LU N V N TH C S HA H C

NGHIN C U T NG H P CH T HO T NG B M T SINH H C M C BASA

H C VIN: NGUY N HONG LY MSHV: 090813 L P: HA H U C K15

ANION T
GVHD: TS. BI TH B U HU B MN HA KHOA KHOA H C T NHIN IH C C N TH

C n Th 09/2010

N I DUNG BO CO
1. T 2. N 3. K 4. K ng quan i dung v ph ng php nghin c u t qu v th o lu n t lu n v ki n ngh

1. T ng quan
Ch t ho t ng b m t (CH BM) t khi ra i gi m t vai tr kh quan tr ng trong cu c s ng.

M ph m Ch t t y r a (40%) Th c ph m

S n

D t may

c ph m

Qu trnh pht tri n c a CH BM

http://www.rsc.org/chemistryworld/issues/2003/July/amphiphiles.asp

 CH BM c t ng h p t ngu n nguyn li u d u m b phn h y sinh h c ch m. Ngu n ti nguyn thin nhin c n ki t, v n nhi m mi tr ng v i nhi u ha ch t c h i t n t i dai d ng, Nghin c u t ng h p cc CH BM thn thi n v i mi tr ng t nh ng ngu n nguyn li u ti t o: d u th c v t v m ng v t.

 M in m basa.

BSCL th i ra kho ng 30.000 t n m c

M c ny c dng s n xu t: d u th c ph m, m bi tr n, d u biodiesel, th c n ch n nui, T n d ng cc g c hydrocarbon c a cc acid bo trong m c s n xu t CH BM l h ng i kh m i Vi t Nam. Nghin c u t ng h p CH BM sinh h c anion t m c basa

Ch t ho t

ng b m t l g?

L nh ng ch t c kh n ng h p ph trn b m t phn chia pha, c tc d ng lm gi m n ng l ng t do v s c c ng b m t phn chia pha (khl ng, l ngl ng, l ngr n). C u t o:

Phn lo i CH BM
D a vo b n ch t c a nhm a n c, CH BM c phn thnh 4 lo i bao g m: CH BM anion, CH BM cation, CH BM khng ion v CH BM l ng tnh. CH BM khng ion CH BM anion CH BM cation CH BM l ng tnh

V d :
CH BM anion CH BM cation CH BM l ng tnh

CH BM khng ion

CH BM sinh h c l g?
Thu t ng CH BM sinh h c (biosurfactants) c th hi u l: + CH BM c s n xu t b i vi sinh v t. + CH BM thn thi n v i mi tr ng c s n xu t t ngu n nguyn li u c kh n ng ti t o nh : d u th c v t, m ng v t. Nh ng h p ch t ny c kh n ng ho t t ng t nh CH BM thng th ng. ng b m t

u i m CH BM sinh h c
i u ch t ngu n nguyn li u ti t o: d u th c v t, m ng v t. c tnh th p, kh n ng b vi khu n phn h y cao, thn thi n mi tr ng, ng d ng trong nhi u l nh v c: b o v mi tr ng, nng nghi p, ch m sc y t , ch bi n th c ph m, d c ph m,

Ph h p v i chu trnh carbon.

Tnh hnh nghin c u CH BM sinh h c

Ngoi n c: N m 2002, Piispanen v Peter (
COOH Acid oleic MeOH, H2SO4, to OCH3 O HCOOH/H2O2 OCH3 O PEG OCH3 HO O O OH O n O

c)

Tnh hnh nghin c u CH BM sinh h c

Trong n c: N m 2004, Ph m Thnh Qun ( H Bch Khoa tp HCM) t ng h p CH BM khng ion APG (Alkyl Polyglucosides).

C basa (Pangasius bocourti)

L m t trong nh ng i t ng nui th y s n ang c pht tri n Vi t Nam. L m t trong nh ng loi c c gi tr xu t kh u cao. c nhi u th tr ng a chu ng v mu s c c th t tr ng, th t c th m ngon h n so v i cc loi c catfish khc.

M c basa
Thnh ph n: Triglyceride: 85-90 % Diglyceride, monoglyceride Acid bo t do RCOOH Ch t mu, phospholipid v vitamin

2. N i dung v ph ng php nghin c u

2.1 N i dung nghin c u:
Bao g m hai n i dung chnh: T ng h p hai lo i CH BM sinh h c anion t m c basa quy m phng th nghi m. nh gi ch t l ng s n ph m thng qua kh n ng t o nh , ch s CMC, HLB.

Quy trnh t ng h p CH BM sinh h c anion t m c basa

2.2 Ph

ng php nghin c u

M c l h n h p ester c a glycerol v i cc acid bo no v khng no. Acid oleic chi m t l cao nh t l 33,6%. xc nh c u trc c a s n ph m CH BM sinh h c anion, v c ng nh tm i u ki n t i u cho ph n ng.

Ch n acid oleic lm nguyn li u

kh o st.

2.2 Ph

ng php nghin c u

Kh o st cc y u t nh h ng n hi u qu c a cc qu trnh chuy n ha: t l mol tc ch t, th i gian, nhi t , l ng ch t xc tc. Trong qu trnh kh o st, y u t kh o st t t nh t l y u t m khi ph n ng k t thc th tc ch t chnh ph n ng h t thng qua vi c ki m tra b ng s c k l p m ng. Qu trnh kh o st c ti n hnh trn c s thay i m t y u t c n kh o st v c nh cc y u t cn l i. Qu trnh c l p l i v i cc y u t cn l i trn c s s d ng k t qu t i u c a cc y u t v a tm c. Xc nh c u trc c a s n ph m b ng ph ng php o ph 1H-NMR v 13C-NMR, ph DEPT.

3. K t qu v th o lu n
3.1 Kh o st trn ngu n nguyn li u acid oleic 3.1.1 Ph n ng ester ha gi a acid oleic v acid lactic
COOH Acid oleic (1) CH3 HO COOH p-TsOH, to O Acid 2-(oleoyloxy)propanoic (3) O COOH CH3

-CH2-CH2-CH= -CH3 -CH2-CO-CH=CH-COOH

-CH2-CH= -CH2-CH2-CO-

-CH=CH-COOH

-CH3

-CH=CH-

-CH=CH-

-CH3

-CH2-CH= -CH2-CO-

-CH2-

-CH=CH-COOH

-CH2-CO- -CH2-CH3 -CH2-CH=

-CH-COOH -CH=CH-COOH

-CH2-CH= -CH-CH3

-CH2-CH3

-CH2-CO-

-CH2-CH2-CH= -CH-CH3 -CH2-CO-CH3 -CH=CH-COO-CH-COOH

-CH=CH-

-CH-COOH

-CH=CH-

-CH-COOH

-CH-CH3

-CH3

-CH2-CH= -CH2-CH2-CO-CH=CH-COO-CH-COOH -CH3

Kh o st cc y u t nh h ng n hi u su t c a ph n ng ester ha gi a acid oleic v acid lactic:

1. T l mol tc ch t 2. Th i gian ph n ng 3. Nhi t 4. L ph n ng ng ch t xc tc

Kh o st nh h

ng c a t l mol tc ch t

Cc y u t c Nhi t ph n ng Th i gian ph n ng Xc tc p-TsOH (% so v i l T c khu y (vng/pht)

nh

Gi tr 150C 8 gi

ng acid oleic)

5% 700

Y u t thay i l t l mol tc ch t acid oleic v acid lactic, t ng ng l: 1:7, 1:8, 1:9, 1:10

B n m ng kh o st nh h ng c a t l mol tc ch t (EtOAc:MeOH=5:2)

1:7

1:8

1:9

1:10

T l mol t t nh t

Kh o st nh h

ng c a th i gian ph n ng

Cc y u t c nh T l mol tc ch t Nhi t ph n ng Xc tc p-TsOH (% so v i l ng acid oleic) T c khu y (vng/pht) Y u t thay i l th i gian ph n ng, l n l kho ng: 6 gi , 7 gi v 8 gi .

Gi tr 1:10 150C 5% 700 t cc

B n m ng kh o st nh h ng c a th i gian ph n ng (EtOAc:MeOH=5:2)

Th i gian t t nh t
6 gi 7 gi 8 gi

Kh o st nh h Cc y u t c T l mol tc ch t Th i gian ph n ng Xc tc p-TsOH (% so v i l T c khu y (vng/pht) i l: nhi t

ng c a nhi t nh

ph n ng Gi tr 1:10 8 gi 5% 700

ng acid oleic)

Y u t thay

130C, 140C v 150C

B n m ng kh o st nh h ng c a nhi t ph n ng (EtOAc:MeOH=5:2)

Nhi t
130C 140C 150C

t t nh t

Kh o st nh h xc tc
Cc y u t c T l mol tc ch t Th i gian ph n ng Nhi t T c ph n ng khu y (vng/pht) nh Gi tr 1:10 8 gi 150C 700

ng c a l

ng ch t

4% 5% EtOAc:MeOH=5:2 Y

u t thay

i: l

ng ch t xc tc 4% v 5%

 K t lu n: l

ng ch t xc tc thch h p nh t l 5%

T ng k t i u ki n t i u c a ph n ng ester ha gi a acid oleic v acid lactic

T l mol tc ch t = 1:10 Th i gian ph n ng: 8 gi Nhi t ph n ng: 150C L ng ch t xc tc: 5% so v i kh i l ng acid oleic T c khu y: 700 vng/pht
EtOAc:MeOH=5:2

Hi u su t: 84,42%.

3.1.2 Ph n ng transester ha gi a methyl oleate v acid lactic

T ng h p methyl oleate
Cc y u t T l mol acid oleic : methanol Xc tc H2SO4 c Gi tr 1:10 5% k/l acid oleic 4 gi 80C 600 vng/pht

Th i gian ph n ng Nhi t T c ph n ng khu y

T ng h p methyl oleate

Methyl oleate

Hi u su t ph n ng 88,9%

PE:EtOAc=3:1

-COOCH3 -CH2-CH=

-CH2-

-CH3 -CH2-CO-CH=CH-

Ph n ng transester ha gi a methyl oleate v acid lactic

Kh o st cc y u t nh h ng n hi u su t c a ph n ng transester ha gi a methyl oleate v acid lactic:

1. T l mol tc ch t 2. Th i gian ph n ng 3. Nhi t 4. L ph n ng ng ch t xc tc

Kh o st nh h

ng c a t l mol tc ch t

Cc y u t c Nhi t ph n ng Th i gian ph n ng Xc tc p-TsOH (% so v i l T c khu y (vng/pht)

nh

Gi tr 160C 10 gi

ng acid oleic)

5% 700

Y u t thay acid lactic, t

i l t l mol tc ch t acid oleic v ng ng l: 1:5, 1:6, 1:7

B n m ng kh o st nh h ng c a t l mol tc ch t (EtOAc:MeOH=5:2)

1:5

1:6

1:7

T l mol t t nh t

Kh o st nh h

ng c a th i gian ph n ng

Cc y u t c nh T l mol tc ch t Nhi t ph n ng Xc tc p-TsOH (% so v i l ng acid oleic) T c khu y (vng/pht)

Gi tr 1:7 160C 5% 700 t

Y u t thay i l th i gian ph n ng, l n l cc kho ng: 8 gi , 9 gi v 10 gi .

B n m ng kh o st nh h ng c a th i gian ph n ng (EtOAc:MeOH=5:2)

8 gi
Th i gian t t nh t

9 gi

10 gi

Kh o st nh h Cc y u t c T l mol tc ch t Th i gian ph n ng Xc tc p-TsOH (% so v i l T c khu y (vng/pht) i l: nhi t

ng c a nhi t nh

ph n ng Gi tr 1:7 10 gi 5% 700

ng acid oleic)

Y u t thay

140C, 150C v 160C

B n m ng kh o st nh h ng c a nhi t ph n ng (EtOAc:MeOH=5:2)

140C

150C

160C

Nhi t

t t nh t

Kh o st l

ng ch t xc tc
Gi tr 1:7 10 gi 160C 700

Cc y u t c nh T l mol tc ch t Th i gian ph n ng Nhi t ph n ng T c khu y (vng/pht)

Y

u t thay

i: l

ng ch t xc tc 2%, 3%, 4% v 5%

B n m ng l ng ch t xc tc (EtOAc:MeOH=5:2)

2%

3%

4%

5%

 K t lu n: l

ng ch t xc tc thch h p nh t l 5%

T ng k t i u ki n t i u c a ph n ng transester ha gi a methyl oleate v acid lactic

T l mol tc ch t = 1:7 Th i gian ph n ng: 10 gi Nhi t L T c ph n ng: 160C khu y: 700 vng/pht ng ch t xc tc: 5% so v i k/l acid oleic

Hi u su t: 77,18%.

So snh hai ph ng php t ng h p ester (3) t nguyn li u acid oleic

Ph ng php ester ha (hi u su t 84,42%) v transester ha (hi u su t 77,18%). Hi u su t ph n ng chnh l ch khng nhi u nh ng ph ng php transester ha c nhi u u i m h n: + Gi m c t l mol: ph ng php transester ha c t l mol l 1:7; trong khi ph ng php ester ha c t l mol l 1:10. + Khi p d ng ln m c basa, cch t ng h p t methyl ester s cho hi u qu kinh t . K t lu n: ch n ph ng php transester ha cho cc b c t ng h p ti p theo.

So snh hai ph ng php t ng h p ester (3) t nguyn li u acid oleic

Ph ng php ester Ph ng php transester

T l mol acid oleic v acid lactic: 1:10 Nhi t : 150C Th i gian: 8 gi Xc tc p-TsOH: 5% Hi u su t: 84,42%

T l mol methyl oleate v acid lactic: 1:7 Nhi t : 160C Th i gian: 10 gi Xc tc p-TsOH: 5% Hi u su t: 77,18%

Khi t ng h p t nguyn li u m c
M c X phng ha Acid ha Acid bo t do

M c

CH3OH/KOH

Methyl ester

T ng h p methyl ester s cho hi u qu kinh t . K t lu n: ch n ph ng php transester ha cho cc b c t ng h p ti p theo.

Giai o n base ha
NaOH

Acid 2-(oleoyloxy)propanoic

Sodium 2-(oleoyloxy)propanoate (Hi u su t 58,36%)

Hydroxy ha methyl oleate

Cc y u t T l mol methyl oleate : H2O2 : HCOOH Th i gian ph n ng Nhi t T c ph n ng khu y Gi tr 1:2:4 2 gi 65C 600 v/ph

PE:EtOAc=3:1

Transester ha h n h p
Cc y u t T l mol s n ph m : MeOH Xc tc KOH Th i gian ph n ng Nhi t T c ph n ng khu y Gi tr 1:5 1% k/l s n ph m 2 gi 65C 600 vng/pht

PE:EtOAc=3:1 Hi u su t 86,7%

Methyl 9(10)-hydroxy-10(9)-formyloxystearate -COOCH3 -CH-OH -CH-OCOH -OCOH -CH2-CO-CH2-CH-

-CH2-

-CH3

-COOCH3 -CH2-CH-CH2-CO-OH -CH-OH

-CH2-

-CH3

-CH2-CH-CH2-CO-CH-OH -CH2-

-OCH3 -COO-

-CH3

-CH-OH

-CH-OH

-OCH3

-CH3

-CH2-CO-

-CH2-CH2-CH-

-CH-OH -OCH3 -COO-

-CH3

3.1.3 Ph n ng transester ha gi a methyl 9,10-dihydroxystearate (5) v acid lactic

-CH2-CH2-CH -CH-OCO-CH-COOH HO-CH-CH3 -CH-CH3 -CH2-CO-CH-OH -CH3

-CH2-CH-CH-CH3 HO-CH-CH3 -CH-OCO-CH-COOH -CH-OH -COO-CH2-CO-CH3 -CH2-

-CH-COOH

-CH-OCO-

HO-CH-CH3

-CH-OH

-CH-CH3 -CH-CH3

-CH3

-CH2-CO-CH2-CH- -CH2-

-COO-

Kh o st cc y u t nh h ng n hi u su t c a ph n ng transester ha gi a tc ch t (5) v acid lactic:

1. T l mol tc ch t 2. Th i gian ph n ng 3. Nhi t 4. L ph n ng ng ch t xc tc

Kh o st nh h

ng c a t l mol tc ch t

Cc y u t c Nhi t ph n ng Th i gian ph n ng Xc tc p-TsOH (% so v i l T c khu y (vng/pht)

nh

Gi tr 160C 8 gi

ng acid oleic)

5% 700

Y u t thay i l t l mol tc ch t (5) v acid lactic, t ng ng l: 1:7, 1:8, 1:9

B n m ng kh o st nh h ng c a t l mol tc ch t (EtOAc:MeOH=5:1)

1:7

1:8

1:9

T l mol t t nh t

Kh o st nh h

ng c a th i gian ph n ng

Cc y u t c nh T l mol tc ch t Nhi t ph n ng Xc tc p-TsOH (% so v i l ng acid oleic) T c khu y (vng/pht) Y u t thay i l th i gian ph n ng, l n l kho ng: 6 gi , 7 gi v 8 gi .

Gi tr 1:9 160C 5% 700 t cc

B n m ng kh o st nh h ng c a th i gian ph n ng (EtOAc:MeOH=5:1)

6 gi

7 gi

8 gi

Th i gian t t nh t

Kh o st nh h Cc y u t c T l mol tc ch t Th i gian ph n ng Xc tc p-TsOH (% so v i l T c khu y (vng/pht) i l: nhi t

ng c a nhi t nh

ph n ng Gi tr 1:9 8 gi 5% 700

ng acid oleic)

Y u t thay

140C, 150C v 160C

B n m ng kh o st nh h ng c a nhi t ph n ng (EtOAc:MeOH=5:1)

140C 150C 160C

Nhi t

t t nh t

Kh o st l

ng ch t xc tc
Gi tr 1:9 8 gi 160C 700

Cc y u t c nh T l mol tc ch t Th i gian ph n ng Nhi t ph n ng T c khu y (vng/pht)

Y

u t thay

i: l

ng ch t xc tc 3%, 4% v 5%

B n m ng l ng ch t xc tc (EtOAc:MeOH=5:2)

3%

4%

5%

 K t lu n: l

ng ch t xc tc thch h p nh t l 5%

T ng k t i u ki n t i u c a ph n ng transester ha gi a tc ch t (5) v acid lactic

T l mol tc ch t = 1:9 Th i gian ph n ng: 8 gi Nhi t L T c ph n ng: 160C ng (5) khu y: 700 vng/pht ng ch t xc tc: 5% so v i kh i l

Hi u su t: 72,64%.

Giai o n base ha

NaOH

9(10)-hydroxy-10(9)-lactyloxystearoyl lactic acid

Sodium 9(10)-hydroxy-10(9)-lactyloxystearoyl lactylate (Hi u su t 56,29%)

3.2 T ng h p CH BM sinh h c anion t m c basa

T ng h p methyl ester
Cc y u t M c basa (g) Methanol (g) KOH (g) Th i gian ph n ng Nhi t ph n ng T c khu y Gi tr 84 16 1 2 gi 65C 600 vng/pht

T ng h p methyl ester

PE:EtOAc=14:1

Mehtyl ester (Hi u su t 89,9%)

Dihydroxy ha methyl ester

Cc y u t Th i gian ph n ng Nhi t T c ph n ng khu y Gi tr 2 gi 65C 600 v/p

T l mol methyl ester : H2O2 : HCOOH 1 : 1,5 : 3

Dihydroxy ha methyl ester

PE:EtOAc=3:1

Transester ha h n h p
Cc y u t T l mol h n h p : methanol Xc tc KOH Th i gian ph n ng Nhi t T c ph n ng khu y Gi tr 1:5 1% k/l h n h p 2 gi 65C 600 v/ph

Transester ha h n h p

PE:EtOAc=3:1

S n ph m dihydroxy ha methyl ester (Hi u su t 62,9%).

T ng h p sodium acyl lactylate (4')

CH3 O R C OCH3 HO COOH p-TsOH, to CH3 O O NaOH R C R C O CH COOH Acyl lactic acid (3') CH3 O CH COONa Sodium acyl lactylate (4')

Methyl ester (2')

Cc y u t T l mol methyl ester : acid lactic Th i gian ph n ng Nhi t ph n ng T c khu y Xc tc p-TsOH

Gi tr 1:7 10 gi 160C 700 vng/pht 5% so v i kl methyl ester

T ng h p sodium acyl lactylate (4')

S n ph m CH BM anion (4')
EtOAc:MeOH=5:2

(Hi u su t 61,32%)

Hi u su t 75,18%

T ng h p sodium acyl lactylate CH (7')

3

O R C OCH3 Methyl ester (2')

HCOOH/H2O2

O R' C OCH3 (5')

HO COOH p-TsOH, to

O R'' C

CH3 O CH COONa (7')

NaOH

CH3 O R'' C O CH COOH (6') O

R= R'= HO OH R''=

O OH

OH

T ng h p sodium acyl lactylate (7')

Cc y u t c Nhi t nh Gi tr 1:9 160C 8 gi 700 vng/pht 5% so v i kl (5') T l mol tc ch t (5') : acid lactic ph n ng Th i gian ph n ng T c khu y

Xc tc p-TsOH

T ng h p sodium acyl lactylate (7')

EtOAc:MeOH=5:1

S n ph m CH BM anion (7') (Hi u su t 57,46%)

Hi u su t 68,53%

3.3 nh gi ch t l

ng s n ph m

CH BM sinh h c anion t ng h p t m c basa c nh gi ch t l ng s b qua cc ch tiu nh :

+ Kh n ng t o nh + Gi tr CMC + Ch s HLB

nh gi kh n ng t o nh
Pha tr n cc t l khc nhau v % kh i l CH BM anion, paraffin v n c. Cc y u t c Nhi t T c khu y khu y Th i gian khu y nh Gi tr 30C 15 pht 600 vng/pht ng c a:

nh gi kh n ng t o nh c a CH BM (4')
T l % v kh i l ng trong h n h p 30% CH BM + 35% n c + 35% paraffin 30% CH BM + 30% n c + 40% paraffin 30% CH BM + 25% n c + 45% paraffin 30% CH BM + 15% n c + 55% paraffin 30% CH BM + 10% n c + 60% paraffin 25% CH BM + 40% n c + 35% paraffin 25% CH BM + 35% n c + 40% paraffin 20% CH BM + 50% n c + 30% paraffin 20% CH BM + 30% n c + 50% paraffin K t qu khng b n khng b n khng b n khng b n Nh b n khng b n khng b n khng b n khng b n

Nh Nh Nh Nh Nh Nh Nh Nh

nh gi kh n ng t o nh c a CH BM (7')
T l % v kh i l ng trong h n h p 30% CH BM + 45% n c + 25% paraffin 30% CH BM + 40% n c + 30% paraffin 30% CH BM + 35% n c + 35% paraffin 30% CH BM + 30% n c + 40% paraffin 30% CH BM + 20% n c + 50% paraffin 25% CH BM + 35% n c + 40% paraffin 25% CH BM + 30% n c + 45% paraffin 20% CH BM + 25% n c + 55% paraffin 20% CH BM + 30% n c + 50% paraffin Nh Nh Nh Nh Nh Nh Nh Nh K t qu khng b n khng b n khng b n khng b n Nh b n khng b n khng b n khng b n khng b n

Ch p hnh h nh t ng b n d i knh hi n vi i n t

CH BM anion (4')

CH BM anion (7')

Xc

nh n ng

micelle t i h n (CMC)

Trong dung d ch n c: khi v t m t n ng no th cc phn t CH BM s t t p h p l i t o thnh micelle. N ng micelle t i h n l n ng m t i cc phn t CH BM b t u t o micelle. Chnh s hnh thnh cc micelle lm thay i tnh ch t v t l (s c c ng b m t, d n i n) c a cc dung d ch CH BM.

 Xy d ng th bi u di n s thay i c a i n theo n ng c a dung d ch CH BM. Gi tr CMC S thay i v do cc micelle c xc nh t th ny.

d n

d n i n ring m t cch t ng t l c hnh thnh m t cch b t ng .

