Hydrogen sulfide (H2S)

Phng php Iodine Nguyn tc H2S trong mu nc c cha H2S s b kt ta CdS khi phn ng vi CdCl2. CdCl2 + H2S = CdS + 2HCl Sau CdS c ha tan bng mt lng tha I2 chun, trong mi trng acid. CdS + I2 + 2HCl = S + CdCl2 + 2HI. Lng tha I2 c xc nh bng phng php chun vi dung dch Na2S2O3 tiu chun v h tinh bt c dng lm cht ch th xc nh im tng ng, ging nh qu trnh xc nh oxy ha tan trong nc bng phng php Winkler. I2 + I2 tinh bt + 2Na2S2O3 = Na2S4O6 + 2NaI + tinh bt. (mu xanh) Thu v bo qun mu Tin hnh thu mu trong chai nt mi nu, c nh mu bng 1mL CdCl2 Thuc th Dung dch CdCl2 2%: Ha tan 2 g CdCl2 vi nc ct thnh 100mL. Dung dch HCl 4M: Cho 25mL HCl c (12M) vo v pha long thnh 100mL vi nc ct. Dung dch I2 0,1N: Ha tan 80 g KI trong 500mL nc ct khng, tip tc cho vo12,7 g I2 khuy u cho tan ht, sau pha thnh 1.000mL. Dung ny sau khi pha xong phi xc nh li nng chnh xc bng dung dch Na 2S2O3 0,1N tiu chun. Cch tin hnh nh sau: dng bnh tam gic 100mL, cho vo 20mL dung dch I2 va pha trn (dng buret xc nh th tch I2, khng dng ng ht), dng dung dch Na2S2O3 0,1N tiu chun, chun cho n khi dung dch c mu vng, tip tc chun cho n khi dung dch c mu vng nht, cho 3 git h tinh bt, lc u dung dch c mu xanh, tip tc chun cho n khi dung dch chuyn t mu xanh sang khng mu th dng li, ghi th tch dung dch Na2S2O3 0,1N s dng. Lm li nh trn mt ln na ly gi tr V trung bnh. Sau hiu chnh nng dung dch I 2 cho chnh xc bng biu thc: N1V1 = N2V2 Dung dch I2 0,01N: ly 50mL I2 0,1N dng nc ct pha long thnh 500mL. Dung dch Na2S2O3 tiu chun 0,1N: Pha mt ng Na2S2O3 tiu chun 0,1N trong 1000mL nc ct Dung dch Na2S2O3 0,01N: S dng cng thc N1V1 = N2V2 pha dd c th nh sau: Ly 50mL dung dch Na2S2O3 0,1N pha long vi nc ct thnh 500mL. (khng mu)

Ch th h tinh bt 1%: Ho tan 0,49 g K2S2O3 trong 100mL nc m (t 80900C) khuy u cho n khi dung dch mu trong sut, cho vo 0,5mL formaline nguyn cht s dng c lu. Tin hnh Thu mu nc vo 2 l nt mi 125mL, sau m np l ra, cho ln lt vo mi l 1mL dung dch CdCl2 2%, y np l li lc u mt ln na, yn 24 gi (nu c H2S s c kt ta mu vng di y bnh). M np l ra dng ng cao su ht b phn nc trong trn kt ta (ch : khi ht cn ng cao su gn mt nc ch khng c cm su vo y bnh v cho nc chy nh ra ngoi, nu nc chy mnh kt ta b vn ln v cun tri ra ngoi, lm kt qu khng chnh xc ). Ha tan kt ta bng 5mL HCl 4M v 5mL dung dch I2 0,01N. Chuyn dung dch t l nt mi sang bnh tam gic 100mL, trng l nt mi bng 30mL nc ct, nc ct ny cng cho vo bnh tam gic. Dng dung dch Na2S2O3 0,01N chun cho n khi dung dch mu vng nht, cho vo 3 git ch th h tinh bt, lc u dung dch c mu xanh sau tip tc chun t t cho n khi dung dch chuyn t mu xanh sang khng mu th dng li, ghi tng th tch V1 (mL) dch Na2S2O3 0,01N s dng. Lm tng t nh trn cho l cn li, ghi th tch V2(mL) dch Na2S2O3 0,1N s dng. Tnh VTB = (V1+V2)/2. Bnh chun: Dng 2 bnh tam gic 100mL, ln lt cho vo tng bnh cc ha cht nh sau: 30mL nc ct, 5mL HCl 4M v 5mL dung dch I2 0,01N, lc u dung dch c mu vng nu . Dng dung dch Na2S2O3 0,01N chun cho n khi dung dch tr nn mu vng nht, cho 3 git ch th h tinh bt vo, lc u dung dch c mu xanh, tip tc chun cho n khi dung dch chuyn t mu xanh sang khng mu th dng li ghi th tch V0 Na2S2O3 0,01N s dng. Lm tng t nh trn cho bnh cn li ly th tch V0 trung bnh. Tnh kt qu
H 2 S (mg / L) = (V0 VTB ) x N x 17 x 1000 125

