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NGHIN CU CH TO HT NANO BC V KH
NNG ST KHUN CA N
H NI - 2011
I HC QUC GIA H NI
TRNG I HC CNG NGH
NGHIN CU CH TO HT NANO BC V KH
NNG ST KHUN CA N
H NI - 2011
Li cm n
Li cam oan
Ti xin cam oan lun vn ny l cng trnh do ti t lm v nghin cu, cc trch dn ti
u ghi r trong phn ti liu tham kho. Nu c bt k vn g xy ra, ti xin chu
hon ton trch nhim
Ngi vit lun vn:
Nguyn Ngc Hng
MC LC
Nguyn Ngc Hng................................................................................5
H NI - 200<s cui ca nm bo v KLTN>.......................................................5
M2......................................................................................................................29
M1......................................................................................................................29
M2......................................................................................................................29
M1......................................................................................................................29
M2......................................................................................................................29
0.5......................................................................................................................29
0.154..................................................................................................................29
0.173..................................................................................................................29
0.129..................................................................................................................29
0.121..................................................................................................................29
0.130..................................................................................................................29
0.118..................................................................................................................29
0.145..................................................................................................................29
0.133..................................................................................................................29
0.151..................................................................................................................29
0.148..................................................................................................................29
1.0......................................................................................................................29
0.181..................................................................................................................29
0.209..................................................................................................................29
0.123..................................................................................................................29
0.124..................................................................................................................29
0.129..................................................................................................................29
0.133..................................................................................................................29
0.139..................................................................................................................29
0.138..................................................................................................................29
0.148..................................................................................................................29
0.149..................................................................................................................29
1.5......................................................................................................................29
0.252..................................................................................................................29
0.301..................................................................................................................29
0.125..................................................................................................................29
0.120..................................................................................................................29
0.127..................................................................................................................29
0.119..................................................................................................................29
0.142..................................................................................................................29
0.136..................................................................................................................29
0.151..................................................................................................................29
0.152..................................................................................................................29
2.0......................................................................................................................29
0.421..................................................................................................................29
0.444..................................................................................................................29
0.131..................................................................................................................29
0.124..................................................................................................................29
0.126..................................................................................................................29
0.131..................................................................................................................29
0.141..................................................................................................................29
0.145..................................................................................................................29
0.150..................................................................................................................29
0.162..................................................................................................................29
2.5......................................................................................................................29
0.610..................................................................................................................29
0.673..................................................................................................................29
0.117..................................................................................................................29
0.129..................................................................................................................29
0.128..................................................................................................................29
0.121..................................................................................................................29
0.143..................................................................................................................29
0.128..................................................................................................................29
0.151..................................................................................................................29
0.154..................................................................................................................29
3.0......................................................................................................................29
0.749..................................................................................................................29
0.877..................................................................................................................29
0.126..................................................................................................................29
0.122..................................................................................................................29
0.125..................................................................................................................29
0.119..................................................................................................................29
0.144..................................................................................................................29
0.140..................................................................................................................29
0.151..................................................................................................................29
0.155..................................................................................................................29
