Sn xut ch phm vi sinh vt

I.
Sn xut N:
1. Sn xut cp 1:
Mi trng cp 1( MTC1) l ng nghim thch nghing. ng nghim
(5ml/ng), kh trng vi p sut 0.8-1at/30 pht. Sau kh trng, ng
nghim nghing vt trn mt phng, ngui, ro mt thch th c th
cy vi khun vo c. ng cp 1 c nui trong t m t 28 -30 trong
48 gi.
- glucose: 10g
-saccharose: 10g
- CaCO3: 5g
- K2HPO4: 0.9g
- KH2PO4: 0.2g
- MgSO4.7H2O: 0.5g
- FeCl3.6H2O: 0.1g
- Na2MoO4.2H2O: 0.05g
- Agar: 18g.
- H2O: 1l
2. Sn xut cp 2:
Mi trng cp 2 l dch c phn v cc bnh tam gic 500ml : 300ml
dch mi trng/ bnh. Kh trng t 0.8-1at/30 pht. Khi dch nui cy
ngui, ly ng ging cp 1 cy vo bnh C2 theo t l 1:1. Dng que cy
v trng gt ly sinh khi. nh tan sinh khi trong dch ri sang bnh
dch nui cy cp 2, thao tc trong t v trng. C2 c nui lc
180vng/pht, 28-30 trong 48 gi. Ch : nu ngng lc s nh hng
n mt VSV.
MTC2 ging MTC1 ko c agar
3. Sn xut cp 3:
Dch MTC3 c lm vo bnh 1l: 600ml. Kh trng nh trn. ngui
ri cy 1 bnh C2 cho 3 bnh C3. Thao tc dch t C2 sang C3 c
thc hin trong t v trng. Ging C3 nu c k th nui lc, khng th
nui tnh, nhit t 28-30 trong 48 gi. MTC3 ging MTC2 ch khc l
dng CaCO3 cng nghip.
II.
Sn xut P
V nguyn tc ging sx N nhng Mt dch nui cy ko cn lc m tnh
- glucose (saccharose): 10g
- (NH4)2SO4: 0.5g
- NaCl: 0.2g
- Ca3(PO4)2: 5g
- KCl : 0.2g

- MnSO4: 0.01g
-FeSO4.7H2O : 0.01g

-MgSO4.7H2O: 0.1g
- YE: 6.5g
- Agar: 18g
- H2O: 1l
MTC2,3
- glucose (saccharose): 10g
- (NH4)2SO4: 0.5g
- NaCl: 0.2g
- Ca3(PO4)2: 5g
- NaCl : 0.2g
- dung dch vi lng: 1ml
-H2O: 1l
(Note: dung dch vi lng:
- H3BO3: 5g;
- (NH4)2MoO4: 5g;
- ZnSO4: 0.2g;
- AlCl3: 0.15g;
-H2O: 1l
III. Sn xut ch phm
Cht mang ch phm dng than bn. Than bn c hong kh v trn
vi 1% CaCO3, ng ti polymer, kh trng 1-1.5at/1gi. Than bn kh
trng ngui ri cy dch C3 theo t l 3% ( C3N:C3P l 1:1, ho u
dch trc khi cy). Ch phm sn xut trong mi trng sch tit
trng, sn xut trong ngy, khng dch nui cy sang ngy hm sau.
Than bn trn dch nui cy c m 40%, ng ti 50g/ ti.
IV. Kim tra cht lng ch phm
Kim tra mt vi sinh sau 24 gi, 1 thng, 2 thng, 3 thng sau sn
xut. Mi trng kim tra dng MTC1 vi N v MTC2 vi P. Pha long
ch phm v cy gt trn b mt thch. Nui trong t 28-30 trong 48 gi.
m s lng t bo t 108 -109 l c. VSV ng nht, ko tp nhim.