MUC LUC Trang Muc luc i Danh muec cdc bing vi Danh muc ede hinh vé viii Danh muc chit viét tat x Bat vin dé 1 Muc tiéu cud dé tai 2 TONG QUAN Chuong I - BAI CUONG VE CHAT MA TUY 3 1 Khai niém 3 IL, Nguén gée 3 IIL, Phan loai 4 IV. Cae chit ma tuy thudng gap 6 1. Thude phién va ede chat nhém opiat 6 LL. Thude phién 6 1.2. Cae chat nhém opiat ; 1.2.1, Morphine 7 1.2.2. Heroin 8 1.2.3. Codeine 9 2. Can sa va cde sin phém cud can sa 9 2.1, Cin sa 9 2.2. Céc sin phim 10 3. Cae chat kich thich thin kinh nhém amphetamine ll4. Cocaine 5. Cac chat ma tuy gay do gidc 6. Céc loai thuée an than gay ngit 6.1, Nhém benzodiazepine 6.2. Nhom barbiturate 7. MGt sO loai ma tuy téng hgp khac 7.1. Ketamine 7.2. Phethidine 7.3. Methadone Chung I - TONG QUAN VE MORPHINE L. Nguén géc IL. Cau tric hod hoe UL. M6t s6 tinh chit hod ly dic trung cud morphine 13 14 16 16 7 2 IV. Qua trinh chuyén hod sinh hoc tao thinh morphine tif heroin trong cd thé ngudi V. Téc dung cud morphine déi voi hé than kinh VI. Déc tinh cud morphine Chuong III - MOT SO PHUONG PHAP XAC DINH MORPHINE I. Phung phdp hod hoc 1. Véi thude thit Marquis’s Véi thude thts Mecke's 3. Vi thudc thi! HNO dam dic 4. Voi thude thir Dragendorrff IL. Phugng phap mién dich IIL. Phuong phip hod ly 1. Sic ky lép méng2. Sao ky khi 3. Sac ky lng cao dp 3.1. M6t s6 théng 36 co ban cud sie ky 3.2. Sdc ky long ghép kh6i phd IV. Cach thife bao quan va xi ly miu 1. Cach thife bao quan 2. Xitly miu 2.1, Thuy phan 2.2. Chiét xuat 34 35 37 41 43 43 45 45 45 V. Mét sO quy trinh tach chiét morphine cting nhu’ nhém opiat thudng dude sit dung trong phan tich ma tuy tai cdc phong thi nghiém cud lién hop quéc 1. Chiét léng- long, 2. Chiét pha rin THUC NGHIEM Chuong IV -HOA CHAT VA THIET BI 1, Hod chat 2. Dung cu 3. Thiét bi Chyong V - DIEU KIEN VAN HANH THIET BI 1. Gidi thiéu hé théng sic ky long ghép khéi phd cua hing Thermofini U1, Khdo sat diéu kién phan tich morphine 1, Khdo sat thanh phan pha dong Khio sit kha nang phan manh cud morphine véi ki thudt EST 3. Khdo sdt d6 tap lai cud tin higu 3.1. Tiém hanh 3.2. Két qua 46 46 46 iii4. Khdo sdt gidi han phat hién (LOD) va gidi han dinh lugng (LOQ) cud thiét bi 60 4.1, Tin hank, 60 Két qua 61 5. Lap day chudn 61 5.1. Tién hanh 61 5.2. Két qua 61 Chuong Vi - KHAO SAT DIBU KIEN TACH CHIET MORPHINE TREN MAU TU TAO BANG KI THUAT CHIET PHA RAN TREN COT SPE 66 1. Tao mau 66 2. Higu sudt thu hoi 66 2.1. Tién hanh: 66 2.2. két qua 68 3. Xée dinh gidi han phat hién (LOD) va gidi hun dinh lwgng (LOQ) trén nén mau nudc tiéu 70 3.1. Tién hamh 70 3.2. Két qua 70 Chuong VIL - UNG DUNG TREN MAU THAT VA KHAO SAT SY DAO THAI MORPHINE THEO THOI GIAN TRONG MAU NUGC TIEU CUA NGUOI SU DUNG MA TUY 2 1. Tiéz hanh B 2. Ket qua ' 4 BIEN LUAN KET QUA St KET LUAN 86 I. Phugng php phan tich , 86 1. Ki thuat chiét 86 iv2. Diéu kién chay may UL. Két qua dat duge trén cée mau thit IIL Huéng nghién citu méi TAL LIEU THAM KHAO PHU LUC 86 87 87 88 OL