B CNG THNG TRNG H CNG NGHIP THC PHM TP.

HCM

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Ti: CNG NGHIN CU KHOA HC

GV: nh Nguyn Trng Ngha

Nhm 6: 1. L Minh Chc 2. Nguyn Th Ho 3. Nguyn Quc Hiu 4. Trn Th nh Ly 5. Thiu Th Bch Mai 6. L Th Ngc M 7. Nguyn Th Kiu Th

NI DUNG BO CO
I. CNG NGHIN CU KHOA HC
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1.1. nh ngha

1.2. Phn loi

1.3. Phng php xy dng 1.4. cng tng qut 1.5. cng chi tit II. CNG CHI TT C TH

I. CNG NGHIN CU KHOA HC

1.1 nh ngha

L bn m t thit k, k hoch v kinh ph ca mt ch nghin cu Hay L bn m t chi tit ca mt ch nghin cu nhm nh gi mt vn no .

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I. CNG NGHIN CU KHOA HC

1.2.Phn loi
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C 2 loi cng NCKH - cng tng qut (project concept hay concept notes): l cng th hin tng nghin cu.

- cng chi tit (research project proposal): l cng thc hin cng vic.

I. CNG NGHIN CU KHOA HC

1.3.Phng php xy dng t cu hi nghin cu L cu hi m chng ta c th tr li thng qua thu nhp v phn tch s liu. t cu hi: L ngh thut hnh thnh cu trc gi thuyt v d on kt qu Quan st
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Cu hi Gi thuyt
D on kt qu

I. CNG NGHIN CU KHOA HC

1.3.Phng php xy dng
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t gi thuyt

*Gi thuyt l mt s gii thch phng on s b v mt quan st, mt hin tng hay mt vn khoa hc m c th nghin cu bng nhng quan st su, nhng iu tra, hay php th nghim. *Hnh thnh mt gi thuyt tt s: -Tp trung c suy ngh v nh hnh nghin cu ng -Trnh mt thi gian nghin cu/ quan st 6 -Gim chi ph khi nghin cu -Gim mu vt khi nghin cu

I. CNG NGHIN CU KHOA HC

1.3.Phng php xy dng

Ngun gc ca cu hi nghin cu

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-S t m ham hiu bit, s mong mun tm hiu (vd: ti sao v nh th no m mt ng vt hay thc vt hot ng c?) -Quan st v tnh hay ngu nhin (vd: quan st tp tnh ca mt ng vt bn ngoi t nhin hay nui nht) -Quan st vi mc ch tm hiu -Cc nghin cu trc: quan st c c l xut pht t cc nghin cu trc, kho st trc thng qua vic tm hiu mt vn khc m c trong ti liu tham kho.

I. CNG NGHIN CU KHOA HC

1.4. cng tng qut
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Cu -

trc bao gm: Tn ti Ngi ch tr v ngi phi hp (cng s) Gii thiu Mc tiu Ni dung nghin cu Kt qu mong i K hoch thc hin
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I. CNG NGHIN CU KHOA HC

1.4 cng tng qut 1.4.1.Tn ti -Ngn gn, th hin c mc tiu, ni dung v kt qu k vng -T quan trng t trc, trnh t tha -Da vo quan st v gi thuyt t tn ti 1.4.2.Ngi ch tr/ phi hp -Tn ngi ch tr (ch nhim ti) -Tn ngi phi hp thc hin ti 1.4.3. Gii thiu/ t vn -Nu nhng vn chnh lin quan n ch qua lc kho ti liu c lin quan -Nu ln c nhu cu cn thit ca ti t gip ngi c hiu c ti sao phi nghin ti ny
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I. CNG NGHIN CU KHOA HC

1.4 cng tng qut 1.4.4. Mc tiu -Mc tiu tng qut (mc tiu lu di): nhng iu m ti sau khi thc hin s ng gp cho mt mc tiu ln. -Mc tiu c th (mc tiu trc mt, mc tiu ngn hn): l iu m ti s t c sau khi trin khai nghin cu -Khi vit mc tiu trnh dng nhng t ch hnh ng u cu (vd:iu tra, kho st, nh gi,) 1.4.5.Ni dung -Gip ngi c hnh dung khi lng cng vic ca ti v xem xt c ph hp vi mc tiu v tn ti hay khng. -Lit k cc ni dung m ti d kin s tin hnh, lu ch nu nhng ni dung chnh m cha cn nu c th tng th 10 nghim ca nghin cu
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I. CNG NGHIN CU KHOA HC

6.Kt qu mong i -Lit k nhng kt qu c th m ti mong i s t c (so vi mc tiu th kt qu mong i c tnh c th hn) 7.Kt qu thc hin -Nu tng qut k hoch thi gian ca ti ( thy tin v tnh kh thi). C th dng dng s trnh by, nn nu k hoch theo cc ni dung nghin cu ca ti 8.D ton kinh ph -Nu cc mc kinh ph chnh (cha cn tnh ton chi tit) c th c mu hng dn km theo 9.Ti liu tham kho -Lit k tt c cc ti liu tham kho dng vit cng

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I. CNG NGHIN CU KHOA HC

1.5 cng chi tit Cu trc cng gm 1.5.1. Tn ti 1.5.2. Ngi ch tr phi hp 1.5.3. Gii thiu +Gii thiu chung +Mc tiu v gi thuyt +Ni dung nghin cu +Kt qu mong i 1.5.4. Phng php nghin cu 1.5.5. K hoch thc hin 1.5.6. D ton kinh ph 1.5.7. Ti liu tham kho

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1.5 cng chi tit 1.5.1.Tn ti (ging nh cch t tn ti NCTQ) 1.5.2.Ngi ch tr/ phi hp -Tn ngi ch tr (ch nhim ti) -Tn ngi phi hp thc hin ti 1.5.3.Gii thiu t vn -Gii thiu chung +Gii thiu ngn gn cc vn lin quan ti ti +Ni c tnh cp thit ti sao chn ti ny -Mc tiu ca ti: +Mc tiu tng qut v mc tiu c th (nhng ti nh c th khng cn nu mc tiu tng qut). VD: nhm bit hay hiu c s nh hng ca vic s dng ha cht n sc khe c tra nui trng ao

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I. CNG NGHIN CU KHOA HC

1.5 cng chi tit 1.5.3.Gii thiu t vn
-Gi thuyt: gi thuyt l iu m ti t ra thc hin
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nghin cu nhm chng minh iu nu ra l ng hay sai chp

nhn hay bac b gi thuyt t ra VD: Cho c nhiu ln trong nm s khng nh hng n cht lng c bt sinh ra? -Ni dung nghin cu: lit k cc ni dung chnh m ti s

thc hin ch cha cn chi tit ha ni dung s nghin cu.

VD: phn tch s bin ng li sut ca ngn hng trong nm 14 2012 (Khng cn nu r l cch thu thp s liu nh th no)

I. CNG NGHIN CU KHOA HC

1.5 cng chi tit
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1.5.3.Gii thiu t vn -Kt qu mong i: Lit k cc kt qu c th m ti k vng s t c sau khi thc hin nghin cu VD: Xc nh c cc nguyn nhn gy ra s bin ng v gi mua c tra v tm s BSCL trong nm 2012 Hay Xc nh c nguyn nhn gy ra bnh trng ui tm cng xanh sau khi ti kt thc

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1.5 cng chi tit 1.5.4. Phng php nghin cu (PPNC) -L phn quan trng nht ca cng, PPNC ng s to s chnh xc v lng tin ca ngi c vi kt qu nghin cu. -PPNC phi c vit chi tit, lm sao c th c hiu v trin khai c cng vic. VD +Th nghim: m t tng th nghim nh s nghim thc, s ln lp li, mu vt, vt t ha cht, iu kin th nghim, chm sc v qun l, cch ly mu( s lng, s ln,mc chnh xc ca dng c ly mu,), ch s cng thc tnh ton,
+iu tra phng vn: S mu iu tra, cch chn mu, a im chn iu tra, chun b v th biu mu, tp hun v biu mu, 16 phng php x l s liu (ch s v cc cng thc tnh, phn mm s dng,)
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I. CNG NGHIN CU KHOA HC

I. CNG NGHIN CU KHOA HC

1.5 cng chi tit 1.5.5. K hoch thc hin -Nu r thi gian cho tng cng vic c th (k c x l s liu v vit bo co, phi nu r v chi tit hn i vi cng tng qut) -Nn dng s biu hin 1.5.6.Kinh ph -Tnh ton chi tit cho tng mc chi nh cng lao ng (lng), vt t ha cht, my mc thit b, thu mn chuyn mn, hc bng, hc ph,(c hng dn theo ngi ti tr kinh ph) 1.5.7.Ti liu tham kho -Lit k tt c ti liu tham kho (TLTK) dng vit cng

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II. CNG CHI TT C TH

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II. CNG CHI TT C TH

2.1. Tn ti
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Nghip v Biu hin gen Cry1Ac khng su thuc b cnh vy (lepidoptera) trn cy c chua (Licopersicon esculentum Mill.) 2.2. Ngi ch tr phi hp 1. TS. Dng Hoa X TT CNSH TP. HCM 2. TS. Nguyn Quc Bnh TT CNSH TP. HCM 3. TS. V Th o TT CNSH TP. HCM 4. ThS. H Th Loan TT CNSH TP. HCM 5. CN. Nguyn Th Ngc Tho TT CNSH TP. HCM

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II. CNG CHI TT C TH 2.3. Gii thiu

Gii thiu chung Vic s dng cc lai thuc tr su ny a phn c ngun gc ha hc v chng gy ra nhiu tc ng tiu cc n mi trng, sc khe con ngi v vt nui. Mt trong nhng phng n thay th cho vic s dng thuc tr su ha hc l s dng thuc tr su c ngun gc vi sinh vt v thc vt. V vy mt cch tip cn khc trong vic hn ch su bnh trn cy c chua l to cy chuyn gen mang v biu hin gen c t B. thuringiensis nh m bo v cy khi s ph hoi trong sut qu trnh sinh trng. Do chng ti tin hnh nghin cu ti: Biu hin gen Cry1Ac khng su thuc b cnh vy (lepidoptera) 20 trn cy c chua (Licopersicon esculentum Mill)
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II. CNG CHI TT C TH 2.3. Gii thiu

Mc tiu tng qut: Nghin cu to mt s ging c chua (Licopersicon esculentum Mill) mang gen Cry1Ac c kh nng khng su thuc b cnh vy (lepidoptera) Mc tiu c th: Mc tiu nm 2011 - V mu ht mt s ging c chua lm ngun vt liu chuyn gen. - Gy nhim vi khun Agrobacterium tumefaciens c cha plasmid mang gen Cry1Ac, gen bar v gen gus vo cc b phn khc nhau trn cy c chua trong iu kin in vitro. Mc tiu nm 2012 Sng lc v ti sinh cc dng c chua chuyn gen thnh cng. 21 nh gi kh nng khng su thuc b cnh vy lepidoptera ca cy c chua chuyn gen thu nhn c
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II. CNG CHI TT C TH 2.3. Gii thiu

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Ni dung nghin cu
Tng quan v cy c chua Su, bnh hi trn cy c chua Tnh hnh sn xut c chua trn th gii Tnh hnh nghin cu chn to ging c chua Nghin cu chuyn gen Bt trn cy c chua Gii thiu c t tr su ca vi khun Bacillus Thuringinesis Gii thiu vi khun Bacillus thuringiensis Phn loi protein Cry C ch hot ng ca c t Gii thiu vi khun Agrobacterium

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II. CNG CHI TT C TH 2.3 Gii thiu Kt qu mong i Cn c trn cc ni dung thc hin ca ti, chng ti d kin kt qu nh sau: Thu nhn c ngun vt liu chuyn gen khng su thuc b cnh vy (lepidoptera) bng k thut in vitro. Hon thin quy trnh chuyn gen cy c chua khng su bng vi khun Agrobacterium tumeficient. Tm c mi trng nui cy cy c chua chuyn gen ti u. Thu c cc dng c chua chuyn gen c kh nng 23 khng su thuc b cnh vy (lepidoptera)
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II. CNG CHI TT C TH 2.4 Phng php nghin cu

To ngun vt liu chuyn gen. Gy nhim vi khun Agrobacterium tumefaciens c cha plasmid mang gen Cry1Ac, gen bar v gen gus vo cc b phn khc nhau trn cy c chua trong iu kin in vitro. Sng lc v ti sinh cy c chua chuyn gen thnh cng. nh gi kh nng khng su thuc b cnh vy (lepidoptera) ca cy c chua chuyn gen.
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2.5. K hoch thc hin

Cc ni dung, cng vic thc hin ch TT yu 1 To ngun vt liu chuyn gen 2 3 4 Xy dng quy trnh chuyn gen khng su trn cy c chua . nh gi cc tnh trng di truyn ca cc dng c chua chuyn gen Ti sinh cy chuyn gen trong iu kin in vitro. Thu nhn v kim tra kh nng khng su ca cy chuyn gen trong iu kin in vitro nh gi kh nng khng ca cy chuyn gen Thi gian (Bt u-Kt thc)
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2 thng (05/2011) 3 thng (06/2011) 6 thng (07/2011-02/2012) 6 thng (03/2012 - 08/2012)

3 thng (09/2012 - 11/2012)

6 thng (12/2012-04/2013)
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7 Vit bo co nghim thu ti

1 thng 05/2013

II. CNG CHI TT C TH 2.6. D ton kinh ph

Thi gian thc hin ti: 2 nm (5/2011-5/2013) Kinh ph d kin: 210.000.000 Cng lao ng: 100.000.000 Nguyn vt liu: 400.000.000 Chi ph khc: 150.000.000

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II. CNG CHI TT C TH 7. Ti liu tham kho

TI LIU TING VIT 1. T Thu Cc, 2007. Gio trnh cy rau. Nh xut bn nng nghip, H Ni. 2. Trn Quc Dung, 2006. Cng ngh chuyn gen ng vt, thc vt. Nh xut bn Hu. 3. Trnh nh t, 2006. Cng ngh sinh hc, Tp 4, Cng ngh di truyn. Nh xut bn gio dc, H Ni. 4. Nguyn c Lng, 2006. Cng ngh t bo. Nh xut bn i hc quc gia tp. H Ch Minh. TI LIU TING ANH 5. Hong-Xia Zhang v Eduardo Blumwald, 2001. Transgenic salt-tolerant tomato plants accumulate salt in folige but not in fruit, Nature biotechnology, Vol.19. 765768. 6. Manoj K Sharma, 2009. A simple and efficient Agrobacterium-mediated procedure for transformation of tomato. Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, 27 India.
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