THC TP NG DNG TIN HC TRONG SINH HC

(Dnh cho sinh vin 50CNSH) Bi 1: Khai thc c s d liu trn mng Internet (NCBI)
1. Yu cu: Nm c nhng cng c chnh trong c s d liu NCBI nh tm kim phn mm tin sinh, tm kim sch bo, tm kim trnh t, tm kim cu trc proteinT , lin h n nhng cng c tng t trn cc c s d liu khc nh EMBL, DDBJ 2. Ni dung thc hnh: a. Tm kim cc bi bo, cc cng trnh cng b lin quan bacteriocin, bacteriocin shrimp C bao nhiu kt qu? Chn 1 kt qu, ti v v phn tch ni dung? So snh kt qu khi tm kim trn EMBL?

b. Tm kim trnh t gene lin quan lung cancer C bao nhiu kt qu? Chn mt kt qu v phn tch ni dung?

c. Tm kim trnh t ca x khun streptomyces C bao nhiu kt qu tm c? Chn 10 kt qu c trnh t ln hn 700bp s dng cho cc phn tch tip theo 3. Thc hnh v bo co kt qu?

Bi 2: Tm kim trnh t tng ng: BLAST

1. Yu cu: tm kim c trnh t (DNA v Protein) tng ng ph hp bng cng c BLAST 2. Ni dung thc hnh: a. Tm kim trnh t DNA v trnh t Protein lin quan H5N1 C bao nhiu kt qu tm c? Chn mt trnh t ty (ly FASTA)

b. Tin hnh Blastn, Blastp v Blastx? So snh cc kt qu tm c? 3. Thc tp v bo co kt qu?

Bi 3: Thao tc vi trnh t DNA th

1. Yu cu: X l c s liu v trnh t DNA thu c t my gii trnh t sequencing 2. Phn mn s dng: Bioedit; NCBI; BLAST 3. Ni dung thc hnh: a. Phn tch, chn la kt qu sequencing? b. Ni 2 trnh t c gii trnh t bng 2 mi (overlap) c. Blast cc trnh t thu c? 4. Thc tp v bo co kt qu?

Bi 4: Xy dng cy phn loi

1. Yu cu: Nm c cc bc xy dng cy pht sinh loi trong phn tch a dng sinh hc 2. Phn mm s dng: BLAST, ClustalX v Njplot, TreeView 3. Ni dung thc hnh: a. Tm kim trnh t tng ng bng BLAST b. S dng phn mm ClustalX dng cy phn loi c. Hin th kt qu bng phn mm Njplot hoc TreeView 4. Thc tp v bo co kt qu?

Bi 4: Thit k primer cho PCR v thit lp bn enzyme ct gii hn

1. Yu cu: S dng cc phn mm thng dng trc tuyn hoc khng trc tuyn trong thit k mi cho phn ng PCR v thit k bn enzyme ct gii hn. 2. Ni dung thc hnh: a. S dng phn mm DNA club trong thit k mi v bn enzyme ct gii hn? b. S dng cc phn mm online khc c hc c. So snh cc kt qu thu c t cc cch trn? 3. Thc tp v bo co kt qu:

Bi 5: trnh trnh t ln c s d liu v hin th cu trc protein

1. Yu cu: - Nm c cc bc c bn trong vic trnh mt trnh t sinh hc ln c s d liu. - Bit cch hin th cu trc khng gian ca phn t protein 2. Ni dung thc hnh: a. S dng phn mm Sequin? b. S dng phn mm Cn3D? 3. Thc tp v bo co kt qu?

Bi 6: Cu hi Tho lun v kim tra

Lu : Trn y ch l ni dung cc bc thc tp. Mt s yu cu c th s c giao khi cc bn thc tp trc tip trn lp!

Chc cc bn hc tt!