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Sinh vin :
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30660073
30660246
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30600024
Contents
I. Gii thiu:.....................................................................................................................................4
II. C s chn ti:........................................................................................................................4
a. Phng php ny c u im: .................................................................................................5
b. Nhc im: ...........................................................................................................................5
III. Tng quan:.................................................................................................................................5
C 6 loi enzyme c xp vo 2 nhm: ...................................................................................5
IV. Nm mc Aspergillus oryzae:...................................................................................................5
V. Quy trnh cng ngh:...................................................................................................................6
1. Bo qun ging:.......................................................................................................................6
b. Nguyn liu:.............................................................................................................................6
c. Quy trnh:.................................................................................................................................7
d. Thuyt minh quy trnh:............................................................................................................9
e. My mc, thit b, ho cht:...................................................................................................10
VI. ng dng ................................................................................................................................10
1. ng dng amylase trong sn xut bia....................................................................................10
3. ng dng amylase trong ch bin thc phm gia sc...........................................................11
4. ng dng enzym amylase trong cng nghip dt
....................................................................................................................................................12
VII. CC NH HNG CA SN XUT N MI TRNG V X HI:.....................15
1. nh hng mi trng:.........................................................................................................15
b. nh hng x hi:.................................................................................................................16
i. Tch cc:.............................................................................................................................16
ii. Tiu cc:............................................................................................................................16
VIII. Kt lun.................................................................................................................................17
Tm li, c th ni rng, vic nghin cu ng dng cc ch phm enzyme ngy cng c
ch trng cc lnh vc khc nhau. Trong 20 nm cui th k XX v cc nm u ca th
k XXI cc enzyme khc nhau c ng dng. Vit Nam bc u c nhiu nghin
cu ng dng cc enzyme trong ch bin nng sn, thc phm, nht l trong lnh vc sn
xut bia, ru, ch bin tinh bt (Vin cng nghip thc phm, Vin cng ngh sinh hc
cng ngh thc phm, i hc Bch khoa H Ni). Vic nghin cu cc enzyme phc v
nng nghip, cng nghip cng c quan tm v c cc kt qu ng khch l. V d, ch
phm enzyme mi ra i phc v nng nghip E2001 c tc dng tng ph nhiu t,
tng nng sut cy trng. c cc nghin cu ng dng protease trong sn xut ru bia,
rt ngn thi k ln men cng nh sn xut nc mm ngn ngy bng cng ngh enzyme
protease.
Ti liu tham kho:
1. Nguyn c Lng v mt s tc gi, Cng ngh enzym, NXB i hc quc gia
Tp.HCM, 2004
2. Nguyn c Lng, Vi sinh vt cng nghip ( Cng ngh vi sinh Tp 2 NXB i hc
quc gia Tp.HCM, 2006
3. www.bio-link.org/sharing_day/fungalamylase.pdf...........................................................17
I.
Gii thiu:
II.
C s chn ti:
III.
Tng quan:
Enzyme amylase:
Amylase l mt h enzyme rt ph bin trong th gii sinh vt . Cc enzyme ny
thuc nhm enzyme thy phn, xc tc phn gii lin kt ni phn t trong nhm
polysaccharide vi s tham gia ca nc:
R.R. + H - OH
RH + ROH
C 6 loi enzyme c xp vo 2 nhm:
Endoamylase ( enzyme ni bo ) gm:
-1,4-glucano hydrolase
-1,6 glucano hydrolase, nhm nh ny gm isoamylase (EC 3.2.1.68) hay
pullulanase (EC 3.2.1.41).
Exoamylase ( enzyme ngoi bo )gm:
-amylase (EC 3.2.1.2)
Amyloglucosidase (glucoamylase) (EC 3.2.1.3).
C cht tc dng ca amylase l tinh bt v glycogen.
IV.
Nm mc Aspergillus oryzae:
V.
Chng mc ging
chng Aspergyllus oryzae m s VTCC-F-048 mua ca Bo tng ging chun vi
sinh vt thuc Vin Vi sinh vt v Cng ngh sinh hc
a ch: Nh E2 - 144 ng Xun Thu, Cu Giy, H Ni
in thoi: (04) 7547407; Fax: (04) 7547407
Website: http://www.biotechvnu.edu.vn
1. Bo qun ging:
Cho ct vo ng nghim, tit trng trong autoclave 130oC trong 30 pht.
Trn bo t vo ct.
Sy chn khng < 40oC ( m 5%).
Dng parafin rn un chy v ln nt bng.
Thi gian bo qun: 1 nm hoc hn.
b. Nguyn liu:
-Cm go: cha khong 20% tinh bt, 1015% cht bo, 10-14% protein, 816% cellulose, cc cht ho tan khng cha nit 37-59%.
