3.1.1.1.

Hm lng ng tng s ( theo phng php Ixekutz)

Nguyn tc: tin hnh thy phn acid chuyn dng ng kh thnh ng
khng kh, sau tin hnh xc nh ng kh theo phng php chun iod.
Tin hnh:
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Chit rt ng: Cn 1g mu sy kh cho vo ci s, thm mt vi git nc ct,

nghin k thnh dng ng th. Cho 20ml nc ct vo ci, nghin tip, lng, cht ly
phn nc vo cc. Sau lp li qu trnh trn 3 ln, dng nc ct trng li ci, chy s.
Chuyn ton b dch mu sang bnh nh mc (100ml), dng nc ct a th tch n
vch. Lc dch chit qua bng, c dch trit trong xc nh ng kh.
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Chuyn dng ng khng kh thnh ng kh: Ly 5ml trong 100ml dch chit

ng thm vo 2ml HCL 6N. t trn bp cch thy nhit 70 80oC trong thi gian
20 pht. Thnh thong lc u, cc ng kp c thy phn thnh ng n. Sau
khi ngui cho vo bnh 3 git phenolphtalein 1% trong cn 96o. Trung ha lng acid
cn d bng dung dch NaOH 10% cho n khi c mu hng. Lm chua li bng dung dch
CH3COOH 10% cho n khi mt mu. Dng nc ct ln th tch n vch, c dung
dch ng kh.
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nh lng ng kh bng phng php Ixekutz

o

Nguyn tc: trong mi trng kim, ng kh Fe3+ trong Kali Ferxianua

thnh Fe2+ .
K3Fe(CN)6 + ng kh

K4Fe(CN)6 + Sn phm oxi ha ca ng

cho phn ng xy ra hon ton, dng ZnSO4 to kt ta vi K4Fe(CN)6

2K4Fe(CN)6 + ZnSO4
Lng K3Fe(CN)6 + 2KI

K2Zn3[Fe(CN)6]2 + 3K2SO4
K4Fe(CN)6 + I2

I2 gii phng ra c chun bng Na2S2O3

Na2S2O3 + I2

Na2S4O6 + 2NaI

T lng Na2S2O3 tiu tn, ta tnh c lng ng kh c trong dung

dch.
o Tin hnh: ly 2 bnh tam gic, bnh 1 (i chng), bnh 2 ( th nghim).
Cho vo bnh 1: 5ml nc ct, bnh 2: 5ml dch chit ng
Cho vo mi bnh 10ml K3Fe(CN)6 0.05N. un si 1 pht trn bp in,
ngui . Cho vo mi bnh 10ml hn hp (KI + ZnSO4), 10ml CH3COOH 10%.

Chun bng Na2S2O3 0.05N cho n khi c mu vng rm. Thm 3 git
tinh bt (dung dch chuyn mu xanh), chun tip cho n khi c mu trng sa
hon ton.
Ch : nu sau khi cho (KI + ZnSO4) m dung dch c mu trng sa
ngay, c ngha l lng ng trong dung dch qu nhiu, cn phi lm li vi
lng mu t hn.
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nh lng ng tng s

X: hm lng ng tng s (%)

a: lng ng glucose trong dung dch xc nh (mg)
v: th tch dung dch mu ngu nhin nghin cu (5ml)
V: ton b th tch dch mu chit (100ml)
C: Lng mu phn tch (1g)
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Xc nh lng ng glucose trong dung dch xc nh a (mg)

o Bnh i chng chun ht 9.5ml Na2S2O3 0.05N ta c t s:

Chun bnh th nghim ht mt lng Na2S2O3 0.05N, th lng

K3Fe(CN)6 d c xc nh nh sau:
y: lng K3Fe(CN)6 d (ml)
x: lng Na2S2O3 0.05N chun bnh th nghim (ml)
Gi z l lng K3Fe(CN)6 0.05N phn ng vi ng kh: z=10-y(ml)
T lng K3Fe(CN)6 0.05N phn ng vi ng, tnh lng ng kh
(glucose) c trong dung dch nghin cu theo bng 3.1.
Bng 3.1. Tnh hm lng ng glucose theo phng php Ixekutz

0ml
0.00

0.00
-

0.10
1.15

0.20
-

0.30
-

1.00

1.50

1.67

1.83

2.00

2.00
3.00
4.00
5.00
6.00

3.10
4.72
6.37
8.06
9.72

3.26
4.88
6.54
8.22
9.89

3.24
5.64
6.71
8.39
10.06

3.58
5.20
6.88
8.56
10.23

0.40
2016.
00
3.74
5.36
7.05
8.72
10.41

0.50
0.73

0.60
0.87

0.70
1.02

0.80
1.18

0.90
1.34

2.13

2.47

2.62

2.78

2.94

3.90
5.53
7.22
8.89
10.58

4.06
5.70
7.39
9.06
10.75

4.22
5.86
7.55
9.22
10.92

4.38
6.03
7.72
9.39
11.10

4.54
6.20
7.80
9.55
11.28

7.00
8.00
9.00

11.46
13.28
14.99

11.64
13.46
-

11.82
13.63
-

12.00
13.80
-

12.18
13.97
-

12.36
14.14
-

12.54
14.31
-

12.73
14.49
-

12.91
14.66
-

13.10
14.83
-