khng bit c phng php no mi xc nh 2 ch tiu ny ko?

? mong cc anh ch tin bi nu c tcvn hay ti liu lin quan th gip em lun c khng !! Cho bn, Bn cn phng php mi l sao ah? Mnh ch bit xc nh hm lng Nitrit v Nitrat bng phng php o quang thi ah. diepgl . Phng php th tch Ngi ta c th xc nh nitrat theo phng php ny da trn phn ng kh NO3v cc trng thi oxi ho thp hn bng cc cht kh thch hp. Sau tin hnh php chun ( c th s dng chun trc tip hay chun ngc). Vi php chun ngc th mt lng chnh xc dung dch chun Fe2+ c cho d so vi lng cn thit vo dung dch mu. Sau lng d Fe2+ c chun bng dung dch Cr2O72+ vi cht ch th l ferroin. Cc phn ng xy ra nh sau: NO3- + 3Fe2+ + 4H+ ---> NO + 3Fe3+ + 2H2O 2Fe2+ + Cr2O72+ + 14H+ ---> 6Fe3+ + 2Cr3+ + 7H2O Phn ng gia Fe2+ v NO3- xy ra nhanh hn khi ung nng dung dch v c mt ca lng d axit H2SO4 65%. Do NO sinh ra phn ng vi oxi khng kh to thnh cc cht c kh nng b kh hay b oxi ho bi Fe2+ nn trong qu trnh phn ng v chun phi c tin hnh trong mi trng kh CO2. iu ny c thc hin bng cch thm mt lng nh NaHCO3 trc khi un nng v chun . 2. Phng php so mu Cc phng php so mu cng c dng xc nh NO3- da trn ba loi phn ng sau: + Nitrat ho cc hp cht phenolic. + Oxi ho cc hp cht hu c c nhm mang mu c trng. + Kh NO3- thnh NO2- hoc NH3 ri xc nh chng theo phng php thch hp. Trong phng php nitrat ho ch yu c s dng xc nh NO3- vi thuc th thng dng l axit phenol isunfonic Khi s dng thuc th axit phenol 2,4 isunfonic, ion NO3- phn ng vi axit ny to thnh axit nitro phenol isunfonic. Trong mi trng kim, axit nitro phenol isunfonic to thnh mt mui c mu vng cho hp th quang cc i bc sng 410nm. http://i321.photobucket.com/albums/nn378/tuxedomask_photo/Chemistry/2-2620098-50-31PM.png Phng php ny n gin v cho nhy kh cao (0,05ppm). Nhng khi c mt cc cht hu c, clorua, NO2-, cc ion c mu s gy nh hng n kt qu phn tch. Do cn phi loi b chng trc khi phn tch. S dng axit Sunfamin, ur, hay thiur tch loi NO2-. Cl- c loi b bng cch phn ng kt ta vi Ag2SO4. Loi tr nh hng ca cc hp cht hu c bng cch oxi ho bng

H2O2 hay s dng than hot tnh. Hin nay, chu u ngi ta ch nhiu n thuc th natri salixylat. Vi s c mt ca natri salixylat, nitrat to thnh hp cht c mu vng dng p - nitrosalixylat cho hp th quang cc i bc sng 410nm http://i321.photobucket.com/albums/nn378/tuxedomask_photo/Chemistry/2-2620098-50-52PM.png u im ca phng php ny l s dng n gin v c nhy cao. Tuy nhin, nu trong mu c mt cc hp cht hu c th lm thay i mu ca sn phm mu. Do , cn phi loi b cht hu c trc khi phn tch. Hn na, khi c mt Clorua s to thnh nitrosylclorua nh hng n kt qu phn tch. V vy, trc khi phn tch cng phi loi tr nh hng ca clorua 3. Phng php dng chy (FIA) FIA l mt phng php k thut phn tch ng, trong mu phn tch dng lng c bm trc tip vo dng cht mang chuyn ng lin tc. Sau mu i n vng phn ng, ri trong vng phn ng cht phn tch s phn ng vi thuc th c trong cht mang to ra sn phm c th pht hin c theo mt tnh cht ho l no ca n nh mt Dertector thch hp. Cc tnh cht ha l c th pht hin nh: + S hp th quang phn t UV-VIS. + S hp th quang nguyn t. + Tnh cht hunh quang. + S thay i chit sut trong pha ng. + S thay i in th. xc nh nitrat bng phng php FIA ngi ta tin hnh hai th nghim sau: http://www.flowinjection.com/method2_files/image002.jpg Th nghim 1: Xc nh hm lng NO2-. Trong mi trng axit yu ion NO2- phn ng vi thuc th sunfanyl amin v Netylen iamin mt cch nh lng v to thnh hn hp iazo hp th mnh bc sng 540 nm. Nu bm mu phn tch vo FIA c dng cht mang cha thuc th trn th c th xc nh c nng NO2- trong mu nh Dertector hp th quang UV-VIS bc sng 540 nm. Th nghim 2: xc nh tng NO3- v NO2-. Bng cch lp vo thm vo h FIA mt ct kh kh ion NO2- v NO3- sau xc nh tng hm lng NO2- nh th nghim mt. Hiu s kt qu ca c hai ln o chnh l hm lng nitrat. 4. Phng php cc ph Trong mi trng cht in li c in tch cao nh La3+ hay Ba2+, ion NO3- cho sng cc ph ti th t -1,1 n -1,4V.

