PHNG PHP TIN HNH Cc lu quan trng trc khi tin hnh thao tc: Trn u cc dung dch

ch trc khi s dng, trnh to bt kh. Vn np cc l ha cht tht k ngay sau khi s dng xong, nht l dung dch pEX2 v rt d bay hi. ln ht u tin, dung dch pEX2 s t ng chy ra khi u tip sau khi ht ln, do ht ln 1 ln ri nh xung li, sau ht ln li v tin hnh thao tc bnh thng. Ti cc bc yu cu trn u th hoc trn u, hoc vortex nh, khng c vortex mnh. Ti bc cho dung dch EX4 vo, em ly tm lun, khng trn hoc vortex. Sau mi bc ly tm, nht l ln ly tm u tin, phi ly tube ra khi my ly tm 1 cch nh nhng, trnh lm xo trn cc thnh phn trong tube, nu xo trn phi lp li bc ly tm. Sau ln ly tm u tin, trong tube s c 3 lp, lp di cng cha phenol, gia l protein bin tnh mu trng c v lp dch ni trong sut trn cha RNA. Khi thu dch ni, tuyt i trnh ht phi protein bin tnh, nu l ht phi th dng pipet y nh ngc tr li, phn dch cn li trong u tip khong 400 - 500l th vn chp nhn c. Ti bc ta vi dung dch pEX3, c th n vi gi ngn ng t lnh hoc -20oC. Ti cc bc loi b dch ni c th hoc dng pipet 200l ht sch, phn cn c th bong ra v tri di, do phi cn thn trnh loi b lun phn cn. Ti bc 60oC, nu RNA dng cho phn ng PCR th phi cho n khi kh hon ton. Trnh chm thn pipet vo l ha cht, trnh phn np bn trong ca tube hoc phn ming ca cc l ha cht chm vo gng tay hoc bt k vt g khc. Nu v l do g m cc l ha cht b nhim phi mu ang thao tc, phi thay ha cht mi ngay, tuyt i khng c tn dng. Sau khi tin hnh xong, ct ha cht ngay ri mi thc hin cc bc tip theo. i vi cc xt nghim lin quan n bnh Lao: nh du cc tube 1.5ml biopure bng k hiu mu. Cho 100l mu vo 1 tube biopure c sn 900l dung dch pER1, vortex 30s, yn 10 pht. Thm vo 200l dung dch pEX2, trn u sao cho ton b dung dch trong tube phi chuyn thnh mu trng c. Ly tm 13000 vng/pht trong 10 pht. Thu 600l dch ni c cha RNA vo 1 tube biopure c sn 600l dung dch pEX3. Trn u, yn 10 pht. Ly tm 13000 vng/pht trong 10 pht. Loi b dch ni, thu cn mu xanh (hoc hng). Cho t t 900l dung dch EX4 vo. Ly tm 13000 vng/pht trong 5 pht. Loi b dch ni, thu cn. kh 60oC trong 10 - 15 pht. Cho vo 100l dung dch ER5. Trc khi s dng, dng pipet trn u cho n khi phn cn tan hon ton. Bo qun mu RNA -20oC. Mun lu tr lu phi bo qun -70oC vi RNase inhibitor. i vi cc xt nghim khc: nh du cc tube 1.5ml biopure bng k hiu mu. Cho 200l mu vo 1 tube biopure c sn 900l dung dch pER1, vortex 30s, yn 10 pht. Thm vo 200l dung dch pEX2, trn u sao cho ton b dung dch trong tube phi chuyn thnh mu trng c. Ly tm 13000 vng/pht trong 10 pht. Thu 600l dch ni c cha RNA vo 1 tube biopure c sn 600l dung dch pEX3. Trn u, yn 10 pht. Ly tm 13000 vng/pht trong 10 pht. Loi b dch ni, thu cn mu xanh (hoc hng). Cho t t 900l dung dch EX4 vo. Ly tm 13000 vng/pht trong 5 pht. Loi b dch ni, thu cn. kh 60oC trong 10 - 15 pht. Cho vo 50l dung dch ER5. Trc khi s dng, dng pipet trn u cho n khi phn cn tan hon ton. Bo qun mu RNA -200C. Mun lu tr lu phi bo qun -70oC vi RNase inhibitor. Lu : B kit tch chit DNA (VA.A92-002A) v b kit tch chit RNA (VA.A92-002B) bng phng php phenol/chloroform do cng ty Vit sn xut c 1 s ha cht gng nhau v quy trnh ging ht nhau, do c th tch chit DNA v RNA cng lc, cc ha cht ging nhau c th dng chung nhng phi s dng u tip biopure ht ha cht.

