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vn I HC THI NGUYN
KHOA KHOA HC T NHIN V X HI B MN SINH HC
TI SINH VIN NGHIN CU KHOA HC
NGHIN CU A DNG CC GING CH TRNG THI NGUYN BNG K THUT SINH HC PHN T
Gio vin hng dn: ThS. Hong Th Thu Yn Sinh vin thc hin: Nguyn Th Thu Phng
Ni dung bo co
M u Tng quan ti liu i tng v phng php nghin cu Kt qu nghin cu v tho lun Kt lun v kin ngh
M U
Thu mu l ch. Tch chit DNA tng s. o quang ph DNA Nghin cu tnh a hnh ca genome ch bng k thut RAPD. Phn tch sn phm RAPD bng phn mm sinh hc phn t.
1. Khi qut v cy ch 1.1. Ngun gc ca cy ch Vn Nam-Trung Quc. Vng Atxam thuc n Vit Nam.
Hn kn
Song t dip
Angiospermae
Dicotylleonae
Ch
Theales
Ch
Theaceae
Chi
Ch
Camellia
Loi
Camellia
sinensis
1.1.2. c im sinh hc ca cy ch
L ch l loi l n, nguyn, c h gn l rt r, c
nhiu hnh dng khc nhau: thun, mi mc, van,
trng g v gn trn.
Sn xut
Hettich Germany Germany
T lnh My in di
My o quang ph My chy PCR My soi DNA My chp nh Gel - Doc B n nhit T - 200C L vi sng
Janpan USA
Phermo Electriction Eppendorf - USA Wealtea USA Applied BioSciences Jeiotech Italy Shorp
Ti liu chia s cu 2.3. Quy trnh nghin ti: wWw.SinhHoc.edu.vn Nguyn liu l ch Tch chit v thu nhn genome tng s in di kim tra kt qu Kim tra tinh sch ca DNA bng my quang ph
Phng php tch chit DNA tng s Phng php o quang ph trn my quang ph t ghi Phng php in di Phng php RAPD Phng php x l s liu bng phn mm NTSYS 2.0
KT QU V THO LUN
3.1. Kt qu tch chit DNA tng s
1 2 3 4 5 6 7 8
1. LDP2 2. PH1 3. TDLN 4. Iakatamidori 5. Tri777 6. Keo Tam Tch 7. tri 2024 8. PT 95
Ti liu chia s ti: wWw.SinhHoc.edu.vn 3.2. Kim tra tinh sch ca DNA bng my quang ph
A = OD260nm/OD280nm
Ging LDP2
A 1.589
PH1
1.981
Tri2024
1.752
PT95
1.239
KTT
1.788
Ti liu chia s ti: wWw.SinhHoc.edu.vn 3.3. Kt qu phn tch a hnh bng k thut RAPD
+ Mi RA32
1. LDP2
2. PH1
3. TDLN 4. Iakatamidori 5. Tri777 6. Keo Tam tch 7. tri 2024 8. PT 95
1.LDP2 2. PH1
3. TDLN
4. Iakatamidori 5. Tri777
Bng 3: BngTi liu chia son wWw.SinhHoc.edu.vn thng k phn ti: DNA c nhn bn ngu nhin trong phn ng RAPD vi 2 mi ngu nhin ca 8 ging ch M G RA32 1,5 (kb) 1,25 1,0 8,5 8,0 0,7 0,64 0,6 0,5 0 1 1 1 1 0 1 1 1 0 1 1 1 1 0 1 1 1 0 0 0 1 1 1 1 0 1 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 1 1 0 1 1 1 1 0 1 1 1 1 0 1 0 0 1 0 0 0 1 2 3 4 5 6 7 8
0,2
Tng
0
7
0
7
0
5
0
3
0
1
0
4
0
7
1
4
0,5
0,3
0
0
0
0
0
0
1
0
0
1
0
0
1
1
1
1
0,2
0,18 Tng
0
1 6
0
0 3
1
0 5
0
0 4
0
0 6
0
0 4
1
0 6
1
1 9
RA32 RA40
7 9
7 7
100% 78%
Tng s phn on DNA ngu nhin l 16 v c kch thc t 0,18 1.5 kb. Trong c 14 phn on DNA a hnh chim 87,5%, 2 phn on DNA khng a hnh chim 12,5%.
3.4. Mi quan h di truyn gia cc ging ch trng Thi Ti liu chia s ti: wWw.SinhHoc.edu.vn Nguyn da trn phn tch RAPD * Kt qu h s tng ng di truyn gia cc ging ch
1 1.000 0.900 0.650 0.600 0.500 0.750 0.700 0.300 1.000 0.750 0.600 0.500 0.850 0.700 0.300 1.000 0.550 0.550 0.800 0.550 0.450 1.000 0.700 0.750 0.700 0.300 1.000 0.650 0.500 0.500 1.000 0.550 0.350 1.000 0.300 1.000 2 3 4 5 6 7 8 1.LDP2 2. PH1 3. TDLN 4. Iakatamidori 5. Tri777 6. Keo Tam tch 7. Tri 2024 8. PT 95
7. tri 2024
8. PT 95
Kt lun
1. tch chit c DNA ca 8 ging ch vi hm lng DNA kh cao. 2. Kt qu nhn c sau khi nghin cu tnh a hnh DNA c nhn bn ngu nhin chng t s sai khc nhau trong cu trc h gen ca cc ging ch. 3. Khi s dng 2 mi ngu nhin cho thy c 2 mi u biu hin tnh a hnh vi t l cc phn on dao ng t 14,3% - 90%. 4. H s di truyn gia cc ging dao ng t 0,3 0,9
Kin ngh
1. S lng mi ngu nhin s dng trong nghin cu a hnh cc ging ch cn c nhiu hn. 2. Cn kt hp nhiu phng php sinh hc phn t khc: SSR (Simple sequence region), RFLP, xc nh trnh t DNA cng vi k thut RAPD c kt qu chnh xc trong nghin cu a dng ging ch.