Nhm I.

3
Hunh Th Hng Lin

0815355

L Th M Tho

0815649
Bi bo co:

NH GI S BIN IU BCH CU KHI GY P NG MIN DCH

V GHP T BO UNG TH V PH N VIT NAM
I.

Mc ch

Tm hiu v thc hnh

K thut tim thuc.

K thut kho st s bin iu bch cu.

K thut ghp t bo d loi.

nh gi s tc ng ca cc cht c ch min dch v ghp d loi ln chut nht trng.

II.

Gii thch kt qu chut ca nhm I.3

a/ Qu trnh x l thuc vo cc ngy N0, N1, N5, N7 v N12 ca nhm I.3

-

Ngy N0: m bch cu tng, tim dung dch sinh l.

Ngy N1: Tim thuc xoang bng, c i. Tim 10ml (ng tim 100 IU) thuc Busulfan
vo xoang bng chut. Tim 0,4ml (ng tim 40IU) thuc Cyclophosphamid vo c i
chut.

Ngy N5: Tim thuc xoang bng, c i ( tng t N1).

Ngy N7: m bch cu tng, khng ghp t bo ung th v.

Ngy N12: Khng x l thuc. m bch cu tng . Kim tra chut.

b/ Ktqu
-

S bch cu tng qua cc ngy N0, N7, N12

Ngy
Bch cu tng

N0

N7
10900

N12
400

3100

Chut khng c khi u.

c/ Gii thch kt qu:

-

Ngy N0 m s lng bch cu tng chut bnh thng c 10900 t bo/l s lng
bch cu ca chut mc bnh thng.

Tim thuc ngy N1, N5: Tim 10ml (ng tim 100 IU) thuc Busulfan vo xoang bng

chut. Tim 0.4ml(ng tim 40 IU) thuc Cyclophosphamid vo c i. Cch chn v tr

tim nh trn: V Busulfal ha tan trong dung mi DMSO cc c chch xoang bng phn
tn t t gim bt tc ng cho chut; v Busulfal nng v mnh hn Cyclophosphamid
nn chch mt mi kt hp vi Cyclophosphamid. Nu ch tim Busulfal m khng tim
Cyclophosphamid d lm chut cht hoc nu ch chch Cyclophosphamid t gy p ng
min dch.
Busulfan v Cyclophosphamide l cc cht alkyl ha gn vi guanine ca DNA to s bt

cho ca chui ri gy ra s thoi ha ca chng, cc t bo phn chia s b ngng thi k

tin phn bo. Cc cht ny c tc dng mnh trn t bo min dch, kim hm t bo min
dch nn n ngy N7 ta m s lng bch cu tng chut thy gim ng k t 10900 t
bo/l ngy N0 gim xung ch cn 400 t bo/l ngy N7 sau khi tim thuc.
Ngy N7 khng tim t bo ung th nn s lng bch cu tng tng khng ng k ngy

N12 l 3100 t bo/l, do ta khng lin tc tim thuc gy suy gim min dch cho chut nn
lng bch cu tng khng gim xung m tng ln, s lng bch cu tng y l do bch
cu mi c sn sinh ra. C ch dn phc hi ca h min dch.
d/ Kt lun
S bch cu tng ca nhm I.3 tng gim qua cc ngy N0, N7, N12 l hp l .
III.

Gii thch kt qu chung ca c lp

1. So snh cc ngy th nghim trong l 1(so ngy N0 vi N7, N0 vi N12 v N7 vi N12)

a/ Bng s liu so snh cc ngy th nghim trong l 1
Ngy

N0

N7

N12

L 1 (khng SGMD,

7030 913

8590 1528

10683 1992

khng ghp tb)

b/ Biu

Biu nh gi s bin iu bch cu

khi gy p ng min dch v ghp t bo
ung th

t bo/l

15000

10000

L 1 (khng SGMD,
khng ghp tb)

5000

0
N0

N7

N12

c/ Gii thch
So snh ngy N0 vi N7, |t stat| < |t Critical two -tail| ( 0,730149 <2,776445) nn c th kt
lun s lng bch cu tng ngy N0 v N7 l tng ng nhau. So snh ngy N0 vi
N12, |t stat| < |t Critical two -tail| (1,429491 < 2,77644), So snh ngy N7 vi N12, |t stat| <
|t Critical two -tail| (2,73398 < 2,776445) s lng bch cu tng ngy N0 v N12
tng ng nhau. Do thuc c tim ngy N1, N5 l dung dch sinh l PBS nn khng
gy p ng min dch cho chut.
d/ Kt lun
Ngy

