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Tnh cht ca tia IR: Phn ln vt liu ngn cn tia sng thng th cng ngn c tia hng ngoi: g, giy, kim loi, nh sng thng khng th xuyn qua cc lp sng m, khi, my dy c nhng tia hng ngoi c th. Mi vt th c nhit cao hn 0 K u bc x tia hng ngoi. di sng (tn s) bc x ph thuc vo nhit ca vt. 2. Phng php xc nh cu trc ho hc bng quang ph (IR) My quang ph hng ngoi gn (FT-NIR) hot ng da trn s hp th bc x hng ngoi ca vt cht cn nghin cu. Phng php ny ghi nhn cc dao ng c trng ca cc lin kt ha hc gia cc nguyn t. Cc nhm chc khc nhau s c tn s hp th khc nhau v khng i nm trong vng t 4000 625 cm -1. Mi cht hu c c mt ph IR bao gm nhiu peak hp thu ca cc nhm chc. V vy, khi phn tch trn quang ph hng ngoi ta c th xc nh c cc nhm nguyn t m cht em phn tch c c. V d: Kt qu chp ph IR cho thy xut hin pic c bc sng t 3300 3600 cm-1, l ph dao ng ca nhm OH c lin kt hiro, v pic c bc sng t 1600 1800 cm-1 l ph ca nhm C=O trong nhm COOH tin cho vic s dng v biu din, thay v l bc sng hp thu t 2.5 n 16 m (vng IR gia) tc tn s t 1,9.1013 n 1,2.1014 Hz, trc honh s l s sng wavenumber c n v cm-1. Ba loi n v ny c th quy i ln nhau. Theo nh ngha: s sng = tn s/c vi tn s c n c l Hz tc s-1 c chnh l vn tc nh sng, c=3.1010 cm/s My quang ph FTIR c dng ch yu phn tch nh tnh (xc nh cu trc), nh lng hp cht hu c, cu trc ho hc ca hp cht v c. y l mt cng c nhanh v chnh xc phn tch mu lng, rn v vt liu keo m khng lm ph hy mu, tit kim chi ph bng cch gim thi gian thc hin v ha cht s dng. Mt s ng dng c th ca phng php IR: Gim nh php y. Xc nh vt liu ng nht. Hiu sut kt dnh. nh lng thit b c nh. Phn lp vt liu. n mn ho hc. 3. Kim nghim glucosamin sulfat natri clorua theo cc ch tiu cht lng ca Dc in M USP 26 +) nh tnh: - Kt qu: Hai ph IR ca cht th v chun c cc nh trng nhau - Trn sc k khi chy HPLC (theo mc ch nh lng) Pc chnh ca hai mu th v chun c thi gian lu nh nhau. - Ph 13C-NMR vi cc mi tng ng 4 loi C trong cu trc. - Ph 1H-NMR vi cc mi tng ng vi 3 loi H trong cu trc - Phn ng Cl : ng. +)Nng sut quay cc:

+ )Nhit nng chy c xc nh bng my DSC (Different Scanning Calori) +) Phn t lng ca sn phm c xc nh bng phng php sc k lc gel (GPC Gel permeation chromatography): 7.0464x105 g/mol +) pH : pH ca dung dch 20mg/ml bng nc ct khng c CO2 (un si ngui). Kt qa: 3,92 +)Mt khi lng do lm kh:Hm lng m trong ch phm t 0,95 %. +) Cn sau khi nung: 23%-25% +) Arsen: t 0,2 ppm +) Kim loi nng: Hm lng kim loi nng trong ch phm 0,001% (10ppm) +) nh lng: - nh lng sulfat: sulfat thc t t khong 16,7% t iu kin cho php l 16,3%-17,3%. - nh lng hm lng ch phm theo HPLC: hm lng ch phm t 80,55% (tnh theo nguyn trng) Thi gian lu mu th v mu chun trng nhau.