V v y, m t n ng no c m t s gin o n x y ra c a d n i n c a dung d ch c g i l n ng micelle t i h n.

Xc
N ng

nh gi tr CMC c a CH BM (4')
0 5 6 7 8 9 10

CH BM anion (g/l)
mS/cm

d n i n (mS/cm)
3 2.5 2 1.5

0,33 0,56 0,75 0,95 1,17 1,83 2,47

2.47

CMC
1.83

1.17 1 0.75 0.5 0 5 6 7 8 9 10 11 g/l 0.56 0.95

Xc
N ng

nh gi tr CMC c a CH BM (7')
0 2 4 6 8 10 12

CH BM anion (g/l)

d n i n (mS/cm)
12 10 mS/cm

0,33 2,16 4,14 5,02 7,28 9,51 10,75

10.75

CMC
8 7.28 6 5.02 4 2 0 0 0.33 2 4 6 8 10 2.16 4.14

9.51

g/l 12 14

Xc nh h ng s cn b ng a n n c (HLB)
L s cn b ng t ng ng gi a ph n a n ph n k n c c a CH BM.

c-k

c v

Bi u th m i quan h v i n c v v i d u c ng nh khuynh h ng nh ha c a CH BM.

M i lin h gi a tnh ch t c a CH BM v i gi tr HLB c trnh by trong b ng sau: Tnh ch t Khng phn tn trong n Nh W/O Ch t th m t Nh O/W Ch t t y r a Ch t h tr ha tan c HLB 1-3 3-6 7-9 8-15 13-15 15-18

Xc

nh gi tr HLB c a CH BM (4)
O O Sodium 2-(oleoyloxy)propanoate Sodium oleoyl lactylate (4) COONa CH3

Nhm a n c g m: nhm -COONa c k/l mol l 67 g, nhm carboxyl -COO c k/l mol l 44 g. K/l mol ton phn t (4): 376 g V y HLB c a (4) l: 20.[(67+44)/376]=5,9 V HLB=5,9 nn (4) ha tan trong n d u nn c kh n ng t o nh W/O. c t h n trong

Xc

nh gi tr HLB c a CH BM (7)
OH O HO H3 C O O O COONa CH3

Sodium 9(10)-hydroxy-10(9)-lactyloxystearoyl lactylate (7)

Nhm a n c g m: nhm -COONa c k/l mol l 67 g; 2 nhm carboxyl -COO c k/l l 44.2=88 g; 2 nhm hydroxyl OH c k/l l 17.2=34 g. Kh i l ng mol ton phn t (7): 482 g ng d ng lm ch t th m t. V y HLB c a (7) l: 20.[(67+88+34)/482]=7,8 V HLB=7,8 nn c th

 Gi tr HLB tnh ton c theo cng th c ton h c khng c p n m t c tnh quan tr ng trong phn t ch t nh ha, l c u trc khng gian c a phn t . C u trc khng gian c a phn t CH BM c nh h ng r t l n n kh n ng t o nh t ng v v ng b n c a nh t ng t o thnh. V v y, v ng b n c a m t h nh t ng c i u ch nh ch t nh ha c thnh ph n ha h c nh nhau, gi tr HLB c tnh ton nh nhau nh ng c c u trc khng gian khc nhau th v ng b n c a nh t ng thu c r t khc nhau.

4. K t lu n v ki n ngh 4.1 K t lu n
+ Tm c i u ki n t i u t ng h p hai CH BM sinh h c anion (4') v (7') t m c basa quy m phng th nghi m. + nh gi s n ph m qua kh n ng t o nh , ch s CMC, HLB.

4.2 Ki n ngh
Xc nh thm kh n ng t o b t, kh n ng t o huy n ph, kh n ng th m t v kh n ng kch ng da c a CH BM t ng h p c. Thay i cation Na+ trong c u trc c a s n ph m CH BM thnh cc cation khc nh : Ca2+, K+, NH4+,... Nghin c u kh n ng ng d ng c a s n ph m CH BM t ng h p c trong cc l nh v c s n xu t: ch t t y r a, m ph m, d c ph m, cng ngh th c ph m,

Cm n s quan tm theo di c a qu th y c v cc anh ch !