Ranumculaceae

Ba V, H Ni

Thnr

Khng khun [13]

37

Gic mc t gn

Cornus oligophlebia Merr.

Cornaceae

Ba V, H Ni

Cnh, l

T [13]

38

Lc lc l i di

Crotalaria assamica Benth

Fabaceae

Ba V, H Ni

Cnh, l

R: Chng vim [70]

39

C n ho u

Curculigo capitulata (Lour) Kuntze

Amaryllidaceae

T m o, Vnh Ph

CCTT

Phong thp [13]

40

Sm cau l rng

Curculigo latifolia Dryand. ex Ait.

Hypoxidaceae

Qung Bnh

Thn r

T [13]

41

Dy sm ni cao

Cyclea fansipanensisGagnep

Menispermaceae

Ba V, H Ni

Thn r

Cha d ng da [13]

42

Thch hc

Dendrobium nobile Lindl

Orchidaceae

Hng Y n

CCTT

u khp [13]

43

Duc c

Derris pachyloba

Fabaceae

Ba V, H Ni

Thn, l

L, vim rut [13]

44

Thc lp

Desmodium rodgeri (Schindl.)

Fabaceae

Phong Nha-K Bng

Cnh, l

Tr giun [13]

45

Thng sn

Dichoroa febrifuga Lour.

Hydrangeaceae

VQG Bch M

R: Chng vim [32]

46

L in

Dicliptera chinensis (L.) Nees

Acanthaceae

CCTT

Bo v gan [126]

47

Tai th

Diptecarpus sp.

Dipterocarpaceae

Sa Pa, Lo Cai

Qu

Chng vim [123]

48

Pht d mnh

Dracaena gracilis Wall. ex Hook.f.

Dracaenaceae

VQG Bch M

Phong thp [13]

49

Cm rng

Ehretia acuminata

Boraginaceae

Ba V, H Ni

Cnh, l

Bo v gan [10]

50

Cm rng

Ehretia acuminata

Boraginaceae

Phong Nha-K Bng

Cnh, l

Bo v gan [10]

51

Cm lng

Eleaocarpus nitidus Jack

Eleaocarpaceae

Ba V, H Ni

Chng ung th [93]

52

Kinh gii r

Elsholtzia penduliflora W.W. Smith

Lamiaceae

VQG Bch M

Trn mt t

Vim yt h u [13]

53

C thp bt

Equisetum diffusum D.Don

Equisetaceae

Qung Bnh

Trn mt t

Vim tit niu [13]

54

B khai

Erythropalum scandens Blume

Erythropalaceae

Qung Bnh

C cy

Chng vim [13]

55

Dy hng

Erythropalum scandens Blume

CCTT

Vn Kiu, Qung Tr

CCTT

Chng vim [16]

STT

Tn ph thng

Tn Khoa hc

H KH

Ni thu

BP dng

Tc dng

56

Sm lng

Euria ciliata Merr L

Theaceae

Ba V, H Ni

Cnh, l

Chng lo ha [111]

57

H th

Fallopia multiflora Haraldson

Polygonaceae

Qun B, H Giang

CCTT

L, nga [13]

58

Bp

Ficus elastica Roxb. Ex Horn

Moraceae

Ngh An

CCTT

Cha vim rut [13]

59

N qu trng

Flueggea virosa

Euphorbiaceae

Ba V, H Ni

Chng co git [95]

60

Bt ch lng

Glochidion eriocarpum

Euphorbiaceae

VQG Bch M

cnh, l

Ph thn [13]

61

Bi bung

Glycosmis pentaphylla (Retz.) Correa Rutaceae

Tin Phc, Qung Nam

CCTT

Mn nht [13]

62

D cm

Rubiaceae

Bc Quang, H Giang

CCTT

63

D cm

Rubiaceae

Sa Pa, Lo Cai

64

An in lng

Hedyotis hirsuta Spreng.

