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TRNG I HC Y - DC
BO CO TNG KT TI NCKH CP B
THIT K VECTOR MANG GEN M HO PROTEIN B MT CA VIRUT H5N1 PHC V NGHIN CU CHUYN GEN TO VACCINE THC VT M s:B2010-TN04-01
Ch nhim ti: ThS. Nguyn Thu Hin
* Vaccine nhc c (attenuated vaccine) * Vaccine bt hoat (inactivated vaccine) * Vaccin th h mi: - vaccine tai t hp (recombination vaccine) - vaccine thc vt (plant-base vaccine), ...
2. Ni dung nghin cu
- Thit k gen m ha protein b mt ca virus H5N1 vo vector
2. Phng php nghin cu - K thut PCR,colony PCR - Cc phng php bin nap: sc nhit,xung in
Khng nguyn HA
THIT K VECTOR
June 12
Protein
B epitope
57
G
6
T epitope
60
June 12
Ti liu chiavector tai t hp cha cu trc gen HAop Bc 1:Thit k s ti: wWw.SinhHoc.edu.vn
Tn mi XhoI-HA HindIII-HA Trnh t CCGCTCGAGATCTGCATTGGATACCACGCT CCCAAGCTTGCAGATCCTGCACTGAAGAGAT
1.7kb
Ti liu chia vector tai t hp cha cu trc gen HAop s ti: wWw.SinhHoc.edu.vn Bc 1:Thit k
Ti Bc 1:Thit k vector tai t hp cha cu trc gen HAop liu chia s ti: wWw.SinhHoc.edu.vn
in di kim tra vector tai t hp pPhaso-HAop bng PCR (A)v ct bi enzyme hn ch EcoRI (B). M: Thang chun 1 Kb; 1, 2, 3: mu vector tai t hp tach t cac dong khun lc.
Kim tra dong A. tumefaciens CV58 mang gen bin nap bng PCR
Kim tra gen chuyn HAop trong cy thuc la. M: Thang chun 1 Kb; 1: mu cy thuc la khng chuyn gen; 2, 3, 4, 5, 6 va 7: mu cy thuc la chuyn gen HAop.
Hat u tng
Gy nhim 40
Ra cy trn ct/tru
1
2 3
250
220 180 650
80
70 55 205
30
25 20 65
16
25 14 45
12
10 10 32
Tng
thit k thnh cng vector mang cu trc gen ph hp biu hin thc vt.
2. thu c cy thuc la mang cu truc HAop y la ngun nguyn liu cho phn tch kh nng biu hin gen chuyn va c s cho bin nap vao cac cy trng khac.
nguyn ca virus H5N1 lm vacine n c cho gia cm phng chng bnh H5N1
Vit Nam.