N0

N7

N12

L 1 (khng

7030 913 A

8590 1528A

10683 1992A

SGMD, khng ghp

tb)
S bin ng s lng bch cu ngy N0, N7 v N12 tng ng nhau, nn s liu ny hp
l.
2. So snh cc l th nghim vi nhau trong ngy N0 (l 1 vi l 2, l 1 vi l 3, l 1 vi l 4,
l 2 vi l 3, l 2 vi l 4, l 3 vi l 4)
a/ Bng s liu so snh cc l th nghim vi nhau trong ngy N0

L 1

L 2

L 3

L 4

Bch cu tng

7030 913

5520 1297

6505 1104

8400 1488

ngy N0
b/ Biu

Biu nh gi s bin iu bch cu

khi gy p ng min dch v ghp t
bo ung th
10000

t bo/l

8000
6000
4000
ngy n0

2000
0
l 1

l2

l3

l4

c/ Gii thch

Gi tr p-value two - tail

So snh vi 0,05

Kt lun

L 1 V L 2

0,3689

>0,05

Khng c s khc bit

L 1 V L 3

0,7296

>0,05

Khng c s khc bit

L 1 V L 4

0,4383

>0,05

Khng c s khc bit

L 2 V L 3

0,5745

>0,05

Khng c s khc bit

L 2 V L 4

0,1866

>0,05

Khng c s khc bit

L 3 V L 4

0,3265

>0,05

Khng c s khc bit

S bin ng s lng bch cu ca cc l trong ngy N0: Gi tr p-value two tail khi so snh
cc l vi nhau u ln hn 0,05 s lng bch cu tng cc l 1, l 2, l 3, l 4 u
tng ng nhau. Thi im No khi m s bch cu tng cc l l lc chut bnh thng

cha b tim thuc hay ghp t bo ung th nn bin ng s lng bch cu tng cc l
gn nh l ging nhau.
-

S liu bch cu tng khc nhau mi l: l do h min dch ca cc con chut cc l khc
nhau th khng ging nhau, do ngi m bch cu c s nhm ln, do qu trnh thao tc ly
mu v x l bung m bch cu, thi gian bung m ngoi khng kh lu lm kh mu
trc khi em quan st

d/ Kt lun
L

L 1

L 2

L 3

L 4

Bch cu

7030 913 A

5520 1297A

6505 1104A

8400 1488A

tng ngy
N0
S lng bch cu ca cc l 1, l 2, l 3, l 4 ngy N0 u tng ng nhau. S liu m
bch cu tng cc l ny hp l
3. Biu nh gi s bin iu bch cu khi gy p ng min dch v ghp t bo ung
th qua cc l (s liu c lp)
a/ Bng s liu bch cu tng ca c lp
Ngy

N0

L 1 (khng SGMD, 7030913 ABM

N7

N12

85901528 AM

106831992 AMP

76201935 ABM

134303009 ABM

841261 BN

2368361 BCN

2200 1058 BN

101074211 CP

khng ghp tb)

L 2 (khng SGMD, 55201297 ACM
c ghp tb)
L 3 (c SGMD, 65051104 ACM
khng ghp tb)
L 4 (c SGMD, c 8400 1488 ACM
ghp tb)
(vi A, B, C k hiu cho s khc nhau ca cc l so snh khc thi im. M, P k hiu cho s
khc nhau ca cc l so snh cng thi im).

Biu nh gi s bin iu bch cu khi

gy p ng min dch v ghp t bo ung th
16000
14000
12000
L 1 (khng SGMD,
khng ghp tb)

t bo/l

10000
8000

L 2 (khng SGMD, c
ghp tb)

6000

L 3 (c SGMD, khng
ghp tb)

4000

L 4 (c SGMD, c ghp
tb)

2000
0
N0

N7

N12

Kt lun chung qua cc l

-

L 1 khng suy gim min dch khng ghp t bo s lng bch cu tng thay i
khng ng k.

L 2 khng suy gim min dch c ghp t bo s lng bch cu tng tng ng k
ngy N12.

L 3 c gy suy gim min dch, khng ghp t bo s lng bch cu tng gim mnh
ngy N7 v tng t ngy N12.

L 4 c gy suy gim min dch, ghp t bo s lng bch cu tng gim ng k

ngy N7 v tng nhiu ngy N12.