VTB: Th tch trung bnh dung dch Na2S2O3 s dng trong 2 ln phn tch mu nc V0: th tch trung bnh ca dung dch Na2S2O3 trong 2 ln phn tch mu trng N: Nng ng lng ca dung dch Na2S2O3 s dng.

17: ng lng g ca H2S. Phng php Methylene blue Nguyn tc Nguyn tc ca phng php ny da trn phn ng ca sulfide (S2-), ferric chloride v dimethyl-p-phenylenediamine to nn methylene blue. Ammonium phosphate c thm vo sau khi hin mu kh mu ca ferric chloride. Sau nng S2- c xc nh bi my so mu quang ph bc sng 664 nm. Thu mu v bo qun Tin hnh thu mu trong chai nt mi nu, bo qun lnh 4oC Thuc th Dung dch PRE 1: 500mL HCl 37% pha thnh 1000mL vi nc ct. Dung dch A: Ha tan 4g C8H14Cl2N2 trong PRE 1 thnh 1000mL. Dung dch B: Ha tan 16g FeCl3.6H2O trong PRE 1 thnh 1000mL. Dung dch chun Dung dch Na2S.9H2O 100mg/L: ha tan 0,750g Na2S.9H2O trong 1000mL nc ct khng oxy (nc ct khng oxy: un nc ct ln si khong 10 pht em khi bp bt kn ngay ). Dung dch Na2S.9H2O 5mg/L: ha tan 5mL dd Na2S.9H2O 100mg/l vi nc ct thnh 100mL Thit lp mu chun Bng 7.7. Cc bc thit lp mu chun phn tch H2S theo phng php Methylene blue STT Nng mu Th tch dung dch chun Th tch nc ct chun (mg/L) Na2S.9H2O 5mg/L (mL) (mL) 1 0,00 0,0 100,0 2 0,02 0,4 99,6 3 0,04 0,8 99,2 4 0,06 1,2 98,8 5 0,08 1,6 98,4 6 0,10 2,0 98,0 Tin hnh ong 20mL mu nc. Ln lt cho thuc th vo 1mL thuc th A, 1mL thuc th B Ch 5 pht khi mu xanh xut hin chng ta em o hp th quang bc sng 665nm. Tnh kt qu Tng ng vi nng C ca mu chun ta c hp th quang A. Da vo s tng quan ny chng ta c th lp phng trnh tng quan dng A = aC + b

Trong : A: hp th quang C : nng cht chun (mg/L) Sau khi thit lp phng trnh, chng ta o hp th quang ca mu cn phn tch v th vo phng trnh chng ta s tnh c nng ca S2- c trong mu nc.
C= Ab a

tnh hm lng H2S khi thu mu nc chng ta phi o pH v nhit . Da vo bng tra (xem Chng 3, Mc 5, Bng 3.5) xc nh t l phn trm ca H 2S cha trong tng sulfide, t tnh ra hm lng H2S.