BO CO THC HNH HA SINH

BI 1 LIPID
H v tn: ......
Lp:..
T:....
Ca:
Ngy thc tp:.

1. INH LNG CHOLESTROL TON PHN TRONG HUYT THANH

1.1. Nguyn tc:
Cholesterol ester + H2O
Cholesterol + O2

cholesterol + acid bo

cholesterol 3 one + H2O2

2H2O2 + 4 aminophenazone + phenol

1.2. Thuc th :
- Thuc th R :
m phosphate (pH 6,5)
4 Aminophenazone
Phenol
Peroxidase (POD)
Cholesterol esterase (CHE)
Cholesterol oxidase (CHO)
Natri azid
- Dung dch cholesterol chun:

quinoeimine + 4H2O

100 mmol/L
0,3 mmol/L
5 mmol/L
> 5 KU/L
> 150 U/L
> 100 U/L
0,05%
200 mg/dl (5,17 mmol/L)

1.3. Tin hnh :

Ly ba ng nghim
Thuc th R
Dd cholesterol chun (200mg/dl)
Huyt thanh

ng trng
1000l

ng chun
1000l
10l

ng o
1000l
10l

Trn u, trong 10 pht 20 25oC (hoc 5 pht 37oC). c mt quang ca ng th v

ng chun so vi ng trng bc sng 500nm.
1.4. Kt qu:
Cholesterol (mg/dl) =

D CHO
x CO = ..=
D OCHO

1.5. Bin lun:

Da vo nng cholesterol TP nh gi nguy c bnh tim mch. Cholesterol TP:
< 200 mg/dl: khng c nguy c x va ng mch.
200 250 mg/dl: nguy c va i vi bnh mch vnh.
250 300 mg/dl: nguy c cao i vi bnh mch vnh.
Vy:

2. NH LNG TRIGLYCERID TRONG HUYT THANH:

2.1 Nguyn tc:
Triglycerid c thy phn nh enzym lipase to ra glycerol. Sau khi c phosphoryl ha
nh glycerol kinase to glycerol 3 phosphate, glycerol - 3 - phosphate oxidase (GPO) oxi ha
cht ny to thnh hydrogen peroxide (H2O2). Phn ng ca H2O2 vi 4 aminoantipyrine di xc
tc ca enzym peroxidase to ra phc quinoneimine. Cng mu ca phc t l thun vi lng
triglyceride ban u.
Phn ng
Triglycerid + lypase glycerol + acid bo
Glycerol + ATP + GK glycerol 3 phosphate + ADP
Glycerol 3 phosphate + O2 + GPO dihydroxyaceton phosphat + H2O2
H2O2 + 4 aminoantipyrine + POD quinoneimine + HCl + H2O + 4-chlorophenol
2.2 Thuc th:
Thuc th R gm:
m PIPES (pH 7,5)
4 chlorophenol
4 aminoantipyrine
Magnesium ions
ATP
Lipases
Peroxidase
Glycerol kinase
Glycerol 3 phosphate oxidase
Dung dch triglycerid chun
Triglycerid

50 mmol/L
5 mmol/L
0,25 mmol/L
4,5 mmol/L
2 mmol/L
1,3 U/ml
0,5 U/ml
0,4 U/ml
1,5 U/ml
200 mg/dl hoc 2,28 mmol/L

2.3. Tin hnh:

Ly ba ng nghim
ng trng

ng chun

ng o
10l

Huyt thanh
Dd chun
10l
Thuc th R
1000l
1000l
1000l
o
o
Trn u, trong 10 pht 20 25 C (hoc 5 pht 37 C). c mt quang
ca ng o (Ao) v ng chun (Achun) so vi ng trng bc sng 546nm.
2.4. Kt qu:
Triglycerid [mg/dl] =

D TRI
x CO = ..=
D OTRI

2.5 Bin lun:

Bnh thng: Nam 50 130 mg/dl (0,57 1,50 mmol/L)
N 40 110 mg/dl (0,46 1,30 mmol/L)
Vy: