Mathematics Exercises in Biotechnology Edited by Mary Ann Hovis Robert L.. Kimball John C. Peterson February 11, 2005 NSF Award #: DUE 0003065Contents Preface vi Cross-Reference Table, by Chapter, of Mathematics and Biotechnology Areas viii I Concentration/Dilution 1 1 Concentration of Egg White Lysozyme 2 2 Restriction Digestion of DNA 4 3. Using a Standard Graph to Determine the Concentration of an Unknown Solu- tion 6 4 Determining the Number of Bacteria in a Culture 8 5. Serial Dilutions 10 6 Dilutions of Stock Liquid Solutions 12 7 Defining and Distinguishing Between Concentration and Amount of Solute 14 8 Absorbance and Transmittance in a Spectrophotometer 17 9 Creatine Kinase and Tissue Injury 19 10 PCR Laboratory Using Custom Primers 21 11 Cytotoxicity Testing of Anti-Cancer Drugs 23 12 Soft agar cloning 25 ‘Work on this project wae partially funded by the National Science Foundation. NSF Award 4: DUE 00 0306513 Cell Culture Medium II Solution Preparation 14 Primer Preparation 15 Buffer Preparation 16 Percent Solution Conversion 17 Preparation of % solutions 27 29 30 32 34 36 18 Calculating How Much Vitamin to Add to Nutrient Broth in a Company Work- place 19 Gel electrophoresis reagent 38 40 20 One Hundred Percent Correct Each Time: Preparing Solutions for the Biotech- nology Industry LL Serial Dilution 21 Saving the Cell Culture 22 Test for Endotoxin: A Serial Dilution Problem IV Calibration 23 Monthly Check on Pipetter Accuracy and Precision V_ Molarity 24 Salinity Difference 25 Reagent Preparation Using Weight to Volume 26 Purification of Lactate Dehydrogenase (LDH) ‘Work on this project wae partially funded by the National Science Foundation, 42 46 48 52 54 55 37 NSF Award 4: DUE 00 03065