 xc nh nitrat ngi ta thng dng sng xc tc uranin UO22+. Trong mi trng to phc nh nn Na2CO3 0,1M th UO22+ ch cho mt sng nh lng c E(1/2)=0,9-1,1V ph thuc nng NO3-. Kolthoff v cc cng s l nhng ngi u tin nghin cu xc nh NO3- bng dng cc ph xc tc. Cc tc gi cho rng trong nn HCl (0,1M) cha mt lng nh urani axetat s kh U(VI) xy ra theo hai bc: U(VI) + e ---> U(V) U(V) + e ---> U(III) to nn hai sng cc ph: Sng th nht ng vi s kh U(VI) xung U(V) c th bn sng E(1/2)=-0,18V Sng th hai ng vi s kh U(V) xung U(III) c th bn sng E(1/2)=-0,94V Khi c mt ca ion NO3- th chiu cao ca sng th hai tng ln t l tuyn tnh vi nng NO3- trong khong 5.10e-5- 4.10e-4 M. 5. Phng php o kh Hassan l ngi a ra mt phng php o kh n gin thch hp cho vic xc nh ng thi nitrat v nitrit trong cng mt mu. Theo ng, u tin nitrit c phn hu bng Ur ( CO(NH2)2 ) hoc axit sunfamit ( HSO3NH2 ) to ra kh N2 trong mi trng axit yu. Trong iu kin ny, nitrat khng tham gia phn ng. Kh Nit to ra c o bng mt trc m rt nh. Cc phn ng xy ra: 2KNO2 + CO(NH2) + 2HCl ---> 2N2 + CO2 + 3H2O + 2KCl KNO2 + HSO3 ---> N2 + KHSO4 + H2O Sau , un nng dung dch v to mi trng axit mnh th nitrat s phn ng to thnh kh N2O: 2KNO3 + CO(NH2)2 + 2HCl ---> 2N2O + CO2 + 3H2O + 2KCl 2KNO3 + HSO3NH2 + HCl ---> N2O + H2SO4 + H2O + KCl Kh N2 v N2O c gi li trong trc m k bng dung dch KOH trn 50%. 6. Phng php xc nh tng NO3- v NO2Ngi ta c th xc nh tng NO3- v NO2- trong cng mt mu bng phng php kh n gin l s dng hn hp Cd-Cu. Nguyn tc ca phng php nh sau: Ion NO3- b kh thnh ion NO2- vi s c mt ca Cd. Cc ht Cd c x l vi dung dch CuSO4, sau c np vo ct thu tinh. Phn ng kh tin hnh tt nht pH = 6-8. Hiu sut kh t 88-90%. Ion NO2- c xc nh nh phn ng to mu az ho bng axit sunfanilic v naphtylamin. Phc to thnh c cng mu ln. Cc i hp th bc sng 520nm. Phng php ny c dng phn tch NO3- vi nng nh hn 1ppm m cc

