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Lp K14S1_Nhm 2_t 5
MC LC
CHNG I: PROTEIN.......................................................................................................................................2
BI 1: PHN NG BIURE.........................................................................................................................2
BI 2: KT TA THUN NGHCH BNG MUI TRUNG TNH.........................................................4
BI 3: PHNG PHP SORENSEN.........................................................................................................6
(PHNG PHP XC NH M FORMOL )..............................................................................................................6
BI 4: NH LNGPROTEIN BNG PHNG PHP KJELDAHL..................................................7
(GING TRN M HNH).....................................................................................................................................7
CHNG II: ENZYME......................................................................................................................................9
BI 1: NH HNG CA NHIT N HOT TNH CA ENZYME..........................................9
BI 2: NH HNG CA CC CHT HOT HA V KM HM N HOT TNH CA
ENZYME...................................................................................................................................................10
BI 3: XC NH HOT -AMYLASE THEO PHNG PHP WOHLGEMUTH...................11
CHNG III: GLUCIDE.................................................................................................................................13
BI 1: PHN NG VI THUC TH FEHLING..................................................................................13
BI 2: PHN NG SELIWANOFF..........................................................................................................15
BI 3: XC NH NG KH BNG PHNG PHP QUANG PH K....................................17
CHNG IV: VITAMIN..................................................................................................................................19
BI 1: PHNG PHP NH TNH VITAMIN A.................................................................................19
BI 2: PHNG PHP NH TNH VITAMIN C.................................................................................20
BI 3: PHNG PHP NH LNG VITAMIN C.............................................................................21
BI 1: S NH TNG HA LIPIDE...................................................................................................23
BI 2: PHN NG X PHNG HA.....................................................................................................24
BI 3: XC NH CH S IOD CA CHT BO.................................................................................25
BI 4: XC NH CH S ACIDE CA CHT BO............................................................................27
BI 5: XC NH LIPIT TNG S........................................................................................................28
(GING TRN M HNH)...................................................................................................................................28
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Lp K14S1_Nhm 2_t 5
Chng I: PROTEIN
Bi 1: PHN NG BIURE
Nguyn tc:
ng nghim
ng 1 (ng biure)
Dung dch tm
Dung dch mu tm
PTP:
(CO-NH-)+ Cu(OH)2
Ch khng cho qu nhiu CuSO4 v mu xanh ca Cu(OH)2 c to thnh
Gii thch:
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Lp K14S1_Nhm 2_t 5
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Lp K14S1_Nhm 2_t 5
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Lp K14S1_Nhm 2_t 5
Gii thch :
Mi loi protein kt ta cc nng khc nhau .Khi cho NaCl vo ,u tin Globulin
kt ta trc .Sau lc ht kt ta ,cho vo dch lc CH3COOH 1%, pH gim lm xut
hin kt ta Albumin.
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Lp K14S1_Nhm 2_t 5
khi dng).
Ln 1
Ln 2
Ln 3
Trung bnh
Th khng
Th tht
0,4
0,5
0,4
V1= 0,45ml = 0,00045 l
10,6
10,7
10,5
V2= 10,6ml = 0,0106 l
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Lp K14S1_Nhm 2_t 5
V c ha mu :
R-CHNH2 COOH + H2SO4 CO2 + H2O + (NH4)2SO4
Ct m :
(NH4)2SO4 + 2 NaOH Na2SO4 + 2NH3 + 2H2O
H2SO4m c.
Se.
NaOH 40%.
Phenolphtalein 1% trong cn .
HCl long .