3.5......................................................................................................................29
1.262..................................................................................................................29
1.170..................................................................................................................29
0.409..................................................................................................................29
0.127..................................................................................................................29
0.128..................................................................................................................29
0.122..................................................................................................................29
0.146..................................................................................................................29
0.140..................................................................................................................29
0.155..................................................................................................................29
0.154..................................................................................................................29
4.0......................................................................................................................29
1.372..................................................................................................................29
1.389..................................................................................................................29
0.144..................................................................................................................29
0.135..................................................................................................................29
0.128..................................................................................................................29
0.123..................................................................................................................29
0.145..................................................................................................................29
0.141..................................................................................................................29
0.156..................................................................................................................29
0.155..................................................................................................................29
4.5......................................................................................................................29
1.490..................................................................................................................29
1.521..................................................................................................................29
0.142..................................................................................................................29
0.135..................................................................................................................29
0.137..................................................................................................................29
0.121..................................................................................................................29
0.153..................................................................................................................29
0.149..................................................................................................................29
0.156..................................................................................................................29
0.157..................................................................................................................29
5.0......................................................................................................................29
1.612..................................................................................................................29
1.645..................................................................................................................29
0.143..................................................................................................................29
0.139..................................................................................................................29
0.133..................................................................................................................29
0.121..................................................................................................................29
0.148..................................................................................................................29
0.151..................................................................................................................29
0.155..................................................................................................................29
0.157..................................................................................................................29
5.5......................................................................................................................29
1.675..................................................................................................................29
1.713..................................................................................................................29
0.107..................................................................................................................29
0.107..................................................................................................................29
0.081..................................................................................................................29
0.077..................................................................................................................29
0.104..................................................................................................................29
0.010..................................................................................................................29
0.111..................................................................................................................29
0.108..................................................................................................................29
3.3 So snh vi phng php o bn knh vng v khun..................................34
Chng 4: KT LUN.......................................................................35
Lnh vc
Tnh cht
Tnh cht
in
Bc sng in t
Qung ng t do trung bnh khng n
hi
Hiu ng ng ngm
dy vch men
Qung ng tn x spin
H lng t
di suy gim
su b mt kim loi
di lin kt cp Cooper
thm thu Meisner
Tng tc bt nh x
Bin ht
Bn knh khi ng t v
Sai hng mm
nhn b mt
Hnh hc topo b mt
di Kuhn
Cu trc nh cp
Cu trc tam cp
Nhn bit phn t
Tnh cht t
Tnh cht
quang
Tnh siu
dn
Tnh cht c
Xc tc
Siu phn t
Min dch
di ti hn
(nm)
10-100
1-100
1-10
10-100
1-100
1-100
10-100
10-100
0,1-100
1-100
1-1000
1-10
1-100
0,1-10
1-10
1-10
1-100
1-10
10-1000
1-10
Chn on: S dng cc ht nano (ht nano vng, nano t, chm lng t)
nh du cc phn t sinh hc, vi sinh vt, pht hin cc chui gen nh vo
c ch bt cp b xung ca DNA hoc c ch bt cp khng nguyn khng
th.
Vn chuyn thuc: Cung cp thuc cho tng t bo c th bng cch s dng
cc ht nano nhm tit kim thuc v trnh cc tc dng ph.
M k thut: Cng ngh nano c th gip c th ti sn xut hoc sa cha
cc m b h hng bng cch s dng gin da trn vt liu nano v cc yu
t tng trng[7].
1.2 Ht nano bc
1.2.1 Gii thiu v bc kim loi
Cu hnh electron ca bc: 1s22s22p63s23p63d104s24p64d105s1
Bn knh nguyn t Ag: 0,288 nm
Bn knh ion bc: 0,23 nm
Bng 2: S nguyn t bc trong mt n v th tch[14].
Kch thc ca ht
nano Ag (nm)
S nguyn t cha
trong
31
3900
20
250000
Tnh kh khun, chng nm, kh mi, c kh nng pht x tia hng ngoi i
xa, chng tnh.
Khng c hi cho sc khe con ngi vi liu lng tng i cao, khng c
ph gia ha cht.
n nh nhit cao.
10
Cc dng c cha thc phm: Nhng dng bng nha c pha thm ht
nano bc c tc dng kh trng. Qua kim tra cho thy chng c kh nng dit
99.9% vi khun.
11
- Y t:
+ Khu trang nano bc: c thit k vi 3-4 lp gm 2 lp vi, mt lp vt liu
tm nano bc v than hot tnh gia, loi khu trang ny c kh nng dit khun, dit
virus, lc khng kh rt tt. Lp vi tm nano bc c chc nng dit vi khun, virus, nm
b gi li trn khu trang ng thi c tc dng kh mi.
13
Cc sn phm khc:
14
Giai on tim pht di hay ngn lin quan n bn thn tng loi vi sinh vt v
tnh cht ca mi trng. Nu tnh cht ha hc ca mi trng mi sai khc nhiu vi
15
Vic tiu hao cht dinh dng v vic tch ly cc cht thi c hi s lm tn tht
n mi trng sng ca vi sinh vt, lm cho s lng t bo sng gim xung. l c
im ca giai on t vong.
Ging nh giai on logarit, s t vong ca qun th vi sinh vt cng c tnh
logarit (t l t bo cht trong mi gi l khng i). Tng s t bo sng v t bo cht
khng thay i v cc t bo cht cha b phn hy. Mun xc nh s lng t bo sng
phi pha long ra ri cy ln thch a v a vo iu kin thch hp xc nh s
khun lc xut hin.