phng php khc khng nhy pht hin Bi vit copy trn mng ln s si nu pa cn qui trnh chun th mnh s gi cho, Rt mong c giao lu vi cc pro lm phng KCS YM dechepro mail diepaa1@gmail.com nguyencyberchem 10-03-2010, 09:12 PM n gin nht l dng HPLC anion NO3- v NO2Thn H S Phc 10-03-2010, 09:40 PM Ci khng phi ch no cng c iu kin. V li cc phng php gii thiu trn c th tin hnh hu ht cc phng th nghim v ph hp vi kh nng nghin cu ca sinh vin! Hi nguyencyberchem 10-03-2010, 09:55 PM Ci khng phi ch no cng c iu kin. V li cc phng php gii thiu trn c th tin hnh hu ht cc phng th nghim v ph hp vi kh nng nghin cu ca sinh vin! Hi Th ng ri, ti cu hi chung chung qu, hn na ty iu kin hm lng th no mi chn c pp ph hp, vd nh hm lng nh qu th rt kh chun c nn ci chng ta cn tho lun cht lng hn chnh l nhng cu hi c th hn Thn Gaushinichi 10-05-2010, 06:36 PM Mnh cng mun tm hiu v vn ny!!!!Nu nguyn liu ca mnh l tht th phng php phn tch no l ti u???Mong cc bn gip !!!! diepgl Theo mnh nn dng pp cc ph l tt nht v mu ca bn kh x l X l mu: phng php nghin v phng php ngm chit, hai phng php ny u c nhng hn ch nht nh. phng php nghin lm cho dch nghin c mu nh hng n php xc nh nitrat. Phng php ngm t b nh hng ca mu nhng thi gian ngm chit lu do ko di thi gian phn tch. phng php chit mi l phng php chit dng nng lng vi sng. Khi nim v vi sng: Vi sng theo thut ng ting Anh "microwave" l cc sng cc ngn hay cn gi l sng vi ba c bc sng t 1 mm n 1m. Nng lng ca vi sng l nng lng in t. Tn s ca vi sng thng c s dng trong cng nghip, y t v khoa hc l 915 MHz, 2450 MHz, 5800 MHz, v 22125 MHz. Tn s 2450 MHz (tng

ng bc sng 12,2 cm) c s dng nhiu nht trong cc thit b dn dng v cc thit b chun b mu cho phn tch. Nng lng vi sng c pht ra t mt ngun pht sng in t. Bn cht ca vi sng l sng in t gm hai yu t : yu t t trng B v yu t in trng E. Qu trnh chuyn ho nng lng in t thnh nng lng nhit bao gm hai c ch + C ch chuyn dn ion + C ch quay cc phn t. S t nng bng k thut vi sng da trn s hp th trc tip nng lng vi sng ca mu, do vy cc hin tng nh dn nhit, i lu nhit v bc x nhit ch ng vai tr th yu trong qu trnh cn bng nhit. nh gi kh nng chit ca l vi sng chng ti tin hnh trn mu rau ci c chun b nh sau Chun b mu : Mu rau ci c ra sch sau c thi nh v trn u. Cn mi mu 10 g cho vo cc cc nh mc 250 ml, thm nc ct vo mi cc n khong 200 ml. Chng ti nghin cu kh nng chit ca l vi sng cc mc nng lng khc nhau v thi gian un vi sng cc mc nng lng y, kt qu cho thy: + Mc nng lng un vi sng cng cao th cng chit nhanh nitrat ra khi mu. + Vi mc nng lng cao th ch cn 7 pht chit ht nitrat ra khi mu. So snh vi mt s phng php chit thng thng ( nghin, ngm) chng ti thy phng php chit bng nng lng vi sng c mt s u im sau: + Thi gian chit nhanh + Dch chit khng c mu thun li cho php xc nh nitrat + Hiu sut chit cao hn so vi mt s phng php chit thng thng. + Thit b d s dng, an ton v bo v mi trng. Sau khi chit, xc nh hm lng nitrat trong dch chit th phng php ph bin nht hin nay l phng php trc quang v phng php ny n gin v c nhy kh cao i vi ion nitrat. Tuy nhin xc nh nitrat bng phng php ny trc ht phi tin hnh c cn mu. Phng php c cn thng c s dng trc y l c cn cch thu. Chng ti dng l vi sng (vi mc nng lng trung bnh) c cn dch chit rau v so snh vi c cn cch thu cho kt qu tng ng. Phng php chit nitrat v c cn bng vi sng sau xc nh bng phng

php o quang c so snh vi phng php sc k ion cho kt qu tng i ph hp ajingonsaurus 12-10-2010, 04:06 PM CHo e hi cht. Phn tch NO3 (mang tnh cht theo di vi hm lng NO3 = 0,3 n 1,3mg/L v lng hu c di 1,3 mg/L) bng tia UV 220nm vi my DR5000. Hm lng hu c nh trn c nh hng nhiu n kt qu khng. Nu c, c th cho e hng khc phc c khng. Thank and best regards Tokyo Hi cc bn, Phn tch Nitrite, Nitrate c th dng sc kh ion vi u do UV-Vis, Conductivity,hay MS. Ty nhu cu m mnh chn u d thch hp. Theo mnh bit th v phn tch ion th hng Dionex l mnh nht, v cng ngh thit b ln nghin cu cc ng dng. Cc bn c th vo trang web ca Dionex: www.dionex.com m search, s c rt nhiu application note v nitrate, nitrite v nhiu th khc ah, c th ti v c. chc mi ngi sc khe!