(Nn dng ng chun chun b H2SO4 v NaOH 0,1N)
Lp K14S1_Nhm 2_t 5
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Lp K14S1_Nhm 2_t 5
1 pht
2 pht
4 pht
6 pht
8 pht
12 pht
ng 1
ng 2
ng 3
ng 4
Xanh en
Xanh en
Xanh en
Xanh en
Xanh en
Xanh en
Tm en
en nht
Nu en
Nu en
Nu t
Nu en
en
Tm en
Nu nht
Nu nht nht
Nu nht hn
Nu rt nht
en m
Nu t
Nu t nht
Nu t nht
Nu t nht hn
Mu ca lugol
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Lp K14S1_Nhm 2_t 5
hn, enzyme b bin tnh, t khi c kh nng c hi phc li hot . Ngc li, di
00C, hot enzyme tuy b gim nhng li c th tng ln khi a v nhit bnh thng
ng nghim
ng 1
1ml nc ct
Mu vng hi m
ng 2
1ml dd NaCl 1%
Mu vng nht hn
ng 3
1ml dd CuSO4
Mu xanh en
Kt qu:
Ta thy enzyme ha tan tt trong nc, trong dung dch mui long nhng khng tan
trong dung mi khng phn cc
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10
Lp K14S1_Nhm 2_t 5
Cc amylase l cc enzyme thy phn tinh bt, c 2 loi l -amylase v amylase. S tc dng ca hn hp -amylase v -amylase trn tinh bt cho ta hn hp
cng lc cng nhiu glucose, maltose v dextrin n gin
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Lp K14S1_Nhm 2_t 5
Kt qu:
Ta thy 6 ng nghim u thy phn hon ton. ng 7 phn hy c , cn 3 ng cn
li hon ton khng b phn hy
Hot enzyme:
X= 2n *2
(trong n l s th t ng nghim c nng enzyme thp nht v c thy phn
hon ton)
X= 26 *2=128
Gii thch:
Khi chng ta pha long nc bt 10 ln v dch enzyme trong nc bt li c pha long
n 10 ln tip theo (qua 10 ng nghim) th n ng nghim 7, lng tinh bt cn rt t
nn ch thy phn c . n 3 ng nghim cui (ng nghim 8,9,10), lng enzyme
khng cn nn khng th thy phn c tinh bt
Vai tr ca NaCl trong th nghim: Cl- trong NaCl lm cht hot ha
enzyme
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Lp K14S1_Nhm 2_t 5
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Lp K14S1_Nhm 2_t 5
Gii thch:
Thuc th Fehling l hn hp 2dd : dd CuSO4
v dd mui Seignet vi NaOH. Khi trn 2 dd trn
vi nhau th xy ra phn ng :
CuSO4 + 2 NaOH Cu(OH)2 + Na2SO4
Sau dd CuSO4 tc dng vi mui seignet
to mui phc ha tan, dd c mu xanh thm
Mui phc trn l hp cht khng bn
Trong mi trng kim, cc mono v 1 s
dixacarit kh Cu2+ di dng alcolat ng thnh
Cu2+, chc aldehit b oxi ha thnh axit hoc
mui tng ng
Do glucose v maltose c tnh kh nn khi
un vi dd thuc th Fehling th kt ta ca
Cu2O hnh thnh ( do ng kh Cu(OH )2 c
trong Fehling thnh CuO2 )
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Lp K14S1_Nhm 2_t 5
Bi 2: PHN NG SELIWANOFF
Nguyn tc:
Kt qu
ng 1
C mu anh o ti
ng 2
Mu vng nht
ng 3
Gii thch :
Phn ng seliwanoff c trng i vi cc fructose v cc cetohexose nhng khng
nhy i vi cc aldohexoz
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Lp K14S1_Nhm 2_t 5
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Lp K14S1_Nhm 2_t 5
Bi 3: XC NH NG KH BNG PHNG
PHP QUANG PH K
Nguyn tc:
Chun b mu:
1g nguyn liu thm + H2SO4 10% (ngp mu), un cch thy khong 1h,
ly ra cho NaOH vo trung ha n pH=7, lc nu c cn ta c dung dch mu
Chun b mu:
1g nguyn liu thm + H2SO4 10% (ngp mu), un cch thy khong 1h,
ly ra cho NaOH vo trung ha n pH=7, lc nu c cn ta c dung dch mu
1
0
1
2
0
1
3
0,05
0,95
4
0,1
0,9
5
0,2
0,8
6
0,4
0,6
7
0,6
0,4
12
1
17,994
2
20,337
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17
Lp K14S1_Nhm 2_t 5
hp thu
1.2
1
0.8
0.6
0.4
0.2
0
0
10
15
20
25
Nng
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Lp K14S1_Nhm 2_t 5
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Lp K14S1_Nhm 2_t 5
Dd Vitamin C.