Rubiaceae

VQG Bch M

C cy

Thp khp [13]

65

An in trng

Hedyotis ovata Thwaites ex Hook.f.

Rubiaceae

VQG Bch M

Cnh, l

Chng ung th [88]

66

T kn ci

Helicteres hirsuta Lour.

Sterculiaceae

Ba V, H Ni

iu tr ung th [31]

67

Sm bng bong

Helminthostachys zeylanica Hook

CCTT

Vn Kiu, Qung Tr

CCTT

Cha phong thp [16]

68

Rt

Hex godajam Colebr. ex. Well.

Hicaceae.

Ba V, H Ni

a chy, li tiu[13]

69

Rt

Hex godajamColebr. ex. Well.

Hicaceae

Ba V, H Ni

a chy, li tiu [13]

70

Bo sm

Hibiscus sagistifolius

Malvaceae

VQG Bch M

Thn r

Bo v d dy [13]

71

L ni

Hydnocarpus kurzii Warb.

Flacourtiaceae

Ba V, H Ni

Trn mt t

Phong hi, l lot [13]

72

Ban trn

Hypericum patulum Ham. Ex. D.Don

Hypericaceae

Ba V, H Ni

Cnh

Cha ung th [119]

73

Ban l nh

Hypericum uralum Thumb.

Hypericaceae

Ba V, H Ni

Cnh non, l

Tr sng u [13]

Hedyotis capitellata var mollis


(Pierre ex Pitard)
Hedyotis capitellata var. mollis
T.N.Ninh

Ph n trn
mt t

Cha lot d dy, vi m li v


hng [13]
Chng oxy ha [19]

STT

Tn ph thng

Tn Khoa hc

H KH

Ni thu

BP dng

Tc dng

74

Li n ng

Illigera parviflora Dunn

Hernandiaceae

VQG Bch M

CCTT

Cy tm D o [13]

75

C r y xanh

Iodes cirrhosa Turcz.

Menispermaceae

Qung Bnh

Trn mt t

Chng vim [48]

76

Mc thng

Iodes cirrhoza Turcz

Icacinaceae

Qun B, H Giang

CCTT

Ung th v [99]

77

M mt

Isotoma longifolia

Lobeliaceae

Ba V, H Ni

Trn mt t

Hen, kh th [13]

78

Cm

Itoa orientalis

Flacourtiaceae

Ba V, H Ni

Chng nm [112]

79

Trang mu n

Ixora coccinea L.

Rubiaceae

Tnh min ni pha Bc

CCTT

Ch u nhc [13]

80

Khm p

Ixora spp.

Rubiaceae

Ba V, H Ni

Cnh, l

Khng khun [72]

81

Ch vng

Jasminum subtriplinerve Blume

Blume Oleaceae

H Ni

CCTT

p-xe v,u nht [13]

82

Thanh to

Justicia gendarussa Burm .f.

Acanthaceae

T m o, Vnh Ph

CCTT

T thp [13]

83

a lin

Kaempferia rotunda L.

Zingiberaceae

Tnh min ni pha Bc

CCTT

Cha t thp [13]

84

Trng sinh

Kalanchoe pinnnata (Lamk.) Pers.

Crassulaceae

Hng Y n

CCTT

Ch sng u [13]

85

Mu ch l nh

Knema globularia Warb.

Myristicaceae

Ba V, H Ni

ht

Gh, st trng [13]

86

Gi hc bng

Leea aequata L.

Leeaceae

Sa Pa, Lo Cai

Thp khp [13]

87

Keo du

Leucaena leucocephala (Lam.) Wit

Mimosaceae

H Giang

CCTT

Chng oxy ha [55]

88

Kng

Limacia scandes Lour.

Menispermaceae

Qung Bnh

Thn, l

Chng tr m cm [69]

89

Bng bong

Lygodium scandens

Lygodiaceae

VQG Bch M

Dy mang l

St trng, l lot [13]

90

Mua b

Melastoma sanguineum Sims

Melastomataceae

VQG Bch M

Cnh, l

Qu: chng oxh [47]

91

Th h en

Mesona chinensis Benth.