Mc du phn ln vi sinh vt t vong theo phng thc logarit nhng sau khi s
lng t bo t nhin gim xung th tc cht ca t bo chm li. l do mt s c
th sng li nh c tnh khng c bit mnh. V iu ny v nhng nguyn nhn khc
lm cho ng cong ca giai on t vong c th kh phc tp[8].
1.3.2 Xc nh s sinh trng ca vi khun
1.3.2.1 Xc nh s lng t bo
Phng php n gin nht xc nh s lng t bo l m trc tip di knh
hin vi. Dng cc phng m m va nhanh chng, d dng, li r tin nht, li c
th quan st thy kch c v hnh dng t bo.
Vi t bo vi khun cn nhum mu hoc l dng knh hin vi tng phn pha hay
knh hin vi hunh quang (phase-constrast or fluoresence microscope) d quan st
hn. Phng m c cu trc c mt su nht nh li c chia ra thnh cc nh.
Khi m s lng ta a dch pha long vo phng m, y l knh (lamelle/ cover
glass) ln trn, sau tin hnh m s lng di knh hin vi.
Khuyt im ca phng php ny l khng xc nh c vi cc mu c s
lng vi khun qu nh, chnh xc cng khng cao v khng phn bit c gia t
bo sng v t bo cht[8].
17
nhau th tng lng cht trong t bo c tng quan trc tip vi tng sinh khi vi
sinh vt[8].
19
- Ly 1.7 g bc nitrat AgNO3 (dng mui kt tinh mu trng) ha tan trong 100ml
nc ct.
- Thm vo dung dch 0.62 g natri hydroxyt, NaOH, to kt ta Ag 2O c mu
en.
- Ha tan kt ta bng mt lng va amoni hydroxyt, NH4OH, to ra dung
dch phc bc Ag(NH3)2OH trong sut.
- Cho thm mt lng cht hot ng b mt axit oleic v khuy u trn my
khuy t trong 2 gi nhit phng to ra dung dch ng nht v c nht cao.
- Sau thm 2.0g ng glucozo v khuy u trn my khuy t thc hin
phn ng kh. Trong qu trnh kh s dng n bc x UV kch thch phn ng kh
v iu khin phn b kch thc ht nano bc.
21
nAg0
-
Trong :
-
A l mt quang ti bc sng .
2.2.3.3 o OD
Lp li cc bc trn 10 ln
25
26
Phn tch ph UV-VIS ca mu dung dch c nng 100ppm cho thy s xut
hin nh hp th cc i ti bc sng 422nm. iu ny cho thy s c mt ca
cc ht nano bc trong dung dch.
Kt qu phn tch t nh TEM cho thy cc ht nano trong dung dch c hnh cu vi
kch thc trung bnh khong 9-10 nm. Cc ht nano bc phn tn u, khng b kt m
cho thy hiu qu ca vic s dng axit oleic lm cht hot ng b mt.
27
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
0(g/ml)
M1
M2
0.154
0.173
0.181
0.209
0.252
0.301
0.421
0.444
0.610
0.673
0.749
0.877
1.262
1.170
1.372
1.389
1.490
1.521
1.612
1.645
1.675
1.713
KT QU O OD
5(g/ml)
10(g/ml)
M1
M2
M1
M2
0.129 0.121 0.130 0.118
0.123 0.124 0.129 0.133
0.125 0.120 0.127 0.119
0.131 0.124 0.126 0.131
0.117 0.129 0.128 0.121
0.126 0.122 0.125 0.119
0.409 0.127 0.128 0.122
0.144 0.135 0.128 0.123
0.142 0.135 0.137 0.121
0.143 0.139 0.133 0.121
0.107 0.107 0.081 0.077
15(g/ml)
M1
M2
0.145 0.133
0.139 0.138
0.142 0.136
0.141 0.145
0.143 0.128
0.144 0.140
0.146 0.140
0.145 0.141
0.153 0.149
0.148 0.151
0.104 0.010
25(g/ml)
M1
M2
0.151 0.148
0.148 0.149
0.151 0.152
0.150 0.162
0.151 0.154
0.151 0.155
0.155 0.154
0.156 0.155
0.156 0.157
0.155 0.157
0.111 0.108
t (gi)
Hnh 26: ng cong sinh trng ca E. coli khi khng c mt ca nano bc
29
a)
OD
t (gi)
Hnh 27: ng cong sinh trng ca E. coli khi c s xut hin ca nao bc
a) Nng 5(g/ml)
OD
t (gi)
b) Nng 10(g/ml)
30
OD
c)
t (gi)
c) Nng 15(g/ml)
d
OD
t (gi)
d) Nng 25(g/ml)
31
OD
t (gi)
Hnh 28: ng cong sinh trng ca E. coli trong tt c cc mu
Hnh 29: Cc bnh nui sau 8 gi: Ta thy bnh khng c cha ht nano (ngoi cng bn
phi) b vn c chng t vi khun pht trin mnh. Cc bnh cn li c cha cc ht nano bc
bnh gn nh khng i mu.