Xanh metylen 0,01%,
Dd NaOH 5%.
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Lp K14S1_Nhm 2_t 5
dd HCl 5%.
dd I2 0,01N.
dd tinh bt 1% .
Chanh tri ,bi ,cam.
Trong :
0,5
0,5
0,6
V= 0,533ml
0,533.50.0,00088 .100
= 0,023452 %
20.5
Phn ng oxy ho acide ascorbic :
X(%) =
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21
Lp K14S1_Nhm 2_t 5
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22
Lp K14S1_Nhm 2_t 5
Chng V: LIPIDE
Bi 1: S NH TNG HA LIPIDE
Nguyn tc:
ng 1
ng 2
Kt qu
Gii thch
To thnh 2 lp
,lp du ni ln
trn.(l nhng
ht du khng
nhp vo nhau
ni ln trn).
Chuyn thnh
trng c ,
mt thi gian s
phn lp .
Bn cht l protein
nh tng mnh hn r
ng
- 2010 -
Cho mt r ng vo th
do trong r ng do c
mui mt l cht gy nh
tng nn xy ra hin
tng nh tng nhng
yu hn ng 1
23
Lp K14S1_Nhm 2_t 5
Bi 2: PHN NG X PHNG HA
Nguyn tc:
Gii thch:
Di tc dng ca enzyme, axit hoc kim, un nng, m b thy phn to thnh
glixerin v axit bo hoc mui ca axit bo bc cao gi l x phng
Phn ng nh sau :
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Lp K14S1_Nhm 2_t 5
Bi 3: XC NH CH S IOD CA CHT BO
Nguyn tc:
Du n
Cn 96o
Dd I2 0,1N
Dd Na2S2O3 0,1N dng chun
Dd tinh bt 1%
1ml H2O ct
Bnh
(1)
(2)
2g Du n
(3)
(4)
(5)
(6)
9,7
9,7
9,8
9,7
9,6
9,7
Thm vo mi bnh 1ml dung dch tinh bt 1%, v tip tc chun n khi mt
mu xanh
Na2S2O3 0,1N dng chun
(ml)
0,4
0,3
0,4
0,5
0,3
0,4
Tnh ch s iod:
a: s ml Na2S2O3 0,1N dng chun mu trng
9,7 + 9,7 + 9,8 0,4 + 0,3 + 0,4
+
= 10,1 (ml)
a =
3
3
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Lp K14S1_Nhm 2_t 5
Ch s I2 =
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26
Lp K14S1_Nhm 2_t 5
Bi 4: XC NH CH S ACIDE CA CHT BO
Nguyn tc:
Du n tinh luyn ,
Cn tuyt i 980 .
Dd KOH 0,1N chun .
Ch th phenolphthalein 1%
34,5
35
34
V= 34,5ml
Ch s acid cho bit c cht lng cht bo, ch s acid cng cao chng
t cht bo khng ti, b phn hy v b oxi ha mt phn
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27
Lp K14S1_Nhm 2_t 5
Bi 5: XC NH LIPIT TNG S
(Ging trn m hnh)
Nguyn tc:
C 2 phng php xc nh :
Phng php xc nh trc tip : chit xut lipide ra khi nguyn liu v
cn trc tip
Phng php xc nh gin tip : chit xut lipide ra khi nguyn liu v
cn li nguyn liu
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Lp K14S1_Nhm 2_t 5
Thit b Soxhlet :
Lp tr chit vo bnh cu
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29
Lp K14S1_Nhm 2_t 5
Tnh kt qu
Tnh kt qu
(a b).100
c
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30