Yu cu i vi quy trnh l khng c cha tinh bt di 20-30%. S dng cm
tt, cm mi khng c v chua hay ng, khng hi mi mc. m ca cm
khng qu 15%, tp cht c khng qu 0,05%.
Cc ph phm thm vo tng thong kh cho mi trng nui cy: tru, mt
ca
c. Quy trnh:
Nguyn liu
X l
Thanh trng
Lm ngui
Ging
VSV
Phi trn
Nui cy
Thu nhn ch phm
enzyme th
Sy
Nghin mn
Trch ly
Lc
Thnh
phm
Kt ta enzyme
Bao gi
Sc k
Sy
VI.
ng dng
Hin nay, vic sn xut ch phm enzyme cc loi v ang pht trin mnh m
trn qui m cng nghip. Thc t c hng nghn ch phm enzyme bn trn th
trng th gii, cc ch phm ny c khai thc v tinh ch c mc tinh
khit theo tiu chun cng nghip v ng dng. Ch phm enzyme khng ch c
ng dng trong y hc m cn c ng dng trong nhiu lnh vc cng nghip
khc nhau, trong nng nghip, trong ha hc " ngha ca vic s dng enzyme
trong cc lnh vc thc t khng km so vi ngha ca vic s dng nng lng
nguyn t".
Theo thi gian, enzym cng nghip ngy cng c ng dng vo nhiu lnh vc
khc nhau, trong nhng enzym ng dng nhiu nht l protease, cellulose,
ligase, amylase, v mt s enzym c bit khc thu c rt nhiu li nhun
t ngnh ny. Di y l mt vi ng dng thc t:
1. ng dng amylase trong sn xut bia
Trong cng ngh sn xut bia truyn thng, cc nc phng Ty ch yu s dng
enzym amylase ca malt thy phn tinh bt trong malt, sau n giai on
ru ha bi nm men Saccharomyces sp. C s khoa hc ca vic s dng
amylase ca malt ch, khi i mch chuyn t trng thi ht sang trng thi ny
mm (malt), enzym amylase s c tng hp v khi enzym ny s thy phn
tinh bt c trong ht to ra nng lng v vt cht cho s to thnh mm. Nh vy
vic ng ha tinh bt trong ht nh enzym ca chnh n. Khi ht ch tng hp
ra lng enzym amylase va phn hy lng tinh bt c trong ht. Nh th
cn rt nhiu mm i mch sn xut bia qui m ln, dn n chi ph cao cho
sn xut v sn phm. khc phc iu ny, trong qu trnh ln men to bia th
nh sn xut khng s dng hon tan 100% nguyn liu l malt i mch m c
s pha trn theo mt cng thc no thay th malt v cn b sung ngun tinh
bt cho qu trnh ln men. L do l mt phn to hng v cho bia, mu sc,
cn ph hp cho ngi tiu dng v mt phn l lm gim gi thnh cho sn phm
lng t ngun tinh bt, ngi ta thng cho thm enzym amylase vo. Enzym
amylase s tham gia phn gii tinh bt to thnh ng, gip cho qu trnh chuyn
ha tinh bt tt hn.
Bng bo co ti chnh
Vn iu l:3 t
Vn lu ng: 2 t
1.
2.
3.
Ngun: Hanoitrans
Chn loi xe Huyndai Mitsumi 2,5tn thu trong 1 nm, gi 1.300.000/ xe/ ngy
( vn chuyn cm go)
4. Gi cc ph phm thm vo: 1.000.000 ng/ tn, 1 ngy dng 0,5tn=
500.000 ng/ ngy
5.
Gi thit b, gm (n v: VN):
my
Thit b
nghin( cng ty Phan
Hng Long):
120.000.000/ my
My lc tip tuyn
( cng ty TNHH
TBKH Vit Anh):
200.000.000/ my
6. Tin mua gel: 1kg gel gi 2.000.000, 1 ngy sn xut dng 10kg =>
20.000.000
7.
VII.
1. nh hng mi trng:
- Ngun nc thi gy nh hng n mi trng nc chung quanh ni sn
xut: mi, nhit , pH v cc yu t khc trong nc thi lm ri lon
n h VSV trong nc ti vng . Cc ch tin BOD, COD, coliform s c
s thay i.
- Ngun c cht sau khi c VSV s dng xong, tr thnh cht thi rn c
kh nng tr thnh mi trng cho cc VSV c hi pht trin, v mi hng
kh chu s gy nh hng khng tt n sc khe v mi trng sng ca
ngi dn xung quanh.
- V sn xut theo quy m cng nghip nn vic thi cc loi kh thi l iu
khng trnh khi. Cc loi kh thi ny c th gy nh hng n bu khng
kh sng, gp phn lm bin i kh hu v thi tit
b. nh hng x hi:
i.
Tch cc:
Tiu cc:
ngun nc b nhim
VIII.
Kt lun