K thut bao gm: 3 giai on.

Gi m cho nm

Nm rm ang pht trin

1/ Meo ging cp 1: Giai on u tin a/ Mi trng cp 1: Mi trng cn bn thng dng nht l P.D.A gm khoai ty 300g, Glucose 20g, Agar 20g, Nc ct sch cho 1 lt. Khoai ty ra sch ct khi vung nh 1 cm3 nu chn lc xc ly nc, cho Agar vo nc khoai ty nu v khuy cho tan u, thm glucose vo v b sung nc cho 1 lt. Sau khi kim tra pH xong cho vo ng nghim. ngui, lm nt bng qun giy bao nt li. Hp kh trng p sut 0,8 1 atm trong 1 gi. b/ Phn lp ging nm: Ging thun c th thu nhn t nhiu ngun khc nhau, ch yu t bo t hoc t m tht nm. Nm rm: Chn tai nm dng hnh trng, gt sch gc,lau nm v tay ngi cy bng Alcool, kh trng dao cy, x i tay nm, dng dao ct 1 ming phn thn gn m nm cho vo ng nghim tin hnh iu kin v trng vi n cn. c/ t: t ni m, 4-5 ngy y ng nghim; t nm thun pht trin, y ng th nhn nhiu ra bng cch cy truyn. Khng c cy truyn ging qu 3 ln.

T nm trn mi trng cp 1 ( nh: Xun ng) 2/ Meo ging cp 2: Thng gi l meo b l dng ging chuyn tip sang meo thnh phm cp 3. Mc ch ca meo b: cy meo b di c t thng trong bch meo cp cp III gip meo pht trin ng u, si meo trong bch c cng tui. a/ Nguyn liu: Chn rm la ma c cng di, thch hp t pht trin, tut b bt l, ct khc khong 12cm, ly 8-10 cng dng dy nylon tc nh ct qun chung quanh thnh 1 b nh. Hoc thn cy m: La thn cy m gi rc ht v xanh, cht khc khong 12cm ch thnh thanh nh, rc b rut, phi nng cho tht kh. Bo qun 1 thi gian ngn khng lu qu d b mt. Hoc c th dng la. b/ Mi trng: 1kg rm b (thn m) (la) ngm trong nc vi 1%, bt bp 150g v cm 50g nu c trn vo rm b (thn m) (la). c/ p kh trng: 1,5 atm/gi. d/ Cy meo: T ng nghim meo cp I, dng dao cy ct 1 phn thch c meo chuyn vo chai meo b trong iu kin v trng. e/ meo: 15-25 ngy to 30-35oC f/ Chn ging: Chn chai pht trin nhanh khng bch; loi b chai xu, bch.

T nm trn mi trng cp 2 (nh: Xun ng) 3/ Meo ging cp 3 a/ Bao b: lm meo dng chai thy tinh trong sut hay bao ti PP kch thc nh, chu c to v p xut cao. lm bch ti trng dng ti P.E kch thc 22x 36cm. b/ Nguyn liu: dng 1 trong s cc nguyn liu rm, r, tru, mt ca, ci bp. Bp, cm, tt , mi ,kh, khng m, khng mc. c/ Mi trng: Rm, r 1kg, cm 50g, bp 150g, nc vi 1%. Rm r ct ngn 2-3cm phi tht kh, trc khi lm meo ngm nc vi 1%, khong 2 gi vt ra ro nc n khi m. Bp,cm trn nc vi m trc khong 2 gi. Trn u cc nguyn liu, dm p cho mm, xong cho vo chai hay bch. d/ Kh trng: Hp kh trng chai meo v meo bch PP 1,5 atm/1 gi. Hp kh trng bch trng PE 90 100 oC trong 4 6 gi. e/Cy meo: Trong iu kin v trng, ly 1 cy meo b cho vo bch mi trng hay chai mi trng hp. f/ meo: Nm rm, 30 350C, 6 ngy y v s dng 6 10 ngy. g/Chn meo: Chm sc meo thng xuyn loi b meo nhim ngay.

T nm trn mi trng cp 3 ( nh: Xun ng)