TRNG I HC CNG NGHIP TP.

H CH MINH KHOA CNG NGH HA HC TIU LUN MN : PHN TCH CNG NGHIP

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TI : XC NH NG THI Ca, Mg, Al, Fe, Mn, Mg, Zn TRONG PHN BN V C

GVHD : TRN NGUYN AN SA SVTH : NGUYN C NAM MSSV : 09077331

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NI DUNG

I CNG

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PHN BN V C
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Phn v c l cc loi mui khong c cha cc cht dinh dng ca cy. Thnh phn : Nguyn t a lng : N, P, K Nguyn t trung lng : Ca, Mg, S Nguyn t vi lng: Fe, Mn, Zn, Cu, Mo, B, Cl. Ngoi ra, cn mt s nguyn t khc cn thit cho tng loi cy nh: Na, Si, Co, Al

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PHNG PHP PH NGUYN T

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Phng php o ph hp th nguyn t l mt k thut phn tch ha l v ang c ng dng rng ri trong nhiu ngnh khoa hc k thut, trong sn xut cng nghip, nng nghip, y dc, a cht, ha hc. Nguyn tc v l thuyt : Tt c cc nguyn t u c kh nng hp th nh sng Mi mt nguyn t hp th nh sng vi mt bc sng c trng Phng php ny l mt trong nhng cng c c lc xc nh hm lng cc kim loi nng v nhng nguyn t c hi trong t nhin .

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Xc nh ng thi cc nguyn t bng phng php ph nguyn t AAS

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PHM VI NG DNG
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Theo tiu chun AOAC 965.09 Theo tiu chun ngnh 10TCN 362-99 p dng xc nh ng thi lng vt Ca, Cu, Fe, Mn, Mg,Al v Zn c trong phn bn v c c gii hn nng t ti ppm.

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Thit b
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My hp th nguyn t AAS v cc n catot rng Cu, Zn, Mo, Co Cc bnh nh mc 1000, 50, 25 (ml) Cc pipet 10, 5, 2, 1 (ml) Cn phn tch c chnh xc 0.0002 g Bnh cng ph Bp cng ph Phu lc c ng knh 8 cm Bnh nh mc 50 ml

Thuc th
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HCL (PA) HNO3 Dung dch HCL 6N Hn hp 2 axit HNO3 c v HCl t l 1: 3(s dng ngay) Nc ct 2 ln hoc nc lc siu sch c dn in < 0.01s/cm Cc dung dch tiu chun 1000 ppm ( Cu, Zn, Mn, Fe,Al,Mg,Mn)

Quy trnh phn tch

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Nguyn tc
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Phn hy v ha tan cc nguyn t vi lng trong cc mu phn bng hn hp HNO3 v HCl m c, xc nh hm lng cc nguyn t bng my hp thu nguyn t AAS

Cch tin hnh

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Quy Trnh
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p dng AAS theo ch dn m hng ch to cung cp. Thnh lp dy chun ca cc nguyn t Cu, Mn, Fe, Zn, Al, Fe, Mg, Ca trong dung dch HCl 0.5 M, nng ph hp vi khong o ca AAS. Pha long dung dch cn xc nh bng dung dch HCl 0.5 M cho ph hp vi khong xc nh. t dy tiu chun, mu trng v cc dung dch trn AAS

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PHA CH CC DUNG DCH CHUN

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Cc dung dch gc Ca,Cu, Fe, Mg, Mn, Zn, Al nh lng theo phng php ng chun, t dung dch gc tin hnh pha dung dch chun lm vic Dung dch gc Ca (25g Ca/mL) : 1.249g CaCO3, thm lng ti thiu HCl 3M pha long n 1 lt. Dung dch gc Cu (1000g Cu/mL) : 1g kim loi Cu nguyn cht vi mt lng ti thiu HNO3, thm 5mL HCl lm bay hi pha long n 1 lt vi HCl 0.1M

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PHA CH CC DUNG DCH CHUN

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Dung dch gc Fe (1000g Fe/mL) : 1g dy Fe nguyn cht, thm 30mL HCl 6M un nng pha long n 1 lt . Dung dch gc La (50g La/L) : 58.65g La2O3, thm 250 mL HCl(thm t t acid) pha long n 1 lt. Dung dch gc Mg (1000g Mg/mL) : 1g kim loi Mg nguyn cht, thm 50mL H2O, thm 10mL HCl (thm t t) pha long n 1lt.

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PHA CH CC DUNG DCH CHUN

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Dung dch gc Mn (1000g Mn/mL) : 1.582g MnO2, thm 30mL HCl 6M un ui Cl- pha long n 1 lt. Dung dch gc Zn (1000g Zn/mL) : 1g kim loi Zn nguyn cht, thm 10mL HCl 6M pha long n 1 lt. Dung dch gc Al (500g Al/mL) : ho tan chnh xc 0.5 g kim loi Al (hoc mnh dy c kh Al2O3 bm vo) vo 15 ml HCl 6M trong bnh nh mc 1 lt pha long nc ct n vch, lc u.

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CHUN B DUNG DCH PHN TCH

2-3 g MU PHN BNH CNG PH HA TAN C CN MU Nghin Nh Qua Ry 0.2 mm Hn Hp 2 axit c HNO3 v HCl un nh 5 ml HCl un Si 5 HA TAN pht LC NH MC

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THC HIN PHP O

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Ci t thng s o cho my:

Nguyn t Ca Cu Fe Mg Mn Zn Al Bc sng (nm) 422.7 422.7 324.7 248.3 285.2 279.5 213.8 309.3 Ngn la Air- C2H2 N2O- C2H2 Air- C2H2 Air- C2H2 Air- C2H2 Air- C2H2 Air- C2H2 N2O-C2H2 Phm vi (g/mL) 2 20 2 20 2 20 2 20 0.2 2 2 20 0.5 5 0-25 Thm La C th thm La Ch Thm La 1%, HCl 1% Dng n kh c bit

Ly 1 phn th tch mu em i o, ghi li kt qu v tnh ton.

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Cch tnh kt qu

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NHN XT
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S dng phng php AAS trong vic xc nh cc nguyn t trung lng v vi lng cng nh cc nguyn t khc trong phn bn cho mt kt qu vi n nh v sai s nh, chnh xc v chn lc cao, cng lc c th xc nh ng thi cc nguyn t khc nhau trong mu. Tuy nhin,h thng phn tch ca my kh tn km v phc tp cng nh xc nh c thnh phn nguyn t trong mu nhng khng ch ra c trng thi lin kt ca nguyn t.

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TI LIU THAM KHO

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AOAC 965.09 Nutrients (Minor) in Fertilizers, AAS Method. The Perkin-Elmer Corporation, Analytical Methods for Atomic Absorption Spectroscopy, The Perkin-Elmer Corporation , 1996 , trang 147. 10TCN 362-99 - Phn tch phn bn: Phng php xc nh mt s vi lng

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Thank You !