33
34
Chng 4: KT LUN
Trong khun kh kha lun ny, chng ti :
1. Th nghim ch to ht nano bc dng dung dch theo cc nng t 5 g/ml
n 25 g/ml.
2. Nghin cu tc ng ca nano bc ln s pht trin ca vi khun bng k thut
OD (trong 11 gi).
3. Chng minh c rng cc mu nano bc dng dung dch u th hin kh
nng khng khun mnh vi vi khun E. coli.
nh hng, xut cho cc nghin cu tip theo:
1. Th nghim tnh khng khun ca nano bc vi cc chng vi khun gy bnh
khc.
2. Xc nh nng ti thiu ca ht nano bc dung dch c tc dng khng
khun
3. Nghin cu c ch tc ng ca ht nano bc ln vi khun.
Ting vit
[3] Cng ngh nano. Vi.wikipedia.org
http://vi.wikipedia.org/wiki/C%C3%B4ng_ngh%E1%BB%87_nano
[4] Cng ngh nano v nhng ng dng trong thc tin, Trang 1
www.prt.vn/upload/Nghiencuu/Congnghenanovaungdung.doc
[5] Gii thiu v Knh hin vi. Svtunhien.net, Trang 1
http://svtunhien.net/mybb/printthread.php?tid=693
[6] Nguyn Hong Hi, Trung tm Khoa hc Vt liu, Trng i hc Khoa hc
T nhin, i hc Quc gia H Ni. Cc ht nano kim loi. Tp ch
http://vatlyvietnam.org, 2007. Trang 9.
[8] Sinh trng v pht trin ca vi sinh vt. Maxreading.com
http://maxreading.com/sach-hay/vi-sinh-vat/sinh-truong-va-phat-trien-cua-vi-sinh
vat-37171.html
[13] Nguyn Minh Tr, Mt phng php nh gi kh nng khng khun ca
Chitozan, Trang 1
[14] Nguyn Ngc T. Nghin cu gel nc thng minh nhy pH lai nano bc.
Kha lun tt nghip i hc chnh quy 2009. Trang 8-9.
Ting Anh
36
[1] Anh-Tuan Le*, P.T. Huy, Phuong Dinh Tam, Tran Quang Huy, Phung Dac
Cam, A.A. Kudrinskiy, Yu A. Krutyakov, Green synthesis of finely-dispersed
highly bactericidal silver nanoparticles via modified Tollens technique, Current
Applied Physics, Vol. 10, pp. 910-916.
[2] Ales Pana cek, Milan Kola r, Renata Vecerova, Robert Prucek, Jana
Soukupova, Vladimr Krystof , Petr Hamal, Radek Zboril, Libor Kvtek,
Antifungal activity of silver nanoparticles against Candida spp, Biomaterials 30
(2009), pp. 63336340.
[7] List of nanotechnology applications. en.wikipedia.org
http://en.wikipedia.org/wiki/List_of_nanotechnology_applications
[9] Siddhartha Shrivastava, Tanmay Bera, Arnab Roy, Gajendra Singh, P
Ramachandrarao and Debabrata Dash, Characterization of enhanced antibacterial
effects of novel silver nanoparticles, Nanotechnology 18 (2007) 225103, pp 9.
[10] Silver. En.wikipedia.org
http://en.wikipedia.org/wiki/Silver
[11] Silver Nanoparticles: A Case Study in Cutting Edge Research. Cnx.org
http://cnx.org/content/m19597/latest/
[12] Preparation of Silver Nanoparticles and Their Characterization. Azonano.com
http://www.azonano.com/article.aspx?ArticleID=2318
[15] http://www.ncbi.nlm.nih.gov/pubmed/3807907
37