PHNG PHP TIN HNH Cc lu quan trng trc khi tin hnh thao tc: Trn u cc dung dch trc khi s dng, trnh to bt kh. Vn np cc l ha cht tht k ngay sau khi s dng xong, nht l dung dch pEX2 v rt d bay hi. ln ht u tin, dung dch pEX2 s t ng chy ra khi u tip sau khi ht ln, do ht ln 1 ln ri nh xung li, sau ht ln li v tin hnh thao tc bnh thng. Ti cc bc yu cu trn u th hoc trn u, hoc vortex nh, khng c vortex mnh. Ti bc cho dung dch EX4 vo, em ly tm lun, khng trn hoc vortex. Sau mi bc ly tm, nht l ln ly tm u tin, phi ly tube ra khi my ly tm 1 cch nh nhng, trnh lm xo trn cc thnh phn trong tube, nu xo trn phi lp li bc ly tm. Sau ln ly tm u tin, trong tube s c 3 lp, lp di cng cha phenol, gia l protein bin tnh mu trng c v lp dch ni trong sut trn cha RNA. Khi thu dch ni, tuyt i trnh ht phi protein bin tnh, nu l ht phi th dng pipet y nh ngc tr li, phn dch cn li trong u tip khong 400 - 500l th vn chp nhn c. Ti bc ta vi dung dch pEX3, c th n vi gi ngn ng t lnh hoc -20oC. Ti cc bc loi b dch ni c th hoc dng pipet 200l ht sch, phn cn c th bong ra v tri di, do phi cn thn trnh loi b lun phn cn. Ti bc 60oC, nu RNA dng cho phn ng PCR th phi cho n khi kh hon ton. Trnh chm thn pipet vo l ha cht, trnh phn np bn trong ca tube hoc phn ming ca cc l ha cht chm vo gng tay hoc bt k vt g khc. Nu v l do g m cc l ha cht b nhim phi mu ang thao tc, phi thay ha cht mi ngay, tuyt i khng c tn dng. Sau khi tin hnh xong, ct ha cht ngay ri mi thc hin cc bc tip theo. i vi cc xt nghim lin quan n bnh Lao: nh du cc tube 1.5ml biopure bng k hiu mu. Cho 100l mu vo 1 tube biopure c sn 900l dung dch pER1, vortex 30s, yn 10 pht. Thm vo 200l dung dch pEX2, trn u sao cho ton b dung dch trong tube phi chuyn thnh mu trng c. Ly tm 13000 vng/pht trong 10 pht. Thu 600l dch ni c cha RNA vo 1 tube biopure c sn 600l dung dch pEX3. Trn u, yn 10 pht. Ly tm 13000 vng/pht trong 10 pht. Loi b dch ni, thu cn mu xanh (hoc hng). Cho t t 900l dung dch EX4 vo. Ly tm 13000 vng/pht trong 5 pht. Loi b dch ni, thu cn. kh 60oC trong 10 - 15 pht. Cho vo 100l dung dch ER5. Trc khi s dng, dng pipet trn u cho n khi phn cn tan hon ton. Bo qun mu RNA -20oC. Mun lu tr lu phi bo qun -70oC vi RNase inhibitor. i vi cc xt nghim khc: nh du cc tube 1.5ml biopure bng k hiu mu. Cho 200l mu vo 1 tube biopure c sn 900l dung dch pER1, vortex 30s, yn 10 pht. Thm vo 200l dung dch pEX2, trn u sao cho ton b dung dch trong tube phi chuyn thnh mu trng c. Ly tm 13000 vng/pht trong 10 pht. Thu 600l dch ni c cha RNA vo 1 tube biopure c sn 600l dung dch pEX3. Trn u, yn 10 pht. Ly tm 13000 vng/pht trong 10 pht. Loi b dch ni, thu cn mu xanh (hoc hng). Cho t t 900l dung dch EX4 vo. Ly tm 13000 vng/pht trong 5 pht. Loi b dch ni, thu cn. kh 60oC trong 10 - 15 pht. Cho vo 50l dung dch ER5. Trc khi s dng, dng pipet trn u cho n khi phn cn tan hon ton. Bo qun mu RNA -200C. Mun lu tr lu phi bo qun -70oC vi RNase inhibitor. Lu : B kit tch chit DNA (VA.A92-002A) v b kit tch chit RNA (VA.A92-002B) bng phng php phenol/chloroform do cng ty Vit sn xut c 1 s ha cht gng nhau v quy trnh ging ht nhau, do c th tch chit DNA v RNA cng lc, cc ha cht ging nhau c th dng chung nhng phi s dng u tip biopure ht ha cht.

Nu qu khch c nhu cu hay cn s h tr c bit xin vui lng gi s: 0909.683.483 (Mr. Vit), 0903.217.541 (Ms. Thy) hoc 0918.677.220 (Mr. Huy), chng ti cam kt s lm hi lng qu khch!

Nu qu khch c nhu cu hay cn s h tr c bit xin vui lng gi s: 0909.683.483 (Mr. Vit), 0903.217.541 (Ms. Thy) hoc 0918.677.220 (Mr. Huy), chng ti cam kt s lm hi lng qu khch!