Lamiaceae

Tnh min ni pha Bc

CCTT

Cha vim khp [13]

92

Chn

Pandaceae

Ba V, H Ni

Thn, l

Microdesmis casearifolia Planch. ex


Hook

Chng oxy ha- bo v t bo


gan [8]

STT

Tn ph thng

93

Chn

94

Mi liu

95
96
97

98

99

Tn Khoa hc
Microdesmis casearifolia Planch. ex

Ni thu

H KH

BP dng

Tc dng
Chng oxy ha- bo v t bo

Pandaceae

Ba V, H Ni

Thn, l

Miliusa balansae Fin. & Gagnep.

Annonaceae

Ba V, H Ni

Cnh, l

Chng ung th [62]

Sn ct

Mimusops elengi L.

Sapotaceae

Ti n Phc, Qung Nam

CCTT

Chng oxy ha [105]

Chui ht

Musra barjoo sieb.

Musaceae

Ba V, H Ni

Qu xanh

Chng oxy ha [127]

Bm bc

Mussaenda cambodiana Pierre ex

campuchia

Pitard

Rubiaceae

VQG Bch M

Cnh, l hoa

Hoa: Li tiu [13]

Rubiaceae

Phong Nha-K Bng

Thn, l

Hoa: Li tiu [13]

Mussaenda densiflora Li

Rubiaceae

VQG Bch M

cnh l.

T thp, u nhc [13]

Bm bc
Bm bc hoa
d y

Hook

Mussaenda cambodiana Pierre ex


Pitard

gan [8]

100

Np m

Nepenthes mirabilis (Lour.) Druce

CCTT

Vn Kiu, Qung Tr

CCTT

Si tit niu [16]

101

Lan 1 l

Nervilia fordii (Hance) Schlechter

Orchidaceae

Sn L

CCTT

Mn nht [13]

102

Nc nc

Oroxylon indicum

Bignoniaceae

Ba V, H Ni

V thn

Chng d ng [13]

103

V san l di

Osmanthus pedulculatus Gagnep.

Oleaceae

Ba V, H Ni

Cnh, l

104

Thi t

Paederia consimilis Pierre ex Pitard

Rubiaceae

VQG Bch M

Cnh l

L, vim rut [13]

105

Da di

Pandanus tinctorius

Pandaceae

Phong Nha-K Bng

Thn

Gim m mu [129]

106

Lc tin

Passiflora foetida L.

Passifloraceae

Kon Tum

CCTT

Chng vim [104]

107

Cu tri

Phyllanthus chamaepeuce Ridl.

Euphorbiaceae

VQG Bch M

ton cy

Chng vim [13]

Chi Osmanthus: chng


oxh,chng NK [60], [61]

STT

Tn ph thng

Tn Khoa hc

H KH

Ni thu

BP dng

Tc dng

108

Tiu ba v

Piper bavinumn C.DC.

Piperaceae

Ba V, H Ni

Trn mt t

Vim rut [13]

109

Tiu rng

Piper sp

Piperaceae

Ba V, H Ni

Mt t

Chng oxy ha [6]

110

Cc t n

Pluchea indica (L.) Less

Asteraceae

Cao Bng

CCTT

Ch u lng [13]

111

Ngh trng

Polygonum lanigerumR.Br.

Polygonaceae

Kho i Chu, Hng Y n

CCTT

Cha rn cn [13]

112

H kh tho

Prunella vulgaris L.

Lamiaceae

Sa Pa, Lo Cai

Cm hoa

H HA [13]

113

Xun hoa

Pseuderanthemum bracteatum

Acanthaceae

VQG Bch M

Vim rut [13]

114

Ba gc

Rauvolfia verticilata (Lour.) Bail

Apocynaceae

Phong Nha-K Bng

H HA [13]

115

Mt gu

Rhabdosia

Lamiaceae

Ba V, H Ni

116

Sim

Rhodomyrtus tomentosa (Ait.) Hassk

Myrtaceae

Bc Cn

CCTT

Chng vim [13]

117

Ngng chan hy

Rourea minor (Gaertn) Leenh.

Connaraceae.

Phong Nha-K Bng

Cnh non, l

Chng st rt [13]

118

Ngy hng

Rubus cochinchinensis Tratt.

Rosaceae

Phong Nha-K Bng

Thn

Vim gan [13]

Rubus leucanthus Hance

Rosaceae

Phong Nha-K Bng

Cnh, l

Rumex chinensis Campd

Polygonaceae

M Chu, Sn L

CCTT

Mn nht [13]

Sabia parviflora Wall.ex Rox

Sabiaceae

Ba V, H Ni

Tr sng ty [13]

119
120
121

m m ho
trng
Cht cht
Hoa khoe cy nho
gc

Vim gan B [28]

u g n, vng

, k h th h

tiu ha [13]

122

Chc mu

Salacia cochinchinensis

Celastraceae

Ba V, H Ni

Thn r

Tr T [13]

123

Cam tho t

Scoparia dulcis L.

Scrophulariaceae

VQG Xun Sn

CCTT

Vim hng [13]

124

Quyn b

Selaginella uncinata (Desv.) Spring

Selaginellaceae

Vn quc gia Bch M

Ton cy

Thiu oxy huyt [131]

125

Kim cang

Smilax corbularia Kunth

Smilacaceae

VQG Bch M

Thn, r

Chng vim [101]

STT

Tn ph thng

Tn Khoa hc

H KH

Ni thu

BP dng

Tc dng

126

Kim cang poilane

Smilax poilanei Gagnep.

Smilacaceae

VQG Bch M

Thn r

Phong thp [13]

127

C 2 l

Solanum diphyllum L.

Solanaceae

VQG Bch M

Ton cy

Chng oxh [43]

128

C di hoa trng

Solanum torvum Swartz.

Solanaceae

Ba V, H Ni

Thn, l

Tr nt n [13]

129

ui hut

Stachytarpheta jamaicensis (L.)Vahl

CCTT

Vn Kiu, Qung Tr

CCTT

Vim tit niu [16]

130

ui hut

Stachytarpheta jamaicensis (L.)Vahl

Verbenaceae

Kon Tum

CCTT

Chng vim [108]

131

Li tin

Stephania longa Lour.

Menispermaceae

Phong Nha-K Bng

Dy thn, l

Chng vim [125]

132

H th trng

Streptocaulon juventas (Lour.) Merr.

Asclepidaceae

VQG Bch M

Thn r

Chng ung th [114]

133

Chm rng

Strobilanthes cystolithigera Lindau

Acanthaceae

Ba V, H Ni

Cnh, l

Chng vim [13]

134

Chm ta

Acanthaceae

Phong Nha-K Bng

Cnh, l

Chng vim [13]

135

Styracaceae

Hm Yn, Tuyn Quang

CCTT

Cha vim ph qun [13]

136

Li tru choi

Tabernaemontana bufalina (Lour)

Thi Nguyn

CCTT

137

Li tru choi

Tabernaemontana buffalina

Apocynaceae

Phong Nha-K Bng

Trn mt t

138

Dy k ninh

Tinospora crispa .Miers.

Menispermaceae

L Sn, H B nh

CCTT

Cha l lot [13]

139

Cu ng

Uncaria acida (Hunt.) Roxb.

Rubiaceae

VQG Bch M

Cnh c mc

Cao HA [13]

Uncaria cordata (Lour.) Merr.

Rubiaceae

VQG Bch M

Thn c mc

Cao HA [13]

140

Cu ng l hnh
tim

Strobilanthes mucronatoproductus
Lindau
Styrax tonkinensis (Pierre) Craib ex
Hartwiss

Apocynaceae

Chi: Chng oxh, chng vim


[24], [64]
Chi: Chng oxh, chng vim
[24], [64]

STT

Tn ph thng

Tn Khoa hc

H KH

Ni thu

BP dng

Tc dng

141

Cu ng l ln

Uncaria macrophylla Wall. ex Roxb.

Rubiaceae

Ba V, H Ni

Cnh, l

H HA [13]

142

C roi nga

Verbena officinalis L.

Verbanaceae

Qun B, Bc Giang

CCTT

L ng , u khp [13]

143

Vt vng nht

Viburnum lutescens Bl.

Caprifoliaceae

Ba V, H Ni

Trn mt t

144

Nit gi

Wikstroemia indica (L.) C.A Mey

Thymelaeaceae

Ngh An

CCTT

Mn nht [13]

145

Lng mc ng

Wrightia dubia Spreng.

Apocynaceae

VQG Bch M

Chng ung th [91]

146

Mc chm

Wrightia laevis Hook.f.

Appocynaceae

Sa Pa, Lo Cai

Cnh, l

147

To di

Zizyphus mauritiana Lamk.

Rhamnaceae

Hng Y n

CCTT

Cy Viburnum tinus: chng


oxy ha [124]

Cy lng mc cng chi: chng


vim [81]
Cha l nga [13]

Ph lc 2. Thng tin cc mu thc vt a vo sng lc

H khoa hc

Ni thu

Tn khoa hc

STT

Mu

B phn dng

3512

Myrsinaceae

Aegiceras corniculatum (L.) Blanco

VQG Ct B

L + cnh nh

3393

Fabaceae

Albizia nigricans Gagnep.

Hm Thun, Bnh Thun

L + cnh nh

3340

Euphorbiaceae

Alchornea annamica Gagnep.

B Gia Mp, B nh Phc

L + cnh nh

2993

Apocynaceae

Allamanda cathartica L.

Ph Hng, C i Nc, C Mau

L + dy

3341

Sapindaceae

Allophylus cobbe (L.) Raeusch.

B Gia Mp, B nh Phc

L + cnh nh

3023

Zingiberaceae

Alpinia laosensis Gagnep.

Sc Tr ng

Ton cy

3403

Dipterocarpaceae

Anisoptera costata Korth.

Hm Thun Nam, Bnh Thun

L + cnh nh

2987

Euphorbiaceae

Antidesma montanum Blume

Khnh Bnh, Tr n V n Thi, C Mau

L + cnh nh

3684

Fabaceae

Archidendron lucidum (Benth.) I. Nielsen

Dak Som, Dak Glong, Dak Nong

L + cnh nh

10

2606

Annonaceae

Artabotrys hienianus Ban

S Sn, S Th y, Kon Tum

L + dy

11

3088

Moraceae

Artocarpus asperulus (Gagnep.) Jarr.

Tn Bnh, La Gi, Bnh Thun

L + cnh nh

12

3324

Lecythidaceae

Barringtonia pauciflora King

B Gia Mp, B nh Phc

L + cnh nh

13

3097

Fabaceae

Bauhinia cardinalis Pierre ex Gagnep.

Tn Ha, Tn Thnh, B Ra- Vng Tu

L + dy

14

3548

Caesalpiniaceae

Bauhinia lakhonensis Gagnep.

Ea Sol, Ea H'leo, Dak Lak

L + thn

15

3312

Caesalpiniaceae

Bauhinia purpurea L.

B Nho, Ph Long, B nh Phc

L + cnh nh

16

3073

Fabaceae

Bauhinia racemosa Lamk.

Hm Thun Nam, Bnh Thun

L + cnh nh

17

2482

Cupressaceae

Biota onentalis (L.) Ende.

Ha Ninh, Ha Vang, Nng

L + cnh nh

H khoa hc

Ni thu

Tn khoa hc

STT

Mu

B phn dng

18

3368

Euphorbiaceae

Bridelia retusa (L.) Spreng.

VQG C t Ti n, Tn Ph, ng Nai

L + cnh nh

19

3757

Anacardiaceae

Buchanania arborescens (Blume) Blume

VQG Ph Quc, Kin Giang

L + cnh nh

20

2891

Verbenaceae

Callicarpa arborea Roxb.

Yn L, Nh Xun, Thanh Ha

L + cnh nh

21

3773

Burseraceae

Canarium subulatum Guillaum.

VQG Cn o, B Ra-Vng Tu

L + cnh nh

22

3434

Rubiaceae

Canthium dicoccum (Gaertn.) Teysm. & Binn.

Vnh Hi, Ninh Hi, Ninh Thun

L + cnh nh

23

3635

Capparaceae

Capparis annamensis (Baker f.) Jacobs

Vnh Tn, Tuy Phong, B nh Thun

L + thn

24

3428

Capparaceae

Capparis beneolens Gagnep.

Vnh Hi, Ninh Hi, Ninh Thun

L + thn

25

3086

Vitaceae

Cissus adnata Roxb.

Tn Tin, La Gi, Bnh Thun

L + dy

26

2948

Capparaceae

Cleome viscosa L.

T m Dng, Vnh Ph

Ton cy

27

3977

Verbenaceae

Clerodendrum mandarinorum Diels

L Dng, Lm ng

L + thn

28

3047

Tiliaceae

Colona auriculata (Desf.) Craib

Ph Sn, Tn Ph, ng Nai

L + cnh nh

29

2668

Menispermaceae

Coscinium fenestratum (Gaertn.) Colebr.

A Yun, Mang Yang, Gia Lai

L + thn

30

3069

Euphorbiaceae

Croton caudatus Geiseler

Gia Huynh, Tnh Linh, Bnh Thun

L + cnh nh

31

3075

Euphorbiaceae

Croton glandulosus L.

Tn Thun, Hm Thun Nam, Bnh Thun

L + cnh nh

32

2790

Podocarpaceae

Dacrycarpus imbricatus Blume

Da Chais, L Dng, Lm ng

L + cnh nh

33

3429

Fabaceae

Dalbergia vietnamensis Phamh.

Vnh Hi, Ninh Hi, Ninh Thun

L + cnh nh

34

2678

Myrtaceae

Decaspermum parviflorum (Lamk.) J. Scott.

Ho Sn, Krong Bong, k L k

L + cnh nh

35

2507

Dilleniaceae

Dillenia indica L.

H Ninh, H V ng, Nng

L + cnh nh

36

2745

Ebenaceae

Diospyros crumenata Thwaites

E So, E k r, k L k

L + cnh nh

37

2793

Ebenaceae

Diospyros variegata Kurz

Da Chais, L Dng, Lm ng

L + cnh nh

Ni thu

Tn khoa hc

STT

Mu

H khoa hc

B phn dng

38

2744

Elaeocarpaceae

Elaeocarpus grandiflorus Smith

Dak Lak prov., Eakar distr., Ea So comm.

L + cnh nh

39

3405

Elaeocarpaceae

Elaeocarpus japonicus Sieb. & Zucc.

Hm Thun Nam, Bnh Thun

L + cnh nh

40

3749

Elaeocarpaceae

Elaeocarpus petiolatus (Jack) Wall.

VQG Ph Quc, Kin Giang

L + cnh nh

41

3064

Elaeocarpaceae

Elaeocarpus stipularis Blume

c Thun, Tnh Linh, Bnh Thun

L + cnh nh

42

2390

Equisetaceae

Equisetum diffusum D. Don

Lim Ph, V n Bn, Lo C i

Ton cy

43

3370

Rosaceae

Eriobotrya bengalensis (Roxb.) Hook. f.

VQG C t Ti n, Tn Ph, ng Nai

L + cnh nh

44

3700

Bignoliaceae

Fernandoa brilletii (Dop) Steen.

Bng Ring, Xuyn Mc, B Ra-Vng T u

L + cnh nh

45

2868

Annonaceae

Fissistigma oldhamii (Hemsl.) Merr.

VQG V Qu ng, H Tnh

L + cnh nh

46

3381

Euphorbiaceae

Flueggea spirei Beille

VQG C t Ti n, Tn Ph, ng Nai

L + cnh nh

47

2657

Clusiaceae

Garcinia merguensis Wight

A Yun, Mang Yang, Gia Lai

L + cnh nh

48

3406

Euphorbiaceae

Glochidion littorale Blume

Hm Thun Nam, Bnh Thun

L + cnh nh

49

3353

Tiliaceae

Grewia hirsuta Vahl

c Liu, B ng, B nh Phc

L + cnh nh

50

3081

Tiliaceae

Grewia oligandra Pierre

Tn Thun, Hm Thun N m, B nh Phc

L + cnh nh

51

2908

Proteaceae

Helicia cochinchinensis Lour.

Hi Vn, Nh Th nh, Th nh H

L + cnh nh

52

3666

Proteaceae

Helicia nilagirica Bedd

inh Tr ng Thng, Di Linh, Lm ng

L + cnh nh

53

2625

Malvaceae

Hibiscus macrophyllus Roxb. ex Hornem.

S Sn, S Th y, Kom Tum

L + cnh nh

54

3019

Malvaceae

Hibiscus schizopetalus (Mast.) Hook. f.

Thanh Qu, Th nh Tr , S Tr ng

L + cnh nh

55

3076

Apocynaceae

Holarrhena crassifolia Pierre ex Spire

Tn Thun, Hm Thun Nam, Bnh Thun

L + cnh nh

56

3689

Flacourtiaceae

Homalium dasyanthum (Turcz.) Warb.

Bo L , Lm ng

L + cnh nh

57

3402

Aslepiadaceae

Hoya obovata Decne.

Hm Thun Nam, Bnh Thun

L + thn

H khoa hc

Ni thu

Tn khoa hc

STT

Mu

B phn dng

58

2812

Oleaceae

Jasminum longisepalum Merr.

Vnh Hi, Ninh Hi, Ninh Thun

L + dy

59

2766

Schisandraceae

Kadsura heteroclita (Roxb.) Craib

L Dng, Lm ng

L + cnh nh

60

3045

Lythraceae

Lagerstroemia crispa Pierre ex Laness.

Tn Ph, ng Nai

L + cnh nh

61

2726

Lythraceae

Lagerstroemia loudonii Teysm. & Binn.

E So, E k r, k L k

L + cnh nh

62

2722

Sapindaceae

Lepisanthes rubiginosa (Roxb.) Leenh.

E So, E k r, k L k

L + cnh nh

63

3778

Oleaceae

Linociera ramiflora (Roxb.) Wall. ex G. Don

VQG Cn o, B Ra-Vng Tu

L + cnh nh

64

2936

Altingiaceae

Liquidambar formosana Hance

Ngc Thnh, Phc Y n, Vnh Ph

L + cnh nh

65

2562

Fagaceae

Lithocarpus rhabdostachyus A. Camus

D k M n, k Glei, Kon Tum

L + cnh nh

66

2953

Lauraceae

Litsea glutinosa (Lour.) C. B. Robins.

T m o, Vnh Ph

L + cnh nh

67

3004

Combretaceae

Lumnitzera racemosa Willd.

Hm Rng, N m C n, C M u

L + cnh nh

68

3439

Euphorbiaceae

Macaranga trigonostemonoides Croiz.

Ty Yn T , Sn ng, Bc Giang

L + cnh nh

69

2608

Loranthaceae

Macrosolen cochinchinensis (Lour.) Tiegh.

S Sn, S Th y, Kon Tum

L + thn

70

3011

Malpighiaceae

Malpighia glabra L.

C i Nc, C Mau

L + cnh nh

71

3306

Convolvulaceae

Merremia orientalis Ooststr.

Bn Ci, Dng Minh Chu, Ty Ninh

L + dy

72

2646

Rubiaceae

Metadina trichotoma (Zoll. & Mor.) Bakh. f.

Sa Th y, Kon Tum

L + cnh nh

73

3322

Annonaceae

Mitrephora thorelii Pierre

B Gia Mp, B nh Phc

L + cnh nh

74

3052

Moraceae

Morus alba L.

c Linh, Bnh Thun

L + cnh nh

75

3693

Fabaceae

Mucuna biplicata Teysm & Binn. Ex Kurz

c Ph, Tnh Linh, Bnh Thun

L + dy

76

3334

Rubiaceae

Mussaenda frondosa L.

B Gia Mp, B nh Phc

L + cnh nh

77

2841

Ranunculaceae

Naravelia laurifolia Wall. ex Hook. f.

VQG V Qu ng, H Tnh

L + dy

H khoa hc

Ni thu

Tn khoa hc

STT

Mu

B phn dng

78

3459

Fabaceae

Ormosia simplicifolia Merr. & Chun ex Merr.

Ty Yn T , Sn ng, Bc Giang

L + cnh nh

79

2884

Sapotaceae

Planchonella clemensii (Lecomte) P. Royen

K Sn, K Anh, H Tnh

L + cnh nh

80

3386

Caryophyllaceae

Polycarpaea corymbosa (L.) Lamk.

Hm Thun, Bnh Thun

L + thn

81

3441

Rosaceae

Prunus balansae (Koehne) J.E. Vidal

Ty Yn T , Sn ng, Bc Giang

L + cnh nh

82

2764

Fagaceae

Quercus franchetii Skan

L Dng, Lm ng

L + cnh nh

83

2931

Euphorbiaceae

Ricinus communis L.

Thng Xun, Thanh Ha

L + cnh nh

84

2921

Fabaceae

Saraca dives Pierre

Xun Kh ng, Nh Thnh, Th nh H

L + cnh nh

85

3006

Sonneratiaceae

Sonneratia caseolaris (L.) Engl.

C i Nc, C Mau

L + cnh nh

86

3471

Styracaceae

Styrax argentifolia H. L. Li

Ty Yn T , Sn ng, Bc Giang

L + cnh nh

87

2915

Euphorbiaceae

Suregada multiflora (A. Juss.) Baill.

Hi Vn, Nh Thnh, Th nh H

L + cnh nh

88

2871

Apocynaceae

Tabernaemontana bovina Lour.

VQG V Qu ng, H Tnh

L + cnh nh

89

2733

Rubiaceae

Tarenna hoaensis Pitard

E So, E k r, k L k

L + cnh nh

90

3421

Fabaceae

Tephrosia purpurea (L.) Pers.

VQG Ta Kou, Bnh Thun

L + cnh nh

91

3542

Combretaceae

Terminalia corticosa Pierre ex Gagnep.

Bun n, k L k

L + cnh nh

92

2971

Malvaceae

Thespesia populnea (L.) Soland. ex Correa

Tn Phong, Ra Gia, Bc Liu

L + cnh nh

93

3663

Meliaceae

Toona surenii (Blume) Merr.

Di Linh, Lm ng

L + cnh nh

94

3305

Asclepiadaceae

Toxocarpus villosus (Blume) Decne.

Dng Minh Chu, Ty Ninh

L + dy

95

3423

Tiliaceae

Triumfetta bartramia L.

Hm Thun Nam, Bnh Thun

Ton cy

96

2801

Sapotaceae

Xantolis barauensis (Lecomte) P. Royen

Vnh Hi, Ninh Hi, Ninh Thun

L + cnh nh

97

2763

Rutaceae

Zanthoxylum scandens Blume

L Dng, Lm